CA2102237A1 - Method and Apparatus for Immobilized Enzyme Reactions - Google Patents

Method and Apparatus for Immobilized Enzyme Reactions

Info

Publication number
CA2102237A1
CA2102237A1 CA2102237A CA2102237A CA2102237A1 CA 2102237 A1 CA2102237 A1 CA 2102237A1 CA 2102237 A CA2102237 A CA 2102237A CA 2102237 A CA2102237 A CA 2102237A CA 2102237 A1 CA2102237 A1 CA 2102237A1
Authority
CA
Canada
Prior art keywords
pores
particles
sets
immobilized enzyme
immobilized
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA2102237A
Other languages
French (fr)
Other versions
CA2102237C (en
Inventor
Noubar B. Afeyan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems LLC
Original Assignee
Noubar B. Afeyan
Perseptive Biosystems, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Noubar B. Afeyan, Perseptive Biosystems, Inc. filed Critical Noubar B. Afeyan
Publication of CA2102237A1 publication Critical patent/CA2102237A1/en
Application granted granted Critical
Publication of CA2102237C publication Critical patent/CA2102237C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/16—Particles; Beads; Granular material; Encapsulation

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Perfusive matrices (10) useful in immobilized enzyme reactors (IMERs) are disclosed. The matrices define first and second interconnected sets of pores and an intraparticle high surface area (18) capable of immobilizing enzymes, in fluid communication with the second set of pores. The first and second sets of pores are embodied, for example, as the interstices (12) among particles and throughpores (14) within the particles. The particles also contain diffusive transport pores (16). The dimensions of the first and second sets of pores are such that, at fluid flow rates and pressure drops practical for immobilized enzyme reactions, convec-tive flow occurs in both pore sets, and the convective flow rate exceeds the rate of diffusion of the substrate and product to and from immobilized enzymes within the particles. This approach allows rapid enzyme loading and substrate conversion reactions at optimal catalytic flow rates.
CA002102237A 1991-04-19 1992-04-09 Method and apparatus for immobilized enzyme reactions Expired - Fee Related CA2102237C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US68863891A 1991-04-19 1991-04-19
US688,638 1991-04-19

Publications (2)

Publication Number Publication Date
CA2102237A1 true CA2102237A1 (en) 1992-10-20
CA2102237C CA2102237C (en) 1995-12-12

Family

ID=24765170

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002102237A Expired - Fee Related CA2102237C (en) 1991-04-19 1992-04-09 Method and apparatus for immobilized enzyme reactions

Country Status (5)

Country Link
EP (1) EP0580761A4 (en)
JP (1) JPH06507313A (en)
AU (1) AU1917292A (en)
CA (1) CA2102237C (en)
WO (1) WO1992018636A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69319593T2 (en) * 1992-03-27 1998-12-17 Perseptive Biosystems Inc FAST HIGH-SENSITIVE IMMUNANALYTIC FLOW DETECTION SYSTEM
DE69835275T2 (en) 1997-01-07 2007-07-19 Kaneka Corp. ABSORBENT FOR CLEANING BODY FLUIDS
FR2798137A1 (en) * 1999-09-07 2001-03-09 Bonneau Marguerite Gabr Calone GENERATING APPARATUS FOR OXYGENIC CHEMICAL RADICALS AND INDUSTRIAL APPLICATIONS
US8715982B2 (en) 2005-03-08 2014-05-06 Agency For Science, Technology And Research Immobilised enzymes
EP1931776B1 (en) * 2005-10-05 2011-09-28 Kao Corporation Method for producing a fatty acid by use of an immobilized enzyme
CN110205235A (en) * 2019-05-05 2019-09-06 张克林 A kind of high-volume Rapid manufacturing device of high of microalgae Immobilized culture glueballs

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3841971A (en) * 1973-02-16 1974-10-15 Corning Glass Works Synergistic enzymes adsorbed within porous inorganic carriers
US4070348A (en) * 1973-07-25 1978-01-24 Rohm Gmbh Water-swellable, bead copolymer
US4001085A (en) * 1973-09-10 1977-01-04 Owens-Illinois, Inc. Immobilization of enzymes on an inorganic matrix
US3982997A (en) * 1974-09-18 1976-09-28 Corning Glass Works Immobilized glucose isomerase
GB1525022A (en) * 1975-05-21 1978-09-20 Beecham Group Ltd Cell culture method
AU630427B2 (en) * 1989-07-06 1992-10-29 Perseptive Biosystems, Inc. Perfusive chromatography
US5262320A (en) * 1990-06-18 1993-11-16 Massachusetts Institute Of Technology Cell-culturing apparatus and method employing a macroporous support

Also Published As

Publication number Publication date
JPH06507313A (en) 1994-08-25
AU1917292A (en) 1992-11-17
WO1992018636A1 (en) 1992-10-29
EP0580761A4 (en) 1995-11-22
CA2102237C (en) 1995-12-12
EP0580761A1 (en) 1994-02-02

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