CA1334182C - Tissue culture flask - Google Patents
Tissue culture flaskInfo
- Publication number
- CA1334182C CA1334182C CA000506835A CA506835A CA1334182C CA 1334182 C CA1334182 C CA 1334182C CA 000506835 A CA000506835 A CA 000506835A CA 506835 A CA506835 A CA 506835A CA 1334182 C CA1334182 C CA 1334182C
- Authority
- CA
- Canada
- Prior art keywords
- edge
- opening
- straight line
- given point
- main body
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
Landscapes
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
A tissue culture flask includes a container main body comprising a box having a bottom surface for supporting a substance to be cultured, a side surface, and an upper surface. A cylindrical neck is provided on the side surface of said container main body to communi-cate with the interior of said container main body, and extends obliquely upward with respect to the bottom sur-face and defining a first opening at a distal end thereof and a second opening at a proximal end thereof. In the flask, (1) a first angle defined by (a) a first straight line connecting a given point on the bottom surface in-cluding an edge thereof and a given point on an edge of the first opening and (b) a second straight line on the inner circumferential wall and connecting a given point on an edge of the first opening and a given point on an edge of the second opening is not greater than (2) a second angle defined by (c) said second straight line and (d) the longest third straight line connecting a given point on an edge of the first opening and a given point on an edge of the second opening.
Description
~ S34 1 82 The present invention relates to a tissue culture flask for tissue culture, from which a cultured tissue can be recovered by a rod-like recovering means.
Tissue culture flasks are used to culture cells, tissues, organs and so on in vitro. Tissue culture flasks which can be stacked and which are of as small a size as possible are required for convenience in maintenance.
This invention can be more fully understood from the following detailed description when taken in conjunction with the accompanying drawings, in which:
Figs. 1 to 3 show a structure of a conventional tissue culture flask, in which:
Fig. 1 is a left-side view; Fig. 2 is a bottom view;
and Fig. 3 is a front view.
Figs. 4 to 8 show an embodiment of the present invention, in which:
Fig. 4 is a plan view; Fig. 5 is a left-side view;
Fig. 6 is a sectional view taken along the line VI - VI of Fig. 4; Fig. 7 is a bottom view; and Fig. 8 is a front view.
Figs. 9 to 11 show another embodiment of the present invention, in which:
Fig. 9 is a left-side view; Fig. 10 is a bottom view;
and Fig. 11 is a front view.
Figs. 12 to 14 show another embodiment of the present invention, in which:
~., rm/
- la - 1 334 1 82 Fig. 12 is a left-side view; Fig. 13 is a bottom view; and Fig. 14 i~ a front view.
As shown in Figs. 1 to 3, conventional tissue culture flask 10 consists of container main body 2 and neck 3. Main body 2 is a box having flat bottom surface 21 and a substantially rectangular-parallelepiped interior. Front side surface 22 of main body 2 consists of inclined low sur-face 221 contiguous with surface 21, oblique side surfaces 222 and 223 contiguous with right-side surface 23 and left-side surface 24, respectively, and major surface 224 contiguous with surfaces 221, 222 and 223 and located at the front-most portion of main body 2. Neck 3 having container port 35 is integrally formed with surface 224 of surface 22 of main body 2 and extends obliquely upward with respect to surface 21.
In conventional flask 10, a distal end of pipette P or a scraper cannot reach the inner edge of surface 21 at a side of port 35 (see Fig. 2). Therefore, not all the cells attached to surface 21 in the inner surface of flask 10 can be recovered from port 35. That is, cells rm/, - in some area (hatched portion in Fig. 2) cannot be removed, resulting in inconvenience.
The present invention has been made in view of the above situation and has as its major object to provide a tissue culture flask having a shape that allows a distal end of a pipette or a scraper inserted from its container port to reach its entire bottom surface area.
The tissue culture flask of the present invention comprises a container main body comprising a box having a bottom surface for supporting a substance to be cultured, a front side surface, a rear side surface, a right side surface, a left side surface, and an upper surface, and a hollow cylindrical neck provided on the front side surface of the container main body to communicate with the interior of the container main body, extending obliquely upward with respect to the bottom surface and defining a first opening at a first end located away from the container main body and a second opening at an end opposite the first end. In the flask, a first angle is defined by a first straight line connecting any given point on an edge of the bottom surface and a given point on an edge of the first opening and a second straight line on an inner circumferential wall of the neck and connecting the given point on an edge of the first opening and a given point on an edge of the second opening. This first angle is not greater than a second angle defined by the second straight line and the longest third straight line connecting the given point on an edge of the first opening kb:ycc 2 13~4182 and a given point on an edge of the second opening. The bottom surface has a shape with n corners wherein n is an integer and n 2 5. The front side surface comprises n - 3 inclined lower surfaces, each being contiguous with a front edge of the bottom surface, two inclined side surfaces being contiguous with the right side surface and the left side surface, respectively, and an additional surface being contiguous with the neck.
Thus, a linear rod-like member inserted in the container main body from the neck can reach any point on the bottom surface of the container main body (including its edge).
The structure of the flask of the present invention will be described in detail with reference to the ycc/sp 3 accompanying drawings. 1 3~4 1 8 2 Figs. 4 to 8 show a first embodiment of the present nventlon .
Referring to Figs. 4 to 8, container main body 2 of tissue culture flask 1 consists of bottom surface 21, normally flat right- and left-side surfaces 23 and 24, upper surface 25, rear side surface 26 and front side surface 22. A side of surface 21 facing the interior of main body 2 is a flat surface.
A space at a rear portion of the container defined by surfaces 21, 23, 24, 25 and 26 preferably has a sub-stantially rectangular parallelpiped shape, as shown in the drawings. With this shape, flasks 1 can be stacked, can have a small size, and can minimize an error between a required amount of culture medium to be supplied and the amount of the medium actually supplied.
Surface 21 must be flat since it carries the culture medium contained in flask 1. Alternatively, surface 21 preferably has a polygonal shape defined by substantially linear lines with round corners. With such a structure, the area defined by surface 21 can be correctly determined, resulting in accurate culturing.
Since the rear portion of surface 21 has a -shape, surface 21 normally has five or more corners and is preferably a hexagon, as shown in Fig. 7.
Since bottom surface 21 of a conventional flask has a rectangular shape (see Fig. 2), a pipette inserted i3~4182 from container port 35 cannot reach either of the two front corners of surface 21. However, if the two cor-ners are cut so that surface 21 is a pentagon or a poly-gon having 5 or more corners (preferably a hexagon), when the interior of main body 2 is viewed from port 35 in varying directions by a human eye, all of the edges of surface 21 can be visually observed through port 35, thereby allowing the pipette or the like to easily reach all fillet portions.
When surface 21 is a polygon having five or more corners, front side surface 22 preferably consists of an oblique side surface, an inclined lower surface, and a major surface contiguous with neck 3, extending oblique-ly upward with respect to surface 22.
In Fig. 7, surface 21 is a hexagon. Accordingly, surface 22 consists of three inclined lower surfaces 225, 226 and 227 contiguous with front linear edge of surface 21, major surface 224, and with each other, and two oblique side surfaces 222 and 223. Surface 222 is contiguous with surfaces 225, 24, 21 and 224. Surface 223 is contiguous with surfaces 227, 23, 21 and 224.
In this case, it is apparent that inclined lower surfaces can be provided in accordance with the number of sides in the polygon formed by the bottom surface.
When the number of sides of the polygon is n (n _ 5), the number of the inclined lower surfaces is n - 3.
Cylindrical neck 3 having port 35 is integral with _ -- 6 1 3~4 1 82 surface 224 and extends obliquely upward with respect to surface 21. Thus, the area of surface 224 integral with neck 3 can be so reduced that it is negligible.
