CA2004422A1 - Process for the stabilization of chloramines or salts of hypochlorous acid - Google Patents
Process for the stabilization of chloramines or salts of hypochlorous acidInfo
- Publication number
- CA2004422A1 CA2004422A1 CA 2004422 CA2004422A CA2004422A1 CA 2004422 A1 CA2004422 A1 CA 2004422A1 CA 2004422 CA2004422 CA 2004422 CA 2004422 A CA2004422 A CA 2004422A CA 2004422 A1 CA2004422 A1 CA 2004422A1
- Authority
- CA
- Canada
- Prior art keywords
- salts
- chloramines
- stabilization
- hypochlorous acid
- chloramine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C303/00—Preparation of esters or amides of sulfuric acids; Preparation of sulfonic acids or of their esters, halides, anhydrides or amides
- C07C303/42—Separation; Purification; Stabilisation; Use of additives
-
- C—CHEMISTRY; METALLURGY
- C01—INORGANIC CHEMISTRY
- C01B—NON-METALLIC ELEMENTS; COMPOUNDS THEREOF; METALLOIDS OR COMPOUNDS THEREOF NOT COVERED BY SUBCLASS C01C
- C01B11/00—Oxides or oxyacids of halogens; Salts thereof
- C01B11/04—Hypochlorous acid
- C01B11/06—Hypochlorites
- C01B11/068—Stabilisation by additives other than oxides, hydroxides, carbonates of alkali or alkaline-earth metals; Coating of particles; Shaping; Granulation
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Geology (AREA)
- Inorganic Chemistry (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Medicinal Preparation (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
ABSTRACT OF THE INVENTION
A process for the stabilization of chloramines or salts of hypochlorous acid in dried form using oxidation-resistant reversibly dehydratable substances is described.
A process for the stabilization of chloramines or salts of hypochlorous acid in dried form using oxidation-resistant reversibly dehydratable substances is described.
Description
2~ 4~2 BEHRINGWERKE AXTIENGESELLSCHAFT 88/B 040 - Na 741 Dr. Ha/Sd ~ . ~. ...
Process for the stabilization of chloramines or salts of hypochlorous acid : ~. .. :: .
The invention relates to a process for the stabilization of chloramines or salts of hypochlorous acid in dried form using reversibly dehydratable oxidation-resistant substances. -Chloramines a6 agents oxidizing methionine to methionine sulfoxide are of importance in the clinical chemistry of fibrinolysis. They make it possible to measure fibrino-lytic activity virtually independent of the presence of alpha-2-antiplasmin.
. ;. - .. ,::
Solutions of chloramines, however, can be kept only for a limited period, especially when oxidizable substances are present. It is therefore useful to provide the chlor-amines in lyophilised form. Common lyophilisation fillers such as mannitol or RFicoll are, however, unsuitable, as they react with oxidants.
: ;, . -;
It is therefore the aim of the invention to provide a process for the stabilization of chloramines.
Surprisingly, oxidants of the chloramine type, such a8 chloramine-T, chloramine-L or their salts or salts of HOCl in dried, preferably lyophilised form, are stabilized by reversibly dehydratable oxidation-resistant substances.
The invention thus relates to a process for the stabi-lization of chlor~mines or salts of hypochlorous acid in dried form, comprising the addition of an oxidation-resistant reversibly dehydratable substance.
Preferred substances are sugars, preferably maltose, or salts, preferably trisodium citrate dihydrate or calcium '.'':'~, 2~0~ Z
- 2 - ~:
acetate.
The substance 6uch as sugars, preferably maltose, or salts are employed in concentrations from 0.1-30, prefer-ably 1-5% (per cent by weight).
In lyophilised form, the oxidants stabilized in this way retain their oxidative strength even if the reagents are stored for more than two weeks at 37C.
The invention is further defined in the claims and is described in detail in the following example .
. . ''~ ``., '~
2(~ 4;~2 - 3 - :
Example Stabilization of chloramine by hygroscopic substances 450.7 mg chloramine-T (or 341.7 mq chloramine-B) were taken up in 20 ml of distilled water together with 94.3 mg of tranexamic acid and 600 mg in each case of - :~
maltose monohydrate, calcium acetate or trisodium citrate dihydrate and the pH-values were ad~usted to pH 10.
