CA2110019A1 - Immunoassays for determining vitamin b12, and reagents and kits therefor - Google Patents
Immunoassays for determining vitamin b12, and reagents and kits thereforInfo
- Publication number
- CA2110019A1 CA2110019A1 CA 2110019 CA2110019A CA2110019A1 CA 2110019 A1 CA2110019 A1 CA 2110019A1 CA 2110019 CA2110019 CA 2110019 CA 2110019 A CA2110019 A CA 2110019A CA 2110019 A1 CA2110019 A1 CA 2110019A1
- Authority
- CA
- Canada
- Prior art keywords
- immunoassays
- reagents
- vitamin
- kits therefor
- determining vitamin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000003018 immunoassay Methods 0.000 title abstract 2
- 239000003153 chemical reaction reagent Substances 0.000 title 1
- RMRCNWBMXRMIRW-WYVZQNDMSA-L vitamin b12 Chemical compound N([C@@H]([C@@]1(C)[C@@](C)(CC(N)=O)[C@H](CCC(N)=O)\C(N1[Co+]C#N)=C(/C)\C1=N\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NCC(C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO RMRCNWBMXRMIRW-WYVZQNDMSA-L 0.000 title 1
- 229940029329 intrinsic factor Drugs 0.000 abstract 4
- 229930003779 Vitamin B12 Natural products 0.000 abstract 2
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 abstract 2
- 239000011715 vitamin B12 Substances 0.000 abstract 2
- 235000019163 vitamin B12 Nutrition 0.000 abstract 2
- 238000003556 assay Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
Methods are described for the development of monoclonal antibodies to intrinsic factor and the application of said antibodies in the development of B12 immunoassays. In particular, antibodies are described that are capable of binding to intrinsic factor only in the absence of vitamin B12, and that are released from binding in the presence, and upon the binding, of vitamin B12 to intrinsic factor. Assays may be formatted such that either antibody or intrinsic factor is immobilized or labeled.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/070,099 US6942977B1 (en) | 1991-04-09 | 1993-05-28 | Immunoassays for determining vitamin b12, and reagents and kits therefor |
| US070,099 | 1993-05-28 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2110019A1 true CA2110019A1 (en) | 1994-11-29 |
| CA2110019C CA2110019C (en) | 2006-01-24 |
Family
ID=22093115
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA 2110019 Expired - Fee Related CA2110019C (en) | 1993-05-28 | 1993-11-25 | Immunoassays for determining vitamin b12, and reagents and kits therefor |
Country Status (2)
| Country | Link |
|---|---|
| JP (1) | JP3433245B2 (en) |
| CA (1) | CA2110019C (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005074461A2 (en) * | 2004-01-16 | 2005-08-18 | Beckman Coulter, Inc. | Kits and methods for autoantibody detection |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023239935A1 (en) * | 2022-06-10 | 2023-12-14 | Xeragenx Llc | Detection of gastric intrinsic factor (if) in fluid samples |
-
1993
- 1993-11-25 CA CA 2110019 patent/CA2110019C/en not_active Expired - Fee Related
-
1994
- 1994-05-27 JP JP13823094A patent/JP3433245B2/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005074461A2 (en) * | 2004-01-16 | 2005-08-18 | Beckman Coulter, Inc. | Kits and methods for autoantibody detection |
| WO2005074461A3 (en) * | 2004-01-16 | 2006-10-19 | Beckman Coulter Inc | Kits and methods for autoantibody detection |
Also Published As
| Publication number | Publication date |
|---|---|
| JP3433245B2 (en) | 2003-08-04 |
| CA2110019C (en) | 2006-01-24 |
| JPH0712811A (en) | 1995-01-17 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |