CA1308411C - Pyrido ¬2,3-d|pyrimidines derivatives - Google Patents

Abstract

ABSTRACT OF THE DISCLOSURE

A compound of the formula:

wherein R1 is amino or hydroxy;
R3 is hydrogen, methyl, or ethyl; and Z is hydroxy, alkoxy, or -NH-C*(COOX)-CH2CH2COOX in which X is hydrogen or alkyl and the configuration about the carbon atom designated *
is L. These compounds are useful as intermediates in making pyrido[2,3-d]pyrimidines of the formula:

IA
wherein R1 is amino or hydroxy; and R3 is hydrogen, methyl, or ethyl;
the configuration about the carbon atom designated being L;
(ib) 5,6,7,8-tetrahydropyrido[2,3-d]-pyrimidines of the formula:

IB

wherein R1 is amino or hydroxy; and R3 is hydrogen, methyl, or ethyl;
the configuration about the carbon atom designated *
being L;
(ii) the tautomeric forms thereof; and (iii) the pharmaceutically acceptable alkali metal, alkaline earth metal, non-toxic metal, ammonium, and substituted ammonium salts thereof are disclosed.

Description

1 3~,~4 1 1 This application is a divisional application of Canadian Patent Application 503,509, filed March 8, 1985.
Description PYRIDO r 2.3-dlPYRIMIDINE DERI~ATIVES
Technical Field The invention pertains to derivatives of N-(4-[l-(pyrido[2,3-d]pyrimidin-6-yl)alk-2-yl]benzoyl)-L-glutamic acid, which derivatives are antineoplastic agents, and to their preparation and use.
Background Art The folic acid antimetabolites aminopterin and amethopterin (also known as 10-methylaminopterin or methotrexate) are antineoplastic agents. These compounds inhibit enzymatic conversions involving metabolic derivatives of folic acid. Amethopterin, for example, inhibits dihydrofolate reductase, an enzyme necessary for the regeneration of te~rahydrofolate -from the dihydro-folate which is formed during the conversion of 2-de-oxyuridylate to thymidylate by the enzyme thymidylate synthetase.
Other derivatives of folic acid and aminopterin have been synthesized and tested as antimetabolites.
Among these are various "deaza" compounds in which a methylene or methylidene group occupies a position in the molecule normally occupied by an imino or nitrilo group, respectively. These derivatives have varying degrees of antimetabolic activity. 10-Deaza-aminopterin i5 highly active (Sirotak et al., Cancer Treat. Rep., 1978, 62, 1047) whereas 10-deazafolic acid shows no significant activity (Struck et al., J. Med. Chem. 1971, 14, 693).
5-Deazafolic acid is only weakly cytotoxic whereas 5-deazaaminopterin has activity similar to that of amethopterin (Taylor et al, J. Orq. Chem., 1983, 48, 4852). 8,10-Dideazafolic acid is only marginally effective as a dihydrofolate reductase inhibitor (De Graw et al., "Chemistry and Biology of `-~Jk 1 "~'3~1 1 Pteridines", Elsevier, 1979, 229) while 5,8,10-trideaza-folic acid also shows only marginal activity against mouse L1210 leukemia tOatis et al., J. Med. Chem., 1977, 20, 1393). 8,10-Dideazaaminopterin is reported to be active (U.S. Patent ~o. 4,460,591) and 5,8,10-trideaza-aminopterin exhibits activity against mouse L1210 leukemia (Yan et al., J. Heterocycl Chem., 1979, 16, 541).

Disclosure of Invention The invention pertains to ~ia) pyrido[2,3-d]pyrimidines of the formula:

~ R3~3-co~H-cH-cH2cH2cooH

IA
wherein Rl is amino or hydroxy; and R3 is hydrogen, methyl, or ethyl;
the configuration about the carbon atom designated *
being I.;
(ib) 5,6,7,8-tetrahydropyrido[2,3-d]-pyrimidines of the formula:
Rl R3 ~CH2-cH~3~_coNH-cH-cH CH2COOH

H

IB

1 "",41 1 wherein Rl is amino or hydroxy; and R3 is hydrogen, methyl, or ethyl;
the configuration about the carbon atom designated *
being L;
(ii) the tautomeric forms thereof; and (iii) the pharmaceutically acceptable alkali metal, alkaline earth metal, non-toxic metal, ammonium, and substituted ammonium salts thereof.
The invention also pertains to methods for the preparation of such compounds, to intermediates useful in those preparations, and to methods and compositions for the use of such compounds in combatting neoplastic growth.
The intermediates useful in the preparation of such compounds are compounds of the formula:

H N ~ ~ CH~ ~ -COZ
wherein R1 is amino or hydroxy;
R3 is hydrogen, methyl, or ethyl; and Z is hydroxy, alkoxy, or -NH-C*(COOX)--CH2CH2COOX in which X is hydrogen or alkyl and the configuration about the carbon atom designated *
is L.
Modes For Carryina Out The Invention The compounds of the invention are derivatives of the pyrido[2,3-d]pyrimidine heterocyclic ring which is numbered as follows:

1 ~IJ~1 1 -3a-2 1 ~ 7 The compounds of Formulas IA and IB in which Rl is hydroxy exist in tautomeric equilibrium with the corresponding 3,4-dihydro-4~oxo compounds.
OH O
N ~ 2 i5 For convenience, the 4-hydroxy form is depicted and the corresponding nomenclature is used throughout this specification, it being understood that 1 ~ ~3~ 1 1 ~, in each case such includes the tautomeric 3,4-dihydro-4-keto form.
The absolute configuration about the carbon atom designated * in the glutamic acid chain is L, being S the same absolute configuration as that about the cor-responding alpha carbon atom in alanine.
When R3 is other than hydrogen, a second chiral center is present, thereby producing d,L- and l,L-diastereoisomers. These can be separated mechan-ically, as by chromatography. In the case of the5,6,7,8-tetrahydro compounds of Formula IB, the carbon atom in the 6-position of the pyridol2,3-d]pyrimidine ring system also is a chiral center, leading to d,L- and l,L-diastereoisomers if R3 is hydroqen and to d,l,L-, d,d,L-, l,l,L-, and l,d,L-diastereoisomers if R3 is other than hydrogen. All of the above forms, which can be separated as described above, are within the scope of the invention.
The invention includes the pharmaceutically acceptable alkali metal, alkaline earth metal, non-toxic metal, ammonium, and substituted ammonium salts, such as for example the sodium, potassium, lithium, calcium, magnesium, aluminum, zinc, ammonium, trimethylammonium, triethylammonium, triethanolammonium, pyridinium, sub stituted pyridinium, and the like.
The compounds of this invention have an effect on one or more enzymes which utilize folic acid, and in particular metabolic derivatives of folic acid, as a substrate. The following compounds are representative:
30 Compound No. 1. N (4-[2-(2,4-diaminopyrido[203-d]-pyrimidin-6-yl)ethyl]benzoyl)-L-glutamic acid.

Compound No. 2. N-~4-[2-(2-amino-4-hydroxypyrido-12~3-d~pyrimidin-6-yl)ethyl]benzoyl)-L-glutamic acid.

1 3n?)4 1 1 Compound No. 3. N-t4~12~(2~4~diaminO~5~6~7~8~tetra~
hydropyridol2,3-d]pyrimidin-6-yl)-ethyl]benzoyl)-L~glutamic acid.

Compound No. 4. N-(4-l2-(2-amino-4-hydroxy-5,6,7,8-tetrahydropyrido[2,3-d]pyrimidin-6-yl)-ethyl]benzoyl~-L-glutamic acid.

Table 1 Inhibition of Dihydrofolate Reductase (DHFR) (Kaufman et al., "Methods in Enzymology~, 10Jacobs and Wilcheck, Eds., Academic Press:
New York, 1974, pp 272-281) Compound IC50M

4.3xlO 8 2 4.9xlO 5 3 7.1xlO 8 4 5~6xlO

Table 2 Inhibition of Thymidylate Synthetase 20(Wahba et al., J. Biol. Chem., 1961, 236, p 611) Compound IC50M

9.2xlO 5 2 7.7xlO 5 3 9.2xlO 4 4 >lxlO 3 1 ') '` ~ 4 1 1 Table 3 Substrate for Folate Polyglutamate Synthetase Incubation with partially purified mouse liver FPGS Ispecific activity = 1.2 nmol h 1 (mg of protein)]
for one hour at 37C; full saturation curves obtained for duplicate assay~ at 6 concentrations - see Moran et al., Anal. Biochem., 1984, 146, 326.

Compound *

10 1 0.86 + 0.11 0.35 + 0.02 2 0.68 + 0.14 0.90 + ~.10 3 0.23 + 0.01 1.61 + 0.05 4 0.05 + 0.02 1.24 - 0.10 FH4 0.05 - O.Olt 1.31 - 0.07t * Relative to folic acid.
t Published data, see Moran et al., Biochemistry, 1984, 23, 4580.

Table 4 Inhibition of L1210 murine leukemic cells in tissue culture - see Foley et al., Biochem. Pharmacol., 1967, 16, 65~.
Compound IC50M

1 1.7xlO 8 25 2 >10 4 3 3.3xlO 9 5.9xlO 8 Table 5 Increase in life span (ILS) in mice (BDFl) following peritoneal injection of 10 L1210 leukemia cells. Compounds administered intraperitoneally for 9 days at indicated dosage.

1 7"341 1 CompoundDose (mg/kg) ~ ILS

N- ~4-l2-~2-amino-4-hydroxy-5,6,7,8-tetrahydro-pyridol2,3-d]pyrimidin-6-yl)-ethyl~benzoyl)-L-glutamic acid in particular is a unique antimetabolite. While maintaining good activity against L-1210 leukemia which is comparable to methotrexate, the compound is a weak inhibitor of dihydrofolate reductase, indicating prob-able activity against the folate-related en~yme targets other than DHFR. This conclusion is supported by its activity against methotrexate-resistant cells.

Table 6 Effect on Methotrexate-Resistant L-1210 Leukemia Mean Dose Increased Compound (mg/kg)Life span in d~s control - 0 Methotrexate 2 0 4 2 +8 4 4 ~11 4 ~ ~17 4 12 ~25 The compounds can be prepared by hydrolysis or hydrogenolysis of a glutamic acid derivative of the formula:

1 ~0'34 ~ ~

R4-CH2C3~3-CoNH-CHCH2CH2CooR6 II
in which R and R3 are as previously defined;
R lS R

N ~

R HNN N

R HNN N
H
R5 and R6 are the same or different carboxylic acid protecting group; and R is hydrogen or an amino acid protecting group.

The hydrolysis is conducted at normal temp-eratures utilizing aqueous acid or base, such as for example, an aqueous alkali metal hydroxide, optionally in the presence of a water miscible organic solvent such as methanol, ethanol, tetrahydrofuran, dimethyl-formamide, and the like, or an acid, as for example trifluoroacetic acid. When base is used, the product is initially formed as the dicationic glutamate salt and can be readily precipitated by adjustment of pH, as 1, 3 o ~ 1 1 through acidification with, for example, acetic acid.
The resulting products generally are high melting crystalline or microcrystalline solids.
The glutamic acid intermediate of Formula II
can be obtained by hydrogenating a pyrido[2,3-d]-pyrimidine compound of the formula:

N ~ CH=C ~ CONH--CH-CH2CH2COOR6 III

wherein Rl, R3, R5, R6 and R are as previously de-fined. The hydrogenation can be performed at from 50 to 100 psi in an inert solvent and in the presence of a suitable catalyst such as the noble metals or metal oxides such as palladium or platinum oxide, optionally on a support such as carbon or calcium carbonate; e.g.
Pd/C, Pd/CaCO3, PtO2.
The 5,6,7,8-tetrahydropyridol2,3-d~pyrimidinyl intermediate of Formula II in which R is ~ ~

can be produced by independent hydrogenation of the corresponding compound of Formula II wherein R is 1 3~4 1 1 R

R HN l ~

Alternatively, a 5,6,7,8-tetrahydropyrido-12,3-d]pyrimidinyl intermediate of Formula II can be formed directly in the hydrogenation of the pyrido-12,3-d]pyrimidine intermediate of Formula III through the use of more vigorous conditions, such as increasing ~he hydrogenation time, increasing the pressure and/or raising the temperature.
The intermediate of Formula III can be pre-pared in several ways. In one embodiment, a benzoic acid derivative Rl R3 ~ CH=C ~ COOH

R HN N N
IV
is coupled with a protected glutamic acid derivative of the formula utilizing conventional condensation techniques for forming peptide bonds, such as activation of the carboxylic acid through formation of the mixed anhydride, treatment with DCC, or use of diphenylchloro-phosphonate.

1 ~ J3~ 1 1 Alternatively an aldehyde of the formula Rl R HN ~ ~ CHO

VI
(see Taylor et al., J. Org. Chem, 1983, 48, 4852) can be coupled with a Wittig reagent of the formula (C6 5)3 ~ CONHCHCH2CH2COOR
COOR

VII

(see Yan, J. Het. Chem., 1979, 16, 541) in the presence of sodium hydride, or another strong non-nucleophilic base, in a solvent such as N-methy1pyrrolidone or dimethylformamide. The reverse reaction also can be employed, namely the reaction of a Wittig reagent of the formula R
1 ~
N ~ ~CH2P(C6H5)3 R7HN ~ N~ N ~

VIII
with a N-(4-formyl- or 4-alkanoylbenzoy1)-L-glutamic acid in which the carboxylic acid groups are protected.

1 303~ 1 1 The benzoic acid derivative of Formula IV can be prepared by cyclization of a 4-~1-(2-substituted 3-cyano-pyridin-5-yl)alk-1-en-2-yl]benzoate of the formula N C ~ CH=C ~ -COO-alkyl IX
in which R8 is amino or 4-nitrophenylthio, with guanidine in t-butanol and an equimolar amount of an alkali metal t-butoxide such as sodium or potassium t-butoxide. Generally, the benzoate ester is a t-butyl ester. Other alkoxide-alcohol combinations can also be used for the guanidine cyclization reaction, but care should be taken to minimize transesterification. The product of this cyclization is a 4-[1-(2,4-diamino-pyrido[2,3-d]pyrimidin-6-yl)alk-en-2-yl]benzoate of the formula H2NJ~CH=C~-COO-alkyl The benzoate of Formula X can be hydrolyzed with acid such as aqueous formic acid to yield the corresponding benzoic acid derivative of Formula IV in which Rl is amino and R7 is hydrogen. The 2,4-diamino compounds of Formula IV are converted to the corres-ponding 2-amino-4-hydroxy compound through treatment with base. It is desirable first to protect the 2-amino ~3~

group through conversion to the acetamido group. Hence treatment with acetic anhydride in the presence of a hydrogen acceptor such as 4-dimethylaminopyridine results in acylation of the 2-amino group and formation of a benzoic acid mixed anhydride, the latter being hydrolyYed with base to regenerate the free benzoic acid derivative of Formula IV. Treatment with base such as 1 N sodium hydroxide then generates the corresponding 4-hydroxy compound.
Intermediate IX also can be prepared through use of a Wittig reagent. Thus [2-(4-nitrophenylthio)-3-cyanopyridin-5-ylmethyl]triphenylmethylphosphonium bromide can be obtained according to Taylor et al., J.
Org. Chem., 1983, 48, 4852 by condensation of 2-methyl-3-ethoxyacrolein and alpha-cyanothioacetamide to yield 3-cyano-S-methy].-2(lH)pyridinethione, treatment of the product with 4-nitrofluorobenzene to yield 2-(4-nitro-phenylthio)-3-cyano-5-methylpyridine, bromination with N-bromosuccinimide to yield 2-(4-nitrophenylthio)-3-cyano-5-bromomethylpyridine, and addition of triphenyl-phosphine. This compound is then coupled with a compound of the formula O=C ~ -COO-alkyl XI
in which R is as previously defined.
Amino and carboxylic acid protecting groups are described for example by Greene in "Protective Groups in Organic Synthesis", John Wiley & Sons, Inc., 1981, and McOmie in "Protective Groups in Organic Chemistry", Plenum Press, 1983.
The compounds of Formula IA and IB can be used, alone or in combination, to treat neoplasms which in the past have been treated with methotrexate, inclu-ding choriocarcinoma, leukemia, adenocarcinoma of the 1 ' "34 1 1 female breast, epidermid cancers of the head and neck, squamous or small-cell lung cancer, and various lympho-sarcomas. The compounds can also be used to treat mycosis fungoides and psoriasis which are responsive to methotrexate.
The compounds may be administered either orally or preferably parenterally, alone or in combina-tion with other anti-neoplastic agents, steroids, etc., to a mammal suffering from neoplasm and in need of treatment. Parenteral routes of administration include intramuscular, intrathecal, intravenous or intra-arterial. In general, the drug is administered in much the same fashion as methotrexate, but because of its different mode of action N-(4-[2-(2-amino-4-hydroxy-5,6,7,8-tetrahydropyrido[2,3-d]pyrimidin-6-yl)ethyl~-benzoyl)-L-glutamic acid can be administered in higher dosages than those usually employed with methotrexate Leucovorin rescue is not needed. Dosage regimens must be titrated to the particular neoplasm, the condition of the patient, and the response but generally doses will be from about 10 to about 100 mg/day for 5-10 days or single daily administration of 250-500 mg, repeated periodically; e.g. every 14 days. Oral dosage forms include tablets and capsules containing from 1-10 mg of drug per unit dosage. Isotonic saline solutions con-taining 20-100 mg/ml can be used for parenteral admini-stration.
The following examples will serve to further illustrate the invention. In the NMR data, "s" denotes singlet, "d" denotes doublet, "t" denotes triplet, "q"
denotes quartet, "m" denotes multiplet, and ~br" denotes a broad peak.

