CA2023363A1 - Use of amide complex compounds - Google Patents
Use of amide complex compoundsInfo
- Publication number
- CA2023363A1 CA2023363A1 CA 2023363 CA2023363A CA2023363A1 CA 2023363 A1 CA2023363 A1 CA 2023363A1 CA 2023363 CA2023363 CA 2023363 CA 2023363 A CA2023363 A CA 2023363A CA 2023363 A1 CA2023363 A1 CA 2023363A1
- Authority
- CA
- Canada
- Prior art keywords
- aryl
- alkyl
- substituted
- carbamoyl
- atoms
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 150000001875 compounds Chemical class 0.000 title claims abstract description 44
- 150000001408 amides Chemical class 0.000 title description 8
- 125000004432 carbon atom Chemical group C* 0.000 claims abstract description 28
- 150000003839 salts Chemical class 0.000 claims abstract description 22
- 125000004169 (C1-C6) alkyl group Chemical group 0.000 claims abstract description 20
- 229910021645 metal ion Inorganic materials 0.000 claims abstract description 16
- 125000004191 (C1-C6) alkoxy group Chemical group 0.000 claims abstract description 14
- 150000007530 organic bases Chemical class 0.000 claims abstract description 14
- 150000007529 inorganic bases Chemical class 0.000 claims abstract description 13
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 13
- 229920006395 saturated elastomer Polymers 0.000 claims abstract description 13
- 125000003917 carbamoyl group Chemical group [H]N([H])C(*)=O 0.000 claims abstract description 12
- 125000004430 oxygen atom Chemical group O* 0.000 claims abstract description 8
- 125000004890 (C1-C6) alkylamino group Chemical group 0.000 claims abstract description 7
- 125000001797 benzyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])* 0.000 claims abstract description 7
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 claims abstract description 7
- 125000004434 sulfur atom Chemical group 0.000 claims abstract description 7
- SEZWJRWTCIPRSV-UHFFFAOYSA-N [N]C(N)=O Chemical compound [N]C(N)=O SEZWJRWTCIPRSV-UHFFFAOYSA-N 0.000 claims abstract description 6
- 125000000383 tetramethylene group Chemical group [H]C([H])([*:1])C([H])([H])C([H])([H])C([H])([H])[*:2] 0.000 claims abstract description 6
- 125000003258 trimethylene group Chemical group [H]C([H])([*:2])C([H])([H])C([H])([H])[*:1] 0.000 claims abstract description 6
- 210000000056 organ Anatomy 0.000 claims abstract description 5
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 5
- 208000001647 Renal Insufficiency Diseases 0.000 claims abstract description 4
- 201000006370 kidney failure Diseases 0.000 claims abstract description 4
- 238000000034 method Methods 0.000 claims description 43
- -1 hydroxy, carbamoyl Chemical group 0.000 claims description 29
- 229910052688 Gadolinium Inorganic materials 0.000 claims description 28
- UIWYJDYFSGRHKR-UHFFFAOYSA-N gadolinium atom Chemical compound [Gd] UIWYJDYFSGRHKR-UHFFFAOYSA-N 0.000 claims description 28
- 239000002253 acid Substances 0.000 claims description 26
- QPCDCPDFJACHGM-UHFFFAOYSA-N N,N-bis{2-[bis(carboxymethyl)amino]ethyl}glycine Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(=O)O)CCN(CC(O)=O)CC(O)=O QPCDCPDFJACHGM-UHFFFAOYSA-N 0.000 claims description 21
- 125000000041 C6-C10 aryl group Chemical group 0.000 claims description 19
- 239000003795 chemical substances by application Substances 0.000 claims description 15
- PAYRUJLWNCNPSJ-UHFFFAOYSA-N Aniline Chemical compound NC1=CC=CC=C1 PAYRUJLWNCNPSJ-UHFFFAOYSA-N 0.000 claims description 12
- WGQKYBSKWIADBV-UHFFFAOYSA-N benzylamine Chemical compound NCC1=CC=CC=C1 WGQKYBSKWIADBV-UHFFFAOYSA-N 0.000 claims description 11
- 229910052739 hydrogen Inorganic materials 0.000 claims description 10
- 239000002872 contrast media Substances 0.000 claims description 9
- 238000003384 imaging method Methods 0.000 claims description 6
- 230000006872 improvement Effects 0.000 claims description 6
- 125000004429 atom Chemical group 0.000 claims description 5
- 239000008139 complexing agent Substances 0.000 claims description 5
- OAKJQQAXSVQMHS-UHFFFAOYSA-N Hydrazine Chemical compound NN OAKJQQAXSVQMHS-UHFFFAOYSA-N 0.000 claims description 4
- 208000018522 Gastrointestinal disease Diseases 0.000 claims description 3
- IOIFRTZBJMZZFO-UHFFFAOYSA-N dysprosium(3+) Chemical compound [Dy+3] IOIFRTZBJMZZFO-UHFFFAOYSA-N 0.000 claims description 3
- 210000000232 gallbladder Anatomy 0.000 claims description 3
- YBRBMKDOPFTVDT-UHFFFAOYSA-N tert-butylamine Chemical compound CC(C)(C)N YBRBMKDOPFTVDT-UHFFFAOYSA-N 0.000 claims description 3
- XFGDUFPPWVQZMH-UHFFFAOYSA-N 2-[2-[bis(carboxymethyl)amino]ethyl-[2-[carboxymethyl-[2-(hexadecylamino)-2-oxoethyl]amino]ethyl]amino]acetic acid Chemical compound CCCCCCCCCCCCCCCCNC(=O)CN(CC(O)=O)CCN(CC(O)=O)CCN(CC(O)=O)CC(O)=O XFGDUFPPWVQZMH-UHFFFAOYSA-N 0.000 claims description 2
- 239000003963 antioxidant agent Substances 0.000 claims description 2
- 210000000013 bile duct Anatomy 0.000 claims description 2
- 239000004305 biphenyl Substances 0.000 claims description 2
- 239000000872 buffer Substances 0.000 claims description 2
- 206010017758 gastric cancer Diseases 0.000 claims description 2
- 208000010749 gastric carcinoma Diseases 0.000 claims description 2
- 210000004185 liver Anatomy 0.000 claims description 2
- 210000005227 renal system Anatomy 0.000 claims description 2
- 201000000498 stomach carcinoma Diseases 0.000 claims description 2
- 125000006719 (C6-C10) aryl (C1-C6) alkyl group Chemical group 0.000 claims 17
- 150000001338 aliphatic hydrocarbons Chemical class 0.000 claims 16
- 125000004209 (C1-C8) alkyl group Chemical group 0.000 claims 4
- 125000004433 nitrogen atom Chemical group N* 0.000 claims 3
- 208000007107 Stomach Ulcer Diseases 0.000 claims 1
- 230000003078 antioxidant effect Effects 0.000 claims 1
- 230000037396 body weight Effects 0.000 claims 1
- 208000010643 digestive system disease Diseases 0.000 claims 1
- 239000003937 drug carrier Substances 0.000 claims 1
- 239000003792 electrolyte Substances 0.000 claims 1
- 201000005917 gastric ulcer Diseases 0.000 claims 1
- 208000018685 gastrointestinal system disease Diseases 0.000 claims 1
- 125000004435 hydrogen atom Chemical group [H]* 0.000 abstract description 21
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 14
- 125000000753 cycloalkyl group Chemical group 0.000 abstract description 8
- 125000001424 substituent group Chemical group 0.000 abstract description 8
- 125000000217 alkyl group Chemical group 0.000 abstract description 5
- 125000002887 hydroxy group Chemical group [H]O* 0.000 abstract description 4
- 125000003710 aryl alkyl group Chemical group 0.000 abstract description 3
- 125000003118 aryl group Chemical group 0.000 abstract description 3
- 125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 abstract description 3
- 229910052717 sulfur Inorganic materials 0.000 abstract description 3
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 abstract description 2
- 239000001301 oxygen Substances 0.000 abstract description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 66
- 239000000243 solution Substances 0.000 description 49
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 46
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 30
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 24
- 239000000843 powder Substances 0.000 description 22
- 238000004458 analytical method Methods 0.000 description 19
- 239000000203 mixture Substances 0.000 description 19
- ZMANZCXQSJIPKH-UHFFFAOYSA-N Triethylamine Chemical compound CCN(CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-N 0.000 description 18
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 15
- 239000002904 solvent Substances 0.000 description 15
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 12
- 238000012937 correction Methods 0.000 description 12
- MBBZMMPHUWSWHV-BDVNFPICSA-N N-methylglucamine Chemical compound CNC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO MBBZMMPHUWSWHV-BDVNFPICSA-N 0.000 description 10
- 238000006243 chemical reaction Methods 0.000 description 10
- 150000002500 ions Chemical class 0.000 description 10
- WFDIJRYMOXRFFG-UHFFFAOYSA-N Acetic anhydride Chemical compound CC(=O)OC(C)=O WFDIJRYMOXRFFG-UHFFFAOYSA-N 0.000 description 9
- RWRDLPDLKQPQOW-UHFFFAOYSA-N Pyrrolidine Chemical compound C1CCNC1 RWRDLPDLKQPQOW-UHFFFAOYSA-N 0.000 description 9
- 230000002378 acidificating effect Effects 0.000 description 9
- 150000001768 cations Chemical class 0.000 description 8
- 229920001429 chelating resin Polymers 0.000 description 8
- CMIHHWBVHJVIGI-UHFFFAOYSA-N gadolinium(III) oxide Inorganic materials [O-2].[O-2].[O-2].[Gd+3].[Gd+3] CMIHHWBVHJVIGI-UHFFFAOYSA-N 0.000 description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 7
- YNAVUWVOSKDBBP-UHFFFAOYSA-N Morpholine Chemical group C1COCCN1 YNAVUWVOSKDBBP-UHFFFAOYSA-N 0.000 description 7
- 238000004108 freeze drying Methods 0.000 description 7
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 6
- 125000002057 carboxymethyl group Chemical group [H]OC(=O)C([H])([H])[*] 0.000 description 6
- 229940075613 gadolinium oxide Drugs 0.000 description 6
- 229910001938 gadolinium oxide Inorganic materials 0.000 description 6
- 125000000623 heterocyclic group Chemical group 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 6
- 239000012528 membrane Substances 0.000 description 6
- 230000007935 neutral effect Effects 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 239000000725 suspension Substances 0.000 description 6
- 239000007983 Tris buffer Substances 0.000 description 5
- 229960000583 acetic acid Drugs 0.000 description 5
- 238000007792 addition Methods 0.000 description 5
- 150000001413 amino acids Chemical class 0.000 description 5
- 125000004494 ethyl ester group Chemical group 0.000 description 5
- HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 description 4
- RQFNYUNDFYFHBI-UHFFFAOYSA-N 2-[2-[carboxymethyl-(2-ethoxy-2-oxoethyl)amino]ethyl-[2-(2,6-dioxomorpholin-4-yl)ethyl]amino]acetic acid Chemical compound CCOC(=O)CN(CC(O)=O)CCN(CC(O)=O)CCN1CC(=O)OC(=O)C1 RQFNYUNDFYFHBI-UHFFFAOYSA-N 0.000 description 4
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 4
- NQRYJNQNLNOLGT-UHFFFAOYSA-N Piperidine Chemical compound C1CCNCC1 NQRYJNQNLNOLGT-UHFFFAOYSA-N 0.000 description 4
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 4
- 230000000536 complexating effect Effects 0.000 description 4
- 229940039231 contrast media Drugs 0.000 description 4
- 230000008030 elimination Effects 0.000 description 4
- 238000003379 elimination reaction Methods 0.000 description 4
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 4
- 239000000706 filtrate Substances 0.000 description 4
- LGMLJQFQKXPRGA-VPVMAENOSA-K gadopentetate dimeglumine Chemical compound [Gd+3].CNC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO.CNC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO.OC(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O LGMLJQFQKXPRGA-VPVMAENOSA-K 0.000 description 4
- 229910052751 metal Inorganic materials 0.000 description 4
- 239000002184 metal Substances 0.000 description 4
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 125000001436 propyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])[H] 0.000 description 4
- 239000000741 silica gel Substances 0.000 description 4
- 229910002027 silica gel Inorganic materials 0.000 description 4
- 239000011734 sodium Substances 0.000 description 4
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- FXHOOIRPVKKKFG-UHFFFAOYSA-N N,N-Dimethylacetamide Chemical compound CN(C)C(C)=O FXHOOIRPVKKKFG-UHFFFAOYSA-N 0.000 description 3
- 206010028980 Neoplasm Diseases 0.000 description 3
- 238000013019 agitation Methods 0.000 description 3
- 125000003368 amide group Chemical group 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 238000009835 boiling Methods 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- UAOMVDZJSHZZME-UHFFFAOYSA-N diisopropylamine Chemical compound CC(C)NC(C)C UAOMVDZJSHZZME-UHFFFAOYSA-N 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 150000002148 esters Chemical class 0.000 description 3
- 230000029142 excretion Effects 0.000 description 3
- 239000012362 glacial acetic acid Substances 0.000 description 3
- 125000001449 isopropyl group Chemical group [H]C([H])([H])C([H])(*)C([H])([H])[H] 0.000 description 3
- 238000006386 neutralization reaction Methods 0.000 description 3
- 238000007911 parenteral administration Methods 0.000 description 3
- 159000000000 sodium salts Chemical class 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- IDPURXSQCKYKIJ-UHFFFAOYSA-N 1-(4-methoxyphenyl)methanamine Chemical compound COC1=CC=C(CN)C=C1 IDPURXSQCKYKIJ-UHFFFAOYSA-N 0.000 description 2
- VRJHQPZVIGNGMX-UHFFFAOYSA-N 4-piperidinone Chemical compound O=C1CCNCC1 VRJHQPZVIGNGMX-UHFFFAOYSA-N 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 239000004475 Arginine Substances 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- ZAFNJMIOTHYJRJ-UHFFFAOYSA-N Diisopropyl ether Chemical compound CC(C)OC(C)C ZAFNJMIOTHYJRJ-UHFFFAOYSA-N 0.000 description 2
- XTHFKEDIFFGKHM-UHFFFAOYSA-N Dimethoxyethane Chemical compound COCCOC XTHFKEDIFFGKHM-UHFFFAOYSA-N 0.000 description 2
- ROSDSFDQCJNGOL-UHFFFAOYSA-N Dimethylamine Chemical compound CNC ROSDSFDQCJNGOL-UHFFFAOYSA-N 0.000 description 2
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- 206010061218 Inflammation Diseases 0.000 description 2
- AHLPHDHHMVZTML-BYPYZUCNSA-N L-Ornithine Chemical compound NCCC[C@H](N)C(O)=O AHLPHDHHMVZTML-BYPYZUCNSA-N 0.000 description 2
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 2
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 2
- 239000004472 Lysine Substances 0.000 description 2
- BZLVMXJERCGZMT-UHFFFAOYSA-N Methyl tert-butyl ether Chemical compound COC(C)(C)C BZLVMXJERCGZMT-UHFFFAOYSA-N 0.000 description 2
- AHLPHDHHMVZTML-UHFFFAOYSA-N Orn-delta-NH2 Natural products NCCCC(N)C(O)=O AHLPHDHHMVZTML-UHFFFAOYSA-N 0.000 description 2
- UTJLXEIPEHZYQJ-UHFFFAOYSA-N Ornithine Natural products OC(=O)C(C)CCCN UTJLXEIPEHZYQJ-UHFFFAOYSA-N 0.000 description 2
- OFBQJSOFQDEBGM-UHFFFAOYSA-N Pentane Chemical compound CCCCC OFBQJSOFQDEBGM-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- WYURNTSHIVDZCO-UHFFFAOYSA-N Tetrahydrofuran Chemical compound C1CCOC1 WYURNTSHIVDZCO-UHFFFAOYSA-N 0.000 description 2
- 150000008065 acid anhydrides Chemical class 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 150000001412 amines Chemical class 0.000 description 2
- 150000008064 anhydrides Chemical class 0.000 description 2
- 238000005349 anion exchange Methods 0.000 description 2
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 125000000484 butyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 2
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 238000002405 diagnostic procedure Methods 0.000 description 2
- JQVDAXLFBXTEQA-UHFFFAOYSA-N dibutylamine Chemical compound CCCCNCCCC JQVDAXLFBXTEQA-UHFFFAOYSA-N 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- NLQFUUYNQFMIJW-UHFFFAOYSA-N dysprosium(iii) oxide Chemical compound O=[Dy]O[Dy]=O NLQFUUYNQFMIJW-UHFFFAOYSA-N 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 238000001704 evaporation Methods 0.000 description 2
- 230000008020 evaporation Effects 0.000 description 2
- 210000001035 gastrointestinal tract Anatomy 0.000 description 2
- 229930195733 hydrocarbon Natural products 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- 230000004054 inflammatory process Effects 0.000 description 2
- 238000010253 intravenous injection Methods 0.000 description 2
- 125000000959 isobutyl group Chemical group [H]C([H])([H])C([H])(C([H])([H])[H])C([H])([H])* 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 239000003446 ligand Substances 0.000 description 2
- 239000007791 liquid phase Substances 0.000 description 2
- GVWISOJSERXQBM-UHFFFAOYSA-N n-methylpropan-1-amine Chemical compound CCCNC GVWISOJSERXQBM-UHFFFAOYSA-N 0.000 description 2
- 229960003104 ornithine Drugs 0.000 description 2
- 125000001147 pentyl group Chemical group C(CCCC)* 0.000 description 2
- 239000002504 physiological saline solution Substances 0.000 description 2
- 150000003141 primary amines Chemical class 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 2
- 238000001959 radiotherapy Methods 0.000 description 2
- 238000010992 reflux Methods 0.000 description 2
- 229930195734 saturated hydrocarbon Natural products 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 238000003325 tomography Methods 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- 229960000281 trometamol Drugs 0.000 description 2
- 238000005292 vacuum distillation Methods 0.000 description 2
- CUGDYSSBTWBKII-LXGUWJNJSA-N (2r,3r,4r,5s)-6-(dimethylamino)hexane-1,2,3,4,5-pentol Chemical compound CN(C)C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO CUGDYSSBTWBKII-LXGUWJNJSA-N 0.000 description 1
- LNAZSHAWQACDHT-XIYTZBAFSA-N (2r,3r,4s,5r,6s)-4,5-dimethoxy-2-(methoxymethyl)-3-[(2s,3r,4s,5r,6r)-3,4,5-trimethoxy-6-(methoxymethyl)oxan-2-yl]oxy-6-[(2r,3r,4s,5r,6r)-4,5,6-trimethoxy-2-(methoxymethyl)oxan-3-yl]oxyoxane Chemical compound CO[C@@H]1[C@@H](OC)[C@H](OC)[C@@H](COC)O[C@H]1O[C@H]1[C@H](OC)[C@@H](OC)[C@H](O[C@H]2[C@@H]([C@@H](OC)[C@H](OC)O[C@@H]2COC)OC)O[C@@H]1COC LNAZSHAWQACDHT-XIYTZBAFSA-N 0.000 description 1
- 125000003161 (C1-C6) alkylene group Chemical group 0.000 description 1
- XJLSEXAGTJCILF-RXMQYKEDSA-N (R)-nipecotic acid zwitterion Chemical compound OC(=O)[C@@H]1CCCNC1 XJLSEXAGTJCILF-RXMQYKEDSA-N 0.000 description 1
- OGYGFUAIIOPWQD-UHFFFAOYSA-N 1,3-thiazolidine Chemical compound C1CSCN1 OGYGFUAIIOPWQD-UHFFFAOYSA-N 0.000 description 1
- RYHBNJHYFVUHQT-UHFFFAOYSA-N 1,4-Dioxane Chemical compound C1COCCO1 RYHBNJHYFVUHQT-UHFFFAOYSA-N 0.000 description 1
- FJLUATLTXUNBOT-UHFFFAOYSA-N 1-Hexadecylamine Chemical compound CCCCCCCCCCCCCCCCN FJLUATLTXUNBOT-UHFFFAOYSA-N 0.000 description 1
- PVOAHINGSUIXLS-UHFFFAOYSA-N 1-Methylpiperazine Chemical compound CN1CCNCC1 PVOAHINGSUIXLS-UHFFFAOYSA-N 0.000 description 1
- WGCYRFWNGRMRJA-UHFFFAOYSA-N 1-ethylpiperazine Chemical compound CCN1CCNCC1 WGCYRFWNGRMRJA-UHFFFAOYSA-N 0.000 description 1
- LISKJKUMLVQGKE-UHFFFAOYSA-N 1-morpholin-4-yl-2-piperazin-1-ylethanone Chemical compound C1COCCN1C(=O)CN1CCNCC1 LISKJKUMLVQGKE-UHFFFAOYSA-N 0.000 description 1
- PKDPUENCROCRCH-UHFFFAOYSA-N 1-piperazin-1-ylethanone Chemical compound CC(=O)N1CCNCC1 PKDPUENCROCRCH-UHFFFAOYSA-N 0.000 description 1
- HNVIQLPOGUDBSU-UHFFFAOYSA-N 2,6-dimethylmorpholine Chemical compound CC1CNCC(C)O1 HNVIQLPOGUDBSU-UHFFFAOYSA-N 0.000 description 1
- SDGKUVSVPIIUCF-UHFFFAOYSA-N 2,6-dimethylpiperidine Chemical compound CC1CCCC(C)N1 SDGKUVSVPIIUCF-UHFFFAOYSA-N 0.000 description 1
