CA1123228A - Detection of live insects in a commmodity sample - Google Patents
Detection of live insects in a commmodity sampleInfo
- Publication number
- CA1123228A CA1123228A CA318,354A CA318354A CA1123228A CA 1123228 A CA1123228 A CA 1123228A CA 318354 A CA318354 A CA 318354A CA 1123228 A CA1123228 A CA 1123228A
- Authority
- CA
- Canada
- Prior art keywords
- carbon dioxide
- commodity
- sample
- cell
- closed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/497—Physical analysis of biological material of gaseous biological material, e.g. breath
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/12—Condition responsive control
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- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Food Science & Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
ABSTRACT OF THE DISCLOSURE
A system for detecting live insects in a commodity sample features forming a closed-loop between a stored quantity of fluid and a sample cell and a reference cell.
The closed-loop and the stored fluid ensure a controlled environment during incubation and analysis of the commodity sample. This controlled environment achieves a greater sensitivity and reproducibility than prior systems.
.
A system for detecting live insects in a commodity sample features forming a closed-loop between a stored quantity of fluid and a sample cell and a reference cell.
The closed-loop and the stored fluid ensure a controlled environment during incubation and analysis of the commodity sample. This controlled environment achieves a greater sensitivity and reproducibility than prior systems.
.
Description
, 1 Field of the Invention This invention relates to method and analysis of materials, and more particularly, to a method and system for analyzing a commodity sample for the presence of carbon dioxide as an indication of live insec-t infestation.
2 Background of the Invention Recently, a carbon dioxide analyzing system has been developed for detecting live insects in commodity samples. A commodity sample is introduced into a commodity test chamber (CTC~, and the system is closed to the environment for a given period of time. During this incubation time, any insects in the sample will respire or otherwise give off carbon dioxide. After this given period of time, the system is opened to the ambient air,-and the carbon dioxide in the test chamber is forced under pressure or vacuum to a sample cell. The carbon dioxide level in this sample cell is then compared with a reference level carbon dioxide in a reference cell. Infestation will be indicated in the commodity sample, when the two levels ~ -do not coincide.
The above system i5 described in the literaturè in the following articles: "Detection of Hidden Insects" by William A. Bruce and Marion W. Street, Jr., Department of Agriculture, Washington, D. C., 18 June, 1975, (PB 248 002);
Department of Agriculture Report (ARS-S-85) by William A.
Bruce and Marion W. Street, Jr., March, 1976; and "CO2 Analyzer Detects Insects Hidden in Foods" by William A. Bruce and Marion W. Street, Jr., Food Engineering, February, 1976.
mb/~r - 2 -,~
The above system i5 described in the literaturè in the following articles: "Detection of Hidden Insects" by William A. Bruce and Marion W. Street, Jr., Department of Agriculture, Washington, D. C., 18 June, 1975, (PB 248 002);
Department of Agriculture Report (ARS-S-85) by William A.
Bruce and Marion W. Street, Jr., March, 1976; and "CO2 Analyzer Detects Insects Hidden in Foods" by William A. Bruce and Marion W. Street, Jr., Food Engineering, February, 1976.
mb/~r - 2 -,~
3~8 While this prior system has made a major contribution in the art of insect detection, certain shor-t-comings have been noted to exist. This system has been found to give results which are sometimes neither accurate nor reproducible, as the system is open to the ambient surroundings during the transfer of the carbon dioxide from the commodity test chamber (CTC) to the sample cell.
The ambient air is sucked into the system as the carbon dioxide in the test chamber is pumped to the sample cell.
The ambient air will normally contain the same level of carbon dioxide as in the reference cell, which was open to ambient air prior to incubation. If such is the case, the system will give accurate results. However, this is not always true. Ambient conditions may change during incubation, as particularly so when people breathe or smoke near the system inlet, so as to cause a higher carbon dioxide con-centration to exist in the air sucked into the sample cell. Thus, false readings often result.
The reliability of the system is dramatically impaired by these extraneous concentrations of carbon dioxide, particularly since very low levels o~ carbon dioxide are to be measured against a high reference level. Thus, a person breathing near the system can generate enough carbon dioxide to ~ive an indication of infestation, when in actuality no insects are present in the`sample.
A single insect may generate only several parts per million (ppm) of carbon dioxide in a few minutes. This minute amount of dm~ 3 -~3 ~
;~.`
1~2~2~
carbon dioxide must ~e measured against an ambient back-ground of approximately 270-~00 ppm. Thus, even the slightest change in ambient surroundings of the system will greatly influence the accuracy and reliability of the analysis.
The present invention seeks to overcome the above drawbacks of the prior art by providing a controlled background level of carbon dioxide in both the sample ana reference cells. This controlled environment ensures tha-t any change in the level of carbon dioxide in`the sample cell is due entirely tc~ infestation, and not to any changes in the ambient surroundings. The invention contemplates providing a closed-loop system to avoid extraneous carbon dioxide from entering the system and, furthermore, supplying each of the sample and reference cells with a controlled amount of background carbon dioxide, whereby the reference, or background, level of carbon dioxide remains constant throughout the analysis.
It is known to have closed loops in okher types of analyzing systems, such as described in "A Rapid and Speclfic Method for the Estimation of Glucose Using an Oxygen Electrode and Simple Differentiating Circuit" by ~`
H. L. J. ~5akin, P. J. Warren and J. D. Edridge r Clinica Chimica Acta, 84 (1978), pages 137-143. However, such prior systems have used the closed loop as a means to save the enzyme for reuse, and not to precisely control the system parameters during the analysis process.
SUr`~MARY OF THE INVENTION
The present invention contemplates positive control of the environment during actual tests by effecting the analysis in s;, . f mb/~
3Z~3 closed-loop system, whereby the background level of the condition to be measured, e.g., the presence of carbon dioxide, is precisely controlled.
According to one aspect the present inven-tion provides a system for detecting the infestation of live insects within a commodity sample comprising: means for storing a fluid having a reference level of carbon dioxide;
at least one commodity chamber for containing a commodity sample to be tested for infestation by live insects, the live insects generatina carbon dioxide to be analyzed;
a sample cell associated with the commodity chamber for receiving any generated carbon dioxide from the commodity chamber; a reference cell associated with the sample cell for establishing a background level of carbon dioxide in the system; first means for controlling the passage of a portion o~ the fluid containing the reference level of carbon dioxide from the storing means into the reference - cell for establishing the bac~ground level of car~on dioxide in the reference cell; second means defining a closed-loop comprising the storing means, the commodity chamber and the sample cell, means for ci.rculating a portion of the fluid containing the reference level of caxbon dioxide along the closed-loop, so as to transfer any :
carbon dioxide generated in the commodity cha~ber to the sample cell; and means for measuring the total carbon dioxide level in the sample cell in respect of the carbon dioxide level in the reference cell, whereby the presence of live insects in the commodity sample is detected.
dm:l~3 5 2~
According to a further aspect the present invention provides a method of detecting the infestation of live insects within a commodity sample, comprisin~
the steps of: (a) storing a given quantity of fluid containing a reference level of carbon dioxide; (b) cir-culating a portion of the stored fluid through a reference cell in a controlled manner to establish a controlled level of carbon dioxide in the reference cell; (c) incubating a commodity sample disposed within a commodity chamber to generate a sample level of carbon dioxide; (d) circulating a portion of the stored fluid in a controlled manner through the commodity chamber and the sample cell and then to the commodity chamber, in closed-loop fashion, so as to transfer any carbon dioxide generated in the commodity chamber to the sample cell; and (e) measuring any difference between carbon dioxide levels in the sample cell and the reference cell, respectively, to detect the presence of any living insects in the commodity sample.
DESCRIPTION OF THE DRAWINGS
Preferred embodiments of the invention will now be described by way of example with reference to the accompanying drawings, in which:
Fig. 1 shows a schematic diagram of the system of the invention, with the sample and re~erence cells arranged in parallel, and wherein;
Fig. la depicts a typical timing diagram for operating the system of Fig. l; and Fig. lb illustrates the valving sequence for the system of Fig. l; and dm~ 6 -31~23228 Fig. 2 shows a schematic diagram of the system of Fig. 1, with the sample and reference cells arranged in series, and wherein;
Fig. 2a depicts a typical timing diagram for operating the system of Fig. 2; and dm:J~ 6a -~123ZZ8 Fig. 2b illustrates the valving sequence for the system of Fig. 2.
DETAILED DESCRIPTION
Generally speaking, the invention is for a me~hod and system for detecting the infestation of live insec-ts within a commodity sample. A given quantity of fluid is stored. Generally, this fluid is air having a certain content of carbon dioxide. A portion of the stored fluid is circulated through a reference cell of the system in a controlled manner to establish a controlled level of carhon dioxide. The co~modity sample is deposited in a commodity test chamber to allow the sample to incubate, or otherwise generate carbon dioxide. A portion of the stored fluid is circulated through the commodity test chamber in a controlled manner in order to transfer any of the generated ;~, carbon dioxide from the commodity chamber -to a sample cell. ~ ' r The sample cell and the reference cell are analyzed for a difference in carbon dioxide level. The presence of living insects in the commodity sample will be detected by a difference in carbon dioxide level between the sample cell and the reference cell.
Now referring to Fig. 1, a pneumatic circuit diagram is shown which illustrates the present invention.
The system comprises a container 9, filled with ambient air.
A conduit 1~ extends from container 9 and divides into two conduit paths lOa and lQb via junction 15. Conduit lOa is connected to a reference cell 11 via a filter 12. A portion of the air in container 9 is circulated through conduit lOa ~ , by means of vacuum pump 13. A flow meter 14 connected in conduit lOa controls the rate of flow through the reference ' cell 11. Conduit lOb carries a por-tion of the air in container 9 to a first valve V4. Depending upon the mb/,;- _ 7 1 23~2~
position of valve V4, the air will be directed to either valve V3 or to a second one of three commodit~ test chambers ~CTC), further designated with Roman numerals I, II and III, respectively. Valve V3 also has two positions, whereby the air may be directed to commodity chambers I
or III, respectively.
Two valves Vl and V2 are on the downstream side of the commodity test chambers CTC I and CTC III and of commodity test chamber CTC II~ respectively, and direct the circulating air from conduit lOb to a sample cell 16 via a filter 17. Filters 12 and 1? trap dust and extraneous matter from entering the cells 11 and 16~ respectively.
The pump 13 circulates air in conduit lOb as well as conduit lOa. A flow meter 18 in conduit lOb regulates the flow rate through the sample cell 16. ~ vacuum ~auge 19 is in the conduit lOb to gauge the pressure in the system and provide an indication of clogging of the filter. Air from both conduits lOa and lOb flow at the same rate in order to establish an equal background level of carbon dioxide in each cell 11 and 16, respectively. The air ~rom conduits lOa and lOb is returned to the sealed container 9 via conduit lOc. An analyzer 20 is provided to measure the ~iference in the carbon dioxide level difference in sample cell 16 and reference cell 11.
A container of carbon dioxide 21 is connected to a dosing valve V5, to introduce a given quantity or charge of carbon dioxide into conduit lOb. This charge is subsequently introduced into sample cell 16 via the valves Vl, V2, V3 and V4, so as to calibrate the system.
Fig. 2 is a pneumatic diagram for an alternate circuit arrangement for the invention. The sample cell 16 and reference cell 11 are now arranged in series with ~;~
. ~.
~l~2;3Z2~
respect to container ~ instead of in parallel (Fig. l).
The system of Fig. 2, however, operates in the exact fashion as the system in Fig. l. ~ike components have been given the same designa-tions in Fig. 2 as they had in Fig. l. Because the system is a series flow circuit, only one metering valve 18 is required to regulate the flow through the sample and reference cells.
OPERATION OF THE INVENTION
- The operation of the systems shown in Figs. 1 and 2, will be described with reference to Figs. la, lb, 2a and 2b. While these diagrams show how three commodity test chambers CTC I, CTC II, and CTC III may be opera~ed in a phased sequence, it should be appreciated that only one commodity test chamber may be used in the system consistent with the invention.
The commodity test cham~bers CTC I, CTC II, and - CTC III are initially filled with grain or another similar type commodity to be tested for infestation by live insects.
These chambers are initially open at each end, and are sealed ~rom the ambient surroundings ~hen they are connected into the system. When so connected, they form part of a closed loop defined by conduits 10b and 10c.
In the operation described in Figs. la and lb, the three filled commodity test chambers CTC I, CTC II, and CTC III are connected into the system and subsequently analyzed in a phased sequence. A typical cycle for each commodity test chamber includes an initial purging or flushing of the chamber with air from container ~. During the purging of commodity test chamber CTC I, for example, the valves Vl, V2, V3, and V4 are all deenergized to provide a fluid flow connection between commodity test chamber CTC I and the system to complete;the-~closed loop.
mh/ ~ - 9 -~232;~
At the same time, the other commodit~ test chambers CTC II
and CTC III are not fluidically connected into the system.
Rather, as illustrated in Fig. la, sample is being changed in commodity -test chamber CTC II and commodi-ty test chamber CTC III is in the incubation phase.
The purging of commodity test chamber CTC I
accomplishes two purposes: (a) it equilibrates the system by mixing any entrapped air and carbon dioxide in the test chamber with the air in the rest of the closed loop, and (b) the sample in the commodity test chamber is ~ashed clean of any residual carbon dioxide, so that the subsequent measurement of carbon dioxide in the commodity test chamber CTC I ~ill be entirely in respect of live insect respiration.
After the commodity test chamber CTC I has been purged, valves V2 and V4 are energi~ed, thus isolating commodity test chamber CTC I from the rest of the system.
During this time, commodity test chamber CTC II is fluidicall~
introduced into the closed loop and purged by the continuously circulating air, via pump 13 (see valve se~uence diagram Fi~. lb). It will be seen from Fig. lb that the commodity test chamber CTC I is now in its incubation phase. The incubation phase of commodity test chamber CTC I allows for the generation of carbon dioxide by live insects over a time period. Fig. la sho~s a three minute incubation time, but naturally, all the times of these cycles can be rearranged to suit the user of the system.
