CA2099210A1 - Enhancement of musculature in animals - Google Patents

Enhancement of musculature in animals

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Publication number
CA2099210A1
CA2099210A1 CA002099210A CA2099210A CA2099210A1 CA 2099210 A1 CA2099210 A1 CA 2099210A1 CA 002099210 A CA002099210 A CA 002099210A CA 2099210 A CA2099210 A CA 2099210A CA 2099210 A1 CA2099210 A1 CA 2099210A1
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Prior art keywords
ski
dna
protein
muscle
construct
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CA002099210A
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French (fr)
Inventor
Stephen H. Hughes
Pramod Sutrave
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US Department of Commerce
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Individual
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Priority claimed from CA002059137A external-priority patent/CA2059137C/en
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Abstract

ABSTRACT
The present invention relates to DNA segments encoding chicken c-ski protein, to DNA constructs comprising the DNA
segments and to cells transformed therewith. The present invention further relates to transgenic animals having increased muscle size. In addition, the present invention relates to methods of stimulating muscle growth and preventing degeneration of muscle, and to methods of treating muscle degenerative diseases.

Description

W09i/00~87 ~ 0 ~ 9 2 1 0 PCT/U590/037~9 EN~ANCEXENT 0~ H~SC~LATURE IN ANIHALS

BAC~GROUND OF TH~ INnn~NTION

Field of the Invention The present invention relates the c-ski gene.
In particular, the present invention relates to DNA segments encoding chicken c-s~i protein, to DNA constructs comprising the DNA segments and to cells transformed therewith. ~he present invention further relates to animals having increased muscle size.

Background Information Viruses that contain the v-ski oncogene are not only capable of causing morphological transformation in vitro, but also can induce myogenic differentiation (Stavnezer et al., 1981, J. Virol. 39, 920-934; Li et al., 1986, J. Virol.
57, 1065-1072; Stavnezer et al., 1986, J. Virol.
57, 1073-1083; Colmenares and Stavnezer, 1989, Cell 59, 293-303). Viruses that carry and express 2S c-sk~ cD~As also induce foci and myogenic differentiation (Sutrave et al., 1990, Mol. Cell.
Biol. 10, 3137-3144). This suggests the pos-sibility that the ski oncogene is bifunctional since the two known functions of sk~, trans-formation and differentiation, would appear to becontradictory properties. Using a v-ski probe, genomic clones for c-ski have been isolated and partially sequenced (stavnezer et al., 1989, Mol.

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WO91/00287 ~ 9~,1D ~CT/USso/037~9 Cell. Biol. 9, 4038-4045). Comparisons of the properties of two forms of c-ski that are related by alternative splicing, and of several v-sXi and c-ski deletion mutants have shown that the portions of ski required for transformation and differentiation are quite similar. These results suggest that the ability of c-ski and v-ski to cause transformation and induce differentiation / may be related aspects of a single property of ski rather than two separate functions.
Relatively little is known about the biochemical functions of the ski proteins. All of the biologically active forms of c-ski and v-ski that have been studied are localized primarily in lS the nucleus (Bar~as et al., 1986, Virology 151, 131-138; Sutrave et al., 1990, Mol. Cell. Biol.
10, 3137-3144). When the c-ski proteins are overexpressed in chicken cells, different f orms of c-ski dif f er in their subnuclear localization;
however, the significance of these differences, if any, is as yet unclear. When chromatin condenses for cell division, the over-expressed c-ski - proteins are associated with the condensed chromatin tSutrave et al., 1990, Mol. Cell. Biol.
10, 3137-3144). ~3iochemical studies have also shown that at least one for~ of c-ski can bind to DNA in the presence of other proteins (Nagase et al., 1990, Nucl. Acids ~es. 18, 337-343).
None of the available data make it possible to infer the normal function of c-ski either in terms of its role in growth and development (if any) or to have any direct insight into its mode of action. --' ' ; ' 2 ~ ~ ~ 2 ~ ~ PCT/US90/03729 SUMMARY OF THE I~VENTION

It is an object of the present invention to provide an isolated and characterized c-ski cDNA.
It is another object of the present invention to provide a gene that increases the muscle size in animals.
It is another object of the present invention to provide domestic livestock with increased muscle size and decreased fat tissue.
It is a further object of the present invention to provide a treatment for patients suffering from serious muscle injury or muscle degenerative diseases.
Various other objects and advantages o2 the present invention will be apparent from the drawings and the following description of the ~nvention.
In one embodiment, the present invention relates to a DNA segment encoding a chicken c-ski protein or a DNA fragment complementary to said segment.
In another embodiment, the present invention relates to a DNA construct comprising a DNA
segment encoding a chicken c-ski protein and a vector. In a further embodiment, the present invention relates to a DNA construct comprising a DNA segment encoding a truncated chicken c-s~i protein having the function of c-ski and a vector.
T~e present invention also relates to host cells stably trans2Ormed with either to of the two DNA
constructs described above, in a manner allowing expression of the protein encoded in the construct.
In yet another embodiment, the present invention relates to a animal having increased muscle size, all of whose cells contain a DNA

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wO91/00287 ~ ~ g ~ 2 ~ ~ PCT/usso/0372s construct comprising a DNA ~egment encoding a ski protein and a vector, introduced into the animal, or an ancestor of the animal. The DNA segment may encode the entire protein or a truncated version thereof.
In further embodiment, the present invention relates to an animal having increased muscle size, all of whose cells contain a DNA construct comprising a DNA segment encoding a truncated ski protein having the function of ski and a vector, ~ntroduced into the animal, or an ancestor of the animal.
In another embodiment, the present invention relates to a method of stimulating muscle growth or preventing muscle degeneration comprising delivering a DNA construct of the present invention to the muscle under conditions such that the protein of the construct is expressed and muscle qrowth induced.
In a further embodiment, the present invention relates to a method of treating a muscle degenerative disease comprising delivering a DNA
construct of the present invention to the effected muscle under conditions such that the protein of the construct is expressed and treatment effected.

BRIEP DESCRIPTION OF l~IE DRAWINGS

Figure 1. The Structure of the c-ski cDNA
clones.
The lengths of the cDNAs are drawn to scale and the restriction sites indicated. v-ski is shown for comparison. The dotted boxes in v-ski represent the gag region of the gag-ski fusion in the acutely transforming virus SXV. A, ~, C and D
represent the regions used for generating single-strand probes for Sl nuclease protection analysis.

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The num~ers next to the arrows correspond to the exon number.

Figure 2. Complete coding sequence and the potential coding region of a cDNA of the F829 type.
This assumes that the 5' end sequences of the FB29 are similar to the 5' ends of the FB28 and CEL
clones. t indicates the site where FB28 and CEL
diverge. The 25 bases found only in the CEL
clones are not shown. ~ indicates the boundaries of v-ski. The exon boundaries are numbered and the alternately spliced exons are boxed. The single base and amino acid change between c-ski and v-ski is also boxed with a dashed line. The translation termination codon is boxed in thick lines. The potential polyadenylation signals are densely underlined. The AT rich region containing A m A sequences that might be involved in mRNA `
stability is underlined with dashed lines.

Figure 3. Diagrammatic illustration of the alternate mRNAs generated for c-ski locus as deduced from the cDNA sequence analysis.
The exons are not drawn to scale. The c-ski mRNAs are shown in relation to v-ski. The dark areas are noncoding regions while the open boxes are the protein coding regions of the cDNA. The dotted boxes on both ends of v-ski are the gag regions of the gag-ski fusion in transforming ski viruses.
The relative positions of the putative translational-initiation codon and the translation termination codons are also shown.

,1 wosl/00287 ~ PCT/USso/03729 Flgure 4. S1 nuclease protection analysis of total RNA. Fiqure 4A shows the uniformly labeled single-strand probes used for hybridization are shown schematically below each picture, the thick lines represent the cDNA
sequence while the thin lines represent M13 seguence. ~he overall lenyth of probes and expected lengths of protected fragments are also shown. The RNA hybridization is indicated at the top of each lane. The numbers (8, 10, 12, lS or 17) above the lane indicates the age of the embryo from which the RNA was prepared. Figure 4B
showsprobe A (see Figure 1) contains a RpnI-HindIII
fragment of the F827/29 type. This probe produced a fragment of 645 base pairs (bp) and two smaller fragments of 262 and 272 bp, shown by arrOWs.Figure 4C
showsProbe B (see Figure 1~ contains a ~pnI-~indIII
fragment of the FB28 type. This probe produced a fragment of 534 bp as shown by the arrows. -Smaller fragments were not detected. Figu-e 4D
showsProbe C (see Figure 1) contains a 497-bp ~indIII fragment of the FB27 type linked to M13 sequences. The probe yielded a 497-bp fragment and two smaller fragments of 243/254. Only the 479-bp fragment is marked by an arrow. Figure 4E
showsProbe D (see Figure 1) is 1116 bp in length containing a ~indIII fragment of FB28/29 type.
Probe D produced a 799-bp fragment which is mar~ed by an arrow.

Figure 5. Seguence homology between c-ski and the pl9 region of gag from avian leukosis virus.
The c-ski seguences are from positions 218 to 242 and the pl9 seguence of gag region are from positions 633 to 658. The homologous regions are 35 boxed.

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WO91/00287 2 Q ~ ~ ~, 1 0 PCT/US90/03729 , Figure 6. The c-ski expression cassette.
The PvuI to Nrul segment shown in the drawing was isolated by gel electrophoresis following double digestion of the plasmid. The linear DNA was used to create the transgenic mice by microinjection of fertilized eggs.

Figure 7. A transgenic mouse that expresses c-ski and a normal litter mate.
The c-sk~ transgene appears to segregate normally in crosses. The photograph shows a heterozygous mouse that displays the muscular phenotype (foreground) and a DNA-negative litter mate.
Double blind DNA analyses confirmed that the muscular phenotype segregates with the transgene.

Figure 8. Northern transfer analysis of transgene expression.
Panel A shows the analysis of RNA isolated from various tissues of a mouse of the line TG 8S66.
The upper part of the panel shows an autoradiogram after hybridization with chicken c-s~i. The expected position of migration of the c-ski message appropriately transcribed from the transgene is 2.5 kb. The position corresponding to 2.5 kb is marked (ski). The lower panel shows an autoradiogram from the same filter following hybridization to a chicken ~-actin cDNA. The ~-actin cDNA will hybridize not only with ~-actin mRNA but also with other actin messages. The expected position of migration of both ~-actin and c-actin mRNAs are indicated on the right of the panel.
Panel B. The autoradiograms shown in panel ~ are similar to those shown in panel A except that the RNAs derive from three other transgenic lines.
The lines used to prepare the RNAs are indicated ' ' -~$
WO 91/00287 ~ ~ J ^~ 2 ~ PCI'/US90/03729 .~ _ at the top of the figure. The filters shown in panel B were done at the same time; those in panel A were done on a different day.

Figure 9. RNase protection of RNA from the transgene.
The top of the figure shows an autoradiogram of the gel. The first lane contains the antisense RNA probe, without RNase digestion. The next two lanes show the results oS digestion following hybridization of the probe either without added RNA or with tRNA. The next eight lanes show the results of hybridization to RNA isolated either from the hearts (heart) or the skeletal muscle (SK
muscle) of the four transgenic lines. The next lanes show the results of hybridizing the probe to RNA from skeletal muscle of a mouse that does not carry the transgene (control SX muscle). The last lane contains molecular weight markers.
Below the autoradiogram is a diagram that shows a drawing of the MSV LTR c-ski expression cassette in relation to the antisense RNA probe. The T7 transcript begins in the middle of the c-ski coding region and goes entirely through the MSV
L~R into adjacent sequences that derive from 25 pBR322 (marked p~R). If the transcripts deriving from the transgene initiate appropriately, then a fragment of 984 bases should be protected.

Figure 10. chicken c-ski protein expression in transgenic mice.
Extracts were made from the liver (Liv) or skeletal muscle (Sk.M) of control mice (control) or mice carrying and expressing the c-ski transgene. The positions of migration of radioactively labeled molecular weight standards are shown to the left of the figure.

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Figure 11. Cross sections made precisely through the middle of the plantaris muscle: (a) from control mouse and (b) from a mouse of line TG
8566. ~oth illustrations are at the same mngnification, the size marker is 200 ~. (c) Higher power illustration from the plantaris of control and (d) from the plantaris of an affected mouse. Size markers in (c) and (d) are 100 ~.
.
Figure 12. Distribution of fiber diameters in selected muscles from normal and transgenic Mice Panel A. The diaphragm appears normal in transgenic mice that express c-s~i. A diaphragm from a mouse that has the muscular phenotype (TG
8566) and a diaphragm from a normal control mouse were sectioned and the number of individual muscle fibers of a given cross-sectional area tallied.
Panel B. The anterior tibial muscle is grossly enlarged in mice from the line TG 8566.
Transverse sections were prepared from both a transgenic mouse and a control mouse. The number of fibers of each given cross-sectional area were tallied. This muscle is composed of two distinct types of fibers, some of which are smaller, others larger, than the fibers found in the controls (see also Figure 11).

Figure 13. Transgene expression in specific muscles from TG 8566.
20 ~g of total RNA was fractionated by electrophoresis and transferred to nitrocellulose membranes. The transfers were first probed with chicken c-s~i, then the filters were stripped and reprobed with a chicken ~-actin cDNA (Cleveland et al., 1980, Cell 20, 95-105). The RNA was isolated from the diaphragm, the soleus, or from bulk .:
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WO 91/00287 7 ~ 3 9 ~ ~ IJ PCI/US90/03~29 - lr _ skeletal muscle (sk muscle).
Flgure 14. Immunofluorescence staining of sections made through the middle of the Rhomboideus capitis muscle of an affected mouse.
(a) staining with monoclonal antibody NOQ7 5 4D, specific for slow MHC. Slow fibers are not hyper-trophied. (~) staining with monoclonal anti~ody - SC 711 specific for IIa MHC. lIa fibers are not hypertrophied. (c) is with monoclonal antibody 2G3 which reacts with all fast MHC isoforms. All hypertrophied fibers stain with this antibody.
(d) is with mla BF-F3 specific for IIb MHC. Many, but not all, hypertrophied fibers stain.
lS Magnification x 230.

DETAILED DESCRIPTION OF THE INVENTION
The present invention relates to a DNA
segment encoding all, or a unique portion, of a chicken c-ski protein. The DNA segment may encode one of several chicken c-ski proteins, for example, F829, FB28 and F827. A "unique portion"
as used herein is defined as consisting of at least five (or six) amino acids or correspondingly, at least 15 (or 18) nucleotides.
The invention also relates to DNA constructs containing such DNA segments and to cells transformed therewith.
The present invention relates to DNA
segments that encode the amino acid seguence of exon 6 given in Figure 1 or the amino acid sequence of exon 7 given in Figure 1. The present invention also relates to DNA segments that in addition to exon 7 further comprise at least four exons selected from the group consisting of: exon 3S 1, exon 2, exon 3, exon 4, exon 5 or exon 6, given in Figure 1. Examples of such DNA segments WOgl/0028~ ~ 0 3 ~ 2 ~ ~ PCT/US90/03729 include FB29, F~28, and FB27.
DNA segments to which the invention relates also include those encoding substantially the same proteins as those encoded in the exons of Figure 1 which includes, for example, allelic forms of the F~gure 1 amino acid sequences. The invention also relates to DNA fragments complementary to such sequences. A unique portion of the DNA segment or the complementary fragment thereof of the present ~nvention can be used as probes for detecting the presence of its complementary strand in a DNA or RNA sample.
The present invention further relates to DNA
constructs and to host cells transformed therewith. In one embodiment, the DNA constructs of the present invention comprise a DNA segment -encoding a c-ski protein of the present invention and a vector, for example, pMEX neo. In another embodiment, the DNA constructs comprise a DNA
segment encoding a truncated c-ski protein having the function of c-ski (such as, for example, ~FB29) and a vector (for example, pMEX neo). The DNA construct is suitable for transforming host cells. The host cells can be procaryotic or, preferably, eucaryotic (such as, mammalian).
The present invention further relates to animals, such as, for example, domestic livestock, having increased muscle size. The development of such strains of domestic livestock with increased muscles continues to be a major goal of conventional breeding schemes. (Domestic livestock as used herein refers to animals bred for their meat, such as, for example, pigs, chickens, turkeys, ducks, sheep, cows and fish, particularly, trout and catfish).
Introduction of various genes into the germ lines of mice and of some types of domestic - , . .
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WO91/00287 ~ 2 ~ ~ PCTtUS90/03729 _ 12 -livestock ls relatively routine to those sk~lled in the art. The present inventors have produced mice having increased muscle size by introducing a DNA construct comprising ~FB29 and the pMEX neo vector into fertilized eggs. Resulting founder mice and their offspring have the DNA construct in all their cells, somatic and germ.
Introduction of DNA constructs encoding a ski protein (such as, for example a c-ski protein), into fertilized eggs of animals (such as, by microinjection) results in strains of animals having increased muscle development and decreased fatty tissue. As one skilled in the art will appreciate, the animals with increased muscle size of t~e present invention can also be produced using DNA encoding a ski protein from various species (chicken being just one such example).
Furthermore, animals of the present invention can be produced using a DNA construct encoding protiens related to ski, such as, for example, sno gene.
The DNA segment A F829 generated by a frameshift mutation results in a truncated protein. However, as one skilled in the art will appreciate, the transgenic animals of the present invention can also be generated by DNA constructs containing DNA segments encoding a full length ski protein, a portion of a ski protein, such as, one or two exons or a biological active deletion derivative, such as, for example, v-ski, which represents a truncated c-ski fused to a viral protein. Further, it is also recognized that the selective expression of the protein in muscle tissue may result from DNA constructs created in 3s vectors other than pMEX neo.
~ he present invention also relates to a method of stimulating muscle growth and preventing muscle wosl/ooz87 2 ~ ~ ~ 2 ~ O PCT/US90/03729 degeneration in an animal, such as for example, a hu~an. A possible treatment for injuries re6ulting in loss of mu~cle tissue and neurological injuries resulting in degeneration of the muscle would be to stimulate muscle growth.
In the case of lose of muscle this would involve stinulating regrowth of the tissue. Whereas in the case of neurological injuries, the muscle growth would need to be rendered independent of the missing nerve stimulus. According to the present invention, muscle growth could be stimulated by delivering a DNA construct encoding a ski protein to the muscle tissue under conditions such that the protein encoded in the construct is expressed. The construct can be targeted and delivered to the muscle using standard methods known to those s~illed in the art.
The present invention further relates to a method of treating a muscle degenerative disease such as, for example, muscular dystrophy and amyotrophic lateral sclerosis (also known as Lou Gehrig disease). Treatment would comprise delivering a DNA construct of the present invention to the effected muscle under conditions such that the protein encoded in the construct is expressed and treatment effected.

Examples Screening of cDNA ~ibrary Two chicken cDNA libraries were screened with a v-ski probe using standard protocols (Maniatis et al., 1982, Molecular Cloning. A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring 8arbor, NY). One library was made from poly A
mRNA isolated form the body wall of 10-day embryos and the other from mRNA isolated from AEV-.
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-WO91/00287 ~ 9 ~ PCT/US90/03729 _ 14 -transSormed chlcken erythroblasts. Four distinct c-sk~ c~NAs were isolated; three were from the body wall library (these cloned were designated FB27, FB28 and FB29) and one cDNA clone from the erythroblast library (designated CEL). These cDNAs included sequences extending both 5' and 3' of the portion of ski present in the virus. The cDNAs demonstrate that v-ski derives from a single cellular gene and suggest that multiple c-s*~
~RNAs, encoding distinct ski proteins, are produced from the c-ski locus by alternate splicing (Leff et al., 1986, Ann. Rev. 8iochem.
55, 1091-1117), adding to a growing list of oncogenes known to produce multiple mRNAs in this fashion (Ben-Neviah et al., 1986, Cell 44, 577-586; Levv et al., 1987, Mol. Cell. Biol. 7, 4142-4145; Martinez et al., 1987, Science 237, 411-415;
McGrath et al., 1983, Nature 304, 501-506).

Structure of t`he cDNA clones The structure of all of the c-ski cDNA clones that have been characterized by DNA sequencing (Sanger et al., 1977, Proc. Natl. Acad. Sci. USA
74, 5463-5467) and their relationships to v-ski are presented in Figure 1.
Only FB28 and CEL have sequences 5' of the v-ski seguences; both F827 and FB29 are truncated at the 5' end. Assuming that the missing seguences at the 5' end of F829 are similar to those in FB28 and CE~, a composite nucleotide sequence and the deduced amino acid sequence for a cDN~ of the FB29 ..... ....... ..... .. . _ ., , _,, __ _ . .. _ ., type is shown in Figure 2. The first ATG with a .
su~stantial downstream open reading frame is located at nucleotide position 168. Upstream of this ATG no reading frames are open, suggesting that these sequences represent the 5' untranslated region.

W09l/00287 PCT/US90/03729 2~2~.0 Based on the cDNA sequence analysis and comparisons wit~ the positions of the splice donor and acceptor sites known from the genomic sequence (Stavnezer et al., 1989, Mol. Cell. Biol. 9, 4038-4045), exon boundaries have been derived (see Figure 3). As seen in the figure, c-ski sequences are distributed over seven exons. FB29 contains all seven exons; FB28 and FB27 lack exon 2 and exon 6, respectively. This differential splicing affects the protein coding potential of the three cDNAs.
Dlfferential splicing of exon 2 deletes 37 amino acids without affecting the coding potential of the open reading frame downstream.
Differential splicing of exon 6, however, affects the coding potential of exon 7. If exon 5 is spliced to exon 7 (seen in FB27 cDNA?, a translation termination codon is generated at the splice junction and exon 7 becomes~a noncoding exon. However, if exon 5 is spliced to exon 6 and exon 6 to exon 7, as in FB28/29, then the open reading frame continues in exon 7 for 417 nucleotides encoding an additional 129 amino acids.
Assuming that the missing 5' ends of the FB29 and FB27 mRNAs contain sequences identical to those present in CEL and FB28, then translation of ~RNAs corresponding to the three different types of cDNAs would lead to the generation of three proteins, one containing 750 amino acids (rom FB29), the second 713 amino acids (from FB28), and a third protein containing 510 amino acids (from ~-FB27).
The CEL clone is missing 3' sequences. cDNAs that derive from t~e body wall (FB) library have long 3' untranslated regions that contain a 95-base pair (bp) AT-rich region from nucleotide 2803 - - . ..

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to 2898. Within this region there are two copies of a sequence ATTTA that has been implicated ln mRNA destabilization in a variety of transiently induced mRNAs including c-myc, interferon, c-jun, and c-fos (Meijlink et al., 1985, Proc. Natl.
Acad. Sci. USA 82 , 4987-4991; Ryder et al., 1985, Proc. Natl. Acad. Sci. USA 85, 1487-1491; Shaw et al., 1986, Cell 46, 659-667). Addition or deletion of these sequences have also been shown to affect the transformation potential of c-fos ~Meijlink et al., 1985, Proc. Natl. Acad. Sci. USA
82, 4987-4991).
The c-ski cDNAs contain two potential poly(A) signals (AATAAA) located at positions 3348 and 4167. Althouqh all three clones isolated from the FB library end at the same position, none has a poly(A) tail; therefore, it is likely that the 3' ends of the c-ski mRNAs are not contained in these clones. The 4.2-kb cDNAs that were isolated and I characterized are smaller than the 5.7 -8.0 and 10.0-kb mRNAs detected by Northern transfer _analysis (~i et al., 1986, J. Virol. 57, 1065-1072). This discrepancy has not been explained, however, it is suggested that the clones isolated so far, which lack poly(A) tails, also lack sequences from the 3' ends of the mRNAs.

The 5' End o$ c-ski mRNA ( s ) Sequence comparisons at the 5' end reveals that both FB28 and CEL are colinear up to a position 89 bp upstream of the putative translational init$ation ATG codon. FB28 has an additional 76-bp while CEL has 25 bp that are d$fferent from those found in FB28. This region where the two sequences diverge has been compared with sequences from genomic clones (Stavnezer et al., 1989, Mol. Cell. Biol. 9, 4038-4045) and the wos1/ooz87 ~ ~ J ~ O PCT/USgO/03729 upstream sequences in the FB28 are colinear with the genomic sequences.
Examination of the sequences in the genomic DNA at the point of divergence of CEL and F828 does not reveal consensus sequences for donor or acceptor splice sites. In order to confirm the authenticity of the clones, Sl nuclease protection analysis was carried out. ~he results demonstrated that the seguences present in FB28 are expressed as mRNA in normal embryos. Similar Sl analyses have provided no evidence for the presence in mRNA of the 25 bp at the extreme 5' end of the CEL clone. It is suggested that the first 25 bases of the CEL clone are the result of a cloning artefact, and that the seguences found in the F828 clone are expressed in c-ski mRNA(s).
In an attempt to determine how much of the 5' untranslated region of the c-ski mRNA(s) are contained in FB28, primer extension analysis was carried out. Copying the 5' segment present in FB28 should give an extension product of about 280 bases. However, a primer extension product of 220 bases was seen. Sl analyses data have shown that this segment is expressed in RNA. It is possible that the observed primer extension product is the result of premature termination; however, it is also possible that there are multiple 5' ends for the c-ski mRNAs.

Organization of the Internal Exons Sequence comparisons in the central portion of FB27, FB28 and FB29 and CEL cDNAs reveal that FB28 lacks a small region of lll bp (exon 2) from 1079-ll9l that would eliminate 37 amino acids. The genomic sequence (Stavnezer et al., 1989, Mol.
Cell. Biol. 9, 4038-4045~ in the corresponding region reveals the existence of consensus splice , .
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WO91/00287 1~8~ '~ PCT/USsO/03729 donor and acceptor sequences at the boundaries of the deletion suggesting t~at the cDNA derives from a differentially spliced mR~TA.
In order to confirm the existence of mRNAs of the FB28 and of the FB27/29 type, S1 analysis was carried out on total RNA. Total RNA was isolated from 8, 10, 12, 15 and 17-d~y-old chicken embryos using standard protocols (Chirgwin et al., 1979, Biochemistry 18, S294-5299). Approximately 20 to 30 ~g of total RNA was used for nuclear S1 analysis using standard procedures (Maniatis e~
al., 1982, Molecular Cloning. A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY).
A uniformly labeled single-strande`d probe spanning the region between the ~pnI and ~i~dIII
sites of FB29/27 or FB28 (probes A and B of Figure 1) was hybridized with total cellular RNA. As shown in Figure 4B, hybridization to mRNA and subsequent S1 digestion of a probe derived from FB29 produced protected fragments of 645 bp indicating hybridization to mRNA of the FB27/29 type and the 262/272 bp fragments expected if the probe hybridized with mRNA of FB28 type. FB28 2S (probe B) protected a fragment of 534 bp ~see Figure 4C). Smaller fragments from the hybridization of the FB28 probe to mRNAs of the FB27/29 type were not observed; however, Sl does not always cleave a DNA probe efficiently opposite a looped out region of RNA. These experiments indicate that mRNA corresponding to both the FB27/29 type, containing the second exon, and FB28 type, missing the second exon, are expressed in normal cells.

organization of the 3' Coding Exons Seguence comparison of the three fibroblast 5UBSTITUTE ~iHEET

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W091/00287 ~ 2 1 l3 PCT/US90/03729 clones de~onstrated that FB28 and FB29 contain a segment (exon 6) that is absent in FB27 .
Examination of the genomic DNA at a position corresponding to the position where the cDNAs diverge reveals a consensus splice donor (Sta~nezer et al., 1989, Mol. Cell. Biol . 9 , 4038-4045). The two cDNAs appear to derive from splicing events that either include or exclude exon 6. Downstream of this alternate exon the 3' portion of all three cDNAs are identical.
To investigate whether both types of c~NAs represent normal cellular mRNA, S1 analysis was carried out. Uniformly labeled single-stranded probes were generated from an 799-bp ~indIII
fragment (see probe D, Figure 1) from the 3' end of FB28 and a 497-bp ~indIII fragment from the 3' end ~f FB27 (probe C) subcloned in M13mpl8.
Single-stranded probes were prepared and were hybridized tc 20 ~g of total RNA prepared from whole chicken embryos of different ages. As seen in Figure 4E, hybridization of the RNA to probe D
(FB28) followed by Sl treatment produced a 799-bp fragment that would be expected if mR~A of FB28/29 type was present. Smaller fragments that would be generated by the FB28 probe hybridizing with mRNA
of the FB27 type were not detected. 51 digestion of the FB27 probe (probe C) produced a fragment of 497-bp and also two smaller fragments of 243 to 254 bp (see Figure 4D). It is likely that these smaller fragments derive from S1 cleavage of the FB27 probe at the site where the probe differs from mRNAs of the FB28/29 type. These data confirm the existence of the FB28/29 mRNA, but suggest that, if FB27 mRNA exists, it is likely to be present at a lower level than FB28/29.

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- . . . . . . . ... . . .

WOgl/00287 2 ~ v (~ pcT/usso/o3729 A schematic illustration of the differential splicing of three c-ski mRNAs as deduced from the nucleic acid sequence analysis of the cDNA clones is shown in Figure 3. Sl analysis of total ~NA
derived from chicken embryos has confirmed the existence of two classes of mRNAs, those that do, and those that do not, contain exon 2. Using a similar protocol, the existence of mRNA that contains exon 6 was confirmed. Utilizing this technigue, the existence of mRNA lacking exon 6 which would correspond to FB27 cDNA, could not be demonstrated; however, several lines of argument suggest that the FB27 c~NA is not a simple cloning artefact. The sequences absent from FB27 are bounded by apparent splice junctions (as judged by an examination of both the cDNA sequences and the available genomic DNA seguence). Although cDNAs are occasionally obtained that contain one or more introns, presumably because a partially processed mRNA was reverse transcribed, isolating cDNA
artefactually missing an exon is less likely on theoretical grounds, and seems to occur rarely, if at all, in the manufacture of a cDNA library. For these reasons, the interpretation that FB27 represents a real, if relatively rare, c-ski mRNA
is currently favored.

Comparison of c-ski with v-ski Transduction of c-oncs by retroviruses often results in truncation, deletion, or point mutation(s) in the c-onc (Bishop, J. M., 1983, Ann Rev. Biochem. 52, 301-354). Comparing c-ski and v-ski sequences shows that the v-ski gene is truncated at both 5' and 3' ends and represents only part of the c-ski coding region (see Figure 1 and 3~. v-s~i sequences begin at position 244 (the putative initiator ATG is at position 168) __ -: . ' ' ~
''' . . ' . :

.
.

WO91/00287 ~ r~ ~ ~3 PCT/US90/03729 - 21 _ and end at position 1541 ~see Flgure 3). The biological significance of the 5' and 3' truncations are unknown. There is only one base change in the v-ski relative to the c-ski, at position 1284 where v-ski has a 'C' and c-s~i has a 'T'. This base change alters the amino acid from Trp in c-ski to Arg in v-ski. Deletion analysis of v-ski implies that this amino acid change does not play a significant role in the transforming potential of v-ski. The biological activities of the cDNAs are being evaluated by expressing the coding regions from the cDNAs using replication-competent retroviral vectors (Hughes et al., 1987, J. Virol. 61, 3004-3012).
As shown in Figure 5, 18 of 20 bp are identical between c-ski and the pl9 region of gag in the parental ALV. This region of homology contains the 5' junction between viral sequences and v-ski. With such a long stretch of homology, it is impossible to assign the 5' recombinational joint precisely. No substantial homology can be seen precisely at the 3' ski/ALV joint. However, just downstream of the 3' junction, there is an ALV sequence that is closely homologous to a ~ segment of c-ski found just 3' of the v-ski/AVL
junction. It is possible to invoke this region of homology in the alignment of the nucleic acids involved in the recombination event.

Transduction mechanism The exact mechanism(s) by which retroviruses acquire cellular oncogenes remains uncertain. It has been suggested that as a first step, the viral DNA is integrated next to or within a cellular oncogene (Bishop, J. M., 1983, Ann Rev. Biochem.
52, 301-354). ~he retroviral and cellular seguences can then be fused by DNA deletion events ', , ' : , '' '-, ' .
, ' ' ' ' ~

wo 91/00287 2 /J 3 .~ ~ ~ f, Pcr/l,~sgo/03729 (Bishop, J.~. 1983, Ann. Rev. 8iochem. 52, 3 0 1-354; Czernilofsky et al., 1983, Nature 301, 736-738) or RNA read through (Herman et al., 1987, Science 236, 845-848; Nilsen et al., 1985, Cell 41, 1719-726); however, homology is not known to be invol~ed in either of these processes. In the cace of ski, a comparison of c-ski sequences with the pl9 coding region implies that some event in the generation of the ski viruses involved I0 homologous recombination. However, the homologous recombination event may be secondary. If the original event were nonhomologous and generated a viral genome that contained a direct repeat, it would be expected, as has been observed for Rous sarcoma virus, that sequences between the repeats could be lost by recombination during viral passage, presumably through copy choice during reverse transcription. It is possible, however, to propose models in which the oncogene is acquired by homologous recombination between the c-o~c and the replication competent viruses.
Short stretches of homologies have been observed at both ends of viral oncogenes (Banner et al., 1985, Mol. Cell. Biol. 5, 1400-1407; Van Beveren et al., 1983 Cell 32, 1241-1255) or only at the left hand or 5' recombination joint (Besmer et al., 1986, Nature (London) 320, 415-421; Roebroek et al., 1987, J. Virol. 61, 2009-20161. The present data does not allow the determination of whether the 5' homologous recombination event was prim~ry or secondary.

~ransgene and Generation of the Transgenic Mice The largest for~ of the isolated cDNAs, that `~ is FB29 which contains sequences that derive from all seven coding exons of c-ski, and judged by DNA
sequence, encodes a c-ski protein of 7S0 amino :
', .
- : : .

W09l/00~8~ li) rCT/U590/037~9 acids, was used to create the deri~ative ~FB29.
~FB29 has a frameshift mut:ation at position 147S
in the fifth coding exon ~one C in a run of five Cs was lost in the frameshift mutant), and is predicted to give rise to a protein of 448 amino acids of which the first 436 are identical to the first 436 amino acids of the FB29 form of c-ski (the last 12 amino acids are past the frameshift ~utation and thus differ from those of the FB29 foro of c-ski). ~FB29 used in the generation of a the transgene is shown schematically in Fiqure 6.
The construction of the ski portion of the transgene is already described tSutrave and Hughes, 1989, Mol. Cell. Biol. 9, 4046-4051;
Sutrave et al., 1990, Mol. Cell. Biol. 10, 3137-3144). Briefly, a truncated chicken c-ski cDNA
called ~FB29 had been previously cloned into the adaptor plasmid Clal2Nco. The ^FB2s seqment was released from the adaptor plasmid by Clal digestion and the 5' cverhangs filled in using the Xlenow fraqment of E. coli DNA polymerase I and all four dNTPs. This blunt-ended fragment was ligated to the pMEX neo vector which have been digested with Eco~1 restriction enzyme and blunt-ended with the Rlenow fragment. Clones wereselected that had inserts in the correct orientation and were digested with both PvuI and Nrul restriction endonucleases. These enzymes release a segment that contains the ~FB29 cDNA
flanked by an MSV LTR and the SV40 polyA siqnal (see Figure 6). This fragment was gel purified ~nd used to inject fertilized mouse eggs [Hogan et al., 1986, Manipulating the mouse embryo. A
Laboratory Manual. (Cold Spring Harbor, New York:
Cold Spring Harbor Laboratory)].
The ~FB29 clone was placed in the pMEX
expression plasmid in such an orientation that the - .
- .

, WO91/00287 ~ PcT~usso/o372s truncated c-ski cDNA between an MSV LT~ ant an SV40 polyadenylation site (see Figure 6). The plasmid was digested with PvuI and NruI to release the expression cassette. The expression cassette wa~ purified by gel electrophoresis and introduced into fertilized mouse eggs by microinjection.
Forty-Sour founder mice were obtained after two independent injections. The mice were identified by dot blot analysis of DNA isolated from tail cl~ps. Th~s analysis was confirmed by Southern transfer. Ihree of the 44 founder mice showed a distinct muscle phenotype (TG 8566, TG 8821, and TG 8S62). These three founders and a single mouse that contained unrearranged copy of the complete transgene but did not show any phenotype (TG 8542) were used to generate lines. Southern transfer analysis of DNA from TG 8566, TG 8821, TG 8562, and TG 8542 suggests that the site of integration of the transgene in each line is different and the copy number varies from approximately 5-35 copies per genome.
DNA positive mice from the three lines (TG
8566, TG 8821 and TG 8562) had a similar distinct appearance resulting from abnormal muscle growth.
Although the three lines of mice carry an oncogene, none of the lines appears to have an increased incidence of tumors. This result is not totally unexpected, since the v-ski virus is not t~origenic in chickens unless the birds are injected with infected cells (E. Stavnezer, 1988, ~n The oncogene Handbook, E. P. Reddy, A. Sk~lka, and T. Curran, eds. (Amsterdam: Elsevier Science Publishing Co.), pp. 393-401). The three strains of mice do not express high levels of the trans-gene except in skeletal muscle. This isconsistent with the interpretation that c-ski affects skeletal muscle cells directly and not as .
- .

~ Q ~

a secondary consequence of altered motor neuron function. The majority of the skeletal muscles are involved. Mice with this phenotype can be readily identified by looking for enlarged limb and jaw muscles (Figure 7). Since the phenotype was obtained with three separate founders, the most reasonable explanation is that the phenotype was caused by the chicken c-ski transgene.
Therefore, all four lines of mice were examined, the three with the muscular phenotype and the one line that did not have an observable phenotype, for the expression of the transgene.
The expression cassette was also introduced into fertilized pig eggs using standard procedures known to those in the art. Founder pigs show the expected muscle specific selection of expression of the gene and accordingly, it is expected that the pigs will have the same muscle phenotype as that seen in the founder mice.
DNA/RNA Analysis Total cellular DNA and RNA were isolated by standard procedures. For ~NA isolation, tissues were frozen in liquid nitrogen immediately following dissection and homogenized in RNAzol (Cinna Biotex) and processed according to the manufacturer's recommendation. For Northern transfer analysis, approximately 20 ~g of total RNA from different tissues was fractioned by electrophoresis on 1.5% agarose gels containing 2.2 M formaldehyde. The RNA was transferred to nitrocellulose membranes and probed either with a nick-translated chicken ski cDNA or a chicken ~-actin cDNA. The coding region of the ~-actin cDNA cross reacts with the messages for the other actions and can be used to validate the quantity and quality of RNA from most tissues.

;~3 ~

9 ~

Total RNA from spleen, lung, brain, kidney, liver, stomach, heart, and leg (skeletal) muscle was isolated as described and the results are shown in Figure 8. All three lines with the phenotype (TG 8566, TG 8821 and TG 8562) expressed a 2.5-kb chicken c-sk~ specific transcript at high levels in skeletal muscle; however, some lines of mice showed low levels of chicken c-ski RNA in other tissues. The TG 8562 line has RNA from the transgene in the heart, although at a lower level than in skeletal muscle. Histopathology of hearts from TG 8562 mice showed that there is no significant effect on this tissue. Line TG 8542, wh$ch does not show any phenotype, had a much lower level of RNA from the transgene in muscle than did the lines that showed the phenotype. The observation that expression was restricted to muscle was unexpected since the MSV LTR has been shown to express in a variety of tissues when linked to other genes (Xhillan et al., 1987, Genes Dev. 1, 1327-1~35~.
To determine whether the transcript was initiated at the proper site in tissues expressing the s~i transgene, RNase protection assays were carried out as described by Melton (Melton et al., 1984, Nucl. Acids Res. 12, 7035-7036). A 1.8-kb PVU1 to Bgll fragment was subcloned in Bluescript XS vector and used to generate radioactively labeled RNA from the T7 promoter. Approximately 10 ~g of total RNA was hybridized with 5 x 10' cpm of probe. The hybridizations were carried out overnight at 50C in 80% formamide and lx buffer (5x hybridization buffer is 0.2 M Pipes, pH 6.4, 2 M sodium chloride, 5 mM EDTA). After hybridization, the samples were diluted in ribonuclease digestion buffer (lO mM Tris Cl, pH
7.5, 0.~ M sodium chloride, 5 mM EDTA) and treated W091/00287 ~ ~ 9 ~ 2 ~ ~ PCT/USgOtO3729 - 2, -with RNase Tl at a concentration of 1 u/~l for 60 min at 30-C. The RNase digestions were stopped by adding lO ~l of 2Q% SDS and 4 ~l of Proteinase K
(stock lO mg/ml) and incubating at 37C for 15 min. The digested samples were extracted with phenol chloroform (l:l mixture) and ethanol precipitated with carrier tRNA. The pellet was rinsed once with 70% ethanol, dried and dissolved in formamide containing bromophenol blue and xylene cyanol dyes. The samples were denatured at lOO-C and separated on 6% polyacrylamide gels containing 7.5 M urea.
Uniformly labeled antisense RNA was generated by T7 RNA polymerase from a fragment that spans the MS~ L~R and c-ski (see Figure 9). Using RNA
from the three positive transgenic lines, a protected fragment of approximately 980 bases was seen, which is the expected size if the transcript i8 initiated at the authentic initiation site within the MSV LTR (Figure 9). This analysis also gives a more quantitative estimate o f the level of transgene RNA in the heart and skeletal muscle of both the phenotypically positive and the pheno-typically negative lines of mice. Figure g shows that the level of transgene RNA in the heart of TG
8821 is much lower (estimated at perhaps l/lO-l120) than the level found in the skeletal muscle.
In addition, this analysis shows that the phenotypically negative line, TG 8542, has a low but detectable level of transgene RNA in skeletal muscle. ~hese data suggest not only that the muscular phenotype is associated with the expression of the chicken c-ski transgene, but also that a minimum threshold level of c-ski RNA
must be reached to produce the muscular phenotype.
In fact, these data suggest that the minimal threshold level to see an effect of the transgene WO91/00287 ~ ~ 9 ~:~ 2 ~ ~ PCT/US90/03729 is high, probably several thousand-fold the levels of endogenous c-ski expression in the chicken tissues examined (Sutrave and Hughes, 1989, Mol.
Cell. Biol. 9, 4046-4051).

S Protein Analysis ~ he underlying assumption is not that the expression of the c-ski RNA gives rise directly t~
~he muscular phenotype, but rather that the phenotype results from the presence of the c-sk~
protein. Accordingly, the c-ski protein was looked at in Western transfer assays. Although rabbit antisera have been prepared that specifically recognize the 50-kd form of c-ski ISutrave et al., 1990, Mol. Cell. Biol. 10, 3137-lS 3144), these antisera do not work well in ~esterntransfer assays. Mouse monoclonal antibodies that recognize c-ski and that work well in Western transfer assays have been developed, however, the use oS these reagents presents a technical problem. These monoclonals were not available in sufficient quantity to permit direct labeling.
Indirect labeling procedures using, for example, labeled rabbit anti mouse detect not only the anti ski monoclonal but also the endogenous mouse heavy chain, which comigrates with the S0-kd form of c-6~i made from the transgene. To avoid this problem, extracts of muscle and control tissue ~liver) from normal controls and from the transgenic mice were prepared. The endogenous mouse antibodies were removed from these extracts by precipitation with rabbit anti mouse antibody as described below.
For detection of ski protein in tissues from the transgenic and control mice, 1-5 mg of tissue was homogenized in 1 ml of RIPA buffer, 20 mM Tris Cl pH 7.5, lS0 mM NaCl, o.s% SDS, 0.5% NP40, 0.5%

:

WO91/00287 2 ~ 3 PCT/~lS90/03729 sodiu~ deoxycholate, 1 mM EDTA, 1 mM PMSF, and 35 ~/ml of aproteinin. The homogenate was clarified by centrifugation at 10, oob rpm for lo min. Mouse IgG ~as removed from 100 ~1 of the supernatant by incubation with 10 ~1 of 1 mg/ml rabbit anti mouse IgG (in PBS) for 2 hr on ice. The complex was removed by adding 100 ~1 of 40% protein A
sepharose beads in RIPA buffer. ~he resulting supernatant was collected and 20 ~1 was fractionated on 10% SDS polyacrylamide gels. The proteins were transferred to nitrocellulose membranes overnight in buffer containing 0.125 M
Tris Cl, 0.092 M Glycine and 20% Methanol, pH 8.3.
The filters were blocked with 4% dry nonfat milk in TES buffer (0.5 M Tris Cl, pH 7.4 and .2 M
sodium chloride) for 2 hr at room temperature and incubated with a mixture of three anti-ski monoclonal antibodies at a dilution of 1:3000 for 2 hr at room temperature and were then washed 3x with TBS. Secondary incubations with rabbit anti ~ouse IgG were done for 2 hr at room temperature (1:2000 dilution from a 1 mg/ml stock). The filter was washed as described above and finally incubated with 5 ~Ci of "'I protein A (Amersham, æp. act. 30 mCi/mg) for 2 hr at room temperature.
The filter was washed 3x with TBS and exposed to XAR Xodak film at -70C for 6 days.
Only affected tissues (skeletal muscle) from the transgenic animals contain the 50-kd c-ski protein (Figure 10). These data also suggest that there may be differences in the level of the c-s~i protein in the muscles of the three positive lines; however, the complexities of the manipulations in this experiment make quantitative interpretation a questionable proposition.

~istology WO91/00287 ~ ~ ~ 9 I~J ~- ~3 PCT~US90/03729 For hlstology, selected muscles from the line TG 8566 were isolated so that they remained attached at their origin and insertion and they were then fixed in 2S formaldehyde, 2%
gluteraldehyde. Fixed muscle were transected precisely through the middle of the muscle belly and embedded in JB4 plastic (Polysciences, PA).
For immunocytochemistry, tissues were snap frozen in isopentane cooled in liquid nitrogen.
The procedure for immunochemical staining was as outlined in Narusawa et al. [(1987), J. Cell.
Biol. 104, 447-459].
The results showed that not only is expression of the transgene and its effect restricted to muscle, the expression and the effects appear, at least in the line ~G 8566, to be confined to certain muscles, and apparently to specific fiber types. However, the affected fibers are not all of the same type.
The myocardium was normal and there were no abnormalities of visceral smooth muscle in these animals. Figures lla and llb compare cross sections made precisely through the middle of the plantaris muscle from mature male controls and transgenic mice. Cross sectional area of the control is 2.7 ~' and that of the TG 8566 mouse is 9.4 ~', more than twice the control value. This massive growth is generalized. Comparable increases in cross section were found in almost all axial and appendicular muscles throughout male and female mice of line TG 8566. Only three muscles were found that appear to be normal: the tongue, the diaphragm, and the soleus; these are the same size in transgenic as in control muscles (see Figure 12). RNA was isolated from the diaphragm and soleus muscles of TG 8566 mice.
Northern transfer analysis shows that the level of : .
;
.
- : ~ .. ' .