- MFYSUUPKMDJYPF-UHFFFAOYSA-N 2-[(4-methyl-2-nitrophenyl)diazenyl]-3-oxo-n-phenylbutanamide Chemical compound C=1C=CC=CC=1NC(=O)C(C(=O)C)N=NC1=CC=C(C)C=C1[N+]([O-])=O MFYSUUPKMDJYPF-UHFFFAOYSA-N 0.000 description 1
- ZKPOSKYDPFGCFP-UHFFFAOYSA-N 2-[2-[[2-(tert-butylamino)-2-oxoethyl]-(carboxymethyl)amino]ethyl-[2-[carboxymethyl-(2-ethoxy-2-oxoethyl)amino]ethyl]amino]acetic acid Chemical compound CCOC(=O)CN(CC(O)=O)CCN(CC(O)=O)CCN(CC(O)=O)CC(=O)NC(C)(C)C ZKPOSKYDPFGCFP-UHFFFAOYSA-N 0.000 description 1
- GGQCGYZMZZZWQI-UHFFFAOYSA-N 2-[2-[bis(carboxymethyl)amino]ethyl-[2-[carboxymethyl-[2-[(4-methoxyphenyl)methylamino]-2-oxoethyl]amino]ethyl]amino]acetic acid Chemical compound COC1=CC=C(CNC(=O)CN(CCN(CCN(CC(O)=O)CC(O)=O)CC(O)=O)CC(O)=O)C=C1 GGQCGYZMZZZWQI-UHFFFAOYSA-N 0.000 description 1
- QMOPWGUHCOMYNX-UHFFFAOYSA-N 2-[2-[bis(carboxymethyl)amino]ethyl-[2-[carboxymethyl-[2-oxo-2-(undecylamino)ethyl]amino]ethyl]amino]acetic acid Chemical compound CCCCCCCCCCCNC(=O)CN(CC(O)=O)CCN(CC(O)=O)CCN(CC(O)=O)CC(O)=O QMOPWGUHCOMYNX-UHFFFAOYSA-N 0.000 description 1
- JQRBBKFUEZDIRG-UHFFFAOYSA-N 2-hydroxy-1-piperazin-1-ylpropan-1-one Chemical compound CC(O)C(=O)N1CCNCC1 JQRBBKFUEZDIRG-UHFFFAOYSA-N 0.000 description 1
- RFVNOJDQRGSOEL-UHFFFAOYSA-N 2-hydroxyethyl octadecanoate Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCCO RFVNOJDQRGSOEL-UHFFFAOYSA-N 0.000 description 1
- YXPHKMZYEBWDQO-UHFFFAOYSA-N 2-methoxy-1-piperazin-1-ylethanone Chemical compound COCC(=O)N1CCNCC1 YXPHKMZYEBWDQO-UHFFFAOYSA-N 0.000 description 1
- NJBCRXCAPCODGX-UHFFFAOYSA-N 2-methyl-n-(2-methylpropyl)propan-1-amine Chemical compound CC(C)CNCC(C)C NJBCRXCAPCODGX-UHFFFAOYSA-N 0.000 description 1
- FFOHTSTWXWJGQR-UHFFFAOYSA-N 2-piperazin-1-yl-n-propan-2-ylacetamide Chemical compound CC(C)NC(=O)CN1CCNCC1 FFOHTSTWXWJGQR-UHFFFAOYSA-N 0.000 description 1
- LDSQQXKSEFZAPE-UHFFFAOYSA-N 2-piperidin-4-ylethanol Chemical compound OCCC1CCNCC1 LDSQQXKSEFZAPE-UHFFFAOYSA-N 0.000 description 1
- WNRGWPVJGDABME-UHFFFAOYSA-N 3,5-Dimethoxyaniline Chemical compound COC1=CC(N)=CC(OC)=C1 WNRGWPVJGDABME-UHFFFAOYSA-N 0.000 description 1
- HGSDPPKIASRECG-UHFFFAOYSA-N 3-hydroxy-1-piperazin-1-ylpropan-1-one Chemical compound OCCC(=O)N1CCNCC1 HGSDPPKIASRECG-UHFFFAOYSA-N 0.000 description 1
- JVQIKJMSUIMUDI-UHFFFAOYSA-N 3-pyrroline Chemical compound C1NCC=C1 JVQIKJMSUIMUDI-UHFFFAOYSA-N 0.000 description 1
- DPBWFNDFMCCGGJ-UHFFFAOYSA-N 4-Piperidine carboxamide Chemical compound NC(=O)C1CCNCC1 DPBWFNDFMCCGGJ-UHFFFAOYSA-N 0.000 description 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Natural products OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical class OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 description 1
- GAWIXWVDTYZWAW-UHFFFAOYSA-N C[CH]O Chemical group C[CH]O GAWIXWVDTYZWAW-UHFFFAOYSA-N 0.000 description 1
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- XBPCUCUWBYBCDP-UHFFFAOYSA-N Dicyclohexylamine Chemical compound C1CCCCC1NC1CCCCC1 XBPCUCUWBYBCDP-UHFFFAOYSA-N 0.000 description 1
- 229910052693 Europium Inorganic materials 0.000 description 1
- CWYNVVGOOAEACU-UHFFFAOYSA-N Fe2+ Chemical compound [Fe+2] CWYNVVGOOAEACU-UHFFFAOYSA-N 0.000 description 1
- 102000008100 Human Serum Albumin Human genes 0.000 description 1
- 108091006905 Human Serum Albumin Proteins 0.000 description 1
- 206010061216 Infarction Diseases 0.000 description 1
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 description 1
- HBBGRARXTFLTSG-UHFFFAOYSA-N Lithium ion Chemical compound [Li+] HBBGRARXTFLTSG-UHFFFAOYSA-N 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- WAEMQWOKJMHJLA-UHFFFAOYSA-N Manganese(2+) Chemical compound [Mn+2] WAEMQWOKJMHJLA-UHFFFAOYSA-N 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- BZORFPDSXLZWJF-UHFFFAOYSA-N N,N-dimethyl-1,4-phenylenediamine Chemical compound CN(C)C1=CC=C(N)C=C1 BZORFPDSXLZWJF-UHFFFAOYSA-N 0.000 description 1
- SECXISVLQFMRJM-UHFFFAOYSA-N N-Methylpyrrolidone Chemical compound CN1CCCC1=O SECXISVLQFMRJM-UHFFFAOYSA-N 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- VEQPNABPJHWNSG-UHFFFAOYSA-N Nickel(2+) Chemical compound [Ni+2] VEQPNABPJHWNSG-UHFFFAOYSA-N 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- WYNCHZVNFNFDNH-UHFFFAOYSA-N Oxazolidine Chemical compound C1COCN1 WYNCHZVNFNFDNH-UHFFFAOYSA-N 0.000 description 1
- 208000037273 Pathologic Processes Diseases 0.000 description 1
- NPYPAHLBTDXSSS-UHFFFAOYSA-N Potassium ion Chemical compound [K+] NPYPAHLBTDXSSS-UHFFFAOYSA-N 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 240000007651 Rubus glaucus Species 0.000 description 1
- 235000011034 Rubus glaucus Nutrition 0.000 description 1
- 235000009122 Rubus idaeus Nutrition 0.000 description 1
- FKNQFGJONOIPTF-UHFFFAOYSA-N Sodium cation Chemical compound [Na+] FKNQFGJONOIPTF-UHFFFAOYSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 241000193803 Therea Species 0.000 description 1
- 208000025865 Ulcer Diseases 0.000 description 1
- 230000003187 abdominal effect Effects 0.000 description 1
- 125000002777 acetyl group Chemical group [H]C([H])([H])C(*)=O 0.000 description 1
- 239000008186 active pharmaceutical agent Substances 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 238000005904 alkaline hydrolysis reaction Methods 0.000 description 1
- 125000003342 alkenyl group Chemical group 0.000 description 1
- 238000007098 aminolysis reaction Methods 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 150000001450 anions Chemical class 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 239000000010 aprotic solvent Substances 0.000 description 1
- 239000013011 aqueous formulation Substances 0.000 description 1
- 239000012736 aqueous medium Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000007900 aqueous suspension Substances 0.000 description 1
- 229960005070 ascorbic acid Drugs 0.000 description 1
- 235000010323 ascorbic acid Nutrition 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
- 239000012752 auxiliary agent Substances 0.000 description 1
- 229910052788 barium Inorganic materials 0.000 description 1
- DSAJWYNOEDNPEQ-UHFFFAOYSA-N barium atom Chemical compound [Ba] DSAJWYNOEDNPEQ-UHFFFAOYSA-N 0.000 description 1
- 229910001424 calcium ion Inorganic materials 0.000 description 1
- 125000001589 carboacyl group Chemical group 0.000 description 1
- 150000001718 carbodiimides Chemical class 0.000 description 1
- 150000004649 carbonic acid derivatives Chemical class 0.000 description 1
- 238000005341 cation exchange Methods 0.000 description 1
- 150000001767 cationic compounds Chemical class 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000013522 chelant Substances 0.000 description 1
- 229910052729 chemical element Inorganic materials 0.000 description 1
- BFGKITSFLPAWGI-UHFFFAOYSA-N chromium(3+) Chemical compound [Cr+3] BFGKITSFLPAWGI-UHFFFAOYSA-N 0.000 description 1
- XLJKHNWPARRRJB-UHFFFAOYSA-N cobalt(2+) Chemical compound [Co+2] XLJKHNWPARRRJB-UHFFFAOYSA-N 0.000 description 1
- 235000019628 coolness Nutrition 0.000 description 1
- 150000004696 coordination complex Chemical class 0.000 description 1
- 125000000113 cyclohexyl group Chemical group [H]C1([H])C([H])([H])C([H])([H])C([H])(*)C([H])([H])C1([H])[H] 0.000 description 1
- 125000001511 cyclopentyl group Chemical group [H]C1([H])C([H])([H])C([H])([H])C([H])(*)C1([H])[H] 0.000 description 1
- 230000000254 damaging effect Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 239000000032 diagnostic agent Substances 0.000 description 1
- 229940039227 diagnostic agent Drugs 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- ZBCBWPMODOFKDW-UHFFFAOYSA-N diethanolamine Chemical compound OCCNCCO ZBCBWPMODOFKDW-UHFFFAOYSA-N 0.000 description 1
- 125000004177 diethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 1
- HPNMFZURTQLUMO-UHFFFAOYSA-N diethylamine Chemical compound CCNCC HPNMFZURTQLUMO-UHFFFAOYSA-N 0.000 description 1
- 229940043279 diisopropylamine Drugs 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- LAWOZCWGWDVVSG-UHFFFAOYSA-N dioctylamine Chemical compound CCCCCCCCNCCCCCCCC LAWOZCWGWDVVSG-UHFFFAOYSA-N 0.000 description 1
- WEHWNAOGRSTTBQ-UHFFFAOYSA-N dipropylamine Chemical compound CCCNCCC WEHWNAOGRSTTBQ-UHFFFAOYSA-N 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 238000001839 endoscopy Methods 0.000 description 1
- JHFPQYFEJICGKC-UHFFFAOYSA-N erbium(3+) Chemical compound [Er+3] JHFPQYFEJICGKC-UHFFFAOYSA-N 0.000 description 1
- 125000004185 ester group Chemical group 0.000 description 1
- 150000002170 ethers Chemical class 0.000 description 1
- OGPBJKLSAFTDLK-UHFFFAOYSA-N europium atom Chemical compound [Eu] OGPBJKLSAFTDLK-UHFFFAOYSA-N 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- RJOJUSXNYCILHH-UHFFFAOYSA-N gadolinium(3+) Chemical compound [Gd+3] RJOJUSXNYCILHH-UHFFFAOYSA-N 0.000 description 1
- 229940083124 ganglion-blocking antiadrenergic secondary and tertiary amines Drugs 0.000 description 1
- 230000027119 gastric acid secretion Effects 0.000 description 1
- 230000001434 glomerular Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 125000003187 heptyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 150000002395 hexacarboxylic acids Chemical class 0.000 description 1
- 125000004051 hexyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
- SCKNFLZJSOHWIV-UHFFFAOYSA-N holmium(3+) Chemical compound [Ho+3] SCKNFLZJSOHWIV-UHFFFAOYSA-N 0.000 description 1
- 150000002430 hydrocarbons Chemical class 0.000 description 1
- 229960000443 hydrochloric acid Drugs 0.000 description 1
- 235000011167 hydrochloric acid Nutrition 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 description 1
- 150000004679 hydroxides Chemical class 0.000 description 1
- 125000004029 hydroxymethyl group Chemical group [H]OC([H])([H])* 0.000 description 1
- 239000005457 ice water Substances 0.000 description 1
- 125000002632 imidazolidinyl group Chemical group 0.000 description 1
- MTNDZQHUAFNZQY-UHFFFAOYSA-N imidazoline Chemical compound C1CN=CN1 MTNDZQHUAFNZQY-UHFFFAOYSA-N 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000007574 infarction Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 229910001411 inorganic cation Inorganic materials 0.000 description 1
- 238000001990 intravenous administration Methods 0.000 description 1
- SRJOCJYGOFTFLH-UHFFFAOYSA-N isonipecotic acid Chemical compound OC(=O)C1CCNCC1 SRJOCJYGOFTFLH-UHFFFAOYSA-N 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 230000003907 kidney function Effects 0.000 description 1
- TYQCGQRIZGCHNB-JLAZNSOCSA-N l-ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(O)=C(O)C1=O TYQCGQRIZGCHNB-JLAZNSOCSA-N 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 239000000787 lecithin Substances 0.000 description 1
- 235000010445 lecithin Nutrition 0.000 description 1
- 229910052744 lithium Inorganic materials 0.000 description 1
- 229910001416 lithium ion Inorganic materials 0.000 description 1
- 230000004807 localization Effects 0.000 description 1
- 230000005291 magnetic effect Effects 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 229910044991 metal oxide Inorganic materials 0.000 description 1
- 150000004706 metal oxides Chemical class 0.000 description 1
- 150000001455 metallic ions Chemical class 0.000 description 1
- 229940057952 methanol Drugs 0.000 description 1
- 229920000609 methyl cellulose Polymers 0.000 description 1
- 239000001923 methylcellulose Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 150000004682 monohydrates Chemical class 0.000 description 1
- 125000002757 morpholinyl group Chemical group 0.000 description 1
- ZXQKYQVJDRTTLZ-UHFFFAOYSA-N n,n-diethylpiperidine-3-carboxamide Chemical compound CCN(CC)C(=O)C1CCCNC1 ZXQKYQVJDRTTLZ-UHFFFAOYSA-N 0.000 description 1
- XGABIOSYJHFORX-UHFFFAOYSA-N n,n-dimethylpiperidine-4-carboxamide Chemical compound CN(C)C(=O)C1CCNCC1 XGABIOSYJHFORX-UHFFFAOYSA-N 0.000 description 1
- OBYVIBDTOCAXSN-UHFFFAOYSA-N n-butan-2-ylbutan-2-amine Chemical compound CCC(C)NC(C)CC OBYVIBDTOCAXSN-UHFFFAOYSA-N 0.000 description 1
- AGVKXDPPPSLISR-UHFFFAOYSA-N n-ethylcyclohexanamine Chemical compound CCNC1CCCCC1 AGVKXDPPPSLISR-UHFFFAOYSA-N 0.000 description 1
- UGQXPTUMTGDBJJ-UHFFFAOYSA-N n-methyl-n-piperidin-4-ylacetamide Chemical compound CC(=O)N(C)C1CCNCC1 UGQXPTUMTGDBJJ-UHFFFAOYSA-N 0.000 description 1
- QEFYFXOXNSNQGX-UHFFFAOYSA-N neodymium atom Chemical compound [Nd] QEFYFXOXNSNQGX-UHFFFAOYSA-N 0.000 description 1
- BVOCPVIXARZNQN-UHFFFAOYSA-N nipecotamide Chemical compound NC(=O)C1CCCNC1 BVOCPVIXARZNQN-UHFFFAOYSA-N 0.000 description 1
- 125000002347 octyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 150000002892 organic cations Chemical class 0.000 description 1
- 125000000160 oxazolidinyl group Chemical group 0.000 description 1
- BHAAPTBBJKJZER-UHFFFAOYSA-N p-anisidine Chemical compound COC1=CC=C(N)C=C1 BHAAPTBBJKJZER-UHFFFAOYSA-N 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- 230000005298 paramagnetic effect Effects 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 230000009054 pathological process Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 229960003330 pentetic acid Drugs 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- HZLFQUWNZMMHQM-UHFFFAOYSA-N piperazin-1-ylmethanol Chemical compound OCN1CCNCC1 HZLFQUWNZMMHQM-UHFFFAOYSA-N 0.000 description 1
- 125000004193 piperazinyl group Chemical group 0.000 description 1
- PRAYXGYYVXRDDW-UHFFFAOYSA-N piperidin-2-ylmethanol Chemical compound OCC1CCCCN1 PRAYXGYYVXRDDW-UHFFFAOYSA-N 0.000 description 1
- BIWOSRSKDCZIFM-UHFFFAOYSA-N piperidin-3-ol Chemical compound OC1CCCNC1 BIWOSRSKDCZIFM-UHFFFAOYSA-N 0.000 description 1
- HDOWRFHMPULYOA-UHFFFAOYSA-N piperidin-4-ol Chemical compound OC1CCNCC1 HDOWRFHMPULYOA-UHFFFAOYSA-N 0.000 description 1
- XBXHCBLBYQEYTI-UHFFFAOYSA-N piperidin-4-ylmethanol Chemical compound OCC1CCNCC1 XBXHCBLBYQEYTI-UHFFFAOYSA-N 0.000 description 1
- 125000003386 piperidinyl group Chemical group 0.000 description 1
- 125000005936 piperidyl group Chemical group 0.000 description 1
- 229920001983 poloxamer Polymers 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- BITYAPCSNKJESK-UHFFFAOYSA-N potassiosodium Chemical compound [Na].[K] BITYAPCSNKJESK-UHFFFAOYSA-N 0.000 description 1
- 229910001414 potassium ion Inorganic materials 0.000 description 1
- WCWKKSOQLQEJTE-UHFFFAOYSA-N praseodymium(3+) Chemical compound [Pr+3] WCWKKSOQLQEJTE-UHFFFAOYSA-N 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- HVVNJUAVDAZWCB-UHFFFAOYSA-N prolinol Chemical compound OCC1CCCN1 HVVNJUAVDAZWCB-UHFFFAOYSA-N 0.000 description 1
- 125000004368 propenyl group Chemical group C(=CC)* 0.000 description 1
- 125000001501 propionyl group Chemical group O=C([*])C([H])([H])C([H])([H])[H] 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- USPWKWBDZOARPV-UHFFFAOYSA-N pyrazolidine Chemical compound C1CNNC1 USPWKWBDZOARPV-UHFFFAOYSA-N 0.000 description 1
- 125000003072 pyrazolidinyl group Chemical group 0.000 description 1
- DNXIASIHZYFFRO-UHFFFAOYSA-N pyrazoline Chemical compound C1CN=NC1 DNXIASIHZYFFRO-UHFFFAOYSA-N 0.000 description 1
- 125000002755 pyrazolinyl group Chemical group 0.000 description 1
- JHHZLHWJQPUNKB-UHFFFAOYSA-N pyrrolidin-3-ol Chemical compound OC1CCNC1 JHHZLHWJQPUNKB-UHFFFAOYSA-N 0.000 description 1
- 125000000719 pyrrolidinyl group Chemical group 0.000 description 1
- 125000001422 pyrrolinyl group Chemical group 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 239000012429 reaction media Substances 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- DOSGOCSVHPUUIA-UHFFFAOYSA-N samarium(3+) Chemical compound [Sm+3] DOSGOCSVHPUUIA-UHFFFAOYSA-N 0.000 description 1
- 238000007127 saponification reaction Methods 0.000 description 1
- 150000003335 secondary amines Chemical class 0.000 description 1
- 125000000467 secondary amino group Chemical group [H]N([*:1])[*:2] 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 229910001415 sodium ion Inorganic materials 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- HKCRVXUAKWXBLE-UHFFFAOYSA-N terbium(3+) Chemical compound [Tb+3] HKCRVXUAKWXBLE-UHFFFAOYSA-N 0.000 description 1
- 150000003512 tertiary amines Chemical class 0.000 description 1
- YLQBMQCUIZJEEH-UHFFFAOYSA-N tetrahydrofuran Natural products C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 125000001984 thiazolidinyl group Chemical group 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 230000008733 trauma Effects 0.000 description 1
- 231100000397 ulcer Toxicity 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- QFKMMXYLAPZKIB-UHFFFAOYSA-N undecan-1-amine Chemical compound CCCCCCCCCCCN QFKMMXYLAPZKIB-UHFFFAOYSA-N 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- ORZHVTYKPFFVMG-UHFFFAOYSA-N xylenol orange Chemical compound OC(=O)CN(CC(O)=O)CC1=C(O)C(C)=CC(C2(C3=CC=CC=C3S(=O)(=O)O2)C=2C=C(CN(CC(O)=O)CC(O)=O)C(O)=C(C)C=2)=C1 ORZHVTYKPFFVMG-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
ABSTRACT OF THE DISCLOSURE
---------------Complex compounds of general Formula I
(I) wherein n means the number 0, 1 or 2, R1 and R2 are independently hydrogen atoms, lower alkyl groups, phenyl groups, benzyl groups or, if n is the number 0, also jointly a trimethylene or a tetramethylene group, R3 is a saturated, unsaturated, straight-chain or branched-chain or cyclic aliphatic hydrocarbon residue of up to 16 carbon atoms or, if R4 is a hydrogen atom, a cycloalkyl group or an aryl or aralkyl group optionally substituted by one or several di-C1-C6-alkylamino groups or by one or several C1-C6-alkoxy groups, R4 is a hydrogen atom, a saturated, unsaturated, straight-chain or branched-chain or cyclic hydrocarbon residue of up to 16 carbon atoms, or R3 and R4 jointly mean a saturated or unsaturated 5- or 6-member ring which is optionally substituted by one or several C1-C6-alkyl, C1-C5-hydroxyalkyl residues, an optionally hydroxylated or Cl-C6-alkoxylated C2-C6-acyl residue, a hydroxy residue, a carbamoyl residue, a carbamoyl-substituted Cl-C6-alkyl residue, a carbamoyl residue substituted on the carbamoyl nitrogen by one or two Cl-C6-alkyl residue(s) -- which can also form a ring containing, if desired, an oxygen atom -- or a C1-C6-acylamino or C1-C6-alkylamino residue, this 5- or 6-membered ring optionally containing a further nitrogen, oxygen or sulfur atom, or a carbonyl group, X is a hydrogen atom and/or a metal ion equivalent of at least one element of atomic numbers 21-29, 42, 44 or 58-70, Y is a COOX- or group, with the proviso that at least two of the substitu-ents X stand for a metal ion equivalent, as well as their salts with organic and/or inorganic bases, are valuabe compounds for the organ-specific NMR diagnostics and in case of patients with renal insufficiency.
.1.
---------------Complex compounds of general Formula I
(I) wherein n means the number 0, 1 or 2, R1 and R2 are independently hydrogen atoms, lower alkyl groups, phenyl groups, benzyl groups or, if n is the number 0, also jointly a trimethylene or a tetramethylene group, R3 is a saturated, unsaturated, straight-chain or branched-chain or cyclic aliphatic hydrocarbon residue of up to 16 carbon atoms or, if R4 is a hydrogen atom, a cycloalkyl group or an aryl or aralkyl group optionally substituted by one or several di-C1-C6-alkylamino groups or by one or several C1-C6-alkoxy groups, R4 is a hydrogen atom, a saturated, unsaturated, straight-chain or branched-chain or cyclic hydrocarbon residue of up to 16 carbon atoms, or R3 and R4 jointly mean a saturated or unsaturated 5- or 6-member ring which is optionally substituted by one or several C1-C6-alkyl, C1-C5-hydroxyalkyl residues, an optionally hydroxylated or Cl-C6-alkoxylated C2-C6-acyl residue, a hydroxy residue, a carbamoyl residue, a carbamoyl-substituted Cl-C6-alkyl residue, a carbamoyl residue substituted on the carbamoyl nitrogen by one or two Cl-C6-alkyl residue(s) -- which can also form a ring containing, if desired, an oxygen atom -- or a C1-C6-acylamino or C1-C6-alkylamino residue, this 5- or 6-membered ring optionally containing a further nitrogen, oxygen or sulfur atom, or a carbonyl group, X is a hydrogen atom and/or a metal ion equivalent of at least one element of atomic numbers 21-29, 42, 44 or 58-70, Y is a COOX- or group, with the proviso that at least two of the substitu-ents X stand for a metal ion equivalent, as well as their salts with organic and/or inorganic bases, are valuabe compounds for the organ-specific NMR diagnostics and in case of patients with renal insufficiency.
.1.
Description
,; ` `. "' '. fi S
US~ OF AMIDE COMPLEX COMPO~DS
Cross-Reference to Related Ap~lications This application is a continuation-in-part of Serial No. 07/495,803, filed March 14, 1990, which is a 5 continuation of Serial No. 07/100,6~, filed September 24, 1987 (abandoned), both of which are entirely incorporated by reference herein.
In addition, this application is related to U.S.
Application Serial Nos. 07/078,~07 (filed July 28, 1987), 07/063,355 (filed June 18, 1987), 07/020,301 (filed March 2, 1987), 07/020,300 (filed ~arch 2, 1987), 07/020,993 (filed March 2, 19~7), 07/020,992 (filed March 2, 1987), 06/936,055 (filed November 28, 1986), 06/876,497 (filed June 20, 1986), and 06~627,143 (filed July 2, 1984), each of which is a divisional, continuation or C-I-P of - 06/573,184 (filed January 23, 1984), now U.S. Patent No.
4,647,447, which is a C-I-P of 06/401,594 (filed July 26, 1982), and this application is a C-I-P of all of said applications directly or indirectly and all of which applications are entirely incorporated by reference herein.
Summary of the Invention This invention relates to physiologically compatible amide complex compounds and the use there~f in the production of agents for organ-specific N~R diagnostics and for cases involving patients with ren~l insufficiency, as well as NMR msthods utilizing these compounds.
European Patent Application Publication Number 263,059 claims compounds of Formula I R3 Y--CH2 CH2CO--N~ 4 N - -- CH--(CH2~1 -CH2) n CIH I (I) XOOCCH2 R CHzCOOX R CH2COOX
wherein nis o, 1 or 2, 10 Rl and R2 independently are hydrogen atoms, lower alkyl groups, phenyl groups, benzyl groups or, if n is O, jointly can also form a trimethylene or a tetramethylene group, R3 is a saturated, unsaturated, straight-chain or branched-chain or cyclic aliphatic hydrocarbon residue of up to 16 carbon atoms and, if R4 is a hydrogen atom, at least one R3 :i.s a cycloalkyl group, or an aryl or aralkyl group optionally substituted by one or several di C1-C6-alkylamino groups or by one or several C1-C6-alkoxy groups, R4 is a hydrogen atom, or a saturated, unsaturated, straight-chain or branched-chain or cyclic hydrocarbon residue of up to 16 carbon atoms, or R3 and R4 jointly form a satuxated or uns~turated 5- or 6-membered ring which is option~lly substituted by one or several of C1-C6-alkyl, C1-C5-hydroxy-alkyl, optionally hydroxylated ~r C1-C6-alkoxy-lated Cz-C6-acyl, hydroxy, carbamoyl, carbamoyl-substituted C1-C6 alkyl residue, carbamoyl substituted on the carhamoyl nitrogen by one or two C1-C6-alkyl residue(s) -- which can also form a ring optionally containing an oxy-gen atom -- or C1-C6-acylamino or C1-C6-alkyl-amino;
this 5- or 6-membered ring opti~nally containing a furkher nitrogen, ~xygen or sulfur atom, or carbonyl group, X means a hydrogen atom and/or a metal ion equivalent, R 3 Y is a COOX or ~ON\ group, as well as their salts with organic and/or inorganic bases.
Compounds havirlg the anion of one oF
these complex-forming amides and one or several central ions of an element of atomic numbers 21-29, 31, 32, 38, 39, 42-44, 49, 57-83 and optionally one or several cations of an inorganic and/or organic base or amino acid are suited for the production of NMR, X-ray and radiology diagnostic media.
It has now been found that surprisingly an unexpected pharmacokinetic behavior is displayed by compounds of this general Formula I if these compounds contain at least one element of atomic numbers 21-29, 42, 44 or 58-70, i.e.,at least two of the substituents X must stand for a metal ion equivalent of these ele-ments, but in particular compounds of general Formula I wherein n is the number 1, R a n d R are hydrogen atoms, R3 is a saturated, unsaturated, straight-chain or branched-chain or cyclic aliphatic hydrocarbon residue of u~ to 16 carbon atoms and, if R is a hydrogen atom, at least one R3 is a cycloalkyl group, or an aryl or aralkyl group optionally substituted by one or several di-C1-C6-alkylamino groups or by one or several C1-C6-alkoxy groups, R4 is a hydrogen atom or a saturated, unsaturated, straight-chain, branched-chain or cyclic hydrocarbon residue of up to 16 carbon atoms, X is a hydrogen atom and/or a metal ion equivalent of at least one element of atomic numbers 21-29, 42, 44 or 58-70, R
/
Y is a COOX or CON~ group, R
with the proviso that at least two of the substituents X stand for a metal ion equivalent, as well as their salts with organic and/or inorganic bases.
Thus, for example, MAGNEVIST ~, thus far the only NMR contrast medium permitted worldwide, is distributed upon intravenous injection in an extra-cellular fashion and is excreted via the kidneysby glomerular secretion. Passage of intact cell membranes and extrarenal excretion are practically not at all observed.
MAGNEVIST ~ is especially well suited for the diagnosis of pathological regions (for example, inflammations, tumors, infarctions, etc.).
Contrast media exhibiting an at least partial extrarenal excretion would be desirable, especially for patients with limited kidney function (renal insufficiency) where MAGNEVIST G~is excreted only very slowly and, in part, can be removed from the organism only with the aid of a dialysis device.
r~ J ~ J
Consequently, there is a need ~or NMR contrast media e~hibiting a di~ferent pharmacokinetic behavior and thus higher organ specificity than MAGNEVIST~.
Accordingly, it is an object o~ this invention to provide such compounds and media and methods of using same.
Upon further study of the specification and appended claims, further objects and advantages of this invention will become apparent to those skilled in the art.
It has been found that the above-mentioned compounds surprisingly show the desired property:
renal elimination as well as excretion with the feces.
Surprisingly, elimination via the gallbladder, however, is not the only extrarenal path of elimination:
in NMR studies on rats, upon intravenous administration of the compounds of this invention, a contrast enhance-ment of the gastrointestinal tract has also been unexpectedly observed. The kidneys, as well as implanted tumors, are likewise visualized with improved contrast.
Elimination ~secretion) by way of the stomach ~` has the advantage that contras-ting of abdominal struc-tures (e.g. pancreas) from the gastrointestinal tract is made possible, with a simultaneous contrast enhance-ment of pathological processes (tumors, inflammations).
Imaging of the renal system, of the liver and gallbladder, and the bile ducts can moreover likewise be achieved.
Besides the improved visualization of ulcers and stomach carcinomas, it is also possible to perform studies on gastric acid secretion with the aid of imaging procedures.
Accordingly, by making the compounds of this invention available, help can be e~tended to patients with renal insufficiency as well as patients suffering from gastrointestinal disorders (at least 10% of the population in the Western industrial countries).
patients suspected of harboring such disease, must submit to diagnos-tic tests. At present, two methods suitable fo~ this purpose are utilized above all:
endoscopy and X-ray diagnos-tics with the aid of barium contrast media.
These tests exhibit various drawbacks: they carry the risk of radiation stress, cause trauma, are connected with inconveniences, occasionally even with risks for the patient, and thus can evoke psychological stress. In most cases, these tests must be repeated;
their performance is relatively complicated, require the patient's active cooperation (e.g. assumption of a specific bodily attitude) and frequently cannot be employed in case of frail and high-risk patients.
The object of providing novel diagnostic methods for the identification and localization of gastrointestinal diseases, which methods do not exhibit these drawbacks, has thus likewise been attained by the complex compounds and agents as mentioned above.
Their pharmacokinetics permit, even wi-thout specific measures, an improvement in the diagnosis of numerous diseases. The complexes for the most part are excreted again in unchanged form and rapidly so that, especially also in case of using relatively toxic metallic ions, no damaging effects are observed even at high dosage.
The practical use of the novel ccmplexes is also facilitated by their favorable chemical stability.
~-" f ~ r ," ~
Compounds of formula I, wherein X is hydrogen, are called "complexing agents", and those wherein at least two of the substituents X are a met~l ion equivalent are called "metal complexes~'.
For use in NMR diagnostics, the central ion of the complex salt will be paramagnetic. These are, in particular, the divalent and trivalent ions of the elements of atomic numbers 21-29, 42, ~4 and 58-70.
Suitable ions include, for example, the chromium(III), manganese(II), iron(II), cobalt(II), nickel(II), copper(II), praseodymium(III), neodymium(III), samarium(III) and ytterbium(III) ions. On account of their very strong magnetic moment, the gadolinium(III~, terbium(III), dysprosium(III), holmium(III); erbium(III) and iron(III) ions are espscially preferrad.
Suitable alkyl substituents R1 and R2 are hydrocarbons of 1-8, preferably 1-4 carbon atoms, such as, for example, methyl, ethyl, propyl, isopropyl, n-, sec- or tert.-butyl, isobutyl, and all is~mers of pentyl, hexyl, heptyl and octyl.
Suitable aliphatic substituents R3 and R4 are saturated (e.g., alkyl), unsaturated (e.g., alkenyl), straight-chain or branched-chain or cycli- hydrocarbons of up to 16 carbon atoms, pre~erably 1-10 C atoms, most preferably saturated hydrocarbons of 1-10 carbon atoms, especially saturated hydrocarbons of 1-5 -arbon atoms.
Examples include methyl, ethyl, propyl, i~opropyl, butyl, isobutyl, pentyl, cyclopentyl, cyclohexyl, propenyl, etc.
Other suitable groups are the other alkyl groups mentioned above for R1 and R2 and isomers containing 9-16 C atoms as well as their ~lkenyl counterparts.
When R4 is a hydrogen atom, at :east one R3 is preferably C6-C10-aryl or C6-C10-Ar-C1-C6-alcyl group, e.g., phenyl or benzyl group, optionally substituted by one or several ~e.g., up to three) di-C1- to C6-alkylamino groups , or by one or several (e.g., up to three) C1- to C6-alkoxy groups.
In addition, when ~' i6 a hydrogen atom, R3 can also preferably be a cycloalkyl group, as mentioned above. The cycloalkyl group generally contains 3-16 carbon atoms, preferably ~-7 carbon atoms.
The heterocyclic 5- or 6-membered ring formed by R3 and R4 with inclusion of the amide nitrogen can be saturated, unsaturated andjor substituted and can optionally contain a nitrogen, oxygen or sulfur atom or carbonyl group.
The heterocycle can be substituted by hydroxy, C1-C6-alkyl, e.g., methyl, ethyl, propyl, isopropyl, butyl, C1-C5-hydroxyalkyl, e.g., hydroxymethyl, hydroxyethyl, or by C2-C6-acyl (e.g., alkanoyl), for example acetyl, propionyl, which can, if desired, be substituted by hydroxy or C1-C6-alkoxy, e.g., methoxy, ethoxy, etc.
A further substituent that can be mentioned is carbamoyl, linked to the heterocycle dire-tly or separaked by a C1-C6-alkylene group, for example methylene, ethylene, propylene, and whicn can also be substituted at the nitrogen, if desired, by one or two C1-C6-alkyl residue(s), e.g., methyl, ethyl, propyl, isopropyl, ~tc. The alkyl groups can, optionally, form a ring, such as, for example, a pyrrolidine or piperidine ring. The carbamoyl nitrogen can also be part of a morpholine ring, i.e., the latter ring can have an 0 atom.
Another possible substituent on the heterocycle that can be mentioned is an optionally C1-C6-alkylated or C1-C6-acylated (e.g., alkanoylated~ primary or secondary amino group, such as, for example, the methyl-, ethyl-, acetyl-, propionyl-, amino-, etc., group.
If the heterocycle is substituted, the total number of substituents is l to 3.
t ~
Suitable heterocycles are, for example: the pyrrolidinyl, piperidyl, pyrazolidinyl; pyrrolinyl, pyrazolinyl, piperazinyl, morpholinyl, imidazolidinyl, oxazolidinyl, thiazolidinyl rings.
If not all of the acidic hydrogen atoms are substituted by the central ion, then one, several, or all remaining hydrogen atom(s) can be replace~ by cations of inorganic and/or organic bases or amino acids. Suitable inorganic cations include, for example, the lithium ion, the potassium ion, the calcium ion and, in particular, the sodium ion. Suitable cations of organic bases include, inter alia, those of primary, secondary or tertiary amines, e.g., ethanolamine, diethanolamine, morpholine, glucamine, N,N-dimethylglucamine and especially N-methylglucamine. Suitable c~tions of amino acids include, for example, those of lysine, of arginine, and of ornithine.
Introduction of amide groups for the production of the complexing agents, i.e., of compounds of general Formula I wherein X means hydrogen, takes place by conventional partial conversion of activated carboxyl groups into amide groups of the respectively suited - tetra-, penta- and hexacarboxylic acids -- in correspondence with the desired final pro~uct. All of the synthesis pathways known to a person skilled in the art are suitable for this procedure.
One example is the reaction of -the anhydrides or esters of gener~l E~ormulae II, IV, V and VI:
VOC-C~ ~CH2-COV
N-Cj H- ( CH2- 1 -CH2 ) n-CI H-rl \ ( II ) ZOC-CH2 Rl CH2COOH R2 C~z-COZ
HOOC-C~ ~CH2-CO~l / N-CH- ( CH2-N-CH2 ) -CH-N \ ( IV ) R500C-CH2 Rl CH2COOH R CH -COZ
HOOC-CH2 ~ CH2COOH
~ N-CH-(CH2-N-CH2)n-~H-N ~ (V) R500C-CH2 Rl CH2COOH ~2 CH2COOH
HQQC- CH `
.j / \
N -CH - t CH2-N -CH2 ) n-CH -N ( V I ) .
R OOC-CH2 ¦1 CH2COOH R ~~
wherein Rl, R2 and n have the above-mentioned meanings, V and Z jointly mean an oxygen atom, or V is a hydroxy group and Z is the group.ing oR5~ wherein R is a C1~C6~a1ky1 residue, hs ~.3 .'; C.J ~ J
with amines oE general Formula III
HN ~ I I I ), wherein ~3 and R4 have the meanings given above.
Examples of suitable amines include: dimethyl-amine, diethylamine, di-n-propylamine, diisopropylamine, di-n-butylamine, diisobutylamine, di-sec-butylamine, N-methyl-n-propylamine, dioctylamine, dicyclohexyl-amine, N-ethylcyclohexylamine, diisopropenylamine, benzylamine, aniline, 4-methoxyaniline, 4-dimethyl-aminoaniline, 3,5-dimethoxyaniline, 4-methoxybenzyl-amine, morpholine, pyrrolidine, piperidine, N-methyl-piperazine, N-ethylpiperazine, N-(2-hydroxyethyl~-piperazine, N-(hydroxymethyl)piperazine, piperazino-acetic acid isopropylamide, N-(piperazinomethyl-carbonyl)morpholine, N-(piperazinomethylcarbonyl)pyr-rolidine, 2-(2-hydroxymethyl)piperidine, 4-(2-hydroxy-ethyl)piperidine 7 2-hydroxymethylpiperidine, 4-hydroxymethylpiperidine, 2-hydroxymethylpyrrolidine, 3-hydroxypiperidine r 4-hydroxypiperidine, 3-hydroxy-pyrrolidine, 4-piperidone, 3-pyrroline, piperidine-3-carboxylic acid amide, piperidine-4-carboxylic acid amide, piperidine-3-carboxylic acid diethylamide, piperidine-4-carboxylic acid dimethylamide, 2,6-dimethylpiperidine, 2,6-dimethylmorpholine, N-acetyl-piperazine, N-(2-hydroxypropionyl)piperazine, N-(3-hydroxypropionyl)piperazine, N-(methoxyacetyl)-piperazine, 4-(N-acetyl-N-methylamino)piperidine, -;
~Z~ u ~-1 ,J ~.., ,~
piperidine-4-carboxylic acid (3-oxapentamethylene)amide, piperidine-3-carboxylic acid 13-oxapentamethylene)amide, N-~N',N'-dimethylcarbamoyl3piperazine, pyrazoline, pyrazolidine, imidazoline, oxazolidine, thiazolidine, etc.
The saponification of any ester groups that may still be present takes place according to methods known to one skilled in ~he art, for example,by alkaline hydrolysis.
The acid anhydrides of general Formula II
can be prepared conventionally, for example in accord-ance with the mode of operation disclosed in U.S.
Patent 3,660,388 or DOS 1,695,050, with acetic anhydride in pyridine. However, in certain instances,it is especially advantageous to conduct the lS step of splitting off water in a gentle fashion with carbodiimides in a suitable solvent, such as, for ex-ample dimethylformamide or dimethylacetamide.
The preparation of the monoanhydrides of gen-eral Formula VI will be described by using as the example the monoanhydride of diethylenetriaminepenta-acetic acid ethyl ester starting with the monoethyl ester of DTPA (J. Pharm. 5ci. 68 : 194, 1979):
N3-(2,6-Dioxomorpholinoethyl)-N6-(ethoxycarbonyl-methyl)-3,6-diazaoctanedioic Acid _________________________________________________ A suspension of 21.l g (50 millimoles) of N3,N6-bis(carboxymethyl)-N9~(ethoxycarbonylmethyl)-3,6,9-triazaundecanedioic acid in 250 ml of acetic anhydride is agitated for 3 days at room temperature after adding 42.2 ml of pyridine. Then the precip-itate is suctioned off, washed three times with re-spectively 50 ml of acetic anhydride and subsequently ~,J i~ ~.,J ~ ~, 3 ~1 - 13 ~
stirred for several hours with absolute diethyl ether.
Af-ter suctioning off the product, washing same with absolute diethyl e~her and drying under vacuum at 40 C, 18.0 g (= 89% of theory) of a white powder is obtained, mp 195-196 C.
Analysis (based on anhydrous substance):
Calculated: C 47.64 ll 6.25 N 10.42 Found: C 47.54 El 6.30 N 10.22 The reaction of the acid anhydrides to the amides can be performed in the liquid phase. Suitable reaction media include, for example, water, dipolar aprotic solvents, such as acetonitrile, N-methylpyrrolidone, dimethylformamide, dimethylacetamide, and the like, or mixtures thereof. The reaction temperatures range between about 0 C and 100 C, temperatures of ~0-80 C being preferred. The reaction periods xange between 0.5 hour and 2 days, preferably between 1 hour and 36 hours.
The esters of general Formula V are produced conventionally, for example according to the processes described in R.A. Guilmette et al., J. Pharm~ Sci.
68 : 194 (1979).
Aminolysis of the esters takes place in the liquid phase, for example in a suitable higher-boiling solvent, such as dimethylformamide, dimethylacetamide or dimethyl sulfoxide. The reaction temperatures are around 20 C to 200 C~ temperatures of 100-180 C
being preferred. The reaction times range between 2 hours and 2 days, reaction periods of between 4 houxs and 36 hours being the preferred ones.
~ J~3~
1 'I -Moreover, all methods known to a person skilled in the art for converting carboxyl groups into amide groups can be employed for the synthesis of the complex-ing agents of Formula I according to this invention, For example, the method by Krejcarek and Tuc~er, Biochm. Biophys. Res. Comrnun. 77 : 581 (1977) via mixed anhydrides.
The resultant compounds of Formula I
wherein X is a hydrogen atom represent complex-forming media. They can be isolated and purified, or they can be converted without isolation into metal complexes of general Formula I wherein at least two of the substi-tuent X mean a metal ion equivalent.
The metal complexes are prepared conven~ionally by the methods disclosed in Patents EP 71564, EP 130934 and DOS
3,401,052,by dissolving or suspending the metal oxide or a metal salt (e.g.,the nitrate, acetate, carbonate, chloride or sulfate) of the element of atomic numbers 21-Z9, 42, 44 or 58-70 in water and/or a lower alcohol (such as methanol, ethanol or isopropanol) and reacting with a solution or suspension of the equivalent amount of the complex-forming acid of Formula I wherein X means a hydrogen atom and subsequently, if desired, substituting any acidic hydrogen atoms of acid groups present by cations of inorganic and/or organic bases or amino acids.
Neutralization is herein effected with the air of inorganic bases (for example, hydroxides, carbonates or bicarbonates) of, for example, sodium potassium, lithium and/or organic bases such as, inter alia, primary, secondary and tertiary amines, such as, for example, ethanolamine, morpholine, glucamine, N-methyl- and N,N-dimethylglucami~e, as well as basic amino acids, such as, for example, lysine, arginine and ornithine.
f~J ~ 2 s~
In order to prepare the neutral complex com-pounds, it is possible, for example, to add to the acidic complex salts in an aqueous solution or suspen-sion such an amount of the desired bases that the neutral point is reached. The resultant solution can subsequently be evaporated to dryness under vacuum.
It is frequently advantageous to precipitate the thus-formed neutral salts by adding water-miscible solvents, such as, for example, lower alcohols (methanol, e-thanol, isopropanol, etc.), lower ketones (acetone etc.), polar ethers ~tetrahydrofuran, dioxane, 1,2-dimethoxy-ethane, etc.), and to obtain in this way crystallized products which can be readily isolated and easily purified. It has proven to be especially advantageous t~
add the desired base to the reaction mixture as early as during the complexing reaction, thereby saving a process step.
If the acidic complex compounds contain several free acidic groups, then it is frequently ex-pedient to prepare neutral mixed salts containinginorganic as well as organic cations as the counterions.
This can be done, for example, by reacting the complexing acid in an aqueous suspension or solu-tion with the oxide or salt of the element yieldin~
the central ion and with half the amount of an organic base needed for neutralization, isolating the thus-formed complex salt, purifying same if desired, and then combining same for complete neutralization with the required amount of inorganic base. The sequence of adding the bases can also be reversed.
The diagnostic media are likewise produced in a manner known per se by suspending or dissolving the complex compounds of this invention -- optionally after adding the additives customary in galenic pharmacy -- in an aqueous medium and subsequently , . ' " ,~`. !,; ` C " ' ~
sterilizing the suspension or solution, if desired.
Suitable additives are, for example, physiologically acceptable buffers (such as, e.g., trome-thamine), small additions of complexing agents (such as, e.g., diethylene-triaminepentaacetic acidj or, if necessary, electrolytessuch as, for example, sodium chloride or, if needed antioxidants, such as ascorbic acid, for example.
If, for enteral administration or other purposes, suspensions or solutions of the media of this invention in water or a physiological saline solu-tion are desirable, they can be mixed with one or several auxiliary agents customary in galenic pharmacy (for example methylcellulose, lactose, mannitol) and/or tensides (e.g., lecithins, TWEENS(~), MYRJ(~).and/or lS flavoring substances for taste improvement (e.g.
ethereal oils).
In principle, it is also possible to prepare the diagnostic media of this invention even without isolation of the complex salts. In any event, special care must be directed toward effecting the chelate formation so that the salts and salt solutions accord-ing to this invention are practically devoid of toxically active metal ions that are not complexed.
This can be ensured, for example, with the aid of color indicators, such as xylenol orange, by control titrations during the manufacturing process.
Consequently, the invention also relates to processes for preparing the complex compounds and their salts.
The final safety feature resides in purification of the isolated complex salt.
For nuclear spin tomography diagnostics in accord-ance with this invention, the diagnostic media are ad-ministered in a dosage of 1 ~mol/kg to 5 mmol/kg, prefer-ably 10 ~mol to 0.5 mmol/kg of the complex sa3t according to the invention. rn case of intravenous injection, aque-ous formulations are ussd with a concentr~tion of 50 ~mol/l to 2 mol/l, preferably 100 mmol/l to 1 mol/l. Rec-tal as well as oral administration is preferably per-formed with solutions of a concentration ~f 0.1 mmol to 100 mmol/l. The volumes administered ran~e from about 5 ml to 2 1, in dependence on the diagnostic problem.
Thus, the diagnostic media are intended for enteral and parenteral administration to mammals, including humans.
The diagnostic media fulfill thQ variegated requirements for suitability as contrast media for nuclear spin tomography. Thus, they are ~xcellently suited, upon oral or parenteral administration, for improving the information content of the image obtained with the aid of the nuclear spin tomograph, by increasing the signal intensity. They show furthermore the high efficacy necessary for burdening the body with minimal amounts of foreign substances, and the go3d compatibility required for maintaining the noninvasive -haracter of the tests.
The high water solubility of the diagnostic media permits production of highly concentrated solutions so that the volume load on the circulation is maintained within tolerable limits and dilution by b~dy fluids is compensated, i.e., NMR diagnostic agents must show 100 to 1000 times the water solubility of that f~r in vitro NM~
spectroscopy. Furthermore, the agents of this invention display not only high stability so that r~lease or ex-change of the -- toxic per se -- ions not covalently bound to the complexes takes place only e~tremely gra-dually within the time wherein the contra~t media are again entirely eliminated.
The agents of this invention can also be utilized for radiation therapy. Thus, complexes of gadolinium are excellently suited for neutron capture therapy due to the large capture cross section. If the medium of this invention is intended for use in the ver-sion of radiation therapy proposed by R.L. Mills et al.
[Nature 336 : 787 (1988)~, then the centr~l ion must be derived from a Mossbauer isotope, such as, for example, s7Fe or ls1Eu In their administration, the ag3nts of this invention can also be given together with a suitable vehicle, such as, for example, serum or physiological saline solution and/or together with a protein, such as, for example, human serum albumin. The dosage herein is dependent on the type of cellular disorder and on the properties of the metal complex utilized.
Consequently, the objective has been achieved over-all of opening up novel possibilities in diagnostic medicine by means of the recited complex -ompounds.
Without further elaboration, it is believed that one skilled in the art can, using th~ preceding description, utilize the present invention to its fullest extent. The following preferred specific embodiments are, therefore, to be construed as merely illustrative, and not limitative of the remainder of the disclosure in any way whatsoever.
In the foregoing and in the following examples, all temperatures are set forth uncorrected in degrees Celsius and unless otherwise indicated, all parts and percentages are by weight.
The entire disclosures of all applic~tions, patents and publications, cited above and below, ~nd of corresponding application Federal Republi- of Germany P 39 27 444.6, filed August 16, 1989, are hereby incorporated by reference.
(~
Example 1 (a) 6-Carboxymethyl-3-ethoxycarbonylme-thyl-9-phenyl-aminocarbonylmethyl-3,6,9-triazaundecanedioic Acid __________________________________________________ At 0 C, 2.42 g (6 mmol) of N3-(2,6-dioxo-morpholinoethyl)-N6-(ethoxycarbonylmethyl)-3,6-diaza-octanedioic acid is combined in DMF with 4.16 ml (3.04 g, 30 mrnol~ of triethylamine and 559 mg (6 mmol) of aniline and stirred overnight at room temperature.
Then the clear solution is concentrated under vacuum and the residue chromatoyraphed on silica gel with dichloromethane/methanol/acetic acid/water (5:3:1:1) as the mobile phase. The combined fractions are passed over approximately 10 ml of "Amberlite" IR 120 (H+ form) and the acidic eluate is concentrated.
Yield: 2.35 g (79~) Calculated: C 53.21 H 6.50 N 11.29 Found: C 53.01 H 6.55 N 11.26 (b) 3,6,9-Tris(carboxymethyl)-3,6,9-triazaundecane-dioic Acid Phenyl Monoamide _____._________________________________________ 1.5 g (3 mmol) of the ethyl ester described in Examplel(a) is dissolved in 2N NaOH and stirred for 2 hours at room temperature. A pH of 7 is set by addin~
"Amberlite" IR 120 (H form), the mixture is filtered off, and the neutral solution is passed over about 16 ml of "Amberlite" IR 120 (H ). The acidic eluate is concentrated and additionally dried under vacuum at 50 C.
Yield: 1.3 y (92.5%) Calculated: C 51.27 H 6.03 N 11.96 Found: C 51.19 H 5.99 N 11.91 iJ IJ ~1 ?.~
(c) Gadolinium Complex of 3,6,9-Tris(carboxymethyl)-3,6,9-triazaundecanedioic Acid Phenyl Monoamide ________________________________________________ 936 mg (2 mmol) of the complexing acid obtained according to Example llb) is dissolved in 5 about 40 mi of water and combined at 80 C with 362 mg (1 mmol) of Gd2O3. After 30 minutes, the almost clear solution is filtered off and the filtrate is freeze-dried.
Yield: 1.23 g (98.8%), based on anhydrous substance.
Calculated: C 38.S7 H 4.05 N 9.00 Gd 25.25 Found: C 38.33 H 4.10 N 9.03 Gd 24.99 Example 2 Preparation of a Solution of the N-Methylglucamine Salt of the Gadolinium Complex of 3,6,9-Tris(carboxymethyl)-3,6,9-triazaundecanedioic Acid Phenyl Monoamide _______________________________________________________ 1.87 g (3 mmol) of the gadolinium complex of 3,6,9-tris(carboxymethyl)-3,6,9-triazaundecanedioic acid phenyl monoamide (Example 1) is suspended in 5 ml of water pro injectione and combined with 0.586 g (3 mmol) of N-methylglucamine, thus dissolving the complex. The mixture is filled up with water to 10 ml, the solution is introduced into a vial and subjected to heat sterilization.
Tl relaxation (ltmmol sec) is:
25 in water: 3.79 + 0.16 in plasma: 5.45 + 0.68 ~xample 3 Preparation of a So]ution of the Sodium Salt of -the Gadolinium(III) Complex of 3,6,9-Tris(carboxymethyl)-3,6,9-triazaundecanedioic Acid Phenyl Monoamide _____________________________________________________ 62.27 g (0~1 mol) of the gadolinium complex obtained according to Example l(c) is suspended in 800 ml of water pro injectione (p.i.) and dissolved at pH 7.2 by dropwise addition of normal sodium hydroxide solution. After adding 0.2 g of tromethamine, the mixture is filled up with water p.i. to 1000 ml, the solution is dispensed into bottles and heat-sterilized.
Example 4 (a) 3,6,9-Tris(carboxymethyl)-3,6,9-triazaundecanedioic Acid (N,N'-Diphenyl)diamide ___________________________________________________ 42.88 g (120 mmol) of DTPA bis-anhydride is suspended in 330 ml of dimethylormamde and cooled in an ice bath under agitation to about 5 C. Within 50 minutes, a solution of 32.9 ml (360 mmol) of aniline in 30 ml of dimethylformamide is added dropwise. The mixture is stirred for another hour in an ice bath, then overnight at room temperature. ~ter this time, a slightly turbid solution has formed. The solvent is removed under vacuum, and the smeary residue is triturated with diethyl ether to remove traces of sol-vent. The residue is combined with 500 ml of water anddissolved by adding 20 ml of llN sodium hydroxide solution. The solution is combined with 3.5 g of active carbon, filtered, and freeze-dried, thus obtaining 73.9 g of the sodium salt of the title compound as a powder.
~ J~3~) ~
(b) Gadolinium Comple~ of 3,6,9-Tris~carboxymethyl)-3,6,g-triaZaUndeCanediOiC ACid IN,N~-DiPhenY1)-diamide __________________._____________________________ 10.3 g of gadolinium oxide (30 mmol) is heated under reflux with 10. 6 ml of glacial acetic acid and 150 ml of water for 20 minu-tes. The solution is filtered through a 0.1 ~m membrane filter, combined with 35 . 3 g (60 mmol) of the ligand obtained accord-ing to 4(a), and heated for 90 minutes to 80 C. The solution is stirred with 2.1 g of active carbon for 30 minutes, filtered., and then passed in succession over an anion exchange column (200 ml IRA-410) and - 100 ml of cation exchanger (IRC-50). The eluates from the columns a filtered through a 0.1 ~m membrane filter and freeze-dried, thus obtaining 17.6 g of the title compound as a white powder..
Analysis:
Calculated: C 44.75 H 4.33 Gd 22.54 N 10.04 Found: C 44.60 H 4.44 Gd 22.42 N 9.89 Example 5 (a) 3,6,9-Tris(carboxymethyl)-3,6,9-triazaundecanedioic Acid (N,N'-Dibenzyl)diamide ___________________ _________________.. ____________ 42.88 g (120 mmol~ of DTPA bis-anhydride is suspended in 330 ml of dimethylformamide and cooled in an ice bath under agitation to 5 C. Within one hour, a solution of 39.3 ml (360 mmol) of benzylamine in 30 ml of dimethylformamide is added dropwise. The mixture is stirred for another hour in the ice bath, then overnight at room temperature. After removal of the solvent under vacuum, the residue i5 triturated with diethyl ether, co~bined with 500 ml of water, and dis-solved by adding 20 ml of llN sodium hydroxide solution.
A~ter freeze-drying, 71 g of -the sodium salt of the title compound is obtained as a light-yellow powder.
(b) Gadolinium Complex of 3,6,9-Tris~carboxymethyl)-3,6,9-triazaundecanedioic Acid (N,N'-Dibenzyl)-diamide ________________________________________________ 10.9 g of gadolinium oxide (30 mmol) is heated under reflux with 10.6 ml of glacial acetic acid and 150 ml of water for 20 minutes. The solution is filtered o~er a 0.1 ~m membrane filter, combined with 37 g (60 mmol) of the ligand obtained according to 5(a), and heated for 90 minutes to 80~ C. The solution is stirred.with 3 g of active carbon for 30 minutes, filtered, and then passed in succession over an anion exchange column (200 ml IRA-410) and 100 ml cation exchange column (100 ml IRC-50). The eluates are filtered through a 0.1 ~m membrane filter and freeze-dried, thus obtaining 18 g of the title com-pound as a white powder.
Analysis:
Calculated: C 46.33 H 4.72 Gd 21.66 N 9.65 Found: C 46.46 H 4.49 Gd 21.50 N 9.81 f; '! f ~ ~I
~ w ~J
- 2~ -Example 6 (a) 6-Carboxymethyl-3-ethoxycarbonylmethyl-9-benzyl-aminocarbonylmethyl-3,6,9-triazaundecanedioic Acid __________________________________________________ 5.04 g (12.5 mmol) of N3-(2,6-dioxomorpholino-ethyl)-N~-(ethoxycarbonylmethyl)-3,6~diazaoctanedioic acid is suspended în 65 ml o~ dimeth~lformamide, stirred in an ice bath and combined, in succession, with 8.7 ml (62.5 mmol) of triethylamine and 1.34 g (12.5 mmol) of benzylamine. The mixture is then stirred for another 2 hours in the ice bath, then overni~ht at room temperature. The solvent is removed by vacuum distillation, the residue is agitated with diisopropyl ether, suctioned off, and dried. For further purification, the residue is dissolved in such a quantity of llN sodium hydroxide solution that a pH of 7 is just attained. To this mixture is added 5 g of silica gel, the suspension is dried under vacuum, and the residue is introduced into a column of 350 g of silica gel charged with a mixture of chloroform/meth-anol/glacial acetic acid/water (1750/1050/350/350).
The product is eluted with the same solvent and, after evaporation of the solvent, 4.98 g of a colorless, smeary substance is obtained which is dissolved in 35 ml of water and passed over a column with 35 ml of the cation e~changer"IR 120". The column is washed with 70 ml of water, the combined eluates are evap-orated under vacuum, and the residue is triturated with diethyl ether, thus obtaining 2.65 g of the title compound as a white powder.
Analysis:
Calculated: C 54.11 El 6.71 N 10.97 Found: C 53.95 H 6.88 N 11.23 ~ 47 ~
(b) 3,6,9-Tris(carboxymethyl)-3,6,9-triazaundecane-dioic Acid Benzyl Monoamide _____________._________________________________ A solution of 2.26 g of the compound prepared according to Example 6(a) in 46 ml of lN
sodium hydroxide solution is allowed to stand for 2.5 hours at room temperature, and the solution is then passed over a column of 110 ml of cation exchanger "IR 120". Elution is first carried out with 200 ml of water, and this fraction is discarded. Then, 600 ml of 0.5N ammonia is used for elution; this eluate is adjusted to pH 2.3 by adding "IR 120", and the solu-tion is subjected to freeze-drying, thus obtaining 1.50 g of the title compound as a white powder.
Analysis:
Calculated: C 52.28 H 6.27 N 11.61 Found: C 52.44 H 6.32 N 11.80 (c) Gadolinium Complex of 3,6,9-1'ris(carboxymethyl)-3,6,9-triazaundecanedioic Acid Benzyl Monoamide ______________ _________________________________ 965 mg (2 mmol) of the compound produced in accordance with Example 6(b) is heated with 40 ml of water and 362 mg (1 mmol) of gadolinium oxide for one hour to 80 C. The mixture is cooled to room tempera-ture, the solution is filtered through a 0.1 ~m membrane filter, and the compound is isolated by freeze-drying, yielding 1.15 g of the title compound as a white powder.
Analysis:
Calculated: C 39.61 H 4.27 Gd 24.70 N 8.80 Found: C 39.50 H 4.48 Gd 24.61 N 8.97 ~'J ~J '~
Example 7 Dysprosium(III) Complex of 3,6,9-Tris(carboxymethyl~-3,6,9-tria~.aundecanedioic Acid senzyl Monoamide _____________________________________________________ 965 mg (2 mmol) of 3,6,9-tris(carboxyme-thyl~-3,6,9-triazaundecanedioic acid benzyl monoamide (Example 6b) is heated with 40 ml of water and 373 mg (1 mmol) of dysprosium(III) oxide for one hour to 80 C.
The mixture is cooled to room temperature, the solution is filtered through a 0.1 ~m membrane filter, and the compound is isolated by freeze-drying, thus obtaining 1.22 g of the title compound as a white powder.
Analysis:
Calculated: C 39.29 H 4.24 Dy 25.31 N 8.73 Eound: C 39.41 H 4.51 Dy 25.19 N 8.70 Example 8 (a) 6-Carboxymethyl-3-ethoxycarbonylmethyl-9-tert-butylaminocarbonylmethyl-3,6,9-triazaundecane-dioic Acid ______________________________________________ At 0 C, 2.42 g (6 mmol) of N -(2r6-dioxo-morpholinoethyl)-N6-(ethoxycarbonylmethyl)-3,6-diaza-octanedioic acid is combined in DMF with 4.16 ml (3.04 g, 30 mmol) of triethylamine and 0.64 g (6 mmol) of tert-butylamine and stirred overnight at room tem-perature. The clear solution is then concentrated under vacuum and the residue chromatographed on silica gel with dichloromethane/methanol/acetic acid/water (5~3:1:1) as eluent. The combined fractions are passed over about 10 ml of "Amberlite" IR 120 (H form), and the acidic eluate is concentrated.
Yield: 2.14 g (75~) t~ 'J
Calculated: C 50.41 H 7.62 N 1l.76 Found: C 50.26 ~l 7.66 N 11.80 (b) 3,6,9-Tris(Carboxymethyl)-3,6,9--triazaundecane-dioic Acid tert-sutyl Monoamide __.____________________________________________ 1.43 g (3 mmol~ of the ethyl ester described in Example 8(a) is dissolved in 2N NaOH and stirred Eor 2 hours at room temperature. By addition of "Amberlite" 120 (H form), the mixture is adjusted to pH 7, filtered off, and the neutral solution passed over abou-t 16 ml of "Amberlite" IR 120 (H ). The acidic eluate is concentrated and further dried at 50 C
under vacuum. Yield: 1.20 g (89%).
Calculated: C 48.21 H 7.19 N 12.49 Found: C 48.13 H 7.24 N 12.41 (c) Gadolinium Complex of 3,6,9-Tris(carboxymethyl)-3,6,9-triazaundecanedioic Acid tert-Butyl Monoamide _________ ____ ______________ ________ ___ _ ______ 897 mg (2 mmol) of the complex-forming aeid obtained according to Example 8(b) is dissolved in about 40 ml of water and combined at 80 C with 362 mg (1 mmol) of Gd2O3. After 30 minutes, the almost elear solution is filtered and the filtrate freeze-dried.
Yield: 1.19 g (99%), based on anhydrous substanee.
Calculated: C 35.87 H 4.85 N 9.30 Gd 26.09 Found: C 35.97 H 4.79 N 9.28 Gd 25.83 f~
- 2~ -Example 9 (a) 6-Carboxymethyl-3-ethoxycarbonylmethyl-9-(4-methoxyben~ylcarbamoylmethyl)-3,6,9-triazaundecanedioic Acid ______________________________ 14.10 g (35 mmol) of N -(2,6-dioxomorpholino-ethyl)-N6~(ethoxycarbonylmethyl)-3,6-diazaoctanedloic acid is combined in 175 ml of dimethylformamide at 0 C with 24.4 ml (175 mmol) of triethylamine and 4.67 ml (35 mmol) of 4-methoxybenzylamine and stirred overnight at room temperature. The solvent is extensively removed by vacuum distillation and the residue is heated to boiling with 600 ml of diisopropyl ether. After cool-ing to room temperature, the mixture is decanted off from the solvent. The residue is dissolved in 185 ml of water and passed-over a column with 140 ml of cation exchanger "IR 120" (H+ form), and the column is washed with 200 ml of water. The combined eluates are con-centrated to one-third under vacuum and then freeze-dried, yielding 16.33 g of the title compound as a white powder which still contains 1.2~ water and 1~ dimethyl-formamide.
Analysis tafter correction of solvent proportions):
Calculated: C 53.33 H 6.71 N 10.36 Found: C 53.51 H 6.78 N 10.18 .
- - ~
(b) 3,6-Bis(carboxymethyl~-9-(4-methoxybenzylcarbamoyl-methyl)-3,6,9-triazaundecanedioic Acid ___________~_______________~_______________ _______ 12.34 g (22 mmol) of the ethyl ester described in Example 9(a3 is dissolved in 200 ml of water and 20 ml of llN sodium hydroxide solution and stirred for 2 hours at room temperature. A pH of 2.3 is reached by addition of 140 ml of "Amberlite" IR 120 (H form). The mixture is filtered and the solution subjected to freeze-drying.
