CA2012632A1 - Process for the expression of a recombinant gene - Google Patents

Process for the expression of a recombinant gene

Info

Publication number
CA2012632A1
CA2012632A1 CA2012632A CA2012632A CA2012632A1 CA 2012632 A1 CA2012632 A1 CA 2012632A1 CA 2012632 A CA2012632 A CA 2012632A CA 2012632 A CA2012632 A CA 2012632A CA 2012632 A1 CA2012632 A1 CA 2012632A1
Authority
CA
Canada
Prior art keywords
recombinant gene
expression
codons
gene
aga
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA2012632A
Other languages
French (fr)
Other versions
CA2012632C (en
Inventor
Ulrich Brinkmann
Ralf Mattes
Anne Stern
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Publication of CA2012632A1 publication Critical patent/CA2012632A1/en
Application granted granted Critical
Publication of CA2012632C publication Critical patent/CA2012632C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6462—Plasminogen activators u-Plasminogen activator (3.4.21.73), i.e. urokinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/0102—Alpha-glucosidase (3.2.1.20)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21073—Serine endopeptidases (3.4.21) u-Plasminogen activator (3.4.21.73), i.e. urokinase

Abstract

For the expression of a recombinant gene which contains AGA or/and AGG codons for arginine in E. coli after transformation with an expression vector which contains the recombinant gene, where the recombinant gene contains more than 3% AGA or/and AGG codons the amount of these codons is reduced to not more than 3% based on the total amount of the codons in the gene. <IMAGE>
CA002012632A 1989-03-23 1990-03-20 Process for the expression of a recombinant gene Expired - Lifetime CA2012632C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3909710A DE3909710A1 (en) 1989-03-23 1989-03-23 METHOD FOR EXPRESSING A RECOMBINANT GENE
DEP3909710.2 1989-03-23

Publications (2)

Publication Number Publication Date
CA2012632A1 true CA2012632A1 (en) 1990-09-23
CA2012632C CA2012632C (en) 1998-07-28

Family

ID=6377123

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002012632A Expired - Lifetime CA2012632C (en) 1989-03-23 1990-03-20 Process for the expression of a recombinant gene

Country Status (8)

Country Link
EP (1) EP0388963B1 (en)
JP (1) JPH07102146B2 (en)
AT (1) ATE106099T1 (en)
CA (1) CA2012632C (en)
DE (2) DE3909710A1 (en)
DK (1) DK0388963T3 (en)
ES (1) ES2054129T3 (en)
IL (1) IL93851A (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6335160B1 (en) * 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
EP2275559A3 (en) * 1999-09-28 2011-03-23 Shire Human Genetic Therapies, Inc. Optimized messenger RNA
WO2001068835A2 (en) * 2000-03-13 2001-09-20 Aptagen Method for modifying a nucleic acid
JP3910142B2 (en) 2000-09-14 2007-04-25 タカラバイオ株式会社 Thermostable ribonuclease H
TWI247804B (en) 2000-09-26 2006-01-21 Takara Bio Inc Sphingolipid ceramide deacylase gene
WO2005118815A1 (en) 2004-06-04 2005-12-15 Takara Bio Inc. Polypeptides having dna polymerase activity
JP2011500036A (en) * 2007-10-15 2011-01-06 ザ ユニバーシティー オブ クイーンズランド Construct systems and their use

Also Published As

Publication number Publication date
ES2054129T3 (en) 1994-08-01
DE3909710A1 (en) 1990-09-27
IL93851A (en) 1999-01-26
EP0388963B1 (en) 1994-05-25
ATE106099T1 (en) 1994-06-15
DK0388963T3 (en) 1994-08-29
JPH07102146B2 (en) 1995-11-08
DE59005790D1 (en) 1994-06-30
CA2012632C (en) 1998-07-28
EP0388963A1 (en) 1990-09-26
IL93851A0 (en) 1990-12-23
JPH02286086A (en) 1990-11-26

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