CA2180830A1 - Anti-annexin-v monoclonal antibody, process for producing the same, and use thereof - Google Patents

Anti-annexin-v monoclonal antibody, process for producing the same, and use thereof

Info

Publication number
CA2180830A1
CA2180830A1 CA002180830A CA2180830A CA2180830A1 CA 2180830 A1 CA2180830 A1 CA 2180830A1 CA 002180830 A CA002180830 A CA 002180830A CA 2180830 A CA2180830 A CA 2180830A CA 2180830 A1 CA2180830 A1 CA 2180830A1
Authority
CA
Canada
Prior art keywords
annexin
monoclonal antibody
antigen
cell lines
hybridoma cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002180830A
Other languages
French (fr)
Other versions
CA2180830C (en
Inventor
Ryuko Matsuda
Tadahiro Kajita
Yohsuke Ohta
Noboru Kaneko
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority claimed from PCT/JP1995/002305 external-priority patent/WO1996015152A1/en
Publication of CA2180830A1 publication Critical patent/CA2180830A1/en
Application granted granted Critical
Publication of CA2180830C publication Critical patent/CA2180830C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
    • G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
    • G01N2333/4701—Details
    • G01N2333/4718—Lipocortins

Abstract

Using human annexin-V or human annexin-V plus dog annexin-V
as antigen(s), hybridoma cell lines are prepared which are capable of producing anti-annexin-V monoclonal antibodies having a binding specificity to antigenic determinant site on annexin-V
as antigenic protein and belonging to immunoglobulin G class. By the hybridoma cell lines are produced the anti-annexin-V
monoclonal antibodies, with which a diagnostic agent is provided for diagnosis of myocardial infarction and angina pectoris.
There is also provided diagnosis of myocardial infarction and angina pectoris using a first and a second monoclonal antibodies produced by the hybridoma cell lines to quantitate human annexin-V in a sample, in which an antigen-antibody reaction on annexin-V in the sample is caused with the first anti-annexin-V
monoclonal antibody to form an annexin-V antigen/anti-annexin-V
monoclonal antibody complex, the antigenic site of annexin-V of the formed annexin-V antigen/annexin-V monoclonal antibody complex is allowed to be bound with a labeled anti-annexin-V
polyclonal or second monoclonal antibody so as to form a labeled form of said annexin-V antigen/anti-annexin-V monoclonal antibody complex bound with the polyclonal or the second monoclonal antibody, and the labeled form of the complex is quantitatively analyzed.
CA002180830A 1994-11-11 1995-11-10 Anti-annexin-v monoclonal antibody, process for producing the same, and use thereof Expired - Fee Related CA2180830C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP31395894 1994-11-11
JP313958/1994 1994-11-11
PCT/JP1995/002305 WO1996015152A1 (en) 1994-11-11 1995-11-10 Antiannexin-v monoclonal antibody, process for producing the same, and use therof

Publications (2)

Publication Number Publication Date
CA2180830A1 true CA2180830A1 (en) 1996-05-23
CA2180830C CA2180830C (en) 2006-10-31

Family

ID=37395750

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002180830A Expired - Fee Related CA2180830C (en) 1994-11-11 1995-11-10 Anti-annexin-v monoclonal antibody, process for producing the same, and use thereof

Country Status (1)

Country Link
CA (1) CA2180830C (en)

Also Published As

Publication number Publication date
CA2180830C (en) 2006-10-31

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Effective date: 20121113