CA1267621C - Method and cell line for obtaining plasminogen activators - Google Patents
Method and cell line for obtaining plasminogen activatorsInfo
- Publication number
- CA1267621C CA1267621C CA477723A CA477723A CA1267621C CA 1267621 C CA1267621 C CA 1267621C CA 477723 A CA477723 A CA 477723A CA 477723 A CA477723 A CA 477723A CA 1267621 C CA1267621 C CA 1267621C
- Authority
- CA
- Canada
- Prior art keywords
- cell line
- plasminogen activator
- plasminogen activators
- adapting
- disclosed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/91—Cell lines ; Processes using cell lines
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
ABSTRACT OF THE DISCLOSURE A method of increasing the yield of plasminogen activator from a plasminogen activator-producing cell line by adapting said cell line to grow in a suitable growth medium essentially free of fetal calf serum is disclosed. Also disclosed are cell lines capable of producing relatively large quantities of plasminogen activator, and a chemically defined growth medium suitable for adapting a plasminogen activator-secreting cell line to growth in the absence of fetal calf serum. MS-1338-CIP
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA000477723A CA1267621A (en) | 1984-04-19 | 1985-03-28 | Method and cell line for obtaining plasminogen activators |
CA000615619A CA1291055C (en) | 1984-04-19 | 1990-01-23 | Method and cell line for obtaining plasminogen activators |
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US60186884A | 1984-04-19 | 1984-04-19 | |
US601,868 | 1984-04-19 | ||
US06/704,593 US4757005A (en) | 1984-04-19 | 1985-02-22 | Method and cell line for obtaining plasminogen activators |
US704,593 | 1985-02-22 | ||
CA000477723A CA1267621A (en) | 1984-04-19 | 1985-03-28 | Method and cell line for obtaining plasminogen activators |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA000615619A Division CA1291055C (en) | 1984-04-19 | 1990-01-23 | Method and cell line for obtaining plasminogen activators |
Publications (2)
Publication Number | Publication Date |
---|---|
CA1267621C true CA1267621C (en) | 1990-04-10 |
CA1267621A CA1267621A (en) | 1990-04-10 |
Family
ID=27167505
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA000477723A Expired CA1267621A (en) | 1984-04-19 | 1985-03-28 | Method and cell line for obtaining plasminogen activators |
CA000615619A Expired - Lifetime CA1291055C (en) | 1984-04-19 | 1990-01-23 | Method and cell line for obtaining plasminogen activators |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA000615619A Expired - Lifetime CA1291055C (en) | 1984-04-19 | 1990-01-23 | Method and cell line for obtaining plasminogen activators |
Country Status (1)
Country | Link |
---|---|
CA (2) | CA1267621A (en) |
-
1985
- 1985-03-28 CA CA000477723A patent/CA1267621A/en not_active Expired
-
1990
- 1990-01-23 CA CA000615619A patent/CA1291055C/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
CA1267621A (en) | 1990-04-10 |
CA1291055C (en) | 1991-10-22 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
MKLA | Lapsed | ||
MKLA | Lapsed |
Effective date: 19990412 |