CA2050276A1 - Improved primer extension reactions - Google Patents

Improved primer extension reactions

Info

Publication number
CA2050276A1
CA2050276A1 CA002050276A CA2050276A CA2050276A1 CA 2050276 A1 CA2050276 A1 CA 2050276A1 CA 002050276 A CA002050276 A CA 002050276A CA 2050276 A CA2050276 A CA 2050276A CA 2050276 A1 CA2050276 A1 CA 2050276A1
Authority
CA
Canada
Prior art keywords
stranded region
primer extension
agent
extension reactions
extension
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002050276A
Other languages
French (fr)
Other versions
CA2050276C (en
Inventor
Stanley Tabor
Charles C. Richardson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Harvard University
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2050276A1 publication Critical patent/CA2050276A1/en
Application granted granted Critical
Publication of CA2050276C publication Critical patent/CA2050276C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)

Abstract

A kit or solution for use in extension of an oligonucleotide primer having a first.single-stranded region on a template mole-cule having a second single-stranded region homologous to the first single-stranded region; comprising a first agent able to cause extension of the first single-stranded region of the primer on the second single-stranded region of the template in a reaction mix-ture, and a second agent able to reduce the amount of pyrophosphate in the reaction mixture below the amount produced during the extension in the absence of the second agent.
CA002050276A 1989-04-12 1990-04-10 Improved primer extension reactions Expired - Lifetime CA2050276C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US33675189A 1989-04-12 1989-04-12
US336,751 1989-04-12
PCT/US1990/001938 WO1990012111A1 (en) 1989-04-12 1990-04-10 Improved primer extension reactions

Publications (2)

Publication Number Publication Date
CA2050276A1 true CA2050276A1 (en) 1990-10-13
CA2050276C CA2050276C (en) 2003-03-11

Family

ID=23317491

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002050276A Expired - Lifetime CA2050276C (en) 1989-04-12 1990-04-10 Improved primer extension reactions

Country Status (8)

Country Link
EP (1) EP0467953A4 (en)
JP (1) JP2997043B2 (en)
KR (1) KR920700294A (en)
AU (1) AU638246B2 (en)
CA (1) CA2050276C (en)
HU (1) HUT61054A (en)
LT (1) LTIP1519A (en)
WO (1) WO1990012111A1 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5001050A (en) * 1989-03-24 1991-03-19 Consejo Superior Investigaciones Cientificas PHφ29 DNA polymerase
US5198543A (en) * 1989-03-24 1993-03-30 Consejo Superior Investigaciones Cientificas PHI29 DNA polymerase
FR2674254B1 (en) * 1991-03-20 1995-10-06 Univ Reims Champagne Ardenne NON-RADIOACTIVE DETECTION OF THE PRESENCE OF A DETERMINED NUCLEIC ACID IN A BIOLOGICAL SAMPLE.
US5256555A (en) * 1991-12-20 1993-10-26 Ambion, Inc. Compositions and methods for increasing the yields of in vitro RNA transcription and other polynucleotide synthetic reactions
FI923911L (en) * 1992-09-01 1994-03-02 Vsevolod Kiselev DNA molecules in vitro synthesis
DE4336266A1 (en) * 1993-10-23 1995-04-27 Boehringer Mannheim Gmbh Stabilized liquid mixtures for labeling nucleic acids
CA2222744C (en) * 1995-05-31 2008-03-25 Amersham Life Science, Inc. Thermostable dna polymerases
US5665551A (en) * 1995-09-13 1997-09-09 Roche Molecular Systems, Inc. Purified nucleic acid encoding a thermostable pyrophosphatase
DE19612779A1 (en) * 1996-03-29 1997-10-02 Boehringer Mannheim Gmbh Method for the specific amplification of long nucleic acids by PCR
WO1998015655A1 (en) * 1996-10-07 1998-04-16 The Perkin-Elmer Corporation Primer extension reaction utilizing a cosubstrate-enzyme pair for consuming pyrophosphate
US6291164B1 (en) 1996-11-22 2001-09-18 Invitrogen Corporation Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate
GB0110501D0 (en) 2001-04-30 2001-06-20 Secr Defence Brit Amplification process
EP1704246A4 (en) * 2003-09-30 2007-11-28 Perkinelmer Las Inc Compositions and processes for genotyping single nucleotide polymorphisms
AU2014270410B2 (en) 2013-05-24 2020-07-16 Illumina Cambridge Limited Pyrophosphorolytic sequencing
CN117015616A (en) 2021-01-14 2023-11-07 合成Dna技术公司 Method for the production and quantification of beads labeled with unique molecular identifiers

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
CA1338457C (en) * 1986-08-22 1996-07-16 Henry A. Erlich Purified thermostable enzyme
US4971903A (en) * 1988-03-25 1990-11-20 Edward Hyman Pyrophosphate-based method and apparatus for sequencing nucleic acids

Also Published As

Publication number Publication date
EP0467953A4 (en) 1992-06-17
WO1990012111A1 (en) 1990-10-18
AU5438290A (en) 1990-11-05
JP2997043B2 (en) 2000-01-11
JPH04506002A (en) 1992-10-22
LTIP1519A (en) 1995-06-26
CA2050276C (en) 2003-03-11
AU638246B2 (en) 1993-06-24
HU903562D0 (en) 1992-03-30
EP0467953A1 (en) 1992-01-29
HUT61054A (en) 1992-11-30
KR920700294A (en) 1992-02-19

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Legal Events

Date Code Title Description
EEER Examination request
FZDC Correction of dead application (reinstatement)
MKEX Expiry