CA1277265C - Cell adhesion peptide - Google Patents

Abstract

CELL ADHESION PEPTIDE

Abstract of the Disclosure The specification discloses a peptide including as the cell-attachment-promoting or cell-attachment-inhibiting constituent the amino acid sequence Arg-Gly-Asp-R wherein R is Ser, Cys, Thr or other amino acid or NH2, and the use of these peptides to alter the cell attachment activity of cells to various substrates. In one aspect the specification discloses a method for promoting cell attachment to a substrate comprising the steps of immobilizing on the substrate a non-naturally occurring polypeptide which includes as the cell-attachment-promoting constituent the sequence -Arg-Gly-Asp-R- wherein R is Ser, Cys, Thr or other amino acid or NH2, such that the polypeptide has cell-attachment-promoting activity; and providing free cells for attachment to said substrate. In another embodiment the present specification discloses a substantially pure peptide including as the cell-attachment-promoting constituent the amino acid sequence Arg-Gly-Asp-R wherein R is Ser, Cys, Thr or other amino acid, or NH2, said peptide having cell-attachment-promoting activity, and said peptide not being a naturally occurring peptide. In still a further embodiment the invention provides a peptide including as the cell-attachment-promoting constituent the amino acid sequence Arg-Gly-Asp-R, wherein R is Ser, Cys, Thr or other amino acid or NH2, such that the peptide has cell-attachment-promoting activity, wherein said peptide has less than about 31 amino acid residues. In still a further embodiment the invention provides a method of inhibiting platelet aggregation comprising providing a non-naturally occurring polypeptide which includes as the cell attachment-promoting constituent the sequence Arg-Gly-Asp-R wherein R is Ser, Cys, Thr or other amino acid or NH2 such that the polypeptide has cell-attachment-promoting activity.

Description

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CELL ADHESION PEPTIDE

This invention is directed to polypeptides related to fibronectin and more particularly to a polypeptide segment of human fibronectin which interacts with cell surfaces. In particular, the invention promotes cell attachment to substrates on which the peptide segment is immobilized, and inhibits cell attachment when presented in solubilized form.
Background of the Invention ~ibronectin is a large glycoprotein, about 450 thousand daltons, which is composed of sever~l apparently independent functional domains. Fibronectin wa~ earlier discovered as a major extracellular matrix protein, and it was demonstrated that it would interact in vitro with other structural molecules, such as collagen, glycosaminoglycans, proteoglycans, fibrinogen, fibrin, and actin, as well as with cell surfaces. It was discovered that fibronectin promotes the attachment of suspended cells to collagen and also that it promotes the attachment of suspended cells directly to tissue culture su~strate, independent of its binding to collagen. Accordingly, investigation continued with respect to the region of the fibronectin molecule that interacts with cell surfaces.
Earlier, a polypeptide fragment of fibronectin which embodies the cell attachment activity of fibronectin was isolated, purified and characterized as a 11.5 kDal polypeptide of 108 amino acid residues, and having the formula: H-Ile-Gly-Gln-Gln-Ser-Thr-Val-Ser-Asp-Val-Pro-Arg-Asp-Leu-Glu-Val-Val-Ala-Ala-Thr-Pro-Thr-Ser-Leu-Leu-Ile-Ser-Trp-Asp-Ala-Pro-Ala-Val-Thr-Val-Arg-Tyr Tyr-Arg-Ile-Thr-Tyr-Gly-Glu-Thr-Gly-Gly-Asn-Ser-Pro-Val-Gln-Glu-Phe-Thr-Val-Pro-Gly-Ser-Lys-Ser-Thr-Ala-Thr-Ile-Ser-Gly-Leu-Lys-Pro-Gly-Val-Asp-Tyr-Thr-Ile-Thr-Val-Tyr-Ala-Val-Thr-Gly-Arg-Gly-Asp-Ser-Pro-Ala-Ser-Ser-Lys-.---.. ~ ~ .

