CA2148006C - Use of non-steroidal antiestrogens for autoimmune diseases - Google Patents

Abstract

Disclosed is use of nonsteroidal anti-estrogen compounds such as toremifene citrate as active ingredient for treating autoimmune diseases.

Description

~~~ 19/10 DESCRIPTION
1 (Technical Field]
The present invention relates to use of nonsteroidal anti-estrogen compounds (hereinafter referred to as nonsteroidal anti-estrogens) such as toremifene, expected as a remedy for autoimmune diseases.
The autoimmune diseases include collagen diseases and the like. In light of affected parts by the diseases, there are mentioned, for example, degenerative diseases of supporting tissues and connec-tive tissues; autoimmune degenerative diseases of salivary glands, particularly Sjbgren~s disease;
autoimmune degenerative diseases of kidneys, particular-ly systemic lupus erythematodes and glomerulonephritis;
autoimmune degenerative diseases of joints, particularly rheumatoid arthritis; and sutoimmune degenerative diseases of blood vessels such as generalized necrotizing angitis and granulomatous angitis; and multiple sclerosis.
[Background Art]
Immunosuppressants, nucleic acid antagonists, antimetabolites, etc., are used in the medicinal treat-ment of autoimmune diseases today. Anti-inflammatory agents, anticoagulants, etc., are also used in the 1 symptomatic therapies of the diseases. The effects of these agents are, however, not yet sufficient.
It is known that the immunosuppressants have side effects of provoking diabetes, renal disorders, infectious diseases, etc. Also the use of the nucleic acid antagonist or antimetabolite is frequently accompanied by side effects such as hepatic disorders and medullary disorders. Thus the medicinal treatment of autoimmune diseases is so far very insufficient.
It has been demanded to develop a remedy for autoimmune diseases which acts on the immune system and which has a function mechanism different fxom that of conventional drugs for the diseases and less serious side effects.
[Disclosure of Invention]
After intensive investigations made for the purpose of finding the above-described remedy, the present inventors have found that nonsteroidal anti-estrogens have an excellent therapeutic effect on the autoimmune diseases and thus, based on this finding, completed the present invention.
The present invention relates to a remedy for autoimmune diseases which comprises as active ingredient a nonsteroidal anti-estrogen or a pharmaceutically acceptable salt thereof.

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1 [Brief Description of Drawings]
Fig. 1 shows survival times of animals (NZBxNZW F1 mice: B/W F1 mice) which accepted different doses of toremifene.
[Best Mode for Carrying Out the Invention]
The nonsteroidal anti-estrogen compounds usable in the present invention are those having a triphenyl CZ - C5 alkene or triphenyl C2 - C5 alkane skeleton. Preferably, they are CZ - C5 alkenes or CZ - C5 alkanes having three phenyl substituents at the 1- .
position and 2-position, wherein any of the phenyl groups may have a substituent such as a mono- or di-lower alkyl ( C1 - C3 ) amino lower alkoxy ( G~ - C3 ) group, or a hydroxyl group, or the alkyl group in the above alkenes or alkanes may have a substituent such as a halogen.
Examples of these compounds include toremifene (JP-B-4 19973), tamoxifen (JP-B-59 21861), 4-hydroxy- , tamoxifen (JP-A-54 44644), 3-hydroxytamoxifen (JP-A-57 122049) and N-demethyltoremifene or 4-hydroxytoremifene (JP-A-3 163015). Toremifene is particularly preferred.
,Ia is well-known that these compounds have an,anti~
neoplastic effect (see Cancer Chemotherapy and Pharmacology, 17, 109-113 (1986) and the above-mentioned patent publications).
The pharmaceutically acceptable salts thereof include, for example, hydrochlorides, sulfates, 1 citrates, tartrates and phosphates.
Drugs usable in combination with the nonsteroidal anti-estrogens in the medicinal treatment of autoimmune diseases include glucocorticoids (e. g.
prednisoione, prednisone, cortisol). Prednisolone is preferred.
The glucocorticoids themselves have an effect of treating the autoimmune diseases. The nonsteroidal anti-estrogens or a pharmaceutically acceptable salt thereof according to the present invention concomitant with the glucocorticoids synergistically improve the effect of treating.
The remedy of the present invention particularly exhibits an excellent remedial effect on systemic lupus erythematodes.
Therefore the present invention relates to the following:
(i) a remedy for autoimmune diseases which comprises as active ingredient a nonsteroidal anti-estrogen or a pharmaceutically acceptable salt thereof;
(ii) a remedy recited in (i), wherein the nonsteroidal anti-estrogen is a compound having a triphenyl Cz-C~ alkene or triphenyl C2-C5 alkane skeleton;
(iii) a remedy recited in (i) or (ii), wherein the active ingredient is toremifene or a pharmaceutically acceptable salt thereof;
(iv) a remedy recited in (i) or (ii), wherein the autoimmune diseases are collagen diseases, autoimmune 21~~(~0 1 degenerative diseases of kidneys such as nephritis, particularly glomerulonephritis, and autoimmune degenerative diseases of blood vessels, salivary glands and joints;

5 (v) a remedy recited in (i) or (ii), wherein the autoimmune diseases are systemic lupus erythematodes;

and (vi) a remedy recited in (i) or (ii) for concomitant use with a glucocorticoid.

The pharmaceutical composition of the present invention is administered orally, parenterally or intravenously.

Usually, a pharmaceutically effective amount of the active ingredient is used in combination with a suitable medicinal carrier or other auxiliaries. The term "pharmaceutically effective amount" herein means an j amount capable of exhibiting the intended pharmacolog-i ical activity without causing unfavorable side effects.

The accurate amount varies in each case depending on various factors such as administration methods, individual natures of the patients and situations in which the.patient accepts the remedy and, as a matter of course, structures of derivatives to be administered.

Dose of the active ingredient for adult is usually 10 to 1000 mg/day, preferably 20 to 500 mg/day, more preferably 30 to 300 mg/day.

In the case of the concomitant use, dose of the glucocorticoid for adult is 1 to 100 mg/day, 2~.48~~
_ 6 _ 1 preferably 2 to 60 mg, and that of the nonsteroidal anti-estrogen or the pharmaceutically acceptable salt thereof for adult is 10 to 700 mg/day, preferably 20 to 500 mg/day, more preferably 30 to 300 mg/day.
The medicinal carrier or other auxiliaries generally usable in combination with the active ingredient according to the present invention may be any of solid and liquid ones and usually selected in consideration of an administration route. Examples of the solid carrier include lactose, sucrose, gelatin and agar, and those of the liquid carrier include water, syrup, peanut oil and olive oil. Other suitable carriers and auxiliaries known by those skilled in the art are also usable. The active ingredient according to the present invention can be combined with the carrier or other auxiliaries to form any of various acceptable preparations such as tablets, capsules, suppositories, liquid, emulsion and powder.
In the preparations of the remedy of the present invention, the amount of the nonsteroidal anti-estrogen or the pharmaceutically acceptable salt thereof can widely vary depending on the preparation, etc.
Usually, the amount is 0.01 .., 100 by weight, preferably 0.1 ,..70~ by weight, and the balance contains the medicinal carrier or other auxiliaries.
MRL/Mp-lpr/lpr mice spontaneously develop a lethal glomerulonephritis, angitis, sialadenitis, polyarthritis, etc., concurrently with the deposition of 2~~~~~

1 an immune complex with age. Therefore, they are widely used as experimental models for human systemic lupus erythematodes, Sjogren's disease, rheumatoid arthritis and autoimmune angitis such as multiple arteritis.
The present invention will be explained referring to examples on suppression of lymphadenopathy glomerulonephritis, angitis, sialadenitis and arthritis of MRL/Mp-lpr/lpr mice with the nonsteroidal anti-estrogen compound according to the present invention.
The nonsteroidal anti-estrogen such as toremifene and the pharmaceutically acceptable salt thereof according to the present invention exhibit an excellent remedial effect on degenerative diseases such as autoimmune diseases, for example, systemic lupus erythematodes.
Example 1 Treatment of spontaneous autoimmune diseases of MRL/Mp-lpr/lpr mice by administration of 2f4-(Z)-4-chloro-1,2-diphenvl-1-butenvl,]phenol-N,N-dimethvlethvlamine citrate (toremifene citrate) Eight-week old female MRL/Mp-lpr/lpr mice (Clea Japan, Inc.) were used in this examination.
Toremifene citrate (JP-B-4 19973) was suspended in carboxymethylcellulose to prepare a 0.5~ suspension.
This compound (100 mg/kg) was orally administered to each mouse once a day for 13 weeks. ..

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_8_ 1 (A) Tnhibition of swelling of spleen and lymph node of MRL/Mp-lpr/lpr mice with toremifene citrate Repeated oral administration of 100 mg/kg of toremifene citrate once a day for 13 weeks inhibited the swelling of the spleen and lymph node of each mouse (see Table 1).
The spleen and lymph nodes of the MRL/Mp-lpr/lpr mice are seriously swollen with age due to the presence of the lymphoproliferation gene (lpr). The lpr codes for the Fas antigen in each mouse. However, in the MRL/Mp-lpr/lpr mice, an abnormality of the genes disturbs the expression of the Fas antigen. As a result, autoreactive T-cells are not subjected to negative selection through the Fas antigen in the thymus and appear in the peripheral tissues to cause the swelling of the lymphoid organs and autoimmune symptoms.
The presence of the autoreactive T-cells was confirmed also in the autoimmune diseases of human beings, such as rheumatoid arthritis.
The results of this study indicated that the nonsteroidal anti-estrogen compounds such as toremifene citrate are capable of inhibiting the appearance of the ,autoreactive T-cells, thereby suppressing the swel~.ing , of spleen and lymph node to treat the autoimmune diseases.

y Table 1: Effect of toremifene citratel~ on swelling of spleen and lymph node MRL/Mp-lpr/lpr mice Number Spleen weight Lymph node of 4~ 5~

Group animals Body weight weight Body weight Control 11 2.340.74 3~ 6.771.70 2~

Toremifene citrate 12 1.381.06 3.111.43 treatment 1 1) Toremifene citrate (100 mg/kg) was orally administered to 8-week old mice once a day for 13 weeks.
2) Only 0.5~ carboxymethylcellulose was given to the mice of the control group.
3) Standard deviation Weight of spleen X 100 4) Spleen weight/body weight = Body weight of mouse 5) Lymph node weight/body = Weight of lymph node x 100 weight Body weight of mouse (B) Suppression of renal disorder of MRL/Mp-lpr/lpr mouse with toremifene citrate An autopsy was performed on the mice of the control group and the toremifene citrate treated group after the completion of the administration to examine their kidneys pathohistologically. The blood urea nitrogen (BUN) of the serum in each group was examined to confirm changes in the renal function. As shown in 21~~~~
- to -1 Table 2, toremifene citrate ameliorated the glomerulonephritis and healed the renal function in the MRL/Mp-lpr/lpr mice.
The glomerulonephritis of the MRL/Mp-Ipr/lpr mice is caused by the deposition of immunocomplexes.
Also in the case of the autoimmune diseases such as systemic lupus erythematodes (SLE) of human, the patients suffer from glomerulonephritis concurrent with the deposition of the immunocomplex. The results indicated that the nonsteroidal anti-estrogen compounds such as toremifene citrate are effective remedies for the degenerative diseases of the kidney, such as the SLE
with renal syndrome and glomerulonephritis.
Table 2: Improvement of renal function and amelioration of glomerulonephritis of MRL/Mp-lpr/lpr mice with toremifene citrate Number Glomerulonephritis BUN
1~

Group of (mg/dl )2~

animals Control 11 2.4 0.7 3~ 43.123.9 Toremifene citrate 12 1.2 0.7 24.614.9 treatment 1) The kidney was fixed in 10~ buffered formalin, and then paraffin sections thereof were prepared by an ordinary method to prepare HE and PAS stained specimens. The extent of the disorder of the renal ~1~~QQ~
_ 11 _ 1 glomeruli was scored and classified into the following groupsn 0 (no disorder), 1 (slight disorder), 2 (medium disorder), and 3 (heavy disorder).
Twenty-five renal glomeruli were observed for each mouse and the average thereof was calculated.
2) The BUi~ was determined with a Fuji Dry Chem Analyzer.
3) Standard deviation.
(C) Inhibition by toremifene citrate of sialadenitis an~c~itis and arthritis of MRL/Mp-lpr/lpr :.:.
mice The salivary gland, renal blood vessel and knee joint of each mouse in the control group and the toremifene citrate treated group were histopathological-ly examined.
As shown in Table 3, toremifene citrate prevented the mice from being attacked by sialadenitis, angitis and arthritis.
