CA1075180A - Process of manufacturing enzyme preparation rich in lipase - Google Patents
Process of manufacturing enzyme preparation rich in lipaseInfo
- Publication number
- CA1075180A CA1075180A CA277,779A CA277779A CA1075180A CA 1075180 A CA1075180 A CA 1075180A CA 277779 A CA277779 A CA 277779A CA 1075180 A CA1075180 A CA 1075180A
- Authority
- CA
- Canada
- Prior art keywords
- tissue
- hydrocarbon
- phase
- acetone
- enzyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 102000004882 Lipase Human genes 0.000 title claims abstract description 8
- 108090001060 Lipase Proteins 0.000 title claims abstract description 8
- 239000004367 Lipase Substances 0.000 title claims abstract description 8
- 235000019421 lipase Nutrition 0.000 title claims abstract description 8
- 102000004190 Enzymes Human genes 0.000 title claims description 24
- 108090000790 Enzymes Proteins 0.000 title claims description 24
- 238000000034 method Methods 0.000 title claims description 21
- 238000002360 preparation method Methods 0.000 title claims description 15
- 238000004519 manufacturing process Methods 0.000 title claims description 3
- 239000000203 mixture Substances 0.000 claims abstract description 19
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 11
- 210000004923 pancreatic tissue Anatomy 0.000 claims abstract description 10
- 238000009835 boiling Methods 0.000 claims abstract description 8
- 150000008282 halocarbons Chemical class 0.000 claims abstract description 6
- 150000004945 aromatic hydrocarbons Chemical class 0.000 claims abstract description 5
- 150000001338 aliphatic hydrocarbons Chemical class 0.000 claims abstract description 4
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 59
- 239000000839 emulsion Substances 0.000 claims description 28
- 229940088598 enzyme Drugs 0.000 claims description 23
- 239000012071 phase Substances 0.000 claims description 20
- 239000000463 material Substances 0.000 claims description 17
- 208000035404 Autolysis Diseases 0.000 claims description 13
- 206010057248 Cell death Diseases 0.000 claims description 13
- 238000005238 degreasing Methods 0.000 claims description 13
- 230000028043 self proteolysis Effects 0.000 claims description 12
- 239000012141 concentrate Substances 0.000 claims description 11
- 239000002904 solvent Substances 0.000 claims description 11
- 210000001519 tissue Anatomy 0.000 claims description 10
- 230000000694 effects Effects 0.000 claims description 9
- 239000011877 solvent mixture Substances 0.000 claims description 9
- 229930195733 hydrocarbon Natural products 0.000 claims description 8
- 239000004215 Carbon black (E152) Substances 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 6
- 150000002430 hydrocarbons Chemical class 0.000 claims description 6
- 239000002244 precipitate Substances 0.000 claims description 6
- 239000003791 organic solvent mixture Substances 0.000 claims description 4
- 238000001556 precipitation Methods 0.000 claims description 3
- 239000008346 aqueous phase Substances 0.000 claims description 2
- 125000004432 carbon atom Chemical group C* 0.000 claims description 2
- 229940079919 digestives enzyme preparation Drugs 0.000 claims description 2
- 239000003960 organic solvent Substances 0.000 claims 2
- 210000000496 pancreas Anatomy 0.000 description 14
- 239000007787 solid Substances 0.000 description 11
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 9
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 9
- 235000008504 concentrate Nutrition 0.000 description 9
- 235000019626 lipase activity Nutrition 0.000 description 8
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 6
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 6
- 238000005406 washing Methods 0.000 description 6
- AMQJEAYHLZJPGS-UHFFFAOYSA-N N-Pentanol Chemical compound CCCCCO AMQJEAYHLZJPGS-UHFFFAOYSA-N 0.000 description 5
- 235000013339 cereals Nutrition 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 238000003756 stirring Methods 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 210000004907 gland Anatomy 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 241000219739 Lens Species 0.000 description 3
- 235000014647 Lens culinaris subsp culinaris Nutrition 0.000 description 3
- 108010019160 Pancreatin Proteins 0.000 description 3
- 239000000498 cooling water Substances 0.000 description 3
- 229910052736 halogen Inorganic materials 0.000 description 3
- -1 halogen hydrocarbons Chemical class 0.000 description 3
- 229940055695 pancreatin Drugs 0.000 description 3
- 238000011084 recovery Methods 0.000 description 3
- XSTXAVWGXDQKEL-UHFFFAOYSA-N Trichloroethylene Chemical group ClC=C(Cl)Cl XSTXAVWGXDQKEL-UHFFFAOYSA-N 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- ZSIAUFGUXNUGDI-UHFFFAOYSA-N hexan-1-ol Chemical compound CCCCCCO ZSIAUFGUXNUGDI-UHFFFAOYSA-N 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- UBOXGVDOUJQMTN-UHFFFAOYSA-N trichloroethylene Natural products ClCC(Cl)Cl UBOXGVDOUJQMTN-UHFFFAOYSA-N 0.000 description 2
- GHPYJLCQYMAXGG-WCCKRBBISA-N (2R)-2-amino-3-(2-boronoethylsulfanyl)propanoic acid hydrochloride Chemical compound Cl.N[C@@H](CSCCB(O)O)C(O)=O GHPYJLCQYMAXGG-WCCKRBBISA-N 0.000 description 1
- BOSAWIQFTJIYIS-UHFFFAOYSA-N 1,1,1-trichloro-2,2,2-trifluoroethane Chemical compound FC(F)(F)C(Cl)(Cl)Cl BOSAWIQFTJIYIS-UHFFFAOYSA-N 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 235000014676 Phragmites communis Nutrition 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 125000001309 chloro group Chemical group Cl* 0.000 description 1
- 102000038379 digestive enzymes Human genes 0.000 description 1
- 108091007734 digestive enzymes Proteins 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000002657 fibrous material Substances 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 230000000762 glandular Effects 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 238000005469 granulation Methods 0.000 description 1
- 230000003179 granulation Effects 0.000 description 1
- 150000002367 halogens Chemical class 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 239000010802 sludge Substances 0.000 description 1
- 239000011343 solid material Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/94—Pancreatin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
- C12N9/20—Triglyceride splitting, e.g. by means of lipase
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/814—Enzyme separation or purification
- Y10S435/816—Enzyme separation or purification by solubility
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicinal Preparation (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE2620289A DE2620289C2 (de) | 1976-05-07 | 1976-05-07 | Herstellung eines lipasereichen Enzympräparates |
| DE19772716719 DE2716719C2 (de) | 1977-04-15 | 1977-04-15 | Herstellung eines lipasereichen Enzympräparates |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1075180A true CA1075180A (en) | 1980-04-08 |
Family
ID=25770433
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA277,779A Expired CA1075180A (en) | 1976-05-07 | 1977-05-05 | Process of manufacturing enzyme preparation rich in lipase |
Country Status (15)
| Country | Link |
|---|---|
| US (1) | US4088539A (en:Method) |
| JP (1) | JPS52154592A (en:Method) |
| AT (1) | AT358723B (en:Method) |
| AU (1) | AU508908B2 (en:Method) |
| CA (1) | CA1075180A (en:Method) |
| CH (1) | CH635366A5 (en:Method) |
| DK (1) | DK197477A (en:Method) |
| ES (1) | ES458684A1 (en:Method) |
| FR (1) | FR2350357A1 (en:Method) |
| GB (1) | GB1533537A (en:Method) |
| GR (1) | GR63224B (en:Method) |
| MX (1) | MX4738E (en:Method) |
| NL (1) | NL7704942A (en:Method) |
| PT (1) | PT66519B (en:Method) |
| SE (1) | SE7705243L (en:Method) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0115023B1 (de) | 1982-12-30 | 1988-07-27 | Nordmark Arzneimittel GmbH | Verfahren zur Gewinnung von Pankreatin |
| SE454566B (sv) * | 1984-04-24 | 1988-05-16 | Lars G I Hellgren | Farmaceutisk komposition innehallande en verksam mengd av vattenlosliga proteinaser som extraherats fran ett vattenlevande djur valt av ordningen euphausiaceae eller slektet mallotus |
| US7153504B2 (en) * | 2004-07-30 | 2006-12-26 | Can Technologies, Inc. | Stabilized pancreas product |
| CA2607004C (en) | 2005-05-05 | 2016-01-26 | Sensient Flavors Inc. | Production of beta-glucans and mannans |
| JP4883703B2 (ja) * | 2007-07-31 | 2012-02-22 | カヤバ工業株式会社 | 複筒型緩衝器 |
| JP7139637B2 (ja) | 2018-03-19 | 2022-09-21 | 株式会社リコー | 画像処理装置及び投影システム |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1047422A (en) * | 1974-02-21 | 1979-01-30 | Hans Betzing | Process of manufacturing enzyme preparation rich in lipase |
-
1977
- 1977-05-03 US US05/793,322 patent/US4088539A/en not_active Expired - Lifetime
- 1977-05-03 MX MX775710U patent/MX4738E/es unknown
- 1977-05-04 JP JP5082577A patent/JPS52154592A/ja active Granted
- 1977-05-05 CH CH564477A patent/CH635366A5/de not_active IP Right Cessation
- 1977-05-05 GR GR53377A patent/GR63224B/el unknown
- 1977-05-05 NL NL7704942A patent/NL7704942A/xx not_active Application Discontinuation
- 1977-05-05 DK DK197477A patent/DK197477A/da not_active Application Discontinuation
- 1977-05-05 CA CA277,779A patent/CA1075180A/en not_active Expired
- 1977-05-05 SE SE7705243A patent/SE7705243L/xx not_active Application Discontinuation
- 1977-05-05 GB GB18930/77A patent/GB1533537A/en not_active Expired
- 1977-05-05 AU AU24931/77A patent/AU508908B2/en not_active Expired
- 1977-05-05 PT PT66519A patent/PT66519B/pt unknown
- 1977-05-05 FR FR7713690A patent/FR2350357A1/fr active Granted
- 1977-05-05 ES ES458684A patent/ES458684A1/es not_active Expired
- 1977-05-05 AT AT319577A patent/AT358723B/de not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5632913B2 (en:Method) | 1981-07-30 |
| CH635366A5 (de) | 1983-03-31 |
| NL7704942A (nl) | 1977-11-09 |
| MX4738E (es) | 1982-08-25 |
| FR2350357B1 (en:Method) | 1980-03-07 |
| ATA319577A (de) | 1980-02-15 |
| PT66519A (en) | 1977-06-01 |
| DK197477A (da) | 1977-11-08 |
| JPS52154592A (en) | 1977-12-22 |
| AT358723B (de) | 1980-09-25 |
| US4088539A (en) | 1978-05-09 |
| GB1533537A (en) | 1978-11-29 |
| ES458684A1 (es) | 1978-11-16 |
| GR63224B (en) | 1979-10-10 |
| SE7705243L (sv) | 1977-11-08 |
| FR2350357A1 (fr) | 1977-12-02 |
| AU2493177A (en) | 1978-11-09 |
| AU508908B2 (en) | 1980-04-03 |
| PT66519B (en) | 1978-10-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKEX | Expiry |