CA2137912A1 - Combinatorial polypeptide antigens - Google Patents
Combinatorial polypeptide antigensInfo
- Publication number
- CA2137912A1 CA2137912A1 CA002137912A CA2137912A CA2137912A1 CA 2137912 A1 CA2137912 A1 CA 2137912A1 CA 002137912 A CA002137912 A CA 002137912A CA 2137912 A CA2137912 A CA 2137912A CA 2137912 A1 CA2137912 A1 CA 2137912A1
- Authority
- CA
- Canada
- Prior art keywords
- amino acid
- variants
- polypeptide antigens
- protein
- type
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000427 antigen Substances 0.000 title abstract 4
- 102000036639 antigens Human genes 0.000 title abstract 4
- 108091007433 antigens Proteins 0.000 title abstract 4
- 229920001184 polypeptide Polymers 0.000 title abstract 4
- 102000004196 processed proteins & peptides Human genes 0.000 title abstract 4
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 4
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 7
- 150000001413 amino acids Chemical class 0.000 abstract 4
- 102000004169 proteins and genes Human genes 0.000 abstract 3
- 108090000623 proteins and genes Proteins 0.000 abstract 3
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16111—Human Immunodeficiency Virus, HIV concerning HIV env
- C12N2740/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Virology (AREA)
- Peptides Or Proteins (AREA)
Abstract
This invention pertains to a set of polypeptide antigens having amino acid sequences derived from amino acid sequences of a population of variants of a protein, or a portion thereof, and to methods of producing the set of polypeptide antigens. In general, the method comprises (i) selecting a protein, or a portion thereof, which exhibits a population of N variants, represented by the formula A1A2A3...A n-2A n-1A n, where A n is an amino acid occurring at amino acid position n of the protein, or portion thereof; (ii) determining the number of times O n aa each type of amino acid occurs at each amino acid position n in the N
variants; (iii) calculating the frequency of occurrence (O n aa/N)n of each type of amino acid at each amino acid position n in the N variants; and (iv) generating a set of polypeptide antigens having amino acid sequences represented substantially by the formula A'1A'2A'3...A'n-2A'n-1A'n, where A'n is defined as an amino acid type which occurs at greater than a selected frequency at the corresponding amino acid position in the N variants.
variants; (iii) calculating the frequency of occurrence (O n aa/N)n of each type of amino acid at each amino acid position n in the N variants; and (iv) generating a set of polypeptide antigens having amino acid sequences represented substantially by the formula A'1A'2A'3...A'n-2A'n-1A'n, where A'n is defined as an amino acid type which occurs at greater than a selected frequency at the corresponding amino acid position in the N variants.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US90012392A | 1992-06-18 | 1992-06-18 | |
| US900,123 | 1992-06-18 | ||
| PCT/US1993/005899 WO1994000151A1 (en) | 1992-06-18 | 1993-06-18 | Combinatorial polypeptide antigens |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2137912A1 true CA2137912A1 (en) | 1994-01-06 |
| CA2137912C CA2137912C (en) | 2007-05-08 |
Family
ID=38042983
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002137912A Expired - Fee Related CA2137912C (en) | 1992-06-18 | 1993-06-18 | Combinatorial polypeptide antigens |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA2137912C (en) |
-
1993
- 1993-06-18 CA CA002137912A patent/CA2137912C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CA2137912C (en) | 2007-05-08 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |