CA1109785A - Microbial insecticides - Google Patents
Microbial insecticidesInfo
- Publication number
- CA1109785A CA1109785A CA311,958A CA311958A CA1109785A CA 1109785 A CA1109785 A CA 1109785A CA 311958 A CA311958 A CA 311958A CA 1109785 A CA1109785 A CA 1109785A
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- Prior art keywords
- microbead
- microbeads
- nucleic acid
- chemical products
- embedded
- Prior art date
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/26—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests in coated particulate form
- A01N25/28—Microcapsules or nanocapsules
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
- A01N63/23—B. thuringiensis
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/40—Viruses, e.g. bacteriophages
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Virology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
ABSTRACT:
An improved insecticidal composition is disclosed comprising a microbial agent of viral or bacterial origin embedded in a microbead, wherein the microbead contains sufficient radiation-absorbing material to effectively shield the agent from sunlight-induced inactivation. Typically the microbead is comprised of a nucleic acid and a protein-containing material, and is stabilized by chemical crosslinking.
A method for preparing a microbial insecticide composition is also disclosed comprising: mixing a buffered aqueous solution containing a microbial insect pathogen with an aqueous solution containing a nucleic acid, and mixing the said mixture with an aqueous solution containing a proteinaceous material, thereby spontaneously forming microbeads having the pathogen embedded therein. Typically the microbeads are subsequently stabilized by treatment with chemical crosslinking agents, and typically, prior to the two mixing steps, the microbial insect pathogen is cleaned by washing and its surface charge is modified by the addition of a protein-modifying agent adapted to provide a more uniform surface charge.
An improved insecticidal composition is disclosed comprising a microbial agent of viral or bacterial origin embedded in a microbead, wherein the microbead contains sufficient radiation-absorbing material to effectively shield the agent from sunlight-induced inactivation. Typically the microbead is comprised of a nucleic acid and a protein-containing material, and is stabilized by chemical crosslinking.
A method for preparing a microbial insecticide composition is also disclosed comprising: mixing a buffered aqueous solution containing a microbial insect pathogen with an aqueous solution containing a nucleic acid, and mixing the said mixture with an aqueous solution containing a proteinaceous material, thereby spontaneously forming microbeads having the pathogen embedded therein. Typically the microbeads are subsequently stabilized by treatment with chemical crosslinking agents, and typically, prior to the two mixing steps, the microbial insect pathogen is cleaned by washing and its surface charge is modified by the addition of a protein-modifying agent adapted to provide a more uniform surface charge.
Description
` ` ~11~)978S
`
MICROBI`AL INSECTICIDES
;- Technical Field ; This invention relates to microbial insecticides. More particularly, the invention relates to a novel microbial insecticide composition 5 and to the production and utilization thereof.
Background Art Microbial insecticides of viral or bacterial origin offer signi-ficant advantages over conventional chemical insecticides. Microbial insect pathogens are lO generally nontoxic and harmless to other forms of life.
In addition, microbial insecticides demonstrate a relatively high degree of specificity, and hence do not endanger beneficial insects. Moreover, a suscep-tible insect host is quite slow to develop resistance 15 to microbial pathogens. Microbial insecticides may be used in relati~ely low dosages, may be effectively applied as dusts or sprays, and may be used in combination with chemical insecticides.
For example, Bacillus thuringiensis (B.t.), 20 a spore-forming bacterium, is well-known as a micro-bial insect pathogen useful against numerous leaf-chewing insects in their larval stages, including, for example, alfalfa caterpillars, tomato hornworms, ., ~
~1~)9'785 tobacco hornworms, cabbage loopers, cabbage web worms, army worms, gypsy moths, walnut caterpillars, diamond-back moths, cosmopolitan green beetles, European corn borers, and other members of the order Lepidoptera.
Unfortunately, the effectiveness and usefulness in the field of many microbial insect pathogens as insecticides is severly limited by their extreme sensitivity to sunlight. It is known that nonionizing radiation having a high photon energy 10 (e.g. ultraviolet rays o~ sunlight) exerts an inactivating effect on various microbial insect pathogens. See, e.g., "Photoprotection Against Inactivation of Bacillus thuringiensis Spores by Ultra~iolet Rays," Aloysius Krieg, Journal of 15 Invertebrate Patholo~y, Vol. 25, pp. 267-268 (1975).
For example, it is known that ultraviolet (W) rays with a wavelength of 253.7 nm induce a marked, extra-- ordinary inactivation of Bacillus thurin~iensis (B.t.) spores, so that they are unable to germinate and grow 20 out. A dosage of 18 m W sec/cm2 of such UV radiation will inactivate 99.9% of the B.t. spores. ~e have determined that the half life of B.t. subjected to W
radiation of sunlight in the field is approximately six minutes. ~i~ewise, it has been determined by 25 others that the half lives of certain occluded viruses subjected to W radiation of sunlight in the field is - one and one-half hours. Thus, the effectiveness of a typical spray application of such microbial insecticides is rapidly lost in the field.
Since nucleic acids show a maximum of - extinction near a wavelength of 260 nm, it is believed that the UV induced death of B.t. at 253.7 Nm, and of certain occluded viruses at comparable wavelengths, may be caused by a photoreaction of the genetic 35 material, especially DNA. Thus, it has been suggested, li`()9785 ibid., at p. 267, that B.t. spores could be protected from inactivation by UV
radiation by physically mixing the B.t. spores with DNA, or a comparable nucleic acid which would absorb the UV rays. Such a comparable nucleic acid would be RNA, Ribonucleic Acidl which has a maximum of extinction near 260 nm.
However, this technique proved to be ineffective.
Disclosure of Invention In one aspect, the present invention provides an insecticidal composi-tion comprising a microbial agent in combination with at least one of its bio-logically active chemical products embedded in a coacervate microbead having an effective diameter within the range of from about lO to about 150 microns which is comprised of a nucleic acid and a proteinaceous material admixed in a nucleicacid to proteinaceous material ratio in the range of from about 1:5 to about 5:1.
In another aspect the invention provides a method of embedding micro-organisms and their biologically active chemical products in microbeads, comprising:
(a) mixing a buffered aqueous solution containing microorganisms and - their biologically active chemical products with an aqueous solution containing - a nucleic acid; and (b) mixing the said mixture with an aqueous solution containing a proteinaceous material, thereby spontaneously forming microbeads having the microorganisms and chemical products embedded therein.
In the present invention, materials capable of absorbing radiation which would otherwise inactivate the microbial insect pathogen of concern are incorporated in a protective coating which surrounds the pathogen in the form of a microbead. These materials serve to intercept and block the harmful radia-tion before it reaches the light-sensitive material of the insect pathogen. In a typical embodiment of the invention the material selected to intercept the harmful radiation comprises a nucleic acid; and in a preferred embodiment the iL1~9785 material comprises R~A. Various other materials and combinations of materials may be used in combination witll tlle nucleic acid depending on the specific in-sect pathogen to be protected and the microbead coating system to be used;
including, but not limited to the following materials: protamine, cytochrome c, soy protein, hemoglobin, gelatin, etc. In general, any protein can be used if the conditions are adjusted as to facilitate formation of the microbeads. Such conditions may include charge modification techniques, adjustments in pH, component concentrations, etc.
~he microbeads in which the microbial insect pathogen are embedded may be advantageously produced using any known technique for forming what have been called coacervate droplets or microbeads. One such - 3a -~1~978S
technique was developed in conjunction with the study of the origin of life on earth, and has been used to construct precellular models. See, for example, Evreinova, et al., Journal of Colloid and Interface 5 Science, Vol. 36, No. 1 (1971).
Brief Description of Drawings Fig. 1 is a graph showing the optical density (i.e., absorption) over the solar UV range of typical microbeads suitable for use in the present 10 invention.
Figs. 2-6 are graphs showing the comparative experimental data from Examples 1, 2, 4, 5, and 6, below, respectively. In Figs. 2-4 the number of viable spores, extrapolated to 1 ml of original 15 sample, is shown as a function of the length of time of exposure to the W radiation. In Figs. 5 and 6 the percentage of B.t. remaining as survivors is shown as a function of the exposure time.
Best Mode for Carrying Out the Invention In one preferred em~odiment of the invention the microbeads axe produced using the above-mentioned technique as follows. An aqueous solution containing a nucleic acid such as, for example, RNA, and a buffering agent such as, for example, phosphate, 25 designed to maintain a pH at the position which optimizes the charge on the RNA, the protein and the microbe (where the microbe is sensitive to pH, this should be taken into account as well), is mixed with an aqueous solution containing an appropriate protein 30 material, such as, for example, protamine, gelatin, soy isolate, hemoglobin, etc. or synthetic amino acid polymers. Protein-nucleic acid microbeads which are 11~9785 essentially solid and roughly spherical form spontaneously upon the mixing of these two solutions.
It is important to note that while all of the above-named protein materials, and others, can be 5 used satisfactorily in forming the microbeads, care must be taken to maintain the pH of the mixture of solutions on the acid side of the isoelectric point of the particular protein being used since this is required for formation of the microbeads. If a 10 protein is used that is insoluble at a given pH, the p~ will have to be put in a range tn which the protein is soluble, or other steps will have to be taken to make the protein soluble. These steps could include partial degradation, charge modification or adding 15 other components in the buffers (e.g. detergents, alcohols, surfactants, etc.). If foaming of one or more components is a problem, simethicone type agents (U.S. patent No. 2,441,098) can be included. When RNA
is being used as the nucleic acid, the pH of the mix-20 ture of solutions must be maintained at or above about4.3 to prevent the RNA from precipitating out of the microbead, with the protein necessarily leaving the microbead and going back into solution.
As can be seen, in the above-described method 25 for forming microbeads (i.e., coacervate droplets) the material to be utilized to intercept and absorb the harmful radiation, i.e., the nucleic acid, is incor-porated directly in the microbead structure, thereby producing a highly protective coating.
The bimolecular structure of the microbeads creates a thermodynamically stable cooperation between the components, so that even without subsequent chemical crosslinking, as described below, the components will not individually diffuse ou~ of the 35 microbeads. The bimolecular structure also causes the 11~9785 microbeads to be highly charged. These charges should aid the microbeads in sticking to plant surfaces.
These charges can be controlled by selecting the appropriate protein to be used in forming the 5 microbead.
The size of the microbeads can be controlled by controlling the concentration of the nucleic acid and the protein in the formation vessel. For example, 100~ beads can be made by mixing 5~ RNA with 10%
10 protamine sulfate. Beads will form and settle to the bottom of the vessel. Most of these will be in the 100~ range. While a relatively wide range of nucleic acid and protein concentrations can be used to make these microbeads generally, in preparing microbeads 15 for use in the present invention (i.e. for entrapping microbial insect pathogens) it is preferred to use a nucleic acid : protein ratio in the range from about 1:5 to 5:1. If the insect pathogen Bacillus thurin~iensis is to be embedded in the microbeads, it 20 is preferred to use RNA as the nucleic acid and protamine sulfate as the protein, and to use an RNA:
prot~mine sulfate ratio of approximately 1:2.
- In those embodiments of the invention ; wherein it is desired to utilize the microbeads 25 described above, the microbial insect pathogen may be ; embedded (i.e., entrapped) in the microbeads by simply mixing it with an aqueous solution of the desired buffering agent, e.g. phosphate, and then mixing this suspension with an aqueous solution containing the 30 desired nucleic acid. The resulting suspension is then mixed with the aqueous protein solution as described above and the pathogen is spontaneously embedded in the protein-nucleic acid microbeads which form. As an alternative, the microbe may be carried ~ 9785 .~ .
in the protein solution and then mixed with an aqueous solution containing the nucleic acid.
It has been found that subsequent shaking of the vessel in which the solutions have been mixed will 5 cause the microbeads to coalesce and spontaneously reform, usually resulting in additional pathogens being embedded in the microbeads.
While the microbeads produced according to the above-described technique possess a certain degree 10 of stability, (i.e., resistance to bxeakage and coalescence), it may be advantageous to increase their stability to facilitate separation of embedded pathogens from non-embedded pathogens and to further facilitate handling. In one preferred embodiment of 15 the invention, this stabilization is accomplished by chemically crosslinking the microbead protein molecules by treating them with crosslinking agents such as, for example, glutaraldehyde, imidoester agents, dithiobissuccimidyl propionate, etc. If it is desired 20 to use glutaraldehyde, an aqueous solution of 0.25~, or less, (by weight) should be used, since we have found that as the glutaraldehyde concentration is increased, certain pathogens, in particular, Bacillus ~ thuringiensis, will tend to become inactivated.
- 25 It should be noted that the depth of cross-linking can be controlled rather easily by controlling the time, concentration, temperature, and other conditions o~ crosslinking. For example, the depth of crosslinking may be controlled by stopping the cross-30 linking reaction by adding a small molecule which reacts with the crosslinking reagent (e.g. lysine added to glutaraldehyde) or ~y using small crosslinking reagent concentrations.
~uch chemical crosslinking of the microbeads 35 yields several advantages, including: (1) stabili-`~ 9785 zation against the shear forces created by sprayapplication of the insecticide; (2) maintenance, if desired, of fluid centers within the microbeads;
(3) maintenance, if desired, of a pH level inside the 5 microbead which is lower than that of the environment surrounding the microbead (i.e., alkaline digestive juices of the insect gut) so that the interior of the microbead may be kept at a pH value near the optimum pH value for viability, storage, etc. of the microbial 10 pathogen; (4) control of the position in the insect gut where the pathogen is released (i.e. the greater the crosslinking, the further along in the gut release will occur and vice-versa), thereby increasing the infectivity of the pathogen.
We have also found that the microbial insect pathogen may be embedded (i.e. entrapped) in the above-described microbeads much more readily and in much greater numbers if its net surface charge is first modified so as to be made nearly totally - 20 negative or nearly totally positive. This surface charge modification may be accomplished, for example, by the controlled addition of a protein modifying agent such as, for example, succinic anhydride, imidoesters, and similar compounds (see e.g., Gary E.
