CA2178361A1 - Ethylamido fluorenes and improved method of making same - Google Patents

Abstract

Novel substituted fluorene compunds of Formula I are active as melatonergic agents:

(I) wherein:
X= H, halogen, OH or OZ;
Z= C1-6 alkyl;-(CH7)m-CF3 (m=0-2); CD3; or (m'=1-3);

n=1 or 2; and R= C1-6 alkyl, C3-6 cycloalkyl, C2-4 alkenyl, halogen substituted C1-6 alkyl, or C1-6 alkoxy substituted C1-6 alkyl.

Description

~17 ~ 3 61 CT-2311A/BMMC7A

This application discloses novel fluorene compounds having amidoethyl substituents at the Cg position. It also concerns the preparation of these compounds, as well as methods and compositions which use them. The compounds have melatonergic properties that are 10 believed to make them useful in treating sleep disorders, e.g., jet-lag and the like.

Melatonin (N-acetyl-5-methoxytryptamine) is a hormone synthesized and secreted primarily by the pineal gland. Melatonin levels 15 show a cyclical, circadian pattern with highest levels occurring during the dark period of a circadian light-dark cycle. Melatonin is involved in the transduction of photoperiodic information and appears to modulate a variety of neural and endocrine functions in vertebrates, including the regulation of reproduction, body weight and metabolism in photoperiodic 20 m~mm~ls, the control of circadian rhythms and the modulation of retinal physiology.

Recent evidence demonstrates that melatonin exerts its biological effects through specific receptors. Use of the biologically active, 25 radiolabelled agonist [l25I]-2-iodomelatonin has led to the identification of high affinity melatonin receptors in the central nervous systems of a variety of species. The sequence of one such high affinity melatonin receptor, cloned from frog dermal melanophores, has been reported (Ebisawa, et al., Proc. Natl. Acad Sci. 91: 6133-6137, 1994). In mammalian 30 brain, autoradiographic studies have localized the distribution of melatonin receptors to a few specific structures. Although there are significant differences in melatonin receptor distribution even between closely related species, in general, the highest binding site density occurs in discrete nuclei of the hypothalamus. In humans, specific [l25I]-2-35 iodomelatonin binding within the hypothalamus is completely localizedto the suprachiasmatic nucleus, strongly suggesting the melatonin receptors are located within the human biological clock.

~ 1 7 8 3 61 CT-2311A/BMMC7A

Exogenous melatonin administration has been found to synchronize circadian rhythms in rats (Cassone, et al., J. Biol. Rhythms, 1:
219-229, 1986). In humans, administration of melatonin has been used to 5 treat jet-lag related sleep disturbances, considered to be caused by desynchronization of circadian rhythms (Arendt, et al., ~r. Med. J. 292:
1170, 1986). Further, the use of a single dose of melatonin to induce sleep in humans has been claimed by Wurtman in International Patent Application WO 94/07487.
Melatonin binding sites have been found in several diverse tissues of the body--i.e., in the retina, superchiasmatic nucleus, spleen, etc. Thus, melatonin exerts multiple physiological effects, is not highly selective, and has a significant potential for producing side effects. Melatonin agonists 15 should be more selective than melatonin and give fewer side effects.

In addition, melatonin's metabolic profile can be problematic in that the compound degrades rapidly in vivo and its oral bioavailability is often low and variable. Suitable melatonin agonists could overcome 20 these drawbacks, resulting in products having more predictable activity.

Thus, melatonin agonists should be particularly useful for the treatment of sleep disorders and other chronobiological disorders.
Melatonin agonists would also be useful for the further study of 25 melatonin receptor interactions as well as in the treatment of conditions affected by melatonin activity, such as depression, jet-lag, work-shift syndrome, sleep disorders, glaucoma, reproduction, cancer, immune disorders, and neuroendocrine disorders.

30U.S. Patent 5,206,377 to McAfee discloses compounds having melatonin antagonist activity which conform to formula 1:

R ~ R,.

~ 1 7 8 ~ 6 ~ CT-2311A/BMMC7A

wherein R1 is C1~ alkanoyl; R1 is hydrogen, C1 6 alkyl or optimally substituted phenyl; R2 is hydrogen or phenyl substituted C1~ alkylene;
and R3, R4, Rs and R6 are Cl 6 alkyl, Cl~ alkoxy or optionally substituted phenoxy. The McAfee compounds do not contain N-amidoethyl 5 substituents.

Stamm, et al., at Chem. Ber. 111: pp. 2665-6 (1978), show the amidoethylation of fluorene with N-acylaziridines to yield compounds of formula 2:
~3 HNC(O)Z

wherein Z is ethoxy, diphenylamine, diethylamine or phenyl.

Assithianakis et al., disclose compounds of formula _ in Arch.
Pharm. Vol. 320, (1987), pp. 604-8:
,~
H3C>~ z ~ NHCO~R

Z=HorOH;andR=HorBr.

Hansen et al. disclose, in EPO Patent publication 0215297A2, compounds of formula 4:
Ar I 1 11 1 /\ I
R4 R5 O R6 R7 Rs ~alkylene)-9H-fluoren-9-yl wherein Ar is an optionally substituted phenyl group, and R4 through R9 are H or lower alkyl. These dipeptides are used as analgesics.

~ 17 8 3 61 CT-2311A/BMMC7A

Severin et al., show in Chem. Ber. Vol. 110, (1977), p. 491-8, the preparation of the fluorenyl ethyl amine of formula 5:

None of these publications discloses the compounds of this inventlon.

The invention is concerned with substituted fluorenyl compounds of formula I and compositions and methods which employ them.
lS
Formula I is:

(¦~ H
zo ~ x (I) wherein:
X= H, halogen, OH or OZ;
Z= Cl~ alkyl; -(CH2)m-CF3 (m=0-2); CD3; or O-alkyl --(CH2)m ~ (m'=1-3);
n=1 or 2; and R= C1 6 alkyl, C3~ cycloalkyl, C2~ alkenyl, halogen substituted C1 6 alkyl, or C1 6 alkoxy substituted Cl 6 alkyl.
The melatonergic agents of the invention have several advantages over similar agents. They perform well in tests which demonstrate affinity for the melatonin binding site found in human suprachiasmatic ~2178361 nucleus (SCN). Many of the compounds have ICs~ values for melatonin binding of 250 nM or less.

The instant compounds are agonists as determined by their 5 melatonin-like ability to block the forskolin-stimulated accumulation of cyclic AMP in certain cells. Also, many of these compounds are able to affect activity rhythms in rodents, indicating the ability to moderate circadian rhythms in mammals.

These ànd other advantages will become more apparent after consideration of the specification and claims.

The new melatonergic agents described herein conform to formula I:

( ~ N R
zo ~ x (I) wherein:
20 X= H, halogen, OH or OZ;
Z= C1~ alkyl; -(CH2)m-CF3 (m=0-2); CD3; or ~ O-alkyl ~ (CH2)m ~ (m'=1-3);
n=lor2;and R= C1 6 alkyl, C3-6 cycloalkyl, C2 4 alkenyl, halogen substituted C1 6 alkyl, 2~ or Cl 6 alkoxy substituted C1 6 alkyl.

By "alkoxy", applicants mean alkoxy groups, -O-alkyl, having branched or stralght chains. Methoxy groups are among the preferred alkoxy groups.
By "alkyl" is meant branched or straight chain Cn H(2n +l) group moieties and cyclic Cn H(2n~-l) moieties. n' is the number of C atoms in ~1 7 ~ 3 6 ~ CT-2311A/BMMC7A
these moieties. All alkyl groups in R or Z, except for cyclic ones, contain from 1 to 6 carbon atoms. Preferred alkyl groups include methyl, ethyl, isopropyl, n-propyl, cyclopropyl, and cyclobutyl.

"Alkenyl" denotes monovalent straight or branched chain moieties containing one site of unsaturation and at least 2 carbon atoms.
These moieties conform to the formula Cn H(2n~-1), with n" being the number of carbon atoms present. Preferred alkenyl moieties include vlnyl.
Alkoxy substituted phenyl groups in compounds of the invention will contain from 7 to 10 carbon atoms. They may be linked to a ring of the fluorene moiety via 1 to 3 methylene (-CH2-) groups. (Methoxy phenyl)propyl substituents are preferred.
Alkoxy substituted alkyl groups found in compounds of the invention contain a total of 2 to 8 carbon atoms. Any of the alkyl groups may be straight, branched or cyclic, as set out above. Preferred groups of this type include methoxymethyl.
The term "halogen" refers to Cl, Br, F or I atoms. Generally, there will be from 1 to 3 halogen substituents present in each halogen substituted alkyl moiety. Preferred halogen substituents include Cl and F.
Halomethyl groups, e.g., chloromethyl and trifluoromethyl groups are preferred.

"D" refers to deuterium m can be 0, 1 or 2 and designates the number of CH2 groups. m is preferably 1.

m' refers to 1, 2 or 3 and is preferably 3.

n is 1 or 2, preferably 1.
One preferred group of formula I compounds includes those wherein Z is a methyl group and X is hydrogen or a methoxy group.

~ :17 8 3 ~ ~ CT-2311A/BMMC7A

Among these, those in which R is methyl, n-propyl, methoxymethyl, i-propyl and vinyl are preferred. Some compounds in this group are:
N-[2-(2-methoxyfluoren-9-yl)ethyl]butanamide;
N-[2-(2-methoxyfluoren-9-yl~ethyl] acetamide;
N-[2-(2-methoxyfluoren-9-yl)ethyl]-2-propenamide;
N-[2-(2,7-dimethoxyfluoren-9-yl)ethyl]methoxyacetamide; and N-[2-(2,7-dimethoxyfluoren-9-yl)ethyl]-2-methyl propanamide.

A second preferred group of compounds of formula I are compounds in which Z is a methyl group and X is a methoxy group.
Included in this group are:
N-[3-(2,7-dimethoxyfluoren-9-yl)-prop-1-yl]propanamide;
N-[3-(2,7-dimethoxyfluoren-9-yl)-prop-1-yl]butanamide;
N-[3-(2,7-dimethoxyfluoren-9-yl)-prop-1-yl]acetamide;
N-[3-(2,7-dimethoxyfluoren-9-yl)-prop-1-yl]cyclobutane carboxamide;
N-[3-(2,7-dimethoxyfluoren-9-yl)-prop-1-yl]cyclopropane carboxamide;
N-[2-(2,7-dimethoxyfluoren-9-yl)-ethyl]but-2-enamide; and N-[2-(2,7-dimethoxyfluoren-9-yl)-ethyl]cyclopentane carboxamide.

Among the compounds in the second group, the following are more preferred:
N-[2-(2,7-dimethoxyfluoren-9-yl)ethyl]butanamide;
N-[2-(2,7-dimethoxyfluoren-9-yl)ethyl]propanamide;
N-[2-(2,7-dimethoxyfluoren-9-yl)ethyl]cyclopropane carboxamide;
N-~2-(2,7-dimethoxyfluoren-9-yl)ethyl]acetamide;
N-[2-(2,7-dimethoxyfluoren-9-yl)ethyl]cyclobutane carboxamide; and N-[2-(2,7-dimethoxyfluoren-9-yl)ethyl]choroacetamide.

A third preferred group are compounds wherein Z is a methyl group, X is alkoxy or alkoxyphenylalkyl and R is alkyl.

Yet another preferred group are compounds wherein X=OZ=OCH2CF3.

Compounds of formula I also encompass all solvates, particularly hydrates, thereof.

217836~

The invention also encompasses geometric and optical isomers which arise as a consequence of structural asymmetry. Separation of individual isomers is accomplished by the application of various methods known to practitioners in the art.

The compounds of the invention are made using the following general scheme:
General Synthetic Scheme CN
zo~, (EtO)20PCH2CN _~,X

NaH, THF

PtO2 / ¦ NaH, DMSO
/ CHCI3 Me3S(O)I
--NH2~HCI EtOH CN
zo~,X zo~,X

Et3N
CH3CN 1) Raney-Ni. H2 , RCOCI EtOH, EtOAc, NH40H
O 2)HCI
~ N~ Et3N 1~ NH2-HCI
ZO~3~3,X RCOCI Z~3, .
n = 1 or 2 X = H, halogen or alkoxy Z= C1~ aL~cyl; -(CH2)mCF3; CD3; or--(CH2)m' ~ O-alkyl 15 THP = tetrahydrofuran The appropriate fluorenone is converted to the cc"~-unsaturated nitrile which is then catalytically reduced to give the ethylamine hydrochloride. Alternatively, the a, ~-unsaturated nitrile can be - ~1 7 8 3 6~ i CT-2311A/BMMC7A

cyclopropanated and then reduced to provide the propylamine hydrochloride. Either type of amine is then converted to the desired amide using any one of a variety of acylating conditions. The use of this scheme is described in greater detail below.

The fluorenones are puchased commercially or prepared by the following general scheme:
General Synthesis of Fluorenones Pd(dba)3 YO~ CO2Me ~¢~ ~ yo~ C2Me Br (HO)2B Na2CO

Y=HorMe 1) when Y= H, then alk~late with R'X, base, 2)1N NaOH, EtOH CO2H SOCI2 ll R O~_~ 1) SOCI2 ~X

2) AICI3 R'= alkyl or alkylaryl An appropriately substituted o-bromoester and phenylboronic acid are coupled using palladium catalysis to provide a biphenyl intermediate 15 which can be alkylated on oxygen in the cases where a free phenol exists on one of the aromatic rings. The ester is then hydrolyzed to the acid which is cyclized to the desired fluorenone. The use of this scheme is described in greater detail below.

The compounds of the invention may be administered to patients in need of melatonergic treatment i.e., patients suffering from sleep disorders and the like, in a variety of ways. Thus, oral, transdermal, 25 subcutaneous, intravenous, intramuscular, rectal, buccal, intranasal, and ocular routes can be used.

~1 7 8 3 ~i I CT-231 1A/ BMMC7A

One or more of the compounds of the invention is mixed with pharmaceutically acceptable amounts of one or more conventional pharmaceutical excipients to produce a formulation to be administered by the desired route. Generally, such formulations will contain one or 5 several carriers or diluents. Useful carriers include solids, semi-solids and liquids which have miscibility, or other compatibility, with the active agent(s) so that they can deliver same to a patient or host.

Suitable carriers include lactose, dextrose, sucrose, sorbitol, 10 mannitol, starches, gum acacia, calcium phosphate, alginates, tragacanth, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, methyl cellulose, methyl- and propyl-hydroxybenzoates, talc, magnesium stearate, mineral oil and the like.
Mixtures are operable.
Other useful excipients include lubricants, wetting agents, gellants, emulsifiers, preservatives, colorants, perfumes, flavor enhancers, drying agents and the like. Mixtures can be employed.

Generally, compositions which include the compounds of the invention will contain from about 0.10 to about 10% of active compound(s) and 99.9 to 90%, or other suitable amounts, of excipient(s).

Dosage levels will be dictated by the patient's needs and by the medical judgment of the treating physician. Generally, however, dosages of about 0.1 mg to about 100 mg per day are useful to treat sleep or circadian rhythm disorders.

While human patients are most preferred, the compounds of the invention may be used to treat other subjects, i.e., animals preferably mammals.

~17 ~ 3 ~1 CT-2311A/BMMC7A

SPECIFIC EMBODIMENTS

The compounds which constitute this invention, their methods of preparation and their biologic actions will appear more fully from 5 consideration of the following examples, which are given for the purpose of illustration only and are not to be construed as limiting the invention in sphere or scope. In the following examples, used to illustrate the foregoing synthetic processes, temperatures are expressed in degrees Celsius and melting points are uncorrected. The nuclear magnetic 10 resonances (NMR) are spectral characteristics refer to Chemical shifts (~) expressed as parts per million (ppm) versus tetramethylsilane (TMS) as rererence standard. The relative area reported for the various shifts in the lH NMR spectral data corresponds to the number of hydrogen atoms of a particular functional type in the molecule. The nature of the shifts as to 15 multiplicity is reported as a broad singlet (bs), singlet (s), multiplet (m), doublet (d), or triplet (t). Abbreviations employed are DMSO-d6 (deuterodimethylsulfoxide), CDCl3 (deuterochloroform) and are otherwise conventional. The infrared (IR) spectral descriptions include only absorption wave numbers (cm~l) having functional group 20 identification value. The IR determinations were employed using the compound neat as a film or by employing potassium bromide (KBr) as diluent. The elemental analyses are reported as percent by weight.

Unless otherwise noted, all percentages recited herein are weight 25 percents, based on total composition weight.

The following examples describe in detail the preparation of compounds of Formula I. It will be apparent to those skilled in the art that modifications, both of materials and methods, will allow preparation 30 of other compounds disclosed herein. From the foregoing description and the following examples it is believed that one skilled in the art is able to use the invention to the fullest extent.

The appropriate starting materials such as 2-hydroxy-9-fluorenone 35 were purchased from commercial sources; 2,7-dihyroxy-9-fluorenone was prepared according to the methods described by Andrews et al (Journal of h) 17 8 3 g ~ CT-2311A/BMMC7A

Medicinal Chemistry, 1974, 17, 882) and Agarwal (Journal of MedicinaZ
Chemistry, 1967, 10, 99) or purchased from commercial sources.