These results indicated that the nonsteroidal ,anti-estrogen compounds such as toremifene citrate and tamoxifen citrate can be used as the remedy for autoimmune sialadenitis (SjSgren's disease), autoimmune arthritis (chronic articular rheumatism) and autoimmune angitis (necrotizing angitis and granulomatous angitis).

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Table 3: Effect of toremifene citrate for preventing MR~/Mp-lpr/lpr mice from being attacked by sialadenitis, angitis and arthritis Number Sialadenitis Angitis Arthritis Group of 1) 1) 1) animals Control 11 2.20.6 Z~ 2.10.7 1.60.9 Toremifene citrate 12 0.90.8 0.90.8 0.40.5 treatment 1 1) The salivary gland, kidney and knee joint were fixed in 10~ buffered formalin, and then paraffin sections thereof were prepared by an ordinary method to prepare HE and PAS stained specimens. The extent of the disorder was scored and classified into the following groups:
0 (no disorder), 1 (slight disorder), 2 (medium disorder), and 3 (heavy disorder).
2) Standard deviation.

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1 Example 2 Effect of concomitant use of toremifene citrate with alucocorticoid on MRL/Mp-lpr/lpr mice Twelve-week old female MRL/Mp-lpr/lpr mice were used in the examination. Thirty miligrams per kg or 15 mg/kg of toremifene citrate (TOR) was orally -,:
administered to each mouse twice a day for 9 weeks from the 12th week to the 21st week. A glucocorticoid (prednisolone), 8, 4 and 2 mg/kg/day, were subcutaneous-ly administered to mice once a day as a positive control drug. The concomitant use of tremifene with the glucocorticoid was also carried out according to the same regimen as above. The kidney was taken out from each mouse the day after the completion of the whole administration period and fixed in a PLP fixative.
Frozen sections were made from the fixed kidney and used for an immunostaining with an anti-Mac-2 monoclonal antibody (Hybritec Inc., San Diego, USA). The number of Mac-2 positive cells (activated macrophages) invading each of 10 to 20 glomeruli of the kidney, which is hereinafter referred to as Mac 2 number, was counted under a microscopy to determine an average Mac 2 number per glomerulus. The degree of severeness of glomerulonephritis was estimated in terms of the average Mac 2 number (n = 13 for each group). Table 4 shows the results.

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Table 4: Suppression of glomerulonephritis of MRL/Mp-lpr/lpr mice by concomitant use of toremifene citxate with glucocorticoid Group Mac 2 number Control 7.5 ~ 1.5 Toremifene citrate (TOR) 30 mg/kg 6.2 ~ 1.0 15 mg/kg 6.5 ~ 1.2 Prednisolone (P) 8 mg/kg 5.8 ~ 0.8 4 mg/kg 7.9 ~ 0.7 2 mg/kg 9.4 ~ 1.0 Control 11.3 ~ 1.2 Prednisolone (P) 4 mg/kg 9.1 ~ 1.4 2 mg/kg 7.7 ~ 1.0 P 4 mg/kg & TOR 30 mg/kg (concomitant use) 4.1 ~ 0.5*
P 4 mg/kg & TOR 15 mg/kg (concomitant use) 4.3 t 0.5*
P 4 mg/kg & TOR 7.5 mg/kg (concomitant use) 3.5 * 0.5*
P 2 mg/kg & TOR 30 mg/kg (concomitant use) 3.6 ~ 0.7*
P 2 mg/kg & TOR 15 mg/kg (concomitant use) 2.8 ~ 0.5*
P 2 mg/kg & TOR 7.5 mg/kg (concomitant use) 4.3 ~ 0.6*
* P < 0.01 (t-test) 1 All the groups treated by concomitant use of toremifene citrate (TOR) with prednisolone (P) exhibited significant decrease in Mac 2 number as compared with the control and the prednisolone treated group. On the 2~.~8~~J~v , 1 other hand, the prednisolone treated group and the toremifene citrate treated group did not exhibit any significant decrease in Mac 2 number as compared with the control. The results of these tests indicates that the concomitant use of the both drugs synergistically suppresses the glomerulonephritis.
Example 3 Comparison of survival time NZB x NZW mice (B/W F1 mice) were used as a pathological model of autoimmune diseases (systemic lupus erythematodes). Effect of toremifene citrate on the survival time of the animals was investigated.