~5 Means and Robert E. Feeney, Chemical Modifications of Proteins, Holden Day, Inc., 1971). The effectiveness of this charge modification technique will generally be increased by first washing the microbial pathogen composition in separate organic (e.g. 60% ethanol 30 solution, by weight) and inorganic (e.g. lM sodium chloride solution) washes.
Since the charge-modified pathogen apparently competes with the like-charged component of the microbead for positions in the bead, care must 35 be taken to reduce the concentration of such like-ll`~g785 charged component to a level which will permit incorporation of the pathogen into the microbead. For example, if it is desired to entrap Bacillus thur-ingiensis cells, spores, and toxin crystals, all 5 of which have been modified to a strongly negative surface charge, in an RNA-protein microbead as described above, it may be necessary to reduce : slightly the concentration of the RNA solution (RNA
is also negatively charged) prior to mixing with the 10 protein solution.
Likewise, in such a microbead system, if the surface charge of the pathogen has been modified to a strongly positive surface charge, then it may be necessary to reduce slightly the concentration of the 15 protein solution (protein is positively charged) prior to mixing with the RNA solution. This latter system may be more attractive for purposes of the present invention since it does not require reduction of the amount of radiation-absorbing material (i.e. RNA).
Since a primary object of the present invention is the protection of light-sensitive microbial insect pathogens against inactivation by harmful radiation from the sun, it is obviollsly important, in selecting the materials to be used in 25 formulating the microbeads, to select materials which strongly absorb such harmful radiation. While other materials might be selected, the most preferred material is a nucleic acid such as RNA (Ribonucleic Acid), and it is particularly preferred to use a 30 combination of a nucleic acid and a protein. The absorption of microbeads comprised of RNA and protamine, produced according to the above-described technique, is shown in Fig~ 1. Fig. 1 shows the absorption of a 0.1~ solution of microbeads te.g.
``` ~1~9785 - microbeads made by combining 0.1% RNA and 0.1 Protamine) over the solar UV,range.
In many cases the presence of a nucleic acid in the microbead will offer a second advantage. It 5 has been suggested that the damage caused by wave-lengths of sunlight greater than 313 nm is, in the case of many microbes, primarily the result of the reaction of the microbe's nucleic acids with free radicals (it is believed that radiation damage to 10 tyrosine produces H2O2 which, in turn, produces free radicals). The nucleic acid present in the micxobead -' structure will tend to react specifically with the '- free radicals which would otherwise react with the '` microbe's nucleic acids, thus preventing any damage.
Since the pathogenic effect of the microbial agent cannot be realized so long as the agent remains ; embedded within the microbead, care must be taken in selecting the materials to be used in formulating the microbeads to select materials which will permit 20 release of the agent after ingestion of the microbeads by the insect. While other materials might be selected, we have found that microbeads comprised of a protein and a nucleic acid (e.g. RNA) provide quite satisfactory release characteristics.
After ingestion of the microbeads by the - insect, the microbeads will be attacked by proteases and nucleases in the insect digestive tract (i.e., gut), which will lead to release of the microbe.
Thus, it is important to select microbead materials 30 which are not resistant to such type of attack. We have found that if Bacillus thuringiensis (cells, spores and toxin crystals) embedded in microbeads comprised of RNA and protamine (produced according to the above-described technique) are incubated at room 35 temperature in the presence of insect digestive ~:IV9785 juices, release of the B.t. begins within minutes, -with progressive and complete release following within one half hour.
We have also found that if Bacillus 5 thuringiensis embedded in microbeads comprised of RNA
and protamine (produced according to the above-described technique) are incubated at room temperature in the presence of amino acids and/or sugars such as would be found in an insect digestive tract, the 10 germinating spores themselves dissolve the microbeads in approximately two hours. This does not occur in water or buffer alone, so that the microbeads will - remain intact on leaf surfaces.
- It should be noted that, while the only lS microbial insect pathogen discussed in detail above is the bacterial pathogen Bacillus thuringiensis, the present invention is suitable for use with any light sensitive microbial insect pathogen, including those of viral origin. Example 6, below, shows the 20 applicability of the present invention to a bacterial virus. The positive results shown in Example 6 indicate that the present invention should also be suitable for use in protecting insect virus.
Indus-trial Applicability The following Examples illustrate several different embodiments of the present invention. It is intended that all matter in these Examples and in the foregoing description of the preferred embodiments and accompanying drawings be interpreted as merely 30 illustrative and not in a limiting sense.
Example 1 1 x 109 spores of Bacillus thuringiensis, including bacterial cells, spores and asporal ~i~9785 (crystalline) bodies, obtained from a sporulation medium culture, were mixed in lO ml of a .15 N
phosphate buffer at pH 7.5. 1.5 ml of this solution was mixed with 1.5 ml of a buffered 1.34~ aqueous 5 solution (by weight) of yeast RNA (obtained from Sigma as yrade B). Then 0.4 ml of this suspension was mixed with constant stirring in 1.9 ml of a buffered .36% aqueous solution ~by weight) of protamine sulfate (obtained from Sigma as grade B).
RNA-protamine microbeads formed sponta-neously, each entrapping some of the bacterial cells and/or spores and/or asporal bodies. Shaking the mixture resulted in breakage and subsequent spontaneous reformation of additional microbeads. The 15 microbeads were placed in a glass petri dish and exposed to a General Electric G30T8 30 watt germicidal lamp. The petri dishes were placed on a rotary shaker 78 cm below the lamp and shaken a~ 40 rpm.
Viability was determined by plating on brain heart 20 infusion agar obtained from Difco.
When exposed to germicidal ultraviolet radiation tpeak radiation at 254 nm~ sufficient to kill 99.99% of any unprotected B t., the B.t. which was embedded in the microbeads (i.e., the protected 25 bacterial cells and/or spores and/or asporal bodies) nearly all survived. This is shown in Fig. 2. The early die-off shown by the line marked "protected B.t." is thought to be due to the low percentage of B.t. actually embedded in the microbeads. Under 30 microscopic observation the percentage of B.t.
actually embedded was observed to range from 0.5~ to 1.5%.
11;~)9785 Example 2 Microbeads with B.t. embedded therein were - prepared as in Example 1, and then 1 mg/ml dithiobissuccimidyl propionate in DMSO was added to 5 crosslink and stabilize the microbeads. 0.2 ml of this solution were placed in a 0.22 ~ Millipore filter and allowed to dry under vacuum. The filters were ` exposed as in Example 1 without shaking. After shaking, the filters were washed off in dilution 10 buffer and plated as in Example 1. Results of this procedure are shown in Fig. 3.