These examples discuss-methods of making various compounds of formula I and their biological activity.

10 Example 1: 2-[9-(2-Methoxyfluorenyl)]ethylamine hydrochloride: A
solution of 2-hydroxy-9-fluorenone (0.042 mol), potassium carbonate (0.20 mol), and methyl iodide (0.042 mol) in acetonitrile was heated to reflux overnight. The reaction mixture was cooled and the solvent removed in vacuo to yield a solid. This was dissolved in methylene chloride, washed 15 with saturated sodium carbonate solution, dried over MgSO4 and the solvent removed to yield a solid which was identified as 2-methoxy-9-fluorenone. To a suspension of NaH (1.61 g, 0.067 mol) in THF (200 mL) at RT was added via syringe, diethyl cyanomethylphosphonate (7.43 g, 0.042 mol); the reaction was allowed to stir for 15 min after which a pale 20 yellow solution was observed. To this was added dropwise a solution of 2-methoxy-9-fluorenone (8.89 g, 0.042 mol) in THF (100 mL). The reaction was allowed to stir overnight at RT. The solvent was removed in vacuo and the residue was dissolved in methylene chloride, washed with water, dried over MgSO4, and concentrated to yield an orange solid. This was 25 dissolved in acetonitrile and washed with hexane; the acetonitrile solution was concentrated to yield an orange solid shown to be the a,~-unsaturated nitrile by 1H NMR. (79%) A solution/suspension of the a,~-unsaturated cyano compound (7.72 g, 0.033 mol), PtO2 (0.77g), CHCl3 (23 mL), in EtOH (150 mL) was charged with H2 (50 psi) and allowed to 30 shake on a Parr Hydrogenation Apparatus for 18 h. The reaction mixture was then filtered and concentrated in vacuo to yield a white solid. This was washed with Et2O and dried in vacuo to obtain a white solid (69%).

;~'1 7 g 3 ~ ~ CT-2311A/BMMC7A

Example 2: 2-[9-(2,7-Dimethoxyfluorenyl)]ethylamine hydrochloride:
Prepared analogously to 2-[9-(2-Methoxyfluorenyl)]ethylamine hydrochloride in Example 1 beginning with 2,7-dihydroxy-9-fluorenone.
lH NMR (300 MHz, CDC13) ~ 7.60 (d, J=8.0 Hz, 2H), 7.00 (d, J=2.0 Hz, 2H), 5 7.85 (dd, J=8.0, 2.0 Hz, 2H), 3.90-4.10 (m, lH), 3.85 (s, 6H), 2.50-2.60 (m, 2H), 2.10-2.30 (m, 2H). Alternatively, the necessary intermediate 2,7-dimethoxyfluorenone was prepared as follows: Methyl 2-bromo-5-methoxybenzoate (1.60 g, 6.53 mmol), 4-methoxyphenylboronic acid (1.30 g, 8.55 mmol), and tris(dibenzylideneacetone) dipalladium (0) (0.20 g, 0.22 mmol) were added to dimethoxyethane t25 mL) and 2 M sodium carbonate (25 mL). The reaction was stirred at reflux for 16 h then decanted and the residue extracted with ethyl acetate. The combined organic layers were dried (MgSO4) and the solvent removed by rotary evaporation to afford 1.50 g of the coupled product (5.51mmol, 84% yield).
The ester (10.80 g, 39.70 mmol) was hydrolyzed with 1 N sodium hydroxide (80 mL) in refluxing ethanol (500 mL). The cooled reaction mixture was extracted with methylene chloride and then acidified with 1 N hydrochloric acid. The acidic solution was then extracted with methylene chloride. The organic layers were combined and the solvent was dried and concentrated by rotary evaporation to give 9.00 g (34.88 mmol, 88% yield) of the acid. 9.00 g (34.88 mmol) of the acid was dissolved in thionyl chloride (250 mL) and stirred at reflux for 6 h. The reaction was cooled to ambient temperature and the solvent was removed by rotary evaporation to give 8.20 g of 2,7-dimethoxyfluorenone (34.17 mmol, 98% yield) as a red solid.

Example 3: 2-methoxy-7-pentoxyfluorenone. Methyl 2-bromo-5-hydroxybenzoate (9.30 g, 40.26 mmol), 4-methoxyphenylboronic acid (6.54 g, 43.00 mmol), and tris(dibenzylideneacetone) dipalladium (0) (0.30 g, 0.33 mmol) were added to dimethoxyethane (75 mL) and 2 M
sodium carbonate (75 mL). The reaction mixture was stirred at reflux for 16 h then cooled and decanted and the residue washed with ethyl acetate.
The combined organic layers were dried (MgSO4) and the solvent removed by rotary evaporation to afford 9.45 g of the coupled product (36.64 mmol, 91% yield).

~17 8 3 61 CT-2311A/BMMC7A

The coupled product (1.06 g, 4.10 mmol) was alkylated by treating it with pentyl iodide (1.16 g, 5.85 mmol) and potassium carbonate (1.38 g, 10.00 mmol) in DMF at 75 C for 16 h. The cooled reaction mixture was partitioned between ethyl acetate and water. The solvent was removed 5 from the combined organic layers by rotary evaporation to give the alkylated product. The alkylated product was hydrolyzed with 1 N sodium hydroxide (10 mL) in refluxing ethanol (50 mL) until the TLC indicated that the saponification was complete. The cooled reaction mixture was acidified with 1 N hydrochloric acid and extracted with methylene 10 chloride. The organic layers were dried (MgSO4) and concentrated by rotary evaporation to give the acid. The acid was dissolved and warmed in thionyl chloride (50 mL) at 65 C for 30 min. The solution was cooled to ambient temperature and the thionyl chloride was removed by rotary evaporation. The residue was dissolved in methylene chloride (50 mL) and aluminum chloride (0.67 g, 5.00 mmol) added. The reaction was stirred at ambient temperature for 2 h then quenched by adding the reaction mixture to a beaker containing 100 mL lN hydrochloric acid and ice. The methylene chloride layer was separated and the acidic layer was washed with methylene chloride. The combined organic layers were dried 20 (MgSO4) and the solvent removed by rotary evaporation to give 1.05 g of 2-methoxy-7-pentoxyfluorenone (3.55 mmol, 87% yield) as a red solid.
IH NMR (300 Mhz, CDCl3) ~ 7.28 (d, J = 8.3 Hz, 2H), 7.14 (s, 2H), 6.93 (d, J =
8.3 Hz, 2H), 3.96 (q, J = 7.5 Hz, 2H), 3.82 (s, 3H), 1.80 (p, J = 7.6 Hz, 2H), 1.42 (m, 4H), 0.93 (t, J = 7.5 Hz, 3H).

Example 4. 3-[9-(2,7-Dimethoxyfluorenyl)]propylamine hydrochloride.
Sodium hydride (0.6 g, 22 mmol) was washed with hexane and suspended in 20 mL anhydrous THF under a nitrogen atmosphere. Diethyl 30 cyanomethylphosphonate (2.8 g, 16 mmol) was added slowly in portions over 15 min. and the heterogeneous reaction mixture became a clear solution after the addition was complete. The reaction stirred for 30 min at room temperature. The ketone (4.2 g, 16 mmol) dissolved in 30 mL
THF was added in portions and the reaction was refluxed overnight. The 35 crude reaction was cooled to room temperature and then poured into 150 mL water and extracted with several portions of 40 mL CH2Cl2. The combined organic layers were dried over MgSO4 and concentrated in ~t783~i vacuo to give a wine red solid. This a,~-unsaturated nitrile (3.9 g) was used in the subsequent reaction.
Sodium hydride (1.1 g, 44 mmol) was washed with hexane and suspended in 100 mL anhydrous DMSO under a nitrogen atmosphere.
The heterogeneous reaction mixture was stirred well while portions of trimethylsulfoxonium iodide (9.9 g, 45 mmol) were added. The solution was stirred until the foaming subsided. The reaction was cooled to -60C
and a solution of the c~ ~-unsaturated nitrile in 40 mL anhydrous DMSO
was added in portions over 20 min. The reaction was warmed to room temperature and stirred overnight. The crude reaction was slowly poured into saturated NH4Cl followed by the addition of EtOAc. The aqueous layer was extracted several times with EtOAc and the combined organic fractions were dried over MgSO4 and concentrated in vacuo. The resulting light brown oil was purified by flash chromatography (EtOAc/
Hexane gradient) to give the desired nitrile in 43% after 2 steps (2.1 g;
mp= 131-133C; light yellow solid): lH-NMR (300 MHz, CDCl3) ~ 7.58 (m, 2H), 6.91 (m, 3H), 6.42 (m, lH), 3.85 (s, 3H), 3.80 (s, 3H), 2.33 (m, lH), 2.11 (m, 2H); 13C-NMR (75 MHz, CDCl3) ~ lS8.8, 158.7, 144.9, 142.2, 120.2, 117.8, 114.1, 112.9, 106.8, 104.9, 55.5, 34.8, 21.7, 14.8; ~-llR (KBr) 2833, 2237, 1623, 1582, 1470 cm-l; Anal. Calc. for Cl8H15NO2: C, 77.96; H, 5.45; N, 5.05.
Found: C, 77.80; H, 5.49; N, 5.00.
The nitrile (4.3 g) was dissolved in 200 mL of a 1:1 mixture of EtOH/ EtOAc and 20 mL of NH40H. The mixture was hydrogenated in the presence of Raney-Ni for 4hrs (TLC 1:1 EtOAc/ Hex). The crude mixture was filtered, concentrated in vacuo, redissolved in CH2Cl2 and acetonitrile, dried over MgSO4, filtered, and concentrated to dryness in vacuo. The resulting solid was dissolved in MeOH and acetonitrile and concentrated HCl was added (0.52 mL, 17 mmol). After concentrating to dryness, the white solid was triturated with hexane, filtered, and dried.
The product was obtained in 46/O (2.3 g; mp= 218-221C): lH-NMR
(300 MHz, CDCl3) ~ 7.64 (d, 2H, J= 8.3 Hz), 7.14 (d, 2H, J= 2Hz), 6.90 (dd, 2H, J= 8.3, 2.0 Hz), 3.95 (t, lH, J= 5.2Hz), 3.81 (s, 6H), 2.66 (t, 2H, J= 8.8Hz), 2.12-2.05 (9m, 2H), 1.30-1.20 (m, 2H); 13C-NMR (75 MHz, CDCl3) ~ 158.3, 147.9, 133.5, 119.8, 112.8, 110.3, 55.3, 46.3, 28.9, 22.9; FTIR (KBr) 3425, 2946, 1243 cm-l; Anal. Calc. for Cl8H2lNO2-HCl-0.15 H2O: C, 67.03; H, 6.97; N, 4.34;
Found: C, 66.85; H, 7.03; N, 4.21.