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Pro-Ile-Ser-Ile-Asn-Tyr-Arg-Thr-Glu-Ile-Asp-Lys-Pro-Ser-Gln-Met-OH. Also, a fragment of the foregoing molecule having the same cell attachment activity was synthesized and is comprised of 30 ami~o acid residues having the formula: H-Tyr-Ala-Val-Thr-Gly-Arg-Gly-Asp-Ser-Pro-Ala-Ser-Ser-Lys-Pro-Ile-Ser-Ile-Asn-Tyr-Arg-Thr-Glu-Ile-Asp-Lys-Pro-Ser-Gln-Met-OH. These polypeptides~,-or a fragment thereof which has cell attachment activity, can be used to prepare substrates to which cells will attach. Such sub-~0 strates are useful in cell culture dishes and are also use-ful for employment in medical prosthetic devices for implant-ation in the human body where enhanced cell attachment to the surface is desirabl~.

Summary o the Invention Here we wish to describe the precise localization of this function of the fibronectin molecule as a tetrapeptide se~uence. This sequence or a chemically similar, biologically equivalent, sequence is shared by some other proteins which interact with cells. These incl~de collagen, fibrinogen and surface proteins of E.
coli bac~eria and Sindbis virus. These findings suggest that the mechanism by which cells attach to a fibronectin-containing substrate may be only one instance of a widely general recognition system that cells use to adhere to any number of substrates. This mechanism may also be involved with a cell's phagocytic activity.
Further, it also appears possible that bacteria and possibly even certain viruses may take advantage of this universal cellular adhesion mechanism to gain entry into the body or the cell.
The present invention relates to a peptide including as the cell-attachment-promoting or cell-attachment-inhibiting constituent the amino acid sequence Arg-Gly-Asp-R wherein R is Ser, Cys, Thr or other amino acid or NH2, and the use of these peptides to alter the cell attachment activity of cells to various substrates.

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Thus the present invention provides in one embodiment a method for promotin~ cell attachment to a substrate comprising the steps of immobilizing on the substrate a non-naturally occurring polypeptide which includes as the cell-attachment-promoting constituent the sequence -Arg-Gly-Asp-R- wherein R is Ser, Cys, Thr or other amino acid or NH2, such that the polypeptide has cell-attachment-promoting activity; and providing free cells for attachment to said substrate. ~
lo In another embodiment the present invention provides a substantially pure peptide including as the cell-attachment-promoting constituent the amino acid sequence ~rg-Gly-Asp-R wherein R is Ser, Cys, Thr or other amino acid, or NH2, said peptide having cell-attachment-promoting activity, and said peptide not being a naturally occurring peptide.
In still a further embodiment the invention provides a peptide including as the cell-attachment-promoting constituent the amino acid sequence Arg-Gly-Asp-R, wherein R is Ser, Cys, Thr or other amino acid or NH2, such that the peptide has cell-attachment-promoting activity, wherein said peptide has less than about 31 amino acid residues.
In still a further embodiment the invention provides a method of inhibiting platelet aggregation comprising providing a non-naturally occurring '` polypeptide which includes as the cell attachment-promoting constituent the sequence Arg-Gly-Asp-R wherein R is Ser, Cys, Thr or other amino acid or NH2 such that ` 30 the polypeptide has cell-attachment-promoting activity.
The present invention contemplates a new composition, a polypeptide which alters the cell attachment activity of cells to various substrates independent of its binding to collagen, affects cell phagocytosis, and which consists essentially of an isolated tetrapeptide X-Arg-Gly-Asp-Ser-Y wherein X is H

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or one or more amino acids and Y is O~ or one or more amino acids. The tetrapeptide composition is characterized in that it is substantially isolated from fibronectin, either by separation from fibronectin or by synthesis wherein fibronectin was never pr~sent, and has substantially the same cell attachment activity as fibronectin. In defining the tetrapeptide, there is some variability in one of the amino acids. While Arg-Gly-Asp-Ser is the preferred form of the tetrapeptide of this invention, it may include other amino acids additionally or in a limited sense in substitution for one or more of the amino acids, such as for Ser, Arg-Gly-Asp-Cys or Arg-Gly-Asp-Thr which exhibit a similar cell attachment activity. Chemical moieties may be included at either end, typically at the -COOH end, of the tetrapeptide for the purpose of immobilizing the peptide, or, amino acid additions may be used to modify the cell attachment activity. Also, the invention may be incorporated as part of a larger molecule. The present invention also contemplates the method of using these compositions to promote cell attachment to a substrate wherein the invention is immobilized on the substrate.
The present invention additionally contemplates the method of using the invention in a solubilized or suspended form to inhibit undesirable cell attachment to a substrate or to each other, and to enhance the phagocytic activity of the cells.
Description of the Drawings Figure 1 lists the polypeptides, and their respective amino acid sequence and relative cell attachment activity in concentrations necessary to achieve half-maximal activity, that were synthesized in determining the smallest peptide exhibiting cell attachment activity.
Figure 2 compares cell attachment activity of selected synthesizecl polypeptides from Figure 1 with fibronectin tFN) .

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Figure 3 lists the proteins occurring naturally containing substantially the tetrapeptide sequence of amino acids, the fragment sequence synthesi~ed (in bold type) containing the active site, and the cell attachment activity if tested.
Detailed Description of the Preferred Embodiments Abbreviations for Amino Acids Three-letter One-letter Amino Acid abbreviation symbol Alanine Ala A
Arginine - Arg R
Asparagine Asn ~ N
Aspartic acid Asp D
Cysteine Cys C
Glutamine Gln Q
Glutamic acid Glu E
Glycine Gly G
Histidine His H
Isoleucine Ile Leucine Leu L
Lysine Lys K
Methionine Met M
Phenylalanine Phe F
Proline Pro P
Serine Ser S
Threonine Thr T
Tryptophan Trp W
Tyrosine Tyr Y
Valine Val V
The nomenclature used to define the polypeptide is that specified by Shroder & Lubke, "The Peptides", Academic Press (1965) wherein, in accordance with conventional representation the N-terminus appears to be left, and the C-terminus appears to the right. Where the amino acid residue has isomeric forms, it is the L-form of the amino acid that is represented.
The invention provides a polypeptide having the following ~ormula: H-Arg-Gly-Asp-Ser-OH and is intended to , ~

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include other polypeptides or substances containing this ormula as well as polypeptides formed from the invention by limited substitution or deletion and which have cell attachment activity. (Cell attachment activity hereinafter includes cell attachment promoting activity, phagocytic activity, and the inhibition of cell attachment.) Moreover, the coupling of the peptide to substrates may be faciliated in certain instances, wi~hout affecting the cell attachment activity, by adding a Cys residue at the C-~erminus. Further, the cell attachment activity may be modulated by variable additions to the C- and/or ` N-termini.
The invention, or a larger polypeptide or other molecule including the invention, can be synthesized by any suitable method, such as by exclusively solid-phase technigues, by partial solid-phase techniques, by fragment condensation, or by classical solution addition. It is also envisioned that a hybrid protein with adhesive or cell attachment properties could be generated by combining the peptide of the invention with another protein.
Moreover, using recently developed recombinant DNA
techniques, the invention may be synthesized singularly, or combined with another protein by first including the DNA code of the invention in the code of the desired protein.
Source of Peptides. The peptides are preferably prepared using solid phase synthesis, such as that described by Merrifield, J. Am. Chem. Soc., 85, 2149 (1964), although other equivalent chemical syntheses known in the art, as mentione~ above, can also be used.
Solid-phase synthesis is commenced from the C-terminus of the peptide by co~lpling a protected alpha-amino acid to a suitable resin, as generally set forth in ~.S. Patent No.
4,244,946, issued January 21, 1982 to Rivier et al.
Examples of syntheses of this general type are set forth in U.S. Patents ~os. 4,305,~72 and 4~316,891. Discussion of the solid-phase synthesis of a 41-residue polypeptide ~2~ 6S

