CA1120310A - Powdered compositions and methods for the manufacture of acidified milk products - Google Patents

Abstract

ABSTRACT OF THE DISCLOSURE
The invention provides a powdered composition suitable for mixing with milk to obtain an acidified milk product comprising, per liter liquid, about 10 to about 50 g of an acidogen and about 2.5 to about 15 g of a mild acidic buffer as well as providing a powdered composition suitable for mixing with water to obtain an acidified milk product comprising per liter liquid about 10 to about 50 g of an acidogen, about 2.5 to 15 g of a mild acidic buffer and about 90 to about 240 g dry milk.

Description

llZ0310 1 The present invention relates to powdered compositions and methods for the manufacture of acidified milk products therewith. More particularly the pres~ent invention relates to a powdered composition suitable for mixing with milk or water to form an acidified milk product having the delicate texture normally associated with natural yogurt and other sour milk products.
Yogurt, is a milk product having a gelled texture and biologically acidified by means of Lactobaclllus bulgaricus and Streptococcus thermophilus, the pH of which are about 4.1 to 4.6.
The basic theory behind the use of yogurt has been to implant a beneficial culture of Baci~us bulgaricus-Lactobacillus acidophilus in milk, to allow this mixture to incubate until these particular species of bacilli sour the milk and then chill the product to prevent further growth.
The use of yogurt has been quite popular and common as it forms not only a healthful but a pleasant food substance whlch may be enjoyed for its flavor, its health qualities or its reputed regulatory effect on the bowel movement.
When yogurt in its usual form is dehydrated it cannot be reconstituted into the smooth, creamy, pleasant tasting product by the simple addltion of water, as might be expected.
It has been found that during drying the characteristic texture of the yogurt is lost, the yogurt reconstituted from yogurt powder with water remains flu~d and the coagulated phase tends to separate rapidly from the aqueous phase.

1 Previous attempts to manufacture yogurt in powder form have resulted ;n a culture which could be implanted in milk and allowed to yrow quickly and vigorously in a type of "do-it-yourself" yogurt rnanufacture. These attempts, however, at rapid manufacture of yogurt ha~e resulted in poorly con-trolled growth conditions which yielded an inferior quality and taste.
The method described in US Patent 3 793 465 where a milk product is biologically acidified to pH of 4.2 and combined with a non-acidified milk product and acid compound with control release ~ yield a yogurt-like product has also proven to be too expensive and not practical.
In US Patent 3 080 236 there is described an instant yogurt product adapted when mixed with water to form a synthetic yogurt composition, said product comprising dried yogurt culture, a water soluble dried milk, an edible vegetable oil and a water-soluble dried starch, however, as will be realized said patent is also based on a powdered yogurt culture, which is dried in a vacuum drier to overcome some of the prior dehydrating and reconstltuting difficultles.
In US Patent 3 917 875 there is described a powdered dessert composition suitable for mixing with ~ilk to obtain an acidic dessert consisting essentially of 5~ to 20.0 per-cent of a cold water-soluble gelatin, 0.2 to 5.0 percent of a stabilizer, S.0 to 25.0% of a pregelatinized starch, 2.0 to 7.0 percent of a food acid, 40.0 to 75.0 percent of a sweetening agent, from 0.0 to 25.0 percent yogurt powder, from 0.0 to 10.0 percent of an emulsifier and fro~ 0.0 to an effective amount of color and flavoring ingredients, all 30 percentage being by weight of the powdered dessert composition, which patent does not rely on dry yogurt powder culture and instead uses a natural acid to achieve the acidifying effect.