With this configuration, according to the first aspect of the present invention, when the interior of main body 2 is viewed from port 35, all the edges of bottom 21 can be seen directly. More specifically, re-ferring to Fig. 6, angle ~1 defined by straight line Ll connecting point Pl on a front end of an opening of cyl-indrical neck 3 and point P2 on a rear end of an opening thereof, and a circumferential wall surface of neck 3 is larger than angle ~2 defined by straight line L2 con-necting point Pl and given point P3 on an edge of sur-face 21 and the circumferential wall surface of neck 3.
This allows a rod-like recovery means to reach all of the edges of surface 21 with its distal end.
When legs 5 are provided to the bottom portion of flask 1, stability of flask 1 is increased.
Figs. 9 to 11 show another embodiment according to a second aspect of the present invention.
In Figs. 9 to 11, unlike the conventional flask shown in Figs. 1 to 3, curved portions 41 and 42 which project outward from main body 2 are provided on a con-tiguous portion of major surface 224 and neck 3.
Portions 41 and 42 are two separate curved surfaces or constitute a single curved portion obtained by con-necting two curved surfaces at a contiguous portion of surface 224 and neck 3 having port 35. With portions 41 and 42, the entire area of front corners of surface 21 can be seen directly through port 35, so that a linear pipette or the like can reach them.
Figs. 12 to 14 show still another embodiment accor-ding to a third aspect of the present invention obtained by combining the first and second aspects described above.
In Figs. 12 to 14, curved portions 41 and 42, which project outward from main body 2, are provided on a con-tiguous portion of surface 224 and neck 3 of the flask shown in Figs. 4 to 8.
When the second aspect is combined with the first aspect in this manner, a pipette or the like can reach any corner of the flask even if the inclinations of surfaces 225, 226 and 227 are steeper. Therefore, this enables size reduction and larger capacity in the tissue culture flasks.
The tissue culture flask of the present invention can be manufactured by forming a plurality of portions from a transparent material such as polystyrene, meth-acrylate resin, polycarbonate and polyethylene, and fus-ing them.
More particularly, a tissue culture flask of the present invention can be manufactured by separately forming a container main body including neck 3 (excluding upper surface 25), and upper surface 25, and fusing them.
In accordance with another manufacturing method, a tissue culture flask of the present invention can be manufactured by separately forming a container main body (excluding rear side surface 26 and containing neck 3 and surface 25), and rear side surface 26, and fusing them.
According to the present invention, a pipette or the like can reach all the edges of the bottom surface of the tissue culture flask, so that a recovery effi-ciency of cells and so on attached to the bottom of the flask can be greatly increased.
According to the first aspect of the present inven-tion, the flasks can be stacked, the overall size is compact, and the required supply amount of culture me-dium can be easily discriminated from the depth of the culture liquid actually supplied.
According to the second aspect of the present in-vention, the area of the bottom surface of the flask can be determined correctly, and culturing, handling of the flask for analysis, and manufacture of the flask are easy.
According to the third aspect of the present invention, the effect of the present invention can be obtained by only slightly modifying the shape of a conventional tissue culture flask. Thereby, the amount of culture medium that is not removed upon decantation of the culture is decreased. When the third aspect of the present invention is combined with the second aspect, the inclination of the inclined lower surface of the front side surface can be increased. As a result, reduction in size and larger capacity of the flask and a decrease in the number of cells attached to the inclined surface thereof can be enabled, and accidental out flow of the culture medium due to vibration can be prevented.
Tissue culture flasks are used to culture cells, tissues, organs and so on in vitro. Tissue culture flasks which can be stacked and which are of as small a size as possible are required for convenience in maintenance.
This invention can be more fully understood from the following detailed description when taken in conjunction with the accompanying drawings, in which:
Figs. 1 to 3 show a structure of a conventional tissue culture flask, in which:
Fig. 1 is a left-side view; Fig. 2 is a bottom view;
and Fig. 3 is a front view.
Figs. 4 to 8 show an embodiment of the present invention, in which:
Fig. 4 is a plan view; Fig. 5 is a left-side view;
Fig. 6 is a sectional view taken along the line VI - VI of Fig. 4; Fig. 7 is a bottom view; and Fig. 8 is a front view.
