CA1299577C - Tetrahydrocarbazole 1-alkanoic acids - Google Patents

Abstract

TITLE OF THE INVENTION

ABSTRACT OF THE DISCLOSURE
Tetrahydrocarbazole 1-alkanoic acids are disclosed. The compounds act as prostaglandin and thromboxane antagonists and are useful in treating asthma, diarrhea, hypertension, angina, platelet aggregation, cerebral spasm, premature labor, spontaneous abortion and dysmenorrhea and as cytoprotective agents, and have the following general formula:

I

wherein:
R1, R2, R3, R4, R5 and R6 are each independently selected from:
(1) hydrogen;
(2) alkyl having 1 to 6 carbon atoms;
(3) alkenyl having 2 to 6 carbon atoms;
(4) -(CH2)nM
wherein n is O to 3 and M is a) OR13;
b) halogen;
c) CF3;
d) SR13;
e) phenyl or substituted phenyl wherein substituted phenyl i s as defined below in the definition of R13;

f) COOR14;
g) -?-R15;
h) -NH-?-R16 wherein R16 is C1 to C6 alkyl, benzyl or phenyl;
i) -NR14R14;
j) -NHSO2R17 wherein R17 is C1 to C6 alkyl, 4-methylphenyl, phenyl, or CF3;
k) -?-CH2OH;
l) -SOR13;
CONR14R14;
n) -SO2NR14R14;
o) -SO2R13;
p) NO2 ;
q) -O-?-R15;
r) -O-?-NR14R14;
s) - O-?-OR16;
t) CN;
u) N3;
R7 is H or alkyl of 1 to 6 carbons;
R8 is H or alkyl of 1 to 6 carbon atoms;
each R9 is independently H, OH, C1 to C4-O-alkyl or alkyl of 1 to 4 carbons;

R10 is COOH; CH2OH; CHO;
NHSO2R11 wherein R11 is OH, alkyl or alkoxy of 1 to 6 carbons, perhaloalkyl of 1 to 6 carbons, phenyl or phenyl substituted by alkyl or alkoxy groups of 1 to 3 carbons, halogen, hydroxy, COOH, CN, formyl or acyl to 1 to 6 carbons; CONHSO2R11;
hydroxymethylketone; CN; or CON(R9)2;
r is 1 to 6;
each R13 independently is H; C1 to C6 alkyl; benzyl; phenyl or substituted phenyl wherein the substituents are C1 to C3 alkyl, halogen, CN, CF3, COOR14, CH2COOR14, C1 to C3 alkoxy, or C1 to C4 perfluoroalkyl;
each R14 is independently H, phenyl, benzyl or C1 to C6 alkyl; and, each R15 independently is H, (CH2)mCOOR14 wherein m is 0 to 4, C1 to C6 alkyl, CF3, phenyl, or substituted phenyl wherein substituted phenyl is as defined above in the definition of R13;
or a pharmaceutically acceptable salt thereof.

Description

5012P/5011~ g S~ ~

TITLE OF THE INVENTION
TETRAHYDRQCAR~AZOLE l-ALKANOIC ACIDS

BACKGROUND OF THE INVENTION
This invention relates to prostaglandin antagonists useful in treating a variety of conditions, such as allergic asthma where excessive contractile activity of prostaglandins and prostaglandin biosynthetic intermediates occur.
These compounds antagonize the actions of ; contractile prostaglandins, such as PGF2a, : PGG2, PGH2, PGD2 and TXA2. The use of agents : :
: which act as prostaglandin antagonists offers new : : approaches to therapy in a number of disease states.
; ~ 15 For example, certain prostaglandins, such as PGF2a, PGD2, PGG2, and PGH2, are potent bronchospastic agents. Indeed human asthmatics have been shown to be especially sensitive to the bronchial constricting action of PGF2~.
: 20 : .
;
~ ~ 25 : :
:: :

lZ~95~

The compounds of the present invention are also antithrombotic agents. Thus, they are useful in the treatment and/or prevention of thromboembolic diseases such as arterial thrombosis and those involving platelet deposition, e.g. prothesis.
In addition to the involvement of contractile prostaglandins in asthma, prostaglandins ar~ known to play a role in other allergic conditions, as well as, diarrhea, hypertension, ~ 10 angina, platelet aggregation, cerebral spasm, ; cerebral ischemia, arrythmia, circulatory shock, sudden death, atherosclerosis, myocardial ischemia, premature labor, spontaneous abortion, dysmenorrhea, glomerular nephritis, and systemic lupus erythem-15 atosis. Consequently, the compounds of this invention will alleviate the above mentioned diseases.
In addition to the prostaglandin antagonist actions, the compounds of this invention are inhibitors of the biosynthesis of 5-lipoxygenase 20 metabolites of arachidonic acid, such as 5-HPETE, 5-HETE and the leukotrienes. Leukotrienes B4, C4, D4 and E4 are known to contribute to various disease conditions such as asthma, psoriasis, pain, ulcers and systemic anaphylaxis. Thus 25 inhibition of the synthesis of such compounds will alleviate these and other leukotriene-related disease states.
The compounds of the present invention may be used to treat or prevent mammalian (especially, ; ~ 30 human) disease states such as erosive gastritis;
erosive esophagitis; ethanol-induced hemorrhagic ~ erosions; hepatic ischemia; noxious agent induced '~ ~

,. .

~Z9~S77 damage or necrosis of hepatic, pancreatic, renal, or myocardial tissue; liver parenchymal clamage caused by hepatotoxic agents such as CC14 and D-galactosamine;
ischemic renal failure; disease-induced hepatic damage; bile salt induced pancreatic or gastric damage; trauma- or stress-induced cell damage; and glycerol-induced renal failure.
Certain 9-benzyl-1,2,3,4-tetrahydro-carbazole acetic acids or esters thereof are shown as 10 chemical intermediates in the preparation of carbazoles that are known in the art as anti-inflammatory, analagesic and anti-rheumatic agents (see U.S. Patent 3,896,145 and British Patent 1,385,620). Certain 9-benzyl-1,2,3,4-tetrahydro-15 carbazole carboxylic acids are known in the art asanti-inflammatory, analgesic and anti-rheumatic agents (see U.S. Patents 3,868,387; 4,009,181;
3,905,998 and 3,758,496), and 9-benzyl-carbazole carboxylic acids (U.S. Patents 3,956,295 and 20 4,057,640~ and 9-benzylcarbazole acetic acids and esters thereof (U.S. Patent 3,896,145 and British Patent 1,385,620) are known as anti-inflammatory, analgesic and anti-rheumatic agents. None of these compounds, however, are shown to be prostaglandin, or 25 thromboxane antagonists or inhibitors of leukotrine biosynthesis.
~ .

.

~9~S77 DESCRIPTION OF THE INVENTION
The present invention relates to novel compounds of Formula I:

~ ~ R

(C) -R10 k8-CH R R9r ' 2 6 R t~i R

wherein:
Rl R2 R3 R4 R5 and R6 are each independently selected from:
(1) hydrogen;
~ (2) alkyl having 1 to 6 carbon atoms;
: (3) alkenyl having 2 to 6 carbon atoms;
( CH2 ) nM
wherein n is O to 3 and M is : 25 a) oRl3;
b) halogen;
c) CF3;
:
d) sR13;
~e) phenyl or substituted phenyl ~: wherein substituted phenyl is : : ~ as de~ined below in the ~ : ~ definition of k 3;
, ~ ~

~' ' , 9~;i7~

f) CooR14;

g) - C-RlS;

O
h) -NH-C-R16 wherein R16 is Cl to C6 alkyl, benzyl or phenyl;
i) NR14R14;
i) -N~SO2Rl wherein R
is Cl to C6 alkyl, 4-methylphenyl, phenyl, or CF3;
o k) -C-CH2OH;

1) -SOR 3;
m) coNR14R14;
n) -S2NR R
; 20 ) -So2Rl3;
p) N02;
1i 15 q) -O-C-R

o S) -O- I-OR16;
t) N3;
u) CN;
R7 is H or alkyl of 1 to 6 carbons;
R8 is H or alkyl of 1 to 6 carbon atoms;

.
:

~12~;i77 each R is independently H, OH, Cl to C4-O-alkyl or alkyl of 1 to 4 carbons;
R ~ is COOH; CH2OH; CHO;
NHSO2Rll wherein Rll is O~, alkyl or alkoxy of 1 to 6 carbons, perhaloalkyl of 1 to 6 carbons, phenyl or phenyl substituted by alkyl or alkoxy groups of 1 to 3 carbons, halogen, hydroxy, COOH, CN, formyl or acyl to 1 to 6 carbons; CONHSO2R11; hydroxy-methylketone; CN; or CON(R932;
r is 1 to 6;
each R13 independently is H; Cl to C6 alkyl; benzyl; phenyl or substituted phenyl wherein the substituents are Cl to C3 alkyl, halogen, CN, CF3, CooR14, CH2CooR14, Cl to C3 alkoxy, 15 or Cl to C4 perfluoroalkyl;
each X is independently H, phenyl, benzyl or Cl to C6 alkyl; and, each R15 independently is H, (CH2)mCooR14 wherein m is 0 to 4, Cl to C6 20 alkyl, CF3, phenyl, or substituted phenyl wherein substituted phenyl is as defined above in the definition of R 3;
or a pharmaceutically acceptable salt thereof.
As used herein, the terms "each 25 independently" or the equivalents thereof are employed to describe a number of possible position isomers and~or structural variations. For example, as ; described above, the following unit is attached to position 1 of the tetrahydrocarbazole ring:

(C)r R

The letter r represents possible alkane chains of from 1 to 6 carbon atoms, each having the R and R substituent groups. On each carbon atom of the alkane chain, the R7 and/or R9 S substituent may be different. The above description therefore contemplates structures such as the following for the segment -(CR R9)r~:

(- C - C - C) , (- CH2 -) OH H H

H H H H H H H H
~--C -- C -- C--) ,(--C -- C C -- C -- C--) H H H

(-C - C - C-) , and the like.

If an R9 is OH and R is CO2H, such compounds may form a lactone, and such lactones are to be regarded as part of the present invention.
The alkyl groups referred to above may be 25 straight chain or branched or may include cycloalkyl groups. As used herein, the term "lower" as applied to alkyl, acyl, alkoxy and the like, unless stated otherwise refers to groups having 1 to 6 carbon atoms. Halogen or halo means fluoro, chloro, bromo 30 and/or iodo.
Pharmaceutically acceptable salts of the compounds described herein are included within the ~:

~Z9~57~7 5012P~5011A - 8 - 17323IA

scope of the present invention. Such salts may be prepared from pharmaceutically acceptable non-toxic bases including inorganic bases and organic bases.
Salts derived from inorganic bases include sodium, potassium, lithium, ammonium, calcium, magnesium, ferrous, zinc, copper, manganous, aluminum, ferric, manganic salts and the like. Particularly preferred are the potassium, sodium calcium and magnesium salts.
Salts derived from pharmaceutically acceptable organic 10 non-toxic bases include salts of primary, secondary, and tertiary amines, substituted amines including naturally occurring substituted amines, cyclic amines and ~asic ion exchange resins, such as isopropylamine, tri-methylarnine, diethanolamine, diethylamine, 15 triethylamine, tripropylamine, ethanolamine, 2-dimethylaminoethanol, 2-diethylamino-ethanol, tomethamine, lysine, arginine, histidine, caffeine, procaine, hydrabamine, choline, imidazole, betaine, ethylenediamine, glucosamine, methylglucamine, 20 theobromine, purines piperazine, N,N-dibenzyl-ethylenediamine, piperidine, N-ethyl-piperidine, morpholine, N-ethylmorpholine, polyamine resins and the like.
Preferred compounds of the present invention 25 comprise the compounds of formula I
wherein:
Rl R2 R3 R4 R5 and R6 are each independently selected from:
(1) hydrogen;
(2) alkyl having 1 to 6 carbon atoms;

(3) alkenyl having 2 to 6 carbon atoms;

(4) -(CH2)nM

~a299S77 wherein n i5 0 or 1 and M is as defined previously for Formula I;
R10 is COOH; CH2OH; CHO;
CONHSO2Rll wherein Rll is OH, alkyl or alkoxy of 1 to 6 carbons, perhaloalkyl of 1 to 6 carbons, phenyl or phenyl substituted by alkyl Ol- alkoxy groups of 1 to 3 carbons, halogen, hydroxy, COOH, CN, formyl or acyl to 1 to 6 carbons; hydroxymethylketone;
CN; or CON(R9)2;
r is 1 to 6; and the remaining substituents are as defined previously for Formula I.
More preferred compounds of the present invention comprise the compounds of Formula I.
wherein:
Rl R2 R3 R4, R5 and R6 are each independently selected from:
(1) hydrogen;
(2) alkyl having 1 to 6 carbon atoms;
(3) alkenyl having 2 to 6 carbon atoms;
t4) M wherein M is as defined initially for Formula I;
R10 is COOH; CH2OH; CHO;
hydroxymethylketone;
r is 1 or 2; and the remaining substituents 25 are as defined initially for Formula 1.
Most preferred compounds of the present invention comprise the compounds of Formula I.
wherein:
Rl, R2, R3, R4, R5 and R are 30 each independently selected from:
~1) hydrogen;
(2) alkyl having 1 to 6 carbon atoms;
.

~z~1S~7 5012P/SOllA - 10 - 17323IA

~3) M wherein M is a~ oR13;
b) ha logen;
c) CF~;
d) SR 3;

e) CooR14;
o f) -C-R15;

g) -SoR13;
h) -CONR R
i) _so2NRl4Rl4;
o k) -o-C-R15;
1) CN;
m) N3;
each R9 is independently H, or alkyl of 1 to 4 carbons;
R is COOH;
r is 1 and the remaining substituents are as defined initially for Formula I.
In the above most preferred embodiment, those compounds are particularly preferred wherein at 25 least one of R to R4 is not hydrogen; one of R5 or R6 is not hydrogen; R7 is hydrogen; R9 is hydrogen, and the remaining substituents are as defined in the most preferred embodiment.
A further embodiment of the present 30 invention are the following novel compounds (Table 1).
Among the resolved isomers in Table 1, the (-) isomers, compounds 3,37,39 and 41, are preferred.

:~, ~zs~

501 2P/SO 1 lA - 11 - 1 73231A

Novel Tetrahydrocarbazole A1 kanoi c Aci ds 1~ R2 ~

6' \ 2' \R9 R5~R6 4' Compou~d Rl R2 R5 R6 R, R R R8 -2Q 1 ( Ex . l ~ 6-F H 4 ' -Cl HH, H H H

2 (Ex. 4) 6-OMe H 4'-Cl H H, H H H

3 (Ex. 7) 6-F H 4'-Cl H H, H H H
(-) isomer 4 ~(Ex. 8) 6-F H 4'-C1 H H, H H H
(+) isomer 30 5 I Ex . 9) . 6-F H H H H, H H H

6 (Ex. 10) 6-F H 4'-OMe H H, H H H

.

~Z9~7 5012P/SOllA - 12 - 17323IA

Compound Rl R2 R5 R6 R , R R R8 7 (Ex. 11) 6-F H 3'-Cl 4'-Cl H, H H H

S 8 (Ex. 12) 6-F H H H H, H H Me 9 (Ex 13) H H 4'-Cl H H, H H H

1û (Ex. 14) 6-Cl H 4'-Cl H H, H H H
11 (Ex. 15) 8-Me H 4'-Cl H H, H H H

12 (Ex. 16) 6-Br H 4'-C1 H H, H H H

15 13 (Ex. 17) 6-Me H 4'-Cl H H, H H H

14 (Ex. l9) 8-F H 4'-Cl H H, H H H
:
15 (Ex. 20) 6-F H 4'-Cl H H, H 3-t-Bu H
_ 16 (Ex. 21) 5-F H 4'-Cl H H, H H H

17 (Ex. 21) 7-F H 4'-Cl H H, H H H

25 18 (Ex. 22) 5-Cl 7-Cl 4'-Cl H H, H H H

19 (Ex. 23) 6-Cl 8-Cl 4'-Cl H H, H H H

20 (Ex. 18) 6-F H 4'-Cl H Me, H H H
3û
21 6-F H 4'-Cl H Me, Me H H

22 6-F H 4'-Cl H H, H l-Me H

~2~S~7 5012P/SOllA - 13 - 17323IA

Compound Rl R2 R5 R6 R ,R R R8 23 8-Br H 4'-Cl H H,H H H

S 24 (Ex. 24) 6-CH(Me)2 H 4'-C1 H H,H H H

25 (Ex. 25) 6-C(Me)3 H 4'-C1 H H,H H H

26 (Ex. 26) 6-CF3 H 4'-C1 H H,H H H
27 (Ex. 27) 6-SMe H 4'-C1 H H,H H H

28 (Ex. 28) 6-S(O)Me H 4'-C1 H H,H H H

29 (Ex. 29) 6-5(0)2Me H 4'-Cl H H,H H H

30 (Ex. 30) 8-CH(Me)2 H 4'-C1 H H,H H H

31 (Ex. 31) 8-SMe H 4'-C1 H H,H H H
32 (Ex. 32) 8-S(O)Me H 4'-C1 H H,H H H

33 (Ex. 33) 6-F H 4'-C1 H H,H 3-Me H
__ 34 (Ex. 34) 6-F 8~F 4'-Cl H H,H H H

35 (Ex. 35) 6-Me 8-Me 4'-Cl H H,H H H

36 (Ex. 36) 6-OMe 8-Me 4'-Cl H H,H H H
37 (Ex. 37) 6-F(-)Isomer 8-F 4'-C1 H H,H H H

~29~57q 501ZP/SOllA - 14 - 17323IA

Compound Rl R R5 R6 R ,R R R~3 38 (Ex. 38) 6-F(+)Isomer 8-F 4'-C1 H H,H H H

S 39 (Ex. 39) 8-Me(-)Isomer H 4'-Cl H H,H H H

40 (Ex. 40) 8-Me(+)Isomer H 4'-C1 H H,H H H
.
41 (Ex. 41) 8-F(-)Isomer H 4'-Cl H H,H H H

42 (Ex. 4Z) 8-F(+)Isomer H 4'-C1 H H,H H H
__ 43 6-F 8-F 3'-C1 4'-C1 H,H H H

15 44 6-F 8-F 2'-C1 4'-Cl H,H H H

45 6-F 8-F 4'-OMe H H,H H H
-46 6-F 8-F 4'-OH H H,H H H
47 6-F 8-F 4'-SMe H H,H H H

48 6-F H 4'-S(O)Me H H,H H H

49 6-F 8-F 4'-NHCOMe H H,H H H
-6-F H 4'-S(0)2Me H H,H H H

51 6-F H 4'-F H H,H H H
52 6-F H 4'-Br H H,H H H
_ ~2~5~

Compound Rl R2 R R6 R ,R R R8 53 6-F 8-Me 4'-Cl H H,H H H
-S 54 6-F H 4'-CO2H H H,H H H

6-F H 4'-CO2Me H H,H H H

56 6-F 8~F 4'-n-C3H7 H H,H H H
57 6-F 8-F 3'-I 4'-OH H,H H H

58 6-F 8-F 4'-I H H,H H H

59 6-N3 H ` 4'-Cl H H,H H H
_ 6û 6-F H 4'~N3 H H,H H H

ZS

~%~9~;77 The following reaction schemes illustrate the preparation of the compounds of the present invention:

5 Scheme I Preparation of Formula I Compounds R2 ~ ~ R lower R3 ~ 0 ~ + 0 ~ J alkanoI
10R4 ~ / N-NH2 ~ reflux ¦ ~ HC1 ~R ( C ) r-C02R -I I

Z OR~ R7 ~1 ¦ R R hYdrolysis ~

~)\R8 R9 /~\ (C) r-C02H
2 5R ~ ~6 R5~0~;~ R6 IIIa I ;~

The reaction can be conveniently carried out in an alcohol solvent such as t-butanol, i-butanol, and the like. The hydrolysis of the ester inter-mediates IIIa is conveniently carried out by using 129~57'7 NaOH or KOH ln aqueous ethanol or methanol followed by acidification to obtain compounds of Formula I.
The following ketones ~1,2,4) of structure III are known in the art, and ketone 3 is readily prepared by procedures analogous to those for the known ketones.

ketones of Formula III
No.Structure Reference Ethyl 2-cyclohexanone 1. ~ acetate; commercially 15 // ~ J available (Aldrich) 0 1' C2Et Methyl 2-cyclohexanone 2. ~ propionate; J.A.C.S. 85 ~ ) 207 (1963) O y G. Stork, A. Brizzolara, ~ H. Landesman, ~ J. Scmuszkovicz and CO2ME R. Terrell 3. \ ~ Methyl 4-t-butyl-2-~ cyclohexanone acetate ~ \

y ~CO2 3t'~31~7~7 TABLE 2 (Cont'd) 4. Methyl 2-(2-cyclohexanone) ~ propionate ~ ~ J.A.C.S. 85 207 ~1963) G. Stork, A. Brizzolara, ~ H. Landesman, / CO2ME J. Scmuszkovicz and R. Terrell 5. Ethyl 4-methyl-2-cyclo-~ hexanone acetate O y C2Et The sequence described above is an applic-ation of the Fischer Indole Synthesis. Numerous-indole syntheses are described in reviews, such as,for example "Heterocyclic Compounds" Volume 25, Parts I, II, rII, W. J. Houlihan (Ed.~, Interscience, J.
Wiley & Sons, N. Y., 1979. Appropriate manipulations of functional groups using sequences described in such reviews will lead to the compounds of the present invention.

~2~gs7~

Scheme II Preparation of Hydrazine Derivatives (II~

R3 ~ ~ Et3 N
1, 1 ~ ~ toluene R4 ~ \g-NH2 + R5 ~ R6 reflux 10HCl (IV) (V~

Rl R2 1 Z is a leaving group such as 15 R3 ~ ~ Cl, Br, I, mesylate or tosylate R4 ~ \N-~H2 /\~\R8 R5 ~ R6 II

With regard to Scheme II, the preparation of hydrazine starting materials is illustrated by the preparation of 1-(4-chlorobenzyl)-1-t4-methoxyphenyl~-hydrazine. A mixture of 10 g of p-methoxyphenyl-hydrazine hydrochlorid~, 75 ml of toluene and 11.5 ml of triethylamine was heated at reflux for 60 minutes.
Then, 7.1 g of p chlorobenzyl chloride was added.
After stirring 16 hours at reflux~ triethylamine hydrochloride was filtered off and washed with ethyl 9S"~q ether. The filtrate and washing were concentrated in vacuo and chromatographed on a silica gel column (hexane-ethyl acetate, 9:1) to give 6.64 g of 1-(4-chlorobenzyl)-1-(4-methoxyphenyl)hydrazine. Other ~-- 5 hydrazines, similarly prepared, are also shown in Table 3, below.

H~drazines 4 ~ ~
X~ ) Y~.

Compound No. X Y R Compound Name 1. 4-F 4-Cl H 1-(4-chlorobenzyl)-1-t4-fluorophenyl) hydrazine hydrochloride : 30 2. 3,5-C12 4-Cl H 1-(4-chlorobenzyl)-1-(3,5-dichlorophenyl)hydrazine hydrochloride ~9~577 5012Pt5011A - 21 - 17323IA

_BLE 3 (Cont'd) 3. 4-OMe 4-Cl ~ 1-(4-chlorobenzyl)-1-(4-metho~yphenyl) hydrazine -- 5 hydrochloride 4. 2-Me 4-Cl H 1-(4-chlorobenzyl)-1-t2-~ethylphenyl) hydrazine hydrochloride 5. 4-Me 4-Cl H 1-(4-chlorobenzylj-1-(4-methylphenyl) hydrazine hydrochloride 6. 4-Cl 4-Cl H 1-~4-chlorobenzyl)-1-(4-chlorophenyl) hydrazine hydrochloride : 7. H 4-Cl H 1-(4-chlorobenzyl)-1-(phenyl) - hydrazine hydrochloride 8. 4-Br 4-Cl H 1-(4-chlorQbenz~fl)-1-(4-:~ bromophenyl) hydrazine hydrochlQride 9. 3-F 4-Cl H 1-(4-chlorobenzyl)-l-(3-fluorophenyl) hydrazine hydrochloride : ~ 30 10. 2,4-C12:4-Cl H 1-(4-chlorQbenzyl)-1-(2,4-dichlorophenyl)hydrazine : hydrochloride ~ ~ ' :

~;~9~57~7 TABLE 3 (Cont'd) 11. 4-F H H l-(benzyl)-1-(4-f}uorophenyl) hydrazine hydrochloride 12. 4-F 4-OMe H 1-(4-methoxybenzyl)-1-(4-fluorophenyl~ hydrazine hydrochloride 13. 4-F 3,4-Cl2 H 1-(3,4-dichlorobenzyl)-l-(4-fluoro-phenyl) hydrazine hydrochloride.

14. 4-F H CH3 1~ phenyl)ethyl]-1-(4-fluorophenyl) hydrazine hydrochloride 15. 2-F 4-Cl ~ 4-chlorobenzyl)-1-(2-fluorophenyl) hydrazine hydrochloride.

16. l-[2-(4-chlorophenyl)ethyl]-l-(4-fluorophenyl) hydrazine hydrochloride.

17. 1-(2-propyl)-1-(4-fluorophenyl)hydrazine hydrochloride.

18. 4-CH(Me~2 4-Cl H 1-(4-chlorobenzyl)-1-~4-iso-propylphenyl)hydrazine hydrochloride 19. 4-C(Me)3 4-Cl H 1-(4-chlorobenzyl)-1-~4-tert-butylphenyl)hydrazine)hydro-chloride ;

lZ9~;77 TABLE 3 (Cont'd) 20. 4-CF3 4-Cl H 1-(4-chlorobenzyl)-1-~4-trifluoromethylphenyl)-~ydrazine hydrochloride 21. 4-SMe 4-Cl H 1-~4-chlorobenzyl)-1-(4-methylthiophenyl)hydrazine hydrochloride 22. 2-CH~Me)2 4-Cl ~ 1-(4-chlorobenzyl)-1-(2-iso-propylphenyl)hydrazine hydrochloride .

9~77 5012P/501 lA - 24 - 17323IA

~kemQ~I Alterllative Prepartion of Formula I Comvounds lower alkanol R--~jR7 III ~ IV reflux R3--~ I R~JR 16 H ~ C ) -CO R
R

lEster i ntermedi ate Hydrol ys; s ~A 1) V ~ base ~R

r 2 R9 RS--~R6 Aci d i ntermedi ate Scheme III illustrates an alternative synthesis of the compounds of Formula I. In this Scheme a Fischsr indole synthesis is carried out using a phenylhydrazine IV and the ketone III, followed by hydrolysis. The acid intermediate is 30 then N~benzylated with the reagent V, preferably using a strong base such as potassium t-butoxide to effect the reaction. Acidification of the reaction mixture then yields the free acid of I.

~ 9577 5012P/SOllA - 25 - 17323IA

~beme~ ere~ara~ion of Sulfo)~ides and Sulfones ~f f~a ~ Cnmpounds "` R = TC below.
R _ R is replaced by SR , 5(0)R or ~\N/R~R7 R -R~sR13 or s~o)Rl3 (C)~-~ R9 10 R ~ R6 VI
1.5 eg. of mCPBA
~ S(0)2-R
S(O)R 2 eg. mCPBA ~ T~ C02R

VIIa VIIIa ¦Hyd rol ys i s 1 Hyd rol ys i s S(O)R S(0)2R
TC - C02H ~ TC - C02H

VII VIII

In Scheme IY is illustrated a method of preparing derivatives of Formula I in which one of `~ the substituents among Rl-R4 is a sulfoxide or a sulfone. It will be obvious to one skilled in the 5 art that a sulfoxide or sulfone derivative of R5 or R6 could be prepared in the same way.
Ester VI (a representative of III a, Scheme I) is prepared according to Scheme I or ~cheme III
followed by esterification of acid I. Treatment of VI with a limited amount of an oxidizing agent such as m-chloro-perbenzoic acid yields the sulfoxide ester VIIa, which upon hydrolysis yields sulfoxide acid VII. Further treatment of VIIa with the - oxidizing agent, or treatment of VI with an excess (>2 eq.) of the oxidizing agent yields the sulfone ester VIIIa, hydrolysis of which yields the sulfone acid VIII. Both VII and VIII are representatives of Formula I compounds.

20 ~ch~me V Preparation of Furth~r sDmDallnds Df Formula I

~; = TIIC ~elow /~\R8 R ~ R6 ~Z9~57~

5012P/SOl lA - 27 - 17323IA

LiAlH4 PCC
~ ~1C - C~2R ~ THC - CH20H ~ THC - CHO
(IIIa, Sch~me I) IX X

Hydrol ysi s ' R 52NH2 SOCl 2 Et3N
THC - C02H > THC - COCl - ~ THC - CONHS02R
XI XIV
CH2~/ ~NH

XII XV

15¦ ~12S04 ~ ~, R =R =H
lH2o XIII XVI XVIII
J,R S02Cl lNaN3 N-NH
TH~ ~ CH2NHS2R THC~
N=N
XVII XIX

Other c~mpounds of Formula I can be prepared as indicated in Scheme V. Thus the ester derivative IIIa can be reduced to the alcohol IX by lithium 30 aluminum hydride or other suitabl~ reducing agents.
Alcohol IX can then be oxidized to aldehyde X by pyridinium chlorochromate or other suitable oxidizing agents. Carboxylic acids of Formula I can be converted to the acid chloride XI (the acid bromide ~Z9~S77 or a mi~ed carbonate anhydride could a~lso be used~
which when reacted with diazomethane yields the diazoketone XII. Compound XII, upon reaction with aqueous acid, preferably a nonnucleopllilic acid such S as sulfuric acid or p-toluenesulfonic acid, is converted to the hydro~ymethyl ketone Acid chloride ~I, upon react;on with sulfonamide, RllS92NH2, in the presence of a weak base yields the acyl-sulfonamide XIV. Reaction of XI with an amine, R9R9NH, yields amide XV.
Amide XV can be sequentially reduced, to amine XVI, with diborane or lithium aluminum hydride, and sulfonylated with RllSO2Cl to produce sulfon-amide XVII. Amide XV (when both R9 substituents lS are hydrogen) can be dehydrated by standard reagents to nitrile XVIII, which is converted to tetrazole XIX
by reaction with sodium azide, tri-n-butyltin azide or other suitable methods. Compounds IX, X, XIII, XIV, XV, XVII, XVIII and XIX are representatives of Formula I compounds.
5~hem~YI Preparation ~,r Hvdrazine Derivatives IV

R ~ ) N~N3z ~

R NH2 ~ HCl R NHNH2 HCl IV

~ s~.,!

gLZ95~i77 With regard to 5cheme VI, the preparation of hydrazine starting materials is illustrated by pre--- paration of 4-methylthiophenyl hydrazine hydrochlor-ide. 4-Methylthioaniline (13.9 9) was added dropwise to cold HCl (6N) (50 mL~ and stirred for 5 min in an ice bath. A solution of NaNO2 in water (7.25 9, 15 mL) was then added dropwise and stirred for 15 min.
The cold diazonium salt was then cannulated }nto a stirred cold solution of Na2S2O4 in water ~50 g, 250 mL). After 20 min, ether ~200 mL) was added and the reaction rnixture basified with NaOH(10N).
The ether layer was decanted, washed with brine, dried over Na2SO4 and HCl gas was passed through the ether solution to ~orm the hydrochloride salt which precipitated out. After filtration, there was obtained 7.0 g of pure final product. Other hydrazines, similarly pr~pared, are also shown in Table 3a, below.

TABLE 3a HYDRAZINES

~5 ~3 NHNH2~HC

IV
No. _1 R2 ComPound Name 1 4-SMe H 4-methylthiophenyl hydrazine hydrochloride 9~7 TABLE 4 (Cont'd) .~ 2 2-CH(Me)2 H 2-isopropylphenyl hydrazine hydrochloride 3 2-SMe H 2-methy:Lthiophenyl hydra~ine hydroch:Loride 4 2-Me 4-Me 2,4-dimethylphenyl hydrazine hydrochloride 2-Me 4-OMe 4-methoxy-2-methylphenyl hydrazine hydrochloride In those instances when asymmetric centers are present, more than one stereoisomer is possible, and all possible isomeric forms are deemed to be included within the planar structural representations shown. Optically active (R) and (S) isomers may be resolved using conventional techniques known to the skilled artîsan.
The prostaglandin antagonist properties of the compounds of the present invention can be demonstrated by a number of biological assays, two of which, inhib~tion of platelet aggregation and measurement of PA2 valves are described below.

~z~

Inhibition of Induced Threshold Aggregation of Human Platelets _ _ Human platelet rich plasma (PRP) is prepared from venous blood of male volunteers who have taken no medication for ten days prior to test. Blood is transferred into plastic centrifuge tubes containing 3.8% Trisodium Citrate in 0.9% NaCl (in a ratio of blood to anticoagulant of 9:1), mixed by gentle inversion, and centrifuged at room temperature for ten minutes at 116 g. The supernatant (PRP) is transferred into plastic tubes. Platelet poor plasma (PPP) is obtained by centrifuging the residual blood cells at 4000 y for ten minutes. PRP is left to stand at least one half hour prior to testing.
Platelet Aggregation is measured using a Payton Aggregometer and Recorder. Following calibration of the instrument, a cuvette containing PRP (225 microliters) is incubated for three minutes at 37C. Drug vehicle (control), or a drug concentra-tion is then added in a volume of 0.5 microliter.
After one minute, the aggregating agent (U44069, 9,11-dideoxy-9a,11a-epoxymethano PGF2a) is added to the cuvette in a volume of 25 microliters.
Recording is continued until the maximal response is obtained.
The threshold (approximately 20 - 30% of maximum) aggregation concentration of the agonist to be used is first determined in the presence of the drug vehicle (control). Test compounds are then assayed at 10 or 30 micrograms/ml initially, and if active, are further tested in order to determine the concentration range at which 20-80% of the threshold ~9~77 aggregatory response is inhibited. All drugs are dissolved in dimethylsulfoxide.
The height of the aggregation response (measured in divisions of the recorder paper, 1 ~~ 5 division = 2.5 mm) in the presence of the drug is recorded, and calculated as percent inhibition of the mean height of the control threshold responses. The - IC50 (drug concentration which inhibits 50% of the aggregatory response) is obtained by regression analysis.

Estimation of PA2 Values in Guinea Pig Tracheal _ain - Male albino Hartley strain guinea pigs (300-350 gm) were sacrificed by a blow to the head and exsanguinated. The trachea was removed, freed of extraneous tissue and sectioned into rings of 1-2 mm thickness. Five rings were tied together in series, ensuring that the tracheal muscle lay in the same vertical plane, and the cartilage of each ring then separated at a point directly opposite the muscle.
The chains were suspended under 1 gm resting tension in modified Rrebs solution (~aCl, 6.87; NaHCO3, 2.1; dextrose, 2.1; KCl, 0.32; CaC12, 0.28;
MgSO4, 7H2O, 0.11; KH2PO4, 0.16; gm/L:
equilibrated with 5% CO2 in 2 for 1 hour) containing i~domethacin (1.4 x 10- M) to suppress endogenous protaglandin synthesis, Organ bath temperature was maintained at 37C and 5% CO2 in 2 diffused continously. Isometric tension changes were recorded from a Gould Statham ~UTC 2) force displacement transducer connected to a Beckman Type R

:~l29957~7 Dynograph. For assay purposes initial maximal contractions were elicited with a high concentration `~ of the contractile agonist [U-44069, 9.11~dideoxy-9a,11a-epoxymethano PGF2a] and the tissue sub-sequently washed at intervals until tension returned to baseline. Agonist dose response curves were obtained using a cumulative-dose schedule (4-8 doses) and the preparations then washed at regular intervals until baseline tension was recorded. After an appropriate interval (1-1.5 hrs) agonist dose response curves were repeated in the presence of antagonist drug concentrations. Drug doses were delivered in 10 ~1 volumes 5 minutes prior to the second agonist challenge, and cumulative agonist volumes did not exceed 100 ~1 per bath. EC50 values were obtained by regression analysis and used to calculate 'apparent' and Schild Plot PA2 values by the method of Tallarida and Murray 1981.
Compounds of Formula I can be tested using the following assay to determine their mammalian leukotriene biosynthesis inhibiting activity.

Rat Peritoneal Polymorphonuclear (PMN~
Leukocyte Assay Rats under ether anesthesia are injected (i.p.) with 8 ml of a suspension of sodium caseinate ~6 grams in ca. 50 ml water~. After 15 24 hr. the rats are sacrificed (CO2) and the cells from the peritoneal cavity are recovered by lavage with 20 ml of buffer (Eagles MEM containing 30 mM HEPES adjusted to pH 7.4 with NaOH). The cells are pelleted (350 x g, 5 min.), resuspended in buffer with vigorous ~Z9~ i;77 shaking, filtered, through lens paper, recentrifuged and finally suspended in buffer at a concentration of 10 cells~ml. A 500 ~1 aliquot of PWN suspension and test compound are preincubated for 2 minutes at 37C, followed by the addition of 10 ~M A-23187.
The suspension is stirred for an additional 4 minutes then bioassayed for LTB4 content by adding an aliquot to a second 500 ~1 portion of the PMN at 37C. The LTB4 produced in the first incubation causes aggregation of the second PMN, which is measured as a change in light transmission. The size of the assay aliquot is chosen to give a submaximal transmission change (usually -70%) for the untreated control. The percentage inhibition o~ I,TB~
formation is calculated from the ratio of transmission change in the sample to the transmission change in the compound-free control~
The cytoprotective activity of a compound may be observed in both animals and man by noting the increased resistance of the gastrointestinal mucosa to the no~ious effects of strong irritants, for example, the ulcerogenic effects of aspirin or indomethacin. In addition to lessening the effect of non-steroidal anti-inflammatory drugs on the gastro-intestinal tract, animal studies show that cyto-protective compounds will prevent gastric lesions induced by oFal administration of strong acids, strong bases, ethanol, hypertonic saline solutions and the like.
Two assays can be used to measure cyto-protective ability. These assays are; (A) an ethanol-induced lesion assay and (B) an indomethacin-induced ulcer assay and are described in EP 140,689.

1~99577 In Table 4 below are presented data indicating the prostanoid antagonist activities of compounds of the present invention indicated in Table 1. It is to be noted that PA2 values are on a - 5 logerithmic scale, so that, for instance, a difference between two PA2 values of 1 represents a difference in potency by a factor of 10.
Compounds A, B, C and D in Table 4 are known in the art: U.S. Patent 3,896,145 describes compounds A and C, U.S. Patent 3,868,387 describes compound B, and compound D is descxibed in U.S. Patent 3,905,998.
Compound A, which is a positional isomer of the novel compound 2, is nevertheless almost 12 times less potent as an inhibitor of platelet aggregation and its PA2 is a factor of 14 (antilog of 1.6) less than that of compound 2. The homolog B, with one carbon less than A, shows no significant activity.
Also of interest is the fact that the fully aromatic carbazole analog of the novel compound 2, compound C, does not possess any significant activity. Compounds D and E, isomeric with compound B further demostrate that direct attachment of the carboxyl group to the tetrahydrocarbazole nucleus results in severe loss of activity.
Compound 8 indicates an interesting differentiation of prostaglandin antagonist activity, depending upon the tissue, with very weak action on the guinea pig trachea ~pA2< 6), but with very good potency as an inhibitor of human platelet aggregation (IC50=O.O9~g~ml)-Compound F demonstrates that a hydrogen atomattached to the 9-position (the nitrogen atom) results in severe loss of activity. Likewise, compound G, with a 9-alkyl substituted, has almost completely lost activity. Compound H, with a 2-phenylethyl sub-stituent at the 9-position has also suffered a serious loss of activity by the addition of a CH2 group compared to Compound 1. The last three compounds (F, G and H) demonstrate the requir~ment for a benzyl or substituted benzyl group at position-9 in the compounds of the present invention.
Table 4 Prostanoid Antagonist ~ctivitles 15 Compound Inhibition of PA2 platelet aggregation ~IC50 in ~g/ml) 20 MeO ~

A ~ N ~ CO2H 3.5 6.8 Cl ~Z~95~7 Table 4 (Cont ' d3 ~)\CO;~H >30 < 6 1~

MeO ~ 6 . 5 8 C '~) C02H

20 Cl ~5 `C`
D ~ ~6 Cl/~) 5012P/SOllA - 38 - 17323IA

Table 4 (Cont ' d) Me~ 4.23 6.66 E ~f' C02H

1~ ~
Cl F 1 0 3 . 41 < 6 13,5 <6 2 5 G J~ C02H
Me , Me ~L~9~3S77 5012P/5011A - 39 - . 17323IA

Table 4 ~Cont ' d~

l~o) Q

~ C2~t, 13 . 6 6 . 8:
10 (~
~1 C 03 8 . 6 20 ~/

Cl ~(~ D.30 8.4 3 0 2 ,) C02H

~: . 1 ~
C 1 \~

5012P/SOllA - 40 - 17323IA

Table 4 _(Cont ' d~

~\~) D . 0 5 . 8 . 0 3 J L c02H
10 ~

Cl ~0 0.15 8.7 6 ) L CO~H

25 )~
lleO

~Z~D9S77 5012P/SOllA - 41 - 17323IA

Table 4 (Cont ' d) ~ ~ ; ~ \~ O . 09 3 . 3 7 ) C02H

C~
~ Q o . 09 < 6 ,1~ C02H
9 ~ ISe ,(~ 0.05 ~9 g ) ~ CO H

Cl ~Z~5i77 Table a (Cont ' d) Cl ~ 0.10 ~i.0 ) C02H

,~ O J
Cl '?`: ^` ' olo 9.5 2 5 11 Me ) C02 ~, J
C 1 ~/

~9~

Table 4 ~Cont ' d~

Br - S ~ ~ ~ 0,O9 7.9 - 12 ) CO2H
~

Cl)~

~4) J CO2H 0.08 7.6 20 13~

Cl~J

l 4 F J co2 ~l~J 0.08 9 4 :~LZ9~S77 5012P/SOllA - 44 - 17323IA

Table 4 ~Cont ' d?.

(F) - (F; 3\ N ~) O.17 1~.9 Cl~
ClV~

19 Cl J C02~ 0 . 07 7 . 2 1~
C

~9S~7 Table 4 ~Cont'd) ^`:

~ ; 0 . 31 6 . 8 20 ¦ co~n Me lo 1J

The magnitude of a prophylactic or thera-peutic dose of a compound of Formula I will, of course, vary with the nature or the severity of the condition to be treated and with the particular compound of Formula I and its route of administration.
In general, the daily dose range for anti-asthmatic, anti-allergic, or anti-thrombotic use lies within the range of from about 0.01 mg to about 100 mg per kg body weight of a mammal.
The exact amount of a compound of Formula I
to be used as a cytoprotective agent will depend on, inter alia, whether it is being administered to heal damaged cells or to avoid future damage, on the nature of the damag~d cells (e.g., gastro-intestinal ulcerations vs. nephrotic necrosis), and on the nature of the causative agent. :An e~ample of use of a compound:of Formula I to avoid future damage is co-administration with a non~steroidal anti-inflammatory drug ~for example, indomethacin).

5012P/5~11A - 46 - 17323IA

The effective daily dosage level for compounds of Formula I inducing cytoprotection in ``~ mammals, especially humans, will generally range from about 0.002 mg/kg to about 100 mg/kg, preferably from about 0.02 mg/kg to about 30 mg/kg. The dosage may be administered in single or divided individual doses.
Any suitable route of administration may be employed for providing a mammal, especially a human with an effective dosage of a compound of Formula I.
For example, oral, rectal, topical, parenteral, ocular, nasal, buccal, intravenous and the like may be employed. Dosage forms include tablets, troches, dispersions, suspensions, solutions, capsules, creams, ointments, aerosols and the like.
The pharmaceutical compositions of the present invention comprise a compound of Formula I as an active ingredient or a pharmaceutically acceptable salt thereof, and may also contain a pharmaceutically - acceptable carrier and optionally other therapeutic ~0 ingredients. The term "pharmaceutically acceptable salts" refers to salts prepared from pharmaceutically acceptable non-toxic bases including inorganic ~ases and organic bases. The compositions include compositions suitable for oral, rectal, ophthalmic, pulmonary, nasal, dermal, topical or parenteral ~including subcutaneous, intramuscular and intravenous)/administration, although the most suitable route in any given case will depend on the nature and severity of the conditions being treated and on the nature of the active ingredient. They may be conveniently presented in unit dosage form and prepared by any of the methods well-known in the art of pharmacy.

7~

5~12P/5011A - 47 - 17323IA

For use where a composition for intravenous administration is employed, a suitable dosage range " for anti-asthmatic, or anti-allergic use is from about 0.01 mg to about 20 mg (preferably from about 0.1 mg to about 10 mg) of a compound of Formula I per kg of body weight per day and for cytoprotective use from about 0.002 mg to about 100 mg (preferably from about 0.02 mg to about 30 mg and more preferably from about 0.1 mg to about 10 mg) of a compound of Formula I per kg of body weight per day. In the case where an oral composition is employed, a suitable dosage range for anti-asthmatic, or anti-allergic use is, e.g. from about 1 to about 100 mg of a compound of Formula I per kg of body weight per day, pre~erably from about 5 mg to about 40 mg per kg and for cyto-protective use from about 0.01 mg to about 100 mg (preferably from about 0.1 mg to about 30mg and were preferably from about 0.1 mg to about 10 mg) of a compound of Formula I per kg of body weight per day.
For administration by inhalation, the compounds of the present invention are conveniently delivered in the form of an aerosol spray presenta-tion from pressurized packs or a nebuliser, or a powder which may be formulated as a cartridge from which the powder composition may be inhaled with the aid of a suitable device. The preferred delivery system for inhalation in a metered dose inhalation (~DI) aerosol, which may be formulated as a suspension or solution in ~luorocarbon propellants.
Suitable topical formulations of compound I
include transdermal devices, aerosols, creams, ointments, lotions, dusting powder, and the like.

~LZ995~q In practical use, a compouncl o~ Formula I
can be combirled as the ~ctive ingred;ent in intimate admi~ture with a pharmaceutical carri.er accordiny to conventional pharmaceutical compounding techniques.
The carrier may take a wide variety of forms depending on the form of preparation desired for administration, e.g., oral or parenteral (including intravenous). In preparing the compositions for oral dosage form, any of the usual pharmaceutical media may be employed, such as, for example, water glycols, oils, alcohols, flavoring agents, preservatives, coloring agents and the like in the case of oral liquid preparations, such as, for example, suspensions, elixirs and solutions; or carriers such as starches, sugars, microcrystailine cellulose, diluents, granulating agents, lubricants, binders, disintegrating agents and the like in the case of oral solid preparations such as, for example, powders, capsules and tablets.
Because of their ease of administrat-ion, tablets and capsules represent the most advantageous oral dosage unit form, in which case solid pharmaceutical carriers are obviously employed. If desired, tablets may be sugar coated or enteric coated by standard techniques.
In addition to the common dosage forms set out above, the compounds of Formula I may also be administered by controlled release means and/or delivery devlces such as those described in U.S.
Patent Nos. 3,845,770; 3,91G,899; 3,536,809;
3,598,123; 3,630,200 and 9,008,719.
Pharmaceutical compositions of the present invention suitable for oral administration may be ~;

~Z9~i7~

presented as discrete units such as capsules, cachets or tablets each containing a predetermined amount of ~- the active ingredient, as a powder or granules or as a solution or a suspension in an aqueous liquid, a non-aqueous liquid, an oil-in-water emulsion or a water-in-oil liquid emulsion. Such compositions may be prepared by any of the methods of pharmacy but all methods include the step of bringing into association the active ingredient with the carrier which consti-tutes one or more necessary ingredients. In general,the compositions are prepared by uniformly and intimately admixing the active ingredient with liquid carriers or finely divided solid carriers or both, and then, if necessary, shaping the product into the desired presentation. For example, a tablet may be prepared by compression or molding, optionally with one or more accessory ingredients. Compressed tablets may be prepared by compressing in a suitable machine, the active ingredient in a free-flowing for~ such as powder or granules, optionally mixed with a binder, lubricant, inert diluent, surface active or dispersing agent. Molded tablets may be made by molding in a suitable machine, a mixture of the powdered compound moistened with an inert liquid diluent. Desirably, each tablet contains from about 2.5 mg to about 500 mg of the active ingredient and each cachet or capsule contains fro~ about 2.5 to about 500 mg of the active ingredient.
The following are examples of representative pharmaceutical dosage forms for the compounds of Formula I:

~2~S77 Injectable Suspension ~I.M.) mq/ml Compound of Formula I 2.0 Methylcellulose 5.0 Tween~ 80 0 5 5 Benzyl alcohol 9.0 Benzalkonium chloride 1.0 Water for injection to a total volume of 1 ml Tablet mg~tablet 10 Compound of Formula I 25.0 Microcrystalline Cellulose415.0 Providone 14.0 Pregelatinized Starch 43.5 Magnesium Stearate 2.5 lS

Capsule mq~capsule Compound of Formula I 25.0 Lactose Powder 573.5 20 Magnesium Stearate 1.5 In addition to the compounds of Formula I, the pharmaceutical compositions of the present 2S invention can also contain other active ingredients, such as non-steroidal anti-inflammatory drugs (NSAIDs), pe~ipheral anaIqesic agents such as zomepirac, diflunisal and the like, cyclooxygenase inhibitors, leukotriene antagonists, leukotriene biosynthesis inhibitors, H2 receptor antagonists, antihista~inic agents, prostaglandin antagonists, ACE
inhibitors, and thrombo~ane synthetase inhibitors.

~ILZ9~3577 5012P/SOllA - Sl - 17323IA

The weight ratio of the compound of the Formula I to the second active ingredient may be ~aried and will depend upon the effective dose of each ingredient.
Generally, an effective dose of each will be used.
Thus, for example, when a compound of the Formula I
is combined with a second active ingredient the weight ratio of the compound of ths Formula I to the second ingredient will generally range from about loOO:l to about 1:1000, preferably from 250:1 to 1:200. Combinations of a compound of the Formula I
and other active ingredients will generally be within the aforementioned range, but in each case, an effective dose of each active ingredient should be used.
NSAIDs can be characterized into five groups:
(1) the propionic acid derivatives;
(2) the acetic acid derivatives;
(3) the fenamic acid derivatives;
(9~ the biphenylcarboxylic acid derivatives;
and (5) the oxicams or a pharmaceutically acceptable salt thereof. NSAIDS which are within the scope of this invention are those disclosed ;n EP
140,684.
Pharmaceutical compositions comprising the : Formula I compounds may also contain other inhibitors of the biosynthesis of the leukotrienes such as are disclosed in EP 138,481 tApril 24, 1985), EP 115,394 (August 8, 1984), EP 136,893 (April 10, 1985), and EP
140,709 (May 8, 1985).

5012P/SOllA - 52 - 17323IA

The compounds of the Formula I may also b~
used in combination with leukotriene antagonists such as those disclosed in EP 106,565 (April ~S, 1984) and EP 104,885 ~April 4, 1984), and others known in the art such as those disclosed in European Patent Application ~os. 56,172 and 61,800; and in U.K. Patent Specification No. 2,058,785.

Pharmaceutical compositions comprising the Formula I compounds may also contain as the second active ingredient, other prostaglandin antagonists such as those disclosed in European Patent Application 11,067 ~May 28, 19~0) or other thromboxane antagonists lS such as those disclosed in U.S. 4,237,160. They may also contain histidine decarboxyase inhibitors such as a-fluoromethyl-histidine, described in U.S.
4,325,961. The compounds of the Formula I may also be ad~antageously combined with an Hl or H2-receptor antagonist, such as for instance benadryl, dramamine, histadyl, phenergan, terfenadine, acetamazole, cimetidine, ranitidine, famotidine, aminothiadiazoles disclosed in EP 40,696 (December 2, 1981) and like compounds, such as those disclosed in U.S. Patent Nos. 4,283,403; 4,362,736; 4,394,508.

The pharmaceutical compositions may also contain a K+/Ht ATPase inhibitor such as omeprazole, disclosed in U.S. Pat. g,255,431, and the like. Compounds of I may also be usefully combined with most cell stabilizing agents, such as 1,3-bis~2-carboxychromon-S-yloxy)-2-hydroxypropane and related ~, ''',;

~IZg~5~

compounds described in British Patent Specifications 1,144,905 and 1,144,906. Another useful pharma-ceutical composition comprises the Formula I
compounds in combination with serotonin antagonists such as methysergide, the serotonin antagonists described in ature, Vol. 316, pages 126-131, 1985, and the like.

When the second active ingredient in compositions of this invention is a thromboxane synthetase inhibitor, such inhibitor can be as described in UK 2,038,821 ~e.g., UK 37248 and dazoxiben hydrochloride), U.S.P. 4,217,357 (e.g., UK
34787), U.S.P. 4,444,775 (e.g., CGS 13080), U.S.P.
4,226,878 (e.g., ONO 046), U.S.P. 4,495,357 (e.g., U63557A) U.S.P. 4,273,782 (e.g., UK-3R485), or EP
98,690 (e.g., CV-9151).
An embodiment of the invention is a cardio-vascular composition useful for treating arterialthrombosis which comprises an antithrombotic compound of the Formula I.
A further embodiment of the invention is a cardiovascular composition useful for treating arterial thrombosis which comprises: (1) the antithrombotic Formula I compound defined above; and, (ii) an angiotensin converting enzyme tACE~ inhibitor compound which is a member of the group: carboxyalkyl dipeptide derivatives; captopril [1-(3~mercapto-2-methyl-l-oxopropyl)-L-proline]; 2-[~-(S)-l-ethoxy-carbonyl-3-phenylpropyl)-S-alanyl]-cis,endo-2-azabi-cyclo~3,3,0~octane-3(S)-carboxylic acid; N-((S)-l-~Z~S7~

5012P/5011A - 54 ~ 17323IA

ethoxycarbonyl-3-phenylpropyl~-L-alanyl-N-(2-indanyl)-glycine; l-(N-[(S)-l-ethoxy-carbonyl-3-phenylpropyl]-L-alanyl)-cis,sy~-octahydro-tH-indole-2-S)-carboxylic acid; 2-(N-[(S)-l-ethoxy-carbonyl-3-phenylpropyl~ L-alanyl)-1,2,3,4-tetrahydro-iso-isoquinoline-3(S)-carboxylic acid; and, l-carboxy-methyl-3~S)-(l(S3-ethoxycarbonyl-3-phenylpropylamino)-2,3,4,5-tetrahydro-lH[l]-benzazepine-2-one.
In particular the class of ACE inhibitors which have been found to have a potentiating effect when used in combination with the Formula I compounds are those disclosed in U.S. Patent 4,374,829, which also discloses methods for their preparation.
Of the carboxyalkyl dipeptides disclosed in U.S. Patent 4,374,829, those o particular interest in this invention are N-~l(S)-ethoxycarbonyl-3-phenyl-propyl]-L-alanyl-L-proline, a}so known and referred to herein as enalapril; N-~l(S)-carboxy-3-phenyl-propyl]-L-alanyl-L-proline, also know and referred to herein as enalapril diacid; and, Na-[l(S3-carboxy-3-phenylpropyl]-L-lysyl-L-proline, also known and referred to herein as lisinapril.
The combination composition of the invention can contain varying amounts of (i) the Formula I
antithrombotic compound and (ii) ACE inhibitor antihyperten~ive compounds. The weight ratio of (i):(ii) can range from about 25 to 1; preferably ; from about 10 to 1. In addition to the active ingredients of (i) alone or of (i) and (ii) in combination, the compositions of the invention can also contain other conventional pharmaceutically , ... .

9S~7 acceptable compounding ingredients, as necessary or desired. Such ingredients are generally referred to as carriers or diluents. Conventional procedures for preparing such compositions in appropriate dosage forms can be utilized. Whatever the dosage form, it will contain a pharmaceutically effective amount of the present composition.
The combination compositions can be administered orally or other than orally; e.g., parenterally, by insufflation, topically, rectally, etc.; using appropriate dosage forms; e.g., tablets, capsules, suspensions, solutions, and the like, for oral administration; suspension emulsions, and the like, for parenteral administration; solutions ~or intravenous administration; and ointments, transdermal patches, and the like, for topical adrninistration.
These compositions are formulated similarly to the compositions discussed above.
Treatment dosage for human beings for cardiovascular use can be varied as necessary.
Generally, daily dosages of the composition of the invention can range from about 6000 to about 10 mg;
preferably, from about 3000 to about 20 mg.
The amount of active ingredient that may be combined with the carrier materials to produce a single dosage form for cardiovascular use will vary depending upon the host treated and the particular mode of administration. For example, a formulation intended for oral administration may contain from 5 mg to 5 gm of active agents compounded with an appropriate and convenient amount of carrier material which may vary from about 5 to about 95 percent of the total composition. Dosage unit forms will generally contain between from about 20 mg to about "~ 500 mg of active ingredients.
It will be understood, however, that the ~ 5 specific dose level for any particular patient will depend upon a variety of factoxs including the activity of the specific compound employed, the age, - body weight, general health, sex, diet, time of administration, route of administration, rate of excretion, drug combination and the severity of the particular disease undergoing therapy.
The composition of this invention inhibits platelet accumulation at the damaged endothelial ~ surface via the Formula I compound. This inhibitory effect is potentiated by the presence of the antihypertensive compound.
Thus, the compositions of the invention are useful in treating thrombosis and are also of value in the management of acute and chronic congestive heart failure, and limitation of myocardial infarct damage.
In vivo testing of the composition of this invention in test animals (rabbits) can be used to demonstrate that this composition is pharmaceutically effective in decreasiny platelet-related arterial thrombic formation.
To demonstrate the potentiation of the antihypertensive compound on the anti-thrombotic Formula I compound comprising the combination composition of the invention, the effect of these compounds on test animals (rabbits) can be determined separately and th~n in combination. The effect of a ~299577 different class of antihypertensive agents singly and in combination with the For~ula I compound of the invention can also be determined for co~parative purposes. The methods employed are described in U.S.
Patent 4,558,037 The following examples illustrate the preparation of the compounds of the present invention without, however, limiting the same thereto.
All temperatures are in degrees Celsius.

Reference ComPounds 6-fluoro-1,2,3,4-tetrahydrocarbazol-1-yl-acetic acid Following the procedure of Example 1, but using 1-(4-fluorophenyl) hydrazine hydrochIoride and ethyl-2-cyclohexanone acetate as starting materials, the title compound was prepared.
M.P. 124 -126C

~eference Compounds 9-[1-(2-p-chlorophenyl)ethyl]-6-fluoro-1,2,3,4-tetra-hydrocarbazol-l-yl-acetic acid Following the procedure of Example 2, but using 1-[2-(4-chlorophenyl)ethyl~-1-(4-fluorophenyl) hydrazine hydrochloride and ethyl 2-cyclohexanone acetate as starting materials, the title compound was prepared.
Empirical Formula: C22H2~NFClO2 C H N
Calculated 68.48 5.49 3.63 Found68.15 5.7~ 3.65 ~;',~`1 ,.

~2~ss~

Reference Compounds 6-fluoro-9-isopropyl-1,2,3,4-tetrahydrocarbazol-1-yl-" acetic acid Following the procedure of Example 1, but using 1-~2-propyl)-1- (4-fluorophenyl)hydra2ine hydrochloride and ethyl 2-cyclohexanone acetate as starting materials, the title compound was prepared.
Empirical Formula: C17H20NFO2 M.P. 144-144.5 C
Example 1 9-p-chlorobenzyl-6-fluoro-1,2,3,4-tetrahydrocarbazol-l-yl-acetic acid SteP I
To 3.50 9 of 1-(4-chlorobenzyl~-1-(4-fluoro-phenyl)hydrazine hydrochloride in 70 cc of iso_ propanol was added 2.23 g of ethyl 2-cyclohexanone acetate. The reaction was refluxed under nitrogen for 16 hours. The resulting reaction mixture was then evaporated to dryness and the residue suspended in ether. The solid material was then filtered. The ether filtrate was washed with water, dried and evaporated. The resulting syrup was chromatographed on silica gel to give 2.8 g (42%).
Step II
To ~.59 g of ethyl ester from step I in 10 cc of methanol was added 10 cc of water and 420 mg of potassium hydroxide. The resulting solution was refluxed for 4 hours. Upon cooling the reaction mixture was then acidified with HCl (lN). The resultinq precipitate was filtered and washed with ~;~g~577 water. Analytically pure material was prepared by trituration the solid with a mixture of hexane/ethyl acetate S9:1~ followed by filtration and drying on a high vacuum pump to give 1.24 g of the title compound - 5 (89%)-Analysis calculated for C21HlgNClFO2 C H N Cl F
- Calculated67.83 5.15 3.77 9.53 5.11 Found 67.B85.47 3.63 9.52 5.12 Example 2 3-[9-p-chlorobenzyl-6-fluoro-1,2,3,4-tetrahydro-carbazol-l-yl]-propanoic acid - Following the procedure of Example 1, but using 1-(4-chlorobenzyl)-1-(9-fluorophenyl)hydrazine hydrochloride and methyl-2-cyclohexanone propionate as starting materials, the title compound was prepared.
Empirical formula: C22H21ClFNO2 C H N
Calculated 68.48 5.49 3.63 Found 68.26 5.57 3.60 Example 3 25 3-[9-p-chlorobenzyl-6-methoxy-1,2,3,4-tetrahydro-carbazol-l-yl]-proPanoic acid Following the procedure o~ Example 1, but using l-S4-chlorobenzylj-1-~4-methoxyphenyl)hydrazine hydrochloride and methyl-2-cyclohexanone propionate as starting materials, the title compound was prepared.

5012P/SOllA - 60 - 17323IA

Empirical formula: C~2H22ClN03 C H N Cl Calculated 69.436.0~ 3.52 8.91 Found 69.24 5.98 3.60 8.85 _ 5 Example 4 9-p-chlorobenzyl-6-methoxy-1,2,3,4-tetrahydrocarbazol-l-yl-acetic acid Following the procedure of Example 1, but using 1-(4-chlorobenzyl~-1-(4-methoxyphenyl~hydrazine hydrochloride and ethyl-2-cyclohexanone acetate as starting materials, the title compound was prepared.
M.P. 152-153C.

Example 5 2-[9-p-chlorobenzyl-6-fluoro-1,2,3,4-tetrahydro-carbazol-l-yl]ethanol 1.10 g of 9-p-chlorobenzyl-6-fluoro-1,2,3,4-tetrahydrocarbazo-l-yl-acetic acid ethyl ester was dissolved in 500-ml dry tetrahydrofuran and the reaction was cooled at 0C and one equivalent of lithium aluminium hydride (LAH3 was added portion wise. The reaction was allowed to warm up to room temperature and stirred for 16 hrs. The reaction mixture was quenched with NH4Cl (aq.). Ethyl acetate was added ~100 ml) and the organic phase ~eparated, washed with water and brine, dried and evaporated. The product was isolated by column chromatography.
M.P. 98.0 - 98.5 C.

~2~5~7 Example 6 3-[9-p-chlorobenzyl-6-methoxy-1,2,3,4-tetrahydro-carbazol-l-yl]-propanol Following the procedure of Example 5, but using 3-[9-p-chlorobenzyl-6-methoxy-1,2,3,4-tetra-hydrocarbazo-l-yl~-propanoic acid ethyl ester from Example 3 as starting material, the title compound was prepared.
Empirical formula: C23H26ClNO2 C H N
Calculated 71.95 6.83 3.65 Found 71.86 6.65 3.81 Example 7 15 (-)9-p-chlorobenzyl-6-fluoro-1,2,3,4-tetrahydro-carbazol-l-yl-acetic acid SteP I
10.0 g of 9-p-chlorobenzyl-6-fluoro-1,2,3,4-tetrahydrocarbazo-l-yl-acetic acid from Example 1 was dissolved in a mixture of hot (refluxing) aceto-nitrile (150 cc), and ethanol (25cc) and 4.4 g of d(~) ephedrine wa~s added. The reflux was continued for 15 min. and the hot solution was filtered and allowed to cool to room temperature. Crystals separated from the solution and were separated by filtration. After three recrystallizations from acetonitrile 3.9 g of the pure salt was obtained.

Step II
3.9 g of pure salt from step I was dissolved in 200 cc of methanol and acidified using 1 N hydro-chloric acid. Water was added and the crystals were ~L2~15~

separated by filtration and dryed under vacuum. Upon trituration with hexane ethyl acetate mixture (9:1) "~ the title compound was prepared.
aD = -42.5 (methanol) M.P. 151 - 15105C.
_ 5 Example 8 (+~9-p-chlorobenzyl-6-fluoro-1,2,3,4-tetrahydro-carbazol-l-Yl-acetic acid Following the method of Example 7, but using 10 1(-) ephedrine and 9-p-chlorobenzyl-6-fluoro-1,2,3,4-tetrahydrocarbazo-l-yl-acetic acid as starting material, the title compound was prepared.
aD = ~43.0 (methanol) M.P. 150 - 150.5C.

Example 9 9-benzyl-6-fluoro-1,2,3,4-tetrahydrocarbazol-1-yl-acetic acid Following the procedure of Example 1, but using l-(benzyl)-l- (4-fluorophenyl)hydrazine hydrochloride and ethyl 2-cyclohexanone acetate as starting materials, the title compound was prepared.
Analysis calculated for C21H20NFO2 C H N
Calculated 74.76 5.98 4.15 25 Found 74.95 6.07 3.90 , Example 10 9-p-metho~ybenzyl-6-fluoro-1,2,3,4-tetrahydrocarbazol-l-yl-acetic acid Following the procedure of Example 1, but using 1-(4-methoxybenzyl)-1-~4-fluorophenyl~hydrazine hydrochloride and ethyl 2-cyclohexanone ac0tate as stating materials, the title compound was prepared.

~29~5~7 Analysis calculated for C22H22NFO3 C H N
~`~ Calculated 71.92 6.04 3.91 Found 71.70 6.22 4.05 _. 5 Examp1~_11 9-(3,4-dichloro)benzyl-6-fluoro-1,2,3,4-tetrahydro-carbazol-l-yl-acetic acid Following the proceduré of Example 1, but using 1-(3,4-dichlorobenzyl~- 1-t4-fluorophenyl) hydrazine hydrochloride and ethyl 2-cyclohexanone acetate as starting materials, the title compound was prepared.
Analysis calculated for C21H18NC12FO2.

Calculated 62.08 4.47 3.95 Found 61.96 4.71 3.67 Example 12 9-[1-(1-phenyl)ethyl]-6-fluoro-1,2,3,4-tetrahydro-carbazol-l-yl-acetic acid Following the procedure of Example 1, but using l-[l-(l-phenyl)ethyl]-1-~4-fluorophenyl)-hydrazin~e hydrochloride and ethyl 2-cyclohexanone acetate as starting materials, the title compound was prepared.
C H N F
Calculated 75.196.31 3.99 5.41 Found 75.18 6.054.11 5.51 .

~2995~7 Example 13 ""~ 9-p-chlorobenzyl-1,2,3,4-tetrahydrocarbazol-1-yl-acetic acid Following the procedure of Example 1, but using 1-(4-chlorobenzyl3~1-(phenyl)hydrazine hydro-chloride and ethyl 2-cyclohexanone acetate as starting materials, the title compound was prepared.
Empirical Formula: C21H20NClO2 C H N Cl Calculated 71.28 5.70 3.96 10.02 Found 70.7n 5.90 3.82 10.23 xample 19 9-p-chlorobenzyl-6-chloro-1,2,3,4-tetrahydrocarbazol-l-yl-acet~c acid Following the procedure of Example 1, but using 1-(4-chlorobenzyl)-1-(4-chlorophenyl)hydrazine hydrochloride and ethyl-2-cyclohexanone acetate as starting materials, the title compound was prepared.
Empirical Formula: C21H19NC12O2 C H N Cl Calculated 69.96 4.93 3.61 18.26 Found 65.20 5.16 3.38 18.04 Example 15 9-p-chlorobe~zyl-8-methyl-1,2,3,4-tetrahydrocarbazol-l-yl-acetic acid Following the procedure of Example 1, but using 1-(4-chlorobenzyl~-1-(2-methylphenyl)hydrazine hydrochloride and ethyl-2-cyclohexanone acetate as starting materials,~the title compound was prepared.

~9~577 Empirical Formula: C22H22NC1O2 C H N Cl Calculated 71.83 6.03 3.81 9.64 Found 72.196.23 4.12 9.84 Example 16 6-bromo-9-p-chlorobenzyl-1,2,3,4-tetrahydrocarbazol-1-yl-acetic acid Following the procedure of Example 1, but using 1-~4-chlorobenzyl)-1-(4-bromophenyl)hydrazine hydrochloride and ethyl 2-cyclohexanone acetate as starting materials, the title compound was prepared.
Empirical formula: C21HlgBrClNO2 ~ C H N Br Cl 15 Calculated 58.29 4.43 3.24 18.46 8.19 Found 58.49 4.60 3.44 18.50 8.27 Example 17 9-p-chlorobenzyl-6-methyl-1,2,3,4-tetrahydrocarbazol-l-yl-acetic acid Following the procedure of Example 1, but using 1-(4-chlorobenzyl)-1-(4-methylphenyl)hydrazine hydrochloride and ethyl 2-cyclohexanone acetate as starting materials, the title compound was prepared.
Empirical formula: C22H22ClNO2 C H N Cl Calculated ~1.836.03 3.81 9.64 Found 71.72 6.14 3.77 9.88 ~ ' ~LZ~ 5~C7 5012P~5011A - 66 - 17323IA

E~ample 18 2-[9-p-chlorobenzyl-6-fluoro-1,2,3,4-tetrahydro-`~ carbazol-l-yl-]propanoic acid.
Following the procedure of Example 1, but using 1-~4-chlorobenzyl)-1-(4-1uorophenyl) hydrazine hydrochloride and methyl 2-(2-cyclohexanone) pro-pionate as starting materials, the title compound was prepared.
M.P. 203-205C
Example 19 9-p-chlorobenzyl-8 fluoro-1,2,3,4-tetrahydrocarbazol-l-yl-acetic acid.
Followiny the procedure of Example 1, but using 1-(4-chlorobenzyl)-1-(2-fluorophenyl) hydrozine hydrochloride and ethyl Z-cyclohexanone acetate as starting materials, the title compound was prepared.
M.P. 228-229C.

Example 20 3 [(a and B)-t-butyl-6-fluoro-9-(p-chlorobenzyl)-1,2,3,4-tetrahydrocarbazol-1-yl]acetic acid.
Following the procedure of Example 1 but using 4-t-butyl-2-carbomethoxymethyl cyclohexanone and 1-t4-chlorobenzyl)-4-fluorophenylhydrazine hydro-chloride as the starting materials, the title compound was prepared~
M.P. 210-211C.

,n ~ g ~

5012P~5011A - 67 - 17323IA

Example 21 9-p-chlorobenzyl-5-fluoro-1,2,3,4-tetrahydrocarbazol-`-~ l-yl-acetic acid and 9-p-chlorobenzyl-7-fluoro-1,2,3,4-tetrahydocarbazol-1-yl-acetic acid (mixture).
Following the procedure of Example 1, but using 1-(4-chlorobenzyl)-1-(3-fluorophenyl)hydrazine hydrocloride and ethyl 2-cyclohexanone acetate as starting materials, the title compounds were prepared.
Empirical Formula: C21HlgNClFO2 M.P. 211-219C

Example 22 9-p-chlorobenzyl-5,7-dichloro-1,2,3,4-tetrahydro-carba~ol-l-yl-ac_tic acid.
Following the procedure of Example 1, but using l-(4-chlorobenzyl)-1-(3-5 dichlorophenyl)-hydrozine hydrochloride and ethyl 2-cyclohe2anone acetate as starting materials, the title compound was prepared.
Empirical formula: C21H18NC13O2 M.P. 204-206C

Example 23 9-p-chlorobenzyl-6,8-dichloro-1,2,3,4-tetrahydro-carbazol-l-yl-acetic acid.
Following the procedure of Example 1, but using 1-(4-cnlorobenzyl)-1-(2-4-dichlorophenyl)-hydrozine hydrochloride and ethyl 2-cyclohexanone acetate as starting materials, the title compound was prepared.
Empirical formula: C21H18NC13O2 M.P. 203-204C.

