CA2043953A1 - Method for the isolation and expression of a gene which codes for streptokinase, nucleotide sequence obtained, recombinant dna and transformed microorganisms - Google Patents
Method for the isolation and expression of a gene which codes for streptokinase, nucleotide sequence obtained, recombinant dna and transformed microorganismsInfo
- Publication number
- CA2043953A1 CA2043953A1 CA 2043953 CA2043953A CA2043953A1 CA 2043953 A1 CA2043953 A1 CA 2043953A1 CA 2043953 CA2043953 CA 2043953 CA 2043953 A CA2043953 A CA 2043953A CA 2043953 A1 CA2043953 A1 CA 2043953A1
- Authority
- CA
- Canada
- Prior art keywords
- streptokinase
- codes
- expression
- gene
- isolation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
The present invention relates to the field of biotechnology and genetic engineering and in particular a novel nucleotide sequence which codes for a streptokinase, as well as the recombinant DNA obtained therefrom which is used for the transformation of various host organisms. The technical object which is pursued with the proposed solution is to produce the protein in commercially useful quantities, by the recombinant method, with the possibility of alternatively using different hosts to obtain a high-quality streptokinase capable of being used as a thrombolytic agent in the treatment of different disorders. The present invention is based on the isolation of a new gene which codes for streptokinase from Streptococcus equisimilis of type C (strain ATCC-9542) and the cloning and expression thereof in prokaryotic (E. coli) and eukaryotic (Pichia pastoris) hosts, for which is includes the vehicles of expression which contain the genetic sequences of said gene, as well as the microorganisms transformed with these vectors capable of producing streptokinase. The protein obtained thereby can be used in clinical medicine as a therapeutic agent, in the treatment of disorders such as thromboembolic obstructions including coronary thrombosis.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
HU1770/91A HU216073B (en) | 1991-05-27 | 1991-05-27 | Process for producing streptokinase |
CA 2043953 CA2043953C (en) | 1991-06-05 | 1991-06-05 | Method for the isolation and expression of a gene which codes for streptokinase, nucleotide sequence obtained, recombinant dna and transformed microorganisms |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA 2043953 CA2043953C (en) | 1991-06-05 | 1991-06-05 | Method for the isolation and expression of a gene which codes for streptokinase, nucleotide sequence obtained, recombinant dna and transformed microorganisms |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2043953A1 true CA2043953A1 (en) | 1992-12-06 |
CA2043953C CA2043953C (en) | 2001-06-12 |
Family
ID=4147743
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA 2043953 Expired - Fee Related CA2043953C (en) | 1991-05-27 | 1991-06-05 | Method for the isolation and expression of a gene which codes for streptokinase, nucleotide sequence obtained, recombinant dna and transformed microorganisms |
Country Status (1)
Country | Link |
---|---|
CA (1) | CA2043953C (en) |
-
1991
- 1991-06-05 CA CA 2043953 patent/CA2043953C/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
CA2043953C (en) | 2001-06-12 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKLA | Lapsed |