CA1246873A - Method of inoculating mushroom basidiospores, seed basidiospore bed for inoculation, culture container for seed basidiospore bed, and boring apparatus for host wood for inoculation - Google Patents
Method of inoculating mushroom basidiospores, seed basidiospore bed for inoculation, culture container for seed basidiospore bed, and boring apparatus for host wood for inoculationInfo
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- CA1246873A CA1246873A CA000457950A CA457950A CA1246873A CA 1246873 A CA1246873 A CA 1246873A CA 000457950 A CA000457950 A CA 000457950A CA 457950 A CA457950 A CA 457950A CA 1246873 A CA1246873 A CA 1246873A
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- basidiospore
- bed
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Abstract
Abstract A method of inoculating basidiospores of mushroom (Lentinus edodes) is disclosed. At least one transverse saw cut is made on the exterior surface of a piece of host wood with a suitable depth, thereby forming an inoculation recess. A substantially flat, generally half-moon shaped piece of wood with seed basidiospores cultured thereon, known as a seed basidiospore bed is inserted into the inoculation recess. A culture container comprising a capped half-split screwed bottle-like body is suitable for incubation and storage of seed basidiospore beds. A cutting apparatus, designed to efficiently cut several transverse inoculation recesses in the host wood simultaneously by the use of multiple saws on swingable arms is also disclosed.
Description
~6~73 25326-8 The present invention relates to a method of inoculating mushroom basidiospores, a seed basidiospore bed for inoculation, a culture container for the seed basidiospore bed, and a cutting apparatus for the host wood for inoculation.
In order to artificially cultivate mushrooms, especially Lentinus edodes for edible use, a trunk of a certain broad-leaved tree (e.g., oak, maple, birch, etc.) is divided into suitable lengths to make a host wood for inoculation of basidiospores. The outer circum-ferential surfaces of the host wood are cut to make inoculation recesses, into which seed basidiospore beds obtained by pure culture of the seed basidiospores inoculated on wood pieces are set, and cultivation occurs.
Conventional methods for inoculating mushroom basidiospores include a method of inserting a wedge-form seed basidiospore bed into a planting space provided by cutting a host wood with a chisel, and a method of stuffing a sawdust culture of basidiospore or a rod form seed basidiospore bed into a round hole in a host wood provided by an electric drill. These methods have generally been in practice.
The problem with the aforementioned conventional methods is that to provide the number of planting holes on the host wood necessary to obtain a high planting density, and so as to enable the spawn to spread rapidly in the host wood after inoculation, much time and labour is required to inoculate the seed basidiospores in each plantillg hole.
The present inventioll utili~st}le fact that the hyp]la, foilowing inoculation on the host wood for cultivation of mushrooms, will multiply at a rate many times faster in the lengthwise direction of wood than in the widthwise direction of wood.
~'24~f~73 It is an object of the present invention to provide a new seed basidiospore inoculation method. A saw cutout in the host wood, from its surface to a suitable depth in a transverse direction of said wood is provided, to form a recess along the outer circumferential surface of tlle host wood. A flat half-moon shaped seed basidiospore bed fitted into the recess in a manner to block said inoculation recess, allows the spawn to develop quickly from the seed basidiospore bed and rapidly spread into the host wood over a broad range. This results in a sparse density of inoculation,which elevates the efficiency of inoc-ulation work, accelerates the crop time and increases the yield, with suppression of miscellaneous bacterial propagation.
Another object of the present invention is to provide a seed basidiospore bed for inoculation suitable for this new inoculation method, and a culture container for the seed basidiospore bed suitable for culture.
A further object of the present invention is to provide a cutting apparatus for the host wood for inoculation, and by mechanization of the formation of inoculation recesses, making it possible to carry out the work of cutting the recesses in a short time.
In accordance with the invention there is provided a method of inoculating basidiospores of mushrooms ~Lentinus edodes) comprising making at least one saw cut on an exterior surEace oE a l~iece ot ilOSt wood, the saw cut extending in a transverso direction with respect to the piece of host wood and to a suitable depth thereby forming a transverse inoculation recess in the outer surface and inserting in the recess a substantially flat, generally half-moon shaped seed basidiospore bed in a manner to substantially fill said recess.
In drawings which illustrate embodiments of the invention, Figure 1 is an illustrative view showing the condition of multiplication and propagation of mushroom spawn in the host wood according to the conventional method;
Figure 2 is an illustrative view showing the condition of multiplication and propagation of spawn in the host wood according to the present invention;
Figure 3 is a perspective view showing the condition of inoculation of the seed basidiospore bed according to the present invention;
Figure 4(a) to 4(f) are plan views showing various forms of the seed basidiospore bed to be used in the present invention;
Figure 5 is a side view of the seed basidiospore bed to be used in the present invention;
Figure 6 is a perspective view of the seed basidiospore bed to be used in the present invention, partly provided with a coated layer;
Figure 7 is a perspective view of a wood plece which forms a medium for the seed basidiospore bed to be used in the present invention, provided with a number of artificial fissures;
Figure 8 is a front view, partly cut away, of a culture container for culture of a secd basidiospore bcd to be used :in the present invention;
Figure 9 is a front view, partly cut away, of the culture container accordillg to Figure 8, shown in a disassembled state;
~2~73 Figure lO is a schematic front view, partly cut away of a cutting apparatus for the host wood for inoculation to be used in the present invention;
Figure ll is a plan view illustrating the condition of use of the apparatus of Figure lO; and, Figure 12 is a front view illustrating the condition of formation of the recess for inoculation on the host wood using the above cutting apparatus.
~ s shown in Figures l and 2, the seed basidiospore bed 2, inoculated on the host wood M propagates in the lengthwise direction (vertical direction) Y of the wood at a rate many times faster than that in the breadthwise direction X of the wood. In the present invention, a saw cut is given to the host wood M to a suitable depth in the widthwise direction X thereof, so as to provide an inoculation recess 1 having a transversely long cutout 1' along the outer periphery of the host wood M, as shown in Figure 3. Into this inoculation recess 1 a flat, half-moon shaped seed basidiospore bed 2 is set in a manner to close the cut end 1' with the arc-shaped periphery 5 of the seed basidiospore bed 2. The basidiospore inoculated on the host wood M
via the seed basidiospore bed 2 multiplies, as shown in Figure 2, in a very broad range in comparison with the conventional method of Figure 1, thus making use of the characteristic of the seed bllsidiosporc to propagate rapidly in a lengtllwise direction Y as previously described.
