CA1309352C - Method and composition for reducing serum cholesterol - Google Patents

Abstract

PATENT

ABSTRACT

An orally administrable composition and method for reducing serum cholesterol levels are disclosed. A pharmaceuti-cal composition comprising a gel-forming fiber such as, for example, guar gum, psyllium seed, pectin, glucomannon, oat and barley and a mineral salt such as for example, calcium carbonate, magnesium carbonate and potassium carbonate is administered to humans to reduce serum cholesterol levels.

Description

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~309352 174/20 MET~OD AND CO.'~POSITION
FOR REDUCING SERUM CHOLESTEROL

_ P E C I F I C A T I O N_ _ _ _ _ _ _ _ _ _ BACKGROUND OF ~HE INVENTION

Field of the Invention The present invention is directed to orally administrable pharmaceutical compositions and methods for reducing serum cholesterol. More particularly, the present invention is directed to a method for reducing serum cholesterol levels in humans by the oral administration of a pharmaceutical composition containing an effective quantity of guar gum, or other gel-forming fiber, in combination with calcium carbonate or other mineral carbonates. The addition of mineral carbonates to the gel-forming fiber produces enhanced dispersion of the fiber and improves the speed of hydration in the acid environment of the stomach. The formulation according to the present invention results in significant total serum cholesterol (TC) and low-density lipoprotein (LDL) reductions when administered to humans 19 according to the recommended dosage regimen.

Objects of the Present Invention It is a principal object of the present invention to provide novel pharmaceutical compositions for oral administra-tion, which compositions are effective in reducing serum cholesterol levels. Additionally, it is a further object o~ the present invention to provide a pharmaceutical dosage formulation 27 ..hiCn contains ingredients which faci~itate more complete ~309352 dis~r~ on o~ active ingr~ie~ts in the stomach, ~acilitatirg .ore r~?id hydration of gel-orming fibers which are then r,ore bio-availa~le for reduction of serum cholesterol levels. It is a further object o~ the present invention to provide a highly dispersible, non-prescription, gel-forming fiber dietary additive which lowers serum total cholesterol (TC) levels and low density lipoprotein (LDL) levels. Lastly, it is an additional object of the present invention to provide a palatable dosage form con-taining a gel-orming fiber and mineral carbonate for the reduction of serum cholesterol levels in humans.

SUMMARY OF THE INVENTION

Generally stated, the present invention accomplishes the above-described objectives by providing a method and pharma-ceutical composition for the reduc.ion of serum cholesterol levels comprising ingestion of a composition containing an effective quantity of gel-forming fiber and a mineral carbonate to assist in the dispersion and hydration of the gel-forming fiber. The composition can be administered to hyper-cholesterolemic patients or to individuals desiring to reduce their serum total cholesterol (TC) or low density lipoproteins (LDL) irrespective of a diagnosis of hypercholesterolemia. Doses of 15 grams/day of the subject composition were administered to patients with elevated serum cholesterol lev~ls. Significant reductions in total serum cholesterol (TC) and low density 27 lipoproteins (LDL) were reported.

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_ _ _ _ _ _ _ _ In a broad aspect, the method of the present invention is based upon the surprising discovery that when a gel-for.ir.g fiber, such as for e~ample, guar gum, is combined with a mineral carbonate, such as for exa~ple, calcium carbonate, dispersion o~
the fiber in the gastrointestinal fluids is enhanced and the fiber is hydrated more rapidly and completely. As a result, the dietary fiber is more bioavailable and capable of rendering its cholesterol-reducing effects.
Despite the encouraging recent decline in coronary heart disease (CHD), it remains the number one cause of death in the United States (Kannel, W.B., Thom, T.J., "Declining Cardio-vascular Mortality", Circulation 70:331-336 (1984)). Coronary heart disease is responsible for over 550,000 deaths in the United States per year. It is responsible for more deaths than all forms of cancer combined. It has been reported that there are more than 5.4 million Americans with symptomatic coronary heart disease and a large number of bthers with undiagnosed coronary disease. Over 680,000 hospitalizations resulting from myocardial infarction occur each year in the U.S. The direct health costs associated with these figures are estimated at $8 billion with total economic costs estimated at more than $60 billion (National Institutes of Health Consensus Development Conference On r owering Blood Cholesterol To Prevent Disease, J~A: 253, No. 14, 2080 (19853) (hereinafter, NIH Consensus Conference).
The NIH Consensus Conference panel has concluded that a major cause of coronary artery disease is elevated serum cholesterol. In evaluating whether there is a causal relation-~ip bi't'~i~f~ 15~d c:~ol~sterol lrfe]s and c2ron-ry hr~t di~r2_r, t~e p~nel agreed that while it is clear that an elevated blood cholesterol level is not the only cause of coronary heart disease "[t]he evidence supporting a causal relationship between cholesterol levels and coronary heart disease comes from a wealth of congruent results o~ genetic, experimental pathologic, epidemiologic and intervention studies. These data establish beyond any reasonable doubt the close relationship between elevated blood cholesterol levels and coronary heart disease."
(~IH Consensus Conference, at page 2081.) While multiple modifiable factors associated with the development of clinical CHD have been identified, including cigarette smoking, high blood pressure, obesity, sedentary life-style, and elevated blood cholesterol, severe CHD can result from high blood cholesterol levels in the absence of any other con-tributory risk factors. (See, Dauber, T.R., The Framingham Study, Cambridge, MA, Harvard University (1980) and Thelle, D.S.
et al., "The Tromso Heart Study: Methods and Main Results of the Cross-Sectional Study." Acta. Med. Scand., 200:107-118 (1976)).
In fact, serum cholesterol concentrations may be one of the most important factors and may play a central role in the atherosclerotic process (Ross, R., Glomset, J., "The Pathogenesis of Atherosclerosis", N. Engl. J. Med., 295:369-377, 420-425 .

~1976), and Henriksen, T. Mahoney, E.M. Steinberg, D., "Interactions of Plasma Lipoproteins With Endothelial Cells", Ann. N.Y. Acad. Sci., 401:102-116 (1982)).

-The lipids in plasma of major clinical importance arecholesterol and triglycerides. Cholesterol is always present as a major ingredient in atherosclerotic plaque, along with fatty acids esters of cholesterol, phosphatides, neutral fats and di~d~cch~le~erol. Cholest~rol i. not ~iscible with wat~r.
To carr~ it in the blood, it is combined or repac~:2ged with protein. T~e cc~bination of cholesterol and protein is called a lipoprotein. Very low-density lipopcoteins (VLDL or pre-beta-lipoproteins), carry endogenously synthesized triglycerides, which are removed by muscle, heart, adipose tissue, and other sites. Major remnants of VLDL metabolism are low-density lipoproteins (LDL or beta-lipoproteins). LDLs are catabolized at cell membranes, but the major organ sites remain uncertain. It is the LDLs which contain the greatest percentage of cholesterol.
These particles, when present in excess in the blood, are deposited in the tissues and form a major part of the build-up in the arterial wall to form atherosclerotic plaque which narrows the channels of the coronary arteries which furnish the major blood supply to the heart muscle. High density lipoproteins (HDL
or alpha-lipoproteins) contain phospholipids and cholesterol complexed with apolipoproteins, the bulk of which differ from those found in VLDLs and LDLs. It is the HDLs which contain the greatest amount ~f protein and the smallest amount of cholesterol and are believed to take cholesterol away from cells and trans-port it back to the liver for processing or removal. In the postabsorptive state, a total plasma cholesterol concentration of 200 mg/dl is distributed very roughly as follows: VLDL, 10; LDL, 120; and, HDL, 50. Most of the plasma triglycerides above about sn mg/dl will be found in VLDLs.
There is no absolute definition of hyperlipidemia. For biologic variables, upper limits such as the upper five or ten percent of the distribution within the population are often used, but for plasma cholesterol, these statistical limits are too high to be used clinically. Correlations between the cholesterol 13093~;2 conc~t.~tio-5 ir ;-,l 9 r~en in ~;orth ,~meric~ 2r,d inci~nc~ o pre ature isc~ ic h~art disease indic~t~ that an increasin~ ris'~
can be detected ~hen the cholesterol is highe~ than 220 mg/dl, a val~e close to the mean for men from 40 to 49 years of age in this population. E~trapolation o~ similar data from other populations suggests that a cholesterol level at birth averages 60 mg/dl. Within one month the average has risen to about 120 mg/dl and by the first year to 175 mg/dl. A second rise begins in the third decade and continues to about age fifty in men and somewhat later in women. In other populations this cholesterol rise in adulthood is far less prominent. It is therefore not to be considered necessarily physiologic. The age-related increases in cholesterol are associated mainly with the rise in LDL concen-trations, the increases in triglycerides with a rise in VLDL.
HDL concentrations in women average about 20% higher than in men. Estrogen tends to raise and androgens tend to lower HDL
levels. The average serum cholesterol level for middle-aged adults in the U.S. is 215 mg/dl. The U.S. Health and Nutrition Examination Survey (1971-1974) indicates that the prevalence rate of a serum cholesterol level in excess of 260 mg/dl in the American male work force between the ages of 45 and 55 years is approximately 30~. (U.S. Department of Health and Human Services: Cardiovascular Primer for the Workplace, NIH
Publication No. 81-2210, 75 (January, 1981)).
Evidence tha-t actively decreasing LDL content in men with hypercholesterolemia results in a lower incidence of cardiovascular events has been greatly strengthened by the results of the Coronary Primary Prevention Trial-Lipid Research Clinic (LRC). ~his seven-year, randomized double blind trial conducted with 3,806 men convincingly demonstrated a significant re~ction o~ myoc2rdi21 infarction (~I) and sudden cardiac de2th in the group that achieved LDL reduction by means of a mGderate diet in com~ination with cholestyramine, a bile acid-sequestering resin. This investigation further demonstrated that a 2~%
reduction in total plasma cholesterol (TC~ concentration results in a 49% reduction in the frequency of myocardial infarction and sudden cardiac death. (Lipid Research Clincs Program: The Lipid Research Clinics Coronary Primary Prevention Trial Results: I.
Reduction in Incidence of Coronary Heart Disease, and II. The Relationship of Reduction in Incidence of Coronary Heart Disease to Cholesterol Lowering, JAMA, 251: 351-374 (1984)).
Accordingly, every 1% reduction in total cholesterol (TC) levels results in an approximately 2% reduction in risk of myocardial infarction.
The classic approach to cholesterol management has been to employ a two-stage treatment. In the first stage, non-pharmaceutical methods, such as low-fat diets, exercise, weight loss and alteration of environmental factors are employed. The second stage involves administration of medications which alter lipid metabolism. The hygienic methods employed in stage one are often fraught with significant problems. A common difficulty with this hygienic aspect of the classic approach is the effort required on the part of the subject to achieve a substantial reduction in plasma LDL's. Frequently, patient-initiation is resisted and long-term compliance may suffer as patients find it difficult to exercise, modify life-long dietary habits and reduce body weight. Often as a result of inadequate patient compliance with hygienic methods above, pharmacologic medications are employed in part as a result of the relative ease of use and ra~id results. ~owever, the pharmacologic methods suffer from '; i ~, . l ~ i C . . . ~ ;"~ 5 ~ . D~ }~ r~ t ~. 2'1 U~CC,.~ ,, 2.,~ can ir, sc e patients b~ c~ite se;ere. Eu~ r -e, fre~uer~tl~ 2n individual is placed on a multiple drug resi,en which ca~ result in significant drug interactions.
As a result of the significant drawbacks associated with pharm2cologic treatment of hypercholsterole~ia, an approach has been suggested which would bridge the gap between non-pharmaceutical methods and administration of pharmacologic agents to reduce cholesterol. One such suggestion has been to admin-ister cholesterol-reducing dietary additives such as gel-forming plant fibers, to individuals desiring to reduce their cholesterol levels (Superko, R.H., ~Decreasing Blood Cholesterol Levels With a Dietary Additive: An Additional Approach to Diet and Drugs,"
Cardiovascular Reviews ~ Reports, 6: No. 11,1253 (November 1985)). One such plant fiber, guar gum is obtained from the leguminous Guar plant (Cyamopsis Tetragonoloba) which grows to a height of three to six feet. The Guar plant bears bean-like pods, each of which contains six to nine small rounded seeds.
The guar seed is typically composed of 40-46~ germ, 38-45 endosperm and 14-16% husk. Guar gum is produced from the endosperm of the guar seed. Chemically, guar gum is a galacto-mannon storage polysaccharide composed of mannose and galactose groups. It is commercially available from, for example, Henkel Corporation as Supercol~ Guar Gum in a variety of powder formulations with different granulations and terminal viscosi-ties. Guar gum is used extensively in the food industry and is recognized as safe by the Food and Drug Administration. Further-more, guar gum has been shown to reduce serum cholesterol levels in normal and hypercholesterolemic subjects. However, problems such as poor dispersibility have been associated with oral dosage for~ ch 25 t~let5 or c'?s~ 7~) GC p~lr~ g~r 5~.~ or with c~h~r ~ bers such as psylliu.~ seed, pectin or oat.
Further..o-e, p.oble.~,s 2ssociated with its highly viscous nature have been r~c-gnized and have hampered the long-term clinical application of guar gum as a palatable cholesterol-reducing agent (Khan, A.R. et al., ~Effect of Guar Gum on 2100d Lipids," The ~erican Journal of Clinical Nutrition, 34:2446-2449 (November, 1981)). Laboratory experiments on capsule formulations of pure guar gum in gastric simulators which approximate the conditions present in the stomach, indicate that as the capsule dissolves, and hydration of the gum begins, only the powder around the perimeter of the dosage hydrates or gels leaving an unhydrated inner core of gum. This incomplete dispersion leaves perhaps as much as 30~ to 40% of the guar fiber unhydrated.
The mechanism of action of guar gum as a plasma cholesterol-reducing agent is unclear. However, the mode of action appears to be associated wlth its viscosity. It has been postulated that the viscous guar gum may coat the intestinal mucosa and interfere with adsorption of lipids which may be related to an increase in resistance of the unstirred water layer and limit the intestinal bulk phase diffusion. (See, Modaky, S., "Effect of Dietary Pectin and Algin on Blood Cholesterol Level in Growing Rats Fed on Cholesterol-Free Diet," Nutr. Metabol., 15:290-294 (1973), Kay, R.M. et al., "Effect of Citrus Pectin on Blood Lipids and Feca-l Steroid Excretion in Man," Am. J. Clin.
Nutr., 30:111-175 (1977).) One study found that the presence of a polysaccharide gum in the fluid film surrounding the villi in rat jejunum increases its viscosity and gives rise to a thicken-ing of the rate-limiting unstirred layer in vitro (Johnson et al., "Effect of Gel-Forming Gums on the Intestinal Unstirred 1309~352 L~r ~ 5~ n~s t in Vitro,~ G~t, 22.3~8-;~3 (~
O~h~r ~or~ Gn r2ts re~.~ealed an elevation of enzy~e acti~Jity in the int~stire that ~ay be due to a slower rate o~ enzy~e deqrada-tion. Hu~an evidence also suggests an in vitro inhibition of pancreatic enzyme activity by dietary fibers. Trypsin, amylase, lipase and phospholipidase activities were reduced in human duodenal juice following exposure to various fibers including guar (Isaksson, G. et al., "In Vitro Inhibition of Pancreatic Enzyme Activities by Dietary Fiber," Diqestion, 24:54-59 (1982)).
Additional possibilities for the gel forming fiber's mechanism of action may include bile acid sequestration, fat adsorption sites, facilitation of gallbladder emptying and gastrointestinal motility. There has also been some speculation that hypercholesterolemic activity may be related to diminished caloric intake following administration of these types of fibers. Vpon hydration, the fibers gel and provide a full feeling in the stomach which may contribute to a reduction in dietary intake and weight loss. These effects may contribute to reduced blood sugar and cholesterol Ievels. However, work on rats has suggested that bile salt 105s associated with steator-rhea may be the mechanism by which pectin lowers the serum cholesterol. Certain studies indicate that both guar gum and pectin administration was also associated with greatly increased fat loss in the stool. (Jenkins, D.J. et al., "Effect of Pectin, Guar Gum, and ~heat Fibre on Serum-Cholesterol", The Lancet, 1116-1117, (May 17, 1975)).
In Superko, supra, at page 1257, a compilation of clinical studies on the effects of guar gum on blood lipids in humans was collected. The respective studies indicate that a series of different formulations of guar gum such as granules, crispbread and capsules, were administered in various dosages over treatment durations ranging from one week to four months. The results indicated that total serum cholesterol reduction ranged from a nonstatistically significant 3.4~ up to as high as 16.6~.
Table I illustrates the significantly greater choles-terol-reducing properties of one of the dosage forms according to the present invention. 15 grams of guar gum and calcium carbonate in a capsule formulation in a 5:1 ratio was administered in three divided doses of 5 grams at mealtime to patients with elevated serum cholesterol levels.
TABLE I

Total Percent Low Density (LDL) Percent Patient cholesterol (TC) Reduction (TC~ Li~o~rotein (LDL~ Reduction A.

Baseline 289mg/dl 165mg/dl After 2 wks 204mg/dl29% 86mg/dl 48%

After 4 wks 209mg/dl28% 98mg/dl 41%

8.

Baseline 283r.lg/dl 208mg/dl After 2 wks 220mg/dl22~ 157mg/dl 25 After 4 wks 206mg/dl27% 130mg/dl 38%

Taking into consideration the results of the Lipid Research Clinic (LRC) Program, supra, which demonstrated a 49% reduction in the frequency of myocardial infarction and sudden cardiac death with every 25~ reduction in total plasma cholesterol, the significant reductions in choles-terol reported in Table I are encouraging.
It is understood that compositions according to the present invention may contain optional preservatives, sweeteners or flavorants which may provide a more palatable dosage form and assist in long-term patient compliance.

th^~ry, it i; ~ la~d ttl~t t~e ~or~lati-,n according to the present inJen-ion is more rapidly dispersed and t~ereby more avail~ble for its cholesterol-reducing effects. It is postulated that the formulat-on according to the method of the present invention eYhibits a physical/chemical interaction whereby the mineral carbonate ions interact with the gel which is formed when the guar gum hydrates. Normally, with pure guar gum fiber, bile acid anions diffuse through the gel. With the formulation according to the present invention, the mineral ions, calcium, magnesium and potassium, precipitate the bile acids entrapping, entangling and enmeshing them. The liver then produces more bile acids. Since the liver uses cholesterol to make bile acids, the cholesterol pool becomes depleted, thereby lowering cholesterol levels. Furthermore, ~he action of the mineral carbonate causes release oE carbon dioxide in the stomach which facilitates the rapid dispersion of the guar formulation thereby causing better, more complete and more rapid hydration of the guar fiber. Addi-tionally, the speed of hydration of the guar fiber is enhanced as a result of the more rapid dispersion. The prior art problems of incomplete hydration with an unhydrated inner core are avoided by the composition according to the present invention.
The present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof and, accordingly, reference should be made to the appended claims, rather than to the foregoing specification, as 26 indicating the scope of the invention.

Claims (12)

1. An orally administrable pharmaceutical composition for use in reducing serum cholesterol levels consisting essentially of:
(a) an effective amount of a gel-forming fiber which exhibits cholesterol reducing activity, and (b) an orally-ingestible non-toxic mineral salt capable of dissolution in the gastric fluid with release of carbon dioxide.

2. A composition according to claim 1 comprising a flavorant, sweetener, or preservative.

3. A composition according to claim 1 wherein said gel-forming fiber is a member selected from the group consisting of guar gum, psyllium seed, pectin, glucomannon, oat and barley.

4. A composition according to claim 1 wherein said mineral salt is a member selected from the group consisting of calcium carbonate, magnesium carbonate, and potassium carbonate.

5. A composition according to claim 1 wherein the ratio of said gel-forming fiber to said mineral salt is in the range of approximately 3:1 to approximately 10:1.

6. An orally administrable pharmaceutical composition for use in reducing serum cholesterol levels consisting essentially of an effective amount of a gel-forming fiber - 13 - HK 1 Ca/bam selected from the group consisting of guar gum, psyllium seed, pectin, glucomannon, oat and barley, and an orally-ingestible non-toxic mineral salt capable of dissolution in the gastrointestinal fluid with release of carbon dioxide.

7. A method for the preparation of a pharmaceutical composition useful for lowering serum cholesterol in humans consisting essentially of admixing into an oral dosage unit an effective amount of gel-forming fiber and an orally-ingestible non-toxic mineral salt capable of dissolution in human gastric fluid with release of carbon dioxide.

8. The method according to claim 7 wherein said gel-forming fiber is a member selected from the group consist-ing of guar gum, psyllium seed, pectin, glucomannon, oat and barley.

9. The method according to claim 7 wherein said salt is a mineral carbonate selected from the group consisting of calcium carbonate, magnesium carbonate, and potassium carbonate.

10. The method according to claim 7 wherein the ratio of said gel-forming fiber to said mineral salt is in the range of approximately 3:1 to approximately 10:1.

11. The method according to claim 7 wherein said oral dosage unit also comprises a flavorant, sweetener, or preservative.

12. A method for the preparation of a pharmaceutical composition useful for lowering serum cholesterol consist-ing essentially of admixing into an oral dosage unit, an effective amount of a gel-forming fiber selected from the group consisting of guar gum, psyllium seed, pectin, glucomannon, oat and barley and an orally-ingestible, non-toxic mineral carbonate capable of dissolution in human gastric fluid with release of carbon dioxide.

- 14 - HK 1 Ca/bam