CA2040913A1 - Method and element for measuring analytes in biological fluids using immobilized binder-analyte labeled complex - Google Patents

Method and element for measuring analytes in biological fluids using immobilized binder-analyte labeled complex

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Publication number
CA2040913A1
CA2040913A1 CA 2040913 CA2040913A CA2040913A1 CA 2040913 A1 CA2040913 A1 CA 2040913A1 CA 2040913 CA2040913 CA 2040913 CA 2040913 A CA2040913 A CA 2040913A CA 2040913 A1 CA2040913 A1 CA 2040913A1
Authority
CA
Canada
Prior art keywords
analyte
binder
biological fluids
immobilized
measuring analytes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA 2040913
Other languages
French (fr)
Other versions
CA2040913C (en
Inventor
A. Said El Shami
Christopher W. Hand
Susan A. Miller
Robert A. Moore
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Siemens Medical Solutions Diagnostics Inc Canada
Original Assignee
Diagnostic Products Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Diagnostic Products Corp filed Critical Diagnostic Products Corp
Priority to CA 2040913 priority Critical patent/CA2040913C/en
Publication of CA2040913A1 publication Critical patent/CA2040913A1/en
Application granted granted Critical
Publication of CA2040913C publication Critical patent/CA2040913C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Abstract

The method of measuring analytes in biological fluids is disclosed wherein a specific binder to a given analyte is covalently immobilized onto a solid support to which a labeled analyte is pre-reacted and stabilized to form a binder-labeled analyte complex. A sample is contacted with said immobilized complex wherein an analyte in the sample, if present, competes with the labeled analyte bound to the immobilized binder for binding sites on said binder thus displacing a given amount of the labeled analyte which is directly proportional to the amount of analyte present in the sample. The affinity of the labeled analyte to the analyte's specific binder is lower than the affinity of the unlabeled analyte to the same binder.
CA 2040913 1991-04-22 1991-04-22 Method and element for measuring analytes in biological fluids using immobilized binder-analyte labeled complex Expired - Fee Related CA2040913C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA 2040913 CA2040913C (en) 1991-04-22 1991-04-22 Method and element for measuring analytes in biological fluids using immobilized binder-analyte labeled complex

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CA 2040913 CA2040913C (en) 1991-04-22 1991-04-22 Method and element for measuring analytes in biological fluids using immobilized binder-analyte labeled complex

Publications (2)

Publication Number Publication Date
CA2040913A1 true CA2040913A1 (en) 1992-10-23
CA2040913C CA2040913C (en) 1999-10-19

Family

ID=4147450

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2040913 Expired - Fee Related CA2040913C (en) 1991-04-22 1991-04-22 Method and element for measuring analytes in biological fluids using immobilized binder-analyte labeled complex

Country Status (1)

Country Link
CA (1) CA2040913C (en)

Also Published As

Publication number Publication date
CA2040913C (en) 1999-10-19

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