CA1201169A - Method and apparatus for measuring gas partial pressure in living body - Google Patents
Method and apparatus for measuring gas partial pressure in living bodyInfo
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- CA1201169A CA1201169A CA000426165A CA426165A CA1201169A CA 1201169 A CA1201169 A CA 1201169A CA 000426165 A CA000426165 A CA 000426165A CA 426165 A CA426165 A CA 426165A CA 1201169 A CA1201169 A CA 1201169A
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Abstract
ABSTRACT OF THE DISCLOSURE
A method and apparatus for measuring a gas partial pressure in a living body on the basis of the principle of polarography. A gas partial pressure sensor includes a biomedical electrode inserted in the living body. A temperature sensor is further provided to measure a temperature of the living body. A polarographic current value measured by the gas partial pressure sensor is corrected to a current value at a reference temperature depending on the temperature measured by the temperature sensor. Then the gas partial pressure at the reference temperature is determined based on a working curve which represents a relationship between the corrected polarographic current value and the gas partial pressure at the reference temperature.
A method and apparatus for measuring a gas partial pressure in a living body on the basis of the principle of polarography. A gas partial pressure sensor includes a biomedical electrode inserted in the living body. A temperature sensor is further provided to measure a temperature of the living body. A polarographic current value measured by the gas partial pressure sensor is corrected to a current value at a reference temperature depending on the temperature measured by the temperature sensor. Then the gas partial pressure at the reference temperature is determined based on a working curve which represents a relationship between the corrected polarographic current value and the gas partial pressure at the reference temperature.
Description
1 This invention relates to a method and apparatus for measuring the concentration of various kinds of gases existing in biological tissue and biological fluid, especially, blood, the method being one of measures adapted ~or quantitatively gathering biological conditions in the field of medical science.
In particular, the present invention relates to a method and apparatus for measuring a partial pressure in a living body on the basis of the polarography.
Two major methods have hitherto been available for the gas concentration or partial pres-sure measurements.
A first method is based on the so-called spot measurement wherein blood is extracted from a living body and thereafter the gas concentration is measured through a chemical analysis process.
A second method is such that a small sensor is inserted into a living body and the gas concentration in terms of an electrical signal is measured.
Disadvantageously, the ~irst method consumes much time until a result is obtained and besides, blood being in process of extraction from the living bod~ tends to come into contact with ambient air, and gas exchan~e will be caused between the extracted blood and the ambient aix with the result l that a measured value representative of a gas concentration in the llving body tends to be inaccurate.
The second method, on the other hand, is excellent which can provide instantaneous results, thus facilitating on-line monitoring of a living body condition. This method has therefore been proposed in various ways mainly on the basis of polarographic process. This method utilizing an electrode reaction, however, faces a problem of temperature dependency in which current or voltage values obtained greatly depend on temperatures and accurate values can not be obtained unless the living body is kept at a constant temperature.
For example, a sensor for measuring the oxygen concentration in liquid usually has a cathode made of a noble metal such as gold, platinum, silver or the like and an anode, such as an Ag/AgCl electrode.
Oxygen is reduced at the surface of the cathode and a polarographic current which has a small current value due to the reducing reaction is measured.
Since the current value varies with an oxygen concentration in the liquid and temperature of the liquid as well, it can not be concluded that a varying current value directly indicates a variation in the oxygen concentration in the liquid. According-ly, in order to determine the variation in the oxygen concentration in the liquid, the liquid 1 must be kept at a constant temperature or the oxygen concentration must be corrected by measuring temper-ature changes so as to be converted into a value at a reference temperature.
With an object to be examined in the form of a living body, however, it is practically difficult to maintain the living body at a constant temperature and such a maintenance is contradictory to the very purpose of on-line monitoring of the living body. For these reasons, appropriate temperature correction is required.
Further, in measuring a partial pressure of gas prevailing in blood with an electrode made of noble metal alone, measured values vary on account of pulsation or lowered reaction ra~e by blood component deposition on the electrode surface. To cope with such problems, it has been proposed to apply various coverings or coatin~s on the electrode surface. Since in this case the covering or coating by itself increases the temperature dependency, the need for temperature correction becomes imminent more and more.
It is an object of the present invention to provide a method and apparatus which provide accurate and rapid measurements o~ a gas partial pressure in a living body on the basis of the polarography principle by compensating for ~emperature changes in the living body.
`:
1 According to the invention, a gas partial pressure sensor having a biomedical electrode disposed in the living body and a separate temperature sensor are disposed in or on the living body, an output value produced from the gas partial pressure sensor is sequentially corrected to a value at a reference temperature by a body temperature information signal produced from the temperature sensor in accord-ance with a predetermined temperature correction formula, and the gas par-tial pressure value is sequentially calculated from a predetermined working curve which represents a relationship between the gas partial pressure value and the corrected output value from the gas partial pressure sensor, both values being at the reference temperature.
The gas partial pressure in the living body is usually represented by a value at a reference temperature of about 37C. But, sometimes the bodily temperature decreases by scores of cen~igrade degrees during a surgical operation. Especially, during an operation for heart disease, the body is sometimes cooled to below 10C. After the operation, the patient often becomes feverish with his temperature rising to over 40C. Thus, the body temperature deviates from the reference temperature by over 10C
during the operation and by 5 to 6C after the operation. Even with the dropping mercury electrode described previously, the electrolytic current varies 1 by 2% as the temperature varies by 1C and with the electrode used in the present invention, the elec-trolytic current varies by about 3%. For example, when the temperature of the living body deviates from the reference temperature by 20C, the electroly-tic current deviates from the actual value by 40 to 60%. Accordingly, without the correction according to the present invention, the measured value of the partial pressure in the living body greatly dif~ers from an actual partial pressure. Conversely, the method of the present invention can reduce the error to a large extent to assure accurate measurement of actual gas partial pressure and can constantly apprize physicians and nurses of an accurate partial pressure in blood or tissue of the patient.
The invention will now be described by way of example with reference to the accompanying drawings.
Fig. 1 is a diagram useful ln explaining the principle of polarography.
Fig. 2 is ano~her diagram similar to Fig. 1 in which a platinum electrode has a porous membrane as a covering.
Fig. 3 is a circuit block diagram of the apparatus according to the present invention.
Fig. 4 is a detailed block diagram of the apparatus according to the present invention.
Referring to Fig. 1, when a voltage is 1 applied across a cathod, or platin~m electrode 1 which constitutes a biomedical electrode) and an anode, or c~unter electrode 2 in a solu~ion 5 in a container 4 from a power supply 3, a polarographic current I
due to electrolytic reaction flows and indicat~d by a meter 6. With a dropping mercury electrode, for example, the current I varies under ~he influence of a temperature dep0ndency because of the gas diffusion by about 2% at l~G. In other words, the amount of the tempexature dependency is about 2%/C.
It has been proposed as shown in Fig. 2 to use a biomedical electrode 1' whose tip end surface is covered with ~ membrane which has an outer thin, dense layer having fine pores of an average diameter of 20 A to 0.7 ~m and an inner porous layer, contiguous to and integral with the outer layer, having fine pores of an average diameter of 0.7 ~m or more, in European patent application No. 122,952 published on October 31st, 1984 assigned to the same assignee as the present applica-tion. When the electrode 1' having its tip end covered with such a porou.s membrane 7 is used, the temperature dependency is larger than that of the dropping mercury elec~rode since many fac~ors such as pore size, surface affinity and thickness of the porous membrane 7 affect the gas diffusion to increase the temperature dependency, there~y adversely a~fecting the gas partial pressure mea~urement.
1 Through various experiments, the present inventors found that the polarographic current I was in a certain relationship with temperatures of an object to be examined, and that temperature correction for the polarographic current can be accomplished by using this relationship.
Fig. 3 is a block diagram for implementation of the present invention. In this embodiment, in order to eliminate the problems described previously, an operation unit 12 comprise a microcomputer board so that a value of a polarographic current I from a gas partial pre~sure sensor 8 is digitized by an interface unit 9 and inputted to the operation unit 12 and a biomedical temperature information signal from a temperature sensor 10 is digitized by the interface unit 9 and inputted to the operation unit 12, and the polarographic current value is temper-ature corrected and calculated from a predetermined value and a programmed formula to provide a gas partial pressure value. The same results may be obtained by an operation unit 12 of a mini-computer or of a dedicated logical circuit which substitutes ~or the microcomputer operation unit 12. A subsidiary advantage of the present invention resides in that in addition to the input data for a working curve preparation which is programmed in advance of measurement, a new value can be readily inputted at a desired time.
1 More particularly, in accordance with the principle of polarography, the output signal of the partial pressure sensor developing in the course of time lapse varies with not only temperatures but also, for example, amounts of substance in blood component deposited to the platinum electrode, or biomedical electrode as described previously, and variations in the sensor output signal can not be eliminated completely even when the porous membrane 7 is applied to the platinum electrode 1'. Accord-ingly, it is very advantageous that a new value ~or the working curve preparation can be inputted in an advanced phase of the measurement.
Correction procedures will now be described specifically.
As far as the principle of polarography is concerned, the linear relation, as represented by P = aI ~ b ............ (1) where P is the gas partial pressure, I is the polarographic current value and a and b are constants, is held between the polarographic current value I
and the gas partial pressure P. Accordingly, the gas partial pressure can be detexmined by measuring the polarographic cuxrent value whenever constants a and b are determined in advance. In this manner, a working current which represents the equation ~1) 1 can be prepared.
The polarographic current I has a temperature dependency as described previously. The present inventors have studied the temperature dependency in various ~anners to find that the following equation holds approximately between the current value and the temperature:
T-t It = ISTk ................... ~23 where It is the polarographic current value at a temperature t, IST is the polarographic value at a reference temperature T, and k is the temperature co-efficient. The temperature coefficient k is specific to the gas partial pressure sensor but sensors produced under the same condition have substantially the same temperature coefficient. For precise measurement, however, several kinds of solutions having known gas partial pressure values are prepared, and currents and temperatures are measured for the respective kinas of solutions at different temper~
atures so that a temperature coefficient specific to a sensor participating in the measurement can be obtained through a method of least squares. For example, gas partial pressure sensors with platinum electrodes covered with the aforementioned porous membrane which were produced in the same lot under a certain condition had an average temperature _ 9 _ l coefficien-t k of 0.97 with a variation of +0.01.
It follows therefore that if the manufacture condition is monitored and controlled properly, the temper-ature coefficient will satisfactorily be regarded as being constant for the purpose of the measurement.
Next, the relationship between gas partial pressure and current value, i.e., the working curve can be determined in various manners as itemized below.
(i) A biomedical electrode of a gas partial pressure sensor is placed in an object to be measured and a temperature sensor is placed in or on the object to be measured. The working curve is then determined from a current value I and a temperature t at this time, and a gas partial pressure value PST
in the object measured by using a separate instrument such as for example a commercially available batch type oxygen partial pressure measuring instrument, and a residual current value Io ~current value for a gas partial pressure of zero) of the gas partial press~re sensor as well. More particularly, a current value I5T at a reference temperature TC is determined from a current value It measured at a temperature tC in accordance with equation (2).
The corrected current value IST thus determined is related to the gas partial pressure PST
in accordance with the equation (l). The constant a in the equation (1) representative of a gradient of -- 10 _ 1 the current value relative to the gas partial PST/(IsT- Io)~ and the constant b which is P -aI is determined by substituting PST
PST/(IST- Io)~ and IST for P, a and I, respectively, in equation (1).
(ii~ A gas partial pressure sensor and a temper-ature measuring sensor are placed in one kind of solution having a known gas partial pressure value PST.
The working curve is then determined from current value I, temperature t, gas partial pressure value P and residual current Io of the gas partial pressure sensor at this condition. Subsequently, constants a and b are determined as in item (i).
(iii) A gas partial pressure sensor and a temperature sensor are placed in two kinds of solutions having kno~n gas partial pressure values, and the working curve is determined from current values I
and I2, temperatures t1 and t2, and gas partia].
pressure values Pl and P2 for the different kinds of solutions in those conditions. In particular, the current value I1 for one solution and the current value I2 for the other solution are respectively converted into current values at the reference temper-ature TC as in items (i) and (ii) and the constants _ and b in the equation (1) are determined in a similar manner.
The gas partial pressure P can be determined from the polarographic current value I in accordance 1 with the equation (1), by using the constants a and b obtained through any one of methods itemized in (i), (iil and (iii) as above.
The residual current Io in (i) and (ii) 5 methods is specific to the gas partial pressure sensor. However, the present inventors have found that sensors produced under the same condition have substantially the same value of residual current in terms of a converted gas partial pressure, as well as the temperature coefficient k. For example, gas partial pressure sensors with platinum electrodes ~biomedical electrodes) co~ered with the aforementioned prorous membrane have a residual current which corresponds to an oxygen gas partial pressure of about 2 mmHg. As a result, the value of 2 mmHg may be used as the value of b instead of measuring the residual current Io~ The solution having a known gas partial pressure is required to contain electrolytic ions and preferably, it is physiological saline solution or blood.
The operator can select at will one of the above three methods. A fixed value of the temperature coefficient k is inputted in advance but for precise measurement, the operator may input a value of the temperature coef~icient k specified to a sensor used.
An arrangement for implementing the present invention will now be described with reference to l Fig. 4.
In Fig. ~, an output signal from a gas partial pressure sensor 8 is arnplified suitably by a partial pressure amplifier 25 and transferred to a CPU bus line 34 ~ia a remote switch 21 and an A/D converter 20. Connected to the CPU bus line 34 are a CPU 13, a ROM 14, a RAM 15, a timer 16 and various I/O and O/P boards 17, 22, 23 and 24.
The output signal from the gas partial pressure sensor 8 transferred to the CPU bus 34 is stored in a pertinent area of the RAM 15 in accordance with a program which has been stored in the ROM 14 and by the action of the CPU 13. On the other hand, the output signal from the temperature sensor 10 is transferred to the CPU 13 via a temperature amplifier 26, the remote switch 21, the A/D converter 20 and CPU bus 34. The remote switch 21 is changed over at a suitable interval by a command via an O/P 22 in accordance with the program, for example, an interval of 200 to 1000 msec in a measurement of a partial pressure of oxygen in blood, and an interval of 5 to 10 sec in a measurement of a partial pres-sure in tissue in a living body, since in these cases generally, there is no need to recognize extremely rapid changes in the partial pressure and temperature. As a result, the output signals from the gas partial pressure sensor 8 and temperature sensor 10 are alternately and intermittently 1 transferred to the CPU 13 depending on the int2rval of the change over of the remote switch 21.
In the CPU 13, a polarographic current value It represented by the output signal of the gas partial pressure sensor 8 is corrected to a current value IsT at a reference temperature T as described in the foregoing, and then a gas partial pressure P
at the reference temperature T is calculated on the basis of the corrected current value IST and the working curve. The resultant gas partial pressure P
calculated sequentially, or rather practically almost continuously, is displayed on a display 27 numerically and is recorded graphically and numerical-ly on a graphic printer 18, together with the temper-ature value or the gas partial pressure alone.
An extension line may also be provided fordelivery of the data to an ordinary analog recorder 30 where the recorder 30 is additionally used.
A console panel 28 includes a plurality of console panel switches adapted to input data for preparing the working curve before or during the calculation of the gas partial pressure and to input ~he operator instructions in order to effect an accurate and updated correction. The console panel 28 further includes pilot lamps (not shown) adapted to confirm or monitor the operations in progress.
The one series of data input system illustrated herein may be modified by doubling blocks 1 marked with a symbol * in Fig. 4 (gas partial pressure sensor 8, temperature measuring sensor 10, remote switch 21, A/D converter, etc.). With this modifica-tion, oxygen partial pressure in arterial blood and that in a biological tissue inside the myocardium can be measured simultaneously. Results of the simultaneous measurements may be recorded on a single recorder 30 or graphic printer 18. If the results of the measurements exceed a predetermined control limit, audible or visual alarm unit 29 may be energized.
For protection of the living body, especially, in the measurement of a human body, the amplifiers 25 and 26 may respectively include isolation amplifier circuits employing a transformer coupling. Further, other lines may also be isolated electrically.
As has been described, the method of the present invention permits accurate and continuous monitoring of gas partial pressure in the living body and can be applied advantageously to clinical and experimental medical treatment.
In particular, the present invention relates to a method and apparatus for measuring a partial pressure in a living body on the basis of the polarography.
Two major methods have hitherto been available for the gas concentration or partial pres-sure measurements.
A first method is based on the so-called spot measurement wherein blood is extracted from a living body and thereafter the gas concentration is measured through a chemical analysis process.
A second method is such that a small sensor is inserted into a living body and the gas concentration in terms of an electrical signal is measured.
Disadvantageously, the ~irst method consumes much time until a result is obtained and besides, blood being in process of extraction from the living bod~ tends to come into contact with ambient air, and gas exchan~e will be caused between the extracted blood and the ambient aix with the result l that a measured value representative of a gas concentration in the llving body tends to be inaccurate.
The second method, on the other hand, is excellent which can provide instantaneous results, thus facilitating on-line monitoring of a living body condition. This method has therefore been proposed in various ways mainly on the basis of polarographic process. This method utilizing an electrode reaction, however, faces a problem of temperature dependency in which current or voltage values obtained greatly depend on temperatures and accurate values can not be obtained unless the living body is kept at a constant temperature.
For example, a sensor for measuring the oxygen concentration in liquid usually has a cathode made of a noble metal such as gold, platinum, silver or the like and an anode, such as an Ag/AgCl electrode.
Oxygen is reduced at the surface of the cathode and a polarographic current which has a small current value due to the reducing reaction is measured.
Since the current value varies with an oxygen concentration in the liquid and temperature of the liquid as well, it can not be concluded that a varying current value directly indicates a variation in the oxygen concentration in the liquid. According-ly, in order to determine the variation in the oxygen concentration in the liquid, the liquid 1 must be kept at a constant temperature or the oxygen concentration must be corrected by measuring temper-ature changes so as to be converted into a value at a reference temperature.
With an object to be examined in the form of a living body, however, it is practically difficult to maintain the living body at a constant temperature and such a maintenance is contradictory to the very purpose of on-line monitoring of the living body. For these reasons, appropriate temperature correction is required.
Further, in measuring a partial pressure of gas prevailing in blood with an electrode made of noble metal alone, measured values vary on account of pulsation or lowered reaction ra~e by blood component deposition on the electrode surface. To cope with such problems, it has been proposed to apply various coverings or coatin~s on the electrode surface. Since in this case the covering or coating by itself increases the temperature dependency, the need for temperature correction becomes imminent more and more.
It is an object of the present invention to provide a method and apparatus which provide accurate and rapid measurements o~ a gas partial pressure in a living body on the basis of the polarography principle by compensating for ~emperature changes in the living body.
`:
1 According to the invention, a gas partial pressure sensor having a biomedical electrode disposed in the living body and a separate temperature sensor are disposed in or on the living body, an output value produced from the gas partial pressure sensor is sequentially corrected to a value at a reference temperature by a body temperature information signal produced from the temperature sensor in accord-ance with a predetermined temperature correction formula, and the gas par-tial pressure value is sequentially calculated from a predetermined working curve which represents a relationship between the gas partial pressure value and the corrected output value from the gas partial pressure sensor, both values being at the reference temperature.
The gas partial pressure in the living body is usually represented by a value at a reference temperature of about 37C. But, sometimes the bodily temperature decreases by scores of cen~igrade degrees during a surgical operation. Especially, during an operation for heart disease, the body is sometimes cooled to below 10C. After the operation, the patient often becomes feverish with his temperature rising to over 40C. Thus, the body temperature deviates from the reference temperature by over 10C
during the operation and by 5 to 6C after the operation. Even with the dropping mercury electrode described previously, the electrolytic current varies 1 by 2% as the temperature varies by 1C and with the electrode used in the present invention, the elec-trolytic current varies by about 3%. For example, when the temperature of the living body deviates from the reference temperature by 20C, the electroly-tic current deviates from the actual value by 40 to 60%. Accordingly, without the correction according to the present invention, the measured value of the partial pressure in the living body greatly dif~ers from an actual partial pressure. Conversely, the method of the present invention can reduce the error to a large extent to assure accurate measurement of actual gas partial pressure and can constantly apprize physicians and nurses of an accurate partial pressure in blood or tissue of the patient.
The invention will now be described by way of example with reference to the accompanying drawings.
Fig. 1 is a diagram useful ln explaining the principle of polarography.
Fig. 2 is ano~her diagram similar to Fig. 1 in which a platinum electrode has a porous membrane as a covering.
Fig. 3 is a circuit block diagram of the apparatus according to the present invention.
Fig. 4 is a detailed block diagram of the apparatus according to the present invention.
Referring to Fig. 1, when a voltage is 1 applied across a cathod, or platin~m electrode 1 which constitutes a biomedical electrode) and an anode, or c~unter electrode 2 in a solu~ion 5 in a container 4 from a power supply 3, a polarographic current I
due to electrolytic reaction flows and indicat~d by a meter 6. With a dropping mercury electrode, for example, the current I varies under ~he influence of a temperature dep0ndency because of the gas diffusion by about 2% at l~G. In other words, the amount of the tempexature dependency is about 2%/C.
It has been proposed as shown in Fig. 2 to use a biomedical electrode 1' whose tip end surface is covered with ~ membrane which has an outer thin, dense layer having fine pores of an average diameter of 20 A to 0.7 ~m and an inner porous layer, contiguous to and integral with the outer layer, having fine pores of an average diameter of 0.7 ~m or more, in European patent application No. 122,952 published on October 31st, 1984 assigned to the same assignee as the present applica-tion. When the electrode 1' having its tip end covered with such a porou.s membrane 7 is used, the temperature dependency is larger than that of the dropping mercury elec~rode since many fac~ors such as pore size, surface affinity and thickness of the porous membrane 7 affect the gas diffusion to increase the temperature dependency, there~y adversely a~fecting the gas partial pressure mea~urement.
1 Through various experiments, the present inventors found that the polarographic current I was in a certain relationship with temperatures of an object to be examined, and that temperature correction for the polarographic current can be accomplished by using this relationship.
Fig. 3 is a block diagram for implementation of the present invention. In this embodiment, in order to eliminate the problems described previously, an operation unit 12 comprise a microcomputer board so that a value of a polarographic current I from a gas partial pre~sure sensor 8 is digitized by an interface unit 9 and inputted to the operation unit 12 and a biomedical temperature information signal from a temperature sensor 10 is digitized by the interface unit 9 and inputted to the operation unit 12, and the polarographic current value is temper-ature corrected and calculated from a predetermined value and a programmed formula to provide a gas partial pressure value. The same results may be obtained by an operation unit 12 of a mini-computer or of a dedicated logical circuit which substitutes ~or the microcomputer operation unit 12. A subsidiary advantage of the present invention resides in that in addition to the input data for a working curve preparation which is programmed in advance of measurement, a new value can be readily inputted at a desired time.
1 More particularly, in accordance with the principle of polarography, the output signal of the partial pressure sensor developing in the course of time lapse varies with not only temperatures but also, for example, amounts of substance in blood component deposited to the platinum electrode, or biomedical electrode as described previously, and variations in the sensor output signal can not be eliminated completely even when the porous membrane 7 is applied to the platinum electrode 1'. Accord-ingly, it is very advantageous that a new value ~or the working curve preparation can be inputted in an advanced phase of the measurement.
Correction procedures will now be described specifically.
As far as the principle of polarography is concerned, the linear relation, as represented by P = aI ~ b ............ (1) where P is the gas partial pressure, I is the polarographic current value and a and b are constants, is held between the polarographic current value I
and the gas partial pressure P. Accordingly, the gas partial pressure can be detexmined by measuring the polarographic cuxrent value whenever constants a and b are determined in advance. In this manner, a working current which represents the equation ~1) 1 can be prepared.
The polarographic current I has a temperature dependency as described previously. The present inventors have studied the temperature dependency in various ~anners to find that the following equation holds approximately between the current value and the temperature:
T-t It = ISTk ................... ~23 where It is the polarographic current value at a temperature t, IST is the polarographic value at a reference temperature T, and k is the temperature co-efficient. The temperature coefficient k is specific to the gas partial pressure sensor but sensors produced under the same condition have substantially the same temperature coefficient. For precise measurement, however, several kinds of solutions having known gas partial pressure values are prepared, and currents and temperatures are measured for the respective kinas of solutions at different temper~
atures so that a temperature coefficient specific to a sensor participating in the measurement can be obtained through a method of least squares. For example, gas partial pressure sensors with platinum electrodes covered with the aforementioned porous membrane which were produced in the same lot under a certain condition had an average temperature _ 9 _ l coefficien-t k of 0.97 with a variation of +0.01.
It follows therefore that if the manufacture condition is monitored and controlled properly, the temper-ature coefficient will satisfactorily be regarded as being constant for the purpose of the measurement.
Next, the relationship between gas partial pressure and current value, i.e., the working curve can be determined in various manners as itemized below.
(i) A biomedical electrode of a gas partial pressure sensor is placed in an object to be measured and a temperature sensor is placed in or on the object to be measured. The working curve is then determined from a current value I and a temperature t at this time, and a gas partial pressure value PST
in the object measured by using a separate instrument such as for example a commercially available batch type oxygen partial pressure measuring instrument, and a residual current value Io ~current value for a gas partial pressure of zero) of the gas partial press~re sensor as well. More particularly, a current value I5T at a reference temperature TC is determined from a current value It measured at a temperature tC in accordance with equation (2).
The corrected current value IST thus determined is related to the gas partial pressure PST
in accordance with the equation (l). The constant a in the equation (1) representative of a gradient of -- 10 _ 1 the current value relative to the gas partial PST/(IsT- Io)~ and the constant b which is P -aI is determined by substituting PST
PST/(IST- Io)~ and IST for P, a and I, respectively, in equation (1).
(ii~ A gas partial pressure sensor and a temper-ature measuring sensor are placed in one kind of solution having a known gas partial pressure value PST.
The working curve is then determined from current value I, temperature t, gas partial pressure value P and residual current Io of the gas partial pressure sensor at this condition. Subsequently, constants a and b are determined as in item (i).
(iii) A gas partial pressure sensor and a temperature sensor are placed in two kinds of solutions having kno~n gas partial pressure values, and the working curve is determined from current values I
and I2, temperatures t1 and t2, and gas partia].
pressure values Pl and P2 for the different kinds of solutions in those conditions. In particular, the current value I1 for one solution and the current value I2 for the other solution are respectively converted into current values at the reference temper-ature TC as in items (i) and (ii) and the constants _ and b in the equation (1) are determined in a similar manner.
The gas partial pressure P can be determined from the polarographic current value I in accordance 1 with the equation (1), by using the constants a and b obtained through any one of methods itemized in (i), (iil and (iii) as above.
The residual current Io in (i) and (ii) 5 methods is specific to the gas partial pressure sensor. However, the present inventors have found that sensors produced under the same condition have substantially the same value of residual current in terms of a converted gas partial pressure, as well as the temperature coefficient k. For example, gas partial pressure sensors with platinum electrodes ~biomedical electrodes) co~ered with the aforementioned prorous membrane have a residual current which corresponds to an oxygen gas partial pressure of about 2 mmHg. As a result, the value of 2 mmHg may be used as the value of b instead of measuring the residual current Io~ The solution having a known gas partial pressure is required to contain electrolytic ions and preferably, it is physiological saline solution or blood.
The operator can select at will one of the above three methods. A fixed value of the temperature coefficient k is inputted in advance but for precise measurement, the operator may input a value of the temperature coef~icient k specified to a sensor used.
An arrangement for implementing the present invention will now be described with reference to l Fig. 4.
In Fig. ~, an output signal from a gas partial pressure sensor 8 is arnplified suitably by a partial pressure amplifier 25 and transferred to a CPU bus line 34 ~ia a remote switch 21 and an A/D converter 20. Connected to the CPU bus line 34 are a CPU 13, a ROM 14, a RAM 15, a timer 16 and various I/O and O/P boards 17, 22, 23 and 24.
The output signal from the gas partial pressure sensor 8 transferred to the CPU bus 34 is stored in a pertinent area of the RAM 15 in accordance with a program which has been stored in the ROM 14 and by the action of the CPU 13. On the other hand, the output signal from the temperature sensor 10 is transferred to the CPU 13 via a temperature amplifier 26, the remote switch 21, the A/D converter 20 and CPU bus 34. The remote switch 21 is changed over at a suitable interval by a command via an O/P 22 in accordance with the program, for example, an interval of 200 to 1000 msec in a measurement of a partial pressure of oxygen in blood, and an interval of 5 to 10 sec in a measurement of a partial pres-sure in tissue in a living body, since in these cases generally, there is no need to recognize extremely rapid changes in the partial pressure and temperature. As a result, the output signals from the gas partial pressure sensor 8 and temperature sensor 10 are alternately and intermittently 1 transferred to the CPU 13 depending on the int2rval of the change over of the remote switch 21.
In the CPU 13, a polarographic current value It represented by the output signal of the gas partial pressure sensor 8 is corrected to a current value IsT at a reference temperature T as described in the foregoing, and then a gas partial pressure P
at the reference temperature T is calculated on the basis of the corrected current value IST and the working curve. The resultant gas partial pressure P
calculated sequentially, or rather practically almost continuously, is displayed on a display 27 numerically and is recorded graphically and numerical-ly on a graphic printer 18, together with the temper-ature value or the gas partial pressure alone.
An extension line may also be provided fordelivery of the data to an ordinary analog recorder 30 where the recorder 30 is additionally used.
A console panel 28 includes a plurality of console panel switches adapted to input data for preparing the working curve before or during the calculation of the gas partial pressure and to input ~he operator instructions in order to effect an accurate and updated correction. The console panel 28 further includes pilot lamps (not shown) adapted to confirm or monitor the operations in progress.
The one series of data input system illustrated herein may be modified by doubling blocks 1 marked with a symbol * in Fig. 4 (gas partial pressure sensor 8, temperature measuring sensor 10, remote switch 21, A/D converter, etc.). With this modifica-tion, oxygen partial pressure in arterial blood and that in a biological tissue inside the myocardium can be measured simultaneously. Results of the simultaneous measurements may be recorded on a single recorder 30 or graphic printer 18. If the results of the measurements exceed a predetermined control limit, audible or visual alarm unit 29 may be energized.
For protection of the living body, especially, in the measurement of a human body, the amplifiers 25 and 26 may respectively include isolation amplifier circuits employing a transformer coupling. Further, other lines may also be isolated electrically.
As has been described, the method of the present invention permits accurate and continuous monitoring of gas partial pressure in the living body and can be applied advantageously to clinical and experimental medical treatment.
Claims (16)
1. A method of measuring a partial pressure of gas in a living body on the basis of the principle of polarography, comprising the steps of:
placing a biomedical electrode of a gas partial pressure sensor in said living body to sequentially produce an output signal from said gas partial pressure sensor, placing a temperature sensor at a position suitable to sequentially measure a temperature of said living body, continuously correcting a value of the output signal from said gas partial pressure sensor to a value at a reference temperature on the basis of the temperature measured by said temperature sensor, and continuously determining a gas partial pressure value of said living body from said corrected output value and a working curve, said working curve being prepared in advance and representing a relationship between the gas partial pressure value and said corrected output value.
placing a biomedical electrode of a gas partial pressure sensor in said living body to sequentially produce an output signal from said gas partial pressure sensor, placing a temperature sensor at a position suitable to sequentially measure a temperature of said living body, continuously correcting a value of the output signal from said gas partial pressure sensor to a value at a reference temperature on the basis of the temperature measured by said temperature sensor, and continuously determining a gas partial pressure value of said living body from said corrected output value and a working curve, said working curve being prepared in advance and representing a relationship between the gas partial pressure value and said corrected output value.
2. A method of measuring a gas partial pressure according to Claim 1 wherein the output signal of said gas partial pressure sensor is corrected to the output value at the reference temperature in accordance with the following equation:
It = IsT (k)T-t where It is the output value of the gas partial pressure sensor at t°C, IsT is the output value of the gas partial pressure sensor at T°C, k is the temperature coefficient of the gas partial pressure sensor, and t is the temperature measured by the temperature sensor.
It = IsT (k)T-t where It is the output value of the gas partial pressure sensor at t°C, IsT is the output value of the gas partial pressure sensor at T°C, k is the temperature coefficient of the gas partial pressure sensor, and t is the temperature measured by the temperature sensor.
3. A method of measuring a gas partial pressure according to Claim 1 wherein the working curve is determined by making the output value of said gas partial pressure sensor related to a gas partial pressure value which is measured in advance.
4. A method of measuring a gas partial pressure according to Claim 1 wherein the working curve is determined by placing said gas partial pressure sensor in one kind of test solution having a known gas partial pressure value and by using an output value and a residual current value of said gas partial pressure sensor and the known gas partial pressure value of the test solution.
5. A method of measuring a gas partial pres-sure according to Claim 1 wherein the working curve is determined by placing said gas partial pressure sensor in two kinds of test solutions having known gas partial pressure values and by using output values of said gas partial pressure sensor and the corresponding known gas partial pressure values of the test solutions.
6. A method of measuring a gas partial pressure according to Claim 1 wherein said gas partial pressure sensor comprises a biomedical electrode whose tip end surface is covered with a membrane comprised of an outer dense, thin layer having fine pores of an average diameter of 20 .ANG.
to 0.7 µm and an inner porous layer, contiguous to and integral with the outer layer, having fine pores of an average diameter of 0.7 µm or more.
to 0.7 µm and an inner porous layer, contiguous to and integral with the outer layer, having fine pores of an average diameter of 0.7 µm or more.
7. A method of measuring a gas partial pressure according to Claim 4 wherein said test solu-tion is physiological saline solution.
8. A method of measuring a gas partial pressure according to Claim 4 wherein said test solution is blood.
9. A method of measuring a gas partial pressure according to Claim 5 wherein said two kinds of test solutions are selected from a group of physiolog-ical saline solutions each having a different gas pressure and a group of bloods each having a different gas partial pressure.
10. An apparatus for measuring a partial pressure of gas in a living body based on the principle of polarography comprising:
a gas partial pressure sensor including a biomedical electrode and a counter electrode for producing an output signal indicative of a polaro-graphic current, at least said biomedical electrode being placed in said living body, a temperature sensor placed at a position suitable for producing an output signal indicative of a temperature of said living body, an interface unit having an A/D converter which digitizes the output signals from said partial pressure sensor and said temperature sensor, an operation unit connected to said interface unit to receive the digitized values of the output signals of said gas partial pressure sensor and said temperature sensor, said operation unit correcting the digitized output signal of said gas partial pressure sensor to an output signal value at a reference temperature and calculating a gas partial pressure on the basis of a working curve prepared in said operation unit in advance, said working curve representing a relationship between said gas partial pressure and said corrected digitized output signal of said gas partial pressure sensor.
a gas partial pressure sensor including a biomedical electrode and a counter electrode for producing an output signal indicative of a polaro-graphic current, at least said biomedical electrode being placed in said living body, a temperature sensor placed at a position suitable for producing an output signal indicative of a temperature of said living body, an interface unit having an A/D converter which digitizes the output signals from said partial pressure sensor and said temperature sensor, an operation unit connected to said interface unit to receive the digitized values of the output signals of said gas partial pressure sensor and said temperature sensor, said operation unit correcting the digitized output signal of said gas partial pressure sensor to an output signal value at a reference temperature and calculating a gas partial pressure on the basis of a working curve prepared in said operation unit in advance, said working curve representing a relationship between said gas partial pressure and said corrected digitized output signal of said gas partial pressure sensor.
11. An apparatus for measuring a gas partial pressure according to Claim 10 further comprising a display unit for displaying the gas partial pres-sure calculated by said operation unit.
12. An apparatus for measuring a gas partial pressure according to Claim 10 further comprising an alarm unit which is energized when the results of the calculation are beyond a predetermined control limit.
13. An apparatus for measuring a gas partial pressure according to Claim 10 wherein each of said gas partial pressure sensor and temperature sensor is connected to an amplifier having an isolation amplifier circuit in said interface unit.
14. An apparatus for measuring a gas partial pressure according to Claim 10 further comprising.
a graphic printer for recording the gas partial pressure calculated by said operation unit.
a graphic printer for recording the gas partial pressure calculated by said operation unit.
15. An apparatus for measuring a gas partial pressure according to Claim 14 further comprising a second set of a gas partial pressure sensor, a temperature sensor, and an interface unit, whereby to form two sets of gas partial pressure measurement systems, and results of the two sets of measurement systems are simultaneously displayed on said single graphic printer.
16. An apparatus for measuring a gas partial pressure according to Claim 10 wherein said biomedical electrode has a tip end surface covered with a membrane comprised of an outer dense, thin layer having fine pores of an average diameter of 20 .ANG. to 0.7 µm and an inner porous layer, contiguous to and integral with the outer layer, having fine pores of an average diameter of 0.7 µm or more.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA000426165A CA1201169A (en) | 1983-04-19 | 1983-04-19 | Method and apparatus for measuring gas partial pressure in living body |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA000426165A CA1201169A (en) | 1983-04-19 | 1983-04-19 | Method and apparatus for measuring gas partial pressure in living body |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1201169A true CA1201169A (en) | 1986-02-25 |
Family
ID=4125048
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000426165A Expired CA1201169A (en) | 1983-04-19 | 1983-04-19 | Method and apparatus for measuring gas partial pressure in living body |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA1201169A (en) |
-
1983
- 1983-04-19 CA CA000426165A patent/CA1201169A/en not_active Expired
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