CA2063176A1 - Process for the preparation of glutarylacylase in large quantities - Google Patents

Process for the preparation of glutarylacylase in large quantities

Info

Publication number
CA2063176A1
CA2063176A1 CA002063176A CA2063176A CA2063176A1 CA 2063176 A1 CA2063176 A1 CA 2063176A1 CA 002063176 A CA002063176 A CA 002063176A CA 2063176 A CA2063176 A CA 2063176A CA 2063176 A1 CA2063176 A1 CA 2063176A1
Authority
CA
Canada
Prior art keywords
glutarylacylase
preparation
large quantities
dsm
coli
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002063176A
Other languages
French (fr)
Other versions
CA2063176C (en
Inventor
Klaus-Peter Koller
Guenther J. Riess
Werner Aretz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sandoz AG
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2063176A1 publication Critical patent/CA2063176A1/en
Application granted granted Critical
Publication of CA2063176C publication Critical patent/CA2063176C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)

Abstract

Abstract of the disclosure A process for the preparation of glutarylacylase in large quantities The gene encoding glutarylacylase (GA) contained in the plasmid pCM 145 (DSM 6409) permits the expression of GA in E. coli in high yields.
CA002063176A 1991-03-18 1992-03-17 Process for the preparation of glutarylacylase in large quantities Expired - Fee Related CA2063176C (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE4108823 1991-03-18
DEP4108823.9 1991-03-18
DEP4136389.2 1991-11-05
DE4136389A DE4136389A1 (en) 1991-03-18 1991-11-05 PROCESS FOR THE PRODUCTION OF GLUTARYLACYLASE IN LARGE QUANTITIES

Publications (2)

Publication Number Publication Date
CA2063176A1 true CA2063176A1 (en) 1992-09-19
CA2063176C CA2063176C (en) 2004-06-01

Family

ID=25902010

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002063176A Expired - Fee Related CA2063176C (en) 1991-03-18 1992-03-17 Process for the preparation of glutarylacylase in large quantities

Country Status (14)

Country Link
EP (1) EP0504798B1 (en)
JP (1) JP3366664B2 (en)
KR (1) KR100211002B1 (en)
CN (1) CN1065292A (en)
AT (1) ATE145238T1 (en)
CA (1) CA2063176C (en)
CY (1) CY2115B1 (en)
CZ (1) CZ284405B6 (en)
DE (2) DE4136389A1 (en)
DK (1) DK0504798T3 (en)
ES (1) ES2096671T3 (en)
GR (1) GR3021610T3 (en)
IL (1) IL101258A0 (en)
SK (1) SK280242B6 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4337787A1 (en) * 1993-11-05 1995-05-11 Hoechst Ag Oxygen-dependent fermentation of microorganisms
IT1269180B (en) * 1994-01-14 1997-03-21 Mini Ricerca Scient Tecnolog PROCESS FOR THE PRODUCTION OF THE 7BETA ACID ENZYME - (4-CARBOXYBUTANAMIDE) -PHALOSPORANIC ACYLASE
DE4437420A1 (en) * 1994-10-19 1996-04-25 Hoechst Ag Improved, induction-free process for the genetic engineering of glutarylamidase
US5766871A (en) * 1996-11-27 1998-06-16 Food Industry Research And Development Institute Screening and characterization of glutaryl-7-aminocephalosporanic acid acylase
DE19915938A1 (en) 1999-04-09 2000-10-19 Aventis Pharma Gmbh Production of pancreatic procarboxypeptidase B, isoforms and muteins thereof and their use
DE19924632B4 (en) * 1999-05-28 2006-04-13 Sanofi-Aventis Deutschland Gmbh A method of delaying the deactivation of glutarylamidase during enzyme catalysis

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HU194307B (en) * 1983-09-16 1988-01-28 Richter Gedeon Vegyeszet Process for producing plasmidvectors of high copy number
DE3926076A1 (en) * 1989-04-21 1990-10-25 Boehringer Mannheim Gmbh RECOMBINANT DNA AND EXPRESSION VECTOR

Also Published As

Publication number Publication date
EP0504798A1 (en) 1992-09-23
DE4136389A1 (en) 1992-10-08
CS80492A3 (en) 1992-10-14
HK1006853A1 (en) 1999-03-19
GR3021610T3 (en) 1997-02-28
SK280242B6 (en) 1999-10-08
DK0504798T3 (en) 1997-04-07
EP0504798B1 (en) 1996-11-13
KR100211002B1 (en) 1999-07-15
JP3366664B2 (en) 2003-01-14
KR920018222A (en) 1992-10-21
CN1065292A (en) 1992-10-14
ES2096671T3 (en) 1997-03-16
IL101258A0 (en) 1992-11-15
DE4136389C2 (en) 1993-07-01
CZ284405B6 (en) 1998-11-11
CY2115B1 (en) 2002-04-26
ATE145238T1 (en) 1996-11-15
DE59207509D1 (en) 1996-12-19
JPH05207879A (en) 1993-08-20
CA2063176C (en) 2004-06-01

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Legal Events

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EEER Examination request
MKLA Lapsed