WO91/0028/ 2~ 9 .(~ PCT/US90/03729 chicken c-s~l ~NA is much lower in these two phenotypically nor~al muscles than in the affected muscles from the same line (Figure 13).
The most obvious additional gross morphologic abnormality is that transgenic animals were almost totally devoid of fat whereas control animals contained substantial amounts of subcutaneous and intraperitoneal fat. For this reason, there is llttle difference in weights between control and TG 8566 mice. There are also skeletal abnormalities; the tibia of transgenic animals is normal in size but was bowed cranially, apparently as an adaptation to accommodate the more than two-fold increase in size of the anterior tibia and extensor digitorum muscles.
The muscles in control mice are made up of fibers with a range of cross sectional areas. The range of sizes is greatly extended in TG 8566 mice (Figures 11 and 12). Not all fibers are affected;
the hypertrophy is limited to a select population of fibers (Figure lld). Hypertrophy of these fibers apparently accounts for the increase in muscle mass in line TG 8566. No evidence was found that the numbers of fibers is significantly increased in individual muscles. For example, the population of fibers in the control plantaris is 912 + 111 (n-3) and in TG 8566 is 99~ + 87 (n-3).
Similarly, no diSference in the total number of fibers in the extensor digitorum longus muscle of TG 8566 and control mice was found.
To investigate which types of fibers are affected in the line TG 8566, three monoclonal antibodies were used that specifically recognize slow myosin heavy chains (MHC) (NOQ7 5 4D, Narusawa et al., 1987, J. Cell Biol. 104 , 447-459), all fast MHCs (2G3, Narusawa et al., 1987, J. Cell Biol. 104, 447-459) type II a fast MHC (sc .. , , ' WO gl/00287 ~ ~ 3 ~ 2 1 ~3 PCT/US90/03729 7 11, Schiafftno et al., 1989, J. Muscle Res. and Cell Mot~l. 10, 197-205) and type IIb fast MHC
(BF-F3, Schiaffino et al., 1989, J. Muscle Res.
and Cell Motil. 10, 197-205). Figure 14 shows t~unofluorescent staining of sections from the rhomboideus capitis muscle with these three antibodies. No evidence was found that slow fibers are enlarged (Figure 14a) and the total number of slow fibers in the rhomboideus capitis ~rom a TG 8566 mouse (120) approximates the number found in rhomboideus capitis from control mice (117). Type IIa fibers are also not affected (Figure 14b). Many of the type IIa fibers lie between hypertrophied fibers and are frequently distorted in shape as if compressed by the expansion of their neighbors (Figure 14b).
The small, fast IIa fibers and all of the hypertrophied fibers stain with the monoclonal antibody 2G3 (Figure 14c) indicating that all hypertrophied f~bers are fast. In the rhomboideus capitis, most, but not all, large fibers also stain with the monoclonal antibody BF-F3 that is specific for IIb MHC (Figure 14d). In the plantaris, there is more variation in reactivity and only 50% of hypertrophied fibers stain with BF-F3. ~hese results show that the hypertrophic modification of fibers in TG 8566 mice involves at least two types of fast fibers. One of these is type IIb. By exclusion, we suggest that the others are IIx fibers (Schi~ffino et al., 1989, J.
Muscle Res. and Cell Motil. 10, 197-205; Termin et al., 1989, Hlstochemistry 92, 453-457; Gorza, 1990, J. Histochem. Cytochem. 38, 257-265).
Variation in staining of bypertrophic fibers 3S with the actomyosin ATPase histochemical reaction after acid pre-i w ubation~ DPNH staining for mitochondrial enzyme activity and PAS staining all - . .
.

'. ~ ' :: . .
- ~

: : . : .

WO91/00287 2 a ~.~ 2 l ~ PCT/~S90/03729 support the conclusion that more than one fast fiber type is affected in this line of transgenic mice. These results also support the interpretation that both Ilb and IIx fibers are hy,pertrophied.
Occasional necrotic and reqenerating fibers were found in some, but not all, muscles of line TG 8566 mice. In the hind limb, these appeared to be most prevalent in the anterior tibial muscle;
they were never found in the rhomboideus capitis, a superficial muscle of the neck.

* * * * * *

All publications mentioned hereinabove are hereby incorporated in their entirety by reference.
While the foregoing invention has been described in some detail for purposes of clarity and understanding, it will be appreciated by one skilled in the art from a reading of this disclosure that various changes in form and detail can be made without departinq from the true scope of the invention and appended claims.

Claims (32)

1. A DNA segment encoding a chicken c-ski protein or a DNA fragment complementary to said DNA segment.
2. A DNA segment comprising exon 6 defined in Figure 1.
3. A DNA segment comprising exon 7 defined in Figure 1.
4. The DNA segment according to claim 3, further comprising at least four exons selected from the group consisting of: exon 1, exon 2, exon 3, exon 4, exon 5 and exon 6, defined in Figure 1.
5. A DNA construct comprising:
i) said DNA segment according to claim 1;
and ii) a vector.
6. The construct according to claim 5 wherein said vector is pMEX neo.
7. A DNA construct comprising:
i) a DNA segment encoding a truncated c-ski protein having the function of c-ski; and ii) a vector.
8. The DNA construct according to claim 7, wherein said DNA segment is .DELTA.FB29.
9. The DNA construct according to claim 7, wherein said vector is pMEX neo.
10. A host cell stably transformed with said construct according to claim 5, in a manner allowing expression of said protein encoded in said construct
11. A host cell stably transformed with said construct according to claim 7, in a manner allowing expression of said protein encoded in said construct.
12. The host cell according to claim 10 or claim 11 which is a mammalian cell.
13. A animal having increased muscle size, all of whose cells contain a DNA construct comprising a DNA segment encoding a ski protein and a vector, introduced into said animal, or an ancestor of said animal.
14. The animal according to claim 13 wherein said ski protein is a c-ski protein.
15. The animal according to claim 13 wherein said protein is a chicken c-ski protein.
16. The animal according to claim 13 wherein said vector is pMEX neo.
17. The animal according to claim 13 which is domestic livestock.
18. The animal according to claim 17 which is a pig.
19. The animal according to claim 13 which is a mammal.
20. The mammal according to claim 19 which is a mouse.
21. A animal having increased muscle size, all of whose cells contain a DNA construct comprising a DNA segment encoding a truncated ski protein having the function of ski and a vector, introduced into said animal, or an ancestor of said animal.
22. The animal according to claim 21 wherein said protein is a chicken c-ski protein.
23. The animal according to claim 21 wherein said DNA segment is .DELTA.FB29.
24. The animal according to claim 21 wherein said DNA segment encodes a v-ski protein.
25. The animal according to claim 21 wherein said vector is pMEX neo.
26. The animal according to claim 21 which is domestic livestock.
27. The animal according to claim 26 which is a pig.
28. The animal according to claim 21 which is a mammal.
29. The mammal according to claim 28 which is a mouse.
30. The use of a DNA construct comprising a DNA segment encoding a ski protein and a vector for stimulating muscle growth or preventing muscle degeneration, said DNA construct being delivered to muscle under conditions such that said protein of said construct is expressed and muscle growth induced.
31. The use of a DNA construct comprising a DNA segment encoding a ski protein and a vector for treating a muscle degenerative disease, said DNA construct being delivered to muscle under conditions such that said protein of said construct is expressed and treatment effected.
32. The use of the DNA construct of claim 31 wherein said muscle degenerative disease is muscular dystrophy or amyotrophic lateral sclerosis.
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