CA2156425A1 - Method and system for enhanced production of commercially important exoproteins in gram-positive bacteria - Google Patents

Method and system for enhanced production of commercially important exoproteins in gram-positive bacteria

Info

Publication number
CA2156425A1
CA2156425A1 CA002156425A CA2156425A CA2156425A1 CA 2156425 A1 CA2156425 A1 CA 2156425A1 CA 002156425 A CA002156425 A CA 002156425A CA 2156425 A CA2156425 A CA 2156425A CA 2156425 A1 CA2156425 A1 CA 2156425A1
Authority
CA
Canada
Prior art keywords
exoproteins
gram
positive bacteria
commercially important
enhanced production
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002156425A
Other languages
French (fr)
Other versions
CA2156425C (en
Inventor
Vesa Kontinen
Matti Sarvas
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority claimed from PCT/FI1994/000072 external-priority patent/WO1994019471A1/en
Publication of CA2156425A1 publication Critical patent/CA2156425A1/en
Application granted granted Critical
Publication of CA2156425C publication Critical patent/CA2156425C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention provides a method and expression system for enhancing secretion of hyperproduced homologous and heterologous exoproteins in gram-positive bacteria such as Bacillus sp. The method and system comprise overproduction of PrsA protein in a grampositive bacterial host capable of also overproducing at least one exoprotein of interest. Use of the method and system of the invention results in greatly enhanced secretion of the synthesized exoproteins into the growth medium. Once in the growth medium these secreted exoproteins can be recovered and purified in a straightforward manner.
CA002156425A 1993-02-26 1994-02-25 Method and system for enhanced production of commercially important exoproteins in gram-positive bacteria Expired - Lifetime CA2156425C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US2415493A 1993-02-26 1993-02-26
US08/024,154 1993-02-26
PCT/FI1994/000072 WO1994019471A1 (en) 1993-02-26 1994-02-25 Method and system for enhanced production of commercially important exoproteins in gram-positive bacteria

Publications (2)

Publication Number Publication Date
CA2156425A1 true CA2156425A1 (en) 1994-09-01
CA2156425C CA2156425C (en) 2007-05-01

Family

ID=38051318

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002156425A Expired - Lifetime CA2156425C (en) 1993-02-26 1994-02-25 Method and system for enhanced production of commercially important exoproteins in gram-positive bacteria

Country Status (1)

Country Link
CA (1) CA2156425C (en)

Also Published As

Publication number Publication date
CA2156425C (en) 2007-05-01

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Legal Events

Date Code Title Description
EEER Examination request
FZDC Discontinued application reinstated
MKEX Expiry

Effective date: 20140225