10 Yield: 9.61 g (85% of theory?, water content 2.18~.
Analysis (after correction of water content):
Calculated: C 51.56 H 6.29 N 10.93 Found: C 51.37 H 6.44 N 10.89 (c) Gadolinium Complex of 3,6-Bis(carboxymethyl)-9-(4-methoxybenzylcarbamoylmethyl)-3,6,9-triazaundecanedioic Acid _______________________________________________ 5.24 g (10 mmol) of the complexing acid obtained according to Example 9(b) is stirred in 200 ml of water with 1.81 g ~5 mmol? of gadolinium oxide for one hour at ~0 C, producing an almost clear solution.
The latter is filtered and the filtrate subjected to freeze-dryin~.
Yield: 6.31 g, water content 3.7%.
Analysis (after correction of water content):
25 Calculated: C 39.63 H 4.38 N 8.40 Gd 23.99 Found: C 39.88 H 4.53 N 8.51 Gd 23.70 s ~
(d) N-Methylglucamine Salt of the Gadolinium Complex of 3,6-Bis(carboxymethyl~-9-(4-methoxybenzyl-carbamoylmethyl~-3,6,9-triazaundecanedioic Acid ________________________________________________ 2 g of the gadolinium complex obtained according to Example 9(c) is dissolved in 30 ml of waterl combined with 1 equivalent of N-methylglucamine, and the solution is concentrated by evaporation under vacuum.
Yield: 2.30 g, water content 4%.
Analysis (after correction of water content):
Calculated: C 40.41 H 5.38 N 8.12 Gd 18.24 Found: C 40.52 H 5.11 N 8.27 Gd 18.39 (e) Sodium Sal-t of the Gadolinium Complex of 3,6-Bis(carboxymethyl)-~-(4-methoxybenzyl-carbamoylmethyl)-3,6,9-triazaundecanedioic Acid ____________________________________________ __ 0.5 g of the gadolinium complex obtained in accordance with Example 9(c) is dissolved in lO ml o~
water, combined with l equivalent of sodium~hydroxide dissolved in 5 ml of water, and the solution of the title compound is subjected to freeze-drying, producing 0.55 g of the title compound as a white powder with a water content of 4.5~.
Analysis (after correction of water content):
Calculated: C 38.37 H 4.10 Gd 22.83 N 8.13 Na 3.34 Found: C 38.40 H 4.45 Gd 22.43 N 8.10 Na 3.57 - . ~
~ ^ I ~ ' . ' ! ' Example 10 (a) 6-Carboxymethyl-3-ethoxycarbonylmethyl-9-(N-undecylcarbamoylmethyl)-3,6,9-triaza-undecanedioic Acid _________________________________________ Under a nitrogen atmospherel 12.10 g (30 mmol) of N3-(2,6-dioxomorpholinoethyl)-N6-(ethoxycarbonylmethyl)-3,6-diazaoctanedioic acid in 1 liter of absolute dimethylformamide is combined at 0 C with 12.6 ml (91 mmol) of triethylamine and 5.14 g (30 mmol) of l-undecylamine and stirred for 16 hours at 20-25 C. After the reaction is completed, the solvent is evaporated under vacuum and the remain-ing oily residue is stirred with 1 liter of diethyl ether. The thus-separated white powder is suctioned off, rinsed with 1 liter of diethyl ether in portions, and the product is dried at 40 C under vacuum.
Yield: 14.31 g (83%~, white powder.
Water content: 1.41 Dimethylformamide content: 0.8~
Analysis (after correction of solvent proportions):
Calculated: C 56.43 H 8.77 N 9.75 Found: C 56.25 H 8.89 N 9.48 (b) 3,6-Bis(carboxyme-thyl)-9-(N-undecylcarbamoyl-methyl)-3,6,9-triazaundecanedioic Acid __________________________________________ __ 10 g (17.4 mmol) of the ethyl ester described in Example lO~a) is dissolved in 200 ml of 2N sodium hydroxide solution and stirred for 2 hours at room temperature. After the reaction is finished, the mixture is cooled to 5 C and concentrated hydro-chloric acid is added until a pH value of 2.15 has been ~J ~ C~
attained. The thus-separated white powder i5 suctioned off and washed five times with 50 ml of ice water, five times with diethyl ether/ethanol (8:2), five times with S0 ml of diethyl ether, and five times with 50 ml of n-pentane.
Yield: 8.25 g t86.7~, white powder.
Water content: 2.12%
Dimethylformamide content: < 0.05%
Analysis (after correction of water content):
10 Calculated: C 54.93 H 8.48 N 10.25 Found: C 54.78 H 8.67 N 9.98 (c) Gadolinium Complex of 3,6-Bis(carboxymethyl)-9-(N-undecylcarbamoylmethyl)-3,6,9-tria~aundecane-dioic Acid __________________ _____________________________ At 80 C, 5.47 g (10 mmol) of the complex-forming acid obtained according to Example lO(b) is stirred in 500 ml of water with 1.81 g (5 mmol) of gadolinium oxide for 4 hours, thus producing an almost clear solution. The latter is filtered and the filtrate subjected to free~e-drying.
Yield: 6.35 g (90.6%), white powder.
Water content: 4.2%
Analysis (after correction of water content):
Calculated: C 42.84 ~1 6.18 N 7.99 Gd 22.44 25 Found: C 42.91 H 6.25 N 7.87 Gd 22.40 (d) Mono-N-methylglucamine Sal-t of the Gadolinium Complex of 3,6-Bis(carboxymethyl)-9-(N-undecyl-carbamoylmethyl)-3,6,9-triazaundecanedioic Acid 2 g of the gadolinium complex obtained in accordance with Example lO(c) is dissolved in 35 ml of water, combined with one equivalent of N-methyl-glucamine, filtered, and the solution is evaporated under vacuum.
Yield: 2.25 g (88%), white powder.
Water content: 3.75%
Analysis (after correction of water content): ~
Calculated: C 42.89 H 6.75 N 7.82 Gd 17.55 Found: C 42.73 H 6.89 N 7.69 Gd 17.48 Example 11 5 (a) 6-Carboxymethyl-3-ethoxycarbonylmethyl-9-(1-hexa-decylcarbamoylmethyl)-3,6,9-triazaundecanedioic ~cid 28.20 g (70 mmol) of N3-(2,6-dioxomorpholino-ethyl)-N6-(ethoxycarbonylmethyl)-3,6-diazaoctanedioic acid is combined in 320 ml of dimethylformamide at 0 C wi-th 28.8 ml (350 mmol) of triethylamine and 16.90 g (70 mmol) of 1-hexadecylamine and then stirred for 24 hours at 20-25 C. The mixture is therea~ter concentrated under vacuum and the residue stirred under boiling heat with 1 liter of methyl tert-butyl ether.
After cooling to +10 C, the mixture is suctioned off, the residue dried at 45 C under vacuum, taken up in 400 ml of water, and the solution is passed over a column with 300 ml of ion e~changer "IR 120" (H Porm), the column is washed with 0.5 liter of water, and the combined eluates are concentrated under vacuum to about - 3~ -300 ml, and the title compound is isolated by freeze-dryingl thus obtaining 36.5 g as a white powder.
Water content: 1.70%
Dimethylformamide conten-t: 0.7%
Analysis (after correction of solvent proportions):
Calculated: C 59.60 ll 9.38 N 8.69 Found: C 59.42 H 9.49 N 8.85 (b) 3,6-Bis(carboxymethyl)-9-(1-hexadecylcarbamoyl-methyl)-3,6,9-triazaundecanedioic Acid _______________________________________________ 6.45 g (10 mmol) of the ethyl ester disclosed in Example ll(a) is dissolved in 100 ml of water and 9.1 ml of llN sodium hydroxide solution and left for 2 hours at room temperature. Under agitation, the mix-ture is combined with 65 ml of ion exchanger "Amberlite"
IR 120 (H form), thus setting a pH of 2.5. The mix-ture is filtered and the solution is subjected to freeze-drying, thus obtaining 5.30 g of the title compound as a white powder.
Water content: 3.2%
Dimethylformamide content: ~ 0.05~
Analysis (after correction of water content):
Calculated: C 58.42 El 9.15 N 9.08 Found: C 58.59 H 9.44 N 8.95 ~c) Monomeglumine Salt of -the Gadolinium Complex of 3,6-sis(carboxymethyl~-9-(1-hexadecylcarbamoyl-methyl)-~,6,9-triazaundecanedioic Ac.id _______________________________________________ A-t 80-85 C, 4.93 g (8 mmol) of the complex-Eorming acid obtained according to Example ll(b~
is stirred in 170 ml of water for 2 hours with 1.45 g (4 mmol) of gadolinium oxide and 1.56 g (8 mmol) of N-methylglucamine. The almost clear solution is filtered and Ereeze-dried.
~ield: 7.49 g of a white powder.
Water content: 2.8~
Analysis (after correction of water content):
Calculated: C 45.99 H 7.30 N 7.25 Gd 16.27 Found: C 46.21 H 7.55 N 7.08 Gd 16.18 By the same route as described in Example 11, the monomeglumine salt of the europium complex of 3,6-bis(carboxymethyl)-9-(1-hexadecylcarbamoylmethyl3-3,6,9-triazaundecanedioic acid can be prepared as well.
Example 12 Preparation of a Solution of the Meglumine Salt of the Gadolinium Complex of 3,6,9-Tris(carboxymethyl)-3,6,9-tri.azaundecanedioic Acid Undecyl Monoamide _____________________________________________________._ 448.57 g (0.5 mol) of the compound described in Example 10 ~d) is dissolved under heating in 600 ml o~
water pro injectione (p.i.). After addition of 4.92 g (10 mmol) of the monohydrate of the calciumtrisodium salt of DTPA, CaNa3DTPA, tlle solution is filled up with water p.i. to 1000 ml. The solution is subjected to ultrafiltration, dispensed into bo-tt].es, and heat-sterilized, and is ready ~or use :Eor parenteral admini-stration.
C 1 ' " ! ' ' Example 13 Production of a Powder Form of Administration 89.61 g (0.1 mol) o~ the meglumine salt disclosed in Example lO(d) is finely ground up with 25 g of sucrose and 5 g of "Pluronic" F 68 and lo mg of raspberry flavoring. The powder is filled into bags and is ready for oral administration.
The preceding examples can be repeated with similar success by substituting the generically or specifically described reactants and/or operating conditions of this invention for those used in the preceding examples.
From the foregoing description, one skilled in the art can easily ascertain the essential ch~racteristics of this invention, and without departing from the spirit and scope thereof, can make various changes and modifications of the invention to adapt it to various usages and conditions.
US~ OF AMIDE COMPLEX COMPO~DS
Cross-Reference to Related Ap~lications This application is a continuation-in-part of Serial No. 07/495,803, filed March 14, 1990, which is a 5 continuation of Serial No. 07/100,6~, filed September 24, 1987 (abandoned), both of which are entirely incorporated by reference herein.
In addition, this application is related to U.S.
Application Serial Nos. 07/078,~07 (filed July 28, 1987), 07/063,355 (filed June 18, 1987), 07/020,301 (filed March 2, 1987), 07/020,300 (filed ~arch 2, 1987), 07/020,993 (filed March 2, 19~7), 07/020,992 (filed March 2, 1987), 06/936,055 (filed November 28, 1986), 06/876,497 (filed June 20, 1986), and 06~627,143 (filed July 2, 1984), each of which is a divisional, continuation or C-I-P of - 06/573,184 (filed January 23, 1984), now U.S. Patent No.
4,647,447, which is a C-I-P of 06/401,594 (filed July 26, 1982), and this application is a C-I-P of all of said applications directly or indirectly and all of which applications are entirely incorporated by reference herein.
Summary of the Invention This invention relates to physiologically compatible amide complex compounds and the use there~f in the production of agents for organ-specific N~R diagnostics and for cases involving patients with ren~l insufficiency, as well as NMR msthods utilizing these compounds.
European Patent Application Publication Number 263,059 claims compounds of Formula I R3 Y--CH2 CH2CO--N~ 4 N - -- CH--(CH2~1 -CH2) n CIH I (I) XOOCCH2 R CHzCOOX R CH2COOX
wherein nis o, 1 or 2, 10 Rl and R2 independently are hydrogen atoms, lower alkyl groups, phenyl groups, benzyl groups or, if n is O, jointly can also form a trimethylene or a tetramethylene group, R3 is a saturated, unsaturated, straight-chain or branched-chain or cyclic aliphatic hydrocarbon residue of up to 16 carbon atoms and, if R4 is a hydrogen atom, at least one R3 :i.s a cycloalkyl group, or an aryl or aralkyl group optionally substituted by one or several di C1-C6-alkylamino groups or by one or several C1-C6-alkoxy groups, R4 is a hydrogen atom, or a saturated, unsaturated, straight-chain or branched-chain or cyclic hydrocarbon residue of up to 16 carbon atoms, or R3 and R4 jointly form a satuxated or uns~turated 5- or 6-membered ring which is option~lly substituted by one or several of C1-C6-alkyl, C1-C5-hydroxy-alkyl, optionally hydroxylated ~r C1-C6-alkoxy-lated Cz-C6-acyl, hydroxy, carbamoyl, carbamoyl-substituted C1-C6 alkyl residue, carbamoyl substituted on the carhamoyl nitrogen by one or two C1-C6-alkyl residue(s) -- which can also form a ring optionally containing an oxy-gen atom -- or C1-C6-acylamino or C1-C6-alkyl-amino;
this 5- or 6-membered ring opti~nally containing a furkher nitrogen, ~xygen or sulfur atom, or carbonyl group, X means a hydrogen atom and/or a metal ion equivalent, R 3 Y is a COOX or ~ON\ group, as well as their salts with organic and/or inorganic bases.
Compounds havirlg the anion of one oF
these complex-forming amides and one or several central ions of an element of atomic numbers 21-29, 31, 32, 38, 39, 42-44, 49, 57-83 and optionally one or several cations of an inorganic and/or organic base or amino acid are suited for the production of NMR, X-ray and radiology diagnostic media.
It has now been found that surprisingly an unexpected pharmacokinetic behavior is displayed by compounds of this general Formula I if these compounds contain at least one element of atomic numbers 21-29, 42, 44 or 58-70, i.e.,at least two of the substituents X must stand for a metal ion equivalent of these ele-ments, but in particular compounds of general Formula I wherein n is the number 1, R a n d R are hydrogen atoms, R3 is a saturated, unsaturated, straight-chain or branched-chain or cyclic aliphatic hydrocarbon residue of u~ to 16 carbon atoms and, if R is a hydrogen atom, at least one R3 is a cycloalkyl group, or an aryl or aralkyl group optionally substituted by one or several di-C1-C6-alkylamino groups or by one or several C1-C6-alkoxy groups, R4 is a hydrogen atom or a saturated, unsaturated, straight-chain, branched-chain or cyclic hydrocarbon residue of up to 16 carbon atoms, X is a hydrogen atom and/or a metal ion equivalent of at least one element of atomic numbers 21-29, 42, 44 or 58-70, R
/
Y is a COOX or CON~ group, R
with the proviso that at least two of the substituents X stand for a metal ion equivalent, as well as their salts with organic and/or inorganic bases.
Thus, for example, MAGNEVIST ~, thus far the only NMR contrast medium permitted worldwide, is distributed upon intravenous injection in an extra-cellular fashion and is excreted via the kidneysby glomerular secretion. Passage of intact cell membranes and extrarenal excretion are practically not at all observed.
MAGNEVIST ~ is especially well suited for the diagnosis of pathological regions (for example, inflammations, tumors, infarctions, etc.).
Contrast media exhibiting an at least partial extrarenal excretion would be desirable, especially for patients with limited kidney function (renal insufficiency) where MAGNEVIST G~is excreted only very slowly and, in part, can be removed from the organism only with the aid of a dialysis device.
r~ J ~ J
Consequently, there is a need ~or NMR contrast media e~hibiting a di~ferent pharmacokinetic behavior and thus higher organ specificity than MAGNEVIST~.
Accordingly, it is an object o~ this invention to provide such compounds and media and methods of using same.
Upon further study of the specification and appended claims, further objects and advantages of this invention will become apparent to those skilled in the art.
It has been found that the above-mentioned compounds surprisingly show the desired property:
renal elimination as well as excretion with the feces.
Surprisingly, elimination via the gallbladder, however, is not the only extrarenal path of elimination:
in NMR studies on rats, upon intravenous administration of the compounds of this invention, a contrast enhance-ment of the gastrointestinal tract has also been unexpectedly observed. The kidneys, as well as implanted tumors, are likewise visualized with improved contrast.
Elimination ~secretion) by way of the stomach ~` has the advantage that contras-ting of abdominal struc-tures (e.g. pancreas) from the gastrointestinal tract is made possible, with a simultaneous contrast enhance-ment of pathological processes (tumors, inflammations).
Imaging of the renal system, of the liver and gallbladder, and the bile ducts can moreover likewise be achieved.
Besides the improved visualization of ulcers and stomach carcinomas, it is also possible to perform studies on gastric acid secretion with the aid of imaging procedures.
Accordingly, by making the compounds of this invention available, help can be e~tended to patients with renal insufficiency as well as patients suffering from gastrointestinal disorders (at least 10% of the population in the Western industrial countries).
patients suspected of harboring such disease, must submit to diagnos-tic tests. At present, two methods suitable fo~ this purpose are utilized above all:
endoscopy and X-ray diagnos-tics with the aid of barium contrast media.
These tests exhibit various drawbacks: they carry the risk of radiation stress, cause trauma, are connected with inconveniences, occasionally even with risks for the patient, and thus can evoke psychological stress. In most cases, these tests must be repeated;
their performance is relatively complicated, require the patient's active cooperation (e.g. assumption of a specific bodily attitude) and frequently cannot be employed in case of frail and high-risk patients.
The object of providing novel diagnostic methods for the identification and localization of gastrointestinal diseases, which methods do not exhibit these drawbacks, has thus likewise been attained by the complex compounds and agents as mentioned above.
Their pharmacokinetics permit, even wi-thout specific measures, an improvement in the diagnosis of numerous diseases. The complexes for the most part are excreted again in unchanged form and rapidly so that, especially also in case of using relatively toxic metallic ions, no damaging effects are observed even at high dosage.
The practical use of the novel ccmplexes is also facilitated by their favorable chemical stability.
~-" f ~ r ," ~
Compounds of formula I, wherein X is hydrogen, are called "complexing agents", and those wherein at least two of the substituents X are a met~l ion equivalent are called "metal complexes~'.
For use in NMR diagnostics, the central ion of the complex salt will be paramagnetic. These are, in particular, the divalent and trivalent ions of the elements of atomic numbers 21-29, 42, ~4 and 58-70.
Suitable ions include, for example, the chromium(III), manganese(II), iron(II), cobalt(II), nickel(II), copper(II), praseodymium(III), neodymium(III), samarium(III) and ytterbium(III) ions. On account of their very strong magnetic moment, the gadolinium(III~, terbium(III), dysprosium(III), holmium(III); erbium(III) and iron(III) ions are espscially preferrad.
Suitable alkyl substituents R1 and R2 are hydrocarbons of 1-8, preferably 1-4 carbon atoms, such as, for example, methyl, ethyl, propyl, isopropyl, n-, sec- or tert.-butyl, isobutyl, and all is~mers of pentyl, hexyl, heptyl and octyl.
Suitable aliphatic substituents R3 and R4 are saturated (e.g., alkyl), unsaturated (e.g., alkenyl), straight-chain or branched-chain or cycli- hydrocarbons of up to 16 carbon atoms, pre~erably 1-10 C atoms, most preferably saturated hydrocarbons of 1-10 carbon atoms, especially saturated hydrocarbons of 1-5 -arbon atoms.
Examples include methyl, ethyl, propyl, i~opropyl, butyl, isobutyl, pentyl, cyclopentyl, cyclohexyl, propenyl, etc.
Other suitable groups are the other alkyl groups mentioned above for R1 and R2 and isomers containing 9-16 C atoms as well as their ~lkenyl counterparts.
When R4 is a hydrogen atom, at :east one R3 is preferably C6-C10-aryl or C6-C10-Ar-C1-C6-alcyl group, e.g., phenyl or benzyl group, optionally substituted by one or several ~e.g., up to three) di-C1- to C6-alkylamino groups , or by one or several (e.g., up to three) C1- to C6-alkoxy groups.
In addition, when ~' i6 a hydrogen atom, R3 can also preferably be a cycloalkyl group, as mentioned above. The cycloalkyl group generally contains 3-16 carbon atoms, preferably ~-7 carbon atoms.
The heterocyclic 5- or 6-membered ring formed by R3 and R4 with inclusion of the amide nitrogen can be saturated, unsaturated andjor substituted and can optionally contain a nitrogen, oxygen or sulfur atom or carbonyl group.
The heterocycle can be substituted by hydroxy, C1-C6-alkyl, e.g., methyl, ethyl, propyl, isopropyl, butyl, C1-C5-hydroxyalkyl, e.g., hydroxymethyl, hydroxyethyl, or by C2-C6-acyl (e.g., alkanoyl), for example acetyl, propionyl, which can, if desired, be substituted by hydroxy or C1-C6-alkoxy, e.g., methoxy, ethoxy, etc.
A further substituent that can be mentioned is carbamoyl, linked to the heterocycle dire-tly or separaked by a C1-C6-alkylene group, for example methylene, ethylene, propylene, and whicn can also be substituted at the nitrogen, if desired, by one or two C1-C6-alkyl residue(s), e.g., methyl, ethyl, propyl, isopropyl, ~tc. The alkyl groups can, optionally, form a ring, such as, for example, a pyrrolidine or piperidine ring. The carbamoyl nitrogen can also be part of a morpholine ring, i.e., the latter ring can have an 0 atom.
