CA2045839A1 - Cloned n-methylhydantoinase - Google Patents

Cloned n-methylhydantoinase

Info

Publication number
CA2045839A1
CA2045839A1 CA002045839A CA2045839A CA2045839A1 CA 2045839 A1 CA2045839 A1 CA 2045839A1 CA 002045839 A CA002045839 A CA 002045839A CA 2045839 A CA2045839 A CA 2045839A CA 2045839 A1 CA2045839 A1 CA 2045839A1
Authority
CA
Canada
Prior art keywords
sequence
methylhydantoinase
activity
concerns
present
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002045839A
Other languages
French (fr)
Other versions
CA2045839C (en
Inventor
Gunther Schumacher
Helmut Burtscher
Hans Mollering
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Publication of CA2045839A1 publication Critical patent/CA2045839A1/en
Application granted granted Critical
Publication of CA2045839C publication Critical patent/CA2045839C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/86—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in cyclic amides, e.g. penicillinase (3.5.2)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention concerns a DNA which codes for a protein with N-methylhydantoinase activity and which has 1.) the nucleic acid sequence shown in SEQ ID NO: 1, 2.) a sequence corresponding to it within the scope of the degeneracy of the genetic code or 3.) a sequence which hybridizes with a sequence from (1) or/and (2) under stringent conditions.
Furthermore the invention also concerns a recombinant vector which contains a DNA according to the present invention, a cell which is transformed with a vector according to the present invention as well as a process for producing a recombinant protein with NMHase activity.
CA002045839A 1990-07-06 1991-06-27 Cloned n-methylhydantoinase Expired - Fee Related CA2045839C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DEP4021571.7 1990-07-06
DE4021571A DE4021571A1 (en) 1990-07-06 1990-07-06 N-METHYLHYDANTOINASE, CLONED

Publications (2)

Publication Number Publication Date
CA2045839A1 true CA2045839A1 (en) 1992-01-07
CA2045839C CA2045839C (en) 2001-12-18

Family

ID=6409795

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002045839A Expired - Fee Related CA2045839C (en) 1990-07-06 1991-06-27 Cloned n-methylhydantoinase

Country Status (10)

Country Link
US (2) US5213969A (en)
EP (1) EP0464832B1 (en)
JP (2) JP2546554B2 (en)
KR (1) KR950010819B1 (en)
AT (1) ATE173762T1 (en)
AU (1) AU628265B2 (en)
CA (1) CA2045839C (en)
DE (2) DE4021571A1 (en)
ES (1) ES2124694T3 (en)
ZA (1) ZA915211B (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9216851D0 (en) * 1992-08-07 1992-09-23 Univ Manitoba Dna sequences of rat probasin gene
KR100428725B1 (en) * 1996-03-30 2004-07-07 주식회사 현대시스콤 2 Phase Self Loopback Test Apparatus and Method of Digital Mobile Communication Switching Recording Device
US7960540B2 (en) * 1999-04-08 2011-06-14 Advanced Cancer Therapeutics, Llc Antiproliferative activity of G-rich oligonucleotides and method of using same to bind to nucleolin
US20080318890A1 (en) * 1999-04-08 2008-12-25 Antisoma Research Limited Antiproliferative activity of G-rich oligonucleotides and method of using same to bind to nucleolin
US8114850B2 (en) * 1999-04-08 2012-02-14 Advanced Cancer Therapeutics, Llc Antiproliferative activity of G-rich oligonucleotides and method of using same to bind to nucleolin
ES2296615T3 (en) * 1999-04-08 2008-05-01 Antisoma Research Limited ANTIPROLIFERATIVE ACTIVITY OF OLIGONUCLEOTIDOS RICH IN G AND PROCEDURE OF USE OF THE SAME FOR YOUR JOINT TO NUCLEOLINE.
US20080318889A1 (en) * 1999-04-08 2008-12-25 Antisoma Research Limited Antiproliferative activity of G-rich oligonucleotides and method of using same to bind to nucleolin
DE10130169A1 (en) * 2001-06-22 2003-01-09 Degussa Activated rec-D hydantoinases
JP2005523726A (en) * 2002-05-01 2005-08-11 ポリマー テクノロジー システムズ インコーポレーテッド Test strip for measuring creatinine concentration in body fluid and measuring method
US20090131351A1 (en) * 2007-11-16 2009-05-21 Antisoma Research Limited Methods, compositions, and kits for modulating tumor cell proliferation

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3406770A1 (en) * 1984-02-24 1985-08-29 Boehringer Mannheim Gmbh, 6800 Mannheim NUCLEOSIDE TRIPHOSPHATE-DEPENDENT 1-METHYL HYDANTOINASE AND THEIR USE
DE3535987A1 (en) * 1985-10-09 1987-04-23 Basf Ag METHOD FOR PRODUCING MEOPHILIC MICROORGANISMS THAT CONTAIN D-HYDANTOINASE ACTIVE AT HIGHER TEMPERATURE
DE3716957A1 (en) * 1987-05-20 1988-12-01 Boehringer Mannheim Gmbh EXPRESSION VECTOR FOR ADJUSTABLE EXPRESSION OF FOREIGN GENES IN PROKARYONTS
DE4000668A1 (en) * 1990-01-11 1991-07-18 Boehringer Mannheim Gmbh METHOD AND REAGENT FOR THE ENZYMATIC DETERMINATION OF CREATININE

Also Published As

Publication number Publication date
ZA915211B (en) 1992-04-29
DE59109072D1 (en) 1999-01-07
EP0464832B1 (en) 1998-11-25
AU628265B2 (en) 1992-09-10
JPH07313196A (en) 1995-12-05
EP0464832A3 (en) 1992-09-23
US5213969A (en) 1993-05-25
US5432070A (en) 1995-07-11
EP0464832A2 (en) 1992-01-08
AU7949991A (en) 1992-06-04
DE4021571A1 (en) 1992-01-16
ATE173762T1 (en) 1998-12-15
ES2124694T3 (en) 1999-02-16
KR950010819B1 (en) 1995-09-23
JP2546554B2 (en) 1996-10-23
JPH04229181A (en) 1992-08-18
KR920002786A (en) 1992-02-28
CA2045839C (en) 2001-12-18
JP2984906B2 (en) 1999-11-29

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