CA1187786A - Agent for immunological diagnosis and a process for its preparation - Google Patents
Agent for immunological diagnosis and a process for its preparationInfo
- Publication number
- CA1187786A CA1187786A CA000399130A CA399130A CA1187786A CA 1187786 A CA1187786 A CA 1187786A CA 000399130 A CA000399130 A CA 000399130A CA 399130 A CA399130 A CA 399130A CA 1187786 A CA1187786 A CA 1187786A
- Authority
- CA
- Canada
- Prior art keywords
- carrier
- partner
- irradiated
- gamma radiation
- bound
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K41/00—Medicinal preparations obtained by treating materials with wave energy or particle radiation ; Therapies using these preparations
- A61K41/0023—Agression treatment or altering
Abstract
Abstract of the Disclosure A process for preparing a carrier for use in the detection and determination of a partner of an immunological reaction, the associated partner being on the surface of the carrier, in which the carrier is irradiated with gamma radiation before or after the associated partner is bound thereto. A polymeric carrier produced by the process is also disclosed.
Description
The invention relates to a carrier for the detection and determination of an immunological reaction partner present in a liquid, the associated partner being on the surface of the c,^arrier.
It is known to bind a partner of an immunolo~ical reaction adsorptively or covalently to a carrier in order to isolate the corresponding partner from a solution in or-der to detect this partner or to determine its amount.
However, it is al50 known that test methods based On this principle can have deficiencies.
Thus, Chessum and Denmark describe, in The Lancet (1978), page 161, that the known polystyrene microtiter plates used as the carrier in a process for the deter-mination of antigens or antibodies which is generally called ELISA (Enzyme Linked Immuno Sorbent Assay) can lead to intolerably large variations in the test result.
Bidwell et al. also report, in J. Infect. Disease 136, Supplement, page 27~ (1977), variations in the results of an ELISA test which it was possible to attribute to differences in the microtiter plates used as the carrier.
These wide varia-tions in the test result which were caused by differences in the surface properties of the microtiter plates should not be restricted to the use of these plates, but should also occur with carriers of a different shape made from the same materials.
Accordingly, there was the object of discovering '7'7~
carrier materials which have more uniform surface pro-perties and which, when used, give immunological test results which vary less.
Surprisingly, it has now been Eound that this object can be achieved by irradiating the carrier material, and preferably microtiter plates prepared therefrom, with gamma rays, preferably with cobalt rays, and preferably with 0.1 to 100, in particlular 0.5 to 10, megarads.
The invention thus relates to a process for preparing a polymeric carrier for the detection and determination o~ a partner o an immunological reaction in which the corresponding partner is on the surface of the carrier, wherein the carrier is irradiated, before or after its surface has been coated with this partner.
It is not necessary to coat the carrier immediately after irradiation. The advantageous effect of irradiation is also observed if a period of days or months elapses between irradiation and coating.
In principle, all plastics, but in particular polystyrene and polyvinyl chlcride~ can be used as carrier materials in this process.
Irradiated carriers can advantageously be used in immune test systems which are known per se, for example immune test systems based on an antigen/antibody reaction, for example ELISA systems (Enzyme Linked Immuno Sorbent Assay systems), a bonding partner being bonded to the irradiated 77~
carrier.
Such bonding partners can be, for example, proteins, such as immunoglobulins, antimicrobial anti-bodies, plasma proteins, for example Clq, or antigenic materials, for example allergens or microbial antigens.
The example whi.ch follows illustrates the invention:
EXAMPLE
Preparation of a test kit for the determination of anti-bodies of the IgG class and IgM class against mumps virus in human serum.
1. Bonding of the virus antigen to the carrier (polystyrene microtiter plate) a. irradiated (about 2 mrad~
b. not irradiated 15 2. Washing 3. Application and incubation of serum dilutions 4. Washing 5. Application and incubation of anti-human IgG- or IgM-alkaline phosphatase conjugate 20 6. Washing 7. Application of the substrate solution 8. Stopping of enzymatic splitting by addition of 2N NaOH
9. Photometric measurement at 405 nm ~7~
Table Comparison of the sensitivity of a test with an irradi-ated carrier with that of a test with a non-irradiated carrier:
Detection limit (titer 1:) irradiated carrier non-irradiated carrier Serum IgG IgM ~ IgG I~M
A 20,480 20,480 1,280 2,560 10 3 1,280 160 640 The figures show the advantage of using irradi-ated carriers. The detectability (sensitivity) is significantly increased.
It is known to bind a partner of an immunolo~ical reaction adsorptively or covalently to a carrier in order to isolate the corresponding partner from a solution in or-der to detect this partner or to determine its amount.
However, it is al50 known that test methods based On this principle can have deficiencies.
Thus, Chessum and Denmark describe, in The Lancet (1978), page 161, that the known polystyrene microtiter plates used as the carrier in a process for the deter-mination of antigens or antibodies which is generally called ELISA (Enzyme Linked Immuno Sorbent Assay) can lead to intolerably large variations in the test result.
Bidwell et al. also report, in J. Infect. Disease 136, Supplement, page 27~ (1977), variations in the results of an ELISA test which it was possible to attribute to differences in the microtiter plates used as the carrier.
These wide varia-tions in the test result which were caused by differences in the surface properties of the microtiter plates should not be restricted to the use of these plates, but should also occur with carriers of a different shape made from the same materials.
Accordingly, there was the object of discovering '7'7~
carrier materials which have more uniform surface pro-perties and which, when used, give immunological test results which vary less.
Surprisingly, it has now been Eound that this object can be achieved by irradiating the carrier material, and preferably microtiter plates prepared therefrom, with gamma rays, preferably with cobalt rays, and preferably with 0.1 to 100, in particlular 0.5 to 10, megarads.
The invention thus relates to a process for preparing a polymeric carrier for the detection and determination o~ a partner o an immunological reaction in which the corresponding partner is on the surface of the carrier, wherein the carrier is irradiated, before or after its surface has been coated with this partner.
It is not necessary to coat the carrier immediately after irradiation. The advantageous effect of irradiation is also observed if a period of days or months elapses between irradiation and coating.
In principle, all plastics, but in particular polystyrene and polyvinyl chlcride~ can be used as carrier materials in this process.
Irradiated carriers can advantageously be used in immune test systems which are known per se, for example immune test systems based on an antigen/antibody reaction, for example ELISA systems (Enzyme Linked Immuno Sorbent Assay systems), a bonding partner being bonded to the irradiated 77~
carrier.
Such bonding partners can be, for example, proteins, such as immunoglobulins, antimicrobial anti-bodies, plasma proteins, for example Clq, or antigenic materials, for example allergens or microbial antigens.
The example whi.ch follows illustrates the invention:
EXAMPLE
Preparation of a test kit for the determination of anti-bodies of the IgG class and IgM class against mumps virus in human serum.
1. Bonding of the virus antigen to the carrier (polystyrene microtiter plate) a. irradiated (about 2 mrad~
b. not irradiated 15 2. Washing 3. Application and incubation of serum dilutions 4. Washing 5. Application and incubation of anti-human IgG- or IgM-alkaline phosphatase conjugate 20 6. Washing 7. Application of the substrate solution 8. Stopping of enzymatic splitting by addition of 2N NaOH
9. Photometric measurement at 405 nm ~7~
Table Comparison of the sensitivity of a test with an irradi-ated carrier with that of a test with a non-irradiated carrier:
Detection limit (titer 1:) irradiated carrier non-irradiated carrier Serum IgG IgM ~ IgG I~M
A 20,480 20,480 1,280 2,560 10 3 1,280 160 640 The figures show the advantage of using irradi-ated carriers. The detectability (sensitivity) is significantly increased.
Claims (5)
PROPERTY OR PRIVILEGE IS CLAIMED ARE DEFINED AS FOLLOWS:
1. A process for preparing a polymeric carrier for use in the detection and determination of an immunological reaction partner present in a liquid contacted with the carrier, the carrier having the associated partner bound thereto, which process comprises irradiating the carrier with gamma radiation before or after the associated partner is bound to the carrier.
2. A process as claimed in claim 1 wherein the carrier is irradiated with from 0.1 to 100 mégarads of gamma radiation.
3. A process as claimed in claim 2 wherein the carrier is irradiated with from 0.5 to 10 megarads of gamma radiation.
4. A process as claimed in claim 1 wherein said partner bound to the carrier is an antigen or antibody.
5. A process as claimed in claim 1 wherein said polymeric carrier is of polystyrene or polyvinyl chloride.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19813111474 DE3111474A1 (en) | 1981-03-24 | 1981-03-24 | "MEANS FOR IMMUNOLOGICAL DIAGNOSIS AND METHOD FOR THE PRODUCTION THEREOF" |
| DEP3111474.1 | 1981-03-24 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1187786A true CA1187786A (en) | 1985-05-28 |
Family
ID=6128118
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000399130A Expired CA1187786A (en) | 1981-03-24 | 1982-03-23 | Agent for immunological diagnosis and a process for its preparation |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP0061167B1 (en) |
| JP (1) | JPS57169675A (en) |
| AT (1) | ATE14353T1 (en) |
| AU (1) | AU553464B2 (en) |
| CA (1) | CA1187786A (en) |
| DE (2) | DE3111474A1 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5378638A (en) * | 1990-08-02 | 1995-01-03 | Boehringer Mannheim Gmbh | Analysis element and process for its manufacture |
| US5424220A (en) * | 1992-01-31 | 1995-06-13 | Boehringer Mannheim Gmbh | Analysis element and method for determination of an analyte in a liquid sample |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60260857A (en) * | 1984-06-08 | 1985-12-24 | Sumitomo Bakelite Co Ltd | Molding for fixed phase immunomeasurement method |
| WO1987001120A1 (en) * | 1985-08-19 | 1987-02-26 | Royal Melbourne Institute Of Technology Limited | Method of attaching biological substances to solid substrates |
| AU605746B2 (en) * | 1985-08-19 | 1991-01-24 | Royal Melbourne Institute Of Technology Limited | Method of attaching biological substances to solid substrates |
| DE3717401A1 (en) | 1987-05-23 | 1988-12-08 | Behringwerke Ag | One-step immunoassay for the determination of antigen-specific antibodies from one of the immunoglobulin classes A, M, D or E and a suitable agent |
| FR2629916B1 (en) * | 1988-04-08 | 1992-09-04 | Hoechst Behring Applic Pharmac | SOLID SUPPORT FOR IMMUNOLOGICAL DIAGNOSIS, PROCESS FOR THEIR TREATMENT AND APPLICATION TO IMMUNOLOGICAL DIAGNOSIS |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1238280A (en) * | 1968-11-06 | 1971-07-07 |
-
1981
- 1981-03-24 DE DE19813111474 patent/DE3111474A1/en not_active Withdrawn
-
1982
- 1982-03-19 AT AT82102257T patent/ATE14353T1/en not_active IP Right Cessation
- 1982-03-19 DE DE8282102257T patent/DE3264726D1/en not_active Expired
- 1982-03-19 EP EP82102257A patent/EP0061167B1/en not_active Expired
- 1982-03-23 CA CA000399130A patent/CA1187786A/en not_active Expired
- 1982-03-23 JP JP57044778A patent/JPS57169675A/en active Pending
- 1982-03-23 AU AU81818/82A patent/AU553464B2/en not_active Ceased
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5378638A (en) * | 1990-08-02 | 1995-01-03 | Boehringer Mannheim Gmbh | Analysis element and process for its manufacture |
| US5424220A (en) * | 1992-01-31 | 1995-06-13 | Boehringer Mannheim Gmbh | Analysis element and method for determination of an analyte in a liquid sample |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS57169675A (en) | 1982-10-19 |
| ATE14353T1 (en) | 1985-08-15 |
| DE3111474A1 (en) | 1982-10-07 |
| AU553464B2 (en) | 1986-07-17 |
| DE3264726D1 (en) | 1985-08-22 |
| EP0061167A1 (en) | 1982-09-29 |
| EP0061167B1 (en) | 1985-07-17 |
| AU8181882A (en) | 1982-09-30 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKEX | Expiry |