.: ::- ~:
After complete dissolution the solutions were used in the ~ehringwerke PAI-CAP test as a substitute for the "Oxidant" reagent. Additionally, the solutions were lyo-philised in portions of 1 ml and stored in solid (lyo-philised) form and in dissolved form at 37C and a PAI-CAP test was carried out with these reagents. Tris buffer or "Standard Sl" from the Behringwerke PAI-test kit was employed as the sample in each case. The results are given in the following table.
.: :: : . ~.-:, ,. .,: :,' .,. :' ..',,: ,., ::
. ,:: : . :. :. , ... ~:
...... ,.~,.. ~
,,;,"- ,,;~
, :, ~ " ;..-- - . . - , . .
. .
200~4~2 Table -chloramine-T chloramine-B .
measured plasmin act. (A/5 min).x 1000 :-buffer Standard Sl act. decrease in Std.
Sl respect.to buffer(%) ~:~
maltose immediate 1.298 1.210 7 10 after 1 week 1.271 1.076 15 23 after 2 weeks 1.078 0.909 16 25 .
calcium acetate immediate 1.262 1.156 8 15 ~ . :
after 1 week 1.212 1.051 13 23 .
after 2 weeks 1.160 1.002 14 22 trisodium citrate dihydrate immediate 1.363 1.205 12 15 --~
after 1 week 1.304 1.036 21 25 : .;~
after 2 weeks 1.190 0.971 18 20 : .
. . .
Mean values from two determinations ~ :~
Maltose, calcium acetate or trisodium citrate dihydrate are found to be almost inert towards oxidation with re-'' ''"'~,'!.~ "~`' spect to chloramines, which they stabilize.
. ,'. ','.' ~.,~
"
X
..: , - ..~, .....
,, ~. : , , ' '
Process for the stabilization of chloramines or salts of hypochlorous acid : ~. .. :: .
The invention relates to a process for the stabilization of chloramines or salts of hypochlorous acid in dried form using reversibly dehydratable oxidation-resistant substances. -Chloramines a6 agents oxidizing methionine to methionine sulfoxide are of importance in the clinical chemistry of fibrinolysis. They make it possible to measure fibrino-lytic activity virtually independent of the presence of alpha-2-antiplasmin.
. ;. - .. ,::
Solutions of chloramines, however, can be kept only for a limited period, especially when oxidizable substances are present. It is therefore useful to provide the chlor-amines in lyophilised form. Common lyophilisation fillers such as mannitol or RFicoll are, however, unsuitable, as they react with oxidants.
: ;, . -;
It is therefore the aim of the invention to provide a process for the stabilization of chloramines.
Surprisingly, oxidants of the chloramine type, such a8 chloramine-T, chloramine-L or their salts or salts of HOCl in dried, preferably lyophilised form, are stabilized by reversibly dehydratable oxidation-resistant substances.
The invention thus relates to a process for the stabi-lization of chlor~mines or salts of hypochlorous acid in dried form, comprising the addition of an oxidation-resistant reversibly dehydratable substance.
Preferred substances are sugars, preferably maltose, or salts, preferably trisodium citrate dihydrate or calcium '.'':'~, 2~0~ Z
- 2 - ~:
acetate.
The substance 6uch as sugars, preferably maltose, or salts are employed in concentrations from 0.1-30, prefer-ably 1-5% (per cent by weight).
In lyophilised form, the oxidants stabilized in this way retain their oxidative strength even if the reagents are stored for more than two weeks at 37C.
The invention is further defined in the claims and is described in detail in the following example .
. . ''~ ``., '~
2(~ 4;~2 - 3 - :
Example Stabilization of chloramine by hygroscopic substances 450.7 mg chloramine-T (or 341.7 mq chloramine-B) were taken up in 20 ml of distilled water together with 94.3 mg of tranexamic acid and 600 mg in each case of - :~
maltose monohydrate, calcium acetate or trisodium citrate dihydrate and the pH-values were ad~usted to pH 10.
.: ::- ~:
After complete dissolution the solutions were used in the ~ehringwerke PAI-CAP test as a substitute for the "Oxidant" reagent. Additionally, the solutions were lyo-philised in portions of 1 ml and stored in solid (lyo-philised) form and in dissolved form at 37C and a PAI-CAP test was carried out with these reagents. Tris buffer or "Standard Sl" from the Behringwerke PAI-test kit was employed as the sample in each case. The results are given in the following table.
.: :: : . ~.-:, ,. .,: :,' .,. :' ..',,: ,., ::
. ,:: : . :. :. , ... ~:
...... ,.~,.. ~
,,;,"- ,,;~
, :, ~ " ;..-- - . . - , . .
. .
200~4~2 Table -chloramine-T chloramine-B .
measured plasmin act. (A/5 min).x 1000 :-buffer Standard Sl act. decrease in Std.
Sl respect.to buffer(%) ~:~
maltose immediate 1.298 1.210 7 10 after 1 week 1.271 1.076 15 23 after 2 weeks 1.078 0.909 16 25 .
calcium acetate immediate 1.262 1.156 8 15 ~ . :
after 1 week 1.212 1.051 13 23 .
after 2 weeks 1.160 1.002 14 22 trisodium citrate dihydrate immediate 1.363 1.205 12 15 --~
after 1 week 1.304 1.036 21 25 : .;~
after 2 weeks 1.190 0.971 18 20 : .
. . .
Mean values from two determinations ~ :~
Maltose, calcium acetate or trisodium citrate dihydrate are found to be almost inert towards oxidation with re-'' ''"'~,'!.~ "~`' spect to chloramines, which they stabilize.
. ,'. ','.' ~.,~
"
X
..: , - ..~, .....
,, ~. : , , ' '
Claims (5)
1. A process for the stabilization of chloramines or salts of hypochlorous acid in dried form, compris-ing the addition of an oxidation-resistant revers-ibly dehydratable substance.
2. The process as claimed in claim 1 wherein the sub-stance is maltose, calcium acetate or trisodium citrate dihydrate.
3. The process as claimed in claim 1, wherein the dried form is obtained by freeze-drying.
4. The process as claimed in at least one of claims 1 to 3, wherein the substances are employed in concentrations of 0.3-30 per cent by weight.
5. The process as claimed in claim 1, and substantially as described herein.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DEP3840815.5 | 1988-12-03 | ||
| DE19883840815 DE3840815A1 (en) | 1988-12-03 | 1988-12-03 | METHOD FOR STABILIZING CHLORAMINES OR SALTS OF LOWER CHLORINE ACID |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2004422A1 true CA2004422A1 (en) | 1990-06-03 |
Family
ID=6368421
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA 2004422 Abandoned CA2004422A1 (en) | 1988-12-03 | 1989-12-01 | Process for the stabilization of chloramines or salts of hypochlorous acid |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP0372416A1 (en) |
| JP (1) | JPH02209860A (en) |
| AU (1) | AU4572989A (en) |
| CA (1) | CA2004422A1 (en) |
| DE (1) | DE3840815A1 (en) |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE402983C (en) * | 1922-05-31 | 1924-09-19 | Chem Fab Von Heyden A G | Process for the preparation of dry, durable mixtures of salts of nitrogen-halogenated aromatic sulfonamides |
| US1784286A (en) * | 1927-04-15 | 1930-12-09 | C O Sethness | Process of making easily soluble stable hypochlorites |
| GB1304037A (en) * | 1970-04-23 | 1973-01-24 | ||
| US4124734A (en) * | 1976-04-30 | 1978-11-07 | Lever Brothers Company | Encapsulated particles |
-
1988
- 1988-12-03 DE DE19883840815 patent/DE3840815A1/en not_active Withdrawn
-
1989
- 1989-12-01 CA CA 2004422 patent/CA2004422A1/en not_active Abandoned
- 1989-12-01 EP EP89122201A patent/EP0372416A1/en not_active Withdrawn
- 1989-12-01 AU AU45729/89A patent/AU4572989A/en not_active Abandoned
- 1989-12-01 JP JP31082189A patent/JPH02209860A/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP0372416A1 (en) | 1990-06-13 |
| DE3840815A1 (en) | 1990-06-13 |
| AU4572989A (en) | 1990-06-07 |
| JPH02209860A (en) | 1990-08-21 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Dead |