Another possible substituent on the heterocycle that can be mentioned is an optionally C1-C6-alkylated or C1-C6-acylated (e.g., alkanoylated~ primary or secondary amino group, such as, for example, the methyl-, ethyl-, acetyl-, propionyl-, amino-, etc., group.
If the heterocycle is substituted, the total number of substituents is l to 3.
t ~
Suitable heterocycles are, for example: the pyrrolidinyl, piperidyl, pyrazolidinyl; pyrrolinyl, pyrazolinyl, piperazinyl, morpholinyl, imidazolidinyl, oxazolidinyl, thiazolidinyl rings.
If not all of the acidic hydrogen atoms are substituted by the central ion, then one, several, or all remaining hydrogen atom(s) can be replace~ by cations of inorganic and/or organic bases or amino acids. Suitable inorganic cations include, for example, the lithium ion, the potassium ion, the calcium ion and, in particular, the sodium ion. Suitable cations of organic bases include, inter alia, those of primary, secondary or tertiary amines, e.g., ethanolamine, diethanolamine, morpholine, glucamine, N,N-dimethylglucamine and especially N-methylglucamine. Suitable c~tions of amino acids include, for example, those of lysine, of arginine, and of ornithine.
Introduction of amide groups for the production of the complexing agents, i.e., of compounds of general Formula I wherein X means hydrogen, takes place by conventional partial conversion of activated carboxyl groups into amide groups of the respectively suited - tetra-, penta- and hexacarboxylic acids -- in correspondence with the desired final pro~uct. All of the synthesis pathways known to a person skilled in the art are suitable for this procedure.
One example is the reaction of -the anhydrides or esters of gener~l E~ormulae II, IV, V and VI:
VOC-C~ ~CH2-COV
N-Cj H- ( CH2- 1 -CH2 ) n-CI H-rl \ ( II ) ZOC-CH2 Rl CH2COOH R2 C~z-COZ
HOOC-C~ ~CH2-CO~l / N-CH- ( CH2-N-CH2 ) -CH-N \ ( IV ) R500C-CH2 Rl CH2COOH R CH -COZ
HOOC-CH2 ~ CH2COOH
~ N-CH-(CH2-N-CH2)n-~H-N ~ (V) R500C-CH2 Rl CH2COOH ~2 CH2COOH
HQQC- CH `
.j / \
N -CH - t CH2-N -CH2 ) n-CH -N ( V I ) .
R OOC-CH2 ¦1 CH2COOH R ~~
wherein Rl, R2 and n have the above-mentioned meanings, V and Z jointly mean an oxygen atom, or V is a hydroxy group and Z is the group.ing oR5~ wherein R is a C1~C6~a1ky1 residue, hs ~.3 .'; C.J ~ J
with amines oE general Formula III
HN ~ I I I ), wherein ~3 and R4 have the meanings given above.
Examples of suitable amines include: dimethyl-amine, diethylamine, di-n-propylamine, diisopropylamine, di-n-butylamine, diisobutylamine, di-sec-butylamine, N-methyl-n-propylamine, dioctylamine, dicyclohexyl-amine, N-ethylcyclohexylamine, diisopropenylamine, benzylamine, aniline, 4-methoxyaniline, 4-dimethyl-aminoaniline, 3,5-dimethoxyaniline, 4-methoxybenzyl-amine, morpholine, pyrrolidine, piperidine, N-methyl-piperazine, N-ethylpiperazine, N-(2-hydroxyethyl~-piperazine, N-(hydroxymethyl)piperazine, piperazino-acetic acid isopropylamide, N-(piperazinomethyl-carbonyl)morpholine, N-(piperazinomethylcarbonyl)pyr-rolidine, 2-(2-hydroxymethyl)piperidine, 4-(2-hydroxy-ethyl)piperidine 7 2-hydroxymethylpiperidine, 4-hydroxymethylpiperidine, 2-hydroxymethylpyrrolidine, 3-hydroxypiperidine r 4-hydroxypiperidine, 3-hydroxy-pyrrolidine, 4-piperidone, 3-pyrroline, piperidine-3-carboxylic acid amide, piperidine-4-carboxylic acid amide, piperidine-3-carboxylic acid diethylamide, piperidine-4-carboxylic acid dimethylamide, 2,6-dimethylpiperidine, 2,6-dimethylmorpholine, N-acetyl-piperazine, N-(2-hydroxypropionyl)piperazine, N-(3-hydroxypropionyl)piperazine, N-(methoxyacetyl)-piperazine, 4-(N-acetyl-N-methylamino)piperidine, -;
~Z~ u ~-1 ,J ~.., ,~
piperidine-4-carboxylic acid (3-oxapentamethylene)amide, piperidine-3-carboxylic acid 13-oxapentamethylene)amide, N-~N',N'-dimethylcarbamoyl3piperazine, pyrazoline, pyrazolidine, imidazoline, oxazolidine, thiazolidine, etc.
The saponification of any ester groups that may still be present takes place according to methods known to one skilled in ~he art, for example,by alkaline hydrolysis.
The acid anhydrides of general Formula II
can be prepared conventionally, for example in accord-ance with the mode of operation disclosed in U.S.
Patent 3,660,388 or DOS 1,695,050, with acetic anhydride in pyridine. However, in certain instances,it is especially advantageous to conduct the lS step of splitting off water in a gentle fashion with carbodiimides in a suitable solvent, such as, for ex-ample dimethylformamide or dimethylacetamide.
The preparation of the monoanhydrides of gen-eral Formula VI will be described by using as the example the monoanhydride of diethylenetriaminepenta-acetic acid ethyl ester starting with the monoethyl ester of DTPA (J. Pharm. 5ci. 68 : 194, 1979):
N3-(2,6-Dioxomorpholinoethyl)-N6-(ethoxycarbonyl-methyl)-3,6-diazaoctanedioic Acid _________________________________________________ A suspension of 21.l g (50 millimoles) of N3,N6-bis(carboxymethyl)-N9~(ethoxycarbonylmethyl)-3,6,9-triazaundecanedioic acid in 250 ml of acetic anhydride is agitated for 3 days at room temperature after adding 42.2 ml of pyridine. Then the precip-itate is suctioned off, washed three times with re-spectively 50 ml of acetic anhydride and subsequently ~,J i~ ~.,J ~ ~, 3 ~1 - 13 ~
stirred for several hours with absolute diethyl ether.
Af-ter suctioning off the product, washing same with absolute diethyl e~her and drying under vacuum at 40 C, 18.0 g (= 89% of theory) of a white powder is obtained, mp 195-196 C.
Analysis (based on anhydrous substance):
Calculated: C 47.64 ll 6.25 N 10.42 Found: C 47.54 El 6.30 N 10.22 The reaction of the acid anhydrides to the amides can be performed in the liquid phase. Suitable reaction media include, for example, water, dipolar aprotic solvents, such as acetonitrile, N-methylpyrrolidone, dimethylformamide, dimethylacetamide, and the like, or mixtures thereof. The reaction temperatures range between about 0 C and 100 C, temperatures of ~0-80 C being preferred. The reaction periods xange between 0.5 hour and 2 days, preferably between 1 hour and 36 hours.
The esters of general Formula V are produced conventionally, for example according to the processes described in R.A. Guilmette et al., J. Pharm~ Sci.
68 : 194 (1979).
Aminolysis of the esters takes place in the liquid phase, for example in a suitable higher-boiling solvent, such as dimethylformamide, dimethylacetamide or dimethyl sulfoxide. The reaction temperatures are around 20 C to 200 C~ temperatures of 100-180 C
being preferred. The reaction times range between 2 hours and 2 days, reaction periods of between 4 houxs and 36 hours being the preferred ones.
~ J~3~
1 'I -Moreover, all methods known to a person skilled in the art for converting carboxyl groups into amide groups can be employed for the synthesis of the complex-ing agents of Formula I according to this invention, For example, the method by Krejcarek and Tuc~er, Biochm. Biophys. Res. Comrnun. 77 : 581 (1977) via mixed anhydrides.
The resultant compounds of Formula I
wherein X is a hydrogen atom represent complex-forming media. They can be isolated and purified, or they can be converted without isolation into metal complexes of general Formula I wherein at least two of the substi-tuent X mean a metal ion equivalent.
The metal complexes are prepared conven~ionally by the methods disclosed in Patents EP 71564, EP 130934 and DOS
3,401,052,by dissolving or suspending the metal oxide or a metal salt (e.g.,the nitrate, acetate, carbonate, chloride or sulfate) of the element of atomic numbers 21-Z9, 42, 44 or 58-70 in water and/or a lower alcohol (such as methanol, ethanol or isopropanol) and reacting with a solution or suspension of the equivalent amount of the complex-forming acid of Formula I wherein X means a hydrogen atom and subsequently, if desired, substituting any acidic hydrogen atoms of acid groups present by cations of inorganic and/or organic bases or amino acids.
Neutralization is herein effected with the air of inorganic bases (for example, hydroxides, carbonates or bicarbonates) of, for example, sodium potassium, lithium and/or organic bases such as, inter alia, primary, secondary and tertiary amines, such as, for example, ethanolamine, morpholine, glucamine, N-methyl- and N,N-dimethylglucami~e, as well as basic amino acids, such as, for example, lysine, arginine and ornithine.
f~J ~ 2 s~
In order to prepare the neutral complex com-pounds, it is possible, for example, to add to the acidic complex salts in an aqueous solution or suspen-sion such an amount of the desired bases that the neutral point is reached. The resultant solution can subsequently be evaporated to dryness under vacuum.
It is frequently advantageous to precipitate the thus-formed neutral salts by adding water-miscible solvents, such as, for example, lower alcohols (methanol, e-thanol, isopropanol, etc.), lower ketones (acetone etc.), polar ethers ~tetrahydrofuran, dioxane, 1,2-dimethoxy-ethane, etc.), and to obtain in this way crystallized products which can be readily isolated and easily purified. It has proven to be especially advantageous t~
add the desired base to the reaction mixture as early as during the complexing reaction, thereby saving a process step.
If the acidic complex compounds contain several free acidic groups, then it is frequently ex-pedient to prepare neutral mixed salts containinginorganic as well as organic cations as the counterions.
This can be done, for example, by reacting the complexing acid in an aqueous suspension or solu-tion with the oxide or salt of the element yieldin~
the central ion and with half the amount of an organic base needed for neutralization, isolating the thus-formed complex salt, purifying same if desired, and then combining same for complete neutralization with the required amount of inorganic base. The sequence of adding the bases can also be reversed.
The diagnostic media are likewise produced in a manner known per se by suspending or dissolving the complex compounds of this invention -- optionally after adding the additives customary in galenic pharmacy -- in an aqueous medium and subsequently , . ' " ,~`. !,; ` C " ' ~
sterilizing the suspension or solution, if desired.
Suitable additives are, for example, physiologically acceptable buffers (such as, e.g., trome-thamine), small additions of complexing agents (such as, e.g., diethylene-triaminepentaacetic acidj or, if necessary, electrolytessuch as, for example, sodium chloride or, if needed antioxidants, such as ascorbic acid, for example.
If, for enteral administration or other purposes, suspensions or solutions of the media of this invention in water or a physiological saline solu-tion are desirable, they can be mixed with one or several auxiliary agents customary in galenic pharmacy (for example methylcellulose, lactose, mannitol) and/or tensides (e.g., lecithins, TWEENS(~), MYRJ(~).and/or lS flavoring substances for taste improvement (e.g.
ethereal oils).
In principle, it is also possible to prepare the diagnostic media of this invention even without isolation of the complex salts. In any event, special care must be directed toward effecting the chelate formation so that the salts and salt solutions accord-ing to this invention are practically devoid of toxically active metal ions that are not complexed.
This can be ensured, for example, with the aid of color indicators, such as xylenol orange, by control titrations during the manufacturing process.
Consequently, the invention also relates to processes for preparing the complex compounds and their salts.
The final safety feature resides in purification of the isolated complex salt.
For nuclear spin tomography diagnostics in accord-ance with this invention, the diagnostic media are ad-ministered in a dosage of 1 ~mol/kg to 5 mmol/kg, prefer-ably 10 ~mol to 0.5 mmol/kg of the complex sa3t according to the invention. rn case of intravenous injection, aque-ous formulations are ussd with a concentr~tion of 50 ~mol/l to 2 mol/l, preferably 100 mmol/l to 1 mol/l. Rec-tal as well as oral administration is preferably per-formed with solutions of a concentration ~f 0.1 mmol to 100 mmol/l. The volumes administered ran~e from about 5 ml to 2 1, in dependence on the diagnostic problem.
Thus, the diagnostic media are intended for enteral and parenteral administration to mammals, including humans.
The diagnostic media fulfill thQ variegated requirements for suitability as contrast media for nuclear spin tomography. Thus, they are ~xcellently suited, upon oral or parenteral administration, for improving the information content of the image obtained with the aid of the nuclear spin tomograph, by increasing the signal intensity. They show furthermore the high efficacy necessary for burdening the body with minimal amounts of foreign substances, and the go3d compatibility required for maintaining the noninvasive -haracter of the tests.
The high water solubility of the diagnostic media permits production of highly concentrated solutions so that the volume load on the circulation is maintained within tolerable limits and dilution by b~dy fluids is compensated, i.e., NMR diagnostic agents must show 100 to 1000 times the water solubility of that f~r in vitro NM~
spectroscopy. Furthermore, the agents of this invention display not only high stability so that r~lease or ex-change of the -- toxic per se -- ions not covalently bound to the complexes takes place only e~tremely gra-dually within the time wherein the contra~t media are again entirely eliminated.
The agents of this invention can also be utilized for radiation therapy. Thus, complexes of gadolinium are excellently suited for neutron capture therapy due to the large capture cross section. If the medium of this invention is intended for use in the ver-sion of radiation therapy proposed by R.L. Mills et al.
[Nature 336 : 787 (1988)~, then the centr~l ion must be derived from a Mossbauer isotope, such as, for example, s7Fe or ls1Eu In their administration, the ag3nts of this invention can also be given together with a suitable vehicle, such as, for example, serum or physiological saline solution and/or together with a protein, such as, for example, human serum albumin. The dosage herein is dependent on the type of cellular disorder and on the properties of the metal complex utilized.
Consequently, the objective has been achieved over-all of opening up novel possibilities in diagnostic medicine by means of the recited complex -ompounds.
Without further elaboration, it is believed that one skilled in the art can, using th~ preceding description, utilize the present invention to its fullest extent. The following preferred specific embodiments are, therefore, to be construed as merely illustrative, and not limitative of the remainder of the disclosure in any way whatsoever.
In the foregoing and in the following examples, all temperatures are set forth uncorrected in degrees Celsius and unless otherwise indicated, all parts and percentages are by weight.
The entire disclosures of all applic~tions, patents and publications, cited above and below, ~nd of corresponding application Federal Republi- of Germany P 39 27 444.6, filed August 16, 1989, are hereby incorporated by reference.
(~
Example 1 (a) 6-Carboxymethyl-3-ethoxycarbonylme-thyl-9-phenyl-aminocarbonylmethyl-3,6,9-triazaundecanedioic Acid __________________________________________________ At 0 C, 2.42 g (6 mmol) of N3-(2,6-dioxo-morpholinoethyl)-N6-(ethoxycarbonylmethyl)-3,6-diaza-octanedioic acid is combined in DMF with 4.16 ml (3.04 g, 30 mrnol~ of triethylamine and 559 mg (6 mmol) of aniline and stirred overnight at room temperature.
Then the clear solution is concentrated under vacuum and the residue chromatoyraphed on silica gel with dichloromethane/methanol/acetic acid/water (5:3:1:1) as the mobile phase. The combined fractions are passed over approximately 10 ml of "Amberlite" IR 120 (H+ form) and the acidic eluate is concentrated.
Yield: 2.35 g (79~) Calculated: C 53.21 H 6.50 N 11.29 Found: C 53.01 H 6.55 N 11.26 (b) 3,6,9-Tris(carboxymethyl)-3,6,9-triazaundecane-dioic Acid Phenyl Monoamide _____._________________________________________ 1.5 g (3 mmol) of the ethyl ester described in Examplel(a) is dissolved in 2N NaOH and stirred for 2 hours at room temperature. A pH of 7 is set by addin~
"Amberlite" IR 120 (H form), the mixture is filtered off, and the neutral solution is passed over about 16 ml of "Amberlite" IR 120 (H ). The acidic eluate is concentrated and additionally dried under vacuum at 50 C.
Yield: 1.3 y (92.5%) Calculated: C 51.27 H 6.03 N 11.96 Found: C 51.19 H 5.99 N 11.91 iJ IJ ~1 ?.~
(c) Gadolinium Complex of 3,6,9-Tris(carboxymethyl)-3,6,9-triazaundecanedioic Acid Phenyl Monoamide ________________________________________________ 936 mg (2 mmol) of the complexing acid obtained according to Example llb) is dissolved in 5 about 40 mi of water and combined at 80 C with 362 mg (1 mmol) of Gd2O3. After 30 minutes, the almost clear solution is filtered off and the filtrate is freeze-dried.
Yield: 1.23 g (98.8%), based on anhydrous substance.
Calculated: C 38.S7 H 4.05 N 9.00 Gd 25.25 Found: C 38.33 H 4.10 N 9.03 Gd 24.99 Example 2 Preparation of a Solution of the N-Methylglucamine Salt of the Gadolinium Complex of 3,6,9-Tris(carboxymethyl)-3,6,9-triazaundecanedioic Acid Phenyl Monoamide _______________________________________________________ 1.87 g (3 mmol) of the gadolinium complex of 3,6,9-tris(carboxymethyl)-3,6,9-triazaundecanedioic acid phenyl monoamide (Example 1) is suspended in 5 ml of water pro injectione and combined with 0.586 g (3 mmol) of N-methylglucamine, thus dissolving the complex. The mixture is filled up with water to 10 ml, the solution is introduced into a vial and subjected to heat sterilization.
Tl relaxation (ltmmol sec) is:
25 in water: 3.79 + 0.16 in plasma: 5.45 + 0.68 ~xample 3 Preparation of a So]ution of the Sodium Salt of -the Gadolinium(III) Complex of 3,6,9-Tris(carboxymethyl)-3,6,9-triazaundecanedioic Acid Phenyl Monoamide _____________________________________________________ 62.27 g (0~1 mol) of the gadolinium complex obtained according to Example l(c) is suspended in 800 ml of water pro injectione (p.i.) and dissolved at pH 7.2 by dropwise addition of normal sodium hydroxide solution. After adding 0.2 g of tromethamine, the mixture is filled up with water p.i. to 1000 ml, the solution is dispensed into bottles and heat-sterilized.
Example 4 (a) 3,6,9-Tris(carboxymethyl)-3,6,9-triazaundecanedioic Acid (N,N'-Diphenyl)diamide ___________________________________________________ 42.88 g (120 mmol) of DTPA bis-anhydride is suspended in 330 ml of dimethylormamde and cooled in an ice bath under agitation to about 5 C. Within 50 minutes, a solution of 32.9 ml (360 mmol) of aniline in 30 ml of dimethylformamide is added dropwise. The mixture is stirred for another hour in an ice bath, then overnight at room temperature. ~ter this time, a slightly turbid solution has formed. The solvent is removed under vacuum, and the smeary residue is triturated with diethyl ether to remove traces of sol-vent. The residue is combined with 500 ml of water anddissolved by adding 20 ml of llN sodium hydroxide solution. The solution is combined with 3.5 g of active carbon, filtered, and freeze-dried, thus obtaining 73.9 g of the sodium salt of the title compound as a powder.
~ J~3~) ~
(b) Gadolinium Comple~ of 3,6,9-Tris~carboxymethyl)-3,6,g-triaZaUndeCanediOiC ACid IN,N~-DiPhenY1)-diamide __________________._____________________________ 10.3 g of gadolinium oxide (30 mmol) is heated under reflux with 10. 6 ml of glacial acetic acid and 150 ml of water for 20 minu-tes. The solution is filtered through a 0.1 ~m membrane filter, combined with 35 . 3 g (60 mmol) of the ligand obtained accord-ing to 4(a), and heated for 90 minutes to 80 C. The solution is stirred with 2.1 g of active carbon for 30 minutes, filtered., and then passed in succession over an anion exchange column (200 ml IRA-410) and - 100 ml of cation exchanger (IRC-50). The eluates from the columns a filtered through a 0.1 ~m membrane filter and freeze-dried, thus obtaining 17.6 g of the title compound as a white powder..
Analysis:
Calculated: C 44.75 H 4.33 Gd 22.54 N 10.04 Found: C 44.60 H 4.44 Gd 22.42 N 9.89 Example 5 (a) 3,6,9-Tris(carboxymethyl)-3,6,9-triazaundecanedioic Acid (N,N'-Dibenzyl)diamide ___________________ _________________.. ____________ 42.88 g (120 mmol~ of DTPA bis-anhydride is suspended in 330 ml of dimethylformamide and cooled in an ice bath under agitation to 5 C. Within one hour, a solution of 39.3 ml (360 mmol) of benzylamine in 30 ml of dimethylformamide is added dropwise. The mixture is stirred for another hour in the ice bath, then overnight at room temperature. After removal of the solvent under vacuum, the residue i5 triturated with diethyl ether, co~bined with 500 ml of water, and dis-solved by adding 20 ml of llN sodium hydroxide solution.
A~ter freeze-drying, 71 g of -the sodium salt of the title compound is obtained as a light-yellow powder.
(b) Gadolinium Complex of 3,6,9-Tris~carboxymethyl)-3,6,9-triazaundecanedioic Acid (N,N'-Dibenzyl)-diamide ________________________________________________ 10.9 g of gadolinium oxide (30 mmol) is heated under reflux with 10.6 ml of glacial acetic acid and 150 ml of water for 20 minutes. The solution is filtered o~er a 0.1 ~m membrane filter, combined with 37 g (60 mmol) of the ligand obtained according to 5(a), and heated for 90 minutes to 80~ C. The solution is stirred.with 3 g of active carbon for 30 minutes, filtered, and then passed in succession over an anion exchange column (200 ml IRA-410) and 100 ml cation exchange column (100 ml IRC-50). The eluates are filtered through a 0.1 ~m membrane filter and freeze-dried, thus obtaining 18 g of the title com-pound as a white powder.
Analysis:
Calculated: C 46.33 H 4.72 Gd 21.66 N 9.65 Found: C 46.46 H 4.49 Gd 21.50 N 9.81 f; '! f ~ ~I
~ w ~J
- 2~ -Example 6 (a) 6-Carboxymethyl-3-ethoxycarbonylmethyl-9-benzyl-aminocarbonylmethyl-3,6,9-triazaundecanedioic Acid __________________________________________________ 5.04 g (12.5 mmol) of N3-(2,6-dioxomorpholino-ethyl)-N~-(ethoxycarbonylmethyl)-3,6~diazaoctanedioic acid is suspended în 65 ml o~ dimeth~lformamide, stirred in an ice bath and combined, in succession, with 8.7 ml (62.5 mmol) of triethylamine and 1.34 g (12.5 mmol) of benzylamine. The mixture is then stirred for another 2 hours in the ice bath, then overni~ht at room temperature. The solvent is removed by vacuum distillation, the residue is agitated with diisopropyl ether, suctioned off, and dried. For further purification, the residue is dissolved in such a quantity of llN sodium hydroxide solution that a pH of 7 is just attained. To this mixture is added 5 g of silica gel, the suspension is dried under vacuum, and the residue is introduced into a column of 350 g of silica gel charged with a mixture of chloroform/meth-anol/glacial acetic acid/water (1750/1050/350/350).
The product is eluted with the same solvent and, after evaporation of the solvent, 4.98 g of a colorless, smeary substance is obtained which is dissolved in 35 ml of water and passed over a column with 35 ml of the cation e~changer"IR 120". The column is washed with 70 ml of water, the combined eluates are evap-orated under vacuum, and the residue is triturated with diethyl ether, thus obtaining 2.65 g of the title compound as a white powder.
Analysis:
Calculated: C 54.11 El 6.71 N 10.97 Found: C 53.95 H 6.88 N 11.23 ~ 47 ~
(b) 3,6,9-Tris(carboxymethyl)-3,6,9-triazaundecane-dioic Acid Benzyl Monoamide _____________._________________________________ A solution of 2.26 g of the compound prepared according to Example 6(a) in 46 ml of lN
sodium hydroxide solution is allowed to stand for 2.5 hours at room temperature, and the solution is then passed over a column of 110 ml of cation exchanger "IR 120". Elution is first carried out with 200 ml of water, and this fraction is discarded. Then, 600 ml of 0.5N ammonia is used for elution; this eluate is adjusted to pH 2.3 by adding "IR 120", and the solu-tion is subjected to freeze-drying, thus obtaining 1.50 g of the title compound as a white powder.
Analysis:
Calculated: C 52.28 H 6.27 N 11.61 Found: C 52.44 H 6.32 N 11.80 (c) Gadolinium Complex of 3,6,9-1'ris(carboxymethyl)-3,6,9-triazaundecanedioic Acid Benzyl Monoamide ______________ _________________________________ 965 mg (2 mmol) of the compound produced in accordance with Example 6(b) is heated with 40 ml of water and 362 mg (1 mmol) of gadolinium oxide for one hour to 80 C. The mixture is cooled to room tempera-ture, the solution is filtered through a 0.1 ~m membrane filter, and the compound is isolated by freeze-drying, yielding 1.15 g of the title compound as a white powder.
Analysis:
Calculated: C 39.61 H 4.27 Gd 24.70 N 8.80 Found: C 39.50 H 4.48 Gd 24.61 N 8.97 ~'J ~J '~
Example 7 Dysprosium(III) Complex of 3,6,9-Tris(carboxymethyl~-3,6,9-tria~.aundecanedioic Acid senzyl Monoamide _____________________________________________________ 965 mg (2 mmol) of 3,6,9-tris(carboxyme-thyl~-3,6,9-triazaundecanedioic acid benzyl monoamide (Example 6b) is heated with 40 ml of water and 373 mg (1 mmol) of dysprosium(III) oxide for one hour to 80 C.
The mixture is cooled to room temperature, the solution is filtered through a 0.1 ~m membrane filter, and the compound is isolated by freeze-drying, thus obtaining 1.22 g of the title compound as a white powder.
Analysis:
Calculated: C 39.29 H 4.24 Dy 25.31 N 8.73 Eound: C 39.41 H 4.51 Dy 25.19 N 8.70 Example 8 (a) 6-Carboxymethyl-3-ethoxycarbonylmethyl-9-tert-butylaminocarbonylmethyl-3,6,9-triazaundecane-dioic Acid ______________________________________________ At 0 C, 2.42 g (6 mmol) of N -(2r6-dioxo-morpholinoethyl)-N6-(ethoxycarbonylmethyl)-3,6-diaza-octanedioic acid is combined in DMF with 4.16 ml (3.04 g, 30 mmol) of triethylamine and 0.64 g (6 mmol) of tert-butylamine and stirred overnight at room tem-perature. The clear solution is then concentrated under vacuum and the residue chromatographed on silica gel with dichloromethane/methanol/acetic acid/water (5~3:1:1) as eluent. The combined fractions are passed over about 10 ml of "Amberlite" IR 120 (H form), and the acidic eluate is concentrated.
Yield: 2.14 g (75~) t~ 'J
Calculated: C 50.41 H 7.62 N 1l.76 Found: C 50.26 ~l 7.66 N 11.80 (b) 3,6,9-Tris(Carboxymethyl)-3,6,9--triazaundecane-dioic Acid tert-sutyl Monoamide __.____________________________________________ 1.43 g (3 mmol~ of the ethyl ester described in Example 8(a) is dissolved in 2N NaOH and stirred Eor 2 hours at room temperature. By addition of "Amberlite" 120 (H form), the mixture is adjusted to pH 7, filtered off, and the neutral solution passed over abou-t 16 ml of "Amberlite" IR 120 (H ). The acidic eluate is concentrated and further dried at 50 C
under vacuum. Yield: 1.20 g (89%).
Calculated: C 48.21 H 7.19 N 12.49 Found: C 48.13 H 7.24 N 12.41 (c) Gadolinium Complex of 3,6,9-Tris(carboxymethyl)-3,6,9-triazaundecanedioic Acid tert-Butyl Monoamide _________ ____ ______________ ________ ___ _ ______ 897 mg (2 mmol) of the complex-forming aeid obtained according to Example 8(b) is dissolved in about 40 ml of water and combined at 80 C with 362 mg (1 mmol) of Gd2O3. After 30 minutes, the almost elear solution is filtered and the filtrate freeze-dried.
Yield: 1.19 g (99%), based on anhydrous substanee.
Calculated: C 35.87 H 4.85 N 9.30 Gd 26.09 Found: C 35.97 H 4.79 N 9.28 Gd 25.83 f~
- 2~ -Example 9 (a) 6-Carboxymethyl-3-ethoxycarbonylmethyl-9-(4-methoxyben~ylcarbamoylmethyl)-3,6,9-triazaundecanedioic Acid ______________________________ 14.10 g (35 mmol) of N -(2,6-dioxomorpholino-ethyl)-N6~(ethoxycarbonylmethyl)-3,6-diazaoctanedloic acid is combined in 175 ml of dimethylformamide at 0 C with 24.4 ml (175 mmol) of triethylamine and 4.67 ml (35 mmol) of 4-methoxybenzylamine and stirred overnight at room temperature. The solvent is extensively removed by vacuum distillation and the residue is heated to boiling with 600 ml of diisopropyl ether. After cool-ing to room temperature, the mixture is decanted off from the solvent. The residue is dissolved in 185 ml of water and passed-over a column with 140 ml of cation exchanger "IR 120" (H+ form), and the column is washed with 200 ml of water. The combined eluates are con-centrated to one-third under vacuum and then freeze-dried, yielding 16.33 g of the title compound as a white powder which still contains 1.2~ water and 1~ dimethyl-formamide.
Analysis tafter correction of solvent proportions):
Calculated: C 53.33 H 6.71 N 10.36 Found: C 53.51 H 6.78 N 10.18 .
- - ~
(b) 3,6-Bis(carboxymethyl~-9-(4-methoxybenzylcarbamoyl-methyl)-3,6,9-triazaundecanedioic Acid ___________~_______________~_______________ _______ 12.34 g (22 mmol) of the ethyl ester described in Example 9(a3 is dissolved in 200 ml of water and 20 ml of llN sodium hydroxide solution and stirred for 2 hours at room temperature. A pH of 2.3 is reached by addition of 140 ml of "Amberlite" IR 120 (H form). The mixture is filtered and the solution subjected to freeze-drying.
10 Yield: 9.61 g (85% of theory?, water content 2.18~.
Analysis (after correction of water content):
Calculated: C 51.56 H 6.29 N 10.93 Found: C 51.37 H 6.44 N 10.89 (c) Gadolinium Complex of 3,6-Bis(carboxymethyl)-9-(4-methoxybenzylcarbamoylmethyl)-3,6,9-triazaundecanedioic Acid _______________________________________________ 5.24 g (10 mmol) of the complexing acid obtained according to Example 9(b) is stirred in 200 ml of water with 1.81 g ~5 mmol? of gadolinium oxide for one hour at ~0 C, producing an almost clear solution.
The latter is filtered and the filtrate subjected to freeze-dryin~.
Yield: 6.31 g, water content 3.7%.
Analysis (after correction of water content):
25 Calculated: C 39.63 H 4.38 N 8.40 Gd 23.99 Found: C 39.88 H 4.53 N 8.51 Gd 23.70 s ~
(d) N-Methylglucamine Salt of the Gadolinium Complex of 3,6-Bis(carboxymethyl~-9-(4-methoxybenzyl-carbamoylmethyl~-3,6,9-triazaundecanedioic Acid ________________________________________________ 2 g of the gadolinium complex obtained according to Example 9(c) is dissolved in 30 ml of waterl combined with 1 equivalent of N-methylglucamine, and the solution is concentrated by evaporation under vacuum.
Yield: 2.30 g, water content 4%.
Analysis (after correction of water content):
Calculated: C 40.41 H 5.38 N 8.12 Gd 18.24 Found: C 40.52 H 5.11 N 8.27 Gd 18.39 (e) Sodium Sal-t of the Gadolinium Complex of 3,6-Bis(carboxymethyl)-~-(4-methoxybenzyl-carbamoylmethyl)-3,6,9-triazaundecanedioic Acid ____________________________________________ __ 0.5 g of the gadolinium complex obtained in accordance with Example 9(c) is dissolved in lO ml o~
water, combined with l equivalent of sodium~hydroxide dissolved in 5 ml of water, and the solution of the title compound is subjected to freeze-drying, producing 0.55 g of the title compound as a white powder with a water content of 4.5~.
Analysis (after correction of water content):
Calculated: C 38.37 H 4.10 Gd 22.83 N 8.13 Na 3.34 Found: C 38.40 H 4.45 Gd 22.43 N 8.10 Na 3.57 - . ~
~ ^ I ~ ' . ' ! ' Example 10 (a) 6-Carboxymethyl-3-ethoxycarbonylmethyl-9-(N-undecylcarbamoylmethyl)-3,6,9-triaza-undecanedioic Acid _________________________________________ Under a nitrogen atmospherel 12.10 g (30 mmol) of N3-(2,6-dioxomorpholinoethyl)-N6-(ethoxycarbonylmethyl)-3,6-diazaoctanedioic acid in 1 liter of absolute dimethylformamide is combined at 0 C with 12.6 ml (91 mmol) of triethylamine and 5.14 g (30 mmol) of l-undecylamine and stirred for 16 hours at 20-25 C. After the reaction is completed, the solvent is evaporated under vacuum and the remain-ing oily residue is stirred with 1 liter of diethyl ether. The thus-separated white powder is suctioned off, rinsed with 1 liter of diethyl ether in portions, and the product is dried at 40 C under vacuum.
Yield: 14.31 g (83%~, white powder.
Water content: 1.41 Dimethylformamide content: 0.8~
Analysis (after correction of solvent proportions):
Calculated: C 56.43 H 8.77 N 9.75 Found: C 56.25 H 8.89 N 9.48 (b) 3,6-Bis(carboxyme-thyl)-9-(N-undecylcarbamoyl-methyl)-3,6,9-triazaundecanedioic Acid __________________________________________ __ 10 g (17.4 mmol) of the ethyl ester described in Example lO~a) is dissolved in 200 ml of 2N sodium hydroxide solution and stirred for 2 hours at room temperature. After the reaction is finished, the mixture is cooled to 5 C and concentrated hydro-chloric acid is added until a pH value of 2.15 has been ~J ~ C~
attained. The thus-separated white powder i5 suctioned off and washed five times with 50 ml of ice water, five times with diethyl ether/ethanol (8:2), five times with S0 ml of diethyl ether, and five times with 50 ml of n-pentane.
Yield: 8.25 g t86.7~, white powder.
Water content: 2.12%
Dimethylformamide content: < 0.05%
Analysis (after correction of water content):
10 Calculated: C 54.93 H 8.48 N 10.25 Found: C 54.78 H 8.67 N 9.98 (c) Gadolinium Complex of 3,6-Bis(carboxymethyl)-9-(N-undecylcarbamoylmethyl)-3,6,9-tria~aundecane-dioic Acid __________________ _____________________________ At 80 C, 5.47 g (10 mmol) of the complex-forming acid obtained according to Example lO(b) is stirred in 500 ml of water with 1.81 g (5 mmol) of gadolinium oxide for 4 hours, thus producing an almost clear solution. The latter is filtered and the filtrate subjected to free~e-drying.
Yield: 6.35 g (90.6%), white powder.
Water content: 4.2%
Analysis (after correction of water content):
Calculated: C 42.84 ~1 6.18 N 7.99 Gd 22.44 25 Found: C 42.91 H 6.25 N 7.87 Gd 22.40 (d) Mono-N-methylglucamine Sal-t of the Gadolinium Complex of 3,6-Bis(carboxymethyl)-9-(N-undecyl-carbamoylmethyl)-3,6,9-triazaundecanedioic Acid 2 g of the gadolinium complex obtained in accordance with Example lO(c) is dissolved in 35 ml of water, combined with one equivalent of N-methyl-glucamine, filtered, and the solution is evaporated under vacuum.
Yield: 2.25 g (88%), white powder.
Water content: 3.75%
Analysis (after correction of water content): ~
Calculated: C 42.89 H 6.75 N 7.82 Gd 17.55 Found: C 42.73 H 6.89 N 7.69 Gd 17.48 Example 11 5 (a) 6-Carboxymethyl-3-ethoxycarbonylmethyl-9-(1-hexa-decylcarbamoylmethyl)-3,6,9-triazaundecanedioic ~cid 28.20 g (70 mmol) of N3-(2,6-dioxomorpholino-ethyl)-N6-(ethoxycarbonylmethyl)-3,6-diazaoctanedioic acid is combined in 320 ml of dimethylformamide at 0 C wi-th 28.8 ml (350 mmol) of triethylamine and 16.90 g (70 mmol) of 1-hexadecylamine and then stirred for 24 hours at 20-25 C. The mixture is therea~ter concentrated under vacuum and the residue stirred under boiling heat with 1 liter of methyl tert-butyl ether.
After cooling to +10 C, the mixture is suctioned off, the residue dried at 45 C under vacuum, taken up in 400 ml of water, and the solution is passed over a column with 300 ml of ion e~changer "IR 120" (H Porm), the column is washed with 0.5 liter of water, and the combined eluates are concentrated under vacuum to about - 3~ -300 ml, and the title compound is isolated by freeze-dryingl thus obtaining 36.5 g as a white powder.
Water content: 1.70%
Dimethylformamide conten-t: 0.7%
Analysis (after correction of solvent proportions):
Calculated: C 59.60 ll 9.38 N 8.69 Found: C 59.42 H 9.49 N 8.85 (b) 3,6-Bis(carboxymethyl)-9-(1-hexadecylcarbamoyl-methyl)-3,6,9-triazaundecanedioic Acid _______________________________________________ 6.45 g (10 mmol) of the ethyl ester disclosed in Example ll(a) is dissolved in 100 ml of water and 9.1 ml of llN sodium hydroxide solution and left for 2 hours at room temperature. Under agitation, the mix-ture is combined with 65 ml of ion exchanger "Amberlite"
IR 120 (H form), thus setting a pH of 2.5. The mix-ture is filtered and the solution is subjected to freeze-drying, thus obtaining 5.30 g of the title compound as a white powder.
Water content: 3.2%
Dimethylformamide content: ~ 0.05~
Analysis (after correction of water content):
Calculated: C 58.42 El 9.15 N 9.08 Found: C 58.59 H 9.44 N 8.95 ~c) Monomeglumine Salt of -the Gadolinium Complex of 3,6-sis(carboxymethyl~-9-(1-hexadecylcarbamoyl-methyl)-~,6,9-triazaundecanedioic Ac.id _______________________________________________ A-t 80-85 C, 4.93 g (8 mmol) of the complex-Eorming acid obtained according to Example ll(b~
is stirred in 170 ml of water for 2 hours with 1.45 g (4 mmol) of gadolinium oxide and 1.56 g (8 mmol) of N-methylglucamine. The almost clear solution is filtered and Ereeze-dried.
~ield: 7.49 g of a white powder.
Water content: 2.8~
Analysis (after correction of water content):
Calculated: C 45.99 H 7.30 N 7.25 Gd 16.27 Found: C 46.21 H 7.55 N 7.08 Gd 16.18 By the same route as described in Example 11, the monomeglumine salt of the europium complex of 3,6-bis(carboxymethyl)-9-(1-hexadecylcarbamoylmethyl3-3,6,9-triazaundecanedioic acid can be prepared as well.
Example 12 Preparation of a Solution of the Meglumine Salt of the Gadolinium Complex of 3,6,9-Tris(carboxymethyl)-3,6,9-tri.azaundecanedioic Acid Undecyl Monoamide _____________________________________________________._ 448.57 g (0.5 mol) of the compound described in Example 10 ~d) is dissolved under heating in 600 ml o~
water pro injectione (p.i.). After addition of 4.92 g (10 mmol) of the monohydrate of the calciumtrisodium salt of DTPA, CaNa3DTPA, tlle solution is filled up with water p.i. to 1000 ml. The solution is subjected to ultrafiltration, dispensed into bo-tt].es, and heat-sterilized, and is ready ~or use :Eor parenteral admini-stration.
C 1 ' " ! ' ' Example 13 Production of a Powder Form of Administration 89.61 g (0.1 mol) o~ the meglumine salt disclosed in Example lO(d) is finely ground up with 25 g of sucrose and 5 g of "Pluronic" F 68 and lo mg of raspberry flavoring. The powder is filled into bags and is ready for oral administration.
The preceding examples can be repeated with similar success by substituting the generically or specifically described reactants and/or operating conditions of this invention for those used in the preceding examples.
From the foregoing description, one skilled in the art can easily ascertain the essential ch~racteristics of this invention, and without departing from the spirit and scope thereof, can make various changes and modifications of the invention to adapt it to various usages and conditions.
Claims (31)
1. In a method of NMR imaging, the improvement comprising administering an organ-specific contrast agent wherein said agent comprises a compound of the formula (I) wherein n is O, 1 or 2;
R1 and R2 are each independent H, C1-8-alkyl, phenyl, benzyl, or, if n is O, can also jointly form trimethylene or tetramethylene;
R3 is an aliphatic hydrocarbon of up to 16 C atoms and, when R4 is H, at least one R3 is C3.16-cycloalkyl,C6,10-aryl,C6-10-aryl-C1-6-alkyl, or C6-10-aryl or C6-10-aryl-C1-6-alkyl substituted by one or more di-C1-6-alkylamino or one or more C1-6-alkoxy;
R4 is H or aliphatic hydrocarbon of up to 16 C
atoms; or R3 and R4 jointly form a saturated or unsaturated 5- or 6-membered ring, optionally containing a N, O
or S atom and/or optionally substituted by oxo, C1-6-alkyl, C1-5-hydroxyalkyl, c2-6(,-alkanoyl, C2-6-alkanoyl substituted by OH, C26-alkanoyl substituted by C1-6-alkyl, hydroxy, carbamoyl, C16-alkyl substituted by carbamoyl, carbamoyl substituted on the carbamoyl nitrogen by one or two C16-alkyl group(s) -- the latter optionally together form a ring which optionally contains an 0 atom, or a C1-C6-acylamino or C1-C6-alkylamino;
X is H and/or a metal ion equivalent of at least one element of atomic numbers 21-29, 42, 44 or 58-70, with the proviso that at least two of the X groups represent a metal ion equivalent;
and Y is a COOX- or ; or physiologically acceptable salt thereof with organic and/or inorganic base.
R1 and R2 are each independent H, C1-8-alkyl, phenyl, benzyl, or, if n is O, can also jointly form trimethylene or tetramethylene;
R3 is an aliphatic hydrocarbon of up to 16 C atoms and, when R4 is H, at least one R3 is C3.16-cycloalkyl,C6,10-aryl,C6-10-aryl-C1-6-alkyl, or C6-10-aryl or C6-10-aryl-C1-6-alkyl substituted by one or more di-C1-6-alkylamino or one or more C1-6-alkoxy;
R4 is H or aliphatic hydrocarbon of up to 16 C
atoms; or R3 and R4 jointly form a saturated or unsaturated 5- or 6-membered ring, optionally containing a N, O
or S atom and/or optionally substituted by oxo, C1-6-alkyl, C1-5-hydroxyalkyl, c2-6(,-alkanoyl, C2-6-alkanoyl substituted by OH, C26-alkanoyl substituted by C1-6-alkyl, hydroxy, carbamoyl, C16-alkyl substituted by carbamoyl, carbamoyl substituted on the carbamoyl nitrogen by one or two C16-alkyl group(s) -- the latter optionally together form a ring which optionally contains an 0 atom, or a C1-C6-acylamino or C1-C6-alkylamino;
X is H and/or a metal ion equivalent of at least one element of atomic numbers 21-29, 42, 44 or 58-70, with the proviso that at least two of the X groups represent a metal ion equivalent;
and Y is a COOX- or ; or physiologically acceptable salt thereof with organic and/or inorganic base.
