BRPI1013475A2 - INSETICIDATED AND LARVICIDE EMULSIONABLE CONCENTRATE, PROCEDURE FOR PREPARING AN INSETICIDES AND LARVICIDE EMULSIBLED CONCENTRATE, INSETICIDATED AND LARVICIDE COMPOSITION READY FOR USE, USE OF AN INSETICIDES AND LARVICIDES AND METHOD CONTROLS - Google Patents

Abstract

EMULSIONABLE INSECTICIDE AND LARVICIDE CONCENTRATE, PROCEDURE FOR THE PREPARATION OF AN INSECTICIDE AND LARVICIDE EMULSIONABLE CONCENTRATE, INSECTICIDE AND LARVICIDE COMPOSITION READY FOR USE, USE OF AN INSECTICIDE AND LARVICIDE COMPOSITION AND A CONTROLLED METHOD OF TREATMENT AND A CONTROLLED METHOD OF CONTENT contains pyrethroid insecticidal compounds in combination with juvenile IGR larvicides and excipients selected from at least one surfactant, an anti-evaporator, an anti-UV protector, an antioxidant, an antifoam and an appropriate organic solvent. The emulsion of the concentrate, preferably in water, is spread by means of environmental or surface spraying to control diptera in general and mosquitoes, such as vectors of diseases such as dengue, yellow fever and malaria in particular.

Description

“INSETICIDATED AND LARVICID EMULSIBLE CONCENTRATE, PROCEDURE FOR PREPARING AN INSETICIDATED AND LARVICIDE EMULSIONABLE CONCENTRATE, READY FOR USE, USE OF AN INSETICIDAL AND LARVICID COMPOSITION OF THE PRESENT INTEXT AND CONTROL OF THE INVENTION emulsifiable concentrate compositions for spatial application with insecticidal and larvicidal activity comprising at least one pyrethroid compound and pyriproxyfen as active compounds. The compositions of the invention have insecticidal and larvicidal activity, and are compatible for space use with water as a vehicle. Likewise, the present invention relates to a procedure for preparing the compositions and a method of controlling dengue, yellow fever and malaria mosquito vectors.

Background of the Invention Insecticides commonly called pyrethroids are currently widely used in pest control for their excellent selectivity. In other words, they have high insect pest toxicity, although low in mammals (Naumann K. 1990, Synthetic Pyrethroid Insecticides, Chemistry and Patents, Springer Verlag, Berlin; Ruigt SF 1985, Pyrethroids, pp. 183-262. G. Kerkut and L. Gilbert (editors) Comprehensive Insect Physiology, Biochemistry and Pharmacology, Vol. 12, Pergamon, Oxford, United Kingdom).

Pyrethroids are excellent insecticides for the control of adult flying forms of Diptera in general and mosquitoes in particular (Pest and Vector Control, H. van Emden and M. Service, Cambridge University Press, Cambridge, UK, 2004). Argentine Patent No. AR004978B1, which is incorporated herein by reference, describes a method of enrichment in the permethrin cis isomer, and its separation as a crystalline solid. Similarly, published Argentine patent application No. AR025747A1, incorporated herein by reference, discloses an emulsifiable cis-permethrin insecticide concentrate, a procedure for its preparation and use. This product has been shown to be effective in the laboratory and in the field to control adult Aedes aegypti dengue vector mosquitoes in space treatments. ("Laboratory and sca / ed up evaluation of Cis-permethrin applied as a new Ultra Low Volume formulation against Aedes aegypti (Diptera: culicidae) '' Emilia Seccacini, Masