CA1267621C - Method and cell line for obtaining plasminogen activators - Google Patents

Method and cell line for obtaining plasminogen activators

Info

Publication number
CA1267621C
CA1267621C CA477723A CA477723A CA1267621C CA 1267621 C CA1267621 C CA 1267621C CA 477723 A CA477723 A CA 477723A CA 477723 A CA477723 A CA 477723A CA 1267621 C CA1267621 C CA 1267621C
Authority
CA
Canada
Prior art keywords
cell line
plasminogen activator
plasminogen activators
adapting
disclosed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
CA477723A
Other languages
French (fr)
Other versions
CA1267621A (en
Inventor
Sham Y Chan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US06/704,593 external-priority patent/US4757005A/en
Application filed filed Critical
Priority to CA000477723A priority Critical patent/CA1267621A/en
Priority to CA000615619A priority patent/CA1291055C/en
Application granted granted Critical
Publication of CA1267621C publication Critical patent/CA1267621C/en
Publication of CA1267621A publication Critical patent/CA1267621A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/91—Cell lines ; Processes using cell lines

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

ABSTRACT OF THE DISCLOSURE A method of increasing the yield of plasminogen activator from a plasminogen activator-producing cell line by adapting said cell line to grow in a suitable growth medium essentially free of fetal calf serum is disclosed. Also disclosed are cell lines capable of producing relatively large quantities of plasminogen activator, and a chemically defined growth medium suitable for adapting a plasminogen activator-secreting cell line to growth in the absence of fetal calf serum. MS-1338-CIP
CA000477723A 1984-04-19 1985-03-28 Method and cell line for obtaining plasminogen activators Expired CA1267621A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CA000477723A CA1267621A (en) 1984-04-19 1985-03-28 Method and cell line for obtaining plasminogen activators
CA000615619A CA1291055C (en) 1984-04-19 1990-01-23 Method and cell line for obtaining plasminogen activators

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US60186884A 1984-04-19 1984-04-19
US601,868 1984-04-19
US06/704,593 US4757005A (en) 1984-04-19 1985-02-22 Method and cell line for obtaining plasminogen activators
US704,593 1985-02-22
CA000477723A CA1267621A (en) 1984-04-19 1985-03-28 Method and cell line for obtaining plasminogen activators

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA000615619A Division CA1291055C (en) 1984-04-19 1990-01-23 Method and cell line for obtaining plasminogen activators

Publications (2)

Publication Number Publication Date
CA1267621C true CA1267621C (en) 1990-04-10
CA1267621A CA1267621A (en) 1990-04-10

Family

ID=27167505

Family Applications (2)

Application Number Title Priority Date Filing Date
CA000477723A Expired CA1267621A (en) 1984-04-19 1985-03-28 Method and cell line for obtaining plasminogen activators
CA000615619A Expired - Lifetime CA1291055C (en) 1984-04-19 1990-01-23 Method and cell line for obtaining plasminogen activators

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA000615619A Expired - Lifetime CA1291055C (en) 1984-04-19 1990-01-23 Method and cell line for obtaining plasminogen activators

Country Status (1)

Country Link
CA (2) CA1267621A (en)

Also Published As

Publication number Publication date
CA1267621A (en) 1990-04-10
CA1291055C (en) 1991-10-22

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Legal Events

Date Code Title Description
MKLA Lapsed
MKLA Lapsed

Effective date: 19990412