Accordingly, it is possible to reduce the number of pieces of the seed basidiospore 2 to be inoculated to far fewer than that of the conventional metllod, lea~ing to a remarkable curtailment oflabour and time for the ~2~6~73 inoculation work. Also, according to the present invention, spawn of the basidiospore spreads evenly and rapidly in the host wood M, so that propagation of foreign bacteria is suppressed, and simultaneously, the period from inoculation to mushroom development is shortened.
Moreover, during cultivation, the mushrooms are apt to develop simul-taneously from the surface of the host wood, so that it is possible to expect acceleration of crop time and increase in yield.
The seed basidiospore bed 2 to be used in the present invention is made of a wood piece having a thickness nearly equal to that of the cut end 1' of the inoculation recess 1 to be formed as aforementioned, and cut out into a half-moon shape to conform to the profile of the inoculation recess. The seed basidiospores make a pure culture thereon. Desirably, the seed basidiospore bed 2 is to be buried in the host wood M so as to fill up the gap in inoculating.
Practically, however, the host wood M has various diameters and its sectional size is not fixed. Therefore, the seed basidiospore bed 2 which is produced on a standard basis does not always conform with the cut end 1' of the host wood M and the inoculation recess 1.
Accordingly, the present invention has been designed to pro~ide a notch on the inner periphery of the seed basidiospore bed
In order to artificially cultivate mushrooms, especially Lentinus edodes for edible use, a trunk of a certain broad-leaved tree (e.g., oak, maple, birch, etc.) is divided into suitable lengths to make a host wood for inoculation of basidiospores. The outer circum-ferential surfaces of the host wood are cut to make inoculation recesses, into which seed basidiospore beds obtained by pure culture of the seed basidiospores inoculated on wood pieces are set, and cultivation occurs.
Conventional methods for inoculating mushroom basidiospores include a method of inserting a wedge-form seed basidiospore bed into a planting space provided by cutting a host wood with a chisel, and a method of stuffing a sawdust culture of basidiospore or a rod form seed basidiospore bed into a round hole in a host wood provided by an electric drill. These methods have generally been in practice.
The problem with the aforementioned conventional methods is that to provide the number of planting holes on the host wood necessary to obtain a high planting density, and so as to enable the spawn to spread rapidly in the host wood after inoculation, much time and labour is required to inoculate the seed basidiospores in each plantillg hole.
The present inventioll utili~st}le fact that the hyp]la, foilowing inoculation on the host wood for cultivation of mushrooms, will multiply at a rate many times faster in the lengthwise direction of wood than in the widthwise direction of wood.
~'24~f~73 It is an object of the present invention to provide a new seed basidiospore inoculation method. A saw cutout in the host wood, from its surface to a suitable depth in a transverse direction of said wood is provided, to form a recess along the outer circumferential surface of tlle host wood. A flat half-moon shaped seed basidiospore bed fitted into the recess in a manner to block said inoculation recess, allows the spawn to develop quickly from the seed basidiospore bed and rapidly spread into the host wood over a broad range. This results in a sparse density of inoculation,which elevates the efficiency of inoc-ulation work, accelerates the crop time and increases the yield, with suppression of miscellaneous bacterial propagation.
Another object of the present invention is to provide a seed basidiospore bed for inoculation suitable for this new inoculation method, and a culture container for the seed basidiospore bed suitable for culture.
A further object of the present invention is to provide a cutting apparatus for the host wood for inoculation, and by mechanization of the formation of inoculation recesses, making it possible to carry out the work of cutting the recesses in a short time.
In accordance with the invention there is provided a method of inoculating basidiospores of mushrooms ~Lentinus edodes) comprising making at least one saw cut on an exterior surEace oE a l~iece ot ilOSt wood, the saw cut extending in a transverso direction with respect to the piece of host wood and to a suitable depth thereby forming a transverse inoculation recess in the outer surface and inserting in the recess a substantially flat, generally half-moon shaped seed basidiospore bed in a manner to substantially fill said recess.
In drawings which illustrate embodiments of the invention, Figure 1 is an illustrative view showing the condition of multiplication and propagation of mushroom spawn in the host wood according to the conventional method;
Figure 2 is an illustrative view showing the condition of multiplication and propagation of spawn in the host wood according to the present invention;
Figure 3 is a perspective view showing the condition of inoculation of the seed basidiospore bed according to the present invention;
Figure 4(a) to 4(f) are plan views showing various forms of the seed basidiospore bed to be used in the present invention;
Figure 5 is a side view of the seed basidiospore bed to be used in the present invention;
Figure 6 is a perspective view of the seed basidiospore bed to be used in the present invention, partly provided with a coated layer;
Figure 7 is a perspective view of a wood plece which forms a medium for the seed basidiospore bed to be used in the present invention, provided with a number of artificial fissures;
Figure 8 is a front view, partly cut away, of a culture container for culture of a secd basidiospore bcd to be used :in the present invention;
Figure 9 is a front view, partly cut away, of the culture container accordillg to Figure 8, shown in a disassembled state;
~2~73 Figure lO is a schematic front view, partly cut away of a cutting apparatus for the host wood for inoculation to be used in the present invention;
Figure ll is a plan view illustrating the condition of use of the apparatus of Figure lO; and, Figure 12 is a front view illustrating the condition of formation of the recess for inoculation on the host wood using the above cutting apparatus.
~ s shown in Figures l and 2, the seed basidiospore bed 2, inoculated on the host wood M propagates in the lengthwise direction (vertical direction) Y of the wood at a rate many times faster than that in the breadthwise direction X of the wood. In the present invention, a saw cut is given to the host wood M to a suitable depth in the widthwise direction X thereof, so as to provide an inoculation recess 1 having a transversely long cutout 1' along the outer periphery of the host wood M, as shown in Figure 3. Into this inoculation recess 1 a flat, half-moon shaped seed basidiospore bed 2 is set in a manner to close the cut end 1' with the arc-shaped periphery 5 of the seed basidiospore bed 2. The basidiospore inoculated on the host wood M
via the seed basidiospore bed 2 multiplies, as shown in Figure 2, in a very broad range in comparison with the conventional method of Figure 1, thus making use of the characteristic of the seed bllsidiosporc to propagate rapidly in a lengtllwise direction Y as previously described.
Accordingly, it is possible to reduce the number of pieces of the seed basidiospore 2 to be inoculated to far fewer than that of the conventional metllod, lea~ing to a remarkable curtailment oflabour and time for the ~2~6~73 inoculation work. Also, according to the present invention, spawn of the basidiospore spreads evenly and rapidly in the host wood M, so that propagation of foreign bacteria is suppressed, and simultaneously, the period from inoculation to mushroom development is shortened.
Moreover, during cultivation, the mushrooms are apt to develop simul-taneously from the surface of the host wood, so that it is possible to expect acceleration of crop time and increase in yield.
The seed basidiospore bed 2 to be used in the present invention is made of a wood piece having a thickness nearly equal to that of the cut end 1' of the inoculation recess 1 to be formed as aforementioned, and cut out into a half-moon shape to conform to the profile of the inoculation recess. The seed basidiospores make a pure culture thereon. Desirably, the seed basidiospore bed 2 is to be buried in the host wood M so as to fill up the gap in inoculating.
Practically, however, the host wood M has various diameters and its sectional size is not fixed. Therefore, the seed basidiospore bed 2 which is produced on a standard basis does not always conform with the cut end 1' of the host wood M and the inoculation recess 1.
Accordingly, the present invention has been designed to pro~ide a notch on the inner periphery of the seed basidiospore bed
2 which is as a whole formed in a flat, half-moon shape, which enables the size of the seed basidiospore bed 2 to bc Inodif:ied to conforlll to the size of the inoculcltion recess 1.
The seed basidiospore bed 2 whose size is made variable is shown in Figure 4. In the figure, the part 3 is a notch provided on the seed basidiospore bed 2 from the mid part of its inner periphery 4 Gf~7;3~
(or the side to go inside the inoculation recess 1) toward the mid part of the arc periphery 5 (which is formed to fill up the cut end 1' of the inoculation recess 1). This notch 3 may be formed, as shown in Figure 4(a), in the style of a small width slit, or, as shown in Figure 4~b), in a style of an isosceles triangle cutout, or~ as shown in Figure 4(c), in a style of an isosceles triangle cutout wi~h an arc at the top, or, as shown in Figure 4(d), by being cut through to the periphery 5 of the arc, so that the seed basidiospore bed ' to be set into an inoculation recess 1 is substantially constituted into a pair of symmetrical pieces 21 and 22. In any of these styles, the individual seed basidiospore bed 2 which has been uniformly produced can be modified by further opening or closing the notch 3 so as to bring it near to the size and the shape of the individual inoculation recess 1 which differs according to the size of the diameter of the host wood M to which the bed is applied. (Refer to Figure 3).
With respect to the shape of the inoculation recess 1 in the-present invention, the width and the height of the cut end 1' differ little even by a change in the kind of the saw to be used, but the shape of the inner profile may vary. For example, when a straight tooth type saw is used~ the cut end becomes a straight line form, however when a circular tooth saw is used, the cut end becomes an arc form. Accordingly, the present invention :is designed to covc:r modifications of a seed basidiosporc bed 2 having a linear inner peri-phery 4 as shown in Figure 4(e), and a seed basidiospore bed 2 having an arc inner periphery 4 opposite to the arc periphery 5 as shown in l1igure 4(f). These two kinds of seed basidiospore bed 2 6~373 can be selectively used according to the kind of saw to be used so that the seed basidiospore bed 2 can be exactly set into the inner periphery of the inoculation recess 1.
~ urther, as shown in Figure 5, the present invention is designed to facilitate insertion of the seed basidiospore bed 2 into the inoculation recess 1 by tapering its thickness toward the inside end 4 so as to become slightly thinner than the thickness of the outer end 5 of the arc. In this case, if the wall of the seed basidiospore bed 2 is uniformly tapered, the arc periphery 5 which is to block the cutout 1' of the inoculation recess 1 leaves spaces at its two ends proportionate to the tapering angle. Therefore, to facilitate insertion from the froPt of the inoculation recess 1, it is desi.rable to form the seed basidiospore bed 2 in a reversed conically inclined surface in a manner to make the mid part of the inner periphery 4 which is received by the cut end 1' the thinnest. A seed basidiospore bed which is formed in a wedge form so that the vertical section at the mid part of the seed basidiospore bed 2 is smaller in the direction of advance into the inoculation recess 1 can attain the object of the present invention. According to the present invention designed as above, in inoculating the basidiospore bed in the host wood M, first the thin inner periphery 4 is inserted into the inoculation recess 1, so that the insertion is easily made. Tllerefore, in inocula~illg tlle flat, rather fragile, seed bas:idiospore bed 2, brcakage of the bed 2 is prevented and the work efficiency improved.
The present invention is further designed, as shown in Figure 6? to provide in advance a chemical coating layer 6 or a covering layer on the arc periphery 5 of the seed basidiospore bed 2 which is to be exposed on the cut end 1' after the inoculation for prevention of entry of foreign bacteria or rain water. This can eliminate the after-treatment work which follows the inoculation of the seed basidio-spore bed 2. As the arc periphery 5 of the seed basidiospore bed 2 is exposed on the surface of the host wood M blocking the cut end 1' of the inoculation recess 1, foreign bacteria and rain water are apt to come in from the exposed portion, and also the exposed portion gives out water. Accordingly, it is desirable to apply wax or water-proof film onto the exposed portion. However, such treatment requires extra time, and it is often omitted, giving rise to one of the causes of defective cultivations. According to the present invention wherein the portion to be exposed is previously provided with a coating layer 6 or a covering layer as above, the necessary treatment can be done simultaneously with the planting of the seed basidiospore bed 2. The coating layer 6 is formed by applying, before culture of the seed basidiospores, an acrylic or urethane or silicone resin solely or with incorporation with 0.5 to 0.1 % of butyl benzoa~e or thiabendazol or methyl-l-~butylcarbamoyl)-2-benzimidazol carbamate as a mold preventing agent. As a modified embodiment of the present invention, a thin synthetic resin film containing the above component may be a~plied to make a coating layer.
~urther, tl~e present invelltion is designed, as SllOWn in ~igure 7, to form a number of artificial fissures 9 on the wood piece 2' (to become the medium for the seed basidiospore bed, before culture of the seed spore~ in tlle direction of cross grain 8 of said wood ~2~ 73 piece 2'. ~his makes it possible to shorten the culture duration of the seed basidiospore bed 2 ~Id realize full ripening more rapidly.
Formation of artificial fissures 9 on the wood piece 2' cln be made by allowing the grain 8 to pass through the rotating roll on which a plurality of circular sawblades are coaxially mounted in such a manner that the grain 8 crosses the axial line of said roll. When the wood piece 2' provided artificially with the fissures 9 is seeded with the seed basidiospores and cultivated, the characteristics of the spores of the kind which are apt to propagate in vertical direction can be utilizedJ thus shortening the culture duration, and an early full ripening can be attained. It is thus possible to offer an excellent seed basidiospore bed 2 which is rich in active deposition property and easy to cultivate.
According to the present invention, the half-moon shaped seed basidiospore bed 2 to be used in the present invention can be readily taken in and out of the culture container, so that the seed basidiospore bed 2 which is prone to cracking due to its flat shape, can be prevented from being damaged. Furthermore, the empty culture containers can be kept in storage without occupying a large space.
Referring to the culture container A of the present invention, as shown in Figures 8 and 9, a thin walled, hollow synthctic resin culture containc-~r body A, havillg a mouth 10 to acconlmodate a cap B
at its upyer end, is divicled into the upper and the lower half parts at the lower part of the shoulder 11 of the container A, i.e., into the upper section Al and the lower section A2 which are mutually readily engageable and separable.
~2~6~3 The upper section Al is ~urnished with a mouth 10, which is screwed at its upper end with a double tube type cap B for plugging with cotton (not shown in the drawing~, a shoulder 11 whose circumfer-ential size is enlarged from the lower end of the mouth 10, and a cylindrical coupling part 12 which is continued to the lower section of the shoulder 11 with the same diameter. The coupling part 12 has on its inner circumferential surface the concave sectional circumferential grooves 131, 132 in the two upper and the lower steps. The lower step circumferential groove 132 has a wedge form concave section.
Also, the lower edge 12' of the coupling part 12 is formed into a slightly outwardly open edge. On the other hand, the lower section A2 has an open circular upper periphery 14', and at its top end a coupling part 14 which is to be engaged in tight contact with the inner circumferential surface of the coupling part 12.
Downward from the coupling part 14, a barrel 15 and a bottom 16 of the container are formed in one-piece. On the outer circumferential surface of the coupling part 14 there are the circumferential convex sectional ribs 171, 172 having the corresponding shapes respectively to the above circumferential grooves 131 and 132, and, on a part continued to the barrel 15, at the lower edge 12' of the coupling part 12, a stopping flange 18 to restrict the coupling depth is provicled.
In the contailler body A for cultivLItillg the sced basidiosporc bed constituted as al)ove, there are contained the flat, half-moon shaped wood pieces 2' (of Figure 7) which are to form the materials for the seed basidiospore bed 2 together witl- the nutrient suitablc for cultivating the mushroom spores, and the coupling part 12 is set 9.2~7 3 into the coupling part 14 until the lower edge 12' of the upper section Al is stopped by the stopper flange 18 of the lower section A2, by which the circumferential grooves 131, 132 are engaged respectively with the circumferential ribs 171, 172, with the result that the lower section A2 is prevented from slipping out even when the bottle is carried with its mouth 10 held. Then, through the mouth 10 mushroom spores are seeded on the above wood piece 2', a cotton-plugged cap B set in, and cultivation is made in the culture chamber for a pre-scribed period of time. The seed basidiospore bed 2 which has completed culture of the seed spores, under the state contained in the container body A, is carried to the place of cultivation where the host wood M for inoculation is available, taken out by removing the upper section Al, and inoculated according to the procedures as aforedescribed.
The culture container of the present invention constituted as above and made usable as described, can be used to store the material ~ood piece 2' and to take out the seed basidiospore bed 2 in planting, by largely opening the container. Therefore, it is suitable for contain-ing the flat, fragile seed basidiospore beds and is effective for preventing the beds from being damaged. The container can be safely carried. In keeping the empty containers in stock, the upper and the lower container sections Al and A2 can bc la.icl :in comb:inLItioll respoctivcly to reduce their total volume.
The transverse inoculation recess 1 to bc formed on the host wood M fQr inoculatipn to he used in the present invention is, according to the present invention, scarcely affected by the size or the bend of the raw wood, and can be mechanically cut almost uniformly and with high eficiency.
The cutting apparatus for the host wood for inoculation of the present invention with reference to Figures 10, 11, and 12, will now be described.
In Figure 10, the cutting apparatus includes a frame F and a hearing stand 20. The bearing stand 20 comprises three Y-shaped stays 20a erecte~l at a certain interval along the center line of the frame F and rollers 20b which are accommodated respectively to the upper right and left parts of the stays 20a. By means of three pairs of rollers 20b which are inclined in a V-shape, the host wood M is supported at its front and rear parts and middle part. A plurality of arms 21, each being supported by the fulcrum shaft 21' provided on the frame F, are freely swingably provided on the transverse direction of the host wood M. A circular sawblade 22 for cutting the host wood M transversely is fixed to the tip of each arm 21 in freely rotatable manner. Each blade 22 is linked via the pulley 23~ driving belt 24, pulley 25 provided on the fulcrum 21' of the arm, and the motor rotation shaft 27 for driving the pulley 25 via the driving belt 26 transversally laid on the frame F. Each sawblade rotates following the motor ~not illustrated) which drives the motor rotation shaft 27.
Coil springs 28 are provided betwecn the frame r: and the respective arms 21. A tractive device 29 exerts a swing mot:ion to each arm 21 resisting the coil spring 28 so as to bring the sawblade 22 close to the host wood M. This tractive device 29 comprises a wire 29a whose one end is fixed to the arm 21 with its middle part supported by ~L2~37~
the roller 30J and a coi] spring type elastic member 29b connected with the other end of the wire 29a. A cutting limiter 31 restricts the depth of cutting of the sawblade 22 in contact with the surface of the host wood M. Each cutting limiter 31 has a slot 31' at its base of fitting to the arm, so that, by sliding this slot 31' along the stopping screw 32, the distance between the lirrliter 31 and the crest of the sawblade 22 is changed to adjust the cutting depth of the saw-blade 22 In the embodiment as shown in Figure 11, the arms 21 (ten in total) are arranged in a spiral manner to surround the bearing stand 20 from all directions each being furnished with a coil spring, a sawblade, a tractive device, and a cutting limiter. As illustrated in Figure 10, the lower end of the tractive device 29 (coil spring type elastiç member 29b) is fixed to either of the three unit pedals with its lower end projected to the front lower part of the frame F. On treading the pedal 33r shown at the bottom of Figure 10, the arms 213, 216, and 211o positioned on the right side in Figure 11 are tracted. When the central pedal 33 is treaded, likewise the other arms 217 and 219 on the right side and the arms 212 and 214 on the left side are tracted, while on the other hand, when the left side pedal 331 is treaded, likewise the other left side arms 211, 215, and 218 are tracted. Each arm 21 is co~ ectocl resl~ectively with a single pedal 33, beiJIg ctivicled into tl~ree directiorls of right side, up-down side, and left side, according to the direction of access to the host wood M. Also, each pedal 33rJ c~ and 1 is provided on the reverse surface at the foremost end as shown in Figure 10, with a through ~13~
~2~ 73 hole 34 on the mutually common position, and, by thrusting a single rod 35 therethrough which i5 freely detachably engaged therewith, the abovementioned 10 arms 21 are si~lltaneously *racted.
The above 10 arms 211 10 have, as shown in Figure 10, their fulcrum positions and lengths suitably, and, as shown in Figure 11, they are suitably arranged at nearly an equal distance along the lengthwise direction of the host wood M. The sawblades 221 10 at the tip of each arm 211 10 are, as shown in Figure 12, so provided as to make it possible to cut at nearly an equal angle to each other into the circumference of the host wood M. Also, the sawblades 221 10 are so provided that the cutting position of the inoculation recesses 11 10 to be formed in a transverse direction of the surface of the host wood are to be arranged on nearly two round spiral lines.
In Figure 11, the portion shown in the solid line of khe inoculation recesses 11 10 is that visible from above. The portion shown in the broken line represents the portion to be formed on the reverse side. The short lines at both ends are to show the approximate length of the recess.
The aforementioned motor rotation shafts 27 for driving the `~ 20 saw~lades 22 are two parallel horizontal units along both sides of the bearing stand 20, having mutually a relation o~ convcying motive power. One shaft is conncctcd with ~m o~ltpUt shaft of a motor or an engine.
In using the present cutting apparatus constituted as above, the tracting device 29 is activated to bring the arm 21 close to the host wood M supyorted by the bearing stand 20. Then the sawblade 22 ~14-~;246~73 driven by the motor rotation shaft 27 cuts into the host wood M in a transverse direction. The depth of cutting is determined in such manner that the cutting limiter 31, together with the sawblade 22, having an access to and in contact with the surface of the host wood M, fixes the angle of the arm 21. The traction force which works beyond it is absorbed by an elongation of the elastic member 29b which is attached to the tracting device 29. Accordingly, both in the case of individually tracting the arm 21 and in the case of simultan-eously tracting the arm 21 to form the inoculation recesses 11 10' the cutting depth, i.e., the depth of the inoculation recess, can be formed at a nearly constant rate. Also, even in the case of some differences in the diameters~ or existence or not of bend of the host wood M, the inoculation recesses are formed at the prescribed positions with a nearly fixed depth.
As described above, according to the present invention, even in the case of differences in coarseness or inclusion or not of bend in the host wood, the inooulation recess 1 to be provided can be mechanically formed with a suitable depth. Also, according to the present invention, it is possible to keep a piece of host wood placed at a fixed position, to which various inoculation recesses 1 can be provided at a certain interval with one another and from diversified directions. Moreover, according to the present invention, plural inoculation recesses 1 can be provided simultancously in the s~le direction on a single host wood M by means of a single pedal operation.
Further, according to the present invention, it is possible to form at a time the prescrihed number of inoculation recesses 1 on ~15 ~
~6~73 the surface of a piece of host wood M at a certain distance and with a spiral arrangement around the host wood. Furthermore, according to the present in~ention, by changing the setting position of the cutting limiter 31, the depth of the inoculation recess 1 can be adjusted according to the si~e of the host wood M.
The seed basidiospore bed 2 whose size is made variable is shown in Figure 4. In the figure, the part 3 is a notch provided on the seed basidiospore bed 2 from the mid part of its inner periphery 4 Gf~7;3~
(or the side to go inside the inoculation recess 1) toward the mid part of the arc periphery 5 (which is formed to fill up the cut end 1' of the inoculation recess 1). This notch 3 may be formed, as shown in Figure 4(a), in the style of a small width slit, or, as shown in Figure 4~b), in a style of an isosceles triangle cutout, or~ as shown in Figure 4(c), in a style of an isosceles triangle cutout wi~h an arc at the top, or, as shown in Figure 4(d), by being cut through to the periphery 5 of the arc, so that the seed basidiospore bed ' to be set into an inoculation recess 1 is substantially constituted into a pair of symmetrical pieces 21 and 22. In any of these styles, the individual seed basidiospore bed 2 which has been uniformly produced can be modified by further opening or closing the notch 3 so as to bring it near to the size and the shape of the individual inoculation recess 1 which differs according to the size of the diameter of the host wood M to which the bed is applied. (Refer to Figure 3).
With respect to the shape of the inoculation recess 1 in the-present invention, the width and the height of the cut end 1' differ little even by a change in the kind of the saw to be used, but the shape of the inner profile may vary. For example, when a straight tooth type saw is used~ the cut end becomes a straight line form, however when a circular tooth saw is used, the cut end becomes an arc form. Accordingly, the present invention :is designed to covc:r modifications of a seed basidiosporc bed 2 having a linear inner peri-phery 4 as shown in Figure 4(e), and a seed basidiospore bed 2 having an arc inner periphery 4 opposite to the arc periphery 5 as shown in l1igure 4(f). These two kinds of seed basidiospore bed 2 6~373 can be selectively used according to the kind of saw to be used so that the seed basidiospore bed 2 can be exactly set into the inner periphery of the inoculation recess 1.
~ urther, as shown in Figure 5, the present invention is designed to facilitate insertion of the seed basidiospore bed 2 into the inoculation recess 1 by tapering its thickness toward the inside end 4 so as to become slightly thinner than the thickness of the outer end 5 of the arc. In this case, if the wall of the seed basidiospore bed 2 is uniformly tapered, the arc periphery 5 which is to block the cutout 1' of the inoculation recess 1 leaves spaces at its two ends proportionate to the tapering angle. Therefore, to facilitate insertion from the froPt of the inoculation recess 1, it is desi.rable to form the seed basidiospore bed 2 in a reversed conically inclined surface in a manner to make the mid part of the inner periphery 4 which is received by the cut end 1' the thinnest. A seed basidiospore bed which is formed in a wedge form so that the vertical section at the mid part of the seed basidiospore bed 2 is smaller in the direction of advance into the inoculation recess 1 can attain the object of the present invention. According to the present invention designed as above, in inoculating the basidiospore bed in the host wood M, first the thin inner periphery 4 is inserted into the inoculation recess 1, so that the insertion is easily made. Tllerefore, in inocula~illg tlle flat, rather fragile, seed bas:idiospore bed 2, brcakage of the bed 2 is prevented and the work efficiency improved.
The present invention is further designed, as shown in Figure 6? to provide in advance a chemical coating layer 6 or a covering layer on the arc periphery 5 of the seed basidiospore bed 2 which is to be exposed on the cut end 1' after the inoculation for prevention of entry of foreign bacteria or rain water. This can eliminate the after-treatment work which follows the inoculation of the seed basidio-spore bed 2. As the arc periphery 5 of the seed basidiospore bed 2 is exposed on the surface of the host wood M blocking the cut end 1' of the inoculation recess 1, foreign bacteria and rain water are apt to come in from the exposed portion, and also the exposed portion gives out water. Accordingly, it is desirable to apply wax or water-proof film onto the exposed portion. However, such treatment requires extra time, and it is often omitted, giving rise to one of the causes of defective cultivations. According to the present invention wherein the portion to be exposed is previously provided with a coating layer 6 or a covering layer as above, the necessary treatment can be done simultaneously with the planting of the seed basidiospore bed 2. The coating layer 6 is formed by applying, before culture of the seed basidiospores, an acrylic or urethane or silicone resin solely or with incorporation with 0.5 to 0.1 % of butyl benzoa~e or thiabendazol or methyl-l-~butylcarbamoyl)-2-benzimidazol carbamate as a mold preventing agent. As a modified embodiment of the present invention, a thin synthetic resin film containing the above component may be a~plied to make a coating layer.
~urther, tl~e present invelltion is designed, as SllOWn in ~igure 7, to form a number of artificial fissures 9 on the wood piece 2' (to become the medium for the seed basidiospore bed, before culture of the seed spore~ in tlle direction of cross grain 8 of said wood ~2~ 73 piece 2'. ~his makes it possible to shorten the culture duration of the seed basidiospore bed 2 ~Id realize full ripening more rapidly.
Formation of artificial fissures 9 on the wood piece 2' cln be made by allowing the grain 8 to pass through the rotating roll on which a plurality of circular sawblades are coaxially mounted in such a manner that the grain 8 crosses the axial line of said roll. When the wood piece 2' provided artificially with the fissures 9 is seeded with the seed basidiospores and cultivated, the characteristics of the spores of the kind which are apt to propagate in vertical direction can be utilizedJ thus shortening the culture duration, and an early full ripening can be attained. It is thus possible to offer an excellent seed basidiospore bed 2 which is rich in active deposition property and easy to cultivate.
According to the present invention, the half-moon shaped seed basidiospore bed 2 to be used in the present invention can be readily taken in and out of the culture container, so that the seed basidiospore bed 2 which is prone to cracking due to its flat shape, can be prevented from being damaged. Furthermore, the empty culture containers can be kept in storage without occupying a large space.
Referring to the culture container A of the present invention, as shown in Figures 8 and 9, a thin walled, hollow synthctic resin culture containc-~r body A, havillg a mouth 10 to acconlmodate a cap B
at its upyer end, is divicled into the upper and the lower half parts at the lower part of the shoulder 11 of the container A, i.e., into the upper section Al and the lower section A2 which are mutually readily engageable and separable.
~2~6~3 The upper section Al is ~urnished with a mouth 10, which is screwed at its upper end with a double tube type cap B for plugging with cotton (not shown in the drawing~, a shoulder 11 whose circumfer-ential size is enlarged from the lower end of the mouth 10, and a cylindrical coupling part 12 which is continued to the lower section of the shoulder 11 with the same diameter. The coupling part 12 has on its inner circumferential surface the concave sectional circumferential grooves 131, 132 in the two upper and the lower steps. The lower step circumferential groove 132 has a wedge form concave section.
Also, the lower edge 12' of the coupling part 12 is formed into a slightly outwardly open edge. On the other hand, the lower section A2 has an open circular upper periphery 14', and at its top end a coupling part 14 which is to be engaged in tight contact with the inner circumferential surface of the coupling part 12.
Downward from the coupling part 14, a barrel 15 and a bottom 16 of the container are formed in one-piece. On the outer circumferential surface of the coupling part 14 there are the circumferential convex sectional ribs 171, 172 having the corresponding shapes respectively to the above circumferential grooves 131 and 132, and, on a part continued to the barrel 15, at the lower edge 12' of the coupling part 12, a stopping flange 18 to restrict the coupling depth is provicled.
In the contailler body A for cultivLItillg the sced basidiosporc bed constituted as al)ove, there are contained the flat, half-moon shaped wood pieces 2' (of Figure 7) which are to form the materials for the seed basidiospore bed 2 together witl- the nutrient suitablc for cultivating the mushroom spores, and the coupling part 12 is set 9.2~7 3 into the coupling part 14 until the lower edge 12' of the upper section Al is stopped by the stopper flange 18 of the lower section A2, by which the circumferential grooves 131, 132 are engaged respectively with the circumferential ribs 171, 172, with the result that the lower section A2 is prevented from slipping out even when the bottle is carried with its mouth 10 held. Then, through the mouth 10 mushroom spores are seeded on the above wood piece 2', a cotton-plugged cap B set in, and cultivation is made in the culture chamber for a pre-scribed period of time. The seed basidiospore bed 2 which has completed culture of the seed spores, under the state contained in the container body A, is carried to the place of cultivation where the host wood M for inoculation is available, taken out by removing the upper section Al, and inoculated according to the procedures as aforedescribed.
The culture container of the present invention constituted as above and made usable as described, can be used to store the material ~ood piece 2' and to take out the seed basidiospore bed 2 in planting, by largely opening the container. Therefore, it is suitable for contain-ing the flat, fragile seed basidiospore beds and is effective for preventing the beds from being damaged. The container can be safely carried. In keeping the empty containers in stock, the upper and the lower container sections Al and A2 can bc la.icl :in comb:inLItioll respoctivcly to reduce their total volume.
The transverse inoculation recess 1 to bc formed on the host wood M fQr inoculatipn to he used in the present invention is, according to the present invention, scarcely affected by the size or the bend of the raw wood, and can be mechanically cut almost uniformly and with high eficiency.
The cutting apparatus for the host wood for inoculation of the present invention with reference to Figures 10, 11, and 12, will now be described.
In Figure 10, the cutting apparatus includes a frame F and a hearing stand 20. The bearing stand 20 comprises three Y-shaped stays 20a erecte~l at a certain interval along the center line of the frame F and rollers 20b which are accommodated respectively to the upper right and left parts of the stays 20a. By means of three pairs of rollers 20b which are inclined in a V-shape, the host wood M is supported at its front and rear parts and middle part. A plurality of arms 21, each being supported by the fulcrum shaft 21' provided on the frame F, are freely swingably provided on the transverse direction of the host wood M. A circular sawblade 22 for cutting the host wood M transversely is fixed to the tip of each arm 21 in freely rotatable manner. Each blade 22 is linked via the pulley 23~ driving belt 24, pulley 25 provided on the fulcrum 21' of the arm, and the motor rotation shaft 27 for driving the pulley 25 via the driving belt 26 transversally laid on the frame F. Each sawblade rotates following the motor ~not illustrated) which drives the motor rotation shaft 27.
Coil springs 28 are provided betwecn the frame r: and the respective arms 21. A tractive device 29 exerts a swing mot:ion to each arm 21 resisting the coil spring 28 so as to bring the sawblade 22 close to the host wood M. This tractive device 29 comprises a wire 29a whose one end is fixed to the arm 21 with its middle part supported by ~L2~37~
the roller 30J and a coi] spring type elastic member 29b connected with the other end of the wire 29a. A cutting limiter 31 restricts the depth of cutting of the sawblade 22 in contact with the surface of the host wood M. Each cutting limiter 31 has a slot 31' at its base of fitting to the arm, so that, by sliding this slot 31' along the stopping screw 32, the distance between the lirrliter 31 and the crest of the sawblade 22 is changed to adjust the cutting depth of the saw-blade 22 In the embodiment as shown in Figure 11, the arms 21 (ten in total) are arranged in a spiral manner to surround the bearing stand 20 from all directions each being furnished with a coil spring, a sawblade, a tractive device, and a cutting limiter. As illustrated in Figure 10, the lower end of the tractive device 29 (coil spring type elastiç member 29b) is fixed to either of the three unit pedals with its lower end projected to the front lower part of the frame F. On treading the pedal 33r shown at the bottom of Figure 10, the arms 213, 216, and 211o positioned on the right side in Figure 11 are tracted. When the central pedal 33 is treaded, likewise the other arms 217 and 219 on the right side and the arms 212 and 214 on the left side are tracted, while on the other hand, when the left side pedal 331 is treaded, likewise the other left side arms 211, 215, and 218 are tracted. Each arm 21 is co~ ectocl resl~ectively with a single pedal 33, beiJIg ctivicled into tl~ree directiorls of right side, up-down side, and left side, according to the direction of access to the host wood M. Also, each pedal 33rJ c~ and 1 is provided on the reverse surface at the foremost end as shown in Figure 10, with a through ~13~
~2~ 73 hole 34 on the mutually common position, and, by thrusting a single rod 35 therethrough which i5 freely detachably engaged therewith, the abovementioned 10 arms 21 are si~lltaneously *racted.
The above 10 arms 211 10 have, as shown in Figure 10, their fulcrum positions and lengths suitably, and, as shown in Figure 11, they are suitably arranged at nearly an equal distance along the lengthwise direction of the host wood M. The sawblades 221 10 at the tip of each arm 211 10 are, as shown in Figure 12, so provided as to make it possible to cut at nearly an equal angle to each other into the circumference of the host wood M. Also, the sawblades 221 10 are so provided that the cutting position of the inoculation recesses 11 10 to be formed in a transverse direction of the surface of the host wood are to be arranged on nearly two round spiral lines.
In Figure 11, the portion shown in the solid line of khe inoculation recesses 11 10 is that visible from above. The portion shown in the broken line represents the portion to be formed on the reverse side. The short lines at both ends are to show the approximate length of the recess.
The aforementioned motor rotation shafts 27 for driving the `~ 20 saw~lades 22 are two parallel horizontal units along both sides of the bearing stand 20, having mutually a relation o~ convcying motive power. One shaft is conncctcd with ~m o~ltpUt shaft of a motor or an engine.
In using the present cutting apparatus constituted as above, the tracting device 29 is activated to bring the arm 21 close to the host wood M supyorted by the bearing stand 20. Then the sawblade 22 ~14-~;246~73 driven by the motor rotation shaft 27 cuts into the host wood M in a transverse direction. The depth of cutting is determined in such manner that the cutting limiter 31, together with the sawblade 22, having an access to and in contact with the surface of the host wood M, fixes the angle of the arm 21. The traction force which works beyond it is absorbed by an elongation of the elastic member 29b which is attached to the tracting device 29. Accordingly, both in the case of individually tracting the arm 21 and in the case of simultan-eously tracting the arm 21 to form the inoculation recesses 11 10' the cutting depth, i.e., the depth of the inoculation recess, can be formed at a nearly constant rate. Also, even in the case of some differences in the diameters~ or existence or not of bend of the host wood M, the inoculation recesses are formed at the prescribed positions with a nearly fixed depth.
As described above, according to the present invention, even in the case of differences in coarseness or inclusion or not of bend in the host wood, the inooulation recess 1 to be provided can be mechanically formed with a suitable depth. Also, according to the present invention, it is possible to keep a piece of host wood placed at a fixed position, to which various inoculation recesses 1 can be provided at a certain interval with one another and from diversified directions. Moreover, according to the present invention, plural inoculation recesses 1 can be provided simultancously in the s~le direction on a single host wood M by means of a single pedal operation.
Further, according to the present invention, it is possible to form at a time the prescrihed number of inoculation recesses 1 on ~15 ~
~6~73 the surface of a piece of host wood M at a certain distance and with a spiral arrangement around the host wood. Furthermore, according to the present in~ention, by changing the setting position of the cutting limiter 31, the depth of the inoculation recess 1 can be adjusted according to the si~e of the host wood M.
Claims (20)
PROPERTY OR PRIVILEGE ARE DEFINED AS FOLLOWS:
1. A method of inoculating basidiospores of mushroom (Lentinus edodes) comprising making at least one saw cut on an exterior surface of a piece of host wood, the saw cut extending in a transverse direction with respect to the piece of host wood and to a suitable depth thereby forming a transverse inoculation recess in the outer surface and inserting in the recess a substantially flat, generally half-moon shaped seed basidiospore bed in a manner to substantially fill said recess.
2. A seed basidiospore bed to be used in the method of inoculation according to Claim 1, wherein the seed basidiospore bed comprises a substantially flat, half-moon shaped piece of wood having a convex arcuate edge and an opposite edge, with seed basidiospores cultured on the piece of wood.
3. The seed basidiospore bed according to Claim 2, wherein said seed basidiospore bed includes a notch of an appropriate depth in the mid part of the opposite edge.
4. The seed basidiospore bed according to Claim 3, wherein said notch is formed like an isosceles triangle having an appropriate bottom side length.
5. The seed basidiospore bed according to Claim 4, wherein said notch has an arc at the top of the isosceles triangle.
6. The seed basidiospore bed according to Claim 3, wherein said notch reaches the middle part of the periphery of the arcuate edge, by which the seed basidiospore bed comprises substantially a unit of a pair of right and left side pieces.
7. The seed basidiospore bed according to Claim 2, wherein said opposite edge of the seed basidiospore bed is formed as a straight line, said bed being as a whole formed in a flat comb-like shape in plan view.
8. The seed basidiospore bed according to Claim 2, wherein said opposite edge is also convex arcuate.
9. The seed basidiospore bed according to Claim 2, wherein said arcuate edge has a thickness nearly equal to the cutout of said recess for inoculation, said opposite edge has a thickness thinner than that of said arcuate edge, and at least the upper sectional surface passing through the mid part of the two sides is inclined in a wedge form so as to become thinner along the direction of entry into said recess for inoculation.
10. The seed basidiospore bed according to Claim 2, wherein the arcuate edge of said seed basidiospore bed, prior to inoculation, is provided with a coated layer or a covered layer to prevent foreign bacteria or rainwater from entering after the inoculation, and to prevent excess moisture loss from the seed basidiospore bed.
11. The seed basidiospore bed according to Claim 2, wherein said bed has on one of its flat surfaces a number of artificial fissures provided in a direction which intersects the grain of the wood piece.
12. A container for culture of the seed basidiospore bed according to Claim 2, which comprises a thin-walled hollow cylindrical container body having a mouth for accommodating a cap at the top, said container body being separable into half of the upper and the lower sections, the upper section including a mouth and a shoulder part, said upper section having a cylindrical form with its diameter equal to that of the container body and its circular lower end opened, including on the outer periphery of the cylindrical coupling part a convex sectional circumferential groove, and said lower section having a cylindrical form with its circular upper end opened, including on the outer periphery of the cylindrical coupling part a convex sectional circum-ferential rib to engage with said circumferential groove, a barrel and a bottom of the container body being formed in one-piece continuous with the underside of said coupling part.
13. The culture container for the seed basidiospore bed according to Claim 12, wherein said circumferential grooves and circumferential ribs are formed in more than two steps on the upper and the lower parts respectively on said coupling parts and, one step each thereof is formed in convex and concave wedge forms in section respectively so as to act as a stopgap between the female coupling part and the male coupling part.
14. The culture container for the seed basidiospore bed according to Claim 13, wherein there is formed a stopping flange which restricts the mutual coupling depth in contact with the lower edge of the coupling part of said upper section on the part having the continuation of the coupling part and the barrel part of said lower section, so that the lower edge of said coupling part in contact with said stopping flange is formed into an open edge which is slightly flared outwardly.
15. A cutting apparatus for making saw cuts in host wood for use in the method according to Claim 1 comprising a bearing stand for supporting the host wood and a plurality of swingable arms which swing in a direction transverse to the host wood, each arm provided with a sawblade a tractive device, and a cutting limiter, wherein the saw blade cuts the host wood supported on the bearing stand in a transverse direction, the tractive device for positioning the arm comprises a coil spring type elastic member attached to a wire, and the cutting limiter restricts the depth of cutting of the saw blade in the host wood.
16. The cutting apparatus for host wood according to Claim 15, wherein the plurality of arms having said, sawblade, tractive device furnished with an elastic member and a cutting limiter are provided at nearly equal intervals in the lengthwise direction of the host wood supported by said bearing stand, and the arms are arranged around said bearing stand from the circumferential direction of the host wood so that the sawblade of each arm shall approach at nearly equal angles to one another.
17. The cutting apparatus for host wood according to Claim 15, wherein the tractive devices provided on each arm are connected with a pedal.
18. The cutting apparatus for host wood for inoculation according to Claim 15, wherein said pedals may be connected in series by a single connecting rod which is accommodated in a freely disengageable manner in a position common with one another, so that the pedals may operate simultaneously.
19. The cutting apparatus for host wood according to Claim 15, wherein said plurality of arms are arranged around said bearing stand at nearly equal intervals in the lengthwise direction of the host wood and in a manner such that the respective blades are arranged in the circumferential direction of the host wood at nearly the same angle to each other, characterized in that the cuts of said sawblades will be arranged on a spiral locus to the center of the host wood.
20. The cutting apparatus for host wood according to Claim 15, wherein said cutting limiter is fitted to said arm in such a manner that advances and retractions in the direction of cutting of the sawblade are adjustable through a slot and a stopping screw.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA000457950A CA1246873A (en) | 1984-06-29 | 1984-06-29 | Method of inoculating mushroom basidiospores, seed basidiospore bed for inoculation, culture container for seed basidiospore bed, and boring apparatus for host wood for inoculation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA000457950A CA1246873A (en) | 1984-06-29 | 1984-06-29 | Method of inoculating mushroom basidiospores, seed basidiospore bed for inoculation, culture container for seed basidiospore bed, and boring apparatus for host wood for inoculation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1246873A true CA1246873A (en) | 1988-12-20 |
Family
ID=4128222
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000457950A Expired CA1246873A (en) | 1984-06-29 | 1984-06-29 | Method of inoculating mushroom basidiospores, seed basidiospore bed for inoculation, culture container for seed basidiospore bed, and boring apparatus for host wood for inoculation |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA1246873A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111359914A (en) * | 2020-01-18 | 2020-07-03 | 山西大学 | Device and method for screening crop seeds |
-
1984
- 1984-06-29 CA CA000457950A patent/CA1246873A/en not_active Expired
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111359914A (en) * | 2020-01-18 | 2020-07-03 | 山西大学 | Device and method for screening crop seeds |
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