is set forth in Science, 213, 1394-1397 (Sept. 1981) in an article by Vale et al., which refers to a more detailed discussion of the synthesis, which appears in an article by Marki et al. in J. Am. Chem. Soc., 103, 3178 (1981).
In order to locate the cell attachment activity of the fibronectin molecule which would possibly be contained within a hydrophilic stretch of amino acids in the 30-amino acid synthetic peptide described above, a number of peptides were synthesized using the Merrifield procedure by Peninsula Laboratories (Belmont, CA), and included, in most cases, a COOH-terminal cystine. The design was to selectively synthesize peptides smaller than a p~evious fragment exhibiting cell attachment activity. As can be clearly seen in Figure 1, this approach permits the determination of the smallest fragment showing activity.
Cell Attachment Promoting Activity. Those peptides having a COOH-terminal cystine residue were assayed for their activity in promoting cell attachment by first attaching them via the heterobifunctional crosslinker SPDP
(N-succinimidyl 3-(2-pyridyldithio) propionate, Sigma, St.
Louis, MO) to rabbit IgG which which was immobilized on plastic. The attachment assay was then carried out as described by Ruoslahti, E. et al., Methods Enzymology 82:803-831 (1981~ using freshly trypsinized normal rat kidney cells (NRK). After a one hour incubation, those cells which had attached were fixed, stained and quantitated using either an Artek Cell counter or a multiscan spectrophotometer. In all cases, maximum attachment was about 80-90% of the cells plated. The relative activity of each peptide is indicated by the concentration of the peptides in nmoles/ml necessary to achieve half-maximal activity, or, where accurate quantitation was not possible, by the crosses at the right of Figure 1, and by the graph in Figure 2.
Among the peptides tested, the tetrapeptide Arg-Gly-Asp-Ser was the smallest one and the only tetrapeptide which, when properly immobilized, had the property of causing cell attachment. The activity of the peptides decreased somewhat with size (Fig. 2). This decrease may be due to a decrease in the stability of their conformation or to a relative inaccessibility on the substrate. The serine residue could be replaced by a cysteine residue without complete loss of the activity of the peptide. When the arginine or aspartic acid residues were selectively deleted, however, the activity was lost.
Moreover, substitution of the glycine with the bulky valine residue also abolished the activity (RVDSPAC, Fig. 1).
Whereas these results show that the residues critical for the activity reside in the tetrapeptide ~sequence, amino acids immediately flanking the four residue fragment may have a modulatory effect on their activity.
It appears that while the tetrapeptide described is the determinant, optimum size of the polypeptide is about a hexapeptide which includes the defined tetrapeptide. Thus, the invention consists essentially of the tetrapeptide which would preferably be part of a hexapeptide.
Arq-Gly-Asp-Ser and Related Sequences in Other Proteins. Having established the importance of the tetrapeptide sequence, a computer search through the published protein sequences was conducted by the National Biomedical Research Foundation (George Washington University Medical Center, Washington, D.C.). Also included were sequences in which the serine was allowed to be replaced by other amino acids, and the arginine and aspartic acid by the chemically similar lysine and glutamic acid, respectively.
3Q The search through other proteins revealed five proteins having the Arg-Gly-Asp-Ser sequence (Figure 3).
Of the five proteins having the identical four amino acid sequence, fibrin(ogen) is of most obvious interest because of its demonstrated interaction with fibronectin and cell surfaces. For this reason, a synthetic nonopeptide designed after the fibrin sequence was tested. As can be seen in Figure 3, it too was an active cell attachment promoter. Attempts to demonstrate ~L2772~i~
the attachment of test cells to intact fibrinogen or fibrin - have given no clear-cut results, although platelets do bind to the fibrinogen molecule.
Among the remaining proteins that possess sequences related to the active tetrapeptide, the family of collagenous proteins is o:E particular interest for two reasons. First, collagens have been shown to mediate cell attachment independently of fibronectin, and, secondly, variations of the tetrapeptide sequence are particularly abundant in collagens. These sequences are repeated at fairly regular intervals along the ~1 and ~2 chains of type I collagen.
Four synthetic peptides were prepared based on diverse sequences in collagen. In three of these, the serine residue was replaced by threonine, hydroxy proline, or lysine, respectively. In the fourth one a lysine was substituted for arginine and glutamic acid for aspartic acid. Of these peptides only the serine to threonine substitution was active with the NRK test cells (Figure 2).
Other chemically similar substitutions such as serine to alanine may also yield active sites.
The results show that tne primary cell-binding site of the fibronectin molecule resides in an extremely short amino acid sequence which is shared by at least one other adhesive protein, collagen. Because of the nature of the proteins having the tetrapeptide sequence among the proteins searched, the result suggest that the tetrapeptide with selected substitutions, may represent a universal attachment mechanism.
Inhibition of_Cell Attachment. If the mechanism of cell attachment involves the recognition of the amino acid sequence of the tetrapeptide by a receptor on the cell, then it could be postulated that attachment could be inhibited by preventing this recognition by "blocking" the receptor. To demonstrate this inhibitory function of the invention, fibronectin was immobilized on a substrate to be tested for cell attachment activity. In separate experiments, various concentrations of the tetrapeptide ~L2t77~6~i (Arg-Gly-Asp-Ser) and a hexapeptide, Pro-Arg-Gly-Asp-Ser-Gly in a solubilized form were combined with the free cells, and attachment activity measured as above. Both peptides were S shown to inhibit the normal attachment of cells to a fibronectin-coated substrate when placed in a solubilized form in combination with the free cells. The concentration necessary to exhibit half-maximal cell inhibition activity was 0.6-0.8 mmoles/ml and 0.3 mmoles/ml for the tetrapeptide and the hexapeptide, respectively.
Enhanced Phaqoc~tic Activity. Fibronectin has been shown to promote phagocytosis, and this activity has been linked to the cell attachment activity. An application that can be envisioned for the invention based on these observations is to promote the entrance to cells of particles containing, for example, a therapeutic agent, by administering the particles with the invention in a solubilized form.
Practical application such as the preparation of surfaces for optimal cell culture, the derivatization of various prosthetic materials to promote bonding with surrounding tissues, a method to provide for the increased in-ternalization of molecules such as toxins, drugs, hormones, or the like by the enhancement of phagocytosis, and the development of ways of manipulating cellular adhesion mechanisms in diseases such as cancer metastasis and platelet aggregation can also be envisioned. Since a peptide of four amino acids is unlikely to have more than one binding site, one question that can be addressed now is whether the interaction of all types of cells with fibronectin involves this same region of the molecule.
Platelets, for example, may bind fibronectin on their surfaces by a different mechanism. This would be important in using this peptide to regulate cell attachment or in the design of prosthetic materials. It would also shed light on the role played by fibronectin in vivo.
In particular, the coating of the culture \~