1 3''3~1 1 Example 1 13-Cyano-2-(4-nitrophenylthio)-S-pyridinyl-methyl]triphenylphosphonium Br~mide:

A. A mixture of 60.00 9 (0.221 mol) of 3-cyano-2-(4-nitrophenylthio)-5-methylpyridine, 39.37 9 (0.221 mol) of N-bromosuccinimide, 3.0 9 of benzoyl peroxide and 60 mL of benzene was refluxed for 16 hours while being irradiated with a 275-W sunlamp. The sol-vent was removed under reduced pressure and the residue was shaken with a mixture of 1 L of water and 1 L of methylene chloride. The organic layer was separated, washed with 1 L of water, dried over anhydrous magnesium sulfate, and filtered. ~emoval of the solvent by evap-oration under reduced pressure yielded 3-cyano-2-(4-nitrophenylthio)-S-bromomethylpyridine which can be used in the following step without further purification.

B. The solid obtained in Part A was stirred at room temperature with a ~olution of 58.01 9 (0.221 mol) of triphenylphosphine in 500 mL of benzene. Fil-20 tration of the reaction mixture gave 77.63 9 of [3-cyano-2-(4-nitrophenylthio)-5-pyridinylmethyl]tri-phenylphosphonium bromide.

Stirring of the mother liquor at room tempera-ture for 6 hours afforded an additional 5.67 9, (total 2S yield 83.30 9, 62%). Recrystallization from acetonitrile gave 13-cyano-2-(4-nitrophenylthio)-5-pyridinylmethyl]-triphenylphosphonium bromide as light yellow crystals, mp ~ 200C, with resolidification, mp 253-256C with dec.

C. Alternatively, 3-cyano-2-(4-nitrophenyl-thio)-5-bromomethylpyridine is allowed to react in tetrahydrofuran with tri-(n-butyl)phosphine for ten 1 30~

hours. Following the addition of ether, the solid which forms is collected by filtration and washed with 1:1 tetrahydrofuran:ether to yield ~3-cyano-2-~4-nitro-phenylthio)pyridin-5-ylmethyl]-tri-(n-butyl)phosphonium bromide as a white solid; mp 175-176C; NMR (CDC13, 80 MHz) d 0.8S-2.63(m, 27H), 4.76(d, 2H, J=15.4 Hz), 7.74(d, 2H, J=9.0 Hz), 8.26(d, 2H, J=9.0 Hz), ~.55 (brs, lH), 8.79 (brs, lH); IR (KBr) 2950, 2860, 2220, 1595, 1575, 1515, 1390, 1340, 1075 and 845 cm 1; HRMS
471 2116(M+-HBr), Calc'd. for C25H34 3 2 Example 2 3-Cyano-2-(4-nitrophenylthio)-5-[2-(4-ethoxy-carbonylphenyl)ethenyl]pyridine.

A. A mixture of 4.544 9 (7.42 mmol) of [3-cyano-2-(4-nitrophenylthio)-5-pyridinylmethyl]tri-phenylphosphonium bromide, 0.751 9 (7.42 mmol) of tri-ethylamine and 50 mL of chloroform was stirred at room temperature for 15 minutes and 1.322 g (7.42 mmol) of 4-ethoxycarbonylbenzaldehyde then were added. After stirring at room temperature for 96 hours, 100 mL of water were added, the mixture was filtered, and the organic layer was separated and washed twice with 100 mL
portions of water, dried and filtered. ~vaporation of the filtrate under reduced pressure gave a residue which was chromatographed on silica gel. Unreacted aldehyde was eluted with 2:1 petroleum ether:benzene, while the title compound, alternatively named as 2-(4-nitrophenyl-thio)-3-cyano-5-(4-ethoxycarbonylstyryl)pyridine, was eluted with benzene. Evaporation of the benzene eluate gave 2.82 g (88%) of 3-cyano-2-(4-nitrophenylthio)-5-l2-(4-ethoxycarbonylphenyl)ethenyl]pyridine as a light yellow solid. The product turns from a solid to a gum ; below 100C and then to a clear liquid between 180 and 220C; NMR (Me2SO-d6) delta 1.34 ~t, 3H, J=6.3 Hz), 4.32 1 7`~$~

tq, 2H, J-6.3 Hz), 6.73 (d, lH, J=13 Hz), 6.99 (d, lH, J=13 Hz), 7.27 (d, 2H, J=9Hz), 7.74, 7.85, 7.94 (dd, 2H, 2H), 8.26, 8.31 (dd, 2H, lH), 8.38 Id, lH, J=1.8 Hz); IR
(RBr) 2220, 1707, 1605, 1597, 1575, 1512, 1344, 1295-1277, 1174 cm 1.

Anal.: Calc'd. for C23H17N3O4S: C, 64.08; H, 3.97; N, 9.74; S, 7.43. Found: C, 63.82; H, 4.01; N, 9.51; S, 7.38.

B. 3-Cyano-2-(4-nitrophenylthio)-5-12-(4-tert-butoxycarbonylphenyl)ethenyl]pyridine was prepared in 81% yield by the above method utilizing however 4-(tert-butoxycarbonyl)benzaldehyde in place of 4-ethoxy-carbonylbenzaldehyde; mp indefinite (cis-trans mixture);
NMR (CDC13) delta 1.62 (s, 9H), 6.43 (d, lH, J=13 Hz), lS 6.90 (d, lH, J-13 Hz), 7.24 (d, 2H, J=9 Hz), 7.69 (d, 2H, J=8.1 Hz), 7.76 (d, lH, J=2.7 Hz), 7.92 (d, 2H, J-8.1 Hz), 8.22 (d, 2H, J=9 Hz), 8.34 (d, lH, J=2.7 Hz);
IR (KBr) 2220, 1707, 1600, 1577, 1518, 1341, 1290, 1163 cm Anal.: Calc'd. for C25H21N3O4S: C, 65.35; H, 4.61; N, 9.14; S, 6.98. Found: C, 65.28; H, 4.68; N, 9.20: S, 6.93.

In a similar fashion by substituting a 4-alkoxycarbonylacetophenone or a 4-alkoxycarbonyl-propiophenone for 4-ethoxycarbonylbenzaldehyde, there is respectively obtained the correspondin~ 3-cyano-2-(4-nitrophenylthio)-5-[2-(4-alkoxycarbonylphenyl)prop-1-enyl]pyridine and 3-cyano-2-(4-nitrophenylthio)-5-[2-(4-alkoxycarbonylphenyl)but-1-enyl]pyridine com-pounds. This can be exemplified as follows:

To a solution of 18.89 9 of [3-cyano-2-(4-nitrophenylthio)-5-pyridinylmethyl]-tri-(n-butyl)phos-l Q,411 phonium bromide in 150 mL of dry methylene chlôride were added in several small portions 5.36 mL of 1,5-diaza-bicyclol5.4.0]undec-5-ene. After stirring the reaction mixture under a nitrogen atmosphere for lS minutes, 7.53 9 of 4-(t-butoxycarbonyl)acetophenone ~alternatively named as tert-butyl 4-acetylbenzoate) were added. The mixture was heated under re~lux for 72 hours, cooled to room temperature, and extracted with a saturated sodium chloride solution. The extracts were dried over anhydrous sodium sulfate and then chromatographed on flash silica gel using methylene chloride as the eluent.
The eluate was concentrated under reduced pressure and the residue was triturated with ether. The resulting solid was collected by filtration to yield 5.64 9 (35%) 15 of trans-3-cyano-2-[t4-nitrophenylthio)]-5-[2-(4-tert-butoxycarbonylphenyl)prop-l-enyl]pyridine as a pale yellowish solid: mp 180-181.5C (benzene-ether); NMR
(CDC13, 250 MHz) d 1.61 ~s, 9H), 2.29 (d, 3H, J=1.23 Hz), 6.70 (brs, lH), 7.52 (d, lH, J=8.62 Hz), 7.74 (d, 20 lH, J=8.84 Hz), 7.92 (d, lH, J=2.09 Hz), 8.00 (d, lH, J=8.62 Hz), 8.27 (d, lH, J=8.84 Hz), 8.53 (d, lH, J=2.09 Hz); IR (KBr) 3060, 2970, 2220, 1695, 1595, 1515, 1425, 1380, 1365, 1340, 1290, 1160, 1110, 1010 and 840 cm 1.

Anal. Calc'd. for C26H23N3O4S: C, 65.94; H, 25 4.90; N, 8.87; S, 6.77. Found: C, 66.88; H, 4.64; N, 8.51; S, 6.77.

Concentration of the filtrate under reduced pressure and trituration of the residue, followed by filtration and washing with ether, yielded 2.08 g (13~) 30 of the cis-3-cyano-2-(4-nitrophenylthio)-5-l2-(4-tert-butoxycarbonylphenyl)prop-l-enyl]pyridine as a pale yellowish solid, mp 126~127C (ethyl acetate-hexane);
NMR (CDC13, 250 MHz) d 1.60 (s, 9H), 2.25 (d, 3H, J=1.41 Hz), 6.39 (brs), 7.18 (d, lH, J=8.28 Hz), 7.41 (d, lH, 35 J=2.22 Hz), 7.63 (d, lH, J=8.91 Hz), 7.95 (d, lH, J=8.28 .

Hz), 8.09 ld, lH, J=2.22 Hz), 8.23 td, lH, J=8.91 Hz);
IR (KBr) 3110, 2970, 2225, 1720, 1520, 1345, 1165, 1105, 920 and 840 cm 1.

Anal. Calc~d. for C26H23N3O4S: C, 65.94; H, 4.90~ N, 8.87; S, 6.77. Found: C, 65.92; H, 4.81; N, 8.62; S, 656.

The 4-(t-butoxycarbonyl)acetophenone utilized in the foregoing procedure can be prepared as follows:

To a suspension of 1.64 9 of 4-acetylbenzoic acid in 30 mL of dry benzene were added 3.0 mL of freshly distilled thionyl chloride. The mixture was heated under reflux for 5 hours. The reaction mixture was cooled to room temperature and the solvent was re-moved under reduced pressure. The resulting residue was dissolved in 5 mL of dry methylene chloride and the solution was added to a mixture of 1.11 9 of dry tert-butanol and 1.42 9 of dry pyridine. After stirring the reaction ~ixture under a nitrogen atmosphere for 15 hours, the mixture was diluted with methylene chloride and ex~racted with water. The organic solution was dried over anhydrous sodium sulfate and the solvent was removed under reduced pressure. The residue was chro-matographed on a column of silica gel using a 20% ethyl acetate-hexane mixture as the eluent. The major frac-25 tion isolated from the column contained 2.01 9 (91~) of 4-(t-butoxycarbonyl)acetophenone as a white solid: mp 56.5-57.5C; NMR (CDC13 80 MHz) d 1.61 (s, 9H), 2.63 (s, 3H, 7.95 (d, 2H, J=9.0 Hz), 8.09 (d, 2H, J=9.0 Hz); IR
(KBr) 2980, 2930, 1720, 1680, 1400, 1365, 1295, 1250, 30 1165, 1100, 845, 760 and 690 cm 1.

Alternatively, 4-(t-butoxycarbonyl)aceto-phenone can be isolated by distillation under reduced pressure, bp 90-100C/0.1 mm. Analogously 4-(t-butoxy-~j 4 1 carbonyl)propiophenone is prepared and converted to 3-cyano-2-(4-nitrophenylthio)-5-[2-(4-tert-butoxy-carbonylphenyl)but l-enyl]pyridine.

Exam~le_3 2-A~ino-3-cyano-5-12-(4-ethoxycarbonylphenyl)-ethenyl]pyridine.

A suspension of 2.00 g (4.64 mmol) of 3-cyano-2-(4-nitrophenylthio)-5-12-(4-ethoxycarbonylphenyl), ethenyl]pyridine, 1.~53 9 (6.95 mmol) of cupric bromide, and 50 mL of liquid ammonia was stirred in a pressure tube at room temperature for 13 days. Evaporation of the ammonia afforded a dark residue which waC chromato-graphed over magnesium silicate using methylene chloride as eluant. The eluate was removed by evaporation under reduced pressure and the residue chromatographed on silica gel. Unreacted starting material was eluted with benzene while 0.87 9 (64%) of the product, which can be alternatively named as 2-amino-3-cyano-5-(4-ethoxy-carbonylstyryl)pyridine, was eluted with ethyl acetate and obtained by evaporation of the ethyl acetate solvent as a light yellow solid, mp 135-141.5~C; NMR (Me2SO-d6) delta 1.39 (t, 3H, J-6.3Hz), 4.38 (q, 2H, J=6.3 Hz), 6.67 (m, 2H), 7.10 (br, 2H), 7.45 (d, 2H, J=9 Hz), 7.71 (d, lH, J=3.6 Hz), 7.97 (d, 2H, J=9 Hz), 8.11 (d, lH, 25 J=3.6 Hz); IR (KBr) 3155, 2218, 1715, 1650-1645, 1593, 1491, 1277, 1100 cm 1.

Anal. Calc'd. for C17H15H3O2 5.16; N, 14.33. Found: C, 69.37; H, 5.25; N, 14.22.

By substituting an equivalent amount of 3-30 cyano-2-(4-nitrophenylthio)-5-[2-(4-tert-butoxycarbonyl-phenyl)ethenyl]pyrldine in the foregoing procedure there is obtained 2-amino-3-cyano-5-[2-(4-tert-butoxycarbonyl-1 ' `~4 1 1 phenyl)ethenyl~pyridine, which can be alternatively named as 2-amino-3-cyano-5-(4-t-butoxycarbonylstyryl)-pyridine; yield 1.14 9 (84%) of light yellow crystals, mp 190-195C; NMR (Me2SO-d6) delta 1.57 (s, 9H), 6.57-6.60 (m, 2H), 7.00 ~br, 2H), 7.35 (d, 2H, J=8.1 Hz), 7.65 (d, lH, J=2.7 Hz), 7.84 (d, 2H, J=8.1 Hæ), 8.00 (d, lH, J=2.7 Hz); IR (KBr) 3460, 3360, 2215, 1707, 1623, 1480, 1300, 1287, 1158 cm 1.

Anal. Calc'd. for ClgHlgN3O2: C, 71.00; H, 5.96; N, 13.07. Found: C, 70.B3; H, 6.03; N, 12.83.

Example 4 2,4-Diamino-6-t2-(4-tert-butoxycarbonyl-phenyl)ethenyl]pyrido[2,3-d]pyrimidine.

To a solution of 4.54 mmol of guanidine as the free base (obtained from 0.433 g (4.54 mmol) of guani-dine hydrochloride and 0.114 9 of sodium in 25 mL of dry tert-butanol) was added 1.325 9 (4.12 mmol) of 2-amino-3-cyano-5-[2-(4-tert-butoxyphenyl)ethenyl]pyridine. The deep red suspension was heated at reflux under dry nitrogen for 8 hours. The reaction mixture was cooled to room temperature and filtered. The precipitate was washed successively with water, acetone, and ether and was then dried under reduced pressure to yield 0.911 9 (61%) of the title compound, which can be alternatively 25 named as 2,4-diamino-6~(4-tert-butoxycarbonylstyryl)-5-deazapteridine, as a light yellow solid, mp >350C; NMR
(Me2SO-d6) delta 1.55 (s, 9H), 6.42 (br, 2H), 6.73 (m, 2H), 7.30-8.00 (br, 2H), 7.35 (d, 2H, J=9 Hz), 7.Bl (d, 2H, J=9 Hz), 8.34 (m, 2H); IR (KBr) 3320-3300, 3200-30 3140, 2970, 1718, 1626, 1610-1600, 1550, 1450-1445, 1288, 1167, 812 cm 1.

~3~'..'.4 1 ~

Anal.: Calc'd. for C20~21 5 2 S.82; N, 19.27. F~und: C, 65.88; H, 5.86; N, 18.98.

Example 5 2,4-Diamino-6-l2-(4-carboxyphenyl)ethenyl]-pyrido[2,3-d]pyrimidine.

A. A solution of 1.27 9 of 2,4-diamino-6-[2-(4-tert-butoxycarbonylphenyl)ethenyl]pyrido[2,3-d]-pyrimidine and 10 mL 88% formic acid was stirred at room temperature. A yellow solid started to form after about 12 hours and after 4 days of stirring, the reaction mix-ture was filtered. The collected solid was washed well successively with water, methanol, and acetone and was then dried under reduced pressure to give 0.85 9 (79%) of the title compound, which can be alternatively named as 2,4-diamino-6-(4-carboxystyryl) 5-deazapteridine, mp >300C.

B. Alternatively, 0.48 9 of 2,4-diamino-6-[2-(4-tert-butoxycarbonylphenyl)ethenyl]pyridol2,3-d]-pyrimidine was added to a saturated solution of hydrogen chloride in 20 mL of nitromethane at 0. The reaction mixture quickly became viscous and turned a deep yellow color and after a few minutes of stirring, a granular solid formed. After 1 hour of stirring, 50 mL of ether were added and the precipitate was collected by filtra-2S tion. The collected solid was dissolved in 50 mL of 10%aqueous sodium carbonate. Acidification with acetic acid then resulted in the separation of a yellow solid which was collected by filtration and dried under reduced pressure; yield 0.31 g (92%) of 2,4-diamino-6-[2-(4-carboxyphenyl)ethenyl]pyrido[2,3-d]pyrimidine; NMR
(Me2SO-d6) delta 6.75 (s, 2H), 7.35, 7.85 (AB q, 4H, J=9 Hz~, 8.38 (s, 2H); IR (Nujol) 3400-2300, 3380, 31S0, 1700, 1650, 1630, lS90 cm 1.
* Trademark 1 3 Q~4 1 1 Example 6 2,4-Diamino-6-l2-(4-tert-butoxycarbonyl-phenyl)prop-l-enyl]pyrido~2,3-d]pyrimidine.

To a suspension containing 1.93 9 of guanidine hydrochloride in 75 mL of dry tert-butanol at 50C under a nitrogen atmosphere was added 0.50 9 of sodium metal.
~fter all the sodium was dissolved, 7.97 9 o~ trans-3-cyano-2-(4-nitrophenylthio)-5-[2-(4-tert-butoxycarbonyl-phen~l)prop-l-enyl]pyridine was added. The mixture was heated under reflux for 3 hours, cooled to room tempera-ture, diluted with ether, and filtered. The solid was washed with water and acetone, and then dried under re-duced pressure to yield 4.66 9 (73%) of the title com-pound as a pale yellowish solid; mp >300C; NMR
15 (DMSO-d6, 80 MHz) d 1.56(s, 9H), 2.23 and 2.29 (brs, 3~), 6.57 and 6.99 (brs, lH), 7.25-8.73 (m, 8H); IR
(KBr) 3340, 3130, 1710, 1640, 1608, 1540, 1450, 1365, 1340, 1290, 1165, 1110, 840 and 810 cm 1.

Example 7 2,4-Diamino-6~[2-(4-carboxyphenyl)prop-1-enyl~pyrido[2,3-d]pyrimidine.

A suspension containing 4.58 9 of 2,4-diamino-6-[2-(4-tert-butoxycarbonylphenyl)prop-1-enyl]pyrido-[2,3-d]pyrimidine in 200 mL of a saturated solution of hydrogen chloride gas in nitromethane was stirred at 0C
for 1 hour, and then at room temperature for 3 hours.
After dilution with ether, the reaction mixture was filtered and the collected solid was washed successively with water, methanol, and acetone and then dried under 30 reduced pressure to give 3.90 g (100%) of 2,4-diamino-6-[2-~4-carboxyphenyl)prop-1-enyl]pyridol2,3-d]pyrimidine.
NMR (DMSO-d6, 80 MHz) d 2.31 (brs, 3H), 6.77 and 7.07 I ~`"3~ 1 1 (brs, lH), 7.74 (d, 2H, J=8.5Hz), 7.98 (d, 2H, J=8.5 Hz), 8.26, (d, lH, J=2.0 Hz), 8.74 d, lH, J=2.0Hz).

Example 8 2-Amino-4 hydroxy-6-[2-(4-carboxyphenyl)-ethenyl]pyrido[2,3-d]pyrimidine.

A. A suspension of 1~0 9 of 2,4-diamino-6-l2-(4-carboxyphenyl)ethenyl]pyrido[2,3-d]pyrimidine in 30 mL of 1 N aq~eous sodium hydroxide was heated under reflux under nitrogen for 3 hours. The resulting homo-genous orange solution was cooled to room temperature, acidified with 6 mL of glacial acetic acid, and the resulting yellow precipitate collected by filtration.
The filter cake was washed successively with water, me~hanol, acetone and ether and was then dried under reduced pressure to give 0.88 9 (88%~ of the title compound, which can be alternatively named as either 6-l2-(4-carboxyphenyl)ethenyl]-5-deazapterin or 6-(4-carboxystyryl)-5-deazapterin, as a microcrystalline yellow powder, mp >250C; NMR (TFA-dl delta 6.8, 7.25 (AB q, 2H, J=12 Hz), 7.45, 8.2 (AB q, 4H, J=9 Hz), 8.55 (s, lH), 8.85 (s, lH); IR (Nujol) 3500-2500 (br), 1670, 1625, 1600 cm B. In a similar fashion, 2,4-diamino-6-[2-(4-carboxyphenyl)prop-1-enyl3pyrido[2,3-d]pyrimidine is converted to 2-amino-4-hydroxy-6-12-(4-carboxyphenyl)-prop-l-enyl]pyrido[2~3-d]pyrimidine, mp >250C; NMR
(DMSO-d6, 80 MHz) d 2.28 and 2.30 (brs, 3H), 6.77 and 7.06 (brs, lH), 7.72 (d, 2H, J=8.5Hz), 7.97 (d, 2H, J=8.5 Hz), 8.27 (d, lH, J=2.0 Hz), 8.72 (d, lH, J=2.0 Hz).

, 4 1 1 Example 9 2-Acetamido-4-hydroxy-6-[2-(4-acetoxycarbonyl-phenyl)ethenyl]pyrido[2,3-d~pyrimidine.

A suspension of 0.88 9 of 2-amino-4-hydroxy-6-[2-l4-carboxyphenyl)ethenyl]pyrido~2~3-d]pyrimidine in 20 mL of acetic anhydride containing 0.05 9 of 4-di-methylaminopyridine was heated under nitrogen at 120C
for 3 hours. The reaction mixture was cooled to room temperature. Fifty milliliters of ether were added and the resulting yellow solid was collected by filtration to yield 0.95 9 (84%) of the title compound; mp >300C;
IR (Nujol) 3350, 3150, 1800, 1670, 1600 cm Example 10 2-Acetamido-4-hydroxy-6-[2-(4-carboxyphenyl)-ethenyl]pyrido[2,3-d]pyrimidine.

To a suspension of 0.95 9 of 2-acetamido-4-hydroxy-6-12-(4-acetoxycarbonylphenyl)ethenyl]pyrido-[2,3-d]pyrimidine in 50 mL of water was added 1 N
aqueous sodium hydroxide until a homogenous solution was obtained. Acidification with acetic acid resulted in the formation of a yellow precipitate which was collec-ted by filtration. The filter cake was washed sequen-tially with water, methanol, acetone and ether. The residual solid was recrystallized from DMF to ~ive 0.65 9 (77%) of the title compound, which can be alterna-tively named as 2-acetamido-6-(4-carboxystyryl)-5-deaza-4(3H)-pteridinone, as a microcrystalline yellow solid, mp >300C; NMR ~FA-dl) delta 2.5 (s, 3H), 6.85, 7.32 (AB q, 2H, J=12 Hz), 7.45, 8.18 (RB q, 4H, J=9Hz), 8.65 (s, lH), 9.02 ts, lH); IR (Nujol) 3300-2200 (br), 1685, 1655, 1630, 1600, 1565 cm . MS: Calc'd. for C18H14N4O4: 350. Fo~nd: m/e 350 (base), 308.

l ~jn~41 1 Example 11 2-Acetamido-4-hydroxy-6-[2-(4-carboxyphenyl)-prop-l-enyl]pyridol2,3-d]pyrimidine.

By subjecting 2-amino-4-hydroxy-6-12-(4-carboxyphenyl)prop-1-enyl]pyridol2,3-d~pyrimidine to the procedures of Examples 9 and 10, there was obtained 2-acetamido-4-hydroxy-6-~2-(4-carboxyphenyl)prop-1-enyl]pyrido[2,3-d]pyrimidine, mp >250C; in an overall yield of 45%; NMR (CF3CO2D/DMSO-d6, 80 mHz) d 2.15 (s, 10 3H), 2.22 (s, 3H), 6.72 (brs, lH), 7.45 (d, 2H, J=8.4 Hz), 7.92 (d, 2H, J=8.4 Hz), 8.65 (d, lH, J=2.G Hz), 8.98 (d, lH, J=2.0 Hz).

Example 12 Diethyl N-(4-[2-(2,4-diaminopyridol2,3-d]-pyrimidin-6-yl)ethenyl]benzoyl)-L-glutamate.

A. To a solution of 1.0 9 (0.0033 mol) of 2,4-diamino-6-~2-(4-carboxyphenyl)ethenyl~pyrido[2,3-d]-pyrimidine and 1 g of N-methylmorpholine in 120 mL of N-methylpyrrolidone cooled to 5C is added, in a drop-20 wise fashion, 1.4 9 (0.0048 mol) of diphenyl chloro-phosphonate. The reaction mixture was stirred for 1 hour and an additional 0.5 mL of N-methylmorpholine were added, followed by 1.1 g (0.0048 mol) of diethyl L-glutamate hydrochloride. The reaction mixture was stirred overnight at room temperature and the solvent then was removed under reduced pressure. The residual solid was washed with S0 mL of dry ether, triturated with 100 mL of 1 N aqueous sodium hydroxide, and the resulting suspension centrifuged. The collected solid 30 was dissolved in 200 mL of 3:1 chloroform:methanol and filtered through Florisil. The filtrate was evaporated to a small volume; 10 g of Florisil were added, and the * Trademark 1, ''~1 1 resulting impregnated Florisil added to the top of a --Florisil column which was then eluted sequentially with ---ethyl acetate followed by ethyl acetate containing increasing quantities of methanol ~9:1, 3:1, and 1:1).
The title compound was collected in the 3:1 and 1:1 fractions. Evapora~ion of the combined eluates gave a glassy material which was triturated with ether and then collected by filtration; yield 0.41 9 (26%), mp 183-185C; NMR (Me2SO-d6/TFA~ delta 1.25-1.45 (over-lapping t, 6H, J=7 Hz), 2.25-2.50 (m, 2H), 2.5-2.8 (m, 2H), 4.05-4.45 (overlapping qf 4H, J=7 ~z), 4.8-5.0 (m, lH), 6.8, 7.2 (AB q, 2H, J=16 Hz), 7.4, 7.8S (AB q, 4H, J=9 Hz), 8.6 (s, lH), 9.05 (s, lH); IR (Nujol) 3500-3000, 1730, 1635, 1605 cm . MS: Calc'd. for C25H28N6O5: 4g2. Found: m~e 492, 290, 94, 84.

B. Alternatively the triphenylphosphonium salt [prepared from triphenylphosphine and diethyl 4-bromomethylbenzoylglutamate (7.86 9, 0.012 mol) following the method of Yan et al., J. Het. Chem., 16, 541 (1979)] was added portionwise to a slurry of 0.4 g (0.01 mol) of sodium hydride (60~ suspension in oil) in 70 mL of dry N-methylpyrrolidone over a period of 10 minutes. The resulting red reaction mixture was stirred at room temperature under nitrogen for 1 hour. To this ln situ Wittig reagent were added 2.27 ~ (0.012 mol) of 2,4-diamino-6-formylpyrido[2,3-d]pyrimidine [prepared by the method of Baldwin et al., J. Org. Chem. 43, 2529 (1978)]. The resulting slurry was stirred at room temperature under nitrogen for 3 weeks. The solvent was then evaporated under reduced pressure, the re idual solid triturated with benzene to remove triphenyl-phosphine oxide, and the purified solid collected by centrifugation. The solid was resuspended in water, filtered, and the collected solid dissolved in 200 mL of chloroform:methanol (1:2). Florisil (10 9) was added, the mixture was evaporated to dryness, and the impreg-_ nated Florisil residue applied to the top of a Plorisil column which w~s then eluted with ethyl acetate con-taining inoreasing guantities of methanol tfrom 9:1 to 1:1). Fractions containing eluted material were com-bined and were shown to contain two products (TLC). Themixture was chromatographed ~gain on ~ilica gel util-izing chloroform and methanol as eluants. The initial fraction was a phosphorane and the product was there-after eluted and obtained in a yield of 1.7 9 ~34.5%) in form identical to that obtained in par~ A of this ex-ample.

C. Following the pro~edure of part A of this example but utilizing 2,4-diamino-6-12-(4-carboxy-phenyl)prop-l-enyl]pyridol2,3-d~pyrimidine, there can be obtained diethyl N-(4-l1-(2,4-diaminopyridol2,3-d]-pyrimidin-6-yl)prope~-~-yl]benzovl)-L-~lutamate.

Alternatively, 2.2 9 (0.0074 mol) of di-tert-butyl L-glutamate hydrochloride were allowed to react with 1.5 9 (0.0049 mol) of 2,4-diamino-6-[2-(4-carboxy-phenyl)ethenyl]pyridol2,3-dlpyrimidine, to yield di-tert-butyl N-(4-l2-(2,4-diaminopyridol2,3-d]-pyrimidin-6-yl)ethenyl]benzoyl)-L-glu~amate in a yield of 1.3 9 ~48%), mp >300C. NMR (CDC13/CD30D) delta 1.47, 1.52 (2s, 18H), 2.0-2.6 (m, 4H), 4.5-7.0 (m, lH), 6.8 (br, s, 2H), 7.35, 7.78 (AB q, 4H, J~9 Hz), 8.38 (s, lH), 8.5 (s, 1~); IR (Nujol) 3350, 3180, 1725, 1640, 1605 cm . MS: Calc'd.: for C29H36N6O5 548. Found m/e 548, 446/ 290.

Exam~le 13 Diethyl N-(4-l2-(2-acetamido-4-hydroxypyrido-12,3-djpyrimidin-6-yl)ethenyl]benzoyl)-L-glutamate.

To an ice cold solution of 1.5 9 ~0.0043 m~l) of 2-acetamido-4-hydroxy-6-12-14-carboxyphenyl)ethenyl]-pyridol2,3-d]pyrimidine in 40 mL of N-methylpyrrolidone containing l.S mL of N-methylmorpholine was added 1.72 9 ~0.0064 mol~ of phenyl N-phenylpho~phoramidochloridate in a single portion; The resulting mixture was stirred at 0C for 30 minutes. Diethyl L-glutamate hydro-chloride (1.53 9, 0.0064 mol) was then added and the reaction mixture was stirred at room temperature over-night. The sulvent was evaporated under reduced pres-sure and the residual ~olid triturated with 50 mL of 1 N aqueous odium carbonate. The mixture was filtered and the collected ~olid dissolved in 20 mL of chloro-form. The chloroform solution was dried over ~nhydrous lS magnesium sulfate and filtered. The filtrate was evap-orated to dryness and chromatographed on ~ilica gel.
Elution with chloroform:methanol ~95:5) gave 1. 5? 9 ~66~) of the title compound, which may be alternatively named as diethyl 2-acetyl-5,10-dideaza~9,10-didehydro-folate, mp >250C; NMR ~CDC13Me2SO-d6) delta 1.15-1.45 (2t, 6H, J~6 Bz), 2.0-2.65 (m, 4H), 2.3 (s, 3H), 4.0-4.35 (2g, 4H, J~6 Hz), 4.5-4.75 (m, lH), 6.7, 6.9 (AB q, 2H, J~15 Hz), 7.33, 7.84 (AB q, 4H, J~9 Hz), 8.25-8.3B (m, 2H), 3.62 ~d, lH, J~2Hz)~ 11.5-12.5 (br, 2H); IR ~Nu jol) 3320, 3150, 1730, 1680, 1630, 1600 cm Anal.: Calc'd. for C27H29N5O~: C, 60.56; H, 5.42; N, 13.08. Found: C, 60.26; H, S.45; N, 12.84.

