CA1185153A - Agent for detecting glucose in biological fluids - Google Patents
Agent for detecting glucose in biological fluidsInfo
- Publication number
- CA1185153A CA1185153A CA000414392A CA414392A CA1185153A CA 1185153 A CA1185153 A CA 1185153A CA 000414392 A CA000414392 A CA 000414392A CA 414392 A CA414392 A CA 414392A CA 1185153 A CA1185153 A CA 1185153A
- Authority
- CA
- Canada
- Prior art keywords
- agent
- nitrate
- absorber
- glucose
- chromogen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
Abstract
Abstract of the disclosure:
An agent for detecting glucose in biological fluids is described, which is essentially composed of an adsorbent matrix, a glucose oxidase, a peroxidase and a chromogen. This agent contains a nitrate and can contain a UV absorber.
An agent for detecting glucose in biological fluids is described, which is essentially composed of an adsorbent matrix, a glucose oxidase, a peroxidase and a chromogen. This agent contains a nitrate and can contain a UV absorber.
Description
L r
- 2 ~OE ~1/8 012 The inver,tion relates to an ac3ent for detecc;ng glucose in biological fluids, ~hich essentially com~
prises an adsorbent matrix~ a glucose oxidase, a peroxid-ase and a chromogen as re3gent anci contains a nitrate and, optionally~ a UV absorber.
Diabetes mellitus is a chronic disease ~hich must be continuously monitored. It has proved to be reason~
able for the diabetic to monitor his o~ln metabolic status afte,r ins~ruct;on by ~he phys;cian ;n order to be able, 1~ if necessary, immediately to adjust the necessary insulir dose ~o suit ~he changing requiremcnts. The check of metaboLism is carried out sush that the conten~ of ~lucose in the urine or in the blood is routineLy determined. In general, the diabetic determines his O~il urinary glucose value semiquantitatively, whilst~ in contrast, quantitative measuremen~ of blood sugar is carried out by an expert.
The determination of urinary glucose sl1ould cover a concentration rancge up to about 5 g of c3lucose/100 ml ~- ~f urine.
hi present, there arè t,to met,hods for rapid semi--~uantitative C~etermiQatiOn o-F c3lucose:
1. Test ;ablecs accordincJ to the non-specific reduction r,letllocl o-F ~ EDlKr and ~ Test strips ~hich are irDprec~nated ~ich c~lucoce oxidase, ' ' 3 which reacts soecifically, a chromogen and a peroxidase.
The glucose oxidase in the test strips catalyzes the ox;dation of glucose with oxygen to for~, gLuconolact-one and hydrogen peroxide. In a subsequent non-speçifi~
reaction~ ehe latter oxidizes a chromogen, ~i~h peroxid-ase catalysis, to g;ve a dyestuff.
The t~o methods ~entioned are associated uith dis-ad~antages: -! Th2 reduction method is non-specific, since other reducing com?ounds, such as, for exanple, galactose, are also de~ected. Furtherrrlore, i~ is necessary to use corrosive reagents for this rnethod (G. Friedrich~ "Der ;nformierte Arzt" 8 (9), Off-print, 1~80). In addition, ~he reaction is associa~ed ~ith a not inconsiderable 'l~ evolution ~f heat alld ~iih an interle~ g forr,7atiun of foam. The performance of ~he tests ~lith tablets and ~est tubes is elaborate, inconvenient and costly.
The same applies to the second rnethod of deterrnin-at;on~ S;nce thc semiquantitative test strips for urine hitherto only comprise a ran~e of indication up to a axilnum of 2 Q of glucose/100 ml of urine~ they are un-suitable for day in day-out routine testing, since higher glucose concentrations should also be de~ected.
In order to get round this a'isadvantage, the use of a diLuting de~ice has been proposed t~orge and Schlebusch, "t1edizinische Klinik" 75, 537-591, '1980~.
~;lutir-g devices of this type are disadvan~ageous for simple routine deterlnination, since man-,pulationc are expected of the unpractised diabetic, ~lith ~hich he is ~ ~?t --not con~ersant (pipetting, diluting and evalua-ting). The hygienic requirements are Yery stri~t and cannot al~lays be fulfilled~
In contrast, it is intended to provide the dia-betie with a simple tes~, which is easy to learn, ishygienieally aceeptable ancl ~Ihich eomprises as largD a eoncentration range as possible.
Thus the objeet of the present invention was an improved agent for determining glucose in biological fluids~
It is kno~n, from Bioehem~ Journal 42, 221 (1948), that glueose oxidase is inhibited as much as 13 % by sodium nitrate~ This inhibitory effeet is so small for glucose test strips, in ~hich a large exeess of enzymes 1~ ;s employed, that no use has hilherto Deen made of il.
Our own experinents showed that a eorresponding rapid test for deterrnining glucose ;s not inhibited by nitrates.
Conversely, it has no~ been found, surprisingly, that by impregnating glucose test strips ~ith nitra~ces, their range OT indication is signi-ficantl) enlarged, partieular~y uhen hydrophobic films (IJ~ ~lishinsky in Quality Control ;n Clinical Chermistry; Amido, Rosalhi and ~. Kampen, page 425, de Gruyter, Berlin 1975) are used.
By this means the range of indica~:ion of 2 CUstOnlary semiquant;tative test reg;on, ~Ihich extends to a max;murr, of a'~out 2 9 o~ glucose/1~0 ;nl of urine, can be extended to rnore than , g of glucose/lOO rnl of urine. These modi-fied tesc strips chus comply, in respect of their range of use, with the rec-uirements placeo on a sirnple dia~nos~:ic S -aid by a diabetir. Furthermore~ a ~est strip of this type fulfils the hygienic dernands~
Furthermore, it has been found that ~he resist~
ance of these test strips to ligllt can be improved oy the 5 ~ddit;on of UV absorbers.
The object described above has now been achieved by ~he agent containing, in addition ;o the cus-tomary components, a nitrate and, optionally, a llV absorber.
Accordingly, the inveneion relates to an agerlt 10 ~or the detection of glucose in biological fluids, essen-tially composed of an adsorbent matrix~ containing a chromogen, a glucose oxidase, a peroxidase and, option-ally, a stabilizer, which contains a nitrate and, optionally, a UV absorber.
~h. inver,tior~ furthcr rclate~ to the use o-f ~rnmon-ium nitrate or a nitrate of an element of the first group of the periodic table, ammonium and sodi~m nitrate being preferred. Examples of UV absorbers which can be used are the derivatives of benzophenone, of benzoic acid, 20 oxalic acid or of benzotriazine.
Compounds of this type are described in "IJllrllanns Enzy~;lopadie der technischen Chemie" ~lJllrnann's Encyclo-pedia of Industrial Chemistry), ~th edition, volu~e 15 pa~e 259r Verla~ Chemier Weinheim, Federal Republic of 25 Germany. 2,2'-Dihy~roxy-4-methoxybenzophenone (Cyasorb UV 24, Arnerican Cyanamide)t 2-~ydroxy-4-methoxybenzo~
phenone-5-sulfollic acid (UV absorber HMBS "Riedel", Riedel-de Haen AG, SeeLze, Federal Rep~blic of Gerrr!any) or dihydroxybenzGpllenone are preferred.
The agent accordin~ to the invention is thus com-posed o~ an ~idsorbing support matrix, in i~hich a glucose oxidase, a peroxidase, a chromogen, a i~ater-soluble nitrate and, optionally, a UV absorber are impregnat2di.
A(l absorbent plane structures of natural or synthetic or;~in can be used as the support matrix, such as, for example, non-uoven fabrics, papers, asbes~os or polymer f;lms.
The agent is advansageously prepared such that the chromogen, the buffer and the enzymes, together with the nitrate, in aqueous solution are appliecl to the test pciper. The UV absorber can also be dissolved in this irn-pregnating solution. I~ is also possible to apply the UV
absorber i~ith a iJater repelling agen~c to the support ma~rix in a further steo. The sequence of imprec~rla~ion steps is arbitrary; it is also possible to start uith the application of the i~ater-repel~ing ~igent. A process has proved effective in uhich initially an indicator base paper is impregnated ~ith chromogen, buffer, enzymes, ~etting agent, stabilizer, a i~ater soluble nitrate and a UV absorber. Then a further UV absorber and a ~Jater-repelling a~ent, for example ethylcellulose, dissolved in an organic solvent, ~hich is not miscible with Jater, such a~ toluene, are applied.
The preferreo process for ~he ?repciraiion of an agen. according to the .nvention ~hus co~priscs t~o steps.
hfter drying~ the reaction support is lar,ina~ecl on~o a plastic film for easier manipulatiorl.
The amount of nitrate :sed in each case is no~ critical; it can be adjusted to sui~ the particular envisa~ed use, that is to say the range of concentration to be co~ered. It can be between 0.1 and 7 X Sv:~) rela-tive to ammonium nitrate. A concentrat;on bet~leen 0.3 S and ~.0 X (v:~3 is preferred.
The amount ~f UV absorber used is also not criti-cal~ ln general, a larger amount of UV absorber is emplo~ed for a larger amount of nitrate. The maximum con-centration of the UV absorber is limited by ;-ts solubility in the solvent chosen. The cor,centrations can vary between Or1 and ~.0 % ~:v), however~ a concentration range betweel- 0.4 and 3.0 X (~:v) is preferred.
In order to use the agent descrihed, it is br;efly dipped into the ~u;d to be tested. h graded ~5 coLor reaction develops, depending on the content of glu-cose in the fluid, which can be graded semiquanti~atively up to 5 9 of glucose/100 ml oF urine.
The advantage of the invention emerged f-rom com-para~ive investigat;ons w;th rapid diagnostic aids accord-in~ to the state of the ar~.
Three test strip papers ~ere prepared accordingto Examples 1 to 3, of which 1 a represents the state of the art. 1 b was prepared using a water-soluble ni~ra-.e~
1 c corresponds to 1 b, but the paper was additionall ;mpregnated ~lith a UV absorber.
The follo~;nrJ tests ~ere carried out ~ith the f;nished p 2 p ers Paperc 1 a, 1 b and 1 c were eYposed to sunli~ht~
After about 20 l~inutes of this, 1 b riscolored to such ~L ~5 ~
an intense green that it gave a false positi~e result on subsequent ~etting ~/ith a glucose-free solotion. In con-trast, papers 1 a and 1 c did not change their original color even after 60 rnin~
In a further series of tests, papers 1 A ancl 1 c uere dipped in samples of urine ~Ihich contained 50, 15n, 500, 1,500~ 3,000 and 5,000 mg of gluGose/100 ~nl oF
urine. The conc~ntration s~eps up to 1~,500 Ing/dl could be easily clifferentiated with paper 1 a, ~hiLst the two 10 higher glucose concentrations ~ere not distinguished. In contrast, the entire spectrum from 50 mg of glu~ose/100 ml oF urine up to 5,000 mg of glucose/100 ml of urine could be differentiated with paper 1 c.
The invention is illustrated in more detail by 1~ means of the -;ollo~Jing examples.
Example 1 t Paper 1 a _ .
Solution 1 The following lJere dissolved in 100 ~nl of 0.1 M citrate buffer, pH 5.5:
0.5 cg of gela-tin 20 7.0 mg of tartrazine 0.6 g oF o-tolidinc dihydrochloride 0.2 g of peroxidase 0.~ 9 of glucc)se oxidase 1,0 g o~ polyethylene gLycol 1500.
Paper 2316 of Schle;cher anci Schull, Dassel, Federal Republic of Gernlany, ~,las inlpregna~ed ~ith this irnpregnation solution. After drying at 80C in an oven, the paper uas subjected to a second3ry iMpregnation ~/-ith Solution 2 containing 0 6 CJ Of ethylcellulose in 1G0 ~nl : . .
s-j ~Lr~
of toluene.
Exa,-,lple 2 ,t Paper 1 b A pa~er 1 b ~as prepared in accordance ~ith Exampl~ 1, but an add;tional 2 9 of ammonium nitrate were 5 dissGlved in solution 1. The secondary impregnation was h so lu~ i on 2 .
Example 3 / Paper 1 c _ _ _ Th e f o l lOh'i ng were additionally di sso lved i n 100 ml of ~he impregnation solution 1 according to Example 1:
2.0 ~ o~ ammonium nitrate and 1rO ~ OT UV absorber HMBS "Riedel"~
The following lere dissolved in 100 ml of tol~ene for ~he seconclary impregnation:
0.5 ~, of ethylcellu!ose and 2.0 9 of Cyasorb, Cyanamide.
Papers 1 a, 1 b and 1 c showed ~he propelties described abuve.
prises an adsorbent matrix~ a glucose oxidase, a peroxid-ase and a chromogen as re3gent anci contains a nitrate and, optionally~ a UV absorber.
Diabetes mellitus is a chronic disease ~hich must be continuously monitored. It has proved to be reason~
able for the diabetic to monitor his o~ln metabolic status afte,r ins~ruct;on by ~he phys;cian ;n order to be able, 1~ if necessary, immediately to adjust the necessary insulir dose ~o suit ~he changing requiremcnts. The check of metaboLism is carried out sush that the conten~ of ~lucose in the urine or in the blood is routineLy determined. In general, the diabetic determines his O~il urinary glucose value semiquantitatively, whilst~ in contrast, quantitative measuremen~ of blood sugar is carried out by an expert.
The determination of urinary glucose sl1ould cover a concentration rancge up to about 5 g of c3lucose/100 ml ~- ~f urine.
hi present, there arè t,to met,hods for rapid semi--~uantitative C~etermiQatiOn o-F c3lucose:
1. Test ;ablecs accordincJ to the non-specific reduction r,letllocl o-F ~ EDlKr and ~ Test strips ~hich are irDprec~nated ~ich c~lucoce oxidase, ' ' 3 which reacts soecifically, a chromogen and a peroxidase.
The glucose oxidase in the test strips catalyzes the ox;dation of glucose with oxygen to for~, gLuconolact-one and hydrogen peroxide. In a subsequent non-speçifi~
reaction~ ehe latter oxidizes a chromogen, ~i~h peroxid-ase catalysis, to g;ve a dyestuff.
The t~o methods ~entioned are associated uith dis-ad~antages: -! Th2 reduction method is non-specific, since other reducing com?ounds, such as, for exanple, galactose, are also de~ected. Furtherrrlore, i~ is necessary to use corrosive reagents for this rnethod (G. Friedrich~ "Der ;nformierte Arzt" 8 (9), Off-print, 1~80). In addition, ~he reaction is associa~ed ~ith a not inconsiderable 'l~ evolution ~f heat alld ~iih an interle~ g forr,7atiun of foam. The performance of ~he tests ~lith tablets and ~est tubes is elaborate, inconvenient and costly.
The same applies to the second rnethod of deterrnin-at;on~ S;nce thc semiquantitative test strips for urine hitherto only comprise a ran~e of indication up to a axilnum of 2 Q of glucose/100 ml of urine~ they are un-suitable for day in day-out routine testing, since higher glucose concentrations should also be de~ected.
In order to get round this a'isadvantage, the use of a diLuting de~ice has been proposed t~orge and Schlebusch, "t1edizinische Klinik" 75, 537-591, '1980~.
~;lutir-g devices of this type are disadvan~ageous for simple routine deterlnination, since man-,pulationc are expected of the unpractised diabetic, ~lith ~hich he is ~ ~?t --not con~ersant (pipetting, diluting and evalua-ting). The hygienic requirements are Yery stri~t and cannot al~lays be fulfilled~
In contrast, it is intended to provide the dia-betie with a simple tes~, which is easy to learn, ishygienieally aceeptable ancl ~Ihich eomprises as largD a eoncentration range as possible.
Thus the objeet of the present invention was an improved agent for determining glucose in biological fluids~
It is kno~n, from Bioehem~ Journal 42, 221 (1948), that glueose oxidase is inhibited as much as 13 % by sodium nitrate~ This inhibitory effeet is so small for glucose test strips, in ~hich a large exeess of enzymes 1~ ;s employed, that no use has hilherto Deen made of il.
Our own experinents showed that a eorresponding rapid test for deterrnining glucose ;s not inhibited by nitrates.
Conversely, it has no~ been found, surprisingly, that by impregnating glucose test strips ~ith nitra~ces, their range OT indication is signi-ficantl) enlarged, partieular~y uhen hydrophobic films (IJ~ ~lishinsky in Quality Control ;n Clinical Chermistry; Amido, Rosalhi and ~. Kampen, page 425, de Gruyter, Berlin 1975) are used.
By this means the range of indica~:ion of 2 CUstOnlary semiquant;tative test reg;on, ~Ihich extends to a max;murr, of a'~out 2 9 o~ glucose/1~0 ;nl of urine, can be extended to rnore than , g of glucose/lOO rnl of urine. These modi-fied tesc strips chus comply, in respect of their range of use, with the rec-uirements placeo on a sirnple dia~nos~:ic S -aid by a diabetir. Furthermore~ a ~est strip of this type fulfils the hygienic dernands~
Furthermore, it has been found that ~he resist~
ance of these test strips to ligllt can be improved oy the 5 ~ddit;on of UV absorbers.
The object described above has now been achieved by ~he agent containing, in addition ;o the cus-tomary components, a nitrate and, optionally, a llV absorber.
Accordingly, the inveneion relates to an agerlt 10 ~or the detection of glucose in biological fluids, essen-tially composed of an adsorbent matrix~ containing a chromogen, a glucose oxidase, a peroxidase and, option-ally, a stabilizer, which contains a nitrate and, optionally, a UV absorber.
~h. inver,tior~ furthcr rclate~ to the use o-f ~rnmon-ium nitrate or a nitrate of an element of the first group of the periodic table, ammonium and sodi~m nitrate being preferred. Examples of UV absorbers which can be used are the derivatives of benzophenone, of benzoic acid, 20 oxalic acid or of benzotriazine.
Compounds of this type are described in "IJllrllanns Enzy~;lopadie der technischen Chemie" ~lJllrnann's Encyclo-pedia of Industrial Chemistry), ~th edition, volu~e 15 pa~e 259r Verla~ Chemier Weinheim, Federal Republic of 25 Germany. 2,2'-Dihy~roxy-4-methoxybenzophenone (Cyasorb UV 24, Arnerican Cyanamide)t 2-~ydroxy-4-methoxybenzo~
phenone-5-sulfollic acid (UV absorber HMBS "Riedel", Riedel-de Haen AG, SeeLze, Federal Rep~blic of Gerrr!any) or dihydroxybenzGpllenone are preferred.
The agent accordin~ to the invention is thus com-posed o~ an ~idsorbing support matrix, in i~hich a glucose oxidase, a peroxidase, a chromogen, a i~ater-soluble nitrate and, optionally, a UV absorber are impregnat2di.
A(l absorbent plane structures of natural or synthetic or;~in can be used as the support matrix, such as, for example, non-uoven fabrics, papers, asbes~os or polymer f;lms.
The agent is advansageously prepared such that the chromogen, the buffer and the enzymes, together with the nitrate, in aqueous solution are appliecl to the test pciper. The UV absorber can also be dissolved in this irn-pregnating solution. I~ is also possible to apply the UV
absorber i~ith a iJater repelling agen~c to the support ma~rix in a further steo. The sequence of imprec~rla~ion steps is arbitrary; it is also possible to start uith the application of the i~ater-repel~ing ~igent. A process has proved effective in uhich initially an indicator base paper is impregnated ~ith chromogen, buffer, enzymes, ~etting agent, stabilizer, a i~ater soluble nitrate and a UV absorber. Then a further UV absorber and a ~Jater-repelling a~ent, for example ethylcellulose, dissolved in an organic solvent, ~hich is not miscible with Jater, such a~ toluene, are applied.
The preferreo process for ~he ?repciraiion of an agen. according to the .nvention ~hus co~priscs t~o steps.
hfter drying~ the reaction support is lar,ina~ecl on~o a plastic film for easier manipulatiorl.
The amount of nitrate :sed in each case is no~ critical; it can be adjusted to sui~ the particular envisa~ed use, that is to say the range of concentration to be co~ered. It can be between 0.1 and 7 X Sv:~) rela-tive to ammonium nitrate. A concentrat;on bet~leen 0.3 S and ~.0 X (v:~3 is preferred.
The amount ~f UV absorber used is also not criti-cal~ ln general, a larger amount of UV absorber is emplo~ed for a larger amount of nitrate. The maximum con-centration of the UV absorber is limited by ;-ts solubility in the solvent chosen. The cor,centrations can vary between Or1 and ~.0 % ~:v), however~ a concentration range betweel- 0.4 and 3.0 X (~:v) is preferred.
In order to use the agent descrihed, it is br;efly dipped into the ~u;d to be tested. h graded ~5 coLor reaction develops, depending on the content of glu-cose in the fluid, which can be graded semiquanti~atively up to 5 9 of glucose/100 ml oF urine.
The advantage of the invention emerged f-rom com-para~ive investigat;ons w;th rapid diagnostic aids accord-in~ to the state of the ar~.
Three test strip papers ~ere prepared accordingto Examples 1 to 3, of which 1 a represents the state of the art. 1 b was prepared using a water-soluble ni~ra-.e~
1 c corresponds to 1 b, but the paper was additionall ;mpregnated ~lith a UV absorber.
The follo~;nrJ tests ~ere carried out ~ith the f;nished p 2 p ers Paperc 1 a, 1 b and 1 c were eYposed to sunli~ht~
After about 20 l~inutes of this, 1 b riscolored to such ~L ~5 ~
an intense green that it gave a false positi~e result on subsequent ~etting ~/ith a glucose-free solotion. In con-trast, papers 1 a and 1 c did not change their original color even after 60 rnin~
In a further series of tests, papers 1 A ancl 1 c uere dipped in samples of urine ~Ihich contained 50, 15n, 500, 1,500~ 3,000 and 5,000 mg of gluGose/100 ~nl oF
urine. The conc~ntration s~eps up to 1~,500 Ing/dl could be easily clifferentiated with paper 1 a, ~hiLst the two 10 higher glucose concentrations ~ere not distinguished. In contrast, the entire spectrum from 50 mg of glu~ose/100 ml oF urine up to 5,000 mg of glucose/100 ml of urine could be differentiated with paper 1 c.
The invention is illustrated in more detail by 1~ means of the -;ollo~Jing examples.
Example 1 t Paper 1 a _ .
Solution 1 The following lJere dissolved in 100 ~nl of 0.1 M citrate buffer, pH 5.5:
0.5 cg of gela-tin 20 7.0 mg of tartrazine 0.6 g oF o-tolidinc dihydrochloride 0.2 g of peroxidase 0.~ 9 of glucc)se oxidase 1,0 g o~ polyethylene gLycol 1500.
Paper 2316 of Schle;cher anci Schull, Dassel, Federal Republic of Gernlany, ~,las inlpregna~ed ~ith this irnpregnation solution. After drying at 80C in an oven, the paper uas subjected to a second3ry iMpregnation ~/-ith Solution 2 containing 0 6 CJ Of ethylcellulose in 1G0 ~nl : . .
s-j ~Lr~
of toluene.
Exa,-,lple 2 ,t Paper 1 b A pa~er 1 b ~as prepared in accordance ~ith Exampl~ 1, but an add;tional 2 9 of ammonium nitrate were 5 dissGlved in solution 1. The secondary impregnation was h so lu~ i on 2 .
Example 3 / Paper 1 c _ _ _ Th e f o l lOh'i ng were additionally di sso lved i n 100 ml of ~he impregnation solution 1 according to Example 1:
2.0 ~ o~ ammonium nitrate and 1rO ~ OT UV absorber HMBS "Riedel"~
The following lere dissolved in 100 ml of tol~ene for ~he seconclary impregnation:
0.5 ~, of ethylcellu!ose and 2.0 9 of Cyasorb, Cyanamide.
Papers 1 a, 1 b and 1 c showed ~he propelties described abuve.
Claims (9)
OR PRIVILEGE IS CLAIMED ARE DEFINED AS FOLLOWS:
1. An agent for detecting glucose in biological fluids, which essentially comprises an adsorbent matrix containing a chromogen, a glucose oxidase, a peroxidase and a nitrate.
2. An agent as claimed in claim 1 which also contains a UV absorber.
3. An agent as claimed in claim 1 wherein the nitrate is a nitrate of an element of the first group of the periodic table or is ammonium nitrate.
4. An agent as claimed in claim 2 wherein the UV absorber is a derivative of benzoic acid, of benzophenone, of oxalic acid or is a benzotriazine.
5. An agent as claimed in claim 3 wherein the nitrate is ammonium or sodium nitrate.
6. An agent as claimed in claim 4 wherein the UV absorber is 2,2'-dihydroxy-4-methoxybenzophenone and 2-hydroxy-4-methoxy-benzophenone-5-sulfonic acid.
7. A process for the preparation of an agent as claimed in claim 1 which comprises treating a support with an aqueous solution which contains the ntirate, the glucose oxidase, the peroxidase and the chromogen.
8. A process as claimed in claim 7 in which, after drying, treating the support with a solution which is not miscible with water and which contains a further UV absorber and a water-repelling agent.
9. A process as claimed in claim 8 in which the solution also contains a UV absorber.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DEP3142862.2 | 1981-10-29 | ||
| DE19813142862 DE3142862A1 (en) | 1981-10-29 | 1981-10-29 | "AGENT FOR DETECTING GLUCOSE IN BIOLOGICAL LIQUIDS" |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1185153A true CA1185153A (en) | 1985-04-09 |
Family
ID=6145088
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000414392A Expired CA1185153A (en) | 1981-10-29 | 1982-10-28 | Agent for detecting glucose in biological fluids |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP0078971B1 (en) |
| JP (1) | JPS5881800A (en) |
| AT (1) | ATE12403T1 (en) |
| AU (1) | AU8984982A (en) |
| CA (1) | CA1185153A (en) |
| DE (2) | DE3142862A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023172715A1 (en) | 2022-03-11 | 2023-09-14 | Sun Chemical Corporation | Method of determining glucose concentration |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DD247808C2 (en) * | 1984-04-09 | 1989-01-11 | Dresden Arzneimittel | TEST STRIPS FOR RECEIVING BLOOD SUGAR DAY PROFILES AND METHOD FOR THE PRODUCTION THEREOF |
| DE3434822A1 (en) * | 1984-09-22 | 1986-04-03 | Bayer Ag, 5090 Leverkusen | MEMBRANE FOR REAGENT CARRIER LAYERS, METHOD FOR THE PRODUCTION THEREOF, AND THEIR USE IN ANALYTICAL AGENTS AND ANALYZING METHOD |
| JP2660837B2 (en) * | 1987-09-22 | 1997-10-08 | 栄研化学株式会社 | Test strip for detecting peroxide active substances |
| US8173437B2 (en) * | 2005-06-10 | 2012-05-08 | Mannatech, Incorporated | Rapid serum sugar biomarker assay of rheumatoid arthritis |
| CA2564666A1 (en) | 2005-10-25 | 2007-04-25 | F. Hoffmann-La Roche Ag | Fluorescence spectroscopy in absorbing media |
| WO2007097027A1 (en) | 2006-02-27 | 2007-08-30 | Fujitsu Limited | Degeneration controller and degeneration control program |
| JP5451087B2 (en) * | 2009-01-26 | 2014-03-26 | エヌイーシーコンピュータテクノ株式会社 | Fault processing apparatus and method |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3964871A (en) * | 1974-12-18 | 1976-06-22 | Becton, Dickinson And Company | Method and device for detecting glucose |
| IT1099355B (en) * | 1978-10-12 | 1985-09-18 | Sclavo Inst Sieroterapeut | COMPOSITION SUITABLE FOR THE DETERMINATION OF GLUCOSE IN KINETICS |
-
1981
- 1981-10-29 DE DE19813142862 patent/DE3142862A1/en not_active Withdrawn
-
1982
- 1982-10-26 DE DE8282109853T patent/DE3262821D1/en not_active Expired
- 1982-10-26 EP EP82109853A patent/EP0078971B1/en not_active Expired
- 1982-10-26 AT AT82109853T patent/ATE12403T1/en not_active IP Right Cessation
- 1982-10-28 CA CA000414392A patent/CA1185153A/en not_active Expired
- 1982-10-28 JP JP57188269A patent/JPS5881800A/en active Pending
- 1982-10-28 AU AU89849/82A patent/AU8984982A/en not_active Abandoned
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023172715A1 (en) | 2022-03-11 | 2023-09-14 | Sun Chemical Corporation | Method of determining glucose concentration |
Also Published As
| Publication number | Publication date |
|---|---|
| EP0078971A1 (en) | 1983-05-18 |
| AU8984982A (en) | 1983-05-05 |
| DE3142862A1 (en) | 1983-05-11 |
| ATE12403T1 (en) | 1985-04-15 |
| DE3262821D1 (en) | 1985-05-02 |
| EP0078971B1 (en) | 1985-03-27 |
| JPS5881800A (en) | 1983-05-17 |
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| MKEX | Expiry |