Experimental animals:
F1-hybrids of NZB (female) and NZW (male) mice (B/W F1 mice): Imported from,BOmholtgaard, Denmark at the age.of five weeks.
Test aroups and doses:
Control (male): administration polyethyleneglycol (peg) 3 times a week per os Control (female): administration peg 3 times a week per os Toremifene citrate 30 mg/kg/day: administration 70 mg/kg in polyethylene glycol solution 3 times a week per os to female NZB x NZW F1 mice Toremifene citrate 3 mg/kg/day: administration 7 mg/kg in polyethylene glycol solution 3 times 2~.4~f~~

1 a week per os to female NZB x NZW F1 mice The survival time of the animals in different test groups is presented in Fig. 1. All but two female control animals have died during the almost two years' follow-up time. Fifty percents of the animals in this group died before/at the age of 40 weeks, and 20~ (4/20) were alive after one year.
In the male control group, five animals died during the first 24 Weeks (not shown in Fig. 1) due to aggressive behaviour and thereby acquired infection.
These five were excluded from the results. Forty-seven percents of the male control mice are still alive after almost two years' time.
In both toremifene treatment groups the life span of the animals has lengthened clearly when compared to the female control animals. In the 3 mg/kg toremifene treatment group only one (1120) animal had died at/before the age of 40 weeks and three (3/20) animals in the 30 mg/kg toremifene group.
After one year 80~ and 85$ of the animals were alive in the 3 mg/kg and 30 mg/kg toremifene treated ,groups, respectively, which is nearer the percentage of, the male control animals (~ 90~) than that of the female control group (20~).
Moreover, 25~ (5/20) and 10~ (2120) of the animals are still alive after almost two years' time in the lower and higher toremifene dosage group, respec-- 1~ ..
1 tively.
The follow-up data of 60 female and 15 male F1-hybrids of NZB x NZW F1 mice (B/W F1 mice) show that toremifene treatment has clearly extended the life span of female mice.
Example 4 Examples of preparations comprising the nonsteroidal anti-estrogen or the pharmacologically acceptable salt thereof as active ingredient will be given below, which by no means limit the preparations of the present invention.
Preparation Example 1 Formulation of prepared 200 mg tablet.
Toremifene citrate 20 mg Starch 85 mg Lactose 90 mg Magnesium stearate 5 mg Preparation Example 2 Formulation of prepared 200 mg tablet.
Tamoxifen citrate 20 mg Starch 85 mg Lactose 90 mg Magnesium stearate 5 mg

Claims (16)

The embodiments of the invention in which an exclusive property or privilege is claimed are defined as follows:

1. A pharmaceutical composition comprising toremifene, or a pharmaceutically acceptable salt thereof, together with a pharmaceutically acceptable carrier, for the treatment of an autoimmune disease.

2. A pharmaceutical composition according to claim 1, wherein said autoimmune disease is an autoimmune degenerative disease of kidneys.

3. A pharmaceutical composition according to claim 1, wherein said autoimmune disease is an autoimmune degenerative disease of salivary glands.

4. A pharmaceutical composition according to claim 1, wherein said autoimmune disease is an autoimmune degenerative disease of blood vessels.

5. A pharmaceutical composition according to claim 1, wherein said autoimmune disease is systemic lupus erythematodes.

6. A pharmaceutical composition according to claim 1, wherein said autoimmune disease is glomerulonephritis.

7. A pharmaceutical composition according to claim 1, wherein said autoimmune disease is an autoimmune degenerative disease of joints.

8. A pharmaceutical composition according to any one of claims 1 to 7, formulated for concomitant use with a glucocorticoid.

9. Use of toremifene, or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for the treatment of an autoimmune disease.

10. A use according to claim 9, wherein said autoimmune disease is an autoimmune degenerative disease of kidneys.

11. A use according to claim 9, wherein said autoimmune disease is an autoimmune degenerative disease of salivary glands.

12. A use according to claim 9, wherein said autoimmune disease is an autoimmune degenerative disease of blood vessels.

13. A use according to claim 9, wherein said autoimmune disease is systemic lupus erythematodes.

14. A use according to claim 9, wherein said autoimmune disease is glomerulonephritis.

15. A use according to claim 9, wherein said autoimmune disease is an autoimmune degenerative disease of joints.

16. A use according to any one of claims 9 to 15, wherein the medicament is formulated for concomitant use with a glucocorticoid.