CA2043953A1 - Method for the isolation and expression of a gene which codes for streptokinase, nucleotide sequence obtained, recombinant dna and transformed microorganisms - Google Patents

Method for the isolation and expression of a gene which codes for streptokinase, nucleotide sequence obtained, recombinant dna and transformed microorganisms

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Publication number
CA2043953A1
CA2043953A1 CA 2043953 CA2043953A CA2043953A1 CA 2043953 A1 CA2043953 A1 CA 2043953A1 CA 2043953 CA2043953 CA 2043953 CA 2043953 A CA2043953 A CA 2043953A CA 2043953 A1 CA2043953 A1 CA 2043953A1
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CA
Canada
Prior art keywords
streptokinase
codes
expression
gene
isolation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA 2043953
Other languages
French (fr)
Other versions
CA2043953C (en
Inventor
Mario P. E. Garcia
Roger R. Chaplen
Aimee P. Hidalgo
Ricardo S. Doce
Luciano F. H. Marrero
Pedro R. Collazo
Anaisel C. Ramirez
Emilio A. M. Munoz
Walfrido B. Martinez
Magalys C. Somavilla
Alicia P. Fernandez
Jose De J. De La Fuente Garcia
Luis S. H. Martinez
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centro de Ingenieria Genetica y Biotecnologia CIGB
Original Assignee
Mario P. E. Garcia
Roger R. Chaplen
Aimee P. Hidalgo
Ricardo S. Doce
Luciano F. H. Marrero
Pedro R. Collazo
Anaisel C. Ramirez
Emilio A. M. Munoz
Walfrido B. Martinez
Magalys C. Somavilla
Alicia P. Fernandez
Jose De J. De La Fuente Garcia
Luis S. H. Martinez
Centro De Ingenieria Genetica Y Biotecnologia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to HU1770/91A priority Critical patent/HU216073B/en
Application filed by Mario P. E. Garcia, Roger R. Chaplen, Aimee P. Hidalgo, Ricardo S. Doce, Luciano F. H. Marrero, Pedro R. Collazo, Anaisel C. Ramirez, Emilio A. M. Munoz, Walfrido B. Martinez, Magalys C. Somavilla, Alicia P. Fernandez, Jose De J. De La Fuente Garcia, Luis S. H. Martinez, Centro De Ingenieria Genetica Y Biotecnologia filed Critical Mario P. E. Garcia
Priority to CA 2043953 priority patent/CA2043953C/en
Publication of CA2043953A1 publication Critical patent/CA2043953A1/en
Application granted granted Critical
Publication of CA2043953C publication Critical patent/CA2043953C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The present invention relates to the field of biotechnology and genetic engineering and in particular a novel nucleotide sequence which codes for a streptokinase, as well as the recombinant DNA obtained therefrom which is used for the transformation of various host organisms. The technical object which is pursued with the proposed solution is to produce the protein in commercially useful quantities, by the recombinant method, with the possibility of alternatively using different hosts to obtain a high-quality streptokinase capable of being used as a thrombolytic agent in the treatment of different disorders. The present invention is based on the isolation of a new gene which codes for streptokinase from Streptococcus equisimilis of type C (strain ATCC-9542) and the cloning and expression thereof in prokaryotic (E. coli) and eukaryotic (Pichia pastoris) hosts, for which is includes the vehicles of expression which contain the genetic sequences of said gene, as well as the microorganisms transformed with these vectors capable of producing streptokinase. The protein obtained thereby can be used in clinical medicine as a therapeutic agent, in the treatment of disorders such as thromboembolic obstructions including coronary thrombosis.
CA 2043953 1991-05-27 1991-06-05 Method for the isolation and expression of a gene which codes for streptokinase, nucleotide sequence obtained, recombinant dna and transformed microorganisms Expired - Fee Related CA2043953C (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
HU1770/91A HU216073B (en) 1991-05-27 1991-05-27 Process for producing streptokinase
CA 2043953 CA2043953C (en) 1991-06-05 1991-06-05 Method for the isolation and expression of a gene which codes for streptokinase, nucleotide sequence obtained, recombinant dna and transformed microorganisms

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CA 2043953 CA2043953C (en) 1991-06-05 1991-06-05 Method for the isolation and expression of a gene which codes for streptokinase, nucleotide sequence obtained, recombinant dna and transformed microorganisms

Publications (2)

Publication Number Publication Date
CA2043953A1 true CA2043953A1 (en) 1992-12-06
CA2043953C CA2043953C (en) 2001-06-12

Family

ID=4147743

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2043953 Expired - Fee Related CA2043953C (en) 1991-05-27 1991-06-05 Method for the isolation and expression of a gene which codes for streptokinase, nucleotide sequence obtained, recombinant dna and transformed microorganisms

Country Status (1)

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CA (1) CA2043953C (en)

Also Published As

Publication number Publication date
CA2043953C (en) 2001-06-12

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