CA1085822A

CA1085822A - Cephalosporin compounds

Info

Publication number: CA1085822A
Application number: CA240,739A
Authority: CA
Inventors: David A. Berges
Original assignee: Smith Kline and French Canada Ltd
Current assignee: GlaxoSmithKline Inc
Priority date: 1974-12-27
Filing date: 1975-11-28
Publication date: 1980-09-16
Also published as: CH622262A5; BE837071A; DE2558024A1; GB1539518A; FR2303807B1; ES456340A1; IT1051939B; US4142046A; JPS5188989A; ZA756192B; FR2313068B1; NL7513872A; ES442100A1; IE43127B1; FR2313068A1; US3989694A; GB1539517A; AU8789275A; FR2303807A1; IE43127L

Abstract

ABSTRACT
The compounds of this invention are cephalosporins having various acyl substituents at the 7 position and a substituted triazolyl thiomethyl group at the 3-position of the cephem nucleus and intermediates for the preparation thereof.
The 7-acylated compounds have antibacterial activity.

Description

This invention relates to a new series of cephalosporin compounds which have antibacterial activity when administered orally or parenterally and to inter-mediates for the preparation thereof. In particular, the biologically active cephalosporin compounds of this invention have a substituted triazolyl thiomethyl group at the 3-position of the cephem nucleus.
The compounds of this invention are represented by the following structural formula:
Formula I
in which, R1 is phenyl or phenyl substituted with hydroxy, X is amino or hydroxy; and Het is 1,2,4-triazolyl or 1,2,3-triazolyl of which is substituted with -(CHR2)nCOR3, where R2 is hydrogen, n is zero to six and R3 is hydroxy, lower alkoxy, amino, lower alkylamino or di(lower)alkylamino, or a non-toxic pharmaceutically acceptable salt thereof.
As used herein, the terms "lower alkyl" and "lower alkoxy" refer to groups having from one to four carbon atoms; "halo" refers to fluoro, chloro and bromo.

- 2 -Ad~antageouQ compounds of th~s inve~tio~ ~re repre~ented by Formula I wh~re Rl is phenyl or hydroxy~
phenyl; X is amino or hydroxy; Het is 1,293~triazol5~yl is or 1,2,4~triazol~3~yl ea~h of which is substituted wi~h lHR~ ,COR3 J where ~2 ~Ls hydrogen9 n is zero to four and R3 i~ hydroxy, l.ower alkoagy, amlno, lower alkyl-~ino or di(lower)alkylam~oO `:
~st ad~rantageou~ are the compoulld3 repre~nted : :
by FG~mula I where Rl i~ phenyl or 4hydroxyphenyl; X
is ~un~ao or hydro~ey, Het is 19~,3~tria~ol~5~yl or 1,2,4-trlazol-3-yl each o~ whLch is sub~tituted with - ~CHR~)nCoE~3, where R2 1~ hydrogen, n 19 zero ~o four ~nd R~ is hydro~y, lower alkoxy or aminoO
2S Par~icularly pre~rred are the compou~ds 7~D~ ~
m~ndelamido-3-(5-carboxymethyl-1,294 triazol~3~ylthlo~ :
methyl~-3~cephem 4~carbo~gylic acid, 7~(D~a~am~nophenyl~ -a~e ~mido) D3S- (5 ~carboxymethyll 9 2 9 4~triazol~-3~yl~hio~
methyl)~3~cephem~4Dcarboxylic acid9 7~ Damino~4 hydroxyph~nylacetamido ~ ~ 3~ (5~ carboxym~hyl~ 1, 2, 4~
tri~zol 3~ylthiomethyl)w3~cephem~4~carbo~ c acld and 7 ~ (D~aDamino~4~hydroxyphenylacetamido~ ~ 3~ ~4~carbamoyl~

~851!~Z2 1,2,3-triazol-5-ylthiomethyl~3-cephem-4-carboxylic aeld.
Cephalosporin derivatives having a substituted ~-aminophenylaeetamido, substituted mandelamido or ~-amino- or ~-hydroxythienylacetamido group at the 7-position are well documented in the prior art. Sub-stitution by a substituted S-heterocycliethiomethyl group at the 3-position of the eephem nueleus is also known and is diselosed in Netherlands Patent 6916151 where Het is, among others, triazolyl substituted with, inter alia, carboxy, earbalkoxy, alkoxyalkylaminoearbonyl and dialkylaminoalkylaminoearbonyl. These eephalo-sporins, however, have a similarly substituted hetero-eyelic acetamido group at the 7-position. No compounds containing both a 7-substituted ~-aminophenylacetamido, 7-substituted mandelamido or 7-(~-amino- or ~-hydroxy)-thienylacetamido group and the 3-substituted triazolyl-thiomethyl moiety diselosed herein are believed to be known ~Q the art.
The compounds of Formula I are prepared by acylation of an appropriate 7-amino-3-substituted triazolylthiomethyl cephalosporin nueleus of Formula II:

2 ~ S ~

0~~ ~CH2SHet ,, COOR
Formula II
in whieh:

Het is 1,2,4-triazolyl or 1,2,3-triazolyl each of which is substituted with -(CHR2)nCoR3, - . , ~3S8Z;~

where R i5 hydrogen, n is zero to 5iX and R3 is hydroxy, lower alkoxy, amino, lower alkyl- r amino or di~lower~alkylamino; and R~ is hydrogen or a protecting ester group, with an appropriately substituted phenyl or thienyl acetic acid followed by removal of the protective groups. The carboxylic acid group is activated by any of the standard methods such as conversion to the mixed anhydride, acid chloride or activated ester. In addition, a reagent such as dicyclohexylcarbodiimide or carbonyldiimidazole can be used provided that the carboxyl group on the cephem nucleus is protected with an easily removable protecting group such as a benzhydryl, t-butyl, trichloroethyl, benzyl, benzyloxymethyl, p-nitrophenyl, _-methoxyphenyl, p-methoxybenzyl or p~nitrobenzyl ester. When X is amino, the ~-amino group of the phenyl or thienyl acetic acid ;~
moiety is, preferably, protected prior to acylation with an easily removable protective group known in the art such as t-butoxycarbonyl, trichloroethoxycarbonyl, benzyloxy-carbonyl, the methyl acetoacetate adduct or similar groups commonly used in the synthesis of peptides. The compounds represented by Formula II above are also considered as objects of this invention.
Alternatively, the compounds of Formula I are prepared by acylating 7-aminocephalosporanic acid with an appropriately substituted and protected, as described above, phenyl or thienyl acetic acid and then displacing the 3-acetoxy group with the desired substituted triazole thiol with subsequent removal of the protectlve group(s).
The protective groups can be removed according to z~

methods well known to the art, such as ~ith trifluoro-acetic acid when t-~utyl or t-butoxycarbonyl protective groups are used. The resulting salt is converted to the zwitterionic product or to the free acid by means of a basic ion exchange resin such as polystreneamine ion exchange resin (Amberlite IR-45 ~ or else by basification of an aqueous solution of the salt. ;
The substituted phenyl or thienyl acetic acid starting materials are known or are prepared by known methods, for example as described in Belgian Patent 774029 ln the case of substituted phenyl glycines and in U.SO Patents 3,422,099 and 3,352,858 for substituted thienyl acetic acids. Examples of substituted mandelic acids are found in U.S. Patent 3,641,021.
The 7-amino-3-substituted triazolylthiomethyl cephalosporin starting materials of Formula II are prepared from reaction of 7-aminocephalosporanic acid and a substituted triazole thiol.
The substituted 1,2,3-triazole thiols not known ~-to the art are prepared by rearrangement of a corres-pondingly substituted amino thiadiazole according to the procedure of Goerdeler and Gnad [Chem. Ber. 99:1618 (1966)], by conversion of a suitably substituted hydroxy ~ -1,2,3-triazole to the corresponding thiol by the method of Hoover and Day [J. Amer. Chem. Soc. 78:5832 (1956)]
or by reaction of an acetylene carboxylic acid with a substituted azide and subsequent decarboxylation and thiation.
The compounds of this invention are capable of forming salts with, for example, the alkali metals such as sodium or potassium, the alkaline earth metals such as ~ r~

calcium or with the ammonium cation. ~hen X is amino, the compounds can exist as the zwitterion or as an acid or hase salt. These salts are prepared by standard methods using a wide variety of nontoxic pharmaceutically acceptable acids and bases known in the art and are also considered as objects of this invention.
It will be recognized that because of the asymmetric ~-carbon atom in the 7-acetamido group of Formula I, optical isomers will exist. Racemic or resolved products are obtained depending upon whether a racemic or resolved side chain acid is used as an acylating agent.
The resolved side chain acids are readily obtained from the racemic compounds by resolution according to well known methods including fractional crystallization of a salt formed with an optically active acid or base. All of the isomers, including separated isomers and mixtures thereof, are included within the scope of this invention.
The compounds of Formula I have antibacterial activi-ty against both Gram-positive and Gram-negative organisms. Minimum inhibitory concentrations (MIC's) ranged from 0.2 to >200 ~g./ml. in in vitro testing These results are shown in Table I below for representa-tive compounds of this invention. In vivo mouse pro-tection data are given in Table 2. Compound numbers corresponding to structures are given in the experimental section.

5~3ZZ r r-l r~ o ~ o o 1-i ~ O
o o ~ ~~ ~ ~o . ~ ~ ~ o o ~ ~ o H ~ r--l ~ ~ ~1 0 r--l ~~I ~ (~ ~I t~l O O A A

HLt~ O O ~J ~'~ ~ OCOr--l ~)O ~1 0 H . 11~ O O . . . .O . . O . O :, H ~ ~ ~ D~J~1 ~O ~ ~ ~It~
r~ ~1 A A
._ _ u~
. . O 0~1 . . . O. . Il`) O~1 0 Ht`J t~l O O f~ D O~1~ ~ O O
H ~I r--l ~`J r~
A A A A
.._ ~1 0 0 ~ ~ In o n ~ o n o .
H . . O O . ~. ~1 0 . . O . O
_ ~ ~ t~ 9 0 ~
A ~1 ~ A r--l Lr) A r-l A
~ . :'~
. _ , :
l ~: , _ .. ~~ ~ t~ ~
,~ u~ ~ o o u~ o ~ ~ o ~ o -- .
O ~ ,~ o o r~~ ~ r` o ~ ~ u~ O ~ O
r,~ ~ ~I ~ ~(~J.. . (~. ~ ~ .
~I r~ 5)~1 ~ A~9 ~ A ~D A
.,1 ., .~ ~ .:
r~ ~ ~ O ~~~D ~ . . . O I O
~ L~ ~ ~I ,~ ~ O O I O
,_1 H H A A
1 1 ~ - : ' ~' .
E~ ~ D O O O O In In 15~ ~) O O I O
H O O Lr~ ~1 0 0 1 0 H r--l r~l ~ ~
H A A
:~
~ 9 o o In o u~ o ~ ~ o o I o H O O~15~ --1~1 U) O I O :~
H ~1~1 (`1 1 A A
_~
: `
In '~9 ~ In ~ u~ O
~ ,~ O O O~ ~ ~Lr) u~ o n HIJ') OO O~It') Lf)~ O. . r--l O . O
~ I . ~ O (~ ~ ` O N O

L~ ~ ~ ~ r~ ,~ U~ ~ ~ ~ :
~I N ~ r-l~D A ~`1 A A

CO
:: o o In ~ CO ~ .:
O O ~ r~
O O l_ ~ ~ r~
O ~ ~ ~ ~) ~ I` ' cj~ ~) r~
~~ ~ OC~ ~ ~ ~ ~ O
a) ~I N ~ ~ ~ ~ E-l r--l t~) U
X ?~ ~1 ~ O O ~ O
O X U~ N ~ O 5-~
m u~ X
h H ,~ r~ O F ~1 0 ~ O
O O ~ o Q Q
h ~ O
. . . ~ . . ~1 ~1 u~ ~ ~ F O F. S I

~8S~32Z

ED50 ln vivo (mg./kg.) Compound E. coli 12-140 ~leb. pneumo. 4200 s.c. p.o. s.c. p.o.
I 100 >200 II __ III 9.8,<3 78,50 <3,3 50,38 IV 5.2 35 <3,1.5 8.2,10 V 25 >200 >50 >50 ;-10 VI 25 >200 86 >200 VII 3 >50 1.1 31 VIII 1.1 >50 0.8,~0.78 39 IX 1 41 1.5,<3 71,50 Pharmaceutical compositions having antibacterial activity which comprise a pharmaceutical carrier and a compound of Formula I and methods of producing anti-bacterial activity by administering a compound of Formula :~
I to an animal in a nontoxic amount sufficient to produce said activity are also objects of this invention. The administration may be orally or by parenteral injection ~-such as subcutaneously, intramuscularly or intravenously.
The injection of suitably prepared sterile solutions or suspensions containing an efEective, nontoxic amount of the new cephalosporin compound is the preferred route of administration.
The col~pounds of Formula I are formulated and administered in the same manner as other cephalosporins in dosages of from 250 to 1000 mg. with the total daily dosage being from 1 to 6 g. The precise dosages are dependent upon the age and weight of the subject and on the infection being treated and can be determined by those g _ 5~3'2;~
:, skilled in the art based on the data disclosed herein com-pared with that available to the art attained with known cephalosporins.
The following examples illustrate the inven-` tion, but are not to be construed as limiting the scope thereof. Temperatures are in degrees Centigrade unless otherwise stated.

7-Amino-3-(4-carboxy-1,2,3-triazol-5-ylthiomethyl)-3-cephem-4-carboxylic acid To a solution of 4.6 g. (0.20 mol.) of sodium and 250 ml. of absolute ethanol was added under a nitrogen atmosphere 8.65 g. (0.05 mol.) of 5-amino-4-carbethoxy-1,2,3-thiadiazole. The reaction mixture was stirred one hour, 5 ml. of water was added and the mixture was re-fluxed for 12 hours. After cooling, the mixture was -~
filtered and the solid product was washed with ethanol and ether and dried to give 4-carboxy-1,2,3-triazole-5-thiol trisodium salt.
4-(Carboxy-1,2,3-triazole-5-thiol trisodium salt hydrate (4.4 g., 20 mmol.) was added to a stirred sus- -pension of 4.08 g. (15 mmol.) of 7-aminocephalosporanic acid in 30 ml. of water and 15 ml. of acetone. The reaction mixture was quickly acidified to pH 7.3 with dilute hydrochloric acid, then heated at 65C for 4.5 hours. The mixture was cooled (ice bath), brought to pH 2.4 with dilute hydrochloric acid and the precipitate was collected, washed with water and acetone and dried in vacuo over phosphorus pentoxide to give the title compound.

,; ~ 10 --z~

7-D-Mandelamido-3-(4-carboxy-1,2,3-triazol-5-ylthiomethyl)-,~ , . .............. . . .
~-cephem-4-carboxyllc acl~ ~VII
A solution of 4.73 g. (16.8 mmol.~ of D-O-dichloroacetylmandeloyl chloride in 15 ml. of dry acetone was added dropwise to a cooled (-15) solution of 3.0 g.
(8.4 mmol.) of 7-amino-3~(4-carboxy-1,2,3-triazol-5-ylthiomethyl)-3-cephem-4-carboxylic acid in a mixture of -70 ml. of 3% aqueous sodium bicarbonate and 70 ml. of acetone while maintaining the pH between 5.0 and 5.5 by addition of 10% aqueous sodium hydroxide. The reaction ;~
mixture was stirred at 25 for two hours, then extracted with ether. The aqueous layer was brought to pH 2.0 with 3N hydrochloric acid and the resulting mixture was extracted with ethyl acetate. The ethyl acetate solution was concentrated to about 20 ml. and added dropwise to a rapidly stirred mixture of 200 ml. of ether and 200 ml.
of petroleum ether (b.p. 30-60). The precipitate was collected and dissolved in ethyl acetate. Chloroform was added, the solution filtered, the filtrate evaporated to dryness, the residue dissolved in 50 ml. of dry methanol and the pH was adjusted to 10.0 by addition of 5% sodium methoxide in methanol at 5~. After 0.5 hour, the pH was adjusted to 7.0 by addition of 2-ethylhexanoic acid, the solution was filtered and the filtrate was diluted with ether to give a precipitate which was collected and dissolved in water. The aqueous solution was filtered and lyophilized to give 7-D-mandelamido-3-(4-carboxy-1,2,3-triazol-5-ylthiomethyl)-3-cephem-4-carboxylic acid disodium salt hydrate.

z ClgEll5N5O7s2 2 Na 3-5 H2 Calculated: 38.13~ C; 3.70% H; 11.70~ N
Found: 37.95% C; 3.05% H; 11.25% N
7-D-Mandelamido-3-(4~carboxy-1,2,3-triazol-5-ylthiomethyl)-3-cephem-4-carboxylic acid disodium salt is dissolved in a minimum amount of water to which chloro-form is added. While stirring, 3N hydrochloric acid is added until the solution is acidified to pH 2.5. The layers are separated, the aqueous phase is extracted with chloroform and the combined extracts are washed with water, dried (MgSO4) and evaporated to dryness to give the title compound. ;

7-(D-~-Aminophenylacetamido)-3-(4-carboxy-1,2,3-triazol-5-ylthiomethyl)-3-cephem-4-carboxylic acid (V) A solution of 5.05 g. (10 mmol.) of 7-(D-~-t--butoxycarbonylaminophenylacetamido)cephalosporanic acid and 2.84 g. (12.9 mmol.j of 4-carboxy-1,2,3-triazole-5-thiol trisodium salt in 75 ml. of pH 6.4 phosphate buffer solution was heated at 70 for 4.5 hours. The cooled reaction mixture was extracted with ethyl acetate. Fresh ethyl acetate was added and the solution was cooled and acidified to pH 2.0 with 3N hydrochloric acid~ The layers were separated and the aqueous phase was re-extracted with ethyl acetate. The combined extracts were dried (MgSO4) and evaporated to dryness to give a residue which was chromatographed on silica gel with 8:2:1 chloroform-isopropanol-formic acid as eluant to give 7-(D-~-t-butoxycarbonylaminophenylacetamido)-3-(4-carboxy-1,2,3-triazol-5-ylthiomethyl)-3-cephem-4-carboxylic acid.
7-(D-~-t-butoxycarbonylaminophenylacetamido)-2~

3-(4-carboxy-1,2,3-triazol-5-ylthiomethyl)~3-cephem-4-carboxylic acid wa$ stirred for 45 minutes at 5 in a mixture of 9 ml. of trifluoroacetic acid and 1 ml. of anisole. The mixture was evaporated to dryness and the residue was triturated with ether to give a solid which was dissolved in 125 ml. of water, treated with excess Amberlite IR-45 ion-exchange resin until the pH became constant and lyophilized to give the title compound.
ClgHl8N606s2 2-25 H20 Calculated: 42.97% C; 4.27% H; 15.83% N
Found: 42.99% C; 3.93% H; 15.79% N

7-Amino-3-(4-carbethoxy-1,2,3-triazol-5-ylthiomethyl)-3-cephem-4-carboxylic acid To a solution of 100 mg. (0.578 mmol.) of 5-amino-4-carbethoxy-1,2,3-thiadiazole in 4 ml. of absolute ethanol was added 79 mg. (1.16 mmo:L.) of sodium ethoxide.
The mixture was stirred at 25 for four hours, then 20 ml.
of ether was added and the resulting solid was collected and dried _ vacuo over phosphorus pentoxide to give

4-carbethoxy-1,2,3-triazole-5-thiol disodium salt hydrate.
4-Carbethoxy-1,2,3-triazole-5-thiol disodium salt hydrate (3.0 g., 13.8 mmol.) was added to a solution of 2.79 g. (10.2 mmol.) of 7-aminocephalosporanic acid ; and 1.72 g. (20.5 mmol.) of sodium bicarbonate in 40 ml.
of water and 10 ml. of acetone. The reaction mixture was acidified to pH 7.2 with glacial acetic acid then refluxed for 2.5 hours. The cooled mixture was acidified to pH 3.9 with 3N hydrochloric acid and the precipitated solid was collected, washed with water and acetone and dried (P 0 in vacuo) to give the title compound.

z%

7-D-Mandelamido-3-(4-carbethoxy-1,2,3-triazol-5-ylthio-methyl)-3-cephem-4-carboxylic acid When an equivalent amount of 7-amino-3-(4-carbethoxy-1,2,3-triazol-5-ylthiomethyl)-3-cephem-4-carboxylic acid is reacted with D-O-dichloroacetyl-mandeloyl chloride as described in the procedure of Example 2, the title compound is obtained as the final product.

7-(D~-Aminophenylacetamido)-3-(4-carbethoxy-1,2,3-triazol-5-ylthiomethyl)-3-cephem-4-carboxyllc acid (II) Substitution of 4-carbethoxy-1,2,3-triazole-5-; thiol disodium salt in the procedure of Example 3 in place of 4-carboxy-1,2,3-triazole-5-thiol trisodium salt ., gave 7-(D-~-t-butoxycarbonylaminophenylacetamido)-3-(4-carbethoxy-1,2,3-triazol-5-ylthiomethyl)-3-cephem-4-carboxylic acid, which upon treatment with trifluoro-acetic acid and anisole as described therein, gave the title compound.

i Calculated: 40.80% C; 4.75% H; 12.98% N
Found: 40.29% C; 4.02% H; 13.49% N

7-(D-~-Amino-4-hydroxyphenylacetamido)-3-(4-carbethoxy-1,2,3-triazol-5-ylthiome hyl)-3-cephem-4-carboxylic acid (I) When 5.31 g. (10.2 mmol.) of 7-(D-~-t-butoxy-carbonylamino-4-hydroxyphenylacetamido)cephalosporanic acid and 3.0 g. (11.5 mmol.) of 4-carbethoxy-1,2,3-triazole-5-thiol disodium salt were substituted in the procedure of Example 3 for 7-(D-~-t-butoxycarbonylamino-phenylacetamido)cephalosporanic acid and 4-carboxy-1,2,3-~5~3Z;2 triazole-5-thiol trisodium salt and the resulting 7-(D-~-t-~butoxycar~onylamino-4-hydroxyphenylacetamido~-3-(4-carbethoxy-1,2,3-triazol-5-ylthiomethyl~-3-cephem-4-carboxylic acid was treated with trifluoroacetic acid as described therein, the title compound was obtained.
C21H22N6O7s2 3 H2 Calculated: 42.85% C; 4.79% H; 14.28% N
Found: 43.30% C; 4.19% H; 13.84% N

7-Amino-3-(5-carboxymethyl-1,2,4-triazol-3-ylthiomethyl)-3-_phem-4-carboxylic acid 10% Aqueous sodium hydroxide (8 ml.) was added to a suspension of 5.4 g. (20 mmol.) of 7-amino-cephalosporanic acid and 5.5 g. (27 mmol.) of 3-carboxy-methyl-1,2,4-triazole-5-thiol disodium salt, prepared by addition of a sodium hydroxide solution to a solution of 3-carboxymethyl-1,2,4-triazole-5-thiol in aqueous ethanol.
The reaction mixture was refluxed for five hours and the ethanol was removed in vacuo. The aqueous residue was cooled and acidified to pH 2.8 with 3N hydro-chloric acid. The resulting solid was collected, washed with water and acetone and dried (P2O5 in vacuo) to give the title compound.

7-D-Mandelamido-3-(5-carboxymethyl-1,2,4-triazol-3-ylthio-methyl)-3-cephem-4-carboxylic acid (IX) 7-Amino-3-(5-carboxymethyl-1,2,4-triazol-3- -ylthiomethyl)-3-cephem-4-carboxylic acid (3.7 g., 10 ~mol.) and 5.6 g. (20 mmol~) of D-O-dichloroacetylmandeloyl chloride were reacted as described in the procedure of Example 2. The cooled reaction mixture was extracted with ether, the layers were separated and the pH of the 8Z~2 aqueous phase was adjusted to 2.0 by addition of 3N
hydrochloric acid. The xesulting mixture was extracted with ethyl acetate, the residual solid material was dis-carded and the organic solution was dried (MgSO~).
Addition of ether precipitated a solid which eas dissolved in cold 5% aqueous sodium carbonate. The solution was stirred for 20 minutes then acidified to pH 2.0 by addition of 3N hydrochloric acid and extracted with ethyl acetate.
The organic phase was dried (MgSO4) and evaporated to dryness to give a residue which was triturated with ethyl acetate to give the title compound.
c2OHl9N5o7s2 H2 Calculated: 45.88% C; 4.04% H; 13.38% N
Found: 46.03% C; 3.88% H; 12.94% N

7-(D-~-Aminophenylacetamido)-3-(5-carboxymethyl-1,2,4-triazol-3-ylthiomethyl)-3-cephem-4-earboxylic aeid (VIII) Substitution of an equivalent amount of 7-(D-~-t-butoxycarbonylaminophenylacetamido)cephalosporanic acid in the procedure of Example 8 gave 7-(D-~-_-butoxy-carbonylaminophenylacetamido)-3-(5-carboxymethyl-1,2,4-triazol-3-ylthiomethyl)-3-cephem-4-carboxylic acid which, upon treatment with trifluoroacetic acid as described in Example 3, gave the title compound.
C20H2oN6o6s2 2 H2 Calculated: 44.44% C; 4.48% H; 15.55~ N
Found: 44.49% C; 4.10% H; 15.28% N

7-(D-~-Amino-4-hydroxyphenylacetamido)-3-(5-carboxymethyl-1,2,4-triazol-3-ylthiomethyl)-3-cephem-4-carboxylic acid When 4.38 g. (8.4 mmol.) of 7-(D-~-t-butoxy-S~Z

carbonylamino~4-hydroxyphenylacetamido~cephalosporanic acid and 3-carboxymethyl-1,2,4-triazole-5-thiol were reacted as described in the procedure of Example 8 and the resultiny 7-(D-~-t-butoxycarbonylamino-4-hydroxyphenylacetamido)-3-(5-carboxymethyl-1,2,4-triazol-3-ylthiomethyl)-3-cephem-4-carboxylic acid was treated with trifluoroacetic acid as described therein, the title compound was obtained.
C20H2oN6o7s2 4 H2 Calculated: 40.53% C; 4,76% H; 14.18% N
Found: 39.98% C; 4.11% H; 14.14% N

7 Amino-3-(5-carbethoxymethyl-1,2,4-triazol-3-ylthio-methyl)-3-cephem-4-carboxylic acid Reaction of 3-carbethoxymethyl-1,2,4-triazole-

5-thiol sodium salt, prepared as described in Example 8, and 7-aminocephalosporanic acid according to the procedure described in Example 8 gives the title compound.

7-D-Mandelamido-3-(5-carbethoxymethyl-1,2,4-triazol-3-vlthiomethvl)-3-cephem-4-carboxylic acid .. ~ :
Substitution of an equivalent amount of 7-amino-3-(5-carbethoxymethyl-1,2,4-triazol-3-ylthiomethyl)-3-cephem-4-carboxylic acid in the procedure of Example 2 for 7-amino-3-(4-carboxy-1,2,3-triazol-5-ylthiomethyl)-3-cephem-4-carboxylic acid gives the title compound.

7-(D-~-Aminophenylacetamido)-3-(5-carbethoxymethyl-1,2,4-triazol-3-ylthiomethyl)-3-cephem-4-carboxylic acid (III) The title compound was prepared by use of 3-carbethoxymethyl-1,2,4-triazole-5-thiol sodium salt in place of 3-carboxymethyl-1,2,4-triazole-5-thiol disodium salt and 7-(D-~-t-butoxycarbonylaminophenylacetamido)cephalo-sporanic acid in place of 7-aminocephalosporanic acid in the procedure of Example 8 with subsequent treatment of the 7-(D-u-t-butoxycarbonylaminophenylacetamido~-3-(5-carbethoxy-methyl-1,2,4-triazol-3-ylthiomethyl~-3-cephem-4-carboxylic acid with trifluoroacetic acid as described abo~e.
C22~I24N6O6s2 3.5 H2 Calculated: 44.36% C; 5.25% H; 14.11% N
Found: 44.03% C; 4.47% H; 14.03% N
E ~ ~LE 15 7-(D-~-Amino-4-hydroxyphenylacetamido)-3-(4-carbamoyl- -1,2,3-triazol-5-ylthiomethyl)-3-cephem-4-carboxylic acid (IV) -Reaction of 7-(D-~-t-butoxycarbonylamino-4-hydroxyphenylacetamido)cephalosporanic acid, 4-carbamoyl-1,2,3-triazole-5-thiol sodium salt prepared as described in Example 8 and an excess amount of-sodium bicarbonate solution according to the procedure described in Example 3 with subsequent treatmentof the 7-(D-~-t-butoxy-carbonylamino-4-hydroxyphenylacetamido)-3-(4-carbamoyl-1, 2,3-triazol-5-yl-thiomethyl)-3-cephem-4-carboxylic acid thus formed with trifluoroacetic acid gave the title compound.
Clg~IlgN7o6s2 2 H2O
Calculated: 42.14% C; 4.28% H; 18.10% N
Found: 42.62% C; 3.97% H; 17.30% N
EXA~LE 16 7-Amino-3-(4-carboxymethyl-1,2,4-triazol-3-ylthiomethyl)-3-cephem-4-carboxylic acid To a stirred solution of 2.9 g. (0.02 mol.) of ethyl ~-isothiocyanoacetate in 20 ml. of dioxane was added dropwise a solution of 1.20 g. (0.02 mol.) of formyl-hydrazine in 35 ml. of dioxane. The reaction mixture was stirred for one hour to give 1-carbethoxymethyl-4-formyl-thiosemicarbazide.
l-Carbethoxymethyl~4-formylthiosemicarbazide (0.50 g., 2.44 mmol.) was dissolved in 5 ml. of ethanol and 5 m].
(2 equivalents) of aqueous potassium hydroxide solution was added. The reaction mixture was refluxed for five hours then cooled, acidified to pH 2.0 with 6N sulfuric acid and evaporated to dryness. The residue was extracted with acetone to give 4-carboxymethyl-1,2,4-triazole-3-thiol.
Substitution of an equivalent amount of 4-carboxymethyl-1,2,4-triazole-3-thiol in the procedure of Example 8 in place of 3-carboxymethyl-1,2,4-triazol-5-thiol gives the title compound.
EX~PLE 17 7-D-Mandelamido-3-(4-carboxymethyl-1,2,4-triazol-3-ylthio-methyl)-3-cephem-4-carboxylic acid When 7-amino-3-(4-carboxymethyl-1,2,4-triazol-3-ylthiomethyl)-3-cephem-4-carboxylic acid is used in the procedure of Example 2 in place of 7-amino-3-(4-carboxy-1,2,3-triazol-5-ylthiomethyl)-3-cephem-4-carboxylic acid, the title compound is obtained.

7-(D-~-Aminophenylacetamido)-3-(4-carboxymethyl-1,2,4-triazol-3-ylthiomethyl)-3-cephem-4-carboxylic acid _ Reaction of 7-(D-~-t-butoxycarbonylamino-phenylacetamido)cephalosporanic acid and 4-carboxymethyl-1,2,4-triazole-3-thiol disodium salt according to the procedure of Example 8 followed by treatment of the 7-(D-~-t-butoxycarbonylaminophenylacetamido)-3-(4-carboxy-methyl-1,2,4-triazol-3-ylthiomethyl)-3-cephem-4-carboxylic acid thus obtained with trifluoroacetic acid as described ~.V~
in Example 3, gives the title compound.

To a solution of 1.725 g. (75 mmol.) of sodium in 50 ml. of absolute ethanol is added 6.0 g. (45 mmol.) of benzylazide and 12.3 g. (50 mmol.) of diethyl 2-(carbo-methoxyethyl)malonate. The reaction mixture is refluxed for 12 hours, then cooled and evaporated to dryness.
Water (100 ml.~ is added and the mixture is heated while maintaining the pH at 12. The aqueous mixture is extracted with ethyl acetate and the aqueous phase is `
acidified with 3N hydrochloric acid and extracted with ethyl acetate. The ethyl acetate solution is evaporated to dryness to give l-benzyl-4-(2-carboxyethyl)-5-hydroxy- -1,2,3-triazole.
l-Benzyl-4-(2-carboxyethyl)-5-hydroxy-1,2,3-triazole (5.0 g., 0.02 mol.) is suspended in 300 ml. of ethanol. Anhydrous hydrogen chloride gas is bubbled into the suspension for 30 minutes and the mixture is then heated an a steam bath for 20 minutes. The solution is cooled and evaporated to dryness to give a residue which is dissolved in 200 ml. of ethyl acetate. The ethyl acetate solution is washed with water, dried (MgSO4) and evaporated to dryness. Trituration with hexane containing a little acetone gives l-benzyl-4-(2-carbethoxyethyl)-5-hydroxy-1,2,3-triazole. `~
To a solution of l.0 g. (4 mmol.) of 1-benzyl-4-(2-carbethoxyethyl)-5 hydroxy-1,2,3-triazole in 2 ml. of phosphorus oxychloride is added 0.5 g. (5 mmol.) of phosphorus pentachlorideO The reaction mixture is stirred at 25 for two hours, then warmed on a steam bath for 10 minutes and evaporated to dryness. The residue 8;~;~

is dissolved in 0.5 ml. of phosphorus oxychloride and the solution is heated to reflux for 30 minutes, cooled and evaporated to dryness to give l-benzyl-4-(2-carbethoxyethyl~-5-chloro-1,2,3-triazole.
A solution of 0.54 g. (0.01 mol.) of sodium methoxide in 50 ml. of absolute ethanol is saturated with hydrogen sulfide. 1-Benzyl-4-(2-carbethoxyethyl)-5-chloro-1,2,3-triazole (2.9 g., 0.01 mol.) is added and the reaction mixture is refluxed for 24 hours~ The cooled mixture is evaporated to dryness to give 1-benzyl-4-(2-carboxyethyl)-1,2,3-triazole-5-thiol sodium salt.
Acidification of an aqueous solution of the triazole thiol salt as previously described gives l-benzyl-4-(2-carboxy-ethyl)-1,2,3-triazole-5-thiol.
l-Benzyl-4-carboxyethyl 1,2,3-triazole-5-thiol (1.1 g., 4 mmol.) is suspended in 50 ml. of anhydrous liquid ammonia and sodium is added until a permanent blue color results. The reaction mixture is allowed to stir for 40 minutes then allowed to warm to ambient temperature while the ammonia evaporates. The residue ~;~
is triturated with ether and the solid formed is collected and dissolved in water. The aqueous solution is acidified and extracted with ethyl acetate. The extract is dried (MgS04) and evaporated to dryness to give 4-(2-carboxyethyl)-1,2,3-triazol-5-thiol.
4-(2-Carboxyethyl)-1,2,3-triazol-5-thiol is esterified as described hereinabove to give 4-(2-carbethoxy-ethyl)-1,2,3-triazol-5-thiol.
~hen an equivalent amount of 4-(2-carboxyethyl)-1,2,3-triazol-5-thiol disodium salt, prepared as in Example 8, is reac-ted with 7-aminocephalosporanic acid 8~Z~

by the procedure of Example 8, 7-amino-3-[4-(2-carboxyethyl)-1,2,3-triazol-5-ylthiomethyl]-3-cephem-4-carboxylic acid is obtained.
In like manner, substitution of 4-~2-carbethoxy-ethyl)-1,2,3-triazole-5-thiol disodium salt, prepared as previously described, in the procedure of Example 4 for 4-carbethoxy-1,2,3-triazole-5-thiol disodium salt gives 7-amino-3=[4-(2-carbethoxyethyl)-1,2,3-triazol-5-ylthiomethyl]-3-cephem-4-carboxylic acid as the product.
EX~MPLE 20 Use of equivalent amounts of diethyl 2-carbethoxy-methylmalonate and diethyl 2-(carbethoxypropyl)malonate, respectively, in the procedure of Example 19 in place of diethyl 2-(carbomethoxyethyl)malonate, followed by the -~steps of ester hydrolysis, chlorination, sulfide displace-ment and debenzylation described therein gives 4-carboxy- .methyl-1,2,3~triazole-5-thiol and 4-(3-carboxypropyl)-1,2,3-triazole-5-thiol.
EsteriEication of 4-carboxymethyl-1,2,3-triazole-5-thiol and 4-(3-carboxypropyl)-1,2,3-triazole-5-thiol as described in Example 19 gives, respectively, 4-carbethoxy-methyl-1,2,3-triazole-5-thiol and 4-(3-carbethoxypropyl)-1,2, 3-triazole-5-thiol.
Use of 4-carboxymethyl-1,2,3-triazole-5-thiol disodium salt, 4-(3-carboxypropyl)-1,2,3-triazole-5-thiol disodium salt, 4-carbethoxymethyl-1,2,3-triazole-5-thiol sodium salt or 4-(3-carbethoxypropyl)-1,2,3-triazole-5-thiol sodium salt, all prepared as described in Example 8, in the procedure of Example 8 in place of 3-carboxymethyl-1,2,4- ;~
triazole-5-thiol disodium salt, gives the following 7-amino-3-(substituted triazolyl)thiomethyl cephalosporin compounds:

:'.

zz 7-amino-3-~4-carboxymethyl-1,2,3-triazol-5-ylthio-methyl~-3-cephem-4-carb~xyli~c acid 7-amin~ 3-14-(3-carboxypropyl~-1,2,3-triazol-5-ylthiomethyl]-3-cephem-4-carboxylic acid 7-amino-3-(4-carbethoxymethyl-1,2,3-triazol-5-ylthiomethyl)-3-cephem-4-carboxylic acid 7-amino-3-[4-(3-carbethoxypropyl)-1,2,3-triazol-5-ylthiomethyl]-3-cephem-4-carboxylic acid.

An injectable pharmaceutical composition is formed by adding sterile water or sterile saline solution (2 ml.) to 500 mg. of 7-D-mandelamido-3-(5-carboxymethyl-1,2,4-triazol-3-ylthiomethyl)-3-cephem-4-carboxylic acid, sodium salt.
Pharmaceutical compositions of the other anti-bacterial compounds disclosed above may be formulated in a similar manner.

~ tablet or capsule is formed from 500 mg. of 7- -~
(D-~-amino-4-hydroxyphenylacetamido)-3-(4-carbamoyl-1,2,3-triazol-5-ylthiomethyl)-3-cephem-4-carboxylic acid, 250 mg.
of lactose and 75 mg. of magnesium stearate.
Tablets or capsules of the other antibacterial compounds disclosed above may beformulated in a similar manner.

-.:

Claims

The embodiments of the invention in which an exclusive pro-perty or privilege is claimed are defined as follows:

1. A process for preparing a compound of the formula:

in which:
R1 is phenyl or phenyl substituted with hydroxy;
X is amino or hydroxy; and Het is 1,2,4-triazolyl or 1,2,3-triazolyl each of which is substituted with -(CHR2)nCoR3, where R is hydrogen n is zero to six and R3 is hydroxy, lower alkoxy, amino, lower alkylamino or di(lower)alkylamino, or a non-toxic pharmaceutically acceptable salt thereof, comprising acylating a compound of the formula:

where Het, R2, R3 and n are defined as above and R4 is hydrogen or a protecting ester group, with phenyl or phenyl substituted with hydroxy acetic acid, suitably activated and protected as necessary, or reacting a compound of the formula:

where R1 and X are defined as above, suitably protected as necessary, with a compound of the formula:

Het-SH or a salt thereof where Het is defined as above, then acidifying when necessary, removing the protective group(s) when necessary and optionally converting the product to a non-toxic pharmaceutically acceptable salt.

2. A process as claimed in claim 1 in which Het is 1,2,3-triazol-5-y1 or 1,2,4-triazol-3-y1, and n is zero to four.

3. A process as claimed in claim 2 in which R1 is phenyl or 4-hydroxyphenyl and R3 is hydroxy, lower alkoxy or amino.

4. A process as claimed in claim 1 in which X is hydroxy.

5. A process as claimed in claim 1 in which X is amino.

6. A process for preparing 7-mandelamido-3-(4-carboxy-1,2,3-triazol-5-ylthiomethyl)-3-cephem-4-carboxylic acid comprising acylating 7-amino-3-(,4-carboxy-1,2,3-triazol-5-ylthiomethyl)-3-cephem -4-carboxylic acid with O-dichloroacetylmandeloyl chloride and then removing the protective group.

7. A process for preparing 7-(.alpha.-aminophenylacet-amido)-3-(4-carboxy-1,2,3-triazol-5-ylthiomethyl)-3-cephem-4-carboxylic acid comprising reacting 7-(.alpha.-t-butoxycarbonyl-aminophenylacetamido)cephalosporanic acid with 4-carboxy-1,2, 3-triazole-5-thiol trisodium salt than acidifying and re-moving the protective group with trifluoroacetic acid.

8. A proeess for preparing 7-(.alpha.-aminophenylacet-amido)-3-(4-carbethoxy-1,2,3-triazol-5-ylthiomethyl)-3-cephem-4-carboxylic acid comprising reacting 7-(.alpha.-t-butoxycarbonylaminophenylacetamido)cephalosporanic acid with 4-carbethoxy-1,2,3-triazole-5-thiol disodium salt then acidifying and removing the protective group with tri-fluoroacetic acid.

9. A process for preparing 7-(.alpha.-amino-4-hydroxy-phenylacetamido)-3-(4-carbethoxy-1,2,3-triazol-5-ylthio-methyl)-3-cephem-4-carboxylic acid comprising reacting 7-(.alpha.-t-butoxycarbonylamino-4-hydroxyphenylacetamido)cephalo-sporanic acid with 4-carbethoxy-1,2,3-triazole-5-thiol disodium salt then acidifying and removing the protective group with trifluoroacetie acid.

10. A process for preparing 7-mandelamido-3-(5-carboxymethyl-1,2,4-triazol-3-ylthiomethyl)-3-cephem-4-carboxylic acid comprising acylating 7-amino-3-(5-carboxy-methyl-1,2,4-triazol-3-ylthiomethyl)-3-cephem-4-carboxylic acid with O-dichloroacetylmandeloyl chloride and then remov-ing the protective group.

11. A process for preparing 7-(.alpha.-aminophenylacet-amido)-3-(5-carboxymethyl-1,2,4-triazol-3-ylthiomethyl)-3-cephem-4-carboxylic acid comprising reacting 7-(.alpha.-t-butoxy-carbonylaminophenylacetamido) cephalosporanic acid with 3-carboxymethyl-1,2,4-triazole-5-thiol disodium salt then acidifying and removing the protective group with trifluoro-acetic acid.

12. A process for preparing 7-(.alpha.-amino-4-hydroxy-phenylacetamido)-3-(5-carboxymethyl-1,2,4-triazol-3-ylthio-methyl)-3-cephem-4-carboxylic acid comprising reacting 7-(.alpha.-t-butoxycarbonylamino-4-hydroxyphenylacetamido)cephalosporanic acid with 3-carboxymethyl-1,2,4-triazole-5-thiol disodium salt then acidifying and removing the protective group with tri-fluoroacetic acid.

13. A process for preparing 7-(.alpha.-aminophenylacet-amido)-3-(5-carbethoxymethyl-1,2,4-triazol-3-ylthiomethyl)-3-cephem-4-carboxylic acid comprising reacting 7-(.alpha.-t-butoxy-carbonylaminophenylacetamido)cephalosporanic acid with 3-carbethoxymethyl-1,2,4-triazole-5-thiol sodium salt then acidifying and removing the protective group with trifluoro-acetic acid.

14. A process for preparing 7-(.alpha.-amino-4-hydroxy-phenylacetamido)-3-(4-carbamoyl-1,2,3-triazol-5-ylthiomethyl)-3-cephem-4-carboxylic acid comprising reacting 7-(.alpha.-t-butoxy-carbonylamino-4-hydroxyphenylacetamido)cephalosporanic acid with 4-carbamoyl-1,2,3-triazole-5-thiol sodium salt then acidifying and removing the protective group with trifluoro-acetic acid.

15. A compound of the formula:

in which:
R1 is phenyl or phenyl substituted with hydroxy;
X is amino or hydroxy; and Het is 1,2,4-triazolyl or 1,2,3-triazolyl each of which is substituted with -(CHR2)nCOR2, where R2 is hydrogen n is zero to six and R3 is hydroxy, lower alkoxy, amino, lower alkylamino or di(lower)alkyl-amino, or a non-toxic pharmaceutically acceptable salt thereof, when prepared by the process of claim 1 or its obvious chemical equivalent.

16. A compound of claim 15 in which Het is 1,2,3-triazol-5-y1 or 1,2,4-triazol-3-y1, and n is zero to four, when prepared by the process of claim 2 or its obvious chemical equivalent.

17. A compound of claim 15 in which Het is 1,2,3-triazol-5-y1 or 1,2,4-triazol-3-y1, n is zero to four, R1 is phenyl or 4-hydroxyphenyl and R3 is hydroxy, lower alkoxy or amino, when prepared by the process of claim 3 or its obvious chemical equivalent.

18. A compound of claim 15 in which X is hydroxy, when prepared by the process of claim 4 or its obvious chemical equivalent.

19. A compound of claim 15 in which X is amino, when prepared by the process of claim 5 or its obvious chemical equivalent.

20. The compound 7-mandelamido-3-(4-carboxy-1,2,3-triazol-5-ylthiomethyl)-3-cephem-4-carboxylic acid, when pre-pared by the process of claim 6 or its obvious chemical equivalent.

21. The compound 7-(.alpha.-aminophenylacetamido)-3-(4-carboxy-1,2,3-triazol-5-ylthiomethyl)-3-cephem-4-carboxylic acid, when prepared by the process of claim 7 or its obvious chemical equivalent.

22. The compound 7-(.alpha.-aminophenylacetamido)-3-(4-carbethoxy-1,2,3-triazol-5-ylthiomethyl)-3-cephem-4-carboxylic acid, when prepared by the process of claim 8 or its obvious chemical equivalent.

23. The compound 7-(.alpha.-amino-4-hydroxyphenyl-acetamido)-3-(4-carbethoxy-1,2,3-triazol-5-ylthiomethyl)-3-cephem-4-carboxylic acid, when prepared by the process of claim 9 or its obvious chemical equivalent.

24. The compound 7-mandelamido-3-(5-carboxymethyl-1,2,4-triazol-3-ylthiomethyl)-3-cephem-4-carboxylic acid, when prepared by the process of claim 10 or its obvious chemical equivalent.

25. The compound 7-(.alpha.-aminophenylacetamido)-3-(5-carboxymethyl-1,2,4-triazol-3-ylthiomethyl)-3-cephem-4-carboxylic acid, when prepared by the process of claim ll or its obvious chemical equivalent.

26. The compound 7-(.alpha.-amino-4-hydroxyphenyl-acetamido)-3-(5-carboxymethyl-1,2,4-triazol-3-ylthiomethyl)-3-cephem-4-carboxylic acid, when prepared by the process of claim 12 or its obvious chemical equivalent.

27. The compound 7-(.alpha.-aminophenylacetamido)-3-(5-carbethoxymethyl-1,2,4-triazol-3-ylthiomethyl)-3-cephem-4-carboxylic acid, when prepared by the process of claim 11 or its obvious chemical equivalent.

28. The compound 7-(.alpha.-amino-4-hydroxyphenyl-acetamido)-3-(4-carbamoyl-1,2,3-triazol-5-ylthiomethyl)-3-cephem-4-carboxylic acid, when prepared by the process of claim 14 or its obvious chemical equivalent.