Figs. 9 to 11 show another embodiment of the present invention, in which:
Fig. 9 is a left-side view; Fig. 10 is a bottom view;
and Fig. 11 is a front view.
Figs. 12 to 14 show another embodiment of the present invention, in which:
~., rm/
- la - 1 334 1 82 Fig. 12 is a left-side view; Fig. 13 is a bottom view; and Fig. 14 i~ a front view.
As shown in Figs. 1 to 3, conventional tissue culture flask 10 consists of container main body 2 and neck 3. Main body 2 is a box having flat bottom surface 21 and a substantially rectangular-parallelepiped interior. Front side surface 22 of main body 2 consists of inclined low sur-face 221 contiguous with surface 21, oblique side surfaces 222 and 223 contiguous with right-side surface 23 and left-side surface 24, respectively, and major surface 224 contiguous with surfaces 221, 222 and 223 and located at the front-most portion of main body 2. Neck 3 having container port 35 is integrally formed with surface 224 of surface 22 of main body 2 and extends obliquely upward with respect to surface 21.
In conventional flask 10, a distal end of pipette P or a scraper cannot reach the inner edge of surface 21 at a side of port 35 (see Fig. 2). Therefore, not all the cells attached to surface 21 in the inner surface of flask 10 can be recovered from port 35. That is, cells rm/, - in some area (hatched portion in Fig. 2) cannot be removed, resulting in inconvenience.
The present invention has been made in view of the above situation and has as its major object to provide a tissue culture flask having a shape that allows a distal end of a pipette or a scraper inserted from its container port to reach its entire bottom surface area.
The tissue culture flask of the present invention comprises a container main body comprising a box having a bottom surface for supporting a substance to be cultured, a front side surface, a rear side surface, a right side surface, a left side surface, and an upper surface, and a hollow cylindrical neck provided on the front side surface of the container main body to communicate with the interior of the container main body, extending obliquely upward with respect to the bottom surface and defining a first opening at a first end located away from the container main body and a second opening at an end opposite the first end. In the flask, a first angle is defined by a first straight line connecting any given point on an edge of the bottom surface and a given point on an edge of the first opening and a second straight line on an inner circumferential wall of the neck and connecting the given point on an edge of the first opening and a given point on an edge of the second opening. This first angle is not greater than a second angle defined by the second straight line and the longest third straight line connecting the given point on an edge of the first opening kb:ycc 2 13~4182 and a given point on an edge of the second opening. The bottom surface has a shape with n corners wherein n is an integer and n 2 5. The front side surface comprises n - 3 inclined lower surfaces, each being contiguous with a front edge of the bottom surface, two inclined side surfaces being contiguous with the right side surface and the left side surface, respectively, and an additional surface being contiguous with the neck.
Thus, a linear rod-like member inserted in the container main body from the neck can reach any point on the bottom surface of the container main body (including its edge).
The structure of the flask of the present invention will be described in detail with reference to the ycc/sp 3 accompanying drawings. 1 3~4 1 8 2 Figs. 4 to 8 show a first embodiment of the present nventlon .
Referring to Figs. 4 to 8, container main body 2 of tissue culture flask 1 consists of bottom surface 21, normally flat right- and left-side surfaces 23 and 24, upper surface 25, rear side surface 26 and front side surface 22. A side of surface 21 facing the interior of main body 2 is a flat surface.
A space at a rear portion of the container defined by surfaces 21, 23, 24, 25 and 26 preferably has a sub-stantially rectangular parallelpiped shape, as shown in the drawings. With this shape, flasks 1 can be stacked, can have a small size, and can minimize an error between a required amount of culture medium to be supplied and the amount of the medium actually supplied.
Surface 21 must be flat since it carries the culture medium contained in flask 1. Alternatively, surface 21 preferably has a polygonal shape defined by substantially linear lines with round corners. With such a structure, the area defined by surface 21 can be correctly determined, resulting in accurate culturing.
Since the rear portion of surface 21 has a -shape, surface 21 normally has five or more corners and is preferably a hexagon, as shown in Fig. 7.
Since bottom surface 21 of a conventional flask has a rectangular shape (see Fig. 2), a pipette inserted i3~4182 from container port 35 cannot reach either of the two front corners of surface 21. However, if the two cor-ners are cut so that surface 21 is a pentagon or a poly-gon having 5 or more corners (preferably a hexagon), when the interior of main body 2 is viewed from port 35 in varying directions by a human eye, all of the edges of surface 21 can be visually observed through port 35, thereby allowing the pipette or the like to easily reach all fillet portions.
When surface 21 is a polygon having five or more corners, front side surface 22 preferably consists of an oblique side surface, an inclined lower surface, and a major surface contiguous with neck 3, extending oblique-ly upward with respect to surface 22.
In Fig. 7, surface 21 is a hexagon. Accordingly, surface 22 consists of three inclined lower surfaces 225, 226 and 227 contiguous with front linear edge of surface 21, major surface 224, and with each other, and two oblique side surfaces 222 and 223. Surface 222 is contiguous with surfaces 225, 24, 21 and 224. Surface 223 is contiguous with surfaces 227, 23, 21 and 224.
In this case, it is apparent that inclined lower surfaces can be provided in accordance with the number of sides in the polygon formed by the bottom surface.
When the number of sides of the polygon is n (n _ 5), the number of the inclined lower surfaces is n - 3.
Cylindrical neck 3 having port 35 is integral with _ -- 6 1 3~4 1 82 surface 224 and extends obliquely upward with respect to surface 21. Thus, the area of surface 224 integral with neck 3 can be so reduced that it is negligible.
With this configuration, according to the first aspect of the present invention, when the interior of main body 2 is viewed from port 35, all the edges of bottom 21 can be seen directly. More specifically, re-ferring to Fig. 6, angle ~1 defined by straight line Ll connecting point Pl on a front end of an opening of cyl-indrical neck 3 and point P2 on a rear end of an opening thereof, and a circumferential wall surface of neck 3 is larger than angle ~2 defined by straight line L2 con-necting point Pl and given point P3 on an edge of sur-face 21 and the circumferential wall surface of neck 3.
This allows a rod-like recovery means to reach all of the edges of surface 21 with its distal end.
When legs 5 are provided to the bottom portion of flask 1, stability of flask 1 is increased.
Figs. 9 to 11 show another embodiment according to a second aspect of the present invention.
In Figs. 9 to 11, unlike the conventional flask shown in Figs. 1 to 3, curved portions 41 and 42 which project outward from main body 2 are provided on a con-tiguous portion of major surface 224 and neck 3.
Portions 41 and 42 are two separate curved surfaces or constitute a single curved portion obtained by con-necting two curved surfaces at a contiguous portion of surface 224 and neck 3 having port 35. With portions 41 and 42, the entire area of front corners of surface 21 can be seen directly through port 35, so that a linear pipette or the like can reach them.
Figs. 12 to 14 show still another embodiment accor-ding to a third aspect of the present invention obtained by combining the first and second aspects described above.
In Figs. 12 to 14, curved portions 41 and 42, which project outward from main body 2, are provided on a con-tiguous portion of surface 224 and neck 3 of the flask shown in Figs. 4 to 8.
When the second aspect is combined with the first aspect in this manner, a pipette or the like can reach any corner of the flask even if the inclinations of surfaces 225, 226 and 227 are steeper. Therefore, this enables size reduction and larger capacity in the tissue culture flasks.
The tissue culture flask of the present invention can be manufactured by forming a plurality of portions from a transparent material such as polystyrene, meth-acrylate resin, polycarbonate and polyethylene, and fus-ing them.
More particularly, a tissue culture flask of the present invention can be manufactured by separately forming a container main body including neck 3 (excluding upper surface 25), and upper surface 25, and fusing them.
In accordance with another manufacturing method, a tissue culture flask of the present invention can be manufactured by separately forming a container main body (excluding rear side surface 26 and containing neck 3 and surface 25), and rear side surface 26, and fusing them.
According to the present invention, a pipette or the like can reach all the edges of the bottom surface of the tissue culture flask, so that a recovery effi-ciency of cells and so on attached to the bottom of the flask can be greatly increased.
According to the first aspect of the present inven-tion, the flasks can be stacked, the overall size is compact, and the required supply amount of culture me-dium can be easily discriminated from the depth of the culture liquid actually supplied.
According to the second aspect of the present in-vention, the area of the bottom surface of the flask can be determined correctly, and culturing, handling of the flask for analysis, and manufacture of the flask are easy.
According to the third aspect of the present invention, the effect of the present invention can be obtained by only slightly modifying the shape of a conventional tissue culture flask. Thereby, the amount of culture medium that is not removed upon decantation of the culture is decreased. When the third aspect of the present invention is combined with the second aspect, the inclination of the inclined lower surface of the front side surface can be increased. As a result, reduction in size and larger capacity of the flask and a decrease in the number of cells attached to the inclined surface thereof can be enabled, and accidental out flow of the culture medium due to vibration can be prevented.
Claims (4)
1. A tissue culture flask comprising:
a container main body comprising a box having a bottom surface for supporting a substance to be cultured, a front side surface, a rear side surface, a right side surface, a left side surface, and an upper surface; and a cylindrical neck provided on the front side surface of said container main body to communicate with the interior of said container main body, extending obliquely upward with respect to the bottom surface and defining a first opening at a first end located away from said container main body and a second opening at an end opposite said first end, wherein (1) a first angle defined by (a) a first straight line connecting any given point on an edge of the bottom surface and a given point on an edge of the first opening and (b) a second straight line on an inner circumferential wall of the neck and connecting said given point on the edge of the first opening and a given point on an edge of the second opening is not greater than (2) a second angle defined by (c) said second straight line and (d) the longest third straight line connecting said given point on the edge of the first opening and a given point on the edge of the second opening, and wherein the bottom surface has a shape with n corners wherein n is an integer and n ? 5, and the front side surface comprises n - 3 inclined lower surfaces, each contiguous with a front edge of the bottom surface, two inclined side surfaces contiguous with the right-side surface and the left-side surface, respectively, and an additional surface contiguous with the neck.
a container main body comprising a box having a bottom surface for supporting a substance to be cultured, a front side surface, a rear side surface, a right side surface, a left side surface, and an upper surface; and a cylindrical neck provided on the front side surface of said container main body to communicate with the interior of said container main body, extending obliquely upward with respect to the bottom surface and defining a first opening at a first end located away from said container main body and a second opening at an end opposite said first end, wherein (1) a first angle defined by (a) a first straight line connecting any given point on an edge of the bottom surface and a given point on an edge of the first opening and (b) a second straight line on an inner circumferential wall of the neck and connecting said given point on the edge of the first opening and a given point on an edge of the second opening is not greater than (2) a second angle defined by (c) said second straight line and (d) the longest third straight line connecting said given point on the edge of the first opening and a given point on the edge of the second opening, and wherein the bottom surface has a shape with n corners wherein n is an integer and n ? 5, and the front side surface comprises n - 3 inclined lower surfaces, each contiguous with a front edge of the bottom surface, two inclined side surfaces contiguous with the right-side surface and the left-side surface, respectively, and an additional surface contiguous with the neck.
2. A flask according to claim 1, wherein an inner space of the flask defined by the bottom surface, the upper surface, the rear side surface, the right-side surface, the left-side surface and a plane that contains said edge of said bottom surface comprises a substantially rectangular parallelepiped.
