CA1341531C - Cellules vegetales genetiquement modifiees, ainsi que des plants presentant une resistance aux inhibiteurs de la glutamine synthetase, et aussi des fragments d'adn et des recombines pour la production de ces cellules et de ces plantes - Google Patents
Cellules vegetales genetiquement modifiees, ainsi que des plants presentant une resistance aux inhibiteurs de la glutamine synthetase, et aussi des fragments d'adn et des recombines pour la production de ces cellules et de ces plantes Download PDFInfo
- Publication number
- CA1341531C CA1341531C CA000617061A CA617061A CA1341531C CA 1341531 C CA1341531 C CA 1341531C CA 000617061 A CA000617061 A CA 000617061A CA 617061 A CA617061 A CA 617061A CA 1341531 C CA1341531 C CA 1341531C
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- Prior art keywords
- val
- ala
- pro
- arg
- leu
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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- 239000012634 fragment Substances 0.000 title claims abstract description 124
- 102000005396 glutamine synthetase Human genes 0.000 title claims description 53
- 108020002326 glutamine synthetase Proteins 0.000 title claims description 53
- 238000004519 manufacturing process Methods 0.000 title description 5
- 230000001747 exhibiting effect Effects 0.000 title description 2
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 150
- 241000196324 Embryophyta Species 0.000 claims description 201
- 108020004414 DNA Proteins 0.000 claims description 97
- 210000004027 cell Anatomy 0.000 claims description 71
- 239000003112 inhibitor Substances 0.000 claims description 44
- 102000004169 proteins and genes Human genes 0.000 claims description 38
- 239000002773 nucleotide Substances 0.000 claims description 28
- 125000003729 nucleotide group Chemical group 0.000 claims description 28
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 26
- 230000000694 effects Effects 0.000 claims description 20
- 108020004705 Codon Proteins 0.000 claims description 17
- 230000009466 transformation Effects 0.000 claims description 15
- RLMISHABBKUNFO-WHFBIAKZSA-N Ala-Ala-Gly Chemical compound C[C@H](N)C(=O)N[C@@H](C)C(=O)NCC(O)=O RLMISHABBKUNFO-WHFBIAKZSA-N 0.000 claims description 14
- 150000001413 amino acids Chemical group 0.000 claims description 12
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 12
- 241000187747 Streptomyces Species 0.000 claims description 10
- 229920001184 polypeptide Polymers 0.000 claims description 10
- 108700039691 Genetic Promoter Regions Proteins 0.000 claims description 8
- 108020004511 Recombinant DNA Proteins 0.000 claims description 7
- 241000589158 Agrobacterium Species 0.000 claims description 6
- 108020002494 acetyltransferase Proteins 0.000 claims description 6
- 102000005421 acetyltransferase Human genes 0.000 claims description 6
- 241000701489 Cauliflower mosaic virus Species 0.000 claims description 5
- 108091026890 Coding region Proteins 0.000 claims description 5
- 108010003581 Ribulose-bisphosphate carboxylase Proteins 0.000 claims description 3
- FOWHQTWRLFTELJ-FXQIFTODSA-N Ala-Asp-Met Chemical compound C[C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CCSC)C(=O)O)N FOWHQTWRLFTELJ-FXQIFTODSA-N 0.000 claims description 2
- WOMUDRVDJMHTCV-DCAQKATOSA-N Glu-Arg-Arg Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O WOMUDRVDJMHTCV-DCAQKATOSA-N 0.000 claims description 2
- MXJYXYDREQWUMS-XKBZYTNZSA-N Glu-Thr-Ser Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CO)C(O)=O MXJYXYDREQWUMS-XKBZYTNZSA-N 0.000 claims description 2
- IAJOBQBIJHVGMQ-UHFFFAOYSA-N Phosphinothricin Natural products CP(O)(=O)CCC(N)C(O)=O IAJOBQBIJHVGMQ-UHFFFAOYSA-N 0.000 claims description 2
- BKIOKSLLAAZYTC-KKHAAJSZSA-N Thr-Val-Asn Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(N)=O)C(O)=O BKIOKSLLAAZYTC-KKHAAJSZSA-N 0.000 claims description 2
- 108010056243 alanylalanine Proteins 0.000 claims description 2
- 102000023732 binding proteins Human genes 0.000 claims description 2
- 108091008324 binding proteins Proteins 0.000 claims description 2
- 235000019804 chlorophyll Nutrition 0.000 claims description 2
- IAJOBQBIJHVGMQ-BYPYZUCNSA-N glufosinate-P Chemical compound CP(O)(=O)CC[C@H](N)C(O)=O IAJOBQBIJHVGMQ-BYPYZUCNSA-N 0.000 claims description 2
- 108010049041 glutamylalanine Proteins 0.000 claims description 2
- 230000008488 polyadenylation Effects 0.000 claims description 2
- 239000002243 precursor Substances 0.000 claims description 2
- 108010082527 phosphinothricin N-acetyltransferase Proteins 0.000 claims 6
- 108010050848 glycylleucine Proteins 0.000 claims 5
- SBHUBSDEZQFJHJ-CIUDSAMLSA-N Asp-Asp-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](N)CC(O)=O SBHUBSDEZQFJHJ-CIUDSAMLSA-N 0.000 claims 2
- FAUPLTGRUBTXNU-FXQIFTODSA-N Asp-Pro-Ser Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CO)C(O)=O FAUPLTGRUBTXNU-FXQIFTODSA-N 0.000 claims 2
- ITYRYNUZHPNCIK-GUBZILKMSA-N Glu-Ala-Leu Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(O)=O ITYRYNUZHPNCIK-GUBZILKMSA-N 0.000 claims 2
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- NZOAFWHVAFJERA-OALUTQOASA-N Gly-Phe-Trp Chemical compound [H]NCC(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CC1=CNC2=C1C=CC=C2)C(O)=O NZOAFWHVAFJERA-OALUTQOASA-N 0.000 claims 2
- JFFAPRNXXLRINI-NHCYSSNCSA-N His-Asp-Val Chemical compound [H]N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](C(C)C)C(O)=O JFFAPRNXXLRINI-NHCYSSNCSA-N 0.000 claims 2
- 241000880493 Leptailurus serval Species 0.000 claims 2
- RRVCZCNFXIFGRA-DCAQKATOSA-N Leu-Pro-Asn Chemical compound [H]N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC(N)=O)C(O)=O RRVCZCNFXIFGRA-DCAQKATOSA-N 0.000 claims 2
- SITLTJHOQZFJGG-UHFFFAOYSA-N N-L-alpha-glutamyl-L-valine Natural products CC(C)C(C(O)=O)NC(=O)C(N)CCC(O)=O SITLTJHOQZFJGG-UHFFFAOYSA-N 0.000 claims 2
- ICTZKEXYDDZZFP-SRVKXCTJSA-N Pro-Arg-Pro Chemical compound N([C@@H](CCCN=C(N)N)C(=O)N1[C@@H](CCC1)C(O)=O)C(=O)[C@@H]1CCCN1 ICTZKEXYDDZZFP-SRVKXCTJSA-N 0.000 claims 2
- BDMWLJLPPUCLNV-XGEHTFHBSA-N Ser-Thr-Val Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C(C)C)C(O)=O BDMWLJLPPUCLNV-XGEHTFHBSA-N 0.000 claims 2
- HSWXBJCBYSWBPT-GUBZILKMSA-N Ser-Val-Val Chemical compound CC(C)[C@H](NC(=O)[C@@H](NC(=O)[C@@H](N)CO)C(C)C)C(O)=O HSWXBJCBYSWBPT-GUBZILKMSA-N 0.000 claims 2
- FRMFMFNMGQGMNB-BVSLBCMMSA-N Tyr-Pro-Trp Chemical compound C([C@H](N)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(O)=O)C1=CC=C(O)C=C1 FRMFMFNMGQGMNB-BVSLBCMMSA-N 0.000 claims 2
- YFOCMOVJBQDBCE-NRPADANISA-N Val-Ala-Glu Chemical compound C[C@@H](C(=O)N[C@@H](CCC(=O)O)C(=O)O)NC(=O)[C@H](C(C)C)N YFOCMOVJBQDBCE-NRPADANISA-N 0.000 claims 2
- UKEVLVBHRKWECS-LSJOCFKGSA-N Val-Ile-Gly Chemical compound CC[C@H](C)[C@@H](C(=O)NCC(=O)O)NC(=O)[C@H](C(C)C)N UKEVLVBHRKWECS-LSJOCFKGSA-N 0.000 claims 2
- 108010045350 alanyl-tyrosyl-alanine Proteins 0.000 claims 2
- 108010069205 aspartyl-phenylalanine Proteins 0.000 claims 2
- 108010036413 histidylglycine Proteins 0.000 claims 2
- 108010057821 leucylproline Proteins 0.000 claims 2
- 108010080629 tryptophan-leucine Proteins 0.000 claims 2
- 241000589155 Agrobacterium tumefaciens Species 0.000 claims 1
- DVWVZSJAYIJZFI-FXQIFTODSA-N Ala-Arg-Asn Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(N)=O)C(O)=O DVWVZSJAYIJZFI-FXQIFTODSA-N 0.000 claims 1
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- MQIGTEQXYCRLGK-BQBZGAKWSA-N Ala-Gly-Pro Chemical compound C[C@H](N)C(=O)NCC(=O)N1CCC[C@H]1C(O)=O MQIGTEQXYCRLGK-BQBZGAKWSA-N 0.000 claims 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
- C12N15/8277—Phosphinotricin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
La présente invention a pour objet un fragment d'ADN contenant un gène déterminé, dont l'expression inhibe les effets antibiotiques et herbicides de Bialaphos et produits connexes. La présente invention concerne également des vecteurs recombinants contenant lesdits fragments d'ADN, qui permettent ce gène protecteur dêtre introduit et exprimé dans les cellules et les cellules végétales.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP87400141A EP0242236B2 (fr) | 1986-03-11 | 1987-01-21 | Cellules végétales résistantes aux inhibiteurs de la synthétase de glutamine, produites par génie génétique |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA000534044A Division CA1341117C (fr) | 1987-01-21 | 1987-04-07 | Plantes et cellules de plantes concues par voie genetique resistantes aux inhibiteurs de glutamine synthetase, fragments et recombinants d'adn utilises dans la production de ces plantes et cellules |
Publications (1)
Publication Number | Publication Date |
---|---|
CA1341531C true CA1341531C (fr) | 2007-06-12 |
Family
ID=33396155
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA000534044A Expired - Lifetime CA1341117C (fr) | 1987-01-21 | 1987-04-07 | Plantes et cellules de plantes concues par voie genetique resistantes aux inhibiteurs de glutamine synthetase, fragments et recombinants d'adn utilises dans la production de ces plantes et cellules |
CA000617061A Expired - Lifetime CA1341531C (fr) | 1987-01-21 | 1987-04-07 | Cellules vegetales genetiquement modifiees, ainsi que des plants presentant une resistance aux inhibiteurs de la glutamine synthetase, et aussi des fragments d'adn et des recombines pour la production de ces cellules et de ces plantes |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA000534044A Expired - Lifetime CA1341117C (fr) | 1987-01-21 | 1987-04-07 | Plantes et cellules de plantes concues par voie genetique resistantes aux inhibiteurs de glutamine synthetase, fragments et recombinants d'adn utilises dans la production de ces plantes et cellules |
Country Status (1)
Country | Link |
---|---|
CA (2) | CA1341117C (fr) |
-
1987
- 1987-04-07 CA CA000534044A patent/CA1341117C/fr not_active Expired - Lifetime
- 1987-04-07 CA CA000617061A patent/CA1341531C/fr not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
CA1341117C (fr) | 2000-10-17 |
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