CA2151821A1 - Hydantoin and succinimide-substituted derivatives of spiroindanylcamphorsulfonyl oxytocin antagonists - Google Patents

Hydantoin and succinimide-substituted derivatives of spiroindanylcamphorsulfonyl oxytocin antagonists

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Publication number
CA2151821A1
CA2151821A1 CA002151821A CA2151821A CA2151821A1 CA 2151821 A1 CA2151821 A1 CA 2151821A1 CA 002151821 A CA002151821 A CA 002151821A CA 2151821 A CA2151821 A CA 2151821A CA 2151821 A1 CA2151821 A1 CA 2151821A1
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alkylamino
amino
imidazolyl
compound
mmol
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French (fr)
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Kevin Gilbert
Doug W. Hobbs
Daniel F. Veber
Peter D. Williams
Ben E. Evans
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Merck and Co Inc
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Individual
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    • C07D207/30—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having two double bonds between ring members or between ring members and non-ring members
    • C07D207/34—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having two double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
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    • C07D207/40—2,5-Pyrrolidine-diones
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • C07D295/22—Heterocyclic compounds containing polymethylene-imine rings with at least five ring members, 3-azabicyclo [3.2.2] nonane, piperazine, morpholine or thiomorpholine rings, having only hydrogen atoms directly attached to the ring carbon atoms with hetero atoms directly attached to ring nitrogen atoms
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    • C07D401/12—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings linked by a chain containing hetero atoms as chain links
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    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
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    • C07D405/06—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings linked by a carbon chain containing only aliphatic carbon atoms
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    • C07D471/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00 in which the condensed system contains two hetero rings
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    • C07F9/65583—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom containing at least two different or differently substituted hetero rings neither condensed among themselves nor condensed with a common carbocyclic ring or ring system each of the hetero rings containing nitrogen as ring hetero atom

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Abstract

Compounds of formula (I) where X is (a) or (b), R is Het, wherein Het is as defined in the description. These compounds are oxytocin and vasopressin antagonists useful in the treatment of preterm labor, dysmenorrhea, and for the stoppage of labor preparatory to cesarean delivery, timing of parturition, uterine hyperactivity, endometriosis, hypertension, congestive heart failure, hyponatremia and cognitive disorders.

Description

~o 94/14438 21~ 18 21 PCT/US93/12565 TITLE OF THE INVENTION
HYDANTOIN AND SUCCINIMIDE-SUBSTITUTED DERIVATIVES
OF SPIROINDANYLCAMPHORSULFONYL OXYTOCIN
ANTAGONISTS

FELD OF THE INVENTION
This application is a continl-~tion-in-part of application Serial No. 07/993,861, filed December 23, 1992, which is a continuation-in-part of application Serial No. 07/760,416, filed 0 September 16, 1991, now abandoned.
The present invention provides novel compounds, novel compositions, methods of their use and methods of their manufacture, such compounds generally pharmacologically useful as agents in obstetric and gynecologic therapy. The aforementioned pharmacologic 5 activities are useful in the treatment of m~mm~l~. More specifically, the compounds of the present invention can be used in the treatment of preterm labor, stopping labor preparatory to Cesarean delivery, and in the treatment of dysmenorrhea. At the present time, thelre is a need in the area of obstetric and gynecologic therapy for such agents.

BACKGROUND OF THE lNVENTION
In the field of obstetrics, one of the most important problems is the m~n~gement of preterm labor. A significant number of the pregnancies progressing past 20 weeks of gestation experience premature labor and delivery, which is a leading cause of neonatal morbidity and mortality. Despite major advances in neonatal care, retention of the fetus in utero is preferred in most instances.
Tocolytic (uterine-relaxing) agents that are currently in use include ~2-adrenergic agonists, magnesium sulfate and ethanol.
30 Ritodrine, the leading ,(~2-adrenergic agonist, causes a number of cardiovascular and metabolic side effects in the mother, including tachycardia, increased renin secretion, hyperglycemia (and reactive hypoglycemia in the infant). Other ,~2-adrenergic agonists, including terbutaline and albuterol have side effects similar to those of ritodrine.

WO 94/14438 . PCT/US93/1256~
2~ 8~

Magnesium sulfate at plasma concentrations above the therapeutic range of 4 to 8 mg/dL can cause inhibition of cardiac conduction and neuromuscular tr~n~mi~sion, respiratory depression and cardiac arrest, thus making this agent unsuitable when renal function is impaired.
5 Ethanol is as effective as ritodrine in preventing premature labor, but it does not produce a corresponding reduction in the incidence of fetal respiratory distress that ~(lmini~tration of ritodrine does.
It has been proposed that a selective oxytocin antagonist would be the ideal tocolytic agent. In the last few years, evidence has o accumulated to strongly suggest that the hormone oxytocin is the physiological initiator of labor in several m~mm~ n species including hllm~n~. Oxytocin is believed to exert this effect in part by directly contracting the uterine myometrium and in part by enhancing the synthesis and release of contractile prostaglandins from the uterine endometrium/decidua. These prostaglandins may, in addition, be important in the cervical ripening process. By these mech~ni~m~, the process of labor (term and preterm) is initiated by a heightened sensitivity of the uterus to oxytocin, resulting in part as a result of a well-documented increase in the number of oxytocin receptors in this tissue. This "up-regulation" of oxytocin receptors and enhanced uterine sensitivity appears to be due to trophic effects of rising plasma levels of estrogen towards term. By blocking oxytocin, one would block both the direct (contractile) and indirect (enhanced prostaglandin synthesis) effects of oxytocin on the uterus. A selective oxytocin blocker, or antagonist, would likely be more efficacious for treating preterm labor than current regimens. ~n addition, since oxytocin at term has major effects only on the uterus, such an oxytocin antagonizing compound would be expected to have few, if any, side effects.
The compounds of the present invention can also be useful in the treatment of dysmenorrhea. This condition is characterized by cyclic pain associated with menses during ovulatory cycles. The pain is thought to result from uterine contractions and ischemia, probably mediated by the effect of prostaglandins produced in the secretory endometrium. By blocking both the direct and indirect effects of WO 94114438 PCTtUS93112565 2151~
oxytocin on the uterus, a selective oxytocin antagonist can be more efficacious for treating dysmenorrhea then current regimens.
It is, therefore, a purpose of this invention to provide substances which more effectively antagonize the function of oxytocin in disease states in 7lnim~1~, preferably m~mm~l~, especially in hllm~n~. It is another purpose of this invention to prepare novel compounds which more selectively inhibit oxytocin. It is still another purpose of this invention to provide a method of antagonizing the functions of oxytocin when oxytocin activity is responsible for causing disease states in m~mm~l.c. It is also a purpose of this invention to develop a method of preventing or treating oxytocin-related disorders of preterm labor and dysmenorrhea by antagonizing oxytocin.
It has now been found that compounds of the present invention are antagonists of oxytocin and bind to the oxytocin receptor.
When the oxytocin receptor is bound by the compounds of the present invention, oxytocin is antagonized by being blocked from its receptor and thus being unable to exert its biologic or ph~rm~cologic effects.
These compounds are useful in the treatment and prevention of oxytocin-related disorders of ~nim~l~, preferably m~mm~l~ and especially hllm~n~. These disorders are primarily preterm labor and dysmenorrhea. The compounds would also find usefulness for stoppage of labor preparatory to Cesarean delivery.

2~S~82~
SUMMARY OF THE INVEN7rION
The compounds of the present invention and their ph~ ceutically acceptable salts and esters are those of the general structural formula:

H3C~ ~CH3 1~

R ~H--wherein xis ~ ~CH3 ~ ~ N

25 a is a single or double bond, Ris Het, wherein Het is a substituted saturated or unsaturated heterocyclic ring wherein said 3 substituents are independently one or more of Rl, R2, R3, Alk-R 1, Alk-R2, Alk-R3, -NHC(O)-Alk-R2R3, -NR5-Alk-R2R3 or Alk-R2R3;
where Alk is Cl lo alkyl and Rl, R2 and R3 are independently selected from the group consisting of hydrogen, halogen, C2 l0 alkenyl, methylene, Cl 10 alkoxycarbonyl, Cl 1o alkoxycarbonyl-WO 94/14438 P~T/US93/12565 , 5~i8~1 Cl lo alkylamino, Cl lo alkoxycarbonylamino, Cl lo alkylamino-Cl 1o alkylaminocarbonyl, Cl 1o alkylcarbonylamino, -S-R4, Cl lo alkylcarbonyloxy, Cl 10 alkylsulfonyl, Cl 10 alkylthio, amino, amino Cl 10 alkylcarbonylamino, amino Cl lo alkylamino, 5 carbonylamino, carbarnoyl, carboxyl C1-10 alkylamino, carboxyl, cyano, di-Cl lo alkylamino, di-cl-lo alkylamino-cl -10 alkylamino, di-Cl -10 alkylamino-C1 -10 alkylthio, di-Cl 10 alkylamino-Cl -10 alkylaminocarbonyl, guanidinyl, hydroxyl, hydroxyl Cl lo alkylamino, imidazolyl, imidazolyl amino, o imidazolyl Cl -10 alkylamino, imidazolyl Cl lo alkylaminocarbonyl, morpholinyl, thiomorpholinyl, dioxothiomorpholinyl, indolyl, oxo, oxiranyl, phenyl, piperidinylamino, piperazinyl, pyrrolidinyl, sulfonyl, tetrazolyl Cl 1o alkylcarbonylamino, tetrazolylaminocarbonyl, phosphoryl, phosphoryl C1 1o alkylamino and thiono;
R4 is selected from the group consisting of imidazolyl, C1 1o alkoxycarbonyl-C1 1o alkyl, di-Cl 1o alkylamino-C1 1o alkyl and C1 5 alkyl; and RS is selected from the group consisting of hydrogen and C~l-5 alkyl.

In one embodiment of the instant invention are compounds represented by the formula 1 ~<

S~ ~
R
wherein WO 94/14~38 PCT/US93/12565 2~$~
xis s o a is a single or double bond, Ris Het, wherein Het is a mono, di, tri or tetra substituted saturated or unsaturated S or 6 15 membered heterocyclic or 7 to 10 membered heterobicyclic ring cont~ining 1, 2 or 3 nitrogen atoms, wherein said substituents are independently one or more of Rl, R2, R3, Alk-Rl, Alk-R2, Alk-R3, -NHC(O)-Alk-R2R3, -NRS-Alk-R2R3 or Alk-R2R3; where Alk is Cl 1o alkyl and Rl, R2 and R3 are independently selected from the 20 group consisting of hydrogen, halogen, C2 10 aLkenyl, methylene, Cl 1o alkoxycarbonyl, Cl -10 alkoxycarbonyl-Cl -10 alkylamino, Cl -10 alkylcarbonylamino, Cl -10 alkylsulfonyl, -S-R4, amino, amino-Cl lo alkylcarbonylamino, amino Cl -10 alkylamino, carbamoyl, carboxyl Cl -10 alkylamino, carboxyl, cyano, 25 di-Cl lo alkylamino, di-Cl -10 alkylamino-Cl lo alkylamino, di-Cl -10 alkylamino-Cl -10 alkylthio, di-Cl 10 alkylamino-Cl lo alkylaminocarbonyl, guanidinyl, hydroxyl, hydroxyl-Cl lo alkylamino, imidazolyl, imidazolyl amino, imidazolyl-Cl lo alkylamino, morpholinyl, thiomorpholinyl, dioxothiomorpho-30 linyl, indolyl, oxo, oxiranyl, phenyl, piperidinylamino, piperazinyl,sulfonyl, phosphoryl, phosphoryl Cl lo alkylamino and thiono;
R4 is selected from the group consisting of imidazolyl, Cl lo alkoxycarbonyl-Cl lo alkyl, di-Cl lo alkylamino-Cl lo alkyl and Cl 5 alkyl; and RS is selected from the group consisting of hydrogen and Cl s alkyl.

~0 94/14438 PCT/US93/12565 2~ 2i In a second embodiment of the instant invention are compounds represented by the formula ~
[~ ~ H~C~CH3 SO~R H ~

or a pharmaceutically acceptable salt thereof, wherein a is a single or double bond, Ris Het, wherein Het is a mono, di, tri or tetra substituted saturated or unsatura~ed S or 6 20 membered heterocyclic ring cont~ining 1, or 2 nitrogen atoms that is bonded to said bicyclic ring at one of said heterocyclic ring's nitrogen atoms, wherein said substituents are independently one or more of Rl, R2, R3, Alk-Rl, Alk-R2, Alk-R3 or Alk-R2R3; and where Alk is Cl lo aLkyl and Rl, R2 and R3 are independently selected from the 25 group consisting of hydrogen, C2 l0 alkenyl, Cl 1o alkoxycarbonyl, Cl -10 aLkoxycarbonyl-Cl 10 alkylamino, Cl -lO alkoxycarbonylamino, Cl lo alkylamino-Cl 10 alkylaminocarbonyl, Cl lo aLkylcarbonyl-amino, Cl -lO alkylcarbonyloxy, Cl -lO alkylsulfonyl, Cl lo alkylthio, amino, amino-C1 10 alkylcarbonylamino, carbonylamino, 30 carboxyl Cl -lO alkylamino, carboxyl, carboxyl Cl -lO alkylamino, cyano, di-Cl -lO alkylamino, di-Cl lO alkylamino-Cl lo alkylaminocarbonyl, guanidinyl, hydroxyl, imidazolyl, imidazolyl Cl lO alkylaminocarbonyl, indolyl, oxo, phenyl, 2~5l8z~

piperidinylamino, piperizinyl, pyrrolidinyl, sulfonyl, tetrazolyl-Cl -10 alkylcarbonylamino, tetrazolylaminocarbonyl and thiono.

Salts encompassed within the term "pharmaceutically acceptable salts" refer to non-toxic salts of the compounds of this invention which are generally prepared by reacting the free base with a suitable organic or inorganic acid. Representative salts include the following salts:
Acetate Lactobionate Benzenesulfonate Laurate Benzoate Malate Bicarbonate Maleate Bisulfate Mandelate Bitartrate Mesylate Borate Methylbromide Bromide Methylnitrate Calcium Edetate Methylsulfate Camsylate Mucate 2 0 Carbonate Napsylate Chloride Nitrate Clav~ n~te N-methylgluc~mine Citrate Oxalate Dihydrochloride Pamoate (Embonate) Edetate P~lmit~te Edisylate. Pantothenate Estolate Phosphate/diphosphate Esylate Polygalacturonate Fumarate Salicylate 3 0 Gluceptate Stearate Gluconate Subacetate Gll-t~m~te Succinate Glycollylars~nil~te Tannate Hexylresorcinate Tartrate Hydrabamine Teoclate ~O 94/14438 ~ ~8. 2~7~ PC~/U593/12565 Hydrobromide Tosylate Hydrocloride Triethiodide Hydroxynaphthoate Valerate Iodide Isethionate Lactate The term "pharmacologically effective amount" shall mean that amount of a drug or ph~rm~ceutical agent that will elicit the o biological or medical response of a tissue, system, ~nim~l or hllm~n that is being sought by a researcher or clinician.
The term "alkyl" shall mean straight or branched chain alkanes of one to ten total carbon atoms or any number within this range.
The term "alkenyl" shall mean straight or branched chain alkenes, with one or more degrees of unsaturation at any position on the chain, of two to ten total carbon atoms, or any number within this range.
The term "aryl" shall mean phenyl.
The term "cycloalkyl" shall mean cyclic rings of alkanes, alkenes or alkynes with one or more degrees of unsaturation at any position of the ring, of three to eight total carbon atoms.
Whenever the terms "alkyl" or "aryl" or ei~her of their prefix roots appear in a name of a substituent (e.g., aralkoxyaryloxy) they shall be interpreted as including those limitations given above for "alkyl" and "aryl". Designated numbers of carbon atoms (e.g-, Cl 10) shall refer independently to the number of carbon atoms in an alkyl or cyclic alkyl moiety or to the alkyl portion of a larger substituent in which alkyl appears as its prefix root.
The term "heterocyclic" or "heterocycle," as used herein except where noted, represents a stable mono, di, tri or tetra-substituted 5- to 7- membered mono- or bicyclic or stable mono, di, tri or tetra-substituted 7- to 10-membered bicyclic heterocyclic ring system any ring of which may be saturated or lln~tllrated, and which consists of 2-I~1821 carbon atoms and from one to three heteroatoms selected from the group consisting of N and O. The heterocyclic ring may be attached at any heteroatom or carbon atom which results in the creation of a stable structure. Examples of such heterocyclic elements include piperidinyl, piperazinyl, azepinyl, pyrrolyl, dihydropyrrolyl, pyrrolidinyl, pyrazolyl, pyrazolidinyl, 4-piperidonyl, imidizolyl, imidazolinyl, imidazolidinyl, triazolyl, triazolinyl, triazolidinyl, morpholinyl, pyridyl, pyrazinyl, pyrimidinyl, pyridazinyl, oxazolyl, oxazolidinyl, isoxazolyl, isoxazolidinyl, quinuclidinyl, indolyl, quinolinyl, isoquinolinyl, benzimidazolyl, benzopyranyl, benzoxazolyl, oxadiazolyl, triazaspirodecane, pyrrolo-isoxazole, pyrrolo-pyrazole, and pyrrolo-pyrrole.
The term "oxo" shall refer to the substituent =O.
The term "thiono" shall refer to the substituent =S.
The term "phosphoryl" shall refer to the substitutent OPO(OH)2.
The term "oxiranyl" shall refer to the substituent /\
The term "dioxothiomorpholinyl" shall refer to the 25 substituent .
The term "halogen" shall include iodine, bromine, chlorine and fluorine.
The term "preterm labor" shall mean expulsion from the uterus of a viable infant before the normal end of gestation, or more 30 particularly, onset of labor with effacement and dilation of the cervix before the 37th week of gestation. It may or may not be associated with vaginal bleeding or rupture of the membranes.
The term "dysmenorrhea" shall mean painful menstruation.
The term "cesarean delivery" shall mean incision through the abdominal and uterine walls for delivery of a fetus.

~0 94/14438 PCT/US93/12565 .' t ~8~

- The term "substituted" shall be deemed to include multiple degrees of substitution by a named substitutent.
The ability of the compounds of the present invention to antagonize oxytocin makes these compounds useful as ph~rm~cologic 5 agents for m~mm~l~, especially for hl-m~n~, for the treatlment and prevention of disorders wherein oxytocin may be involved. Examples of such disorders include preterm labor and especially dysmenorrhea.
These compounds may also find usefulness for stoppage of labor preparatory to Cesarean delivery.
o Because of the known relationship of vasopressin to oxytocin, the compounds of the present invention are also useful as vasopressin antagonists. Vasopressin antagonists are useful in the treatment or prevention of disease states involving vasopressin disorders, including their use as diuretics and their use in congestive heart failure.
The compounds of the present invention can be a~1mini~tered in such oral dosage forms as tablets, capsules (each including timed release and sustained release formulations), pills, powders, granules, elixers, tinctures, suspensions, syrups and emulsions.
Likewise, they may also be ~lministered in intravenous (both bolus and infusion), intraperitoneal, subcutaneous or intramuscular form, all using forms well known to those of ordinary skill in the pharmaceutical arts.
An effective but non-toxic amount of the compound desired can be employed as a tocolytic agent.
The dosage regimen lltili7in~ the compounds of the present invention is selected in accordance with a variety of factors including type, species, age, weight, sex and medical condition of the patient; the severity of the condition to be treated; the route of ~lmini~tration; the renal and hepatic function of the patient; and the particular compound or salt thereof employed. An ordinarily skilled physician or veterinarian can readily determine and prescribe the effective amount of the drug required to prevent, counter or arrest the progress of the condition.

Oral dosages of the present invention, when used for the indicated effects, will range between about 0.3-6.0 gm/day orally.
Intravenously, the most preferred doses will range from 0.l to about l0 mg/ minute during a constant rate infusion. Advantageously, compounds of the present invention may be ~lmini~tered in a single daily dose, or the total daily dosage may be ~lmini~tered in divided doses of two, three or four times daily. Furthermore, preferred compounds for the present invention can be administered in intranasal form via topical use of suitable intranasal vehicles, or via transdermal routes, using those forms of transdermal skin patches well known to those of ordinary skill in that art. To be ~lminictered in the form of a transdermal delivery system, the dosage administration will, of course, be continuous rather than inte"~ t throughout the dosage regimen.
In the methods of the present invention, the compounds herein described in detail can form the active ingredient, and are typically a(lmini~tered in admixture with suitable pharmaceutical diluents, excipients or carriers (collectively referred to herein as "carrier" materials) suitably selected with respect to the intended form of ~lmini.~tration, that is, oral tablets, capsules, elixirs, syrups and the like, and consistent with conventional pharmaceutical practices.
For instance, for oral :~lmini~tration in the form of a tablet or capsule, the active drug component can be combined with an oral, non-toxic pharmaceutically acceptable inert carrier such as ethanol, glycerol, water and the like. Moreover, when desired or necessary, suitable binders, lubricants, disintegrating agents and coloring agents can also be incorporated into the mixture. Suitable binders include starch, gelatin, natural sugars such as glucose or beta-lactose, corn sweeteners, natural and synthetic gums such as acacia, tragac~n~ll or sodium alginate, carboxymethylcellulose, polyethylene glycol, waxes and the like. Lubricants used in these dosage forms include sodium oleate, sodium stearate, magnesium stearate, sodium benzoate, sodium acetate, sodium chloride and the like. Disintegrators include, without limitation, starch, methyl cellulose, agar, bentonite, x~nth~n gum and the like.

,~0 94114438 PCT/US93/12565 The compounds of the present invention can also be ~lmini~tered in the form of liposome delivery systems, such as small llnil~mellar vesicles, large unilamellar vesicles and multilamellar vesicles. Liposomes can be formed from a variety of phospholipids, such as cholesterol, stearyl~mine or phosphatidylcholines.
Compounds of the present invention may also be delivered by the use of monoclonal antibodies as individual carriers to which the compound molecules are coupled. The compounds of the present invention may also be coupled with soluble polymers as targetable drug carriers. Such polymers can include polyvinylpyrrolidone, pyran copolymer, polyhydroxypropylmethacrylamidephenol, polyhydroxyethylaspartamidephenol, or polyethyleneoxidepolylysine substituted with palmitoyl residues. Furthermore, the compounds of the present invention may be coupled to a class of biodegradable polymers useful in achieving controlled release of a drug, for example, polylactic acid, polepsilon caprolactone, polyhydroxy butyric acid, polyorthoesters, polyacetals, polydihydropyrans, polycyanoacrylates and cross-linked or amphipathic block copolymers of hydrogels.
The compounds of the present invention can be prepared readily according to the following reaction Schemes (in which all variables are as defined before) and Examples or modifications thereof using readily available starting materials, reagents and conventional synthesis procedures. In these reactions, it is also possible to make use of variants which are themselves known to those of ordinary skill in this art, but are not mentioned in greater detail.
The most preferred compounds of the invention are any or all of those specifically set forth in these examples. These compounds are not, however, to be construed as forming the only genus that is considered as the invention, and any combination of the compounds or their moieties may itself form a genus. The following examples further illustrate details for the preparation of the compounds of the present invention. Those skilled in the art will readily understand that known variations of the conditions and processes of the following preparative WO 94/14438 PCT/US93/1256~ ~

i5i~1 14 -procedures can be used to prepare these compounds. All temperatures are degrees Celsius unless noted otherwise.
Abbreviations used in the Examples are as follows:

TEA = triethylamine DIEA = diisopropylethyl~mine BOP = benzotriazolyloxytris(dimethylamino) phosphonium hexafluorophosphate THF = tetrahydrofuran 0 DMF = dimethylformamide LAH = lithium ~ lm hydride TFA = trifluoroacetic acid HPLC Method A = 15 min. linear gradient 95:5 A:B to 0:100 A:B
A = H2O cont~ining 0.1% by vol. TFA
B = CH3CN cont~inin~ 0.1% by vol. TFA
2.0 mL/min flow rate 12 cm C1g reverse phase column UV detection (215 nm) HPLC Method B = 20 min. linear gradient 90:10 A:B to 5:95 A:B
A = H2O cont~ining 0.1% by vol. phosphoric acid B = CH3CN containing 0.1% by vol. phosphoric acid 2.0 mL/min flow rate 12 cm C1g reverse phase column UV detection (215 nm) TLC was performed on 20 cm plates coated with silica gel (250 microns) from Analtech.

~0 94/14438 PCTIUS93/12565 ~ S
~';f' 1 ~ ~8?~

- EXAMPLE A

Endo-( 1 S)- 1 '(((2-amino-7 ,7-dimethylbicyclo(2.2. 1)- hept- 1 -yl)-methyl)-sulfonyl)spiro( 1 H-indan- 1.4'- piperidine) N
s~~

H N""~

Di-t-butyl dicarbonate (31g, 0.14 mole available from Aldrich) and bis(2-chloroethyl) amine hydrochloride (21.6g, 0.12 mole Aldrich) were combined in CH2Cl2 (250 ml) stirred at ambient temperature and treated with triethyl~mine (12.8 g, 0.127 mole) added 20 dropwise over 15 minutes. After 1 hour, another 1.5 ml of triethyl-amine was added. After a total of 2.5 hours, the mixture was poured onto a silica gel column packed with CH2Cl2:hexane (1:1), and eluted with CH2Cl2. The combined product fractions were evaporated to dryness vacuo to give N,N-bis(2-chloroethyl)-t-butyl-carbamate.
To a solution of indene (10.3 g, 89 mmole) in dry ~etrahydrofuran (THF, 18 ml) cooled in an ice bath and m~int~ined under a nitrogen blanket was added lithium bis(trimethylsilyl)amide (Aldrich, 177 ml of a l.OM solution in THF; 177 mmole) over 15 minutes. The mixture was stirred in the cold for 30 minutes, then added over 15 minutes to a solution of N,N-bis(2-chloroe~yl)-t-butylcarbamate (21.2 g, 88 mmole) stirred in an ice bath. The mixture was stirred for 2 hours in the cold and for 30 minlltes at ambient temperature under nitrogen, then evaporated in vacuo to a foam.
CH2C12 was added and the resulting mixture poured onto a silica gel column packed with 40% hexane in CH2cl2. The column was eluted - '2~S~

with 40% hexane in CH2C12 followed by CH2Cl2, and he product fractions were evaporated to dryness in vacuo to provide l'-(t-butyloxycarbonyl)-spiro(indene- 1 ,4'-piperidine).
1 '-(t-Butyloxycarbonyl)spiro(indene- 1,4'- piperidine) ( 16 g, 56 mmole) in ethyl acetate (250 ml) was stirred in an ice bath and saturated with HCl(g) for 30 minlltes. The mixture was evaporated to dryness. Ethyl acetate was added and removed in vacuo three times, and the residue was triturated with diethyl ether and filtered to provide spiro(lH-indene-1,4'-piperidine) hydrochloride. The free base was obtained by slurrying the hydrochloride in aqueous sodium bicarbonate solution and extracting with CH2Cl2. The organic layer was separated, dried over sodium sulfate, filtered, and evaporated to dryness in vacuo to provide spiro(lH-indene-1,4'piperidine.
Spiro(lH-indene-1,4'piperidine) (308 mg, 1.66 mmol) and (+)-10-camphorsulfonyl chloride (418 mg, 1.66 mmol) were combined in CH2Cl2 and treated with triethyl~mine (0.23 ml). The mixture was stirred at ambient temperature for 15 mimltes, then poured onto a silica gel column and eluted with 1:1 CH2Cl2:hexane. The product fractions were combined and evaporated to dryness in vacuo to provide (lS)-l'-(((7,7-dimethyl-2-oxobicicylo-(2.2. 1 )hept- 1 -yl)methyl)sulfonyl)spiro-(lH-indene- 1,4'-piperidine) as a solid which was recrystallized from petroleum ether and dried overnight in vacuo at ambient temperature.
( 1 S)- 1 '-(((7,7-dimethyl-2-oxobicyclo(2.2. 1 ) hept- 1 -yl)-methyl)sulfonyl)spiro(lH-indene-1,4'- piperidine) (30 g, 0.075 mole) in pyridine (500 mL) was heated in an oil bath to 70C (intemal).
Hydroxylamine hydrochloride (30 g) was added in three portions over ca. 20 minutes. After 2 hours, an additional 10 g of hydroxyl~mine hydrochloride was added (over 10 minutes). At 30, 40, and 50 minutes additional elapsed time, further 3 g lots of hydroxyl~mine hydrochloride were added. After another 30 minlltes, the mixture was poured into water (2 L) and extracted 3 times with ethyl acetate (300 mL portions). The organic layers were combined, washed with lN HCl (600 mL total), dried over sodium sulfate, filtered, and evaporated to dryness in vacuo. EtOH (abs, ca. 250 mL) was added to the resulting ~0 Y4/14438 ~S PCTIUS93/12565 ~c?~

thick syrup and the solution allowed to stand at ambient temperature overnight. The mixture was filtered and the filtrate boiled down to ca.
80 mL. After standing, the mixture was again filtered and boiled down to ca. 20 mL. After a third filtration, the filtered solids were combined to give (l S)~ (((7, 7-dimethyl-2-oximinobicyclo(2.2. 1 )hept- 1 -yl)-methyl) sulfonyl)spiro(lH-indene-1,4'-piperidine) (28 g).
Freshly prepared, activated Raney Nickel catalyst (ca. 30 g) in water was allowed to settle and the water decanted. Abs. ethanol (300 mL) was added, and the mixture swirled and again allowed to settle. The solvent was decanted. Two more wash- decant cycles with 150 mL of ethanol were similarly carried out. (lS)-1'-(((7,7-dimethyl-2-oximinobicyclo (2.2.1 )hept- 1 -yl)methyl)sulfonyl)-spiro( 1 H-indene- 1, 4'-piperidine) (30 g) was stirred in a mixture of abs. ethanol (450 mL) and 2-methoxyethanol (900 mL), nitrogen was bubbled through the suspension/solution, and the Raney Nickel catalyst was added. The mixture was hydrogenated under 50 psi overnight. TLC (9:1 CH2Cl2MeOH, silica gel) showed the reaction to be complete. The catalyst was removed by filtration, and the filtrate evaporated to dryness in vacuo. The crude solid (27 g) was divided into 7 g ba~ches, and each batch was dissolved in methylene chloride (ca. 200 mL) and flash chromatographed on silica (700 g in a 100 mm column, packed and eluted with 8% (v/v) methanol in methylene chloride), taking 200 mL
fractions. The exo isomer of the title amine was obtained in fractions ca. 5-7, and the desired endo isomer in fractions ca. 8-16. TLC was on silica, eluted with 8% methanol-methylene chloride, phosphomolybdic acid stain. The combined product fractions were evaporated to dryness to provide the title compound (4.5 g from each 7 g lot, ca. 18 g total) as a colorless solid.

WO 94/14438 PCT/US93/12~;65 ~S~ --EXAMPLE B

1 -((7,7-Dimethyl-2-oxo-bicyclo(2.2.1)heptan- 1 -yl)methanesulfonyl)-4-(2-methylphenyl)piperazine ~,CH3 L~N~ ~/

~ S2~

0~

To a stirred, 0C solution of l-(o-tolyl) piperazine hydrochloride (50.0 g; 235 mmol) and TEA (83 mL; 590 mmol) in chloroform (1000 mL) was added (+)-10-carnphorsulfonyl chloride (65.5 g; 260 mmol). The solution was stirred at 0C for 1 h and then at arnbient temperature for 3 h. The solution was extracted with 5%
20 aqueous HCl (2 x 500 mL), water (500 mL), and saturated aqueous NaHCO3 (2 x 500 mL). The orga~ic phase was dried (MgSO4), filtered, and the solvent was removed under reduced pressure. The resulting solid was recrystallized from methanol to give the title compound, mp 112-114C (69 g; 75%).
25 Analysis calculated for (C21H30N203S) C, 64.57; H, 7.74; N, 7.17 Found: C, 64.52; H, 7.68; N, 6.99 TLC: Rf 0.49 (75:25 hexane/e~yl acetate) HPLC (method A): retention time 10.33 min 30 FAB MS: m/z 391 (M+ + H) lH NMR (300 MHz, CDC13): ~ 7.2 (m, 2H), 7.0 (m, 2H), 3.45 (m, 4H), 3.40 (d, J=16 Hz, lH), 3.0 (m, 4H), 2.57 (m, lH), 2.40 (dt, Jd=14 Hz, Jt=3 Hz, lH), 2.30 (s, 3H), 2.10 (m, 2H), 1.96 (d, J=14 Hz, lH), 1.67 (m, lH), 1.44 (m, lH), 1.18 (s, 3H), 0.91 (s, 3H) ~0 94/14438 PCTIUS93/12565 ! ,,,~

- ( 1 S)- 1 '-(((7,7-dimethyl-2-endo-(4-nitrophenyloxycarbonylamino)-bicyclo-(2.2. 1 )-hept- 1 -yl)-methyl)- sulfonyl)spiro( 1 H-indane- 1,4'-5 piperidine) The product of Example A [3.47 mmol] and 4- nitrophenyl chloroformate [3.64 mmol] were combined in THF. The reaction mixture was treated with triethylamine [4.54 mmol] and allowed to stir for 2 hours. The reaction mixture was concentrated to dryness and the resulting residue was purified by a silica gel column, while eluting with 1% ethyl acetate in methylene chloride. The product fractions were combined and concentrated to dryness in vacuo. The title compound was obtained as a white solid from ether.

~Q
~N ~
SO,~

Oq~N~0 --NH

H
( 1 S)- 1 '-(((7,7-dimethyl-2-endo-(4-nitrophenyloxycarbonylamino)-bicyclo-(2.2. 1 )-hept- 1 -yl)-methyl))sulfonyl)spiro( 1 H-indane- 1 ,4'-piperidine) [1.80 mmol] and histidine methyl ester dihydrochloride [1.90 mmol] were combined in DMF. The reaction mixture was treated with triethyl~mine [5.90 mmol] and allowed to stir for 2 hours. The WO 94/14438 PCT/US93/12~;65 21~il8~

reaction mixture was concentrated to dryness and the resulting residue was dissolved in CH2C12. This CH2C12 solution was placed on a silica gel column and eluted with 2% methanol in CH2Cl2 and then with 95/5/0.5 of CH2Cl2/methanol/ammonium hydroxide. The product 5 fractiohs were combined and evaporated to dryness in vacuo. A white solid was obtained from ether. The resulting white solid [0.954 mmol]
and sodium hydride [0.45 mmol] were combined in ethanol and left to stir for 12 hours. The reaction mixture was concentrated to dryness and the resulting residue was dissolved in CH2C12. This solution was placed on a silica gel column and eluted with 95/5/0.5 of CH2Cl2/methanoV ammonium hydroxide. The product fractions were combined and evaporated to dryness in vacuo. The title compound was obtained as a white solid from ether and dried in vacuo. overnight.
m.p.: 146C-192C
NMR: Consistent with structure HPLC: >99% pure MS: M+H+=566.2 (FAB) CHN: Calc'd for C30H39N504S-0.05 C4H1oO-0.80 H2O;
C, 62.12; H, 7.10; N, 12.00.
Found: C, 62.10; H, 7.02; N, 12.01.

N
S~O~

0 Oq~N~f~O

~NH

The procedure of Example 2 was carried out using the product of Example 1 [0.197 mmol], triethylamine [0.54 mmol] and substituting gll-t~mine-t-butyl ester hydrochloride [0.217 mmol] for 20 histidinne methyl ester dihydrochloride. Chromatographic elution for column 1 was with 1% methanol in CH2Cl2 and then with 3% methanol in CH2C12. The title compound was obtained as a white solid from ether and dried in vacuo. overnight.
mp: 104-166C
25 NMR: Consistent with structure HPLE: >97% pure MS: M+H+=557.2 (FAB) CHN: Calc'd for C29H40N4oss-o.so C4HloO-0.10 H2O;
C, 62.51; H, 7.65; N, 9.41.
Found: C, 62.55; H, 7.36; N, 9.04.

WO 94/14438 PCT/US93/12~65 2~ 2-~

N~
SO~

H N~O
o~NH

o=~
O

The procedure of Example 2 was carried out using the product of Example 1 [0.215 mmol], triethyl~mine [0.55 mmol] and sub~ g L-methionine methyl ester [0.239 mmol] for histidine 20 methyl ester dihydrochloride. Chromatographic elution for column 1 was with 96/4/0.4 of CH2Cl2/methanol/ammonium hydroxide. For column 2, the elution was done with 5% methanol in CH2Cl2 and then with 95/5/0.5 of CH2Cl2/methanol/ammonium hydroxide. A white solid was obtained from ether. This white solid was dissolved in 25 methanol. This solution was treated with oxone [0.284 mmol], which had been dissolved in a small amount of water, and the mixture was stirred at ambient temperature for 4 hours. The reaction mixture was concentrated and the resulting residue was partitioned between ethyl acetate and sat'd sodium bicarbonate solution. The ethyl acetate layer 30 was dried over sodium sulfate, filtered, and the ~1ltrate was concentrated in vacuo. The residue was purified by a silica gel column, eluted with 2% methanol in CH2Cl2. The product fractions were combined and concentrated. The title compound was obtained as a white solid from ether, and dried in vacuo overnight.

94114438 PCT/US93/1256i m.p.: 134-209C
NMR: Consistent with structure - HPLC: >97% pure MS: M+H+=592 (FAB) CHN: Calc'd for C29H4lN3o6s2;
C, 58.86; H, 6.98; N, 7.10.
Found: C, 58.55; H, 6.59; N, 7.04.

~1 SO~

Oq~N~,~O
~NH

The procedure of Example 2 was carried ou~ using the product of Example 1 [0.366 mmol], triethyl~mine [0.83 mmol], and sub~LiLulillg N-a-Cbz-L-Lysine methyl ester [0.379 mmol] for histidine methyl ester dihydrochloride. Chromatographic elution for column 1 30 was with 95/5/0.5 of CH2Cl2/methanol/ammonium hydroxide. For column 2, the elution was done with 2% methanol in CH2Cl2. A white solid was obtained from ether. This white solid was combined with palladium hydroxide on carbon catalyst in absoute ethanol. The mixture was hydrogenated at 40 p.s.i. overnight. The reaction mixture was filtered and the filtrate was concentrated to dryness. The resulting WO 94/14438 215~ ~ 21; . PCTIUS93/12565 ~

residue was purified by a silica gel column, eluting with 92/8/0.8 of CH2C12/methanol/ammonium hydroxide. The product fractions were combined and evaporated to dryness. The title compound was obtained as a white solid from ether and was dried in vacuo, overnight.
m.p.: 99-158C
NMR: Consistent with structure HPLE: >94% pure MS: M+H+=557.3 (FAB) CHN: Calc'd for C30H44N404S-0.25 C4Hloo-H2o;
C, 64.11; H, 8.18; N, 9.65.
Found: C, 64.12; H, 8.01; N, 9.32.

SO~

H ~
o~NH

The procedure of Example 2 was carried out using the product of Example 1 [0.33 mmol], triethyl~mine [0.88 mmol], and 30 substituting L-leucine methyl ester [0.35 mmol] for histidine methyl ester dihydrochloride. Chromatographic elution for column 1 was with 95/5/0.5 of CH2C12tmethanol/ammonium hydroxide. For column 2, the elution was done with 1 % methanol in CH2C12. The title compound was obtained as a white solid from ether and dried in vacuo overnight.

~ 94/14438 21 S 1 8 21 PCT/US93/12565 - m.p.: 106-128C
NMR: Consistent with structure HPLE: >94% pure MS: M+H+=542.3 (FAB) CHN: Calc'd for C30H43N3O4S;
C, 66.51; H, 8.00; N, 7.76.
Found: C, 66.24; H, 8.10; N, 7.49.

~1 N ~
SO~

H ~
o~N

The procedure of Example 2 was carried out using the product of Example 1 [0.23 mmol], triethylamine [0.73 mmol], and substi~lting sarcosine ethyl ester [0.29 mmol] for histidine methyl ester 25 dihydrochloride. Chromatographic elution for column 1 was with 1%
ether in CH2Cl2 and then with 5% methanol in CH2Cl2. For column 2, elution was done with 25% ethyl acetate in hexane. The title compound was obtained as a white solid from ether and dried in vacuo, overnight.
m.p.: 89-152C
30 NMR: Consistent with structure HPLE: >96% pure MS: M+H+=500 (FAB) CHN: Calc'd for C27H37N304S-0.10 C4H100-0-40 H2C);
C, 63.99; H, 7.60; N, 8.17.
Found: C, 63.95; H, 7.37; N, 7.92.

WO 94/14438 21~1 8 2 f PCT/US93/12565 ~

s ~ .
N \~
SO~

lo H ~
o~NH

NH~

The procedure of Example 2 was carried out using the product of Example 1 [1.16 mmol], triethyl~mine [1.56 mmol], and substituting methyl(2-amino-3-(t-Boc-amino)) propanoate [1.27 mmol]
for histidine methyl ester dihydrochloride. Chromatographic elution 20 for column 1 was with 5% ether in CH2Cl2 and then with 3% methanol in CH2Cl2. For column 2, elution was done with 1 % methanol in CH2C12. The title compound was obtained as a white solid from ether and dried in vacuo, overnight.
m.p.: 104-176C
25 NMR: Consistent with structure HPLE: >97% pure MS: M+H+=615 (FAB) CHN: Calc'd for C32H46N406S-0.10 C4HloO-0.45 H20;
C, 61.73; H, 7.66; N, 8.89.
Found: C, 61.68; H, 7.66; N, 8.97.

94/14438 ~ 21 PCT/US93112565 ~N) ~/
/\
SO~

Oq~N~50 ~NH

0~< /
0~

The procedure for Example 2 was carried out using the product of Example 1 [0.27 mmol], triethyl~mine [0.76 mmol], and subslilulillg glutamic acid-a-methyl ester-oc-methyl ester-a-t-butylester 20 [0.308 mmol] for histidine methyl ester dihydrochloride. Chromato-graphic elution for column 1 was with 5% ether in CH2Cl2 and then with 5% methanol in CH2Cl2. For column 2, elution was done with 4%
methanol in CH2C12. The title compound was obtained as a white solid from ether and dried in vacuo. overnight.
2s m p: 94-117C
NMR: Consistent with structure HPLE: >93% pure MS: M~H+-614 ~FAB) CHN: Calc'd for C33H47N306S-0.10 C4HloO-0.50 H20;
C, 63.65; H, 7.84; N, 6.67.
Found: C, 63.68; H, 7.64; N, 6.67.

WO 94/14438 21~ 1 8 2 I PCT/US93/12565 ~

s ~
N \)~
SO,~

Oq~N ~f~O

~NH
~3 H

The procedure of Example 2 was carried out using the product of Example 1 [0.22 mmol], triethylamine [0.60 mmol], and substituting D-tryptophan methyl ester [0.24 mmol] for histidine methyl ester dihydrochloride. Chromatographic elution for column 1 was with 1 % ether in CH2C12 and then with 5% methanol in CH2Cl2. For column 2, elution was done with 4% methanol in CH2C12. The title compound was obtained as a white solid from ether and dried in vacuo~
Overnight.
m.p.: 111-176C
NMR: Consistent with structure HPLE: >92% pure MS: M+H+=615.2 (FAB) CHN: Calc'd for C35H42N404S-0.50 C4H1oO-0.85 H20;
C, 66.62; H, 7.29; N, 8.49.
Found: C, 66.64; H, 6.93; N, 8.12.

~ 94/14438 21 S l 8 21 PCT/US93112565 - EXAMPLE l 1 SO~

1 0 0~0 NH
The procedure of Example 12 was carried out using the product of Example 1 [1.38 mmol], triethyl~mine [3.40 mmol], and substituting glycine methyl ester hydrochloride [1.54 mmol] for histidine methyl ester dihydrochloride. Chromatographic elution for column 1 was with 1% e~er in CH2Cl2 and then with 4% methanol in CH2C12. For column 2, the elution was done with 99/1/0.1 of 20 CH2C12/methanol/ammonium hydroxide. The title compound was obtained as a white solid from ether and dried in vacuo, overnight.
m.p.: 230-239C
NMR: Consistent with structure HPLE: >92% pure 25 MS: M+H+=486 (FAB) CHN: Calc'd for C26H35N3O4S-0.10 C4H1oO-0.20 H2O;
C, 63.84; H, 7.39; N, 8.46.
Found: C, 63.77; H, 7.39; N, 8.50.

WO 94/14438 ` PCT/US93/12565 ~

N
SO,~

0~0 Succinic anhydride (12 mg, 0.12 mmols) and endo-(lS)-1'-(((2-amino-7,7-dimethylbicyclo-(2.2. 1 )-hept- 1 -yl)methyl)sulfonyl)-5 spiro(lH-indane-1,4'-piperidine) (50 mg, 0.12 mmols) were combined in a mixture of THF (1 mL) and methylene chloride (1 mL) and stirred at ambient temperature for eighteen hours. The solvents were removed under vacuum and the residue was treated with trifluoroacetic anhydride (1 mL) and toluene (2 mL), then heated to reflux for 15 20 minl-tes while the excess trifluoroacetic anhydride was allowed to boil out. The mixture was then cooled and evaporated to dryness in vacuo.
The residue was chromatographed on silica gel (8" column, 0.5" diam.), eluted with 0.5% (100 mL) followed by 1% (100 mL) methanol in methylene chloride. The product fractions were combined and 25 evaporated to dryness in vacuo. The residue was dissolved in ethyl acetate, diluted with hexane, and allowed to stand whereupon the title compound was deposited as a white solid. This material was filtered and dried in vacuo at 90 for eighteen hours.
m p: 228 5 229 5C
lH-NMR: Consistent with structure, ca. 0.1 mol of ethyl acetate and ca. 0.05 mol of hexane observed TLC: (2% MeOH in CH2Cl2) single component, Rf=0.66 MS: M+H+=485 (FAB) CHN: Calc'd for C27H36N2o4s-o.lo C4H8o2-o.o5 C6H14;

94/14438 g1 ~1 8 21 PCT/US93112565 C, 66.83; H, 7.59; N, 5.63.
Found:C, 66.62; H, 7.61; N, 5.51.

SO~

~0 NH
To a 0C solution of endo-(lS)-1'(((2-amino- 7,7-dimethylbicyclo (2.2.1)-hept- 1 -yl)-methyl)- sulfonyl)spiro(1 H-indan-1,4'-piperidine) (0.90 g; 2.2 mmol) and diisoprpylethylamine (DIEA) 20 (0-47 mL; 2.7 mmol) in CHCl3 (50 mL) was added iodoacetonitrile (0.38 grams; 2.3 mmol). The solution was stirred for 1 h at 0C and then for 18 h at ambient temperature. The mixture was extracted with aqueous NaHCO3 (2 x 25 mL), dried (MgSO4), filtered, and the solvent was removed under reduced pressure. The residue was purified by 2s pressurized silica gel column chromatography using 1 :3 ethyl acetate-hexanes as eluant (TLC Rf = 0.30 in 1 :3 ethyl acetate-hexanes; HPLC
retention time = 9.30 min). The purified cyanomethylated amine (0.80 g; 1.8 mmol) was dissolved in 2-methoxyethanol (15 mL) and to the stirred solution was added Raney nickel alloy (2.5 grams) followed by 30 6N NaOH solution (2.0 mL, 12 mmol). The mixture was heated to 80C on a steam bath and then stirred at ambient temperature for 14 h.
The catalyst was removed by filtration through Celite and washed with EtOAc. The filtrate solvents were removed under reduced pressure and the residue was taken up in CHCl3 (50 mL) and washed with water (2 x 25 mL). The organic phase was dried (MgSO4), filtered and WO 94/14438 PCT/US93/1256S ~

215i821 concentrated under reduced pressure. The residue was purified by pressurized silica gel column chromatography using 92:8:0.8 CHCl3:MeOH:NH40H as eluant (TLC Rf = 0.25 in 92:8:0.8 CHCl3:MeOH:NH40H; HPLC retention time = 7.20 min; FAB mass spectrum m/z = 446). The purified ~ mine (0.51 g; 1.1 mmol) was dissolved in CHCl3 and to the solution was added l,1'-carbonyldi-imidazole (0.19 g; 1.2 mmol). After the solution had been stirred for 1 h at ambient temperature, acetic acid (0.63 mL; 11 mmol) was added and the solution was refluxed for 6 h. The reaction was cooled and the solvent was removed under reduced pressure. The residue was dissolved in EtOAc (50 mL) and the solution was washed with 10%
aqueous citric acid (25 mL), water (25 mL), and aqueous NaHCO3 (25 mL). The organic phase was dried (MgSO4), filtered, and the solvent was removed under reduced pressure. The residue was purified by 5 pressurized silica gel column chromatography using 1:3 EtOAc:CHCl3 as eluant. The title compound was obtained as a white foam from CHCl3 (TLC Rf = 0.27 in 1:4 EtOAc:CHCl3;
HPLC retention time = 10.67 min;
FAB mass spectrum m/z=472; calc for C26H37N303S-0.70 CHC13: C, 57.76; H, 6.84; N, 7.75.
Found: C, 57.84; H, 6.82; N, 7.42;
lH NMR (CDCl3, 300 MHz) ~ 7.15-7.25 (m, 4H), 4.39 (ddd, J = 2.3, 5.3, 12.0 Hz, lH) 1.05 (s, 3H), 1.00 (s, 3H)).

HPLC conditions: 12 cm C1g reverse phase Vydac column; 15 min gradient 95:5 to 0:100 A:B (A = H2O cont~ining 0.1% TFA, B =
CH3CN cont~ining 0.1% TFA), flow rate = 2.0 mL/min, detection at 215 nm.

~ 94/14438 PCT/US93/12565 21 Sl 821 ~Q
t ~ ~/
N A

so~

H ~
~NH
l - so2 2-Amino-[1 -[[(2,3-dihydrospiro[lH-indene-1,4'- piperidin]-1 '-yl)sulfonyl]methy~]-7,7-dimethylbicyclo [2.2.1]hept-2-yl]-4-(methyl-sulfonyl)-but-l-ylamine (250 mg, 0.425 ~nmole) and thiocarbonyl-diimidazole (76 mg, 0.425 mmole) were combined with 500 mg of anhydrous cesium carbonate in 12 ml of dly N,N'-dimethylformamide at room temperature. The orange suspension was stirred for 2 hours, filtered, and concentrated under reduced pressure. The residue was partitioned between ethyl acetate (100 ml) and sodium bicarbonate solution. The phases were separated and the organic phase was washed with saturated sodium bicarbonate solution (3 X 40 ml) and brine, then dried (sodium sulfate) and concentrated. The crude product was (250 mg) was obtained as an oil which crystallized on standing in methanol:
NMR: Consistent with structure and verifies presence of solvent;
HPLC: > 97% pure at 214 nm;
FAB MS: 594 (M+ + H);
Elem. Anal. calc'd for C29H43N3O4S3-1.05 CH30H-0.25H20:
Calc'd: C, 57.10; H, 7.61; N, 6.65.
Found: C, 57.11; H, 7.21; N, 6.28.

,, WO 94/14438 PCT/US9311;!56~ ~
2i51821 1 -[1 -[[(2,3-Dihydrospiro[1 H-indene- 1,4'-piperidin] - 1 '-yl)sulfonyl]-methyl] -7,7-dimethylbicyclo[2.2.1]hept-2-yl] -2,5-dioxo-3-5 pyrrolidineacetic acid N
SO~

O~,, N ~O
L 1~

2-Carboxymethylsuccinic anhydride (3-carboxymethyl-tetrahydrofuran-2,5-dione) was prepared from tricarballylic acid as 20 described in J. Org. Chem. 46 2866 (1981). 2-Carboxymethylsuccinic anhydride (0.93g, 5.88 mmols) and endo-(lS)-1'-(((2-amino-7,7-dimethylbicyclo-(2.2.1)-hept-1 -yl)methyl)sulfonyl)- spiro(1 H-indene-1,4'-piperidine) (2.4g, 5.97 mmols) were combined in DMF (20 mL) and stirred at ambient temperature for eighteen hours. The DMF was 25 removed under vacuum and the residue was treated with lN HCl and extracted with methylene chloride. The methylene chloride layers were combined, dried over sodium sulfate, filtered, and evaporated to dryness vacuo. The residue was treated with toluene (100 mL) and trifluoroacetic anhydride (SmL), and the resulting mixture was heated 30 to reflux for 2-4 min while the excess trifluoroacetic anhydride was allowed to boil out. Reflux was continued for 10 min, and the mixture was then cooled and evaporated to dryness in vacuo. The residue was ch~omatographed on silica gel (10" column, 2"diam.), eluted with 200:10:1:1 CH2C12:MeOH:HoAc:H20. The product obtained by evaporation of the eluate was rechromatographed on silica gel twice, ~ 94/14438 PCT/US93/12565 21 Si ~

once eluted with lL each of 1000:10:1:1, 5()0:10:1:1, and 330:10:1:1 CH2C12:MeOH:HoAc:H2O, and the second time with 600: 10: 1: 1 of the same solvents. The combined product fractions were evaporated to dryness in vacuo~ treated wi~ ether and re-evaporated 3 tirnes, then 5 treated with hexane and evaporated to obtain the title compound as a solid which was dried in vacuo at 400C for eighteen hours.
M.P. 80- 100C (foam;indistinct) HPLC: 100%
H-NMR: Consistent with structure, ca. 0.05 mol of DMF and ca. 0.18 mol of hexane observed-TLC: (490:10:1:1 CH2C12:MeOH:HoAc:H2O) single component, Rf =
0.25.
M.S.: (FAB) M+H @ 543 Analysis for C29H3gN2O-0.05 C3H7NO-0.18 C6H14-0.3 H2O:
Calc'd: C, 64.00; H,7.37; N, 5.0 Found: C, 64.01; H,7.30; N, 5.12.

N
SO~

Oq~N~f~O
N
jN

WO 94/14438 PCT/US93/12565 ~
2i-S1182I' To a 0C stirred solution of p-nitrophenyl chloroformate (1.37 g; 6.8 mmol) in CHCl3 (100 mL) was added DIEA (1.18 ml; 12.4 mmol) and the product of Example A (2.5 g; 6.2 mmol). The solution was stirrred at 0C for 1 h and then at ambient temperature for 14 h.
The reaction mixture was concentrated under reduced pressure, the residue was dissolved in CHCl3 (100 mL) and washed with 5% aqueous HCl (2 x 50 mL) and aqueous NaHCO3 (100 mL). The organic phase was dried (MgSO4), filtered, and the solvent was removed under reduced pressure. The urethane was obtained as a white foam.
TLC: Rf 0.35 (1:3 EtOAc:hexanes) HPLC (method A): retention time 12.3 min To a 0C stirred solution of the p-nitrophenyl urethane (2.8 g; 5.0mmol) in DMF (20 mL) was added methyl 1-methyl-4-amino-4-piperidine carboxylate hydrochloride (1.04 gm, 5 mmol) and DIEA
(0.87 ml, 5 mrnol). The solution was stirred for 2 hours at ambient temperature. The reaction mixture was concentrated under reduced pressure, the residue was dissolved in CHC13 (100 mL) and washed with 5% aqueous HCl (2 x 50 mL) and 10 % aqueous Na2CO3 (5 x 100 mL).
The organic phase was dried (MgSO4), filtered, and the solvent was removed under reduced pressure. The urea was obtained as a foam which was crystallized from EtOAc ( 1.14 gm, 2 mmol).
Analysis calculated for (C31H44N404S)-1.85 H2O
C 60.61 H 8.22 N 8.84 Found: C 60.58 H 8.02 N 8.80 TLC: Rf 0.2 (95: 5: 0.5 CHCl3: MeOH: NH30H) HPLC (method A): retention time 9.17 min FAB MS: m/z 601 (M+ + H) To a 0C stirred solution of the urea (1.0 gm, 1.67 mmol) in MeOH ( 50 mL) was added in small portions NaH (dry powder) (0.125 gm, 5 mmol). The solution was stirred for 2 hours. The reaction mixture was neutralized with acetic acid and evaporated under reduced pressure. The residue was dissolved in CHCl3 (100 mL) and ~p 94/14438 PCTIUS93/12565 washed with 5% aqueous HCI (2 x 50 mL) and aqueous NaHC03 (100 mL). The organic phase was dried (MgS04), filtered, and the solvent was removed under reduced pressure. The title compound was obtained as a foam which was precipitated from EtOAc/ hexanes ( 0.260 gm, 0.5 mmol).
Analysis calculated for (C3 1H44N404S)-0.3 EtOAc C 64.98 H 7.86 N 9.41 Found: C 64.65 H 7.76 N 9.46 TLC: Rf 0.35 (95:5:0.5 CHC13:MeOH:NH40H) HPLC (method A): retention time 10.17 min FAB MS: m/z 569 (M+ + H) lH NMR (300 MHz, CDC13): d 7.15-7.25 (m, 4H), 5.8 (s, lH), 4.49 (m, lH), 2.3 (s, 3H), 1.02 (s, 3H), 0.97 (s, 3H) ~Q
~NJ ~
SO,~

~L~I

To a 0C solution of the unsubstituted hydantoin product of 30 Example 11 (1.50 g; 3.09 mmol) and 4-chloromethyl-1-(triphenyl)-methylimidazole (1.39 g; 3.87 mmol) in dry THF (60 mL) under an atmosphere of argon was added NaH (154 mg of a 60% suspension in mineral oil; 3.86 mmol). The mixture was stirred at 0C for 1 h, and then at ambient temperature for 24 h. Several drops of acetic acid were added and the mixture was concentrated under reduced pressure. The , WO 94/14438 PCT/US93/12~65 ~
8~

residue was dissolved in EtOAc (100 mL) and washed with aqueous NaHCO3 (2 x 50 mL). The organic phase was dried (MgSO4), filtered and concentrated under reduced pressure. The residue was purified by pressurized silica gel column chromatography using 1:1 EtOAc:CHCl3 as eluant. The product (1.40 g; 1.73 mmol) was heated in 10 mL of MeOH cont~ining 10 mL of 6N HCl at 60C for 6 h. The solvents were removed under reduced pressure and the residue was dissolved in CHCl3 (100 mL) and washed with aqueous NaHCO3 (2 x 50 mL). The organic phase was dried (MgSO4), ~lltered, and concentrated under 0 reduced pressure. The residue was purified by pressurized silica gel column chromatography using 95:5:0.5 CHCl3:MeOH:NH40H as eluant.
The purified product was dissolved in MeOH cont~inin~ 3 equivalents of 6 N HCl and the solvent was removed under reduced pressure. The residue was taken up in water-dioxane and lyophilized to give the HCl salt of title compound as a white powder.
Analysis calculated for (C30H39N5O4S)-2.05 HC1-0.55 H2O
C, 57.40; H, 6.53; N, 10.77 Found: C, 57.44; H, 6.53; N, 10.41 TLC: Rf 0.29 (95:5:0.5 CHC13:MeOH:NH40H) HPLC (method A): retention time 9.43 min FAB MS: m/z 566 (M+ + H) lH NMR (300 MHz, CDCl3): ~ 8.95 (s, lH), 7.40 (s, lH), 7.15-7.25 (m, 4H), 4.75 (m, 2H), 4.55 (m, lH), 1.03 (s, 3H), 0.97 (s, 3H) 2s ~ 94/14438 PCT/US93/12565 ~lsl82l ~Q
~N~ ~
SO,~
o9,~o --<_ OH

To a stirred solution of the hydantoin (150 mg; 0.309 mmol) in a mixture of 2:1 allyl bromide:tetrahydrofuran (30 mL) was added sodium hydride (12 mg; 60% dispersion in oil). The temperature was then increased to reflux. After 1 hr the solution was cooled, then concentrated. Purification by flash chromatography (5% methanol in 20 methylene chloride) provided the intermediate allyl derivative (158 mg).
The allyl hydantoin described above (105 mg; 0.20 mmol) was dissolved in a solution of 1:1 pyridine:toluene (12 mL). While stirring at room temperature, osmium tetraoxide (51 mg; 0.20 mmol) was added. After 8 hr 10 mL of a saturated aqueous solution of sodium bisulfite was added. The solution was allowed to stir for 1 hr, then diluted with ethyl acetate (50 mL). The ethyl acetate was separated, dried over sodium sulfate, then concentrated. Purification of the residue by flash chromatography (10% methanol in methylene chloride) 30 afforded the title compound (39 mg; 35%).
Analysis calculated for (C29H41N306S)-0.56 H2) C, 61.13, H, 7.45; N, 7.37 Found: C, 61.15; H, 7.55; N, 7.15 WO 94/14438 PCT/US93/12565_ 2151~2~ , HPLC: (Vydac C18 Column; gradient from 95/5 to 0/100 H20/CH3CN with 0.1% TFA. 15 min. gradient, flow rate = 1.5 ml/min.) Rt = 12.12 min. Purity = 96%
lHNMR: Consistent with structure FABMS: m/z = 560 (M+ + H) ~

N A
SO~

~`
o~,N~o To a stirred solution of N-methyliminodiacetic acid (220 mg; 1.50 mmol) in DMF (10 mL) was added DIEA (0.575 rnL; 3.30 mmol) and BOP (665 mg; 1.50 mmol). The mixture was stirred at 25 ambient temperature for 24 h, and then the product of Example A (500 mg; 1.24 mmol) was added. The mixture was strirred at ambient temperature for 24 h and then the solvent was removed under reduced pressure. The residue was dissolved in EtOAc (50 mL) and washed with 10% aqueous citric acid (20 mL) and water (10 mL). The organic 30 phase was dried (MgSO4), filtered, and the solvent was removed under reduced pressure. The residue was purified by pressurized silica gel column chromatography using a gradient elution of 5-10% MeOH-CHCl3. The purified monoacid, monoamide was obtained as a white foam.

~ 94/14438 21 ~1 8 21 PCT/US93/12565 - TLC: Rf 0.40 (90:10 CHCl3:MeOH) HPLC (method A): retention time 9.03 min FAB MS: m/z 532 (M+ + H) The purified monoacid, monoamide (150 mg; 0.282 mmol) was heated to reflux in a solution of THF (5 mL) and acetic anhydride (1 mL) for 14 h. The solvents were removed under reduced pressure and the residue was purified by pressurized silica gel column chromatography using 1 :4 EtOAc-hexanes as eluarlt. The title compound was obtained as a white foam from e~er.
Analysis calculàted for (C2gH39N304S)-0.2 ether-0.1 H2O
C, 65.23; H, 7.83; N, 7.92;
Found: C, 65.10; H, 7.99; N, 7.95;
TLC: Rf 0.29 (1:2 EtOAc:hexanes) HPLC (method A): retention time 10.57 min FAB MS: m/z 514 (M+ + H) lH NMR (300 MHz, CDCl3): ~ 7.10-7.25 (m, 4H), 5.20 (ddd, J = 2.2, 5.9, 12.1 Hz, lH), 3.40 (s, 3H), 2.37 (s, 3H), 1.06 (s, 3H), 0.95 (s, 3H) \/
N A
SO~
o ~
~N~"` H
3o N
~,0 O

(1 S)- 1 '-(((2-endo-Amino-7,7-dimethylbicyclo-(2.2.1) hept-l-yl)methyl) sulfonyl)spiro(lH-indane-1,4'-piperidine) (1.5 g, 3.7 WO 94/14438 . PCT/US93/12565 ~
2151~2~

mmole), tert-butylbromo acetate (0.8 g, 4.1 mmole), and crushed potassium carbonate (0.57 g, 4.1 mmole) were combined in 80 ml of absolute ethanol and heated at reflux for 12 hours. The reaction mixture was cooled, filtered, and rotoevaporated under reduced pressure. The 5 residual material was partitioned between ethyl acetate and water. The phases were separated and the organic layer was washed in succession with saturated sodium bicarbonate solution and brine, then dried (sodium sulfate), and concentrated to give a semi-solid. The crude product was crystallized from ethyl acetate to give 0.85 g of (lS)-l'-(((2-endo-tert-butyloxycarbonylmethylamino-7 ,7-dimethylbicyclo-(2.2.1 ) hept- 1 -yl)methyl)sulfonyl)spiro( 1 H-indane- 1,4'- piperidine).
Concentration of the mother liquors afforded an additional 0.99 g of material.
A solution of 40 ml of methylene chloride cont~inin~ 0.41 ml of triethyl~mine and 0.97 g of (lS)-1'-(((2-endo-tert-butyloxy-carbonylmethylamino-7,7-dimethylbicyclo(2.2. 1 ) hept- 1 -yl)methyl) sulfonyl) spiro(lH-indane-1,4'-piperidine) was stirred m~gnetically in an ice bath and treated in one portion with 0.24 ml of bromoacetyl-bromide. After 1 hour, an additional equivalent each of bromo-20 acetylbromide and triethylamine were added and the reaction mixturewas stirred at ambient temperature overnight. The reation mixture was diluted with methylene chloride and washed in succession with sodium bicarbonate solution, 10% citric acid solution, and brine. The dried extracts were concentrated and the residual material was flash 25 chromatographed on silica gel (15% ethyl acetate- hexane) to give 0.74 g of 2-tert-butyloxycarbonylmethylamino-N-[ 1 -[[(2,3-dihydro-spiro[ lH-indane- 1 ,4'-piperidin]- 1 '-yl)sulfonyl]methyl]-7,7- dimethyl-bicyclo[2.2. 1 ]hept-2-yl]-bromoacetamide.
A continuous stream of ammonia gas was passed for 10 minutes into an ice cold solution of methanol (32 ml) cont~ining 0.64 g (1.0 mmole) of 2-tert-butyloxycarbonylmethylamino-N-[1-[[(2,3-di-hydrospiroL 1 H-indane- 1 ,4'-piperidin] -1 '-yl)sulfonyl] - methyl] -7,7-dimethylbicyclo[2.2.1]hept-2-yl]-bromoacetamide. The reaction mixture was warmed to room temperature and stirred for 1 hour. All ,~ 94/14438 21 Sl 821 PCT/US93/12565 .;, `, `

volatile components were removed under reduced pressure to give a semi-solid which was partitioned between ethyl acetate and water. The organic phase was washed with water (3X) and brine, then dried (sodium sulfate) and concentrated. Triturate of the residual material 5 with ether gave 0.32 g of the title compound as an off-white solid: m.p.
>220C;
NMR: Consistent with structure:
HPLC: > 99% pure at 214 nm;
FAB MS: 500 (M+ + H);
o Elem. Analysis calculated for C27H37N304S-0.25 H2O:
C, 64.31; H, 7.51; N, 8.34.
Found: C, 64.32; H, 7.34; N, 8.14.

- \~
N ~
SO,~

~0~0 N
o~~/

To an ice cold suspension of trimethylsulfoxonium iodide (610 mg, 2.77 mmole) in 10 ml of dry tetrahydrofuran was added 1.8 30 ml of 1.6M n-butyllithium under nitrogen. After addition was complete the resulting reaction mixture was stirred at ambient temperature for 2 hours, re-cooled to 0C, and treated with a tetrahydrofuran solution (6 ml) cont~ining 620 mg (1.55 mmole) of (lS)-1'-(((7,7-dimethyl-2-oxobicyclo[2.2.1]hept- 1 -yl)methyl)sulfonyl)spiro(1 H-indene- 1,4'-piperidine). The reaction mixture was then stirred at ambient WO 94/14438 PCT/US93/12~;6~_ ~151821 temperature overnight. The reaction mixture was concentrated under reduced pressure to a volume of 6 ml and chromatographed on silica gel (hexane-ethyl acetate, 4:1) separating unreacted starting material and affording 390 mg of (lS)-1'-(((7,7-dimethyl-2-oxiranebicyclo-[2.2.1]hept- 1 -yl)methyl)sulfonyl) spiro-( l H-indene- 1,4'-piperidine).
To a suspension of 1.7 mmole of sodium hydride in 1.7 ml of dry N,N'-dimethylformamide was added 0.18 mmole of succinimide.
After stirring for 15 mimltes the reaction mixture became homogeneous and 70 mg (0.17 mmole) of (lS)-1'-(((7,7-dimethyl-2- oxiranebicyclo-[2.2.1]hept- 1 -yl)methyl)sulfonyl)spiro-(l H-indene- 1,4'-piperidine) was added. The reaction mixture was heated at 150C for 4 hours, then cooled to room temperature, and diluted with ethyl acetate. The organic phase was washed with water and brine, then dried, and concentrated to give 92 mg of crude product. Flash column chromatography on silica gel (30% ethyl acetate-hexane elution) of the crude reaction product afforded the title compound in analytically pure form as a white solid: m.p. l l l-115C;
NMR: Consistent with structure:
HPLC: > 99% pure at 214 nm;
FAB MS: 513 (M+ + H), 621 (M+ + thioglycerol);
Elem. Analysis calculated for C28H36N205S-0.75 H2O:
C, 63.90; H, 7.20; N, 5.32.
Found: C, 63.86; H, 7.14; N, 5.10.

~ 94/14438 PCT/US93112~65 21 $1 ~21 ~
lN~ ~/
SO~

1 o 0~0 N C N

To a 0C solution of the unsubstituted hydantoin product of Example 11 (1.50 g; 3.09 mmol) and iodoacetonitrile (1.03 g; 6.18 mmol) in dry THF (30 mL) under an atmosphere of argon was added NaH (185 mg of a 60% suspension in mineral oil; 4.64 mmol). The mixture was stirred at 0C for 1 h, and then at ambient temperature for 6 h~ The reaction was cooled to 0C and more iodoacetonitrile (0.52 g;
2 3.1 mmol) and NaH (124 mg of a 60% suspension in mineral oil; 3.1 mmol) were added. The mixture was stirred at 0C for 1 h, and then at ambient temperature for 14 h. Several drops of acetic acid were added and the dark brown mixtllre was concentrated under reduced pressure.
The residue was dissolved in EtOAc (100 mL) and washed with aqueous NaHCO3 (2 x 50 mL). The organic phase was dried (MgSO4), filtered and concentrated under reduced pressure. The residue was purified by pressurized silica gel column chromatography using 7:3 hexane:EtOAc as eluant, and then by preparative reverse phase HPLC using a water-acetonitrile gradient cont~inin~; 0.1% TFA. The title compound was obtained as a lyophilized powder.
Analysis calculated for (C28H36N404S)-0.35 TFA-0.25 H2O
C, 60.51; H, 6.44; N,10.22 Found: C, 60.57; H, 6.53; N, 9.85.
TLC: Rf 0.43 (3:2 hexane:EtOAc) WO 94/14438 PCT/US93112565 ~
21518~

HPLC (method A): retention time 11.39 min FAB MS: m/z 525 (M+ + H) lH NMR (300 MHz, CDCl3): ~ 7.1-7.3 (m, 4H), 4.56 (m, lH), 4.35 (AB quartet, J = 18 Hz, 2H), 3.95 (AB quartet, J = 16 Hz, 2H), 1.06 (s, 3H), 0.97 (s, 3H) N )~
SO~
~/
0~oO

HO 'OH
To a stirred solution of endo-(lS)-1'(((2- amino-7,7-dimethylbicyclo(2.2.1)-hept- 1 -yl)-methyl)-sulfonyl)spiro(l H-indan-1,4'-piperidine (526 mg; 1.31 mmol) in methylene chloride (20 mL) was added diacetyl-L-tartaric anhydride (312 mg; 1.44 mmol), followed by diisopropylethyl amine (0.251 mL; 1.44 mmol). After 18 hr the 25 solution was concentrated, then partitioned between ethyl acetate (200 mL) and 1 M HCl (200 mL). The ethyl acetate layer was washed with additional water (2 x 200 mL), then dried over sodium sulfate and concentrated. Partial purification by flash chromatography (10%
methanol in methylene chloride) afforded material that was dissolved in 30 methylene chloride (20 mL) and treated with thionyl chloride (0.096 mL; 1.31 mmol). After stirring at room temperature for 18 hr, the solution was concentrated. The intermediate diacetate was obtained by purification of the residue by flash chromatography (10% methanol in methylene chloride).

~94/14438 15~ 821 PCT/USg3/12565 The diacetate described above (1 g; 1.66 mmol) was dissolved in a solution of 3:1 tetrahydrofuran:water (40 mL) then cooled to 0C. A solution of 30% hydrogen peroxide (0.832 mL; 6.64 mmol) was added followed by lithium hydroxide (80 mg; 3.32 mmol).
5 After stiITing at 0C for 30 min the solution was concentrated. The title compound (492 mg; 73%) was obtained through purification of the residue by flash chromatography (10% methanol in methylene chloride).
Analysis calculated for (C27H36N206S)-0.35 H2O
C, 62.01; H, 7.07; N, 5.36 Found: C, 62.00; H, 6.86; N, 5.47 HPLC: (Vydac C18 Column; gradient from 95/5 to 0/100 H20/CH3CN with 0.1% TFA. 15 min. gradient, flow rate = 1.5 ml/min.) Rt= 12.5 min. Purity = 100%
lHNMR: Consistent with structure FABMS: m/z = 517 (M+ + H) [lR-[[(2,3-Dihydrospiro[lH-indene-1,4'-piperidin]-l '-yl)sulfonyl]-methyl]7,7-dimethylbicyclo[2.2.1]hept-2-endo-yl]-3-[2-propen- 1 -yl]-2,5-dioxo- 1 -imidazolidine N 1~>
OS~ O~,~O
- N~

WO 94/14438 . PCT/US93/12565 ~
, ~ , ;
21~1~21 To a stirred solution of [lR-[[(2,3-Dihydrospiro[lH-indene-1,4'-piperidin]-1 '-yl)sulfonyl]-methyl]7,7-dimethylbicyclo[2.2.1]hept-2-endo-yl]-2,5-dioxo-1-imidazolidine (150 mg; 0.309 mmol) in tetrahydrofuran (20 mL) was added allyl bromide (27 ~lL; 0.309 5 mmol), followed by sodium hydride (12 mg; 60% dispersion in oil).
The temperature was increased to reflux. After 4 h the solution was cooled then concentrated. Puri~lcation by flash chromatography (5%
methanol in methylene chloride) provided the title compound as a white solid (81 mg).
lHN~R: consistent with structure.
M.P.: 101-104C
HPLC: Rt = 14.7 min; 95%
FABMS: M+ 1 at526 Analysis calculated for C29H39N304S + 0.05 CH2C12 + 0.40 H2O
C, 64.95; H, 7.49; N, 7.82 Found: C, 64.93; H, 7.48; N, 7.43 20 [lR-[[(2,3-Dihydrospiro[lH-indene-1,4'-piperidin]-1'-yl)sulfonyl]-methyl]7,7-dimethylbicyclo[2.2.1]hept-2-endo-yl]-3-[2-hydroxy-3-[1,1-dimethylaminol-propan- 1 -yll- 2 5-dioxo- 1 -imidazolidine N
OS` o~N~O OH
N~,N

To a stirred solution of [lR-[[(2,3-Dihydrospiro[lH-indene-1,4'-piperidin]-1'-yl)sulfonyl]-methyl]7,7-dimethylbicyclo[2.2.1]hept-2-endo-yl]- 2,5-dioxo-1-imidazolidine (164 mg; 0.338 mmol) in tetra-hydrofuran (2 mL) was added bromoepihydrin (1 mL), followed by sodium hydride (12 mg; 60% dispersion in oil). 'lhe mixture was then heated to reflux. After 6 hours, the mixture was cooled and concen-trated. Purification by flash chromatography (30% ethyl acetate in petroleum ether as eluent) afforded [lR-[[(2,3-Dihydrospiro[lH-indene-1,4'-piperidin]- 1 '-yl)sulfonyl]-methyl]7,7-dimethylbicyclo[2.2.1]hept-2-endo-yl]-3-[2,3 oxirane-1-propenyl]- 2,5-dioxo-1-imidazolidine as a white foam (141 mg).
To a solution of [lR-[[(2,3-Dihydrospiro[lH-indene-1,4'-piperidin]- 1 '-yl)sulfonyl]-methyl]7,7-dimethylbicyclo[2.2.1]hept-2-endo-yl]-3-[2,3 oxirane-1-propenyl]- 2,5-dioxo-1-imidazolidine (73 mg;
0.135 mmol) in absolute ethanol (2 mL) was added dimethyl~mine hydrochloride (55 mg; 0.68 mmol) and diisopropylethyl~mine ((47 ,uL).
After 6 hours at reflux, the solution was cooled and concentrated.
Purification by preparative HPLC afforded the title compound (41 mg).
lHNMR: consistent with structure.
M.P.: 93-97C
HPLC: Rt = 11.63 min; 99%
FABMS: M + 1 at 587 Analysis calculated for C31 H46N4O5S + 0.65 CH2C12 + 0.20 H2O
2 C, 58.88; H, 7.45; N, 8.08 Found: C, 58.90; H, 7.46; N, 8.53 2s WO 94/14438 PCT/US93/12565 3~1~
215182i - so -[1 R-[ [(2,3 -Dihydrospiro [1 H-indene- 1,4'-piperidin] - 1 '-yl)sulfonyl] -methyl]7,7-dimethylbicyclo[2.2.1]hept-2-endo-yl] -3-[2,5-dioxo-3S-[4-5 aminopropylamidoll - 1 -succinimide ~N ~ >

~ J~--NH2 To a solution of endo-[lS]-1'[[[2-amino-7,7-dimethyl-bicyclo[2.2.1] -hept- 1 yl] -methyl] -sulfonyl] spiro[1 H-indan- 1 -4'-piperidine] (lg, 2.48 mmol) in methylene chloride (75 mL) was added Boc-(L)-Aspartic acid ,~-methyl ester (675 mg, 2.72 mmol), 20 hydroxybenzotriazole (436 mg, 3.22 mmol), and 1-(3-dimethyl-aminopropyl)-3-ethylcarbodiimide hydrochloride (618 mg, 3.22 mmol).
After 4 hours the solution was concentrated, then partitioned between ethyl acetate and lM NaOH (75 mL each). The organic layer was washed with lM HCl, and brine (75 mL each) then dried over Na2SO4.
25 The solution was filtered and concentrated. Purification by flash chromatography (40% ethyl acetate in petroleum ether as eluent) gave an amide ester intermediate as white foam (l.lSg).
The foam was dissolved in dry tetrahydrofuran (100 mL) under nitrogen atmosphere, then cooled to -78 C. Lithium hexamethyldisilylazide (3.64 mL, lM solution in tetrahydrofuran) was added dropwise. After 4 hours, a saturated solution of ammonium chloride was added, and the reaction mixture was allowed to warm to room temperature. The mixture was partitioned between ethyl acetate and water (75 mL each). The ethyl acetate layer was dried over sodium 94/14438 ~l PCT/US93/12565 sulfate, then concentrated. Purification by flash chromatography (gradient from 15% to 20% ethyl acetate in petroleum ether as eluent) afforded a protected aminosuccinimide intermediate as a white foam (l.lg).
To a solution of the aminosuccinimide (1.46 g, 2.43 mmol) in ethyl acetate (50 mL) was indroduced a stream of HCI gas. After 15 min. the HCl was removed and the solution was washed with lM sodium carbonate. The ethyl acetate layer was dried over sodium sulfate, filtered, and concentrated. Purification by flash chromatography (a gradient from 2% to 10% methanol in methylene chloride as eluent) afforded an intermediate unprotected aminosuccinimide as a white foam (1.12g).
To a solution of the unprotected aminosuccinimide (90 mg, 0.18 mmol) in methylene chloride (15 mL) was added Boc-b-alanine (51 mg, 0.27 mmol), hydroxybenzotriazole (37 mg, 0.27 mmol), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (52 mg, 0.27 mmol), and diisopropylethyl~mine (47~1L, 0.27 mmol). After stirring at room temperature for 6 hours the mixture was concentrated, then partitioned between ethyl acetate and lM HCI (200 mL each). The ethyl acetate layer was dried over sodium sulfate, then filtered and concentrated. Purification by preparative HPLC afforded an adduct which was dissolved in methylene chloride (20 mL) and treated with trifluoroacetic acid (8 mL). After 1 hour, the solution was concentrated, then the product was purified by preparative HPLC to give the title compound (71 mg).
lHNMR: consistent with structure.
HPLC: Rt = 12.2 min; 97%
FABMS: M+latS71 Analysis calculated for C30H42N4OsS + 2.5 trifluoroacetic acid C, 46.89; H, 5.47; N, 6.83 Found: C, 46.91; H, 5.30; N, 6.77 -WO 94/14438 ~2~-- PCT/US93/12565~