- Example 3 l x 109 spores of B.t. obtained from culture in sporulation medium were first washed in a 15 60% ethanol solution and then washed in a l M NaCl solution, with the B.t. being separated from these ~- washes by centrifugation. The washed B.t. was then suspended in 20 ~l of a l M sodium carbonate bugger solution at a pH of 8Ø
Next, dry succinic anahydride, a protein modifying agent, was added to the suspension as six separate additions of 2.5 mg/ml each. The additions were made under constant stirring and the mixture was stirred for 10 minutes between each addition. The pH
25 was held at 8.0 + 0.1 by addition of NaOH. When the -- reaction was completed, as indicated by the pH
ceasing to change, the B.t. was separated out (centrifuged) and washed.
This modified B.t. (negati~ely charged) was 30 then incorporated into RNA-protamine microbeads according to the procedures set forth in Example l and the microbeads were crosslinked as in Example 2.
- It was found that this modified B.t.
entered the micxobeads much more readily and in much ', higher numbers than the unmodified B.t. used in Examples 1 and 2. Presumably this was due to the modification of the surface charge on the B.t. from ` positive to negative.
Example 4 A solution of unprotected B.t. (cells, spores, and toxin crystals) and protected B.t.
(i.e., embedded in microbeads as described in Example 3, but without crosslinking), 60% unprotected 10 and 40~ protected (determined microscopically), was subjected to 254 nm radiation as described in Example 1.
Essentially no unprotected spores remained viable after 15 minutes of such irradiation, while 1 x 106 protected spores (i.e., 40% of the total 15 original mixture) remained viable after one hour of such irradiation. Viability was determined as in Example 1. ~he results of this experiment are shown in Fig. 4.
Example 5 A concentration of 1 x 109 spores of - Bacil-lus thuringiensis (including cells, spores and asporal crystals) of B t. was suspended in 10 ml of .15 N phosphate buffer, pH 7.5 (B.t. preparation was obtained and modified as in Example 3). 0.5 grams of 25 RNA (Calbiochem, grade B) was dissolved into this suspension and mixed by vigorous mixing in a Vortex mixing device. The suspension was then added to a buffered 10% solution of protamine sulfate (Calbiochem, grade B, by weight) and vigorously shaken for 5 30 seconds. Glutaraldehyde (25%, from Sigma) was added to the solution to a final concentration of 0.15~ (by volume). After 30 minutes a pellet formed at the bottom of the tube which consisted of large microbeads -" iL1~978S
(100 - 150 ~). The supernate was drawn off and the pellet was resuspended to a final volume of 20 ml by shaking. This solution was placed in a 0.22 ~
Millipore filter and dried overnight under vacuum.
5 The filters were exposed to sunlight (1:00 pm, RH 23%, temperature 89F). The filters were then washed in acetate buffer (.15 N, pH 4.0) to break up the microbeads and release the B.t., which was plated as in Example 1.
The results of this experiment are shown in Fig. 5. Unprotected spores were nearly all killed after 30 minutes.
Example 6 The purpose of this example was to 15 demonstrate protection of a virus according to the present invention. The reactions and responses of an insect virus and a bacterial virus (called bacterial phage) should be similar since both are composed basically of a nucleic acid in a protein coat.
20 Accordingly, we chose to model our system with the bac~erial vir1ls of _ coli, phage T-4.
T-4 bacterial phages were grown in nutrient broth with 0.5% NaCl (P-broth). E. coli BB was inoculated into 100 ml P-broth and allowed to grow 25 overnight. In the morning a 1:100 dilution was made to fresh broth and growth was allowed to proceed for - one hour. 1 x 107 phages were added to this rapidly growing E. coli BB culture and allowed to grow for six hours (37C, rapid shaking). At the end of the 30 period, 5 drops of chloroform were added to kill all bacteria in the culture. This is the phage stock.
Microbeads were prepared by mixing 0.100 grams of protamine sulfate (Calbiochem, grade B) in 10 ml phage stock. This suspension was added to 1~
~1~9~8S
., RNA ~by weight) in P-broth. The microbeads formed spontaneously. The UV exposure was carried out as in Example 1. Timed samples were taken and dilutions were made in P-broth. The viable phages were deter-5 mined by the method described in the following text:
Grace C. Rovozzo and Carroll N. ~urk, A Manual of Basic Virological Techniques, Prentice-Hall Biological Techniques Series, 1973, page 168, using P-broth agar and E. coli BB as the indicator bacteria.
The results of this experiment are shown in Fig. 6. These data show that unprotected virus were all killed in approximately 5 minutes. On the other hand, after an initial drop similar to that seen in the bacterial tests, the virus which were embedded in 15 the micro~eads show strong UV light resistance - (approximately 40~ are protected from inactivation).
It should be understood that the term "nucleic acid" as used throughout this specification and in the claims is intended to include all 20 polynucleotides. ~iXewise, the term "protein" is intended to include all polypeptides.
It is also to be understood that although the present invention has been specifically disclosed by preferred embodiments and optional features, 25 modification and variation of the concepts herein disclosed may be resorted to by those skilled in the art, and such modifications and variations are considered to be within the scope of this invention as defined by the appended claims.
`
MICROBI`AL INSECTICIDES
;- Technical Field ; This invention relates to microbial insecticides. More particularly, the invention relates to a novel microbial insecticide composition 5 and to the production and utilization thereof.
Background Art Microbial insecticides of viral or bacterial origin offer signi-ficant advantages over conventional chemical insecticides. Microbial insect pathogens are lO generally nontoxic and harmless to other forms of life.
In addition, microbial insecticides demonstrate a relatively high degree of specificity, and hence do not endanger beneficial insects. Moreover, a suscep-tible insect host is quite slow to develop resistance 15 to microbial pathogens. Microbial insecticides may be used in relati~ely low dosages, may be effectively applied as dusts or sprays, and may be used in combination with chemical insecticides.
For example, Bacillus thuringiensis (B.t.), 20 a spore-forming bacterium, is well-known as a micro-bial insect pathogen useful against numerous leaf-chewing insects in their larval stages, including, for example, alfalfa caterpillars, tomato hornworms, ., ~
~1~)9'785 tobacco hornworms, cabbage loopers, cabbage web worms, army worms, gypsy moths, walnut caterpillars, diamond-back moths, cosmopolitan green beetles, European corn borers, and other members of the order Lepidoptera.
Unfortunately, the effectiveness and usefulness in the field of many microbial insect pathogens as insecticides is severly limited by their extreme sensitivity to sunlight. It is known that nonionizing radiation having a high photon energy 10 (e.g. ultraviolet rays o~ sunlight) exerts an inactivating effect on various microbial insect pathogens. See, e.g., "Photoprotection Against Inactivation of Bacillus thuringiensis Spores by Ultra~iolet Rays," Aloysius Krieg, Journal of 15 Invertebrate Patholo~y, Vol. 25, pp. 267-268 (1975).
For example, it is known that ultraviolet (W) rays with a wavelength of 253.7 nm induce a marked, extra-- ordinary inactivation of Bacillus thurin~iensis (B.t.) spores, so that they are unable to germinate and grow 20 out. A dosage of 18 m W sec/cm2 of such UV radiation will inactivate 99.9% of the B.t. spores. ~e have determined that the half life of B.t. subjected to W
radiation of sunlight in the field is approximately six minutes. ~i~ewise, it has been determined by 25 others that the half lives of certain occluded viruses subjected to W radiation of sunlight in the field is - one and one-half hours. Thus, the effectiveness of a typical spray application of such microbial insecticides is rapidly lost in the field.
Since nucleic acids show a maximum of - extinction near a wavelength of 260 nm, it is believed that the UV induced death of B.t. at 253.7 Nm, and of certain occluded viruses at comparable wavelengths, may be caused by a photoreaction of the genetic 35 material, especially DNA. Thus, it has been suggested, li`()9785 ibid., at p. 267, that B.t. spores could be protected from inactivation by UV
radiation by physically mixing the B.t. spores with DNA, or a comparable nucleic acid which would absorb the UV rays. Such a comparable nucleic acid would be RNA, Ribonucleic Acidl which has a maximum of extinction near 260 nm.
However, this technique proved to be ineffective.
Disclosure of Invention In one aspect, the present invention provides an insecticidal composi-tion comprising a microbial agent in combination with at least one of its bio-logically active chemical products embedded in a coacervate microbead having an effective diameter within the range of from about lO to about 150 microns which is comprised of a nucleic acid and a proteinaceous material admixed in a nucleicacid to proteinaceous material ratio in the range of from about 1:5 to about 5:1.
In another aspect the invention provides a method of embedding micro-organisms and their biologically active chemical products in microbeads, comprising:
(a) mixing a buffered aqueous solution containing microorganisms and - their biologically active chemical products with an aqueous solution containing - a nucleic acid; and (b) mixing the said mixture with an aqueous solution containing a proteinaceous material, thereby spontaneously forming microbeads having the microorganisms and chemical products embedded therein.
In the present invention, materials capable of absorbing radiation which would otherwise inactivate the microbial insect pathogen of concern are incorporated in a protective coating which surrounds the pathogen in the form of a microbead. These materials serve to intercept and block the harmful radia-tion before it reaches the light-sensitive material of the insect pathogen. In a typical embodiment of the invention the material selected to intercept the harmful radiation comprises a nucleic acid; and in a preferred embodiment the iL1~9785 material comprises R~A. Various other materials and combinations of materials may be used in combination witll tlle nucleic acid depending on the specific in-sect pathogen to be protected and the microbead coating system to be used;
including, but not limited to the following materials: protamine, cytochrome c, soy protein, hemoglobin, gelatin, etc. In general, any protein can be used if the conditions are adjusted as to facilitate formation of the microbeads. Such conditions may include charge modification techniques, adjustments in pH, component concentrations, etc.
~he microbeads in which the microbial insect pathogen are embedded may be advantageously produced using any known technique for forming what have been called coacervate droplets or microbeads. One such - 3a -~1~978S
technique was developed in conjunction with the study of the origin of life on earth, and has been used to construct precellular models. See, for example, Evreinova, et al., Journal of Colloid and Interface 5 Science, Vol. 36, No. 1 (1971).
Brief Description of Drawings Fig. 1 is a graph showing the optical density (i.e., absorption) over the solar UV range of typical microbeads suitable for use in the present 10 invention.
Figs. 2-6 are graphs showing the comparative experimental data from Examples 1, 2, 4, 5, and 6, below, respectively. In Figs. 2-4 the number of viable spores, extrapolated to 1 ml of original 15 sample, is shown as a function of the length of time of exposure to the W radiation. In Figs. 5 and 6 the percentage of B.t. remaining as survivors is shown as a function of the exposure time.
Best Mode for Carrying Out the Invention In one preferred em~odiment of the invention the microbeads axe produced using the above-mentioned technique as follows. An aqueous solution containing a nucleic acid such as, for example, RNA, and a buffering agent such as, for example, phosphate, 25 designed to maintain a pH at the position which optimizes the charge on the RNA, the protein and the microbe (where the microbe is sensitive to pH, this should be taken into account as well), is mixed with an aqueous solution containing an appropriate protein 30 material, such as, for example, protamine, gelatin, soy isolate, hemoglobin, etc. or synthetic amino acid polymers. Protein-nucleic acid microbeads which are 11~9785 essentially solid and roughly spherical form spontaneously upon the mixing of these two solutions.
It is important to note that while all of the above-named protein materials, and others, can be 5 used satisfactorily in forming the microbeads, care must be taken to maintain the pH of the mixture of solutions on the acid side of the isoelectric point of the particular protein being used since this is required for formation of the microbeads. If a 10 protein is used that is insoluble at a given pH, the p~ will have to be put in a range tn which the protein is soluble, or other steps will have to be taken to make the protein soluble. These steps could include partial degradation, charge modification or adding 15 other components in the buffers (e.g. detergents, alcohols, surfactants, etc.). If foaming of one or more components is a problem, simethicone type agents (U.S. patent No. 2,441,098) can be included. When RNA
is being used as the nucleic acid, the pH of the mix-20 ture of solutions must be maintained at or above about4.3 to prevent the RNA from precipitating out of the microbead, with the protein necessarily leaving the microbead and going back into solution.
As can be seen, in the above-described method 25 for forming microbeads (i.e., coacervate droplets) the material to be utilized to intercept and absorb the harmful radiation, i.e., the nucleic acid, is incor-porated directly in the microbead structure, thereby producing a highly protective coating.
The bimolecular structure of the microbeads creates a thermodynamically stable cooperation between the components, so that even without subsequent chemical crosslinking, as described below, the components will not individually diffuse ou~ of the 35 microbeads. The bimolecular structure also causes the 11~9785 microbeads to be highly charged. These charges should aid the microbeads in sticking to plant surfaces.
These charges can be controlled by selecting the appropriate protein to be used in forming the 5 microbead.
The size of the microbeads can be controlled by controlling the concentration of the nucleic acid and the protein in the formation vessel. For example, 100~ beads can be made by mixing 5~ RNA with 10%
10 protamine sulfate. Beads will form and settle to the bottom of the vessel. Most of these will be in the 100~ range. While a relatively wide range of nucleic acid and protein concentrations can be used to make these microbeads generally, in preparing microbeads 15 for use in the present invention (i.e. for entrapping microbial insect pathogens) it is preferred to use a nucleic acid : protein ratio in the range from about 1:5 to 5:1. If the insect pathogen Bacillus thurin~iensis is to be embedded in the microbeads, it 20 is preferred to use RNA as the nucleic acid and protamine sulfate as the protein, and to use an RNA:
prot~mine sulfate ratio of approximately 1:2.
- In those embodiments of the invention ; wherein it is desired to utilize the microbeads 25 described above, the microbial insect pathogen may be ; embedded (i.e., entrapped) in the microbeads by simply mixing it with an aqueous solution of the desired buffering agent, e.g. phosphate, and then mixing this suspension with an aqueous solution containing the 30 desired nucleic acid. The resulting suspension is then mixed with the aqueous protein solution as described above and the pathogen is spontaneously embedded in the protein-nucleic acid microbeads which form. As an alternative, the microbe may be carried ~ 9785 .~ .
in the protein solution and then mixed with an aqueous solution containing the nucleic acid.
It has been found that subsequent shaking of the vessel in which the solutions have been mixed will 5 cause the microbeads to coalesce and spontaneously reform, usually resulting in additional pathogens being embedded in the microbeads.
While the microbeads produced according to the above-described technique possess a certain degree 10 of stability, (i.e., resistance to bxeakage and coalescence), it may be advantageous to increase their stability to facilitate separation of embedded pathogens from non-embedded pathogens and to further facilitate handling. In one preferred embodiment of 15 the invention, this stabilization is accomplished by chemically crosslinking the microbead protein molecules by treating them with crosslinking agents such as, for example, glutaraldehyde, imidoester agents, dithiobissuccimidyl propionate, etc. If it is desired 20 to use glutaraldehyde, an aqueous solution of 0.25~, or less, (by weight) should be used, since we have found that as the glutaraldehyde concentration is increased, certain pathogens, in particular, Bacillus ~ thuringiensis, will tend to become inactivated.
- 25 It should be noted that the depth of cross-linking can be controlled rather easily by controlling the time, concentration, temperature, and other conditions o~ crosslinking. For example, the depth of crosslinking may be controlled by stopping the cross-30 linking reaction by adding a small molecule which reacts with the crosslinking reagent (e.g. lysine added to glutaraldehyde) or ~y using small crosslinking reagent concentrations.
~uch chemical crosslinking of the microbeads 35 yields several advantages, including: (1) stabili-`~ 9785 zation against the shear forces created by sprayapplication of the insecticide; (2) maintenance, if desired, of fluid centers within the microbeads;
(3) maintenance, if desired, of a pH level inside the 5 microbead which is lower than that of the environment surrounding the microbead (i.e., alkaline digestive juices of the insect gut) so that the interior of the microbead may be kept at a pH value near the optimum pH value for viability, storage, etc. of the microbial 10 pathogen; (4) control of the position in the insect gut where the pathogen is released (i.e. the greater the crosslinking, the further along in the gut release will occur and vice-versa), thereby increasing the infectivity of the pathogen.
We have also found that the microbial insect pathogen may be embedded (i.e. entrapped) in the above-described microbeads much more readily and in much greater numbers if its net surface charge is first modified so as to be made nearly totally - 20 negative or nearly totally positive. This surface charge modification may be accomplished, for example, by the controlled addition of a protein modifying agent such as, for example, succinic anhydride, imidoesters, and similar compounds (see e.g., Gary E.
~5 Means and Robert E. Feeney, Chemical Modifications of Proteins, Holden Day, Inc., 1971). The effectiveness of this charge modification technique will generally be increased by first washing the microbial pathogen composition in separate organic (e.g. 60% ethanol 30 solution, by weight) and inorganic (e.g. lM sodium chloride solution) washes.
Since the charge-modified pathogen apparently competes with the like-charged component of the microbead for positions in the bead, care must 35 be taken to reduce the concentration of such like-ll`~g785 charged component to a level which will permit incorporation of the pathogen into the microbead. For example, if it is desired to entrap Bacillus thur-ingiensis cells, spores, and toxin crystals, all 5 of which have been modified to a strongly negative surface charge, in an RNA-protein microbead as described above, it may be necessary to reduce : slightly the concentration of the RNA solution (RNA
is also negatively charged) prior to mixing with the 10 protein solution.
Likewise, in such a microbead system, if the surface charge of the pathogen has been modified to a strongly positive surface charge, then it may be necessary to reduce slightly the concentration of the 15 protein solution (protein is positively charged) prior to mixing with the RNA solution. This latter system may be more attractive for purposes of the present invention since it does not require reduction of the amount of radiation-absorbing material (i.e. RNA).
Since a primary object of the present invention is the protection of light-sensitive microbial insect pathogens against inactivation by harmful radiation from the sun, it is obviollsly important, in selecting the materials to be used in 25 formulating the microbeads, to select materials which strongly absorb such harmful radiation. While other materials might be selected, the most preferred material is a nucleic acid such as RNA (Ribonucleic Acid), and it is particularly preferred to use a 30 combination of a nucleic acid and a protein. The absorption of microbeads comprised of RNA and protamine, produced according to the above-described technique, is shown in Fig~ 1. Fig. 1 shows the absorption of a 0.1~ solution of microbeads te.g.
``` ~1~9785 - microbeads made by combining 0.1% RNA and 0.1 Protamine) over the solar UV,range.
In many cases the presence of a nucleic acid in the microbead will offer a second advantage. It 5 has been suggested that the damage caused by wave-lengths of sunlight greater than 313 nm is, in the case of many microbes, primarily the result of the reaction of the microbe's nucleic acids with free radicals (it is believed that radiation damage to 10 tyrosine produces H2O2 which, in turn, produces free radicals). The nucleic acid present in the micxobead -' structure will tend to react specifically with the '- free radicals which would otherwise react with the '` microbe's nucleic acids, thus preventing any damage.
Since the pathogenic effect of the microbial agent cannot be realized so long as the agent remains ; embedded within the microbead, care must be taken in selecting the materials to be used in formulating the microbeads to select materials which will permit 20 release of the agent after ingestion of the microbeads by the insect. While other materials might be selected, we have found that microbeads comprised of a protein and a nucleic acid (e.g. RNA) provide quite satisfactory release characteristics.
After ingestion of the microbeads by the - insect, the microbeads will be attacked by proteases and nucleases in the insect digestive tract (i.e., gut), which will lead to release of the microbe.
Thus, it is important to select microbead materials 30 which are not resistant to such type of attack. We have found that if Bacillus thuringiensis (cells, spores and toxin crystals) embedded in microbeads comprised of RNA and protamine (produced according to the above-described technique) are incubated at room 35 temperature in the presence of insect digestive ~:IV9785 juices, release of the B.t. begins within minutes, -with progressive and complete release following within one half hour.
We have also found that if Bacillus 5 thuringiensis embedded in microbeads comprised of RNA
and protamine (produced according to the above-described technique) are incubated at room temperature in the presence of amino acids and/or sugars such as would be found in an insect digestive tract, the 10 germinating spores themselves dissolve the microbeads in approximately two hours. This does not occur in water or buffer alone, so that the microbeads will - remain intact on leaf surfaces.
- It should be noted that, while the only lS microbial insect pathogen discussed in detail above is the bacterial pathogen Bacillus thuringiensis, the present invention is suitable for use with any light sensitive microbial insect pathogen, including those of viral origin. Example 6, below, shows the 20 applicability of the present invention to a bacterial virus. The positive results shown in Example 6 indicate that the present invention should also be suitable for use in protecting insect virus.
Indus-trial Applicability The following Examples illustrate several different embodiments of the present invention. It is intended that all matter in these Examples and in the foregoing description of the preferred embodiments and accompanying drawings be interpreted as merely 30 illustrative and not in a limiting sense.
Example 1 1 x 109 spores of Bacillus thuringiensis, including bacterial cells, spores and asporal ~i~9785 (crystalline) bodies, obtained from a sporulation medium culture, were mixed in lO ml of a .15 N
phosphate buffer at pH 7.5. 1.5 ml of this solution was mixed with 1.5 ml of a buffered 1.34~ aqueous 5 solution (by weight) of yeast RNA (obtained from Sigma as yrade B). Then 0.4 ml of this suspension was mixed with constant stirring in 1.9 ml of a buffered .36% aqueous solution ~by weight) of protamine sulfate (obtained from Sigma as grade B).
RNA-protamine microbeads formed sponta-neously, each entrapping some of the bacterial cells and/or spores and/or asporal bodies. Shaking the mixture resulted in breakage and subsequent spontaneous reformation of additional microbeads. The 15 microbeads were placed in a glass petri dish and exposed to a General Electric G30T8 30 watt germicidal lamp. The petri dishes were placed on a rotary shaker 78 cm below the lamp and shaken a~ 40 rpm.
Viability was determined by plating on brain heart 20 infusion agar obtained from Difco.
When exposed to germicidal ultraviolet radiation tpeak radiation at 254 nm~ sufficient to kill 99.99% of any unprotected B t., the B.t. which was embedded in the microbeads (i.e., the protected 25 bacterial cells and/or spores and/or asporal bodies) nearly all survived. This is shown in Fig. 2. The early die-off shown by the line marked "protected B.t." is thought to be due to the low percentage of B.t. actually embedded in the microbeads. Under 30 microscopic observation the percentage of B.t.
actually embedded was observed to range from 0.5~ to 1.5%.
11;~)9785 Example 2 Microbeads with B.t. embedded therein were - prepared as in Example 1, and then 1 mg/ml dithiobissuccimidyl propionate in DMSO was added to 5 crosslink and stabilize the microbeads. 0.2 ml of this solution were placed in a 0.22 ~ Millipore filter and allowed to dry under vacuum. The filters were ` exposed as in Example 1 without shaking. After shaking, the filters were washed off in dilution 10 buffer and plated as in Example 1. Results of this procedure are shown in Fig. 3.
- Example 3 l x 109 spores of B.t. obtained from culture in sporulation medium were first washed in a 15 60% ethanol solution and then washed in a l M NaCl solution, with the B.t. being separated from these ~- washes by centrifugation. The washed B.t. was then suspended in 20 ~l of a l M sodium carbonate bugger solution at a pH of 8Ø
Next, dry succinic anahydride, a protein modifying agent, was added to the suspension as six separate additions of 2.5 mg/ml each. The additions were made under constant stirring and the mixture was stirred for 10 minutes between each addition. The pH
25 was held at 8.0 + 0.1 by addition of NaOH. When the -- reaction was completed, as indicated by the pH
ceasing to change, the B.t. was separated out (centrifuged) and washed.
This modified B.t. (negati~ely charged) was 30 then incorporated into RNA-protamine microbeads according to the procedures set forth in Example l and the microbeads were crosslinked as in Example 2.
- It was found that this modified B.t.
entered the micxobeads much more readily and in much ', higher numbers than the unmodified B.t. used in Examples 1 and 2. Presumably this was due to the modification of the surface charge on the B.t. from ` positive to negative.
Example 4 A solution of unprotected B.t. (cells, spores, and toxin crystals) and protected B.t.
(i.e., embedded in microbeads as described in Example 3, but without crosslinking), 60% unprotected 10 and 40~ protected (determined microscopically), was subjected to 254 nm radiation as described in Example 1.
Essentially no unprotected spores remained viable after 15 minutes of such irradiation, while 1 x 106 protected spores (i.e., 40% of the total 15 original mixture) remained viable after one hour of such irradiation. Viability was determined as in Example 1. ~he results of this experiment are shown in Fig. 4.
Example 5 A concentration of 1 x 109 spores of - Bacil-lus thuringiensis (including cells, spores and asporal crystals) of B t. was suspended in 10 ml of .15 N phosphate buffer, pH 7.5 (B.t. preparation was obtained and modified as in Example 3). 0.5 grams of 25 RNA (Calbiochem, grade B) was dissolved into this suspension and mixed by vigorous mixing in a Vortex mixing device. The suspension was then added to a buffered 10% solution of protamine sulfate (Calbiochem, grade B, by weight) and vigorously shaken for 5 30 seconds. Glutaraldehyde (25%, from Sigma) was added to the solution to a final concentration of 0.15~ (by volume). After 30 minutes a pellet formed at the bottom of the tube which consisted of large microbeads -" iL1~978S
(100 - 150 ~). The supernate was drawn off and the pellet was resuspended to a final volume of 20 ml by shaking. This solution was placed in a 0.22 ~
Millipore filter and dried overnight under vacuum.
5 The filters were exposed to sunlight (1:00 pm, RH 23%, temperature 89F). The filters were then washed in acetate buffer (.15 N, pH 4.0) to break up the microbeads and release the B.t., which was plated as in Example 1.
The results of this experiment are shown in Fig. 5. Unprotected spores were nearly all killed after 30 minutes.
Example 6 The purpose of this example was to 15 demonstrate protection of a virus according to the present invention. The reactions and responses of an insect virus and a bacterial virus (called bacterial phage) should be similar since both are composed basically of a nucleic acid in a protein coat.
20 Accordingly, we chose to model our system with the bac~erial vir1ls of _ coli, phage T-4.
T-4 bacterial phages were grown in nutrient broth with 0.5% NaCl (P-broth). E. coli BB was inoculated into 100 ml P-broth and allowed to grow 25 overnight. In the morning a 1:100 dilution was made to fresh broth and growth was allowed to proceed for - one hour. 1 x 107 phages were added to this rapidly growing E. coli BB culture and allowed to grow for six hours (37C, rapid shaking). At the end of the 30 period, 5 drops of chloroform were added to kill all bacteria in the culture. This is the phage stock.
Microbeads were prepared by mixing 0.100 grams of protamine sulfate (Calbiochem, grade B) in 10 ml phage stock. This suspension was added to 1~
~1~9~8S
., RNA ~by weight) in P-broth. The microbeads formed spontaneously. The UV exposure was carried out as in Example 1. Timed samples were taken and dilutions were made in P-broth. The viable phages were deter-5 mined by the method described in the following text:
Grace C. Rovozzo and Carroll N. ~urk, A Manual of Basic Virological Techniques, Prentice-Hall Biological Techniques Series, 1973, page 168, using P-broth agar and E. coli BB as the indicator bacteria.
The results of this experiment are shown in Fig. 6. These data show that unprotected virus were all killed in approximately 5 minutes. On the other hand, after an initial drop similar to that seen in the bacterial tests, the virus which were embedded in 15 the micro~eads show strong UV light resistance - (approximately 40~ are protected from inactivation).
It should be understood that the term "nucleic acid" as used throughout this specification and in the claims is intended to include all 20 polynucleotides. ~iXewise, the term "protein" is intended to include all polypeptides.
It is also to be understood that although the present invention has been specifically disclosed by preferred embodiments and optional features, 25 modification and variation of the concepts herein disclosed may be resorted to by those skilled in the art, and such modifications and variations are considered to be within the scope of this invention as defined by the appended claims.
Claims (12)
PROPERTY OR PRIVILEGE IS CLAIMED ARE DEFINED AS FOLLOWS:
1. An insecticidal composition comprising a microbial agent in combina-tion with at least one of its biologically active chemical products embedded in a coacervate microbead having an effective diameter within the range of from about 10 to about 150 microns which is comprised of a nucleic acid and a pro-teinaceous material admixed in a nucleic acid to proteinaceous material ratio in the range of from about 1:5 to about 5:1.
2. The insecticidal composition of claim 1, wherein the microbead is further stabilized by chemical crosslinking.
3. The insecticidal composition of claim 1, wherein the nucleic acid comprises ribonucleic acid.
4. The insecticidal composition of claim 1, wherein the proteinaceous material comprises hemoglobin, protamine, or a synthetic amino acid polymer.
5. The insecticidal composition of claim 1, wherein the effective dia-meter of the microbead is within the range from about 40 to about 100 microns.
6. An insecticidal composition comprising a microbial agent in combination with at least one of its biologically active chemical products, said microbial agent and chemical products being susceptible to environmentally-induced inacti-vation and being embedded in a coacervate microbead having an effective diameter within the range of from about 10 to about 150 microns which is comprised of a nucleic acid and a proteinaceous material admixed in a nucleic acid to protein-aceous material ratio in the range of from about 1:5 to about 5:1, whereby the microbead structure effectively shields said agent and chemical products from environmentally-induced inactivation.
7. An insecticidal composition as claimed in claim 6, wherein the micro-bial agent and chemical products are susceptible to sunlight-induced inactiva-tion and wherein the microbead structure effectively shields them from such inactivation.
8. The insecticidal composition of claim 1 or 6 wherein the micro-organisms and chemical products possess an essentially uniform surface charge when they are embedded in the microbead.
9. A method for controlling insect pests in insect-infested areas, which comprises applying an effective amount of the insecticide composition of claim 1 or 6 to said insect-infested areas.
10. A method of embedding microorganisms and their biologically active chemical products in microbeads, comprising:
(a) mixing a buffered aqueous solution containing microorganisms and their biologically active chemical products with an aqueous solution containing a nucleic acid; and (b) mixing the said mixture with an aqueous solution containing a proteinaceous material, thereby spontaneously forming microbeads having the microorganisms and chemical products embedded therein.
(a) mixing a buffered aqueous solution containing microorganisms and their biologically active chemical products with an aqueous solution containing a nucleic acid; and (b) mixing the said mixture with an aqueous solution containing a proteinaceous material, thereby spontaneously forming microbeads having the microorganisms and chemical products embedded therein.
11. A method as in claim 10, wherein the microbeads are subsequently sta-bilized by treatment with chemical crosslinking agents.
12. A method as in claim 10 wherein prior to the two mixing steps the microorganisms and chemical products are cleaned by washing and the surface charge of the microorganisms and chemical products is modified by the addition of a protein-modifying agent adapted to provide a more uniform surface charge.
Applications Claiming Priority (2)
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US83581777A | 1977-09-22 | 1977-09-22 | |
US835,817 | 1992-02-18 |
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CA1109785A true CA1109785A (en) | 1981-09-29 |
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CA311,958A Expired CA1109785A (en) | 1977-09-22 | 1978-09-22 | Microbial insecticides |
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BE (1) | BE870651A (en) |
CA (1) | CA1109785A (en) |
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GB (1) | GB2020976B (en) |
IE (1) | IE47370B1 (en) |
IT (1) | IT1099109B (en) |
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FR2545326B1 (en) * | 1983-05-02 | 1988-05-20 | Solvay | COMPOSITIONS CONTAINING BIOSYNTHETIC PESTICIDE PRODUCTS AND AT LEAST ONE PHOSPHATE AND METHODS FOR THEIR MANUFACTURE AND USE |
US4615883A (en) * | 1985-10-23 | 1986-10-07 | Plant Genetics, Inc. | Hydrogel encapsulated nematodes |
AP274A (en) * | 1986-06-03 | 1993-03-04 | Dow Chemical Co | Pesticidal compositions and process for preparation thereof. |
DE3870061D1 (en) * | 1987-06-19 | 1992-05-21 | Univ Temple | MICROCAPSULES WITH STEERED RELEASE AND METHOD FOR THE PRODUCTION THEREOF. |
AU631359B2 (en) * | 1988-02-12 | 1992-11-26 | President And Fellows Of Harvard College | Encapsulated bacterium |
JPH0656615A (en) * | 1992-07-31 | 1994-03-01 | Central Glass Co Ltd | Agricultural chemical of microorganism |
FR2848854B1 (en) * | 2002-12-24 | 2005-03-18 | Coletica | PARTICLES COMPRISING A BIOPOLYMER DEGRADABLE UNDER THE EFFECT OF AN ELECTROMAGNETIC WAVE AS EMITTED BY SOLAR RADIATION |
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US3337395A (en) * | 1963-12-27 | 1967-08-22 | Robert Z Page | Termite control by induced epizootics of entomophagous microorganisms |
US3791983A (en) * | 1967-08-11 | 1974-02-12 | Ncr | Sprayable and aerosolizable webforming compositions |
US3541203A (en) * | 1969-05-08 | 1970-11-17 | Ncr Co | Protected virus composition for insect control |
US4056610A (en) * | 1975-04-09 | 1977-11-01 | Minnesota Mining And Manufacturing Company | Microcapsule insecticide composition |
CH619352A5 (en) * | 1975-07-18 | 1980-09-30 | Sandoz Ag | Insecticidal compositions |
-
1978
- 1978-09-12 WO PCT/US1978/000080 patent/WO1979000155A1/en unknown
- 1978-09-12 GB GB7914833A patent/GB2020976B/en not_active Expired
- 1978-09-19 IT IT27813/78A patent/IT1099109B/en active
- 1978-09-19 IE IE1892/78A patent/IE47370B1/en unknown
- 1978-09-20 FR FR7826987A patent/FR2403744A1/en active Pending
- 1978-09-20 NL NL7809563A patent/NL7809563A/en not_active Application Discontinuation
- 1978-09-21 BE BE190618A patent/BE870651A/en not_active IP Right Cessation
- 1978-09-22 CA CA311,958A patent/CA1109785A/en not_active Expired
- 1978-09-22 JP JP11737178A patent/JPS5455722A/en active Pending
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IE47370B1 (en) | 1984-03-07 |
NL7809563A (en) | 1979-03-26 |
FR2403744A1 (en) | 1979-04-20 |
GB2020976B (en) | 1982-08-18 |
IE781892L (en) | 1979-03-22 |
IT7827813A0 (en) | 1978-09-19 |
WO1979000155A1 (en) | 1979-04-05 |
IT1099109B (en) | 1985-09-18 |
BE870651A (en) | 1979-03-21 |
JPS5455722A (en) | 1979-05-04 |
GB2020976A (en) | 1979-11-28 |
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