CA1316479C - Process for the production of vaccine for prevention of pasteurella haemolytica pneumonia in bovine - Google Patents

Abstract

A serum-free vaccine effective against pneumonic pasteurellosis in cattle comprising a non-toxic leukotoxin specific for ruminant leukocytes is disclosed.
The leukotoxin is prepared in a serum-free medium from a culture of Pasteurella haemolytica. The produced leukotoxin is harvested from the culture medium upon detecting a certain stage during the logarithmic phase of the cell growth to obtain the optimum concentration of produced cytotoxin in the serum-free medium. Cattle may be treated with the vaccine to develop anti-leukotoxic immunity to pneumonic pasterellosis.

Description

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FIELD OF THE INVENTION
This invention relates to vaccines and processes for making same which are effective against a type of pneumonia i~ animals.

_ _ _ In the raising of animals for commercial purposes, various strains of pneumonia causing organisms can be a siynificant cause of animal death. More particularly in the raising of cattle, "Shipping Feverl' pneumonia is the major cause of sickness and mortality in feedlot cattle in North America. Although several respiratory viruses and bacteria have been implicated in the pathogenesis of the syndrome, the principal well known organism isolated is Pasteurella haem_lytica serotype Al. The disease can be reproduced experimentally by intratrachael inoculation of the microorganism. Bacterins incorporating P. haemolytica have been in use for more than sixty years in preventing this disease without significant impact on disease control. Evidence from field studies and experimental trials suggests an adverse effect of vaccination using the bacterins.
~nimals vaccinated with inactivated whole cell bac~erins frequently show a higher incidence of pneumonia and more severe lesions at post mortem than do unvaccinated animals. This occurs despite the induction of serum antibody to P. haemolytica cell surface antigens, measured by bacterial ag~lutination or passive hemaglutination techniques. This has resulted in considerable confusion with respect to how this type of 30 pneumonia can be prevented. Paradoxically, the occurrence of an analogous response as a result of natural or experimental infection with live bacteria has resulted in developing a degree of immunity -to pneumonia in so infected animals.
It has been determined that culture supernatant of Pasteurella haemolytica is cytotoxic to bovine but not porcine cells, as reported in "Cytotoxin of Pasteurella haemolytica Acting on Bovine Leukocytes", P.E. Shewen and B.N. Wilkie, Infection and Immunity, Jan. 1982, Vol.
35 No. 1, 91. Work was then directed to the production 2 ~31~79 of the cytotoxin by culture of Pasteurella haemolytica and the conversion of culture supernatant into a vaccine. The impetus for development of such a vaccine partially resulted from the generation of anti-toxic immune response after natural exposure of animals to P.
haemolytica. Calves vaccinated with leukotoxic culture supernate isolated from the culture of P. haemolytica produced both anti-toxic and bacterial agglutinating antibody. The so vaccinated calves were more resistant to experimental challenge than were counterparts vaccinated with bacterins or unvaccinated calves, as reported in "Immunity to Pasteurella haemolytica Cytotoxin", P.E. Shewen and B.N. Wilkie, 1982, Conf.
Res. Workers Animal Disease, Chicago, Illinois, Abstract 138.
As a result, production ln vitro of the cytotoxin by Pasteurella haemolytica has become very much of __ interest in an attempt to make a suitable vaccine on a commercial ba~sis for counteracting "Shipping Fever"
pneumonia. To date, the only viable technique for the ln vitro production of cytotoxin has required the addition of serum or blood to the culture medium and in particular the use of fetal calf sexum. Any attempt to manufacture the cytotoxin in a serum-free medium by culturing P. haemol~tica has resulted in what was thought to be an absence of produced cytotoxin because any assay for the cytotoxin was negative. Fetal calf serum is used as a seven percent solution which has been established to be the minimum amount needed to permit production of toxic culture supernate in RPMI 1640 medium. With the use of fetal calf serum or other stabilizing serum, heat-labile leukotoxin is made by culturing the P. haemolytica and harvesting the cytotoxic supe`rnatant after approximately one hour of growth at 37C. in the manner reported in the aforementioned article "Cytotoxin o Pasteurella haemolytica Acting on Bovine Leukocytes". The use of serum and particularly fetal calf serum in the manufacture of the cytotoxin complicates analysis of P.

, ~L3~6~79 haemolytica antigens present in culture supernate, greatly increases the cost for vaccine production and introduces potentially harm~ul extraneous antigens into the vaccine preparations. Furthermore, the presence of the serum in the supernate maintains activity of the toxin, which is undesirable in the vaccina preparation.
SUMMARY OF THE INVENTION
According to an aspect of this invention, a serum-free medium containing the cytotoxin to leukocytes is prepared from a serum-free culture of Pasteurella haemolytica. The process comprises culturing Pasteurella haemolytica viable cells in a serum-free medium to produce the cytotoxin. A determinant of logarithmic phase growth of the viable cells is monitored. A liquid containing the cytotoxin is harvested from the medium upon detecting a predetermined characteristic of the determinant in the monitored logarithmic phase of cell growth. The predetermined characteristic corresponds to an optimum concentration of produced cytotoxin in the serum-free medium.
According to another aspect of the invention, a process for producing a non-toxic inactive cytotoxin in a serum-free medium from a culture of Pasteurella haemolytica Al and removing the cytotoxin produced therefrom, comprises:
(A) culturing Pasteurella haemolytica viable cells in a serum-free medium to produce the cytotoxin, (B) monitoring a determinant of logarithmic phase growth of the viable cells, (C) upon detecting a pred~termined characteristic of the determinant which corresponds to an optimum concentration of cytotoxin produced in the serum-free medium, harvesting a liquid containin~ the cytotoxin from the medium; and ~D) separating solids including any of the cells to provide a Pasteurella haemolytica cell-free solution of the cytotoxin.

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3a 1316 ~79 According to another aspect of the invention, the liquid containing the cytotoxin may be converted into an animal vaccine.
According to another aspect of the invention, a vaccine effective against pneumonic pasteurellosis in cattle comprises a serum-free medium containing an inactive leukotoxin specific for ruminant leukocytes.
According to another aspect of the invention, a method for treating cattle to develop anti-leukotoxic immunity to pneumonic pasteurellosis comprises administering to cattle an effective protective amount of the serum-free vaccine.
BRIEF DESCRIPTION OF THE DR WINGS
Preferred embodiments of the invention are shown in the drawings wherein:
Figure 1 is a graph showing the relationship of leukotoxic production to the growth curve of P.
haemolYtica in serum-free medium where 0 is -.. . .

~3~6i.~9 growth curve, logl0 CFU per ml; ~ - ~ is total toxicity in culture supernate; and O _ ~
i5 heat-labile toxicity in culture supernate; and Figure 2 is a ~raph showing the relationsllip between the growth curve of P. haemolytica in serum~free medium and the optical density oE the culture at 525 nm, where ~ is growth curve, logl0 CFU per ml; and - ~ is optical density at 525nm.
DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS
7 . = _~
Although the mechanism is not fully understood as to the manner in which infection of cattle with Pasteurella haemolytica results in pneumonia and other infections such as mastitis in milking cows, it is realized that the preparation of vaccines based on P.
haemolytica cells are inefficatious. Surprisingly, cattle vaccinated with vaccines based on bacterins have increased susceptability to the "Shipping Fever"
pneumonia. In addition, immediate anaphylactoid reactions occur. Furthermore, the use of live bacteria as vaccines produce severe local reactions at injection sites and in common with live vaccines are problematic with respect to production, storage, distribution and use. However, a vaccine based on a cytotoxin prepared by culturin~ P. haemolytlca has been shown to protect or prevent pneumonic pasteurellosis of cattle. It is thought that the cytotoxin, which is a leukotoxin specific for ruminant leukocytes, is an important virulence factor in the induction of pasteurella pneumonia. The use of fetal calf serum in existing processes for the production of cytotoxin and resultant conversion into vaccine was thought to be necessary because culturing _ haemolytica on a serum-free medium to produce cytotoxin did not result in the detectable presence of active cytotoxin in the culture supernate.
According to a preferred embodiment of this invention, Pasteruella haemolytica may ~e grown in a serum-free medium, such as RPMI 1640 medium which is available from GIBCO, Grand Island, New York. It has heen discovered that the culture of P. haemolytica in a :~3~79 serum-free medium, such as RPMI 1640, produces the cytotoxin. However, it has been discovered that continued culture of P. haemolytica results in a disappearance of the cytotoxin either by loss of its toxicity or degradation thereof. It was discovered that the cytotoxin can be harvested from the culture medium of P. haemolytica in a serum-free medium at an appropriate time interval to optimize on the concentration of usable cytotoxin present in the medium.
To determine the time period when to harvest the liquid containing the cytotoxin from the medium, the toxicity of the supernate of the culture medium was investigated over extended periods of culturing of the P. haemolytica to develop a relationship of leukotoxin production compared to the growth curve of P~ haemolytica in the serum-free medium. With reference to Figure 1, the growth curve for _ haemolytica is represented on the scale ''Log10 CFU per ml", where CFU represents colony-forming units. For extended incubation times in the range of 350 minutes, periodically supernatant was isolated and the toxicity of the cytotoxin in the supernate was analy~ed. The total toxicity in culture supernate, along with the heat-labile activity of the cytotoxin in the culture supernate, were s~own to rise rapidly with the logarithmic phase growth of the P.
haemoly_ica cells and then commence falling off after incubation times greater than approximately 15a to 200 minutes insofar as the particular example shown in Figure 1.
It becomes apparent from Figure 1 that the optimum condition for harvesting the supernate is when cytotoxin is at its highest concentration, and as shown in Figure 1, this is when the culture is in logarithmic ~rowth phase. Therefore, in the culturing of P. haemolytica in the serum-free medium, a determinate of the logarithmic phase growth of viable cells has to be monitored to indicate when it is best to harvest the cytotoxin containing liquid. According to a preferred embodiment of this invention, the determinate of the logarithmic 6 13~6~7~