~Z9~57~7 Example 24 9-p-Chlorobenzyl-6-isopropyl-1,2,3,4-tetrahydrocar--~ bazol-l-Yl-acetic acid Following the procedure of Example 1, but using 1(4-chlorobenzyl)-1-(4-isopropylphenyl~-hydrazine hydrochloride and ethyl 2-cyclohexanone acetate as starting material, the title compound was prepared.
Empirical Formula: C24H26ClNO2 C H N Cl Calc: 72.81 6.62 3.5~4 B.95 Found: 72.83 7.09 3.61 9.21 Example 25 9-p-Chlorobenzyl-6-tert-butyl-1,2,3,4-tetrahydrocar-bazol-l-yl-acetic acid Following the procedure of Example 1, but using 1-(4-chlorobenzyl)-1-t4-tert-butylphenyl) hydrazine hydrochloride and ethyl2-cyclohexanone acetate as starting materials, the title compound was prepared.
Empirical Formula: C25H28ClNO2 C H ~ Cl Calc. 73.24 6.88 3.42 8.65 25 Found: 73.21 7.32 3.10 8.26 ~ Example 26 9-p-Chlorobenzyl-6-trifluoromethyl-1,2,3,4-tetra-hydrocarbazol-l-yl-acetic acid _ Following the procedure of Example 1, but using l-(4-chlorobenzyl)-1-(4-trifluoromethylphenyl) hydrazine hydrochloride and ethyl 2~cyclohexanone ~Z~5~7 acetate as starting materials, the title compound was prepared. m.p. 167-168C.
""
_ Example 27 9-p-Chlorobenzyl-6-methylthio-1,2,3,4-tetrahydro-carbazol-l-yl-acetic acid Following the procedure of Example 1, but using l-(4-chlorobenzyl)-1-(4-methylthiophenyl) hydrazine hydrochloride and ethyl 2-cyclohexanone acetate as starting materials, the title compound was prepared.
Empirical Formula: C22~22ClNO2S
C H N S Cl Calc: 66.07 5.54 3.50 8.02 8.86 15 Found: 66.27 5.83 3.38 8.00 8.70 ExamPle 28 9-p-Chlorobenzyl-6-methylsulfinyl-1,2,3,4-tetrahydro-carbazol-l-yl-acetic acid Step I
To 498 mg of ethyl 9-p-chlorobenzyl-6-methyl-thio-1,2,3,4-tetrahydrocarbazol-1-yl-acetate from ~xample 27, Step I, in 10 cc of methylene chloride was added 300 mg of m-chloro perbenzoic acid. The resulting mixture was stirred for 1.5 hours at room temperature. The reaction mixture was diluted with ether and washed consecutively with a solution of sodium bicarbonate, water and brine. The crude product obtained after evaporation of the organic layer was purified on silica gel by flash chromato-graphy eluting with 20% hexane/ethyl acetate and yielded 420 mg (82%) of the pure sulfoxide derivative.

~ 99~77 .~ Step XI
Following the procedure of Example 1, Step -- II, but using the ethyl Pster from Step I, there was obtained the title compound. m.p. 105-107C.
Empirical Formula: C22H22ClNO3S
C H N S Cl Calc: 63.53 5.33 3.37 7.71 8.52 Found: 63.31 5.03 ~.94 7.69 8.48 Example 29 9-p-Chloro~enzyl-6-methylsulfonyl-1,2,3,4-tetrahydro-carbazol-l-Y1-acetic acid steP I
To 439 mg of ethyl 9-p-chlorobenzyl-6-methyl-sulfinyl-1,2,3,4-tetrahydrocarbazol-1-yl-acetate from Example 28, Step I, in 10 cc of methylene chloride was added 3~3 mg of m-chloro perbenzoic acid. The resulting mixture was stirred for 18 hours at room temperature. The reaction mixture was diluted with ether and washed consecutively with a solution of sodium bicarbonate, water and brine. The crude product obtained after evaporation of the organic layer was purified on silica gel by flash chromato-graphy eluting with 30% hexane/ethyl acetate and yielded 200 mg (92%) of the pure sulfone derivative.
.
Step II
Following the procedure of Example 1, Step ~ I, but using the ethyl ester from Step I, there was obtained the title compound. ~.p. 101-102C.

:

lZ~'$7~

ExamPle 30 9-p-Chlorobenzyl-8-isopropyl-1,2,3,4-tetrahydrocar-~- bazol-l-yl-acetic acid Following the procedure of Example 1, but using 1-(4-chlorobenzyl)-1-(2-isopropylphenyl) hydrazine hydrochloride and ethyl 2-cyclohexanone acetate as starting materials, the title compound was ~ prepared.
Empirical Formula: C24H26ClNO2 C H
Calc: 72.81 6.62 Found: 72.59 6.90 Example 31 9-p-Chlorobenzyl-8-methylthio-1,2,3,4-tetrahydrocar-bazol-l-yl-acetic acid Following the procedure of Example 34, but using 1-(2-methylthiophenyl) hydrazine hydrochloride and ethyl 2-cyclohexanone acetate as starting materials, the title compound was prepared. m.p.
141-142C.

Example 32 9~p-Chlorobenzyl-8-methylsulfinyl-1,2,3,4-tetrahydro-carbazol-l-Yl-acetic acid Following the procedure of Example 28, but using the et~yl ester from Esample 31, Step II, there was obtained the title compound. m.p. 119-120.5C.

~L2~'~S77 Example 33 9-p-Chlorobenzyl-6-fluoro-3-methyl-1,2,3,4-tetrahydro-`~- carbazol-l-yl-acetic acid Following the procedure of Example 1, but using 1-(4-chlorobenzyl)-1-(4-fluorophenyl) hydrazine hydrochloride and ethyl 4-methyl-2-cyclohexanone acetate as starting materials, the title compound was prepared. m.p. 205-206C.
Empirical Formula: C22H21FClNO2 C H N Cl Calc: 68.48 5.49 3.63 9.19 Found: 68.80 5.50 3.30 9.47 Example 34 9-p-Chlorobenzyl-6,8-difluoro-1,2,3,4-tetrahydro-carbazol-l-Y1=acetic acid Step I
To 114 g of 1-(2,4-difluorophenyl) hydrazine hydrochloride in 350 cc of 2-propanol containing 40 cc of acetyl chloride was added 138 g of ethyl 2-cyclohexanone acetate. The reaction was refluxed under nitrogen for 2 days. After cooling, 200 cc of ether was added and the precipitate filtered. The filtrate was evaporated to dryness. The resulting residue was dissolved in a (1:1) mixture of ether/ethyl acetate and consecutively washed with water, sodiu~ bicarbonate solution and brine. The organic layer was dried over sodium sulfate and evaporated to dryness. The crude product was passed through a silica gel bed eluting with 5% ethyl acetate/hexane to yield 84 g of a 1:2 mixture of ethyl and isopropyl esters.

~Z~ 77 Step II
84 g of esters from Step I was dissolved in 250 cc of methanol and 400 cc of sodium hydroxide (lN) was added and reflu~ed 4 hours. After cooling, the reaction mixture was washed with a (1 1~ mixture of ether/hexane and the aqueous layer was acidified with HCl (lN). The resulting precipate was filtered, washed with water and air dried to afford 50 g of 6,8-difluoro-1,2,3,4-tetrahydrocarbazol-1-yl~acetic acid.

Step III
A solution of 11:1 g of acid from Step II in 100 cc of THF was added portionwise 10.3 g of potassium tert-butoxide. The resulting mixture was stirred for 45 min. at room temperature and 10.3 g p-chlorobenzyl bromide was added portionwise. The reaction mixture was stirred 18 hours at room - temperature. The resulting mixture was diluted with lOQ cc of water and washed with hexane. The aqueous layer was acidified with HCl (lN) and the resulting precipitate filtered washed with water and air-dried to afford 9.4 g of the title compound. m.p.
168.5-170C.
Example 35 9-p-Chlorobenzyl-6,8-dimethyl-1,2,3,4-tetrahydro-arbazol-l-yl-acetic acid Following the procedure of Example 34, but using 1-(2,4-dimethylphenyl) hydrazine hydrochloride and ethyl 2-cyclohexanone acetate in Step I as starting materials, the title compound was prepared.
m.p. 187-188C.

99~77 Example 36 ~-p-Chlorobenzyl-6-methoxy-8-methyl--1,2,3,4-tetrahydro-`~ carbazol-l-yl-acetic acid Following the procedure of Example 34, but using 1-(4-methoxy-2-methylphenyl) hydrazine hydrochloride and ethyl 2-cyclohexanone acetate as starting materials, the little compound was prepared. m.p. 188-188.5C.

Example 37 (-)-9-p-Chlorobenzyl-6,8-difluoro-1,2,3,4-tetrahydro-carbazol-l-Yl-acetic acid Following the method of Example 7, but using d(~)ephedrine and 9-p-chlorobenzyl-6,8-difluoro-15 1,2,3,4-tetrahydrocarbazol-1-yl-acetic acid from Example 34 as starting materials, the title compound was prepared. from Example 34 [a]D = -6g.3 (methanol) m.p. 130-131C.

Example 38 (+)-9-p-Chlorobenzyl-6,8-difluoro-1,2,3,4-tetrahydro-carbazol-l-yl-acetic_ac;d Following the method oE Example 371 but using l~-)ephedrine, the title compound was prepared.
25 [a]D = +61.5 ~methanol) m.p. 129.5-130C.

Example 39 ~ 9-p-Chlorobenzyl-8-methyl-1,2,3,4-tetrahydro-carbazol-l-Yl-acetic acid Following the method of Example 7, but using d(~)ephedrine and 9-p-chlorobenzyl-8-methyl-1,2,3,4-tetrahydrocarbazol-l-yl-acetic acid from Example 15 lZ~9577 5012P~5011A - 75 - 17323IA

as starting materials, the title compound was prepared. [a]D = -51.6 (methanol) m.p.
196-198C.

_ Example 40 ~ 9-p-Chlorobenzyl-8-methyl-1,2,3,4-tetrahydro-carbazol-l-yl-acetic acid Following the method of Example 39, but using l(-~ephedrine, the title compound was prepared.
10 [a]D = +45.9 (methanol) m.p. 135-197C.

Example 41 (-)-9-p-Chlorobenzyl-8-fluoro-1,2,3,4-tetrahydro-carbazol-l-yl-acetic acid Following the method of Example 7, but using d(~)ephedrine and 9-p-chlorobenzyl-8-fluoro-1,2,3,4-tetrahydrocarbazol-l-yl-acetic acid from Example 19 as starting materials, the title compound was prepared. [a]D = -62.1 (methanol) m.p. 74-75C.
Example 42 ~ 9-p~Chlorobenzyl-8-fluoro-1,2,3,4-tetrahydro-carbazol-l-yl-acetic acid Following the method of Example 41, but 25 using l(-)ephedrine, the title compound was prepared.
[a]D = f65.2 (methanol) m.p. 94-94.5C.

ExamPle- 43 2-(9-p-Chlorobenzyl-6,8-difluoro-1,2,3,4-tetrahydro-carbazol-l-yl)ethanol Following the procedure of Example 5, but using a mixture of 9-p-chlorobenzyl-6,8-difluoro-g577 5012P~5011A - 76 - 17323IA

1,2,3,4-carhazol-1-yl-acetic acid ethyl and isopropyl esters from Example 34 as starting materials, the title compound is obtained.

- 5 Exam~le 44 (-) or t+) 2-(9-p-Chlorobenzyl-6,8-difluoro-1,2,3,4-tetrahYdrocarbazol-l-yl)sthanol Following the procedure of Example 5, but using (-) or (+) 9-p-chlorobenzyl-6,8-difluoro-10 1,2,3,4-tetrahydrocarbazol-1-yl-acetic acid from Example 37 or 38 as starting material, the title compounds are obtained.

Claims (27)

1. A compound of the formula:

I

wherein:
R1, R2, R3, R4, R5 and R6 are each independently selected from:
(1) hydrogen;
(2) alkyl having 1 to 6 carbon atoms;
(3) alkenyl having 2 to 6 carbon atoms;
(4) -(CH2)nM
wherein n is 0 to 3 and M is a) OR13;
b) halogen;
c) CF3;
d) SR13;
e) phenyl or substituted phenyl wherein substituted phenyl is as defined below in the definition of R13;

f) COOR14;
g) -?-R15;
h) -NH-?-R16 wherein R16 is C1 to C6 alkyl, benzyl or phenyl;
i) NR14R14;
j) -NHSO2R17 wherein R17 is C1 to C6 alkyl, 4-methylphenyl, phenyl, or CF3;
k) -?-CH2OH;
l) -SOR13;
CONR14R14;
o) -SO2R13;
p) NO2;
r) -O-?-NR14R14;
s) -O-?-OR16;
t) CN;
u) N3;
R7 is H or alkyl of 1 to 6 carbons;
R8 is H or alkyl of 1 to 6 carbon atoms;
each R9 is independently H, OH, C1 to C4-O-alkyl or alkyl of 1 to 4 carbons;

R10 is COOH; CH2OH; CHO;
NHSO2R11 wherein R11 is OH, alkyl or alkoxy of 1 to 6 carbons, perhaloalkyl of 1 to 6 carbons, phenyl or phenyl substituted by alkyl or alkoxy groups of 1 to 3 carbons, halogen, hydroxy, COOH, CN, formyl or acyl of 1 to 6 carbons; CONHSO2R11;
hydroxymethylketone; CN; or CON(R9)2;
r is 1 to 6;
each R13 independently is H; C1 to C6 alkyl; benzyl, phenyl or substituted phenyl wherein the substituents are C1 to C3 alkyl, halogen, CN, CF3, COOR14, CH2COOR14, C1 to C3 alkoxy, or C1 to C4 perfluoroalkyl;
each R14 is independently H, phenyl, benzyl or C1 to C6 alkyl; and, each R15 independently is H, (CH2)mCOOR14 wherein m is 0 to 4, C1 to C6 alkyl, CF3, phenyl, or substituted phenyl wherein substituted phenyl is as defined above in the definition of R13;
or a pharmaceutically acceptable salt thereof.

2. A compound according to Claim 1, wherein:
R1, R2, R3, R4, R5 and R6 are each independently selected from:
(1) hydrogen;
(2) alkyl having 1 to 6 carbon atoms;
(3) alkenyl having 2 to 6 carbon atoms;
(4) -(CH2)nM
wherein n is 0 or 1 and M is as defined previously for Claim 1;

R10 is COOH; CH2OH; CHO;
CONHSO2R11 wherein R11 is OH, alkyl or alkoxy of 1 to 6 carbons, perhaloalkyl of 1 to 6 carbons, phenyl or phenyl substituted by alkyl or alkoxy groups of 1 to 3 carbons, halogen, hydroxy, COOH, CN, formyl or acyl of 1 to 6 carbons; hydroxymethylketone;
CN; or CON(R9)2;
r is 1 to 6.

3. A compound according to Claim 2, wherein:
R1, R2, R3, R4, R5 and R6 are each independently selected from:
(1) hydrogen;
(2) alkyl having 1 to 6 carbon atoms;
(3) alkenyl having 2 to 6 carbon atoms;
(4) M wherein M is as defined initially for Claim 1;
R10 is COOH; CH2OH; CHO;
hydroxymethylketone;
r is 1 or 2.

4. A compound according to Claim 3, wherein:
R1, R2, R3, R4, R5 and R6 are each independently selected from:
(1) hydrogen;
(2) alkyl having 1 to 6 carbon atoms, (3) M wherein M is a) OR13;
b) halogen;
c) CF3;

d) SR13;
e) COOR14 f) -?-R15;
g) -SOR13;
h) -CONR14R14;
i) -SO2NR14R14;
j) -SO2R13;
k) -O-?-R15;;
l) CN;
m) N3;
each R9 is independently H, or alkyl of 1 to 4 carbons;
R10 is COOH;
r is 1.

5. A compound according to Claim 1 which is:
9-p-chlorobenzyl-6-fluoro-1,2,3,4-tetrahydrocarbazol-1-yl-acetic acid;
3-[9-p-chlorobenzyl-6-fluoro-1,2,3,4-tetrahydro-carbazol-1-yl]-propanoic acid;
3-[9-p-chlorobenzyl-6-methoxy-1,2,3,4-tetrahydro-carbazol-1-yl]-propanoic acid;
9-p-chlorobenzl-6-methoxy-1,2,3,4 tetrahydrocarbazol-1-yl-acetic acid;
2-[9-p-chlorobenzyl-6-fluoro-1,2,3,4-tetrahydro-carbazol-1-yl]-1 ethanol;
3-[9-p-chlorobenzyl-6-methoxy-1,2,3,4-tetrahydro-carbazol-1-yl]-propanol;

(-)9-p-chlorobenzyl-6-fluoro-1,2,3,4-tetrahydro-carbazol-1-yl-acetic acid;
(+)9-p-chlorobenzyl-6-fluoro-1,2,3,4-tetrahydro-carbazol-1-yl-acetic acid;
9-benzyl-6-fluoro-1,2,3,4-tetrahydrocarbazol-1-yl-acetic acid;
9-p-methoxybenzyl-6-fluoro-1,2,3,4-tetrahydrocarbazol-1-yl-acetic acid;
9-(3,4-dichloro)benzyl-6-fluoro-1,2,3,4-tetrahydro-carbazol-1-yl-acetic acid;
9-[1-(1-phenyl)ethyl]-6-fluoro-1,2,3,4-tetrahydro-carbazol-1-yl-acetic acid;
9-p-chlorobenzyl-1,2,3,4-tetrahydrocarbazol-1-yl-acetic acid;
9-p-chlorobenzyl-6-chloro-1,2,3,4-tetrahydrocarbazol-1-yl-acetic acid;
9-p-chlorobenzyl-8-methyl-1,2,3,4-tetrahydrocarbazol-1-yl-acetic acid;

6-bromo-9-p-chlorobenzyl-1,2,3,4-tetrahydrocarbazol-1-yl-acetic acid;
9-p-chlorobenzyl-6-methyl-1,2,3,4-tetrahydrocarbazol-1-yl acetic acid;
2-[9-p-chlorobenzyl-6-fluoro-1,2,3,4-tetrahydrocarbazol -1-yl-]propanoic acid;
9-p-chlorobenzyl-8-fluoro-1,2,3,4-tetrahydrocarbozol-l-yl-acetic acid;
3-[(.alpha. and .beta.)-t-butyl-6-fluoro-9-(p-chlorobenzyl)-1,2,3,4-tetrahydrocarbazol-1-yl]acetic acid;
9-p-chlorobenzyl-5-fluoro-1,2,3,4-tetrahydrocarbazol-1-yl-acetic acid;
9-p-chlorobenzyl-7-fluoro-1,2,3,4-tetrahydocarbazol-1-yl-acetic acid;

9-p-chlorobenzyl-5,7-dichloro-1,2,3,4-tetrahydro-carbazol-1-yl-acetic acid;
9-p-chlorobenzyl-6,8-dichloro-1,2,3,4-tetrahydro-carbazol-1-yl-acetic acid.
9-p-chlorobenzyl-6-isopropyl-1,2,3,4-tetrahydrocar-bazol-1-yl-acetic acid;
9-p-chlorobenzyl-6-tert-butyl-1,2,3,4-tetrahydrocar-bazol-1-yl-acetic acid;
9-p-chlorobenzyl-6-trifluoromethyl-1,2,3,4-tetra-hydrocarbazol-1-yl-acetic acid;
9-p-chlorobenzyl-6-methylthio-1,2,3,4-tetrahydro-carbazol-1-yl-acetic acid;
9-p-chlorobenzyl-6-methylsulfinyl-1,2,3,4-tetrahydro-carbazol-1-yl-acetic acid;
9-p-chlorobenzyl-6-methylsulfonyl-1,2,3,4-tetrahydro-carbazol-1-yl-acetic acid;
9-p-chlorobenzyl-8-isopropyl-1,2,3,4-tetrahydrocar-bazol-1-yl-acetic acid;
9-p-chlorobenzyl-8-methylthio-1,2,3,4-tetrahydrocar-bazol-1-yl-acetic acid;
9-p-chlorobenzyl-8-methylsulfinyl-1,2,3,4-tetrahydro-carbazol-1-yl-acetic acid;
9-p chlorobenzyl-6-fluoro-3-methyl-1,2,3,4-tetrahydro-carbazol-1-yl-acetic acid;
9-p-chlorobenzyl-6,8-difluoro-1,2,3,4-tetrahydro-carbazol-1-yl-acetic acid;
9-p-chlorobenzyl-6,8-dimethyl-1,2,3,4-tetrahydrocar-bazol-1-yl-acetic acid;
9-p-chlorobenzyl-6-methoxy-8-methyl-1,2,3,4-tetrahydro-carbazol-1-yl-acetic acid;
(-)-9-p-chlorobenzyl-6,8-difluoro-1,2,3,4-tetrahydro-carbazol-1-yl-acetic acid;

(+)-9-p-chlorobenzyl-6,8-difluoro-1,2,3,4-tetrahydro-carbazol-1-yl-acetic acid;
(-)-9-p-chlorobenzyl-8-methyl-1,2,3,4-tetrahydrocar-bazol-1-yl-acetic acid;
(+)-9-p-chlorobenzyl-8-methyl-1,2,3,4-tetrahydrocar-bazol-1-yl-acetic acid;
(-)-9-p-chlorobenzyl-8-fluoro-1,2,3,4-tetrahydrocar-bazol-1-yl-acetic acid;
(+)-9-p-chlorobenzyl-8-fluoro-1,2,3,4-tetrahydrocar-bazol-1-yl-acetic acid;
2-(9-p-chlorobenzyl-6,8-difluoro-1,2,3,4-tetrahydrocar-bazol-1-yl)ethanol;
(-) or (+) 2-(9-p-chlorobenzyl-6,8-difluoro-1,2,3,4-tetrahydrocarbazol-1-yl)ethanol.

6. A compound according to Claim 1, which is:
9-p-chlorobenzyl-6-fluoro-1,2,3,4-tetrahydrocarbazol-1-yl-acetic acid;
9-p-chlorobenzyl-8-methyl-1,2,3,4-tetrahydrocarbazol-1-yl-acetic acid;
9-p-chlorobenzyl-8 fluoro-1,2,3,4-tetrahydrocarbazol-1-yl-acetic acid;
9-p-chlorobenzyl-6,8-difluoro-1,2,3,4-tetrahydro-carbazol-1-yl-acetic acid.

7. A compound according to Claim 1, which is a pure optical isomer.

8. A compound according to Claim 7, which is the (+)-isomer.

9. A compound according to Claim 7, which is the (-)-isomer.

10. A compound according to Claim 9, which is:
(-)9-p-chlorobenzyl-6-fluoro-1,2,3,4-tetrahydro-carbazol-1-yl-acetic acid;
(-)-9-p-Chlorobenzyl-6,8-difluoro-1,2,3,4-tetrahydro-carbazol-1-yl-acetic acid;
(-)-9-p-Chlorobenzyl-8-methyl-1,2,3,4-tetrahydrocar-bazol-1-yl-acetic acid;
(-)-9-p-Chlorobenzyl-8-fluoro-1,2,3,4-tetrahydrocar-bazol-1-yl-acetic acid.

11. A compound of the formula:

wherein:

12. The use of a compound of any one of Claims 1 to 11 for inhibiting leukotriene synthesis in a mammal.

13. The use of a compound of any one of Claims 1 to 11 for antagonizing prostaglandins in mammals.

14. A use according to Claim 13, wherein the prostaglandins are thromboxanes.

15. A pharmaceutical composition which comprises a compound of Claim 1 and a pharm-aceutically acceptable carrier.

16. A pharmaceutical composition which comprises a compound of Claim 1 and an effective amount of a second active ingredient selected from the group consisting of non-steroidal anti-inflammatory drugs; peripheral analgesic agents;
cyclooxygenase inhibitors; leukotriene antagonists;
leukotriene biosynthesis inhibitors; H1-receptor antagonists; H2 receptor antagonists; prostaglandin antagonists; ACE inhibitors, or thromboxane synthetase inhibitors.

17. A pharmaceutical composition according to Claim 16, wherein the second active ingredient is a non-steroidal anti-inflammatory drug.

18. A pharmaceutical composition according to Claim 17, wherein the non-steroidal anti-inflammatory drug is indomethancin.

19. A pharmaceutical composition of Claim 16, wherein the weight ratio of said compound of Claim 1 to said second active ingredient ranges from about 1000:1 to 1:1000.

20. A composition of Claim 19, wherein said ratio is 200:1 to 1:200.

21. A pharmaceutical composition according to Claim 19, wherein the second active ingredient is a non-steroidal anti-inflammatory drug.

22. A pharmaceutical composition according to Claim 21, wherein the non-steroidal anti-inflammatory drug is indomethacin.

23. A pharmaceutical composition which comprises an effective amount of a compound of Claim 5 and an effective amount of a second active ingredient selected from the group consisting of non-steroidal anti-inflammatory drugs; peripheral analgesic agents; cyclooxygenase inhibitors;
leukotriene antagonists; leukotriene biosynthesis inhibitors; H1-receptor antagonists; H2 receptor antagonists; prostaglandin antagonists; ACE
inhibitors, or thromboxane synthetase inhibitors.

24. A pharmaceutical composition according to Claim 23, wherein the weight ratio of said compound of Claim 5 to said second active ingredient ranges from about 1000:1 to 1:1000.

25. A pharmaceutical composition according to Claim 24, wherein said ratio is 200:1 to 1:200.

26. A pharmaceutical composition according to Claim 24, wherein the second active ingredient is a non-steroidal anti-inflammatory drug.

27. A pharmaceutical composition according to Claim 26, wherein the non-steroidal anti-inflammatory drug is indomethacin.