llZ0310 1 It has been found however, that the direct intro-duction of d~ acid i~ powder form does not produce a homogenous gradual development of an acidic solution which is essential to the delicate texture associated with natural yogurt and sour milk products.
In contradistinction to said prior art processes and products and according to the present invention it has now been found that it is posslble to achieve the product texture desired without relying on bacterial action of a dried culture.
Thus the present invention provides a powdered composition suitable for mixing with milk to obtain an milk acidified milk product comprising, per liter/liquid, about 10 to about 50 g of an acidogen and about 2.5 to about 20 g of a mild acidic buffer, as well as providing a powdered composition suitable for mixing with water to obtain an acidified milk product comprising per liter liquid about 10 to about 50 g of an acidogen, about 2.5 to 20 9 of mild acidic buffer and about 90 to about 240 g dry milk.
As is known and as used herein the term acidogen refers to a composition adapted to undergo spontaneous hydrolysis in aqueous medium. The acidogen used is pre-ferably selected from the group consisting of lactones and anhydrides such as glucono delta lactone, lactide, propionic anhydride, propionic isovaleric mixed anhydride, acetic-isovaleric mixed anhydride, acetylvaleryl mixed anhydride,acetyl butyryl mixed anhydride or from an ester-esterase enzyme pair such as the esters of citric, tartaric, acetic, malic, lactic, fumaric, maleic and adipic acid in combination with enzyme esterase.

~1~0~

1 As will be realized in essence the present invention is based on the interaction of the acidog~n, the ~uffer and milk in aqueous medium and thus the acidogen and buffer can be packaged together in dry for~ for addition to milk or can be packaged together with dried milk for later addition to water.
The present invention is thus directed toward an acidifying dry mix which can be reconstituted quickly and simply with cold milk or water without curdling of the milk proteins, to produce a tangy or yogurt-like dessert which will not deteriorate on standing for relatively long periods of time after its preparation.
One of the advantages of the product of the present invention is that the acidogen turns to acid gradually by hydrolysis with aqueous medium and thereby simulates the action of bacterial fermentation of lactose which also is a gradual homogeneous acidifying process.
The products of the present invention also have the following advantages:
a) The lnnovative product in powder mix form can be prepared to form a yogurt-like composition without the use of household machinery, without kitchen utensils and without a blender. All that is required is a spoon for stirring and a container to hold the contents. The yogurt-like composition is therefore freshly prepared at all times and prepared in the desired quantities.
The powder is mixed with milk or water and forms into a yogu~t-like composition within 3Q to 50 minutes. In comparison, the natural yogurt requires 14 hours of processingsbefore it is ready for consumption;

ilZO~10 1 b) The innovative product in powder mix form packaged in plastic or aluminum foil can be stored a1most any-where in the home, at the place of work, in an office, in d vehicle, in a boat or in a plane, without the need of refrigeration. Like a teabag it can be carried in the purse, in the shirt pocket, or in the wallet. It can be prepared as a luncheon dessert, a coffee break snack or a mid-night delight. In cornparison, the natural product re4uires refrigeration and, must be consumed within a limited period, otherwise it spoils.
It is too bulky to be carried and cannot be transported for distances without refrigeration;
c) The innovative product when prepared as a yogurt-like composition contains all the vitamins and proteins of ordinary milk. In comparison, the natural yogurt product which is formed by bacterial fermentation lacks some of the vitamins which have been destroyed or depleted as a result of the bacterial action and has less protein;
d) As compared with an equal supply of a natural yogurt, the composition of the innovative product costs no more than one-half of the natural commercially sold product;
e) The instant yogurt in powder mix form packaged in plastic or cellaphane or aluminum foil containers can last six or more months without the need of refrige-ration, without the fear of spoilage and without the danger of contamination. In cornparison, the natural yogurt product which is formed by bacterial fermenta-tion requires refrigeration against spoilage andcontamination.