phase growth of the cells is the optical density of the culture medium.
With reEerence to Figure 2, the relationship between the growth curve of the P. haemolytica similar S to that of ~igure 1 is plotted with respect to optical density o~ the culture medium measured at a wavelength of 525 nm. On the basis of the results plotted in Figure 1, an approximate tenfold increase in the colony forming units (CFU3 per ml. indicates the time during which cytotoxin should be harvested from the medium.
This corresponds with a change in optical density ranging from approximately 0.18 up to approximately 0.37 (Figure 2). This corresponds with an incubation period of approximately 1.5 to 3 hours for the P. haemolytica in a serum-free medium such as RPMI 1640.
As illustrated in Figure 1, bacterial growth commenced immediately upon inoculation of P. haemolytica into the serum-free medium RPMI 1640 without an appreciable lag phase. Detectable heat-labile toxic activity in culture supernate increased during early logarithmic growth, maintained a plateau in the late logarithmic stage and declined in stationary phase culture~ From this growth curve, it was determined that the peak production of heat-labile toxin was achieved when bacteria had undergone a terlfold increase in CFU
which corresponds with the already noted change in optical density. When the supernate is harvested at the optimum time, which has been estahlished as the predetermined characteristic of optical density of approximately 0.37, it was also discovered that measurable toxicity of the harvested cytotoxin could not be evaluated unless fetal calf serum was added to the supernate to stabilize the toxicity of the cytotoxin.
Therefore, in evaluating the toxicity of the cytotoxin, it was necessary to stabilize each supernate isolated from the cuIture at the times shown in Figure 1 so that its toxicity could be evaluated. Alternatively, the culture supernate can be frozen immediately on isolation at -70C to retain toxicity without the addition of 7 :~3t~7~

serum. The frozen supernate is retained at that temperature until analysis is conducted to determine the toxicity of the supernate at that particular time of isolation during the culture of P. haemol~tica.
According to this invention, a process is provided for making the desired cytotoxin in a serum-free medium which provides a distinct advantage over the prior processes, wherein it was thought necessary to use fetal calf serum or other stabilizing serum in the medium to permit toxi~enic growth of the bacteria in producing the desired cytotoxin. ~s is established with reference to the results in Figure 1, it is only necessary to add serum to the supernate after isolation from the culture medium, i.e. at harvest, to stabili2e and maintain the toxic activity of the desired cytotoxin. This is, of course, only necessary when it is desired to assay for the presence of cytotoxin in the supernate.
Alternatively, the supernate can be frozen and maintained at -70C to retain toxicity of the cytotoxin.
Otherwise, without the addition of serum, activity of the cytotoxin is rapidly lost in the supernate. This is desirable in the manufacture of vaccine, because it provides a non-toxic inactive cytotoxin in the vaccine medium which is not harmful to the recipient yet as discovered, the inactive toxin retains the ability to elicit an immune response in the animal.
The optimum time to harvest cultured supernate in isolating the cytotoxin for vaccine is during early to mid logarithmic growth of the P. haemolytica~ It has been found that growth in the serum~free medium is sufficiently variable that a characteristic of a determinate of the growth~of the bacterium must be monitored rather than specifying the specific time during growth of the bacterium at which harvest is to take place. A useful determinant, according to this preferred embodiment, is optical density where a change in optical density at 525 nm. from approximately 0.18 at commencement of culturing of a concentration of cells of appro~imately 107 CFU per ml to 0.37 which coxresponds 8 ~ 3~79 to approximately 10 CFU per ml. The correspondin~
duration of time needed for this tenfold increase in cell growth has been found to vary from 1.5 up to 3 hours for P. haemolytica Al.
-After harvesting the supernate liquid containing the cytotoxin from the medium, a suitable vaccine may be prepared from the harvested liquid. A sample of the harvested liquid may be stabilized with fetal calf serum and assay conducted to determine and confirm toxicity of the produced cytotoxin. At harvest, the liquid is processed to remove extraneous matter. For example, the harvested liquid may be centrifuged and filtered to remove all solids which include cells, cell wall fra~ments~ unwanted metabolites and the like, thereby providing a liquid which is cell free and which is relatively endotoxin free. Thus when the vaccine is administered, the likelihood of anaphylactoid reactions is minimized which is a problem with prior vaccines of this nature due to the presence of endotoxin in the cell wall of the gram negative bacter:ium P. haemol~tica. ~t is appreciated that a variety of suitable techniques are avialable ~or isolating the cytotoxin and preparing the vaccine which are readily known to those skilled in the art. The selection of suitable techniques is primarily determined by the product to be prepared and the scale of commercialization.
The purified liquid is then treated in accordance with standard procedures in preparing a vaccine. The liquid is lyophilized to produce a stable composition when reconstituted in saline to the appropriate concentration for administration to animals.
preferred concentration is in the range of at least threefold. Various expedients may be added to the vaccine to improve its efficiency. Thus well known adjuvants may be added to the vaccine to optimize in the protection against pneumonic pasteurellosis in animals.
Preferred aspects of the invention are set out in the following Examples.

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Pasteurella haemolytica Culture and Leukotoxin Production .. .. .. _ _ _ Several colonies from an 18-hour blood agar plate of _ haemolytica type Al were inoculated into 500 ml of brain-heart infusion broth in each of four 1 liter Erlenmyer flasks and grown for 4.5 hours at 37C on a rocking platform. During this period, the cultures were in the early logarithmic phase of growth. Bacteria were pelleted by centrifugation at 4,000 x g for 10 minutes, pooled, and suspended to a concentration of approximately 10 colony-forming units tCFU)/ml. This concentration was estimated spectrophotometrically. The cells were suspended in 1 liter of RPMI 1640 medium which is readily available from GIBCO, Grand Island, New York. The medium was placed in a 2 liter Erlenmeyer flask and incubated at 37C on a rocking platform.
Before commencing of this incubation (time 0) and at specified time intervals thereafter, in the manner illustrated in Figure 1, 6 ml samples were periodically removed aseptically from the culture and assayed as follows. The optical density was read at 525 nm. and the number of the CE'U per milliliter was determined using a standard plate-count technique. After centrifugation at 6,000 x g for 15 minutes, the supernate was filtered through a 0.22 um filter available from Millipore Corp., of Bedford~ Mass. and a sample (0.5 ml~ was checked for sterility by bacteriologic culture. The supernate was divided into two aliquots and 7~ fetal calf serum (FSC) was added to one of these. One ml of each aliquot was heated at 56C
for 30 minutes before evaluation for cytotoxicity. The production of heat-labile toxin was determined by subtracting heat-stable toxicity from total toxicity.
When the optimum conditions for harvesting culture supernate had been determined, the stability of toxic activity was evaluated for various ccnditions of storage.

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Cytotoxicity Assay The toxic activity in culture supernate was determined by a microplate assay using as targets BL-3 cells, a bovine leukemia-derived B lymphocyte cell line obtained from G. Theilen, University of California, ~avis, California. Alternatively, freshly harvested bovine alveolar macropha~es or peripheral blood lymphocytes may be used; i.e., need ruminant leukocytes but the use of ~L-3 cells is not obli~atory. Cells were incubated in the presence of culture supernate for l hour at 37C. Cell survival at the end of the assay was assessed by stainin~ the remaining viable cells with the dye neutral red. Following solubilization of cells, dye uptake was determined as optical density (540 nm) using an automated spectrophotometer available from Titertek Multiscan, Flow Laboratories, Mississauga, Ontario. The percent toxicity for each test preparation was calculated as follows:
~ toxicity = A - B X 100 A

where A = mean OD (optical density) of quadruplicate control wells, RPMI 1640 medium only;
B = mean OD (optical density) of quadruplicate wells containing the test preparation.
By way of this assay, the relationship of leukotoxin production to the growth curve of P.
haemolytica can be evaluated in the manner illustrated in Figure l. When optimum conditions for harvesting the cytotoxin were determined, the harvested supernate was then evaluated for toxicity under various conditions of treating the isolated supernate. Untreated supernate, supernate with 7% fetal calf serum added at harvest and supernate with 7% fetal calf serum added at test were evaluated to reveal that, from the standpoint of analyzing toxicity of the supernate, the best combination is the addition of 7% fetal calf serum added at harvest to the supernate, in order to maintain 11 ~3~

toxicity of the cytotoxin for assay. A second approach of freezing the supernate upon harvesting at -70C and maintaining it at -70C also retains the activity of the cytotoxin.

Evaluation of Immunogenieity of the Cytotoxin Vaccine __ _ Having established the presence of khe cytotoxin in the culture supernate, a vaccine is prepared therefrom.
The fîltered culture supernate is lyophilized and reconstitu-ted to 5 mg/ml in sterile saline. A rapid technique to evaluate immunogenicity in animals is to conduct a study with mice wherein it is understood that with this type of cytotoxin, an immune reaction in mice confirms an immune reaction in other animals, including cattle. Balb/c mice each received by intraperitoneal injection 0,2 ml of the vaccine. The vaceine had no detectable toxic activity at the time of immunization.
An additional five control mice received a similar injection of RPMI 1640 medium, similarly lyophilized and reconstituted. Mice were given four weekly immunizations. Sera from the miee were tested for ability to neutra}ize P. ~ iea leukotoxin 5 days after the last injection. Toxin~neutralization was assessed using the neutral red assay after preincubation (1 hour, 370Cj of cytoto~ic culture supernate (toxin) prepared using 7% normal mouse serum with various dilutions of test sera. The results of the test are set out in following Table 1. The percent neutralization was calculated as:

% neutralization = C - D X 100 E - D
where C = mean OD (optieal density) of quadruplicate wells containing toxin previously ineubated with test mouse serum D = mean OD (optical density) of eontrol wells containing toxin previously incubated with pooled normal mouse serum at the same . ~2 ~3~6~
dilution as C
E = mean OD (optical density) of control wells containing RPMI 1.640 plus normal mouse serum, at the same dilution as C.
The neutral red assay involved the evaluation of uptake of neutral red dye by unkilled cells which is, therefore, a measure of the immune response in the mice.

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Toxin Neutralizing Activity in Sera from Mice Immunized With Culture Supernate from P. Haemolytica Al Grown in Serum-Free Medium_ _ % Neutralization Neutralizing Immunization MouseSerum Dilution Titer 1/~0 1/80 1/1601/320 (50~ end~oint) Culture : 1- 6-1 -L0-2--- 180 ~144 $--17320 -~
Supernate 2 98 106 114 76 ~ 1/320 3 37 106 107 121 ~1/320 4 90 97 145 99 ~1/320 Medium : 1 0 5 0 0 0 Alone 2 0 12 7 4 0 _ The leukotoxin produced in serum-free medium is immunogenic, inducing neutraIizing activity in serum even when the material used for immunization is itself not demonstrably Leukotoxic. This is a significant development insofar as the prevention of pneumonic pastereullosis. The vaccine can be prepared in a serum-free medium and, as a consequence, provide a serum-free vaccine containing leukotoxin which is inactive in its produced state yet is capable of ~; eliciting immune response when administered to animals and in particular cattle. The vaccine is usaful in the prevention of P. haemolytica pneumonia in ruminants. It is also potentially effective for treatment of other P.
haemolytica infections such as mastitis. Since stability of the leukotoxin in the vaccine is not a problem, the vaccine has excellent storage properties and because of the absence of endotoxin, does not produce severe local reactions at injection sites or anaphylactoid reactions.
Although preferred embodiments o the invention have been described herein in detail, it will be understood by - those skilled in the art that variations may be made thereto without departing from the spirit of the invention or the scope of the appended claims.