, [ 1 R-[[(2,3-Dihydrospiro[ lH-indene- 1 ,4'-piperidin]- 1 '-yl)sulfonyl] -methyl]7,7-dimethylbicyclo[2.2. 1 ]hept-2-endo-yl]-3 endo-[2,5-dioxo-3S-5 amino-r4-piperidinylll-1-succinimide lo ~N
0,~ o~O
H~NH

To a solution of [lR-[[(2,3-Dihydrospiro[lH-indene-1,4'-piperidin]- 1 '-yl)sulfonyl] -methyl]7,7-dimethylbicyclo[2.2. 1 ]hept-2-endo-yl]-3 endo-[2,5-dioxo-[3S-amino]]-1-succinimide (161 mg, 0.32 20 mmol) in methanol (15 mL) was added Boc-4-piperidinone (77 mg, 0.39 mmol), and sodium cyanoborohydride (61 mg, 0.96 mmol). After stirring at room temperature for 4 hours, the mixture was concentrated and purified by flash chromatography (5% methanol in methylene chloride as eluent).
The residue was redissolved in methylene chloride (10 mL), then treated with trifluoroacetic acid (5 mL). After 2 hours the solution was concentrated. Purification by flash chromatography (10%
methanol in methylene chloride as eluent) afforded the title compound (91 mg).
lHNMR: consistent with structure.
HPLC: Rt = 13.2 min; 98%
FABMS: M + 1 at 583 ~p 94/14438 PCT/US93112565 ~1 Sl 821 Analysis calculated for ('32H46N404S + 1.5 trifluoroacetic acid +
1.5 H20 C, 53.84; H, 6.52; N, 7.18 Found: C, 53.85; H, 6.69; N, 6.79 E~AMPLE 28 [ lR-[[4-(2-methylphenyl)piperazin- 1 -yl)sulfonyl]-methyl]7,7-dimethylbicyclo[2.2.1]hept-2-endo-yl]-3-[2R-hydroxy-3-[piperazin- 1 -yll-propan- 1 -yll-2.5-dioxo- 1 -imidazolidine CH

~ ~OH ~NH
N ,N~J

To a stirred solution of [lR-[[4-(2-methylphenyl)piperazin-1 -yl)sulfonyl]-methyl]7,7-dimethylbicyclo[2.2.1]hept-2-endo-yl]- 2,5-dioxo-1-imidazolidine (120 mg, 0.253 mmol) in dry tetrahydrofuran (15 mL) was added 2R-(-)-glycidyl tosylate (288 mg, 1.26 mmol), 25 followed by sodium hydride (60% dispersion in oil). The temperature was increased to reflux. After 2 hours the mixture was cooled then concentrated. Purification by preparative TLC (40% ethyl acetate in petroleum ether as eluent) afforded [lR-[[4-(2-methylphenyl)piperazin-1 -yl)sulfonyl]-methyl]7,7-dimethylbicyclo[2.2.1]hept-2-endo-yl]-3-[2S,3 30 oxirane-1-propenyl]- 2,5-dioxo-1-imidazolidine as a white foam (112 mg).
To a solution of [lR-[[4-(2-methylphenyl)piperazin-1-yl)sulfonyl]-methyl]7,7-dimethylbicyclo[2.2.1]hept-2-endo-yl]-3-[2S,3 oxirane-l-propenyl]- 2,5-dioxo-1-imidazolidine (66 mg, 0.12 mrnol) in absolute ethanol (20 mL) was added piperazine (50 mg, 0.58 mmol).

215~

The temperature was increased to reflux. After 2 hours the solution was cooled and concentrated. Purification by flash chromatography (85:15:1 methylene chloride:methanol:ammonium hydroxide as eluent) afforded the title compound (19 mg).
5 1HNMR: consistent with structure.
HPLC: Rt = 10.4 min; 95%
FABMS: M + 1 at 517 Analysis calculated for C31H4gN6O5S + 0.15 hexanes + 1.2 H2O
C, 58.82; H, 8.12; N, 12.90 Found: C, 58.84; H, 7.76; N, 12.54 N ~>

OS~ o~O

To a solution of endo-(lS)-1'(((2-amino-7,7-dimethyl-bicyclo(2.2.1)-hept- 1 -yl)-methyl)-sulfonyl)spiro( l H-indan- 1,4'-piperidine) (3 g, 7.45 mmol) in methylene chloride (150 mL) was added maleic anhydride (876 mg, 8.94 mmol). After stirring for 2 h at room 25 temperature, the mixture was concentrated, then redissolved in acetic anhydride (100 mL). Sodium acetate was added (611 mg, 7.45 mmol), then the temperature was increased to reflux. After 48 h, the mixture was cooled to room temperature, then concentrated. Flash chromatography using 50% ethyl acetate in petroleum ether afforded 30 1.5 g of the title compound as a white foam.

WO 94/14438 . PCT/US93/12565 21~18~1...... t lHNMR: consistent with structure.
HPLC: method A, Rt = 14.86 min; 96%
- FABMS: M + 1 at 483 Analysis calculated for C27H34N2O4S + 0.35 dioxane C, 66.43; H, 7.22; N, 5.46 Found: C, 66.45; H, 7.25; N, 5.23 ~N ~ N 5~o ~CO2Et O~N~--COzEt To a solution of the product of Example 29 (84 mg, 0.17 20 mmol) in 1:1 methylene chloride:diethyl ether (15 mL) was added chloroximidoacetate (32 mg, 0.21 mmol), followed by diisopropyl-ethylamine (37 uL). The mixture was allowed to stir at room temperature for 4 h at which time additional chloroximidoacetate was added (32 mg). After 18 h, the mixture was concentrated and the 25 residue was applied to preparative TLC plates. Two products were obtained as white solids, in 40% overall yield.
lHNMR: consistent with structure.
HPLC: method A, Rt = 14 73 min; 100%
FABMS: M + 1 at 598 30 Analysis calculated for C31H3gN3O7S + 0.55 chloroform C, 57.21; H, 5.87; N, 6.34 Found: C, 57.18; H, 5.88; N, 6.41 1HNMR: consistent with structure.
HPLC: method A, Rt = 14.99 min; 100%

WO 94/14438 PCT/US93/12~6~
2i~

FABMS: M + 1 at 598 Analysis calculated for C31H39N307S + 0.20 chloroform C, 60.29; H, 6.36; N, 6.76 Found: C, 60.27; H, 6.29; N, 6.72 ~N ~) ~ ~

Bn To a solution of the product of example 29 (109 mg, 0.23 20 mmol) in acetonitrile (10 mL) was added silver iodide (57 mg, 0.46 mmol), followed by a solution of N-benzyl-N-(trimethylsilylmethyl)-aminoacetonitrile (111 uL, 0.46 mmol) in acetonitrile (10 mL). After stirring at room temperature in the dark for 18 h, the mixture was filtered, then concentrated. The title compound was obtained in 60%
25 yield by preparative TLC using 25% ethyl acetate in petroleum ether as eluent.
1HNMR: consistent with structure.
HPLC: method A, Rt = 12.21 min; 97%
TLC: Rf = 0.2 (20% ethyl acetate in petroleum ether) 30 Analysis calculated for C36H45N304S + 0.45 water C, 69.30; H, 7.42; N, 6.73 Found: C, 69.34; H, 7.39; N, 7.02 r 5 7 N~ ~\
OS~ o~70 ~;~
H

To a solution of the product of Example 31 (50 mg, 0.081 15 mmol) in ethanol (15 mL) was added palladium black (5 mg), followed by acetic acid (1 drop). After stirring at room temperature under an atmosphere of hydrogen for 18 h, the mixture was filtered then concentrated. The title compound was obtained by preparative HPLC
(20 mg).
lHNMR: consistent with structure.
HPLC: method A, Rt = 11.97 min; 97%
TLC: Rf = 0.5 (10% methanol in methylene chloride) Analysis calculated for C29H39N304S + 1.25 trifluoroacetic acid +
0.60 toluene.
C, 59.26; H, 6.28; N, 5.81 Found: C, 59.27; H, 6.26; N, 5.86 WO 94/14438 . PCT/US93/1256~
215182~

~N~
OSO 0~0 o "N

To a solution of the product of Example 29 (660 mg, 1.37 mmol) in 1:1 tetrahydrofuran:diethyl ether (200 mL) was added an ethereal solution of diazomethane (approximately 5 eq.). After stirring at room temperature for 1 h, acetic acid (2 drops) was added, then the mixture was concentrated. The title compound (717 mg) was obtained as a 3:1 mixture of diastereomers by flash chromatography using 40%
ethyl acetate in petroleum ether as eluent.
1HNMR: consistent with structure.
HPLC: method A, Rt = 13.48 min (major isomer) TLC: Rf = 0.5 (40% ethyl acetate in petroleum ether) FABMS: M + 1 at 525 Analysis calculated for C2gH36N4O4S + 0.3 ethyl acetate C, 63.93; H, 7.02; N, 10.17 Found: C, 63.94; H, 7.09; N, 10.13 . , ~5~821 N~
OSO 0~0 ,NH

To a solution of the product of Example 33 ~40 mg, 0.08 15 mmol) in 9:1 methanol:acetic acid (20 mL) was added zinc dust (10 eq).
After stirring at room temperature for 4 h, the mixture was ~lltered and concentrated. The title compound (15 mg) was obtained through purification by flash chromatography (10% methanol in methylene chloride as eluent).
20 1HNMR: consistent with structure.
HPLC: method A, Rt = 11.03 FABMS: M + 1 at 527 Analysis calculated for C2gH3gN4O4S + 0.5 water + 0.25 hexanes C, 63.59; H, 7.69; N, 10.05 25 Found: C, 63.61; H, 7.31; N, 9.79 N ~ N
,S~ o~o and O O ~

(HO)20po opo(OH)2 (HO)20PO OH

To a solution of the product of Fx~mple 23 (1.03 g, 2 mmol) in dry tetrahydrofuran(100 mL) was added diethylamino 15 dibenzylphosphoramidite (1.78 g, 04 mmol), followed by tetrazole (280 mg, 4 mmol). After 2 h, the solution was cooled to -40C, then m-chloroperbenzoic acid (1 g, 4 mmol) in methylene chloride (12 mL) was added dropwise. The solution was allowed to warm to 5C. After 18 h, the mixture was partitioned between aqueous sodium bisulfite and 20 methylene chloride. The methylene chloride layer was dried over sodium sulfate, then concentrated. Flash chromatography (3% methanol in methylene chloride as eluent) allowed the separation of two phosphorylated intermediates (mono and diphosphorylated adducts) which were hydrogenated separately.
Each of the two phosphorylated intermediates was dissolved in ethanol. Palladium on carbon (10%) was added, then the mixtures were placed under hydrogen atmosphere. After 18 h, the mixtures were filtered and concentrated. The product phosphates were purified by preparative HPLC.

mono phosphate:
lHNMR: consistent with structure.
HPLC: method B, Rt= 11.27 min.
FABMS: M+ 1 at597 WO 94/14438 ~ , PCT/US93112565 Analysis calculated for C27H37N209SlPl + 0.65 trifluoroacetic acid + 0.70 dioxane C, 51.00; H, 5.95; N, 3.83 Found: C, 51.05; H, 6.35; N, 4.21 di phosphate:
lHNMR: consistent with structure.
HPLC: Rt = 10.5 min.
FABMS: M + 1 at 677 Analysis calculated for C27H3gN2O12P2Sl + 1.50 dioxane C, 49.00; H, 6.23; N, 3.46 Found: C, 48.97; H, 6.24; N, 3.43 ¢~N ~>
0'0 ~

To a solution of endo-[lS]-1'[[[2-amino-7,7-dimethyl-bicyclo[2.2.1]-hept- 1 yl]-methyl] -sulfonyl]spiro[ l H-indan- 1 -4'-piperidine] (4 g, 0.01 mol) in methylene chloride (20 mL) was added Boc-(D)-Aspartic acid beta-benzyl ester (3.32 g, 0.012 mol), 30 hydroxybenzotriazole (1.62 g, 0.012 mol), and 1-(3-dimethylamino-propyl)-3-ethylcarbodiimide hydrochloride (2.3 g, 0.012 mmol). After 18 hours the solution was concentrated, then partitioned between ethyl acetate and lM NaOH (150 mL each). The organic layer was washed with lM HCl, and brine (150 mL each) then dried over Na2SO4. The solution was filtered then concentrated. Purification by flash WO 94/14438 PCT/US93/1256~
a~ -chromatography (40% ethyl acetate in petroleum ether as eluent) gave an amide ester interrnediate as white foam (3.7 g).
The foam (2.12 g, 0.003 mol) was dissolved in dry tetrahydrofuran (25 mL) under nitrogen atmosphere, then cooled to 5 -78C. Lithium hexamethyldisilylazide (7 mL, lM solution in tetra-hydrofuran) was added dropwise. After 4 hours, a saturated solution of ammonium chloride was added, and the reaction mixture was allowed to warm to room temperature. The mixture was partitioned between ethyl acetate and water (150 mL each). The ethyl acetate layer was dried o over sodium sulfate,`then concentrated. Purification by flash chromatography (gradient from 15% to 20% ethyl acetate in petroleum ether as eluent) afforded a protected aminosuccinimide intermediate as a white foam.
To a solution of the Boc protected aminosuccinimide (2.7 5 g) in methylene chloride (10 mL) was added trifluoroacetic acid (5 mL). After 3 h, the mixture was concentrated. Purification by flash chromatography (3% methanol in methylene chloride as eluent) afforded the title compound as a white foam (1.6 g).
1 HNMR: consistent with structure.
20 HPLC: method B, Rt = 11.96 min.
FABMS: M + 1 at 500 Analysis calculated for C27H37N3O4S1 + 0.25 methylene chloride C, 62.83; H, 7.26; N, 8.07 Found: C, 62.81; H, 7.19; N, 8.08 2s WO 94/14438 PCT/US93/1256~
' 21S1821 N
oS~ o~ o "N J~OH
H

To a solution of the product of Example 36 (24 mg, 0.05 mmol) in acetonitrile (1 mL) was added glycolic acid (9 mg, 0.06 15 mmol), followed by benzotriazolyl-N-oxy-tris(dimethylamino)-phosonium hexafluorophosphate (26 mg, 0.06 mmol) and diiso-propylethyl~mine (7.8 mg, 0.06 mmol). After 18 h, the mixtl1re was concentrated. The title compound was obtained after purification by preparative HPLC (17 mg).
20 lHNMR: consistent with structure.
HPLC: method B, Rt = 14.46 min.
FABMS: M + 1 at 558 Analysis calculated for C29H39N306S1 + 0.45 trifluoroacetic acid C, 58.96; H, 6.53; N, 6.90 25 Found: C, 59.06; H, 6.72; N, 6.65 21~.18.21 --s ~ ~ .

OSO 0~0 "~N ,I~,OPO(OH)2 o H

To a solution of the product of Example 37 (200 mg, 0.36 mmol) in dry tetrahydrofuran (15 mL) was added diethylamino dibenzylphosphoramidite (171 mg, 0.54 mmol), followed by tetrazole (75 mg, 1.08 mmol). After 18 h at 9C, the solution was cooled to -50C, then m-chloroperbenzoic acid (139 mg) was added and the mixture was allowed to warm to room temperature. After 6 h, the mixture was concentrated, then partitioned between ethyl acetate and 20 aqueous sodium bisulfite. The ethyl acetate was dried over sodium sulfate, then concentrated. Preparative HPLC afforded the intermediate phosphorylated adduct.
The protected phosphate ester obtained above (100 mg) was dissolved in ethanol (10 mL). To this solution was added 10%
25 palladium on carbon (39 mg), then the mixture was placed under a hydrogen atmosphere at 60 psi. After 18 h, the mixture was filtered then concentrated. Preparative HPLC afforded the title compound.
lHNMR: consistent with structure.
HPLC: method B, Rt = 11.98 min.
30 FABMS: M+ 1 at638 WO 94/14438 . PCT/US93/12565 2ls~

Analysis calculated for C29H40N3o9plsl + 1.5 water + 0.6 dioxane C, 52.55; H, 6.71; N, 5.86 Found: C, 52.53; H, 6.42; N, 5.84 ¢~N

~N ~N>
H H

To a solution of the product of Example 29 (24 mg, 0.05 mmol) in methylene chloride (0.5 mL) was added methanol (0.5 mL), 20 followed by histamine dihydrochloride (18 mg, 0.1 mmol) and diisopropylethyl~mine (26 mg, 0.2 mmol). After 18 h a~ room temperature the mixture was concentrated. The title compound was purified by preparative HPLC.
lHNMR: consistent with structure.
25 HPLC: method B, Rt = 11.05 min.
FABMS: M + 1 at 594 Analysis calculated for C32H43N504S 1 + 2.40 trifluoroacetic acid C, 50.96; H, 5.28; N, 8.07 Found: C, 50.92; H, 5.45; N, 8.14 2151821; ~

s ~O,S~O 0~0 N~N~
H

To a solution of the product of Example 29 (48 mg, 0.1 mmol) in methylene chloride (2 mL) was added methanol (2 mL), followed by dimethylaminoethyl~mine (18 mg, 0.2 mmol). After 18 h at room temperature the mixture was concentrated. The title compound was purified by preparative HPLC.
1 HNMR: consistent with structure.
HPLC: method B, Rt = 12.64 min.
20 FABMS: M+ 1 at571 Analysis calculated for C3 1H46N4O4S 1 + 0.55 water C, 64.11; H, 8.18, N, 9.65 Found: C, 64.07; H, 8.08; N, 9.49 N , /~
"S~ 0~0 S~N~

~ 94/14438 PCT/US93/12565 S1 8,2i ' ' To a solution of the product of Example 29 (48 mg, 0. l mmol) in methylene chloride (0.5 mL) was added methanol (0.5 mL), followed by dimethylaminoethyl mercaptan hydrochloride (28 mg, 0.2 mmol) and diisopropylethyl~mine (26 mg, 0.2 mmol). After 18 h at 5 room temperature the mixture was concentrated. The title compound was purified by preparative HPLC.
lHNMR: consistent with structure.
HPLC: method B, Rt = 13.82 min.
FABMS: M + 1 at 588 Analysis calculated for C31H45N304S2 + 1.3 TFA + 0.05 dioxane C, 54.82; H, 6.36; N, 5.68 Found: C, 54.76; H, 6.37; N, 5.84 ~ ~N ~?
OSO O~O

H

To a solution of the product of Example 36 (25 mg, 0.05 mmol) in acetonitrile (5 mL) was added N-alpha-N-im-bis-Boc-L-Histidine (21mg, 0.06 mmol), followed by BOP reagent (26 mg, 0.06 30 mmol) and diisopropylethylamine (7.8 mg, 0.06 mmol). After 18 h at room temperature the mixture was concentrated. Preparative HPLC
afforded the Boc protected intermediate, which was dissolved in TFA (5 mL). After 2.5 h, the mixture was concentrated. The title compound was purified by preparative HPLC.

21~

1HNMR: consistent with structure.
HPLC: method B, Rt = 10.00 min.
FABMS: M + 1 at 637 Analysis calculated for C33H44N6O5S 1 + 2.20 TFA + 1.75 water C, 48.87; H, 5.45; N, 9.14 Found: C, 48.86; H, 5.47; N, 8.96 ~N
,S~ O~O J~H
H'-- --N NH2 To a solution of the product of Example 36 (100 mg, 0.2 mmol) in DMF (10 mL) was added N-alpha-Boc-L-arginine hydro-chloride (75 mg, 0.24 mrnol), followed by hydroxybenzotriazole (35 mg, 0.24 mmol), and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide 25 hydrochloride (50 mg, 0.24 mmol). After 18 h at room temperature the mixture was concentrated. Preparative HPLC afforded the Boc protected inteImediate, which was dissolved in 50% TFA in methylene chloride (6 mL). After 18 h, the mixture was concentrated. The title compound (67 mg) was purifiled by preparative HPLC.
30 lHNMR: consistent with structure.
HPLC: method B, Rt = 9.80 min.
FABMS: M + 1 at 656 Analysis calculated for C33H49N505S1 + 2.5 TFA + 1.45 water C, 47.19; H, 5.67; N, 10.14 Found: C, 47.19; H, 5.62; N, 10.13 a~o 94/14438 PCT I S93/lZSoe ~N ~S~

N~ N NH~
NHAc To a solution of the product of F,x~mple 43 (approx. 0.4 mmol) in methylene chloride (5 mL) was added diisopropylethylamine until the pH was approximately 8.5. Acetyl chloride (31 mg) was added. After 18 h at room temperature the mixture was concentrated.
The title compound (138 mg) was purified by preparative HPLC.
lHNMR: consistent with structure.
HPLC: method B, Rt = 11.99 min.
20 FABMS: M+ 1 at698 Analysis calculated for C35H5 lN706S 1 + 1.7 TFA + 0.1 dioxane C, 51.74; H, 5.99; N, 10.89 Found: C, 51.72; H, 6.13; N, 11.01 2 1 5 1 8 ~ i 1 -((7,7-Dimethyl-2-Oximino-Bicyclo(2.2.1)Heptan- l -yl)-Methane-sulfonyl)-4-(2-Methylphenyl)-3 -Piperazine ¢~CH

--,S~ NOH

To a stirred solution of 1-((7,7-dimethyl-2-oxo-bicyclo-(2.2.1)heptan- 1 -yl)methanesulfonyl)-4-(2-methylphenyl)piperazine (65.0 g; 166 mmol) in pyridine (250 mL) was added hydroxyl~min~
hydrochloride (35.0 g; 0.504 mol). The solution was heated to 70C for 18 h. The solvent was removed under reduced pressure, the residue was taken up in chloroform (500 mL) and washed with aqueous 20 NaHCO3 (2 x 200 mL), water (100 mL), and 5% aqueous HCl (2 x 200 mL). The organic phase was dried (MgSO4), filtered, and the solvent was removed under reduced pressure. The title compound crystallized from ethyl acetate, giving off-white needles (57 g; 84%), mp 174-175C.
25 TLC: Rf 0.40 (75:25 hexane-ethyl acetate) HPLC (method A): retention time 9.98 min FABMS: M + l at 406 Analysis calculated for C21H3 lN303S
C, 62.19; H, 7.71; N, 10.36 30 Found: C, 62.29; H, 7.63; N, 10.15 1H NMR (300 MHz, CDCl3): ~ 7.90 (br s, lH), 7.18 (m, 2H), 7.02 (m, 2H), 3.47 (m, 4H), 4.43 (d, J=14.4 Hz, lH), 3.00 (m, 4H), 2.92 (d, J=14.4 Hz, lH), 2.4-2.6 (m, 2H), 2.31 (s, 3H), 2.09 (d, J=16.9 Hz, lH), 1.95 (m, 2H), 1.80 (m, lH), 1.32 (m, lH), 1.08 (s, 3H), 0.87 (s, 3H) ~0 94/14438 PCT/US93/12~65 _ 7 1 _ 1 -((7,7-Dimethyl-2-Endo-Amino-Bicyclo(2.2. 1 )Heptan- 1 -yl)Methane-sulfonyl)-4-(2-Methylphenyl)-3 -Piperazine ~CH~

,S~ NH2 O O

To a stirred solution of 1-((7,7-dimethyl-2-oximino-5 bicyclo(2.2. 1 )heptan- 1 -yl)methanesulfonyl)-4-(2-methylphenyl)-piperazine (35.0 g; 86 mmol) in 2-methoxyethanol (500 rnL) con~ining Raney Nickel alloy (105.0 g) was added sodium hydroxide solution (17.2 g; 430 mmol dissolved in 75 mL) dropwise over 30 min. During the addition heat and gas was evolved. The mixture was stirred at 20 ambient temperature for 16 h, at which time TLC indicated complete consumption of starting oxime and a ca. 4:1 mixture of endo (lower Rf) and exo (higher Rf) amine products. The mixture was filtered through Celite and the filtercake was washed with methanol and ethyl acetate.
The solvents were removed under reduced pressure and the resulting 25 solid was dispersed in water and filtered. The dried solid was purified by pressurized silica gel column chromatography, using a 93:3 to 94:6 A:B gradient elution (A=chloroform, B=5% NH40H/MeOH). The title compound was obtained as a white foam (24 g; 70%).

WO 94/14438 PCT/US93112~65 2151821 ~

¢~c C H

O O ~ ~0 The procedure of example 2 was carried out using the product of example 46 [1.38 mmol], triethylamine [3.40 mmol], and substituting glycine methyl ester hydrochloride [1.54 mmol] for histidine methyl ester dihydrochloride. The intermediate hydantoin was purified by flash chromatography using 5% methanol in methylene chloride as eluent.
To a stirred solution of the hydantoin (120 mg, 0.253 mmol) in dry tetrahydrofuran (15 mL) was added 2R-(-)-glycidyl 20 tosylate (288 mg, 1.26 mmol), followed by sodium hydride (60%
dispersion in oil). The temperature was increased to reflux. After 2 hours the mixture was cooled then concentrated. Purification by preparative TLC (40% ethyl acetate in petroleum ether as eluent) afforded [1 R-[[4-(2-methylphenyl)piperazin- 1 -yl)sulfonyl] -methyl]7,7-25 dimethylbicyclo[2.2.1]hept-2-endo-yl] -3-[2S ,3 oxirane- 1 -propenyl] - 2,5 - dioxo-l -imidazolidine as a white foam (112 mg).
lHNMR: consistent with structure.
HPLC: method A; Rt = 13.4 min; 98%
Analysis calculated for C27H3gN4O5S + 0.25 methylene chloride C, 59.30; H, 7.03; N, 10.15 Found: C, 59.64; H, 7.10; N, 9.79 ~0 94/14438 PCT/US93/12565 ~l~8~l [3~CH~

OS~ 0~0 AcO OAc To a solution of 1-((7,7-dimethyl-2-endo-amino-bicyclo-(2.2.1)heptan- 1 -yl)methanesulfonyl)-4-(2-methylphenyl)-3-piperazine (103 mg, 0.286 mmol) in methylene chloride (15 mL) was added diacetyl tartaric anhydride (71 mg, 0.315 mmol). After stirring for 1 h at room temperature, the mixture was concentrated, then dissolved in acetic anhydride (20 mL). Sodium acetate (47 mg, 0.572 mmol)was added, then the mixture was heated to 70C. After 40 h, the mixture 20 was cooled to room temperature, then concentrated. Flash chromatography using 20% ethyl acetate in petroleum ether as eluent afforded 61 mg of the title compound as a white foam.
lHNMR: consistent with structure.
HPLC: method A; Rt = 14.16 min; 98%
25 FABMS: M + 1 at 590 Analysis calculated for C29H39N3OgS + 0.15 hexanes + 1.15 ethyl acetate C, 58.71; H, 7.15; N, 6.01 Found: C, 58.71; H, 6.91; N, 5.98 WO 94/14438 PCT/US93/1256~
i ~' '' ~i5182~ --¢~CH~

O,So 0~0 HO OH

To a solution of the product of Example 48 (20 mg, 0.033 mmol) in 3:1 tetrahydrofuran:water (10 mL) at 0C was added hydrogen peroxide (4 eq), followed by lithium hydroxide (2 eq). After stirring at room temperature for 40 min, the mixture was concentrated.
Flash chromatography using 10% methanol in methylene chloride as eluent afforded 14 mg of the title compound as a white foam.
lHNMR: consistent with structure.
20 HPLC: method A; Rt= 11.4 min; 97%
FABMS: M + 1 at 506 Analysis calculated for C25H35N3O6S + 0.25 chloroform + 0.20 water C, 56.26; H, 6.67; N, 7.79 Found: C, 56.27; H, 6.55; N, 7.53 ~CH3 ~/

\=\N
OSO 0~0 The procedure of Example 36 was followed, where the product of example 46 was used in place of endo-[lS]-1'[[[2-amino-7,7-dimethylbicyclo[2.2.1]-hept- 1 yl] -methyl] -sulfonyl] spiro[ l H-indan- 1 -4'-piperidine], and Boc-(L)-Aspartic acid beta-methyl ester was used 5 instead of Boc-(D)-Aspartic acid beta-benzyl ester.
lHNMR: consistent with structure.
HPLC: method A; Rt = 10.82 min; 98%
Analysis calculated for C25H36N4O4S + 0.25 hexanes + 0.50 methylene chloride.
C, 58.68; H, 7.39; N, 10.14 Found: C, 58.73; H, 7.23; N, 10.22 ~N

OSO O~O
N ~,OH

To a solution of the product of Example 50 (50 mg, 0.102 25 mmol) in methylene chloride (15 mL) was added glycolic acid (12 mg, 0.15mmol), followed by 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (29 mg, 0.15 mmol) and 1-hydroxy-benzotriazole (21 mg, 0.15 mmol). After stirring at room temperature for 18 h, the mixture was concentrated. The title compound was 30 purified by preparative HPLC.
lHNMR: consistent with structure.
TLC: Rf = 0.4 (10% methanol in methylene chloride) FABMS: M + 1 at 547 Analysis calculated for C27H37N406S + 0.95 trifluoroacetic acid WO 94/14438 . PCTIUS93/12565 2~5~s~

C, 53.08; H, 5.85; N, 8.57 Found: C, 52.97; H, 6.08; N, 8.17 TABLE

In addition to those compounds specifically exemplified above, additional compounds of the present invention are set forth in tabular form below. These compounds are synthesized by use of the synthetic routes and methods described in the above Schemes and Examples and variations thereof well known to those of ordinary skill in the art, and not requiring undue experimentation. All variables listed in the Tables below are with reference to the following generic structure:

N
SO,~
R

~0 94/14438 PCTtUS93tl2565 ~ ,21 $,~ 8~1 TABLE
R R
N~ \N~O
o~NH o~NH

H ~ ~H3 ~o CH3 O
N~ \ ~0 ~,NH ~ NH2 N4 N~
o~NH o~NH

WO 94/14438 ~
21~1821 TABLE (CONT'D) R R

N~NH o\J~ONH
CH3 ,~
lo HO

O~CHN2H o\~OH

~0 ~N
H

o--~/

o~ H O~ NH4NO

~N
N

~p o~N ~

~0 94114438 PCT/US93/12i6i 2lS1821 TABLE (CONT'D) R R

S

" NHCOCH3 ~ OH
H{ N~
1 ~,NH

O~ ~ N

NH --CO2CH3 HN~`~

O N~O
o N~O ~4 ~4 HN--\_ N~
H C2H ~NH

~ os~ H~OH

O ' O

~"'N~NJ~NH ~g"'N~f OH

TABLE (CONT'D) R R

~g"NJ~ ~ "~ N

, o 6~S CO2Et O~N~OH

2 0~ ~o_ p_ O H o~N ~ N H2 O O

'~ O~NH CO2H

O O

30 o~N~CO CH H H CH3 ~ 94/14438 PCT/US93/12565 æ~ s~ $21 TABLE (CONT'D) R R

H~OH o ~Nl ~

0 ~ OH ~NH~
O

'N--4 ;0- P- o H ~ HN--CO2H

o o~ N ,CO2C H3 'N O H /--\o O

~ N NJ 5N OH /--\SO~

~151~Zl TABLE (CONT'D) R R

N N~ ~ ~N~/ N~/--OH

~S~

O

~NA~ H ~ N~,CN
~~N~ N H2 0 o IOI~OH

~N~ SCH3 o~ / ~
2 5 N N - O--P' O H O~ H pl,O H

~ 94/14438 PCT/US93/12565 ~ ~I Sl ~21 TABLE (CONT'D) R R

N N CN "~ H CO2CH3 RADIOLMAND BINDING ASSAYS

The high affinity binding of [3H] Oxytocin (OT)([tyrosyl, 3,5-[3H]oT; 30-60 Ci/mmol; New Fn~l~nd Nuclear. Boston, MA) to uterine OT receptors was based on an assay (Fuchs, A-R; Fuchs, F;
20 Soloff, MS. 1985 J. Clin. Endocrinol. Metab. 60:37) using a crude membrane preparation of uteri taken from diethylstilbestrol dipropionate (DES)-treated (0.3 mg/kg, ip; 18-24) rats. Competition studies were conducted at equilibrium (60 minutes; 22C) using 1 nM[3H]OT in the following assay buffer: 50 mM Tris-HCl,5 mM
25 MgCl2, and 0.1% BSA, pH 7.4. Nonspecific binding (10% of ~e total binding) was determined using 1 ,uM unlabeled OT and the binding reaction was termin~ted by filtration through glass fiber f1lters using a cell harvester (model 7019, Skatron, Inc., Sterling, VA). IC50 (the concentration of tested compound that inhibits 50% of OT) was 30 reported, unless otherwise noted.
The measurement of [3H]Vasopressin (AVP) ([phenylalanyl-3,4,5-3H]AVP; 80-90 Ci/mmol; New F.n~l~nd Nuclear) binding to a crude membrane preparation of male rat liver (AVP-Vl sites) or kidney medulla (AVP-V2 sites) was determined according to the method of Butlen, et al. (Butlen, D; Guillon, G;
Rajerison, R.M.; Jard, S; Sawyer, W.H.; M~nning, M. 1978 Mol Ph~rm~col 14:1006).
Competition assays were conducted at equilibrium (30 minlltes at 30C) using 1 nM [3H]AVP (liver) or 2 nM [3H]AVP
(kidney) in the following assay buffer: 100 mM Tris-HCl, 5 mM
MgC12, 0.1% BSA, 50 mM phenylmethylsulfonylfluoride, and 50 o mg/ml bacitracin, pH 8Ø Nonspecific binding (5-10% of ~e total binding) was determined using 10 ,uM unlabeled AVP, and the binding reaction was termin~ted by filtration as described above for the [3H]oT
binding assay.
IC50 values were determined for both [3H]oT and [3H]AVP binding assays by linear regression of the relation log concentration of compound vs. percent inhibition of specific binding.

Example Result For r_HlOT
29 70% inhib. @ 1000 nM
32 29nM
38 4.9 nM
44 1.0 nM
48 67 nM
49 68nM

While the invention has been described and illustrated with reference to certain preferred embodiments thereof, those skilled in the art will appreciate that various changes, modifications and substitutions 3 can be made therein without departing from the spirit and scope of the invention. For example, effective dosages other than the preferred dosages as set forth hereinabove may be applicable as a consequence of variations in the responsiveness of the m~mm~l being treated for prevention of preterm labor, or for other indications for the compounds ~0 94/14438 PCT/US93/1256i 2 ~ 2 i ~

of the invention indicated above. Likewise, the specific pharmacological responses observed may vary according to and depending upon the particular active compound selected or whether there are present pharmaceutical carriers, as well as the type of 5 formulation and mode of ~lmini~tration employed, and such expected variations or differences in the results are contemplated in accordance with the objects and practices of the present invention. It is intended, therefore, that the invention be limited only by the scope of the claims which follow and that such claims be interpreted as broadly as is reasonable.

Claims (20)

WHAT IS CLAIMED IS:
1. A compound of the formula or a pharmaceutically acceptable salt thereof, wherein X is or a is a single or double bond, R is Het, wherein Het is a substituted saturated or unsaturated heterocyclic ring wherein said substituents are independently one or more of R1, R2, R3, Alk-R1, Alk-R2, Alk-R3, -NHC(O)-Alk-R2R3, -NR5-Alk-R2R3 or Alk-R2R3;
where Alk is C1-10 alkyl and R1, R2 and R3 are independently selected from the group consisting of hydrogen, halogen, C2-10 alkenyl, methylene, C1-10 alkoxycarbonyl, C1-10 alkoxycarbonyl-C1-10 alkylamino, C1-10 alkoxycarbonylamino, C1-10 alkylamino-C1-10 alkylaminocarbonyl, C1-10 alkylcarbonylamino, -S-R4, C1-10 alkylcarbonyloxy, C1-10 alkylsulfonyl, C1-10 alkylthio, amino, amino C1-10 alkylcarbonylamino, amino C1-10 alkylamino, carbonylamino, carbamoyl, carboxyl C1-10 alkylamino, carboxyl, cyano, di-C1-10 alkylamino, di-C1-10 alkylamino-C1-10 alkylamino, di-C1-10 alkylamino-C1-10 alkylthio, di-C1-10 alkylamino-C1-10 alkylaminocarbonyl, guanidinyl, hydroxyl, hydroxyl C1-10 alkylamino, imidazolyl, imidazolyl amino, imidazolyl C1-10 alkylamino, imidazolyl C1-10 alkylaminocarbonyl, morpholinyl, thiomorpholinyl, dioxothiomorpholinyl, indolyl, oxo, oxiranyl, phenyl, piperidinylamino, piperazinyl, pyrrolidinyl, sulfonyl, tetrazolyl C1-10 alkyl-carbonylamino, tetrazolylaminocarbonyl, phosphoryl, phosphoryl C1-10 alkylamino and thiono;
R4 is selected from the group consisting of imidazolyl, C1-10 alkoxycarbonyl-C1-10 alkyl, di-C1-10 alkylamino-C1-10 alkyl and C1-5 alkyl; and R5 is selected from the group consisting of hydrogen and C1-5 alkyl.
2. The compound as claimed in Claim 1, wherein Het is a mono, di, tri or tetra substituted saturated or unsaturated 5 or 6 membered heterocyclic or 7 to 10 membered heterobicyclic ring containing 1, 2 or 3 nitrogen atoms, and R1, R2 and R3 are independently selected from the group consisting of hydrogen, halogen, C2-10 alkenyl, methylene, C1-10 alkoxycarbonyl, C1-10 alkoxycarbonyl-C1-10 alkylamino, C1-10 alkylcarbonylamino, C1-10 alkylcarbonyloxy, C1-10 alkylsulfonyl, -S-R4, amino, amino-C1-10 alkylcarbonylamino, amino C1-10 alkylamino, carbamoyl, carboxyl C1-10 alkylamino, carboxyl, cyano, di-C1-10 alkylamino, di-C1-10 alkylamino-C1-10 alkylamino, di-C1-10 alkylamino-C1-10 alkylthio, di-C1-10 alkylamino-C1-10 alkylaminocarbonyl, guanidinyl, hydroxyl, hydroxyl C1-10 alkylamino, imidazolyl, imidazolyl amino, imidazolyl C1-10 alkylamino, morpholinyl, thiomorpholinyl, dioxothiomorpholinyl, indolyl, oxo, oxiranyl, phenyl, piperidinylamino, piperazinyl, sulfonyl, phosphoryl, phosphoryl C1-10 alkylamino and thiono.
3. The compound as claimed in Claim 2, wherein said bicyclic ring is bonded to one of said heterocyclic or heterobicyclic ring's nitrogen atoms.
4. The compound as claimed in Claim 3, wherein X is and R1, R2 and R3 are independently selected from the group consisting of hydrogen, halogen, C2-10 alkenyl, C1-10 alkoxycarbonyl, C1-10 alkoxycarbonyl-C1-10 alkylamino, C1-10 alkylcarbonylamino, C1-10 alkylsulfonyl, -S-R4, amino, amino-C1-10 alkylcarbonylamino, amino C1-10 alkylamino, carbamoyl, carboxyl, cyano, di-C1-10 alkylamino, di-C1-10 alkylamino-C1-10 alkylamino, di-C1-10 alkylamino-C1-10 alkylthio, guanidinyl, hydroxyl, hydroxyl C1-10 alkylamino, imidazolyl, imidazolyl amino, imidazolyl C1-10 alkylamino, morpholinyl, thiomorpholinyl, dioxothiomorpholinyl, indolyl, oxo, phenyl, piperidinylamino, piperazinyl, sulfonyl, phosphoryl, phosphoryl C1-10 alkylamino and thiono.
5. The compound as claimed in Claim 3, wherein Het is selected from the group consisting of imidazolyl, imidazolinyl, imidazolidinyl, pyrrolyl, dihydropyrrolyl, pyrrolidinyl, piperazinyl, triazaspirodecane, pyrrolo-isoxazole, pyrrolo-pyrazole and pyrrolo-pyrrole.
6. The compound as claimed in Claim 1, wherein X is R is Het, wherein Het is a mono, di, tri or tetra substituted saturated or unsaturated 5 or 6 membered heterocyclic ring containing 1, or 2 nitrogen atoms that is bonded to said bicyclic ring at one of said heterocyclic ring's nitrogen atoms, wherein said substituents are independently one or more of R1, R2, R3, Alk-R1, Alk-R2 ,Alk-R3 or Alk-R2R3; and where Alk is C1-10 alkyl and R1, R2 and R3 are independently selected from the group consisting of hydrogen, C2-10 alkenyl, C1-10 alkoxycarbonyl, C1-10 alkoxycarbonyl-C1-10 alkylamino, C1-10 alkoxycarbonylamino, C1-10 alkylamino-C1-10 alkylaminocarbonyl, C1-10 alkylcarbonylamino, C1-10 alkylcarbonyloxy, C1-10 alkylsulfonyl, C1-10 alkylthio, amino, amino-C1-10 alkylcarbonylamino, carbonylamino, carboxyl C1-10 alkylamino, carboxyl, cyano, di-C1-10 alkylamino, di-C1-10 alkylamino C1-10 alkylaminocarbonyl, guanidinyl, hydroxyl, imidazolyl, imidazolyl C1-10 alkylaminocarbonyl, indolyl, oxo, phenyl, piperidinylamino, piperizinyl, pyrrolidinyl, sulfonyl, tetrazolyl C1-10 alkylcarbonylamino, tetrazolylaminocarbonyl and thiono.
7. The compound as claimed in Claim 6, wherein a is a single bond, and R1, R2 and R3 are independently selected from the group consisting of hydrogen, C2-10 alkenyl, C1-10 alkoxycarbonyl, C1-10 alkoxycarbonyl-C1-10 alkylamino, C1-10 alkylcarbonylamino, C1-10 alkylcarbonyloxy, C1-10 alkylsulfonyl, amino, amino-C1-10 alkylcarbonylamino, carbonylamino, carboxyl, cyano, di-C1-10 alkylamino, di-C1-10 alkylamino-C1-10 alkylaminocarbonyl, guanidinyl, hydroxyl, imidazolyl, imidazolyl C1-10 alkylaminocarbonyl, indolyl, oxo, phenyl, piperidinylamino, piperazinyl, sulfonyl and thiono.
8. The compound as claimed in Claim 7, wherein Het is selected from the group consisting of imidazolyl, imidazolinyl, imidazolidinyl, pyrrolyl, dihydropyrrolyl, pyrrolidinyl and piperazinyl.
9. The compound as claimed in Claim 2, wherein X is and R1, R2 and R3 are independently selected from the group consisting of hydrogen, halogen, C2-10 alkenyl, C1-10 alkoxycarbonyl, C1-10 alkoxycarbonyl-C1-10 alkylamino, C1-10 alkylcarbonylamino, C1-10 alkylcarbonyloxy, C1-10 alkylsulfonyl, -S-R4, amino, amino-C1-10 alkylcarbonylamino, amino C1-10 alkylamino, carbamoyl, carboxyl, cyano, di-C1-10 alkylamino, di-C1-10 alkylamino-C1-10 alkylamino, di-C1-10 alkylamino-C1-10 alkylthio, guanidinyl, hydroxyl, hydroxyl C1-10 alkylamino, imidazolyl, imidazolyl amino, imidazolyl C1-10 alkylamino, morpholinyl, thiomorpholinyl, dioxothiomorpholinyl, indolyl, oxo, oxiranyl, phenyl, piperidinylamino, piperazinyl, sulfonyl, phosphoryl, phosphoryl C1-10 alkylamino and thiono.
10. The compound as claimed in Claim 9, wherein R1, R2 and R3 are independently selected from the group consisting of hydrogen, C1-10 alkylcarbonyloxy, amino, hydroxyl, oxo, phosphoryl and oxiranyl.
11. The compound as claimed in Claim 5, selected from the group consisting of and
12. A pharmaceutical composition comprising a pharmaceutically acceptable carrier and a pharmacologically effective amount of the compound as claimed in Claim 1, sufficient to antagonize oxytocin from binding to its receptor site.
13. A pharmaceutical composition comprising a pharmaceutically acceptable carrier and a pharmacologically effective amount of the compound as claimed in Claim 1 sufficient to prevent preterm labor in a mammal in need thereof.
14. A pharmaceutical composition comprising a pharmaceutically acceptable carrier and a pharmacologically effective amount of the compound as claimed in Claim 1, sufficient to stop labor preparatory to cesarian delivery.
15. A pharmaceutical composition comprising a pharmaceutically acceptable carrier and a pharmacologically effective amount of the compound as claimed in Claim 1, sufficient to treat dysmenorrhea.
16. A method of antagonizing oxytocin from binding to its receptor site in a mammal, comprising the step of administering to said mammal a pharmacologically effective amount of the compound as claimed in Claim 1.
17. A method of preventing preterm labor in a mammal in need thereof, comprising the step of administering to said mammal a pharmacologically effective amount of the compound as claimed in Claim 1.
18. A method of stopping labor preparatory to cesarian delivery in a mammal in need thereof, comprising the step of administering to said mammal a pharmacologically effective amount of the compound as claimed in Claim 1.
19. A method of treating dysmenorrhea in a mammal in need thereof, comprising the step of administering to said mammal a pharmacologically effective amount of the compound as claimed in Claim 1.
20. A compound of the formula or a pharmaceutically acceptable salt thereof, wherein X is or a and b represent a single or double bond, R is selected from the group consisting of , and R2 is selected from the group consisting of -AlkR5R6, -NH-C(O)-Alk-R7R8, -N(R4)-Alk-R7R8, amino C1-10 alkylcarbonylamino, piperidinylamino, oxiranyl C1-10 alkyl, imidazolylamino, C1-10 alkoxycarbonyloxy, hydroxyl, phosphoryl, -S-R9, C1-10 alkylcarbonyloxy and C1-10 alkylcarbonylamino;
Alk is C1-10 alkyl;

R3 is selected from the group consisting of hydrogen, hydroxyl, phosphoryl, C1-10 alkylcarbonyloxy and C1-10 alkoxycarbonyl;
R4 is selected from the group consisting of hydrogen, benzyl and C1-10 alkyl;
R5 and R6 are independently selected from the group consisting of hydroxyl, di-C1-10 alkylamino, piperazinyl, halogen, phosphoryl, morpholinyl, thiomorpholinyl, dioxothiomorpholinyl, hydroxyl C1-10 alkylamino, cyano and -SCH3;
R7 and R8 are independently selected from the group consisting of hydrogen, hydroxyl, hydroxyl C1-10 alkyl, C1-10 alkylcarbonylamino, amino, phosphoryl, imidazolyl, di-C1-10 alkylamino, guanidinyl, C1-10 alkoxycarbonyl, carboxyl and C1-10 alkoxycarbonylamino;
R9 is selected from the group consisting of di-C1-10 alkylamino-C1-10 alkyl, imidazolyl and C1-10 alkoxycarbonyl-C1-10 alkyl;
provided that R5 and R6 cannot both be hydroxyl.
CA002151821A 1992-12-23 1993-12-23 Hydantoin and succinimide-substituted derivatives of spiroindanylcamphorsulfonyl oxytocin antagonists Abandoned CA2151821A1 (en)

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US5464788A (en) * 1994-03-24 1995-11-07 Merck & Co., Inc. Tocolytic oxytocin receptor antagonists
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US7781478B2 (en) 2004-07-14 2010-08-24 Ptc Therapeutics, Inc. Methods for treating hepatitis C
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