After the incubation phase of commodity -test chamber CTC I, valves Vl and V3 are energi~ed to allow air to flow through such chamber. This is the one minute analysis time designated R in Fig. la. The air flowing through commodity test chamber CTC I at this tiMe will sweep the contents, - including any g~enerated carbon dioxide, from such chamber , i ~
3:~28 to sample cell 16 for analysis. It will be noted that at this time commodity test chamber CTC II is startiny its three minute incubation phase, and is closed to the system (see Fig. lb).
If carbon dioxide has been generated by live insects in commodity test chamber CTC I, then the analyzer 20 will detect a difference between carbon dioxide levels in the reference cell 11 and the sample cell 16.
After the ana]ysis, the sample in commodity test cha~ber CTC I can be changed, as shown in Fig. la, by removing such cha~ber from the system.
The cycle for commodity test chamber CTC I can now be repeated for a new sample with the subsequent reintroduc-tion of such chamber into the system.
It will be seen by observing Figs. la and lb, that the testing of all three commodity test chambers, CTC I, CTC I~, and CTC III are phased with respect to each other, by operating valves Vl, V2, V3 and va in proper se~uence, so that a continuously phased s~stem of analysis is achieved.
Figs. 2a and 2b are identical to Fi~s. la and lb, respectively. Thus, it will also be observed that the series circuit of Fig. 2 can be operated in like fashion with the parallel circuit shown in Fig. 1.
The sequencing of the valves ma~ be controlled by a programmed computer to automate the system. Of course, other controls can be employed.
The sample cell 16, reference cell 11, and analyzer 20 are shown schema-tically as separate units, but are, in fact, packaged as a single unit. Such units are made hy Beckman (Model lR-215B) for example, and use infrared detection to distinguish the various differences ih carbon dioxide levels between the cells 11 and 16.
~L~23~Z8 .
Having thus described the invention, what is sought to be covered by Letters Patent is presented by the appended claims.
.. ,. '~
? ~ .
The ambient air is sucked into the system as the carbon dioxide in the test chamber is pumped to the sample cell.
The ambient air will normally contain the same level of carbon dioxide as in the reference cell, which was open to ambient air prior to incubation. If such is the case, the system will give accurate results. However, this is not always true. Ambient conditions may change during incubation, as particularly so when people breathe or smoke near the system inlet, so as to cause a higher carbon dioxide con-centration to exist in the air sucked into the sample cell. Thus, false readings often result.
The reliability of the system is dramatically impaired by these extraneous concentrations of carbon dioxide, particularly since very low levels o~ carbon dioxide are to be measured against a high reference level. Thus, a person breathing near the system can generate enough carbon dioxide to ~ive an indication of infestation, when in actuality no insects are present in the`sample.
A single insect may generate only several parts per million (ppm) of carbon dioxide in a few minutes. This minute amount of dm~ 3 -~3 ~
;~.`
1~2~2~
carbon dioxide must ~e measured against an ambient back-ground of approximately 270-~00 ppm. Thus, even the slightest change in ambient surroundings of the system will greatly influence the accuracy and reliability of the analysis.
The present invention seeks to overcome the above drawbacks of the prior art by providing a controlled background level of carbon dioxide in both the sample ana reference cells. This controlled environment ensures tha-t any change in the level of carbon dioxide in`the sample cell is due entirely tc~ infestation, and not to any changes in the ambient surroundings. The invention contemplates providing a closed-loop system to avoid extraneous carbon dioxide from entering the system and, furthermore, supplying each of the sample and reference cells with a controlled amount of background carbon dioxide, whereby the reference, or background, level of carbon dioxide remains constant throughout the analysis.
It is known to have closed loops in okher types of analyzing systems, such as described in "A Rapid and Speclfic Method for the Estimation of Glucose Using an Oxygen Electrode and Simple Differentiating Circuit" by ~`
H. L. J. ~5akin, P. J. Warren and J. D. Edridge r Clinica Chimica Acta, 84 (1978), pages 137-143. However, such prior systems have used the closed loop as a means to save the enzyme for reuse, and not to precisely control the system parameters during the analysis process.
SUr`~MARY OF THE INVENTION
The present invention contemplates positive control of the environment during actual tests by effecting the analysis in s;, . f mb/~
3Z~3 closed-loop system, whereby the background level of the condition to be measured, e.g., the presence of carbon dioxide, is precisely controlled.
According to one aspect the present inven-tion provides a system for detecting the infestation of live insects within a commodity sample comprising: means for storing a fluid having a reference level of carbon dioxide;
at least one commodity chamber for containing a commodity sample to be tested for infestation by live insects, the live insects generatina carbon dioxide to be analyzed;
a sample cell associated with the commodity chamber for receiving any generated carbon dioxide from the commodity chamber; a reference cell associated with the sample cell for establishing a background level of carbon dioxide in the system; first means for controlling the passage of a portion o~ the fluid containing the reference level of carbon dioxide from the storing means into the reference - cell for establishing the bac~ground level of car~on dioxide in the reference cell; second means defining a closed-loop comprising the storing means, the commodity chamber and the sample cell, means for ci.rculating a portion of the fluid containing the reference level of caxbon dioxide along the closed-loop, so as to transfer any :
carbon dioxide generated in the commodity cha~ber to the sample cell; and means for measuring the total carbon dioxide level in the sample cell in respect of the carbon dioxide level in the reference cell, whereby the presence of live insects in the commodity sample is detected.
dm:l~3 5 2~
According to a further aspect the present invention provides a method of detecting the infestation of live insects within a commodity sample, comprisin~
the steps of: (a) storing a given quantity of fluid containing a reference level of carbon dioxide; (b) cir-culating a portion of the stored fluid through a reference cell in a controlled manner to establish a controlled level of carbon dioxide in the reference cell; (c) incubating a commodity sample disposed within a commodity chamber to generate a sample level of carbon dioxide; (d) circulating a portion of the stored fluid in a controlled manner through the commodity chamber and the sample cell and then to the commodity chamber, in closed-loop fashion, so as to transfer any carbon dioxide generated in the commodity chamber to the sample cell; and (e) measuring any difference between carbon dioxide levels in the sample cell and the reference cell, respectively, to detect the presence of any living insects in the commodity sample.
DESCRIPTION OF THE DRAWINGS
Preferred embodiments of the invention will now be described by way of example with reference to the accompanying drawings, in which:
Fig. 1 shows a schematic diagram of the system of the invention, with the sample and re~erence cells arranged in parallel, and wherein;
Fig. la depicts a typical timing diagram for operating the system of Fig. l; and Fig. lb illustrates the valving sequence for the system of Fig. l; and dm~ 6 -31~23228 Fig. 2 shows a schematic diagram of the system of Fig. 1, with the sample and reference cells arranged in series, and wherein;
Fig. 2a depicts a typical timing diagram for operating the system of Fig. 2; and dm:J~ 6a -~123ZZ8 Fig. 2b illustrates the valving sequence for the system of Fig. 2.
DETAILED DESCRIPTION
Generally speaking, the invention is for a me~hod and system for detecting the infestation of live insec-ts within a commodity sample. A given quantity of fluid is stored. Generally, this fluid is air having a certain content of carbon dioxide. A portion of the stored fluid is circulated through a reference cell of the system in a controlled manner to establish a controlled level of carhon dioxide. The co~modity sample is deposited in a commodity test chamber to allow the sample to incubate, or otherwise generate carbon dioxide. A portion of the stored fluid is circulated through the commodity test chamber in a controlled manner in order to transfer any of the generated ;~, carbon dioxide from the commodity chamber -to a sample cell. ~ ' r The sample cell and the reference cell are analyzed for a difference in carbon dioxide level. The presence of living insects in the commodity sample will be detected by a difference in carbon dioxide level between the sample cell and the reference cell.
Now referring to Fig. 1, a pneumatic circuit diagram is shown which illustrates the present invention.
The system comprises a container 9, filled with ambient air.
A conduit 1~ extends from container 9 and divides into two conduit paths lOa and lQb via junction 15. Conduit lOa is connected to a reference cell 11 via a filter 12. A portion of the air in container 9 is circulated through conduit lOa ~ , by means of vacuum pump 13. A flow meter 14 connected in conduit lOa controls the rate of flow through the reference ' cell 11. Conduit lOb carries a por-tion of the air in container 9 to a first valve V4. Depending upon the mb/,;- _ 7 1 23~2~
position of valve V4, the air will be directed to either valve V3 or to a second one of three commodit~ test chambers ~CTC), further designated with Roman numerals I, II and III, respectively. Valve V3 also has two positions, whereby the air may be directed to commodity chambers I
or III, respectively.
Two valves Vl and V2 are on the downstream side of the commodity test chambers CTC I and CTC III and of commodity test chamber CTC II~ respectively, and direct the circulating air from conduit lOb to a sample cell 16 via a filter 17. Filters 12 and 1? trap dust and extraneous matter from entering the cells 11 and 16~ respectively.
The pump 13 circulates air in conduit lOb as well as conduit lOa. A flow meter 18 in conduit lOb regulates the flow rate through the sample cell 16. ~ vacuum ~auge 19 is in the conduit lOb to gauge the pressure in the system and provide an indication of clogging of the filter. Air from both conduits lOa and lOb flow at the same rate in order to establish an equal background level of carbon dioxide in each cell 11 and 16, respectively. The air ~rom conduits lOa and lOb is returned to the sealed container 9 via conduit lOc. An analyzer 20 is provided to measure the ~iference in the carbon dioxide level difference in sample cell 16 and reference cell 11.
A container of carbon dioxide 21 is connected to a dosing valve V5, to introduce a given quantity or charge of carbon dioxide into conduit lOb. This charge is subsequently introduced into sample cell 16 via the valves Vl, V2, V3 and V4, so as to calibrate the system.
Fig. 2 is a pneumatic diagram for an alternate circuit arrangement for the invention. The sample cell 16 and reference cell 11 are now arranged in series with ~;~
. ~.
~l~2;3Z2~
respect to container ~ instead of in parallel (Fig. l).
The system of Fig. 2, however, operates in the exact fashion as the system in Fig. l. ~ike components have been given the same designa-tions in Fig. 2 as they had in Fig. l. Because the system is a series flow circuit, only one metering valve 18 is required to regulate the flow through the sample and reference cells.
OPERATION OF THE INVENTION
- The operation of the systems shown in Figs. 1 and 2, will be described with reference to Figs. la, lb, 2a and 2b. While these diagrams show how three commodity test chambers CTC I, CTC II, and CTC III may be opera~ed in a phased sequence, it should be appreciated that only one commodity test chamber may be used in the system consistent with the invention.
The commodity test cham~bers CTC I, CTC II, and - CTC III are initially filled with grain or another similar type commodity to be tested for infestation by live insects.
These chambers are initially open at each end, and are sealed ~rom the ambient surroundings ~hen they are connected into the system. When so connected, they form part of a closed loop defined by conduits 10b and 10c.
In the operation described in Figs. la and lb, the three filled commodity test chambers CTC I, CTC II, and CTC III are connected into the system and subsequently analyzed in a phased sequence. A typical cycle for each commodity test chamber includes an initial purging or flushing of the chamber with air from container ~. During the purging of commodity test chamber CTC I, for example, the valves Vl, V2, V3, and V4 are all deenergized to provide a fluid flow connection between commodity test chamber CTC I and the system to complete;the-~closed loop.
mh/ ~ - 9 -~232;~
At the same time, the other commodit~ test chambers CTC II
and CTC III are not fluidically connected into the system.
Rather, as illustrated in Fig. la, sample is being changed in commodity -test chamber CTC II and commodi-ty test chamber CTC III is in the incubation phase.
The purging of commodity test chamber CTC I
accomplishes two purposes: (a) it equilibrates the system by mixing any entrapped air and carbon dioxide in the test chamber with the air in the rest of the closed loop, and (b) the sample in the commodity test chamber is ~ashed clean of any residual carbon dioxide, so that the subsequent measurement of carbon dioxide in the commodity test chamber CTC I ~ill be entirely in respect of live insect respiration.
After the commodity test chamber CTC I has been purged, valves V2 and V4 are energi~ed, thus isolating commodity test chamber CTC I from the rest of the system.
During this time, commodity test chamber CTC II is fluidicall~
introduced into the closed loop and purged by the continuously circulating air, via pump 13 (see valve se~uence diagram Fi~. lb). It will be seen from Fig. lb that the commodity test chamber CTC I is now in its incubation phase. The incubation phase of commodity test chamber CTC I allows for the generation of carbon dioxide by live insects over a time period. Fig. la sho~s a three minute incubation time, but naturally, all the times of these cycles can be rearranged to suit the user of the system.
After the incubation phase of commodity -test chamber CTC I, valves Vl and V3 are energi~ed to allow air to flow through such chamber. This is the one minute analysis time designated R in Fig. la. The air flowing through commodity test chamber CTC I at this tiMe will sweep the contents, - including any g~enerated carbon dioxide, from such chamber , i ~
3:~28 to sample cell 16 for analysis. It will be noted that at this time commodity test chamber CTC II is startiny its three minute incubation phase, and is closed to the system (see Fig. lb).
If carbon dioxide has been generated by live insects in commodity test chamber CTC I, then the analyzer 20 will detect a difference between carbon dioxide levels in the reference cell 11 and the sample cell 16.
After the ana]ysis, the sample in commodity test cha~ber CTC I can be changed, as shown in Fig. la, by removing such cha~ber from the system.
The cycle for commodity test chamber CTC I can now be repeated for a new sample with the subsequent reintroduc-tion of such chamber into the system.
It will be seen by observing Figs. la and lb, that the testing of all three commodity test chambers, CTC I, CTC I~, and CTC III are phased with respect to each other, by operating valves Vl, V2, V3 and va in proper se~uence, so that a continuously phased s~stem of analysis is achieved.
Figs. 2a and 2b are identical to Fi~s. la and lb, respectively. Thus, it will also be observed that the series circuit of Fig. 2 can be operated in like fashion with the parallel circuit shown in Fig. 1.
The sequencing of the valves ma~ be controlled by a programmed computer to automate the system. Of course, other controls can be employed.
The sample cell 16, reference cell 11, and analyzer 20 are shown schema-tically as separate units, but are, in fact, packaged as a single unit. Such units are made hy Beckman (Model lR-215B) for example, and use infrared detection to distinguish the various differences ih carbon dioxide levels between the cells 11 and 16.
~L~23~Z8 .
Having thus described the invention, what is sought to be covered by Letters Patent is presented by the appended claims.
.. ,. '~
? ~ .
Claims (14)
PROPERTY OR PRIVILEGE IS CLAIMED ARE DEFINED AS FOLLOWS:
1. A system for detecting the infestation of live insects within a commodity sample comprising:
means for storing a fluid having a reference level of carbon dioxide;
at least one commodity chamber for containing a commodity sample to be tested for infestation by live insects, said live insects generating carbon dioxide to be analyzed;
a sample cell associated with said commodity chamber for receiving any generated carbon dioxide from said commodity chamber;
a reference cell associated with said sample cell for establishing a background level of carbon dioxide in said system;
first means for controlling the passage of a portion of said fluid containing said reference level of carbon dioxide from said storing means into said reference cell for establishing said background level of carbon dioxide in said reference cell;
second means defining a closed-loop comprising said storing means, said commodity chamber and said sample cell, means for circulating a portion of said fluid containing said reference level of carbon dioxide along said closed-loop, so as to transfer any carbon dioxide generated in said commodity chamber to said sample cell; and means for measuring the total carbon dioxide level in said sample cell in respect of the carbon dioxide level in said reference cell, whereby the presence of live insects in said commodity sample is detected.
means for storing a fluid having a reference level of carbon dioxide;
at least one commodity chamber for containing a commodity sample to be tested for infestation by live insects, said live insects generating carbon dioxide to be analyzed;
a sample cell associated with said commodity chamber for receiving any generated carbon dioxide from said commodity chamber;
a reference cell associated with said sample cell for establishing a background level of carbon dioxide in said system;
first means for controlling the passage of a portion of said fluid containing said reference level of carbon dioxide from said storing means into said reference cell for establishing said background level of carbon dioxide in said reference cell;
second means defining a closed-loop comprising said storing means, said commodity chamber and said sample cell, means for circulating a portion of said fluid containing said reference level of carbon dioxide along said closed-loop, so as to transfer any carbon dioxide generated in said commodity chamber to said sample cell; and means for measuring the total carbon dioxide level in said sample cell in respect of the carbon dioxide level in said reference cell, whereby the presence of live insects in said commodity sample is detected.
2. The system of claim 1, wherein said means for storing a fluid is a container for holding a given quantity of ambient air.
3. The system of claim 1, wherein said first and second means each comprises metering means for supplying said fluid at substantially equal rates through said sample cell and said reference cell.
4. The system of claim 1, further including pump means for passing said fluid along said first and second means.
5. The system of claim 4, wherein said pump means comprises a vacuum pump.
6. The system of claim 1, wherein said first and second means comprises means for defining a closed-loop between said fluid storing means and said sample cell and said reference cell so as to seal the system and supply said sample cell and said reference cell with a reference level of carbon dioxide.
7. The system of claim 6, further comprising means for introducing said reference level of carbon dioxide into said closed-loop means, to calibrate the system.
8. The system of claim 6, wherein said closed-loop means comprises means for connecting said reference cell in parallel arrangement with respect to said commodity chamber and said sample cell.
9. The system of claim 6, wherein said closed-loop means comprises means for connecting said reference cell in series arrangement with respect to said commodity chamber and said sample cell.
10. The system of claim 7, further comprising a plurality of commodity chambers and valving means for selectively connecting said plurality of commodity chambers in fluid communication with said closed-loop means to provide a phased analyzing system.
11. A method of detecting the infestation of live insects within a commodity sample, comprising the steps of:
(a) storing a given quantity of fluid containing a reference level of carbon dioxide;
(b) circulating a portion of said stored fluid through a reference cell in a controlled manner to establish a controlled level of carbon dioxide in said reference cell;
(c) incubating a commodity sample disposed within a commodity chamber to generate a sample level of carbon dioxide;
(d) circulating a portion of said stored fluid in a controlled manner through said commodity chamber and said sample cell and then to said commodity chamber, in closed-loop fashion, so as to transfer any carbon dioxide generated in said commodity chamber to said sample cell; and (e) measuring any difference between carbon dioxide levels in said sample cell and said reference cell, respectively, to detect the presence of any living insects in the commodity sample.
(a) storing a given quantity of fluid containing a reference level of carbon dioxide;
(b) circulating a portion of said stored fluid through a reference cell in a controlled manner to establish a controlled level of carbon dioxide in said reference cell;
(c) incubating a commodity sample disposed within a commodity chamber to generate a sample level of carbon dioxide;
(d) circulating a portion of said stored fluid in a controlled manner through said commodity chamber and said sample cell and then to said commodity chamber, in closed-loop fashion, so as to transfer any carbon dioxide generated in said commodity chamber to said sample cell; and (e) measuring any difference between carbon dioxide levels in said sample cell and said reference cell, respectively, to detect the presence of any living insects in the commodity sample.
12. The method of claim 11, further comprising the step of:
flushing said commodity chamber prior to step (c).
flushing said commodity chamber prior to step (c).
13. The method of claim 11, further comprising the step of:
prior to the incubating step (c), sealing said commodity chamber and said sample cell and said reference cell to define a closed-loop.
prior to the incubating step (c), sealing said commodity chamber and said sample cell and said reference cell to define a closed-loop.
14. The method of claim 13, further comprising the step of:
calibrating the system by introducing a given quantity of carbon dioxide to said closed-loop.
calibrating the system by introducing a given quantity of carbon dioxide to said closed-loop.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US910,727 | 1978-05-30 | ||
| US05/910,727 US4206353A (en) | 1978-05-30 | 1978-05-30 | Analyzing system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1123228A true CA1123228A (en) | 1982-05-11 |
Family
ID=25429247
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA318,354A Expired CA1123228A (en) | 1978-05-30 | 1978-12-21 | Detection of live insects in a commmodity sample |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US4206353A (en) |
| JP (1) | JPS54156695A (en) |
| AU (1) | AU4574379A (en) |
| CA (1) | CA1123228A (en) |
| DE (1) | DE2921662A1 (en) |
| DK (1) | DK221379A (en) |
| FR (1) | FR2427602A1 (en) |
| GB (1) | GB2022245A (en) |
| SE (1) | SE7813126L (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4947339A (en) * | 1988-12-01 | 1990-08-07 | Jan Czekajewski | Method and apparatus for measuring respiration, oxidation and similar interacting between a sample and a selected component of a fluid medium |
| US6255652B1 (en) | 1999-05-14 | 2001-07-03 | William T. Moyer | Method and apparatus for detecting insect infestation in enclosed areas |
| US7234269B2 (en) * | 2004-06-25 | 2007-06-26 | S.C. Johnson & Son, Inc. | Animal test chamber |
| JP2007108151A (en) * | 2005-10-14 | 2007-04-26 | Junzo Umemura | Fourier transform infrared spectrophotometer without spectrum noise by steam or carbon dioxide gas |
| US20090072148A1 (en) * | 2007-09-19 | 2009-03-19 | Moyer William T | Method and apparatus for detecting wood eating insect infestation in enclosed shipping containers or in trees |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3407124A (en) * | 1964-10-30 | 1968-10-22 | Universal Oil Prod Co | Method of analyzing trace water |
| US3836255A (en) * | 1972-04-06 | 1974-09-17 | M Schuman | Spectrometric substance analyzer employing temperature modulation |
| US3963927A (en) * | 1975-06-18 | 1976-06-15 | The United States Of America As Represented By The Secretary Of Agriculture | Detection of hidden insects |
-
1978
- 1978-05-30 US US05/910,727 patent/US4206353A/en not_active Expired - Lifetime
- 1978-12-20 SE SE7813126A patent/SE7813126L/en not_active Application Discontinuation
- 1978-12-21 CA CA318,354A patent/CA1123228A/en not_active Expired
-
1979
- 1979-02-14 JP JP1511079A patent/JPS54156695A/en active Pending
- 1979-02-28 GB GB7907074A patent/GB2022245A/en active Pending
- 1979-04-03 AU AU45743/79A patent/AU4574379A/en not_active Abandoned
- 1979-05-29 DK DK221379A patent/DK221379A/en unknown
- 1979-05-29 FR FR7913566A patent/FR2427602A1/en not_active Withdrawn
- 1979-05-29 DE DE19792921662 patent/DE2921662A1/en not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| US4206353A (en) | 1980-06-03 |
| DK221379A (en) | 1979-12-01 |
| SE7813126L (en) | 1979-12-01 |
| JPS54156695A (en) | 1979-12-10 |
| DE2921662A1 (en) | 1980-01-24 |
| AU4574379A (en) | 1979-12-06 |
| FR2427602A1 (en) | 1979-12-28 |
| GB2022245A (en) | 1979-12-12 |
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