~ 1 7 3 3 ~ ~ CT-2311A/BMMC7A

Example 5: General Procedure, N-[2-(2,7-dimethoxyfluoren-9-yl)ethyl]
propanamide. 2-[9-(2,7-Dimethoxyfluorenyl)]ethylamine hydrochloride (0.0083 mol) was suspended in CH3CN (150 ml) and to this was added excess Et3N (0.024 mol). This mixture was allowed to stir until starting 5 material was completely dissolved. Propionyl chloride (0.0083 mol) was then added and the reaction mixture was allowed to stir overnight. The acetonitrile was removed in vacuo. The residue was then washed with excess water and extracted into CH2Cl2. The organic layer was separated, dried (MgSO4), filtered and concentrated in vacuo to obtain a solid which was purified by recrystallization from EtOAc/hexane. m.p. 139-140 C;
Related compounds were purified by silica gel column chromatography or reverse phase HPLC. lH NMR (300 MHz, CDCl3) ~ 7.52 (d, J=8.3 Hz, 2H), 7.06 (d, J=2.4 Hz, 2H), 6.87 (dd, J=8.3, 2.3 Hz, 2H), 4.87 (bs, lH), 3.98 (t, J=5.1 Hz, lH), 3.83 (s, 6H), 3.04 (m, 2H), 2.29 (q, J=6.6 Hz, 2H), 1.96-1.82 (m,2H), 0.92 (t, J=7.6 Hz, 3H); 13C NMR (75 MHz, CDCl3) ~ 173.4, 158.8, 147.7, 133.9, 119.9, 113.0, 110.1, 55.6, 45.7, 36.1, 31.9, 29.5, 9.49; IR (KBr) 3260, 1640, 1240 cm~l; MS (DCI) m/e MH+= 326; Analysis calc'd for C2oH23NO3: C, 73.82; H, 7.12; N, 4.30; found: C, 73.63; H, 7.17; N, 4.22.

~ 17 8 3 ~1 CT-2311A/BMMC7A

Examples 6-31 The following compounds were also prepared by the general procedure outlined in Example 5 using the appropriate amine 5 hydrochloride and acid chloride.

Example Compound MeltingPoint (C) 6 N-[2-(2-methoxyfluoren-9-yl)ethyl] 97-99 butanamide 7 N-[2-(2,7-dimethoxyfluoren-9-yl)ethyl] 152-153 cyclopropane carboxamide 8 N-[2-(2,7-dimethoxyfluoren-9-yl)ethyl] 147 acetamide 9 N-[2-(2,7-dimethoxyfluoren-9-yl)ethyl] 94-95 methoxyacetamide N-~2-(2,7-dimethoxyfluoren-9-yl)ethyl] 126 butanamide 11 N-~2-(2-methoxyfluoren-9-yl)ethyl]-2- 143-145 propenamide 12 N-[2-(2-methoxyfluoren-2-yl)ethyl] 133-135 acetamide 13 N-[2-(2,7-dimethoxyfluoren-9-yl)ethyl] 145-146 cyclobutane carboxamide 14 N-[2-(2,7-dimethoxyfluoren-9-yl)ethyl]- 88-89 2,2-dimethyl propanamide N-[2-(2,7-dimethoxyfluoren-9-yl)ethyl]-2- 145-146 methyl propanamide 16 N-[2-(2,7-dimethoxyfluoren-9-yl)ethyl] 122-123 choroacetamide 17 N-[2-(2,7-dimethoxyfluoren-9- 155-156 yl)ethyl]cyclopentane carboxamide 18 N-[2-(2,7-dimethoxyfluoren-9- 130-132 yl)ethyl]but-2-enamide 19 N-[3-(2,7-dimethoxyfluoren-9-yl)prop-1- 110-113 yl]cyclobutane carboxamide N-[3-(2,7-dimethoxyfluoren-9-yl)prop-1- 139-141 yl]acetamide ~17 ~ i CT-2311A/BMMC7A

Example Compound Melting Point (C) 21 N-[3-(2,7-dimethoxyfluoren-9-yl)prop-1- 88-90 yl]butanamide 22 N-[3-(2,7-dimethoxyfluoren-9-yl)prop-1- 120-122 yl] propanamide 23 N [3-(2,7-dimethoxyfluoren-9-yl)prop-1- 141-142 yl]cyclopropane carboxamide 24 N-[2-(2-fluoro-7-methoxyfluoren-9-yl)- 72-73 ethyl]propanamide N-[2-(2,7-di(methoxy-d3)f~uoren-9-yl)- 141-143 ethyl] acetamide 26 N-[2-(2,7-di(methoxy-d3)fluoren-9-yl)- 138-140 ethyl]propanamide 27 N-[2-(2,7-di(methoxy-d3)fluoren-9-yl)- 133-136 ethyl]cyclopropane carboxamide 28 N-[2-(2,7-di(methoxy-d3)fluoren-9-yl)- 117-120 ethyl]butanamide 29 N-[2-(2-ethoxy-7-methoxyfluoren-9-yl)- 95-98 ethyl]propanamide N-[2-(2-hydroxy-7-methoxyfluoren-9-yl)- 50-60 ethyl] propanamide 31 N-[2-(2,7-diethoxyfluoren-9-yl)- 225-228 ethyl]propanamide 217 ~ 3 6 ~ CT-2311A/BMMC7A

Example 32: N-2-(2-methoxy-7-(1-pentoxy))fluorene-9-yl)ethyl propanamide. Prepared by the general procedure outlined in example 5 using propionyl chloride. lH NMR (300 MHz, CDCl3~ ~ 7.53 (d, J = 8.3 Hz, 2H), 7.02 (s, 2H), 6.88 (d, J = 8.3 Hz, 2H), 4.90 (bs, lH), 3.95 (m, 3H), 3.84 (s, 3H), 3.04 (m, 2H), 2.30 (q, J = 5.3 Hz, 2H), 1.87 (q, J = 7.6 Hz, 2H), 1.77 (m, 2H) 1.43 (m, 4H), 0.93 (t, J = 7.5 Hz, 6H); 13C NMR (75 MHz, CDCl3) ~ 173.8, 158.6, 158.2, 147.6, 134.1, 133.8, 119.8, 113.5, 113.0, 110.7, 110.1, 68.2, 55.5j 45.6, 36.0, 31.7, 29.3, 29.0, 28.1, 22.4, 13.9, 9.4. Anal. Calcd for C24H31NO3-0.25 H2O: C, 74.68; H, 8.23; N, 3.63. Found: C, 74.57; H, 8.20, N, 3.68.

Example 33: N-2-(2-methoxy-7-(3-(3-methoxyphenyl)propox-1-y))fluorene-9-yl)ethyl propanamide. Prepared by the general procedure outlined in example 5 using propionyl chloride. lH NMR (300 MHz, CDCl3) ~ 7.54 (d, J = 8.3 Hz, 2H), 7.21 (t, J = 7.8 Hz, 2H), 7.03 (s, 2H), 6.91-6.73 (m, 4H), 4.92 (bs, lH), 4.01 (m, 3H), 3.90 (s, 3H), 3.85 (s, 3H), 3.06 (q, J = 6.2 Hz,2H),2.82(t,J=7.9Hz,2H),2.28(q,J=6.6Hz,2H),2.12(p,J=7.8Hz,2H) 1.89 (q, J = 7.6 Hz, 2H), 0.95 (t, J = 7.6 Hz, 3H); 13C NMR (75 MHz, CDCl3) 173.4, 158.8, 158.1, 147.7, 143.2, 133.9, 130.4, 128.4, 120.9, 118.8, 114.7, 114.2, 112.5, 112.0, 111.2, 110.2, 109.9, 109.2, 67.3, 56.5, 54.2, 46.5, 36.1, 32.2, 31.9, 30.8, 29.4, 10.4, 8.7. Anal. Calcd for C29H33NO4-0.67 H2Q: C, 73.85; H, 7.34;
N,2.97.Found:C,73.81;H,7.02,N,2.84.

Example 34: Measurement of Melatonergic Binding 1. Reagents (a) 50 mM Tris buffer containing 12.5mM MgCl2 and 2mM E~TA (pH
7.4 at 37C).
(b) Wash buffer: 20mM Tris base containing 2mM MgCl2 (pH 7.4 at room temperature).
(c) Melatonin (10-5 M final concn.).
(d) 2-[l25I]-iodomelatonin (100 pM final concn.).
Source: NEN

~ 1 7 ~ 3 ~1 CT-2311A/BMMC7A

2. Membrane preparation. The receptor cDNA (human ML1A) was subdoned into pcDNA3 and introduced into NIH 3T3 cells using Lipofectamine. Transformed NIH 3T3 cells resistant to geneticin were isolated and single colonies expressing high levels of 2-[125I]-5 iodomelatonin binding were isolated and characterized. Cell pellets werefrozen at -80C for further use. For preparing membrane homogenates, pellets are thawed on ice, and resuspended in TME buffer, Tris base, MgCl2, EDTA (pH 7.4 at 37C), supplemented with aprotinin, leupeptin, and phenylmethlysulfonylfluoride. The cells were then homogenized 10 using a dounce homogenizer, and centrifuged. The resulting pellet was resuspended with a dounce homogenizer in TME and frozen. On the day of assay, the small aliquot was thawed on ice and resuspended in TME
buffer.

15 3. Incubation: 37C for 1 hour. Reaction is terminated by filtration.

The procedure was based on that disclosed in: Reppert, S. M., Weaver, D. R., and Ebisawa, R. (1994), Neuron ~,1177-1185 (1994).

~17 ,~ ~ 6 1 CT-2311A/BMMC7A

The following table sets forth selected Formula I compounds and binding data which demonstrates their usefulness.

Binding Data of Selected Compounds of Formula I

Compound ExampleBinding Affinity4 N-[2-(2,7-dimethoxyfluoren-9-yl)ethyl] 5 +++
propanamide N-[2-(2-methoxyfluoren-9-yl)ethyl] 6 + +
butanamide N-[2-(2,7-dimethoxyfluoren-9-yl)ethyll , 9 + +
methoxyacetamide N-[2-(2-methoxyfluoren-9-yl)ethyl]-2- 11 + +
propenamide N-[2-(2,7-dimethoxyfluoren-9-yl)ethyl] 13 +++
cyclobutane carboxamide N-[2-(2,7-dimethoxyfluoren-9-yl)ethyl]- 14 +
2,2-dimethyl propanamide N-[3-(2,7-dimethoxyfluoren-9-yl)prop-1- 20 + +
yl]acetamide N-[2-(2-ethoxy-7-methoxyfluoren-9-yl)- 29 + +
ethyl]propanamide * +++ = ICso < 10 nM; ++ = ICso < 100 nM; + = ICso < 500 nM

~1783~1 CT-231 lA/ BMMC7A

Example 35: Measurement of Functional Activity Cyclic AMP Accumulation in Intact Cells: Melatonin CELLS:
The media was removed from cell flask and washed with Hank's salt solution or PBS, as appropriate. The cells were detached from flask.
Enough media was added so that the concentration of cells is 4 x 105/ml when counted with a hemocytometer. Dialyzed or heat inactivated fetal bovine serum (FBS) was used in the media when plating the cells. 1 ml of cell suspension was put into each well, then 2 mls of media. Cells were incubated overnight.
SOLUTIONS:
1. Stock solution: plain media (no serum or additives) + 20 mM HEPES.
2. IBMX solution: media/HEPES + lmM IBMX.
3. Assay solution: 90% stock solution + 10% IBMX solution.
Each well gets 3 mls of assay solution for preincubation and 3 mls for the assay. Each test condition is done in triplicate.
4. Drug solutions:
a) Basal assay solution + DMSO
b) Forskolin stimulation: 10 ~M final concentration.
c) Forskolin + competitor (melatonin): 10 ~M final concentration forskolin plus desired concentration of competitor (melatonin~.
REACTION:
All tests were done in triplicate at 37C. Plates with cells were kept in a shallow 37 C water bath throughout the reaction. Media was taken from the wells and 3 ml of preincubation media was added. After 10 min, that solution was removed and 3 mls of drug solution was added. After 10 min, the media was removed and reaction stopped with HCl. Samples set for at least an hour at room temperature. 1 ml from each dish was taken and put into a microfuge tube and spun to remove floating cells. After dilution to 1:100 for RIA, a radioimmuno-assay was done.

- ~ ~17~36~ -CT-231 lA/ BMMC7A

The following table sets forth selected Formula I compounds and intrinsic activity data which demonstrates their usefulness.

Functional Data of Selected Compounds of Formula I

Compound Name Ex. I.A.*
N-[2-(2,7-Dimethoxyfluoren-9-yl)ethyl] 5 1.14 propanamide N-[2-(2,7-Dimethoxyfluoren-9-yl)ethyl] 10 1.15 butanamide N-[2-(2,7-Dimethoxyfluoren-9-yl)ethyl] 7 1.02 cylopropane carboxamide *I.A. (Intrinsic Activity) = Emax (experimental compound)/Emax (melatonin) 10 Emax = maximal effect Reasonable variations, such as those which would occur to one having ordinary skill in the art, can be made herein without departing from the scope of the invention.

Claims (20)

1. A compound of Formula I or a pharmaceutically acceptable solvate thereof:

(I) wherein:
X= H, halogen, OH or OZ;
Z= C1-6 alkyl; -(CH2)m-CF3 (m=0-2); CD3 or (m'=1-3) n=1 or 2; and R= C1-6 alkyl, C3-6 cycloalkyl, C2-4 alkenyl, halogen substituted C1-6 alkyl, or C1-6 alkoxy substituted C1-6 alkyl.

2. The compound of claim 1 wherein at least one of X and ZO is methoxy.

3. The compound of claim 2 wherein R is methyl, ethyl, n-propyl, cyclopropyl, cyclopentyl, cyclobutyl or chloromethyl.

4. The compound of claim 3 selected from the group consisting of:
N-[2-(2,7-dimethoxyfluoren-9-yl)ethyl]cyclopentane carboxamide;
N-[2-(2-methoxyfluoren-9-yl)ethyl]butanamide;
N-[2-(2-methoxyfluoren-9-yl)ethyl] acetamide;
N-[2-(2,7-dimethoxyfluoren-9-yl)ethyl]butanamide;
N-[2-(2,7-dimethoxyfluoren-9-yl)ethyl]propanamide;
N-[2-(2,7-dimethoxyfluoren-9-yl)ethyl]cyclopropane carboxamide;
N-[2-(2,7-dimethoxyfluoren-9-yl)ethyl]acetamide;
N-[2-(2-ethoxy-7-methoxyfluoren-9-yl)ethyl]propanamide;
N-[2-(2-hydroxy-7-methoxyfluoren-9-yl)ethyl]propanamide;
N-[2-(2,7-dimethoxyfluoren-9-yl)ethyl]cyclobutane carboxamide; and N-[2-(2,7-dimethoxyfluoren-9-yl)ethyl]choroacetamide.

5. The compound of claim 2 wherein R is methoxymethyl, methylvinyl, vinyl, isopropyl, or t-butyl.

6. The compound of claim 5 selected from the group consisting of:
N-[2-(2,7-dimethoxyfluoren-9-yl)-ethyl]but-2-enamide;
N-[2-(2-methoxyfluoren-9-yl)ethyl]-2-propenamide;
N-[2-(2,7-dimethoxyfluoren-9-yl)ethyl]methoxyacetamide;
N-[2-(2,7-dimethoxyfluoren-9-yl)ethyl]-2-methyl propanamide; and N-[2-(2,7-dimethoxyfluoren-9-yl)ethyl]-2,2-dimethyl propanamide.

7. The compound of claim 2 wherein n=2.

8. The compound of claim 7 selected from the group consisting of:
N-[3-(2,7-dimethoxyfluoren-9-yl)prop-1-yl]propanamide;
N-[3-(2,7-dimethoxyfluoren-9-yl)prop-1-yl]butanamide;
N-[3-(2,7-dimethoxyfluoren-9-yl)prop-1-yl]acetamide;
N-[3-(2,7-dimethoxyfluoren-9-yl)prop-1-yl]cyclobutane carboxamide; and N-[3-(2,7-dimethoxyfluoren-9-yl)prop-1-yl]cyclopropane carboxamide.

9. The compound of claim 2 wherein X is F, -O(n-pentyl) or .

10. The compound of claim 1, N-[2-(2-methoxyfluoren-9-yl)ethyl]
butanamide.

11. The compound of claim 1, N-[2-(2,7-dimethoxyfluoren-9-yl)ethyl]
propanamide.

12. The compound of claim 1, N-[2-(2,7-dimethoxyfluoren-9-yl)ethyl]
cyclobutane carboxamide.

13. The compound of claim 1, N-[2-(2,7-dimethoxyfluoren-9-yl)ethyl]
choroacetamide.

14. The compound of claim 1, N-[2-(2,7-dimethoxyfluoren-9-yl)ethyl]
butanamide.

15. The compound of claim 1, N-[2-(2,7-dimethoxyfluoren-9-yl)ethyl]
acetamide.

16. The compound of claim 1, N-[2-(2,7-dimethoxyfluoren-9-yl)ethyl]
cyclopropane carboxamide.

17. The compound of claim 1 wherein X is OZ and Z is CH2CF3.

18. The compound of claim 1 wherein X is OZ and Z is CD3.

19. A method of treating a sleep disorder in a mammal in need of such treatment comprising administering to said mammal an effective amount of a compound of claim 1.

20. A pharmaceutical composition for treating sleep disorders comprising an effective amount of a compound of claim 1 and a suitable amount of a pharmaceutically acceptable carrier.