3. A flask according to claim 1 or claim 2, wherein the number of corners n = 6.
4. A tissue culture flask comprising:
a container main body comprising a box having a planar polygonal bottom surface for supporting a substance to be cultured, a front side surface, a rear side surface positioned opposite said front side surface, and an upper surface, said bottom surface having a front edge positioned opposite said rear side surface; and a cylindrical neck provided on the front side surface of said container main body to communicate with the interior of said container main body, extending obliquely upward with respect to the bottom surface and defining a first opening at a first end located away from said container main body and a second opening at an end opposite said first end, wherein a first straight line comprises a straight line connecting any given point on the front edge of the bottom surface and a given point on an edge of the first opening, a second straight line comprises a straight line on an inner circumferential wall of the neck and connecting said given point on the edge of the first opening and a given point on an edge of the second opening, a third straight line comprises the longest straight line connecting said given point on the edge of the first opening and a given point on an edge of the second opening, a first angle defined by the intersection of said first straight line and said second straight line is not greater than a second angle defined by the intersection of said second straight line and said third straight line.
a container main body comprising a box having a planar polygonal bottom surface for supporting a substance to be cultured, a front side surface, a rear side surface positioned opposite said front side surface, and an upper surface, said bottom surface having a front edge positioned opposite said rear side surface; and a cylindrical neck provided on the front side surface of said container main body to communicate with the interior of said container main body, extending obliquely upward with respect to the bottom surface and defining a first opening at a first end located away from said container main body and a second opening at an end opposite said first end, wherein a first straight line comprises a straight line connecting any given point on the front edge of the bottom surface and a given point on an edge of the first opening, a second straight line comprises a straight line on an inner circumferential wall of the neck and connecting said given point on the edge of the first opening and a given point on an edge of the second opening, a third straight line comprises the longest straight line connecting said given point on the edge of the first opening and a given point on an edge of the second opening, a first angle defined by the intersection of said first straight line and said second straight line is not greater than a second angle defined by the intersection of said second straight line and said third straight line.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP58170891A JPS6070067A (en) | 1983-09-16 | 1983-09-16 | Flask for tissue culture |
| CA000506835A CA1334182C (en) | 1983-09-16 | 1986-04-16 | Tissue culture flask |
| US07/728,048 US5139952A (en) | 1983-09-16 | 1991-07-08 | Tissue culture flask |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP58170891A JPS6070067A (en) | 1983-09-16 | 1983-09-16 | Flask for tissue culture |
| CA000506835A CA1334182C (en) | 1983-09-16 | 1986-04-16 | Tissue culture flask |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1334182C true CA1334182C (en) | 1995-01-31 |
Family
ID=25670959
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000506835A Expired - Fee Related CA1334182C (en) | 1983-09-16 | 1986-04-16 | Tissue culture flask |
Country Status (2)
| Country | Link |
|---|---|
| JP (1) | JPS6070067A (en) |
| CA (1) | CA1334182C (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5139952A (en) * | 1983-09-16 | 1992-08-18 | Terumo Corporation | Tissue culture flask |
| EP0141104A1 (en) * | 1983-09-28 | 1985-05-15 | Becton Dickinson and Company | Culture Flask |
| US4770854A (en) * | 1986-02-03 | 1988-09-13 | Costar Corporation | Laboratory flask |
| CA2175626C (en) * | 1995-05-18 | 1999-08-03 | Timothy A. Stevens | Tissue culture flask |
| JP5810850B2 (en) * | 2011-11-08 | 2015-11-11 | 大日本印刷株式会社 | Resin cell culture vessel and method for producing the same |
| WO2016103485A1 (en) * | 2014-12-26 | 2016-06-30 | 株式会社サンプラテック | Culture flask |
| WO2019210063A1 (en) * | 2018-04-25 | 2019-10-31 | Ge Healthcare Bio-Sciences Corp. | Bioreactors |
-
1983
- 1983-09-16 JP JP58170891A patent/JPS6070067A/en active Granted
-
1986
- 1986-04-16 CA CA000506835A patent/CA1334182C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6070067A (en) | 1985-04-20 |
| JPH0218061B2 (en) | 1990-04-24 |
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