CA2090355A1 - Method of Gene Transfer Using Galactosylated Histones - Google Patents

Method of Gene Transfer Using Galactosylated Histones

Info

Publication number
CA2090355A1
CA2090355A1 CA 2090355 CA2090355A CA2090355A1 CA 2090355 A1 CA2090355 A1 CA 2090355A1 CA 2090355 CA2090355 CA 2090355 CA 2090355 A CA2090355 A CA 2090355A CA 2090355 A1 CA2090355 A1 CA 2090355A1
Authority
CA
Canada
Prior art keywords
protein
cells
genetic
histone
galactosylated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA 2090355
Other languages
French (fr)
Other versions
CA2090355C (en
Inventor
Jian Chen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CA 2090355 priority Critical patent/CA2090355C/en
Publication of CA2090355A1 publication Critical patent/CA2090355A1/en
Application granted granted Critical
Publication of CA2090355C publication Critical patent/CA2090355C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

The present invention relates to the field of introducing genetic material to cultured cells and into cells of animals in vivo. In particular, the invention relates to the use of receptor-targeted neoglycoprotein to carry genetic material into cells. The invention also uses nuclear-localization signals(Gerace et al. Annu. Rev. Cell Biol.
1988, 4:335-374), to facilitate the entry of genetic materials into cell nucleus for gene expression. The present invention has a wide variety of applications, for example, in treatment of genetic diseases, in study of cell biology, and in genetic interventions of physiological and pathological processes. The present invention is a effective method for introducing and expressing genes in hepatoma cells and hepatocytes in vivo. Its design has several novel features. First, this invention uses a simple coupling reagent (1-ethyl-3-(3-dimethylaminopropyl) carbodiimide) to chemically add galactose to a protein, histone, in a one step reaction. The galactosylated protein (for example, albumin, histone and any other protein) has high affinity for the ASGP receptor; Second, the invention uses a protein which is a natural DNA-binding protein; Three, this invention uses a protein which crosses the membranes of cell nucleus by a facilitated process in addition to simple diffusion. This feature contributes greatly to the high level expression of the introduced foreign genes; Last, the galactosylated histone 1 is the physical embodiment of all the features in this invention. This means this gene delivery system has only one component and is simple, reliable, and efficient from engineering-design point of view.
CA 2090355 1993-02-25 1993-02-25 Method of gene transfer using galactosylated histones Expired - Fee Related CA2090355C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA 2090355 CA2090355C (en) 1993-02-25 1993-02-25 Method of gene transfer using galactosylated histones

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CA 2090355 CA2090355C (en) 1993-02-25 1993-02-25 Method of gene transfer using galactosylated histones

Publications (2)

Publication Number Publication Date
CA2090355A1 true CA2090355A1 (en) 1994-08-26
CA2090355C CA2090355C (en) 1997-04-08

Family

ID=4151204

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2090355 Expired - Fee Related CA2090355C (en) 1993-02-25 1993-02-25 Method of gene transfer using galactosylated histones

Country Status (1)

Country Link
CA (1) CA2090355C (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001076638A2 (en) * 2000-04-12 2001-10-18 Implyx Ltd. Compositions for drug delivery

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001076638A2 (en) * 2000-04-12 2001-10-18 Implyx Ltd. Compositions for drug delivery
WO2001076638A3 (en) * 2000-04-12 2002-05-16 Implyx Ltd Compositions for drug delivery

Also Published As

Publication number Publication date
CA2090355C (en) 1997-04-08

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