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Genentech Inc
Original Assignee
Monsanto Co
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Publication date
Application filed by Monsanto CofiledCriticalMonsanto Co
Publication of CA2003361A1publicationCriticalpatent/CA2003361A1/en
Application grantedgrantedCritical
Publication of CA2003361CpublicationCriticalpatent/CA2003361C/en
Micro-Organisms Or Cultivation Processes Thereof
(AREA)
Preparation Of Compounds By Using Micro-Organisms
(AREA)
Peptides Or Proteins
(AREA)
Abstract
A process is disclosed for the production of a novel human vascular permeability factor in vitro comprising growing cells derived from the human histiocytic lymphoma cell line U-937 in nutrient culture medium at about 35° to 38°C for a sufficient time to elaborate vascular permeability factor and isolating the resulting vascular permeability factor from the spent cells or the cell culture conditioned medium.