CA2069629A1 - Process for isolating galactose oxidase - Google Patents

Process for isolating galactose oxidase

Info

Publication number
CA2069629A1
CA2069629A1 CA 2069629 CA2069629A CA2069629A1 CA 2069629 A1 CA2069629 A1 CA 2069629A1 CA 2069629 CA2069629 CA 2069629 CA 2069629 A CA2069629 A CA 2069629A CA 2069629 A1 CA2069629 A1 CA 2069629A1
Authority
CA
Canada
Prior art keywords
isolating
galactose oxidase
ultrafiltration
fungus
carboxy
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA 2069629
Other languages
French (fr)
Other versions
CA2069629C (en
Inventor
Laura Jane Crane
Adam Wieslaw Mazur
David Richard Nau
Bernard William Kluesener
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Procter and Gamble Co
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2069629A1 publication Critical patent/CA2069629A1/en
Application granted granted Critical
Publication of CA2069629C publication Critical patent/CA2069629C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A method of isolating and purifying galactose oxidase from a fungus media is disclosed. The supernatant liquid from the fermentation broth is subjected to ultrafiltration and then separated on a carboxy-sulfon cation exchange column using high pres-sure liquid chromatography. Copper ions must be present to insure enzyme activity.
CA 2069629 1989-12-20 1990-11-19 Process for isolating galactose oxidase Expired - Fee Related CA2069629C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US45372989A 1989-12-20 1989-12-20
US453,729 1989-12-20

Publications (2)

Publication Number Publication Date
CA2069629A1 true CA2069629A1 (en) 1991-06-21
CA2069629C CA2069629C (en) 1995-08-29

Family

ID=23801821

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2069629 Expired - Fee Related CA2069629C (en) 1989-12-20 1990-11-19 Process for isolating galactose oxidase

Country Status (5)

Country Link
EP (1) EP0506861A4 (en)
JP (1) JPH05503012A (en)
AU (1) AU7170691A (en)
CA (1) CA2069629C (en)
WO (1) WO1991009117A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW409035B (en) 1997-06-04 2000-10-21 Gist Brocades Bv Starch-based enzyme granulates

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS568686A (en) * 1979-07-05 1981-01-29 Kyowa Hakko Kogyo Co Ltd Preparation of galactose oxidase
US4661248A (en) * 1986-03-06 1987-04-28 J. T. Baker Chemical Company Sulfonic derivatives of acylated polyethyleneimine bonded phase silica products
US4721573A (en) * 1986-03-06 1988-01-26 J. T. Baker Chemical Company Use of sulfonic derivatives of acylated polyethyleneimine bonded phase silica products

Also Published As

Publication number Publication date
EP0506861A4 (en) 1993-02-17
EP0506861A1 (en) 1992-10-07
JPH05503012A (en) 1993-05-27
AU7170691A (en) 1991-07-18
WO1991009117A1 (en) 1991-06-27
CA2069629C (en) 1995-08-29

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Legal Events

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