~2~2~;5 substrate with the cell-attachment polypeptide obviates the use of fibronectin in the medium, thus providing better defined conditions for the culture as well as better reproducibility. As one example of commercial use of cell-attachment surfaces, "Cytodex" particles, manufactured by Pharmacia, are coated with gelatin, making it possible to grow the same number of adherçnt cells in a much smaller volume of medium than would be possible in dishes. The activity of these beads is generally dependent upon the use of fibronectin in the growth medium, and the - cell-attachment polypeptide is expected to provide an improved, chemically-defined coating for such purposes.
Other surfaces or materials may be coated to enhance attachment, such as glass, agarose, synthetic resins, or long-chain polysaccharides.
Medical devices can be designed making use of such substrata to attract cells to the surface in vivo or even to promote the growing of a desired cell type on a particular surface prior to grafting. An example of such an approach is the induction of endothelial cell growth on a prosthetic blood v~ssel or vascular graft, which is generally woven or knitted from nitrocellulose or polyester fiber, particularly "Dacron"` (polyethylene terephtha]ate) fiber. Most types of cells are attracted to fibronectin and to this polypeptide, but endothelial cells and fibroblastic cells in particular are attracted to fibronectin. The latter point indicates the potential usefulness of-this defined polypeptide in coating a patch graft or the like for aiding wound closure and healing following an accident or surgery. In such cases, it may be advantageous to couple the peptide ~o a biological molecule, such as collagen, a glycosaminoglycan or a proteoglycan; for example, the five-residue fragment having a Cys-residue at the C-terminus coupled to monomeric collagen by using a crosslinker such as 3-(2-pyridyldithio) propionic acid N-hydroxysuccinimide ester to effect cross-linking of the cysteine to a lysine residue in the collagen, or by using carbodiimide without * Trademark ~2~726~

the Cys on the four-residue fragment. It is also indicative of its value in coating surfaces of a prosthetic device which is intended to s~rve as a temporary or semipermanent enl:ry into the body, e.g. into a blood vessel or into the peritoneal cavity, sometimes referred to as a percutaneous device. Fibronectin has been shown to be chemotactic to fibroblasts and macrophages.
This activity correlates with the presence of the cell attachment domain. One potential manifestation of the cell attachment activity of the synthetic peptides described here, and fragments thereof of like characteristics, is chemotactic activity.
Application of the ability of the invention to inhibit cell attachment when presented in a solubilized form may find utility in situations where it is desirable to prevent cell adhesion to a substrate or adhesion between cells. Undesirable cell attachment to various medical sut~res, or dressings, may be prevented by presen~ing the invention in solubilized form. The invent-ion therefore, may be applied in the form of a liquid, lotion, salve, gel, colloid or powder. When the invention is used either in conjunction or combination with another molecule, such as a therapeutic agent, or particle treated with such an agent, the en~rance of that agent or particle into the cell may be enhanced by the e~fect of the invention on the phagocytic activity of the cell, thereby increasing the efficiency of the therapeutic a~ent.
Although ~he inventi~ll h~s been described with regard to certain preferred embodiments, it should be understood that various changes and modifications, as would be obvious to one having the ordinary skill in this art, may be made without departing from the scope of the invention which is defined in the appended claims. For example, it may not be necessary to have the free acid at the C-terminus, as it may be amidated or substituted by some ~ther group. Further, limited substitutions may be made to the basic tetrapeptide as illustrated by the substitution of Ser by Cy~, without destroying the inherent cell attachment activity. Particular features of ~27726~

the invention are emphasized in the claims which follow.
Industrial Application The invention is useful in surgery and therapeutic reconstruction and treatment of injuries.

Claims (32)

THE EMBODIMENTS OF THE INVENTION IN WHICH AN EXCLUSIVE
PROPERTY OR PRIVILEGE IS CLAIMED ARE DEFINED AS FOLLOWS:

1. A method for promoting cell attachment to a substrate comprising the steps of:
immobilizing on the substrate a non-naturally occurring polypeptide which includes as the cell-attachment-promoting constituent the sequence -Arg-Gly-Asp-R- wherein R is chosen from the group consisting of amino acids and NH2, such that the polypeptide has cell-attachment-promoting activity; and providing free cells for attachment to said substrate.

2. A method for promoting cell attachment to a substrate comprising the steps of:
immobilizing on the substrate a non-naturally occurring polypeptide X-Arg-Gly-Asp-R-Y
wherein X is H or at least one amino acid and Y is OH or at least one amino acid, and R is chosen from the group consisting of amino acids and NH2, such that said polypeptide has cell-attachment-promoting activity; and providing free cells for attachment to the substrate.

3. A method of promoting cell attachment to a substrate, comprising the steps of:
immobilizing on the substrate a synthetic polypeptide which includes as the cell-attachment-promoting constituent the amino acid sequence Arg-Gly-Asp-R wherein R is chosen from the group consisting of amino acids and NH2, such that the polypeptide has cell attachment promoting activity, said polypeptide having less than 31 amino acid residues; and providing free cells for attachment to said substrate.

4. A method of promoting cell attachment to a substrate, comprising the steps of:
immobilizing on the substrate a synthetic polypeptide X-Arg-Gly-Asp-R-Y
wherein X is H or at least one amino acid and Y is OH or at least one amino acid, and R is chosen from the group consisting of amino acids and NH2, such that said peptide has cell attachment promoting activity, said polypeptide having less than about 31 amino acid residues; and providing free cells for attachment to said substrate.

5. A synthetic peptide including the amino acid sequence Arg-Gly-Asp-R, wherein R is chosen from the group consisting of amino acids and NH2, said peptide having cell attachment-promoting activity when immobilized on a substrate and cell detachment-promoting activity when in solubilized form, said peptide not being a naturally occurring peptide.

6. A composition of matter comprising the peptide of claim 5 attached to a substrate.

7. A synthetic peptide including as the cell-attachment-promoting constituent the amino acid sequence Arg-Gly-Asp-R, wherein R is chosen from the group consisting of amino acids and NH2, said peptide having cell attachment-promoting activity when immobilized on a substrate and cell detachment-promoting activity when in solubilized form, and wherein said peptide has less than 31 amino acid residues.

8. A composition of matter comprising the peptide of claim 7 attached to a substrate.

9. The composition of matter as claimed in any one of claims 6 or 8, wherein the peptide is attached to the surface of a prosthetic device.

10. The composition of matter as claimed in any one of claims 6 or 8, wherein the peptide is attached to the surface of a synthetic resin fiber.

11. The composition of matter as claimed in any one of claims 6 or 8, wherein the peptide is attached to the surface of a percutaneous device.

12. The composition of matter as claimed in any one of claims 6 or 8, wherein the peptide is attached to the surface of a solid substrate to ensure that cells will attach to said substrate surface.

13. The composition of matter as claimed in any one of claims 6 or 8, wherein the peptide is attached to the surface of a solid substrate to ensure that cells will attach to said substrate surface and wherein said substrate is glass, synthetic resin or long-chain polysaccharide.

14. The composition of matter as claimed in any one of claims 6 or 8, wherein the peptide is attached to the surface of a solid substrate to ensure that cells will attach to said substrate surface and wherein the substrate is nitrocellulose or polyester.

15. The composition of matter as claimed in any one of claims 6 or 8, wherein the peptide is attached to the surface of a solid substrate to ensure that cells will attach to said substrate surface and wherein the substrate is agarose.

16. The composition of matter as claimed in any one of claims 6 or 8, wherein the peptide is attached to the surface of a solid substrate to ensure that cells will attach to said substrate surface, wherein the substrate is collagen.

17. The composition of matter as claimed in any one of claims 6 or 8, wherein the peptide is attached to the surface of a solid substrate to ensure that cells will attach to said substrate surface, wherein it is coupled to and forms part of a vascular graft.

18. The composition of matter as claimed in any one of claims 6 or 8, wherein the peptide is in the form of a lotion, salve, gel, colloid or powder.

19. A method of inhibiting platelet aggregation in vitro comprising providing a non-naturally occurring polypeptide in solubilized form which includes the sequence Arg-Gly-Asp-R, wherein R is chosen from the group consisting of amino acids and NH2, such that the polypeptide inhibits the attachment of platelet cells to fibrinogen.

20. A method of inhibiting platelet aggregation in vitro comprising providing a synthetic polypeptide in solubilized form having less than 31 amino acid residues which includes the amino acid sequence Arg-Gly-Asp-R, wherein R is chosen from the group consisting of amino acids and NH2, such that the polypeptide inhibits the attachment of platelet cells to fibrinogen.

21. The use of a non-naturally occurring polypeptide in solubilized form including the amino acid sequence Arg-Gly-Asp-R, wherein R is chosen from the group consisting of amino acids and NH2, such that the polypeptide, in solubilized form, inhibits the attachment of platelet cells to fibrinogen, for inhibiting platelet aggregation.

22. The use of a synthetic polypeptide in solubilized form having less than 31 amino acids which includes the amino acid sequence Arg-Gly-Asp-R, wherein R is chosen from the group consisting of amino acids and NH2, such that the polypeptide inhibits the attachment of platelet cells to fibrinogen, for inhibiting platelet aggregation.

23. The method as claimed in claim 1 wherein R is NH2 or chosen from the group consisting of Ser, Thr or Cys.

24. The method as claimed in claim 2 wherein R is chosen from the group consisting of Ser, Thr or Cys.

25. The method as claimed in claim 3 wherein R is NH2 or chosen from the group consisting of Ser, Thr or Cys.

26. The method as claimed in claim 4 wherein R is chosen from the group consisting of Ser, Thr or Cys.

27. The peptide as claimed in claim 5 wherein R is NH2 or chosen from the group consisting of Ser, Thr or Cys.

28. The peptide as claimed in claim 7 wherein R is NH2 or chosen from the group consisting of Ser, Thr or Cys.

29. The method as claimed in claim 19 wherein R is NH2 or chosen from the group consisting of Ser, Thr or Cys.

30. The method as claimed in claim 20 wherein R is NH2 or chosen from the group consisting of Ser, Thr or Cys.

31. The use as claimed in claim 21 wherein R is NH2 or is chosen from the group consisting of Ser, Thr or Cys.

32. The use as claimed in claim 22 wherein R is NH2 or is chosen from the group consisting of Ser, Thr or Cys.