2. A method according to claim 1, wherein n is 1;
R1 and R2 are each H:
R3 is an aliphatic hydrocarbon of up to 16 C atoms and, when R4 is H, at least one R3 is C3-16-cycloalkyl, C6,10-aryl, C6-10-aryl-C1-6-alkyl, or C6-10-aryl or C6-10-aryl-C1-6-alkyl substituted by one or more di-C1-6-alkylamino or one or more C16-alkoxy; and R4 is H or an aliphatic hydrocarbon of up to 16 C
atoms.
R1 and R2 are each H:
R3 is an aliphatic hydrocarbon of up to 16 C atoms and, when R4 is H, at least one R3 is C3-16-cycloalkyl, C6,10-aryl, C6-10-aryl-C1-6-alkyl, or C6-10-aryl or C6-10-aryl-C1-6-alkyl substituted by one or more di-C1-6-alkylamino or one or more C16-alkoxy; and R4 is H or an aliphatic hydrocarbon of up to 16 C
atoms.
3. A method according to claim l, wherein Y is COOX- .
4. A method according to claim 1, wherein Y is
5. A method according to claim 2, wherein Y is COOX-.
6. A method according to claim 2, wherein Y is .
7. A method according to claim 1, wherein R4 is H
and R3 is C3-16-cycloalkyl, C6-10-aryl, C6-10-aryl-C1-6 alkyl, or C6-10 aryl or C6-10-aryl-C1-6-alkyl substituted by one or more di-C1-C6-alkylamino or one or more C1-6-alkoxy.
and R3 is C3-16-cycloalkyl, C6-10-aryl, C6-10-aryl-C1-6 alkyl, or C6-10 aryl or C6-10-aryl-C1-6-alkyl substituted by one or more di-C1-C6-alkylamino or one or more C1-6-alkoxy.
8. A method according to claim 1, wherein R4 is H
and R3 is C3-16-cycloalkyl, C6-10-aryl, C6-10-ary1-C1-6-alky, or C6-10 aryl or C6-10-aryl-C1-6-alkyl substituted by one or more di-C1-C6-alkylamino or one or more C1-6-alkoxy.
and R3 is C3-16-cycloalkyl, C6-10-aryl, C6-10-ary1-C1-6-alky, or C6-10 aryl or C6-10-aryl-C1-6-alkyl substituted by one or more di-C1-C6-alkylamino or one or more C1-6-alkoxy.
9. A method according to claim 1, wherein said complex is:
(a) gadolinium complex of 3,6,9-tris(carboxymethyl)-3,6,9-triazaundecanedioic acid phenyl monoamide;
(b) gadolinium complex of 3,6,9-tris(carboxymethyl)-3,6,9-triazaundecanedioic acid (N,N'-diphenyl)diamide;
(c) gadolinium complex of 3,6,9-tris(carboxymethyl)-3,6,9-triazaundecanedioic acid (N,N'-dibenzyl)diamide;
(d) gadolinium complex of 3,6,9-tris(carboxymethyl)-3,6,9-triazaundecanedioic acid benzyi monoamide;
(e) dysprosium(III) complex of 3,6,9-tris(carboxymethyl)-3,6,9-triazaundecanedioic acid benzyl monoamide;
(f) gadolinium complex of 3,6,9-tris(carboxymethyl)-3,6,9-triazaundecanedioic acid tert-butyl monoamide;
(g) gadolinium complex of 3,6,9-bis(carboxymethyl)-9-(4-methoxybenzylcarbamoylmethyl)-3,6,9-triazaundecanedioic acid;
(h) gadolinium complex of 3,6-bis(carboxymethyl)-9-(N-undecylcarbamoylmethyl)-3,6,9-triazaundecanedioic acid;
(i) gadolinium complex of 3,6-bis(carboxymethyl)-9-(1-hexadecylcarbamoylmethyl)-3,6,9-triazaundecanedioic acid; or a physiologically acceptable salt of anyone of (a)-(h) with organic and/or inorganic base.
(a) gadolinium complex of 3,6,9-tris(carboxymethyl)-3,6,9-triazaundecanedioic acid phenyl monoamide;
(b) gadolinium complex of 3,6,9-tris(carboxymethyl)-3,6,9-triazaundecanedioic acid (N,N'-diphenyl)diamide;
(c) gadolinium complex of 3,6,9-tris(carboxymethyl)-3,6,9-triazaundecanedioic acid (N,N'-dibenzyl)diamide;
(d) gadolinium complex of 3,6,9-tris(carboxymethyl)-3,6,9-triazaundecanedioic acid benzyi monoamide;
(e) dysprosium(III) complex of 3,6,9-tris(carboxymethyl)-3,6,9-triazaundecanedioic acid benzyl monoamide;
(f) gadolinium complex of 3,6,9-tris(carboxymethyl)-3,6,9-triazaundecanedioic acid tert-butyl monoamide;
(g) gadolinium complex of 3,6,9-bis(carboxymethyl)-9-(4-methoxybenzylcarbamoylmethyl)-3,6,9-triazaundecanedioic acid;
(h) gadolinium complex of 3,6-bis(carboxymethyl)-9-(N-undecylcarbamoylmethyl)-3,6,9-triazaundecanedioic acid;
(i) gadolinium complex of 3,6-bis(carboxymethyl)-9-(1-hexadecylcarbamoylmethyl)-3,6,9-triazaundecanedioic acid; or a physiologically acceptable salt of anyone of (a)-(h) with organic and/or inorganic base.
10. A method according to claim 1, wherein said agent further comprises a pharmaceutically acceptable carrier.
11. A method according to claim 1, wherein said agent is sterile.
12. A method according to claim 1, wherein said agent further comprises additional separate complexing agent.
13. A method according to claim 1, wherein said agent further comprises a physiologically acceptable buffer, an electrolyte, and/or an antioxidant.
14. A method according to claim 1, wherein said agent is administered orally.
15. A method according to claim 14, wherein said complex is administered at a concentration of 0.1 mmol/1-100 mmol/1.
16. A method according to claim 1, wherein said agent is administered intravenously.
17. A method according to claim 16, wherein said complex is administered at a concentration of 50 µmol/1-2 mol/1.
18. A method according to claim 1, wherein said complex is administered at a dosage of 1 µmol-5 mmol per kg of body weight.
19. A method according to claim 1, wherein said agent is administered to a human.
20. A method according to claim 1, wherein the renal system is imaged.
21. A method according to claim 1, wherein the liver is imaged.
22. A method according to claim 1, wherein the gall bladder is imaged.
23. A method according to claim 1, wherein bile duct(s) are imaged.
24. A method according to claim 1, wherein stomach ulcer(s) are imaged.
25. A method according to claim 1, wherein stomach carcinoma (5) are imaged.
26. In a method of organ-specific NMR imaging, the improvement comprises administering a contrast agent wherein said agent comprises a physiologically compatible complex compound of the formula (I) wherein n is o, 1 or 2;
R1 and R2 are each independent H, C1-8-alkyl, phenyl, benzyl, or, if n is 0, can also jointly form trim ethylene or tetramethylene;
R3 is an aliphatic hydrocarbon of up to 16 C atoms and, when R4 is H, at least one R3 is C3-16- cycloalkyl, C6-10-aryl, C6-10-aryl-C1-6-alkyl, or C6-10-aryl or C6-10-aryl-C1-6-alkyl substituted by one or more di-C1-6-alkylamino or one or more C1-6-alkoxy;
R4 is H or aliphatic hydrocarbon of up to 16 C
atoms; or R3 and R4 jointly form a saturated or unsaturated 5- or 6-membered ring, optionally containing a N, O
or S atom and/or optionally substituted by oxo, C1-6-alkyl, C1-5-hydroxyalkyl, C2-6-alkanoyl, C2-6-alkanoyl substituted by OH, C2-6-alkanoyl substituted by C1-6-alkyl, hydroxy, carbamoyl, C1-6-alkyl substituted by carbamoyl, carbamoyl substituted on the carbamoyl nitrogen by one or two C1-6-alkyl group(s) -- the latter optionally together form a ring which optionally contains an O atom, or a C1-6-acylamino or C1-6-alkylamino;
X is H and/or a metal ion equivalent of at least one element of atomic numbers 21-29, 42, 44 or 58-70, with the proviso that at least two of the X groups represent a metal ion equivalent;
or Y is a COOX- or ; or physiologically acceptable salt thereof with organic and/or inorganic base.
R1 and R2 are each independent H, C1-8-alkyl, phenyl, benzyl, or, if n is 0, can also jointly form trim ethylene or tetramethylene;
R3 is an aliphatic hydrocarbon of up to 16 C atoms and, when R4 is H, at least one R3 is C3-16- cycloalkyl, C6-10-aryl, C6-10-aryl-C1-6-alkyl, or C6-10-aryl or C6-10-aryl-C1-6-alkyl substituted by one or more di-C1-6-alkylamino or one or more C1-6-alkoxy;
R4 is H or aliphatic hydrocarbon of up to 16 C
atoms; or R3 and R4 jointly form a saturated or unsaturated 5- or 6-membered ring, optionally containing a N, O
or S atom and/or optionally substituted by oxo, C1-6-alkyl, C1-5-hydroxyalkyl, C2-6-alkanoyl, C2-6-alkanoyl substituted by OH, C2-6-alkanoyl substituted by C1-6-alkyl, hydroxy, carbamoyl, C1-6-alkyl substituted by carbamoyl, carbamoyl substituted on the carbamoyl nitrogen by one or two C1-6-alkyl group(s) -- the latter optionally together form a ring which optionally contains an O atom, or a C1-6-acylamino or C1-6-alkylamino;
X is H and/or a metal ion equivalent of at least one element of atomic numbers 21-29, 42, 44 or 58-70, with the proviso that at least two of the X groups represent a metal ion equivalent;
or Y is a COOX- or ; or physiologically acceptable salt thereof with organic and/or inorganic base.
27. A method according to claim 26, wherein n is 1;
R1 and R2 are each H;
R3 is an aliphatic hydrocarbon of up to 16 C atoms and, when R4 is H, at least one R3 is C3-16-cycloalkyl, C6-10-aryl, C6-1-aryl C1-6-alkyl, or C6-10-aryl or C6-10-aryl-C1-6-alkyl substituted by one or more di-C1-6-alkylamino or one or more C1-6-alkoxy; and R4 is H or an aliphatic hydrocarbon of up to 16 c atoms.
R1 and R2 are each H;
R3 is an aliphatic hydrocarbon of up to 16 C atoms and, when R4 is H, at least one R3 is C3-16-cycloalkyl, C6-10-aryl, C6-1-aryl C1-6-alkyl, or C6-10-aryl or C6-10-aryl-C1-6-alkyl substituted by one or more di-C1-6-alkylamino or one or more C1-6-alkoxy; and R4 is H or an aliphatic hydrocarbon of up to 16 c atoms.
28. In a method of NMR imaging, the improvement comprises administering to a patient with renal insufficiency a contrast agent wherein said agent comprises a physiologically compatible complex compound of the formula (I) wherein n is 0, l or 2;
R1 and R2 are each independent H, C1-8-alkyl, phenyl, benzyl, or, if n is 0, can also jointly form trimethylene or tetramethylene;
R3 is an aliphatic hydrocarbon of up to 16 C atoms and, when R4 is H, at least one R3 is C3-16-cycloalkyl, C6-10-aryl, C6-10-aryl-C1-6-alkyl, or C6-10-aryl or C6-10-aryl-C1-6-alkyl substituted by one or more di-C1-6-alkylamino or one or more C1-6-alkoxy;
R4 is H or aliphatic hydrocarbon of up to 16 C
atoms; or R3 and R4 jointly form a saturated or unsaturated 5- or 6-membered ring, optionally containing a N, 0 or S atom and/or optionally substituted by oxo, C1-6-alkyl, C1-5-hydroxyalkyl, C2-6-alkanoyl, C2-6-alkanoyl substituted by OH, C2-6-alkanoyl substituted by C1-6-alkyl, hydroxy, carbamoyl, C1-6-alkyl substituted by carbamoyl, carbamoyl substituted on the carbamoyl nitrogen by one or two C1-6-alkyl group(s) -- the latter optionally together form a ring which optionally contains an 0 atom, or a C1-6-acylamino or C1-6-alkylamino;
X is H and/or a metal ion equivalent of at least one element of atomic numbers 21-29, 42, 44 or 58-70, with the proviso that at least two of the X groups represent a metal ion equivalent;
or Y is a COOX- or ; or physiologically acceptable salt thereof with organic and/or inorganic base.
R1 and R2 are each independent H, C1-8-alkyl, phenyl, benzyl, or, if n is 0, can also jointly form trimethylene or tetramethylene;
R3 is an aliphatic hydrocarbon of up to 16 C atoms and, when R4 is H, at least one R3 is C3-16-cycloalkyl, C6-10-aryl, C6-10-aryl-C1-6-alkyl, or C6-10-aryl or C6-10-aryl-C1-6-alkyl substituted by one or more di-C1-6-alkylamino or one or more C1-6-alkoxy;
R4 is H or aliphatic hydrocarbon of up to 16 C
atoms; or R3 and R4 jointly form a saturated or unsaturated 5- or 6-membered ring, optionally containing a N, 0 or S atom and/or optionally substituted by oxo, C1-6-alkyl, C1-5-hydroxyalkyl, C2-6-alkanoyl, C2-6-alkanoyl substituted by OH, C2-6-alkanoyl substituted by C1-6-alkyl, hydroxy, carbamoyl, C1-6-alkyl substituted by carbamoyl, carbamoyl substituted on the carbamoyl nitrogen by one or two C1-6-alkyl group(s) -- the latter optionally together form a ring which optionally contains an 0 atom, or a C1-6-acylamino or C1-6-alkylamino;
X is H and/or a metal ion equivalent of at least one element of atomic numbers 21-29, 42, 44 or 58-70, with the proviso that at least two of the X groups represent a metal ion equivalent;
or Y is a COOX- or ; or physiologically acceptable salt thereof with organic and/or inorganic base.
29. A method according to claim 28, wherein n is 1;
R1 and R2 are each H;
is an aliphatic hydrocarbon of up to 16 C atoms and, when R4 is H, at least one R3 is C3-16-cycloalkyl, C6-10-aryl, C6-10-aryl-C1-6-alkyl, or C6-10-aryl or C6-10-aryl-C1-6-alkyl substituted by one or more di-C1-6-alkylamino or one or more C1-6-alkoxy; and R4 is H or an aliphatic hydrocarbon of up to 16 C
atoms.
R1 and R2 are each H;
is an aliphatic hydrocarbon of up to 16 C atoms and, when R4 is H, at least one R3 is C3-16-cycloalkyl, C6-10-aryl, C6-10-aryl-C1-6-alkyl, or C6-10-aryl or C6-10-aryl-C1-6-alkyl substituted by one or more di-C1-6-alkylamino or one or more C1-6-alkoxy; and R4 is H or an aliphatic hydrocarbon of up to 16 C
atoms.
30. In a method of NMR imaging, the improvement comprises administering to a patient with a gastrointestinal disorder a contrast agent wherein said agent comprises a physiologically compatible complex compound of the formula (I) wherein n is O, 1 or 2;
R1 and R2 are each independent H, C1-8-alkyl, phenyl, benzyl, or, if n is 0, can also jointly form trimethylene or tetramethylene;
R3 is an aliphatic hydrocarbon of up to 16 C atoms and, when R4 is H, at least one R3 is C3-16-cycloalkyl, C6-10-aryl, C6-10-aryl-C1-6-alky or C6-10-aryl or C6-10-aryl-C1-6-alkyl substituted by one or more di-C1-6-alkylamino or one or more C1-6-alkoxy;
R4 is H or aliphatic hydrocarbon of up to 16 C
atoms; or R3 and R4 jointly form a saturated or unsaturated 5- or 6-membered ring, optionally containing a N, O
or S atom and/or optionally substituted by oxo, C1-6-alkyl, C1-5-hydroxyalkyl, C2-6-alkanoyl, C-2-6-alkanoyl substituted by OH, C2-6-alkanoyl substituted by C1-6-alkyl, hydroxy, carbamoyl, C1-6-alkyl substituted by carbamoyl, carbamoyl substituted on the carbamoyl nitrogen by one or two C1-6-alkyl group(s) -- the latter optionally together form a ring which optionally contains an O atom, or C1-6-acylamino or C1-6-alkylamino;
X is H and/or a metal ion equivalent of at least one element of atomic numbers 21-29, 42, 44 or 58-70, with the proviso that at least two of the X groups represent a metal ion equivalent;
Y is a COCK- or ; or physiologically acceptable salt thereof with organic and/or inorganic base.
R1 and R2 are each independent H, C1-8-alkyl, phenyl, benzyl, or, if n is 0, can also jointly form trimethylene or tetramethylene;
R3 is an aliphatic hydrocarbon of up to 16 C atoms and, when R4 is H, at least one R3 is C3-16-cycloalkyl, C6-10-aryl, C6-10-aryl-C1-6-alky or C6-10-aryl or C6-10-aryl-C1-6-alkyl substituted by one or more di-C1-6-alkylamino or one or more C1-6-alkoxy;
R4 is H or aliphatic hydrocarbon of up to 16 C
atoms; or R3 and R4 jointly form a saturated or unsaturated 5- or 6-membered ring, optionally containing a N, O
or S atom and/or optionally substituted by oxo, C1-6-alkyl, C1-5-hydroxyalkyl, C2-6-alkanoyl, C-2-6-alkanoyl substituted by OH, C2-6-alkanoyl substituted by C1-6-alkyl, hydroxy, carbamoyl, C1-6-alkyl substituted by carbamoyl, carbamoyl substituted on the carbamoyl nitrogen by one or two C1-6-alkyl group(s) -- the latter optionally together form a ring which optionally contains an O atom, or C1-6-acylamino or C1-6-alkylamino;
X is H and/or a metal ion equivalent of at least one element of atomic numbers 21-29, 42, 44 or 58-70, with the proviso that at least two of the X groups represent a metal ion equivalent;
Y is a COCK- or ; or physiologically acceptable salt thereof with organic and/or inorganic base.
31. A method according to claim 30, wherein n is 1;
R1 and R2 are each H;
R3 is an aliphatic hydrocarbon of up to 16 C atoms and, when R4 is H, at least one R3 is C3-16-cycloalkyl, C6-10-aryl, C6-10- aryl-C1-6-alkyl, or C6-10-aryl or C6-10-aryl-C1-6-alkyl substituted by one or more di-C1-6-alkylamino or one or more C1-6-alkoxy; and R4 is H or an aliphatic hydrocarbon of up to 16 C
atoms.
R1 and R2 are each H;
R3 is an aliphatic hydrocarbon of up to 16 C atoms and, when R4 is H, at least one R3 is C3-16-cycloalkyl, C6-10-aryl, C6-10- aryl-C1-6-alkyl, or C6-10-aryl or C6-10-aryl-C1-6-alkyl substituted by one or more di-C1-6-alkylamino or one or more C1-6-alkoxy; and R4 is H or an aliphatic hydrocarbon of up to 16 C
atoms.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA 2023363 CA2023363A1 (en) | 1989-08-16 | 1990-08-15 | Use of amide complex compounds |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DEP3927444.6 | 1989-08-16 | ||
CA 2023363 CA2023363A1 (en) | 1989-08-16 | 1990-08-15 | Use of amide complex compounds |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2023363A1 true CA2023363A1 (en) | 1991-02-17 |
Family
ID=4145743
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA 2023363 Abandoned CA2023363A1 (en) | 1989-08-16 | 1990-08-15 | Use of amide complex compounds |
Country Status (1)
Country | Link |
---|---|
CA (1) | CA2023363A1 (en) |
-
1990
- 1990-08-15 CA CA 2023363 patent/CA2023363A1/en not_active Abandoned
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US5650133A (en) | Macrocyclic polyaza dichelates linked through ring nitrogens via an amide or ester functionality | |
US5693310A (en) | Amide complexes | |
EP0255471B1 (en) | 1,4,7,10-tetraazacyclododecane-derivatives | |
AU627451B2 (en) | Mesotetraphenylporphyrin complex compounds, process for their production and pharmaceutical agents containing them | |
US5277895A (en) | Mono-n-substituted 1,4,7,10-tetraazacyclododecane derivatives, process for their production and pharmaceutical agents containing these derivatives | |
IE902964A1 (en) | Use of amide complex compounds | |
US5399340A (en) | Use of amide complex compounds | |
RU2743167C2 (en) | Contrast agents | |
CA1341035C (en) | Macrocyclic polyaza compounds containing 5 or 6 membered rings, process for producing them and pharmaceutical media containing them. | |
HUT61306A (en) | Process for producing new chelating agents and pharmaceutical compositions comprising same as active ingredient | |
MXPA01011439A (en) | Bicyclic polyaminoacid metal complexes, method for preparing same and use in medical imaging. | |
NO180582B (en) | Diethylenetriamine acetic acid monoamides (DTPA monoamides), complexes and complex salts thereof, and use of the compounds for the preparation of agents for NMR, X-ray or radio diagnostics | |
EP0722442B1 (en) | Polyazacycloalkanes as dichelants | |
EP3442949B1 (en) | Contrast agents | |
US6083479A (en) | Contrast media for infarction and necrosis imaging | |
EP0717737B1 (en) | Chelants as contrast enhancing agents | |
EP0250358A2 (en) | Novel complex compounds | |
CA2095727A1 (en) | Chelating agents | |
CA2023363A1 (en) | Use of amide complex compounds | |
CA1341289C (en) | Amide complexes | |
CA2177271A1 (en) | 1,4,7,10-tetraazacyclododecane derivatives, their use, pharmaceuticals containing them and process for their production | |
EP3386953B1 (en) | Contrast agents |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
FZDE | Dead |