Example 14 Diethyl N-(4-[~-(2-acetamido-4-hydroxypyrido-[2,3-d]pyrimidin-6-yl)propen-2-yl~benzoyl)-L-gluta~ate.

~ o a solution of 0,2 g of 2-acetamido-4-hydroxy 6-l2-(4-carboxyphenyl)prop-1-enyl]pyridol2,3-d]-pyrimidine in 50 mL of N-methylpyrrolidinone containing 1 3 n 3 ~ 1 1 0.18 9 of N-methylmorpholine was added 0.22 9 of phenyl N-phenylphosphoramidochloridate in a single portion.
After stirring the mixture at room temperature for 1 hour, 0.20 9 of diethyl L-glutamate was added. The reaction mixture was stirred overnight, the solvent was removed under reduced pressure and the residue was triturated with chloroform. The mixture was filtered and the filtrate was evaporated under reduced pressure.
The residue was subjected to preparative thin layer chromatography on silica gel using a 5% methanol in chloroform mixture as the eluent. This gave 74.6 mg (2S%) of the title compound as a pale yellowish solid;
NMR tCDC13, 250 MHz) d 1.22 (t, 3H, J=7.1 Hz), 1.30 (t, 3H J=7.1 Hz), 2.11-2.57 (m, lOH), 4.14 (q, 2H, J=7.1 15 Hz), 4.24 (q, 2H, J=7.1 Hz), 4.75-4.83 (m, lH), 6.86 (brs, lH), 7.18 (brs, lH), 7.57 (d, 2H, J=8.42 Hz), 7.84 ld, 2H, J=8.42 Hz), 8.50 (d, lH, J=2.01 Hz), 8.96 (brs, lH), 10.34 (brs, lH).

Example 15 Diethyl N-(4-[2-(2,4-diaminopyrido[2,3-d]-pyrimidin-6-yl)ethyl3benzoyl)-L-glutamate.

A solution of 0.9 9 of diethyl N-(4-12-(2,4-diaminopyrido[2,3-d]pyrimidin-6-yl)ethenyl]benzoyl)-L-glutamate in 40 mL of trifluoroacetic acid was hydro-25 genated under 55 psi of hydrogen for 24 hours using ~.5 g of Pd/C as catalyst. The catalyst was removed by fil-tration through celite and the filtrate was evaporated.
The residual solid was triturated with 30 mL of 2 N
aqueous sodium carbonate, followed by a water wash. The resulting solid was purified by column chromatography on silica gel. Elution with chloroform:methanol (95:5) afforded a small amount (0.2 9) of the tetrahydro deriv-ative while subsequent elution with chloroform:methanol (1:4) gave 0.52 9 (58%) of the title compound, which can 1 7 ~3~11 be alternatively named as diethyl N-[4-[2-(2,4-diamino-5-deaza-6-pteridyl)ethyl]benzoyl]-L-glutamate; mp >200~C;
NMR ~Me2SO-d6) delta 1.1-1.3 ~2t, 6H, J=7 Hz) 1.8-2.6 (m, 4H), 3~05 (s, 4H), 3.1-3.8 (br, 5H), 3.9-4.2 (2q, 4H, J=7 Hz), 4.3-4.5 (m, lH), 7.35, 7.85 (AB q, 4H, J=9 Hz), 8.6 (br, s, 2H): IR (Nujol) 3320, 3150, 1650 cm 1.

Example 16 Diethyl N-(4-~2-(2,4-diamino-5,6,7,8-tetra-pyrido[2,3-d]pyrimidin-6-yl)ethyl]benzoyl~-L-glutamate.

By repeating the procedure of Example 15 but continuing the hydrogenation for 72 hours, the title compound, which can be alternatively named as diethyl N-(4-12-(2,4-diamino-5-deaza-5,6,7,8-tetrahydro-6-pteridyl)ethyl]-benzoyl)-L-glutamate, was obtained as a crude product which was chromatographed on silica gel using chloroform:methanol (95:5) to give 0.42 9 (31%) of the product as a colorless microcrystalline ~olid; mp >250C; NMR (Me2SO-d6) delta 1.6, 1.8 (2t, 6H, J=6 Hz), 1.4-3.8 (m, 13H), 4.1 (2q, 4H, J=6 Hz), 4.3-4.6 (m, lH), 20 6.8 (s, 2H), 7.35, 7.85 (AB q, 4H, J=9Hz), 8.7 (d, lH, J=9 Hz); IR (Nujol) 3350, 3150, 1730, 1630 cm . MS:
Calc'd. for C25H34N6O5: 498. Found: m/e 498, 425, 178, 165 (base), 150.

Example 17 Diethyl N-(4-[2-(2-acetamido-4-hydroxy-5,6,7,8-tetrahydropyrido[2,3-d]pyrimidin-6-yl)ethyl]-benzoyl)-L-glutamate.

A solution of diethyl N-(4-[2-(2-acetamido-4-hydroxypyrido[2,3-d]pyrimidin-6-yl)ethenyl]benzoyl~-L-glutamate in 30 mL of trifluoroacetic acid was hydro-genated at 55 psi of hydrogen in the presence of 1.0 9 "`34 1 1 of 5% Pd/C ~t room temperature ~or 14 hours. The c~talyst was removed by filtration, the filtr~te evap-orated under reduced pre88ure~ and the residual solid partitioned between 100 mL of chloroform and 50 ml of 2N
~queous ~odium carbonate. The organic ph2se was 6epara-ted, dried over anhydrou~ magne~ium sulfate, and the solvent removed by evaporation to give ~ gum which was chromatographed on silica gel. Elution with chloroform:methanol t97:3~ gave 0.25 9 (56~) of diethyl N-~4-l2-(2-acet~mido-4-hydroxypyridol2,3-d]pyrimidin-6-yl)ethyl]benzoyl)-L-glutamate; mp 215-217C; NMR
(CDC13) delta 1.25, 1.35 ~2t, 6H, J~6 Hz), 2.1-2.5 (m, 4H), 2.55 (~, 3H), 3.1 (s, 4H), 4~15, 4.25 (2g, 4H, J-6 Hz), 4.6-4~96 (m, lH), 7.05 (8, lH), 7.25, 7.75 (AB q, 4H, J~9 Hz), 8.35 ~d, lH, J~3 Hz), 8.77 (d, lH, J~3 Hz);
IR (Nujol) 3200, 3150, 1725, 1675, 1630, 1605 cm 1.
Anal.: Calc'd. for C27H31N5O7: C, 60.32; H, 5.81; N, 13.03. Found: C, 59.98; H, 6.03; N, 12.92).

Further elution with 95:5 chloroform:methanol yielded 0.08 9 (18~) of the title compound, which can be alternatively named ~s diethyl 2-acetyl-5,10-dideaza-5,6,7,B-tetrahydrofolate; mp>200C; NMR (CDC13/Me2SO-d6) delta 1.24, 1.28 ~2t, 6H, J~6 Hz), 1.5-3.3 (m, 13H), 2.18 (5, 3H), 4.1, 4.1B (2a, ~H, J~6 Hz), 4.4-4.7 (m, lH), 6.2 (s, lH), 7.28, 7.85 (A3 q, 4H, J~9 Hz), 8.4 (d, lH, J~8 Hz); I~ (Nujol) 3320, 3250, 1730, 1630, 1575 cm 1. Anal.: Calc'd. for C27H35N5O~: C, 59.87; H, 6.51;
N, 12.93. Found: C, 59.66; H, 6.71; N, 12.77.

Example 18 Diethyl N-(4-11-(2-acetamido-4-hydroxy-5,6,7,8-tetrAhydropyrido[2,3-d]pyrimidin-6-vl)prop-8-yl3-benzoyl)-L-glutamate.

A ~olution of 84.4 mg of diethyl N-(4-12-(2-acetamido-4-hydroxypyridol2,3-d]pyrimidin-6-yl)-, 4 1 1 prop-l-enyl]benzoyl)-L-glutamate in 30 mL of trifluoro-acetic acid was hydrogenated at 55 psi of hydrogen in the presence of 0.42 9 of 5~ Pd/C at room temperature for 24 hours. The catalyst was removed by filtration and the filtrate was evaporated under reduced pressure.
The resulting residue was taken up in chloroform and was extracted with a saturated sodium bicarbonate solution.
The organic layer was dried over anhydrous sodium sul-fate and the solvent was removed under reduced pressure.
The residue was then subjected to thin layer chromato-graphy using a 5~ methanol:chloroform mixture as the eluent. After elution of a first fraction, there was obtained 19.6 mg of the title compound, NMR (CDC13 250 MHz)j d 1.20-1.33 (m, overlapping methyls, 9H), 2.45-3.36 ~m, lSH), 4.11 (q, 2H, J=7.14 Hz), 4.23 (q, 2H, J=7.10 Hz), 4.89 (m, lH), 5.44 (brs, lH), 7.24 (d, 2H, J=7.54 Hz), 7.72 (d, 2H, J=7.54 Hz), 9.77 (brs, lH), 11.26 (brs, lH).

Example 19 ~-(4-l2-(2,4-diaminopyridol2,3-d]pyrimidin-6-yl)ethyl]benzoyl)-L-glutamic acid.

A solution of 0.38 9 of diethyl N-(4-12-(2,4-diaminopyridol2,3-d~pyrimidin-6-yl)ethyl]benzoyl)-L-glutamate in 50 mL of methanol containing 4.6 mL of 0.5 N aqueous sodium hydroxide was stirred at room tempera-ture for 72 hours. Acetic acid (5 mL) was addedl and the resulting white precipitate collected by filtration.
The filter cake was washed well with water, methanol, and ether and was dried under reduced pressure to yield 0.15 9 (44~) of the title compound, which can be alter-natively named as 5,10-dideazaaminopterin; mp >250C;
NMR (TFA-dl) delta 2.2-2.7 (m, 2H), 2.28; 2.7-2.95 (m, 2H), 5.0-5.2 (m, lH), 7.35 and 7.85 (AB q, 4H, J=9 Hz), 8.7 (s, lH), 9.1 (s, lH).

Example 20 N-(4-[2-(2,4-di~mino~5,6,7,8-tetrahydropyrido-2~3-d]pyrimidin-6-yl)ethyl)benzoyl)-L-glutamic acid.

Following the procedure of Example 19, S hydrolysis of 0.35 9 of diethyl N-(4-12-(2,4-diamino-5,6,7,8-tetrahydropyrido[2,3-d]pyrimidin-6-yl)ethyl]-benzoyl)-L-glutamate yielded 0.13 9 (42%) of the title compound, which can be alternatively named as 5,10-dideaza-5,6,7,8-tetrahydroaminopterin, mp >250C.

Example 21 N-(4-[2-(2-amino-4-hydroxypyrido~2,3-d~-pyrimidin-6-yl)ethyllbenzoyl)-L-glutamic acid.

A homogeneous solution of 0.175 of diethyl N-(4-[2-(2-acetamido-4-hydroxypyrido[2,3-d]pyrimidin-6-yl)ethyl~benzoyl)-L-glutamate in 50 mL of methanol containing 3 mL of lN aqueous sodium hydroxide was stirred at room temperat~re for 72 hours. Addition of 2 mL of acetic acid followed by centrifugation gave 0.125 9 (86%) of the title compound, which can be alterna-tively named as 5,10-dideazafolic acid, as a micro-crystalline colorless solid, mp >200C; NMR (TFA-dl) delta 2.3-2.7 (m, 2H), 2.7-3.0 tm, 2H), 3.25 (s, 5H), 4.9-5.25 tm, IH), 7.35, 7.85 (AB q, 4H, J=9 Hz), 8.50 (s, lH), 8.90 (s, lH).

Example 22 N-(4 [2-(2-amino-4-hydroxy-5,6,7,8-tetra-hydropyrido[2,3-d]pyrmidin-6-yl)ethyl]benzoyl)-L-glutamic acid.

1 3 3 4 ¦ I ) Diethyl N-(4-12-(2-acetamido-~-hydroxy-5,6,7,8-tetrahydropyridot2,3-d]pyrimidin-6-yl)ethyl) benzoyl)-L-glutamate was hydrolyzed in an flnalogous f~shion to that described in Example 21 to yield the ~itle compound, which can be alternatively named ~s 5,10-dideaza-5~6,7,8-tetrahydrofolic acid, in 87~ yield;
mp >250C, NMR (TFA) delta 1.7-3.9 (m, 13H), 5.0-5.25 (m, lH), 7.45, 7.85 ~AB q, 4H, J~9 Hz).

Similarly obtained from diethyl N-(4-12-10 (2-acetamido-4-hydroxypyrido[2,3-d]pyrimidin-6-yl)-ethenyl]benzoyl)-L-glutamate was N~ 2-(2-amino-4-hydroxypyrido(2,3-d]pyrimidin-6-yl)ethenyl]benzoyl)-L-glutamic acid, mp ~200C.
.

2xample 23 N-~4-11-t2-amino-4-hydroxy-5,6,7,8-tetra-hydr~pyrido[2~3-d]pyrimidin-6-yl~prop-2-yl~benzoyl)-L-glutamic acid.

A homogeneous solution of 17.5 mg of diethyl N-(4-1].-~2-acetamido-4-hydroxy-5,6,7,8-tetrahydropyrido_ 12,3-d]pyrimidin-6-yl) ~r~p-2-yl]benæ~y1)-L-~luta~ate in 2 mL of methanolic ~odium hydroxide ~olution was allowed to ~tand at room temperature for 72 hours. Most of the solvent was then removed under reduced pressure and the mixture was diluted with water ~nd ~cidified with acetic a~id. The precipitate was collected by filtration, washed with wa~er, and dried under reduced pressure (0.1 mm) for ~ hours to give 9.7 mg (67%) of the title com-pound, which may be alternatively named as 5,10-dideaza-10-methyl-5,6,7,8-tetrahydrofolic acid. mp >250~C, NMR
30 delta 0.87-O.B8 (br~, lH each), 1-2.8 (m, 11H), 3.13 (m, 1H), 4.59 (m, lH), 6.96 (d, 2H, J-9 Hz), 7.34 ~d, 2H, J~9 Hz.

1 7r'3~1 1 Analogously N-(4-[1-(2-amino-4-hydroxy-5,6,7,8-tetrahydropyrido[2,3-d]pyrimidin-6-yl)but-2-yll-benzoyl)-L-glutamic acid and N-(4-[1-(2,4-diamino-5,6,7,8-tetrahydropyrido[2,3-d]pyrimidin-6-yl~but-2-yl~-S benzoyl)-L-glutamic acid are prepared~

In typical models, the indicated tumor cells were implanted subcutaneously in the axillary region of mice. Following intraperitoneal administration of the first compound of Example 22, the length and width of the control tumor (receiving only saline) are measured at the indicated time and compared to those of animals receiving the test compound to calculate percentage of inhibition.

% INHIBITION
Dose mg/kg Days of Tumor System 25 50 100 200 Treatment Lymphosarcoma91 100 100 100 8 C3H Mammary Adenocarcinoma 86 100 100 1.00 lQ
Lewis Lung Carcinoma 58 77 94 100 10 M-5 Ovarian *
Carcinoma 12 31 54 80 10 Madison Lung 54 72 87 90 10 X5563 Plasma Cell Myeloma 100100 100 100t 10 * 5 Day Delay t Toxic

Claims

The embodiments of the invention in which an exclusive property or privilege is claimed are defined as follows:

1. A compound of the formula:

wherein R1 is amino or hydroxy;
R3 is hydrogen, methyl, or ethyl; and Z is hydroxy, (lower) alkoxy, or -NH-C*(COOX)-CH2CH2COOX in which X is hydrogen or (lower) alkyl and the configuration about the carbon atom designated * is L.