l~Z~310 1 f) The instant yogurt can be flavored homogeneously and completely with any flavor. Additional ingredients such as sugar or acid do not prove detrimental to the product.
In comparison, the natural yogurt product which is formed by bacterial fermentation has a limited selection and choice of flavors. Too much sugar tends to inhibit or interfere with the bacterial fermentation. Also~ the natural yogurt product cannot be completely and homogene-ously flavored throughout the entire compositioni 9) The innovative product, does not lose any of its body texture when it is prepared, frozen and subsequently thawed out;
h) The instant yogurt can be used safely and without any negative reaction by individuals allergic to products pro-duced by means of biological fermentation and who are not allergic to milk products;
i) The instant yogurt product in powder mix form can also be mixed with "half and half" cream to produce an instant sour cream composition;
j) The instant yogurt product in powder mix form can be supplemented with a protein and a fat substance derived from plant sources in addition to the sodium caseinate, thereby producing a non-dairy yogurt-like product with reduced cholesterol;
k) The instant yogurt product in powder mix form can be processed to produce a whipped ice cream or a frozen yogurt composition; and 1) The mix of the present invention can be incorporated in milk based desserts without coagulation of the milk 1~0310 1 protein and allows the formulation of fruit-flavored desserts which simulate the flavor and texture of of flavored yogurt including the formulation/whipped frozen yogurt and yogurt fruit flavored ice cream.
The present invention also provides a method for the manufacture of an acidified milk product from a dry powdered mix composition comprising combining with milk a dry mix containing per liter liquid, about 10 to about 50 9 of an acidogen and about 2.5 to about 2~ 9 of a mild acidic buffer, gently stirring for about 1 to 4 minutes and allowing the product to stand for about 20 to about 60 minutes and a method for the manufacture of an acidified milk product from a dry powdered mix compo-sition comprising combining with water a dry mix containing per liter liquid, about 10 to about 50 9 of an acidogen, about 2.5 to about 20 9 of a mild acidic buffer and about 90 to about 240 9 dry milk, stirring for about 1 to 4 minutes and allowing the product to stand for about 20 to about 60 minutes.
While the three above enumerated ingredients are in fact sufficient for producing the desired product other ingredients can preferably be added to improve or modify taste and texture.
Thus the above powdered mix composition can further comprise one or more milk clotting enzymes, emulsifiers, stabilizers, taste additives, flavorings or sugars wherein all of said components are in anhydrous fine powder form.
Such add~tional ingredients and their function are described and exemplified hereinafter as follows:

l~Z03~L~
1 a) Stabilizers and thickeners such as pregelatized starch and gums like gum arabic, guar gum, irish moss, sodium alginate and carboxymethyl cellulose or a combi-nation thereof. The combination of the ~abilizers and thickeners affords increased stability to the finished yogurt, permits rapid hydration, quick setting of the jellying material, increases the viscosity and provides a better body and texture of the finished product. Range in the formula: 1 9 - 25 9 / liter liquid.
b) An Emulsifier such as tween 60 and lecitin. The emulsifier appears to aid in the ~hydration, to contribute smoothness and to suspend insoluble particles in the mixture. Also it stabilizes the particles of the lipids in the yogurt. The emulsifier could be coated on the granules of either the sugar or any other part of the powder mix. Range 1 9 - 5 9 /liter.
c) Su~ar - Sucrose is the preferred sugar to be used as a sweetener although other sugars such as dextrose, corn syrup solids, lactose, etc. may of course, be used. Such sugars may be employed singly or in combination. Moreover, artificial sweeteners such as, for example, edible saccharin salts, dipeptide salts and the llke may be included in the dry mix to replace all or part of the sugar. Range: 100 y - 200 y sugars / l~ter liquid, or 0.1 - 0.5 g artificial sweetener/liter liquid.
d) A clotting enzymatic agent l~ke pepsin derived from -cow, turkey or chicken stomachs, or mucor pussilus enzyme, Aspergillus protease, penicillium protease, etc. One of these agents or a combination of two or more of them would jellify the casein of the milk within the desired period of time, even in a low pH.
Range: 100 ~9 - 10 mg/liter liquid.

llZ~310 1 e) Flavors - The l!li,~ cdn alsQ contain flavors such as strawber~^y, pineapp~e, apricot, lemon, etc. in amounts up to about 0.5% by weight ~ provide a flavor simulative of tan~y fruit yogurt. Range: 0.05g - 5g/liter.
If desired the mix can also contain:
f) Yogurt flavor in powder form or ~n liquid.
Range: 0.2g - 2.5g/liter and/or g) Dry starter cells such as lactobacillus bulgaricus, streptococcus thermophillus or lactobacillus acidophillus.
This ingredient is favorable for natural aroma and taste in the finished yogurt; the alive cells may grow in the milk either during the waiting time for clotting or there-after; and there are some opinions that such cells are important for health because they produce vitamins and effect is easing the bowel movement.
With regard to the main essential ingredients the function,of the acidogen is to gradually and homogenously acidify the milk to the desired pH and to simultaneously act as a iellifying agent of the casein as the pH is lowered slowly below the iso-electric point of the casein and the function of the buffer is to reduce the time duration for jellying by reducing the pH close ~ the point of jellying which is approximately pH 4.8.
Without the buffer the jellying time is too long,and not practical because of the slow hydrolysis of the acidogen. Using a mild acidic buffer allows the acidogen to reduce the pH
a short range of ~.3-O.g pH units only, which is done within 10-30 minutes.

llZQ310 l Employing a buffer also controls the decreasing of the pH, maintains it around pH 4.0 and does not allow it to decrease too much down thereby preventing the pro-duct from turning too sour.
Dry "milk" can also be added to the mix adapted for addition to milk in order to increase the casein content of the mix which clots by the enzyme and iellifies by the acid. High levels of casein provides a better body and texture. Non fat dry milk also increases the nutritious value of the product as it adds more proteins and vitamins.
The dry milk could be in the form of skim, whole or fermented milk. Also it could be substituted by soybean protein or any other soluble protein, or a combination.
Range in the formula is lOg - 120g/liter liquid.
While the invention will now be described in connection with certain preferred embodiments in the following examples it will be understood that it is not intended to limit the invention to these particular embodiments. On the contrary it is intended to cover all alternatives, modifications and equivalents as may be included within the scope of the invention as defined by the appended claims. Thus, the following examples which include preferred embodiments w~ll serve to illustrate the practice of this invention, 1t being understood that the particulars shown are by way of example and for purposes of tllustrative discussion of preferred embodiments of the present.invention only and are presented in the cause of providing what is believed to be the most useful and readtly understood description of formulation procedures as well as of the principles and conceptual aspects bf the invention.

llZ~310 1 ExP~1PLE 1 - Unflavored sour ~ogurt _ Glucono delta lactone 9 g Calciu~ phosphate monobasic monohydrate 1.5 g Instant dry milk 40 g The above dry powder composition was mixed with 200 ml tap water for a~out 2 minutes and allowed to stand for 1/2 to 1 hr. upon which a uniformly consistant nonfermented yogurt-like product was formed.
EXAMPLE 2 - Plain yogurt Mix lO Glucono delta lactone 10 g Calcium phosphate monobasic monohydrate 1~5 g Monosodium citrate Q~5 g Instant non-fat dry milk 20 g Precooked starch Redisol* 88 ~Staley~1.0 g Sodium alginate Keltone* (Kelco) 0~5 g Yogurt flavor 0.25g Tween* 60 ~coated on glucono delta lactone) l~Q g The ingredients are dissolved in 200 ml whole milk (3.8% fat) mixed well for three minutes and allowed to stand for 45 min. at room temperature~
EXAMPLE 3 - Strawberry yogurt Mix Glucone delta lactone 10 g Calcium phosphate monobasic monohydrate 2 g Monosodium citrate 0,5 g Sugar 25 g Precooked starch Redisol* 88 ~Staley)1 g Sodium alginate Keltone* (Kelco) 0.5 g Freeze dried plain yogurt 1.0 g Yogurt flavor 0.15g 30 Strawberry flavor 0~5 g Red color 0~1 g Tween* 60 coated on the sugar 1,O g `v, ~ ;
~- *Trade Marks -12-11;~03~0 1 The mix is dissol~ed in 200 ml milk ~2.8~ fat) mixed well for four minutes and allowed to stand at room temperature for 45 minutes.
EXAMPLE 4 - Acidified Buttermilk Proprionic isovaleric anhydride 3.8 9 Calcium phosphate monobasic monohydrate 3.3 9 Citric acid 0.25 9 Precooked starch 2 9 Instant dry milk 5 9 Buttermilk powder 2.5 g The above composition was mixed thoroughly for 2-3 minutes with 200 ml milk and allowed to stand for 1-2 hrs to form an acidified buttermilk product.
EXAMPLE 5 - Leben mix Lactide 4 9 Calcium phosphate monobasic monohydrate 3 9 Citric acid 0.25 9 precooked starch 1.5 9 Dry skim milk 5 9 The above dry mix was dissolved in 200 ml milk, stirred for 1 minute and allowed to stand 45 minutes to form a leben-like acidified milk product.
EXAMPLE 6 - Whipped cold pineapple yogurt Isopropyl lactate 4.5 9 Esterase 5 mg Monosodium tartarate 1 9 Susar 30 9 Sodium caseinate 2.5 9 Egg albumin 2 9 Gelatine hydrolizate ~partially) 1.5 9 Pineapple flavor 0.3 9 Yogurt flavor 0.1 9 llZ~310 1 The dry mix was dissolved in 200 ml milk at room temperature (having a 2.8% fat content) was mixed with an electric mixer for 2 minutes and allowed to stand for 1 to 1.5 hrs and then refrigerated to cool and whipped to proper consistancy with an electric mixer.
EXAMPLE 7 - Sour Cream -Hog. Pepsin 200 ~g Glucono delta lactone 10 9 Calcium phosphate monobasic monohydrate 2 9 Instant dry whole milk 20 9 Precooked starch 1 g Sodium alginate 0.5 9 Guar Gu~ 0.25 9 Yogurt flavor 0-3 9 B Tween~60 (coated on glucon delta lactone) The powder is dissolved ~n 200 ml whole milk (3.8%
fat), and let stand for 40 m~n. at room temperature.
EXAMPLE 8 - Coffee Yogurt_Mix Glucono delta lactone 8 9 Instant dry whole milk 5 9 Calcium phosphate mono basic monohydrate 3 9 Precooked starch (National) 0.75 9 Sugar 25 9 Ground instant coffee (Taster Cho~ce) 2.5 9 Freeze dried Leben 0.5 9 The mlx powder is dissolved in 200 ml milk (1%
fat), mixed and allowed to stand for 30 minutes.
T~ade 1'1~k ~lZ1~310 1 EXAMPLE 9 - Yo~urt Mix for Whi~ped Frozen Yo~urt Glucone delta lac~one 100 9 Disodium citrate 12.5 9 Citric Acid 12.5 g Freeze-dried yogurt 40 g Precooked starch 10 g Sodium alginate 21 g Skim milk powder 75 9 Strawberry flavor 10 9 Sugar 600 9 Red color 2.5 The mixture is dissolved in 4 liter pasteurized milk (2.8% fat), allowed to stand for 60 minutes at room temperature and then processed in a soft ice cream maker.
EXAMPLE 10 - Yogurt Mix for Ice Cream Glucone delta lactone 80 9 Disodium citrate 10 9 Citric acid 10 9 Precooked starch 10 g 20 B Dry yogurt powder (Bevita S.A.) 23 9 The powder is mixed with 4 liter of fruit flavored liquid ice cream mix and allowed to stand for 90 minutes at room temperature.

It will be evident to those. skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments and that the present invention may be embodied in other specific forms without departing from the essential attributes thereof, and it is therefore desired that the present embodiments and ~ 1~ k ~,i2~.~`10 1 examples be considered in all respec~s as illustrative and not restrictive, reference being made ~ the appended claims, rather than to the foregoing Jescription, and all changes which come with the meaning and range of equiva-lency of the claims are therefore intended to be embraced therein.

Claims (9)

The embodiments of the invention in which an exclusive property or privilege is claimed are defined as follows:

1. A powdered composition adapted for mixing with milk to produce an acidified milk product having the texture of sour milk products without curdling of milk proteins, said composition com-prising, for each liter of milk with which it is to be mixed, a) about 10 to about 50 g. of an acidogen, said acidogen being characterized by undergoing spontaneous hydrolysis in aqueous medium and turning to acid gradually so as to homogene-ously acidify the milk thereby simulating the action of bacterial fermentation of lactose, and simultaneously acting as a jelly-ifying agent as the pH is lowered and, b) about 2.5 to about 20 g of a mild acidic buffer, said buffer being effective to control the decreasing pH when mixed with the milk thereby preventing the milk product from turning too sour, and said buffer being further characterized in that upon mixing with said milk, said buffer reduces the time duration of jellying of the resulting mixture to about 10 to 30 minutes by reducing the pH of the mixture close to the point of jellying whereafter said acidogen need further reduce the pH only a short range of about 0.3 to about 0.9 pH units.

2. A powdered composition according to claim 1 wherein said acidogen is selected from the group consisting of lactones, anhydrides and ester-esterase enzyme pairs.

3. A powdered composition according to claim 1 wherein said acidogen is selected from the group consisting of glucono delta lactone, lactide, propionic anhydride, propionic-isovaleric mixed anhydride, acetic-isovaleric mixed anhydride, aceticvaleric mixed anhydride and acetic butyric mixed anhydride.

4. A powdered composition according to claim 1 wherein said acidogen is selected from the group consisting of esters of citric, tartaric, acetic, malic, lactic, fumaric, maleic and adipic acid in combination with enzyme esterase.

5. A powdered composition according to claim 1 wherein said buffer is selected from the group consisting of sodium citrate monobasic, sodium citrate dibasic, calcium phosphate monobasic, sodium pyosphate monobasic, sodium tartarate monobasic, sodium malate monobasic or a combination thereof.

6. A powdered composition adapted for mixing with water to produce an acidified milk product having the texture of sour milk products without curdling of milk proteins, said composition com-prising, for each liter of water with which it is to be mixed, a) about 90 to about 240 g of dry milk, b) about 10 to about 50 g of an acidogen, said acidogen being characterized by undergoing spontaneous hydrolysis in aqueous medium and turning to acid gradually so as to homo-geneously acidify the milk thereby simulating the action of bacterial fermentation of lactose, and simultaneously acting as a jellyifying agent as the pH is lowered and, c) about 2.5 to about 20 g of a mild acidic buffer, said buffer being effective to control the decreasing pH when mixed with the water thereby preventing the milk product from turning too sour, and said buffer being further characterized in that upon mixing with said water, said buffer reduces the time duration of jellying of the resulting mixture to about 10 to 30 minutes by reducing the pH of the mixture close to the point of jellying whereafter said acidogen need further reduce the pH
only a short range of about 0.3 to about 0.9 pH units.

7. A synthetic yogurt mix according to claim 6 further comprising one or more milk clotting enzymes, emulsifiers, stabilizers, taste additives, flavorings or sugars wherein all of said components are in anhydrous fine powder form and said mix is adapted upon addition to water with gentle stirring for about 1 to 4 minutes to produce a yogurt-like product after standing for about 20 to about 60 minutes.

8. A method for the manufacture of an acidified milk pro-duct from a dry powdered mix composition comprising combining with milk a dry mix containing per liter liquid, about 10 to about 50 g of an acidogen and about 2 5 to about 20 g of a mild acidic buffer, gently stirring for about 1 to 4 minutes and allowing the product to stand for about 20 to about 60 minutes,

9. A method for the manufacture of an acidified milk pro-duct from a dry powdered mix composition comprising combining with water a dry mix containing per liter liquid, about 10 to about 50 g of an acidogen, about 2.5 to about 20 g of a mild acidic buffer and about 90 to about 240 g dry milk stirring for about 1 to 4 minutes and allowing the product to stand for about 20 to about 60 minutes.