Claims (20)

1. A process for producing a non-toxic inactive cytotoxin in a serum-free medium from a culture of Pasteurella haemolytica Al and removing the cytotoxin produced therefrom, comprising:
(A) culturing Pasteurella haemolytica viable cells in a serum-free medium to produce said cytotoxin, (B) monitoring a determinant of logarithmic phase growth of said viable cells, (C) upon detecting a predetermined characteristic of said determinant which corresponds to an optimum concentration of cytotoxin produced in said serum-free medium, harvesting a liquid containing said cytotoxin from said medium; and (D) separating solids including any of said cells to provide a Pasteurella haemolytica cell-free solution of said cytotoxin.

2. A process of claim 1, wherein a serum is added to said separated liquid to stabilize said cytotoxin in said liquid for subsequent assay.

3. A process of claim 2, wherein said liquid as separated is frozen and maintained at a sufficiently low temperature to stabilize said cytotoxin for subsequent assay.

4. A process of claim 3, wherein said serum is fetal calf serum.

5. A process of claim 4, wherein said fetal calf serum is a 7% solution.

6. A process of claim 1, wherein said liquid containing cytotoxin is harvested during early to mid-logarithmic growth of said cells as indicated by detecting said predetermined characteristic.

7. The process of claim 1, wherein separation of said cytotoxin is carried out by separating a majority of solids from said liquid and filtering the remaining solids from said liquid so as to provide a bacterial cell-free solution of said cytotoxin.

8. A process of claim 7, wherein separation includes the step of centrifugation.

9. A process of claim 1, wherein said cytotoxin is a leukotoxin specific for ruminant leukocytes.

10. A process of claim 6, wherein said viable cells are P. haemolytica Al which are cultured for approximately one and one-half hours to three hours before said harvesting.

11. A process of claim 10, wherein said determinant which is monitored is optical density of said medium, measuring optical density of said medium periodically, said predetermined characteristic being a value for optical density which indicates a phase of logarithmic growth of said viable cells corresponding to said optimum concentration of said cytotoxin.

12. A process of claim 11 wherein said value for optical density is about 0.37 measured at a wavelength of 525 nm.

13. A process of claim 7, wherein said separated liquid including said non-toxic inactive cytotoxin constitutes an animal vaccine with said separated liquid being a pharmaceutically acceptable carrier for said vaccine.

14. The process of claim 13, wherein said separated liquid is lyophilized and reconstituted to at least a threefold concentration in sterile saline for injection into an animal.

15. A process of claim 14, wherein said produced cytotoxin is a leukotoxin specific for ruminant leukocytes.

16. A vaccine effective against pneumonic pasteurellosis in cattle comprising a serum-free medium containing a non-toxic inactive leukotoxin specific for ruminant leukocytes.

17. A vaccine of claim 16, wherein said medium is lyophilized or otherwise suitably concentrated for use.

18. A vaccine of claim 16, wherein said medium includes a pharmaceutically expedient adjuvant.

19. A serum-free, cell-free vaccine effective against pneumonic pasteurellosis in cattle comprising a protective amount of a non-toxic inactive cytotoxin specific for ruminant leukocytes, in combination with a serum-free pharmaceutically acceptable carrier, said cytotoxin being isolated with supernatant of a serum-free culture of Pasteurella haemolytica Serotype Al where said supernatant has cells removed therefrom and is essentially endotoxin-free.

20. A serum-free vaccine effective against pneumonic pasteurellosis in cattle containing non-toxic inactive cytotoxin when prepared by the process of any one of claims 8, 9, 10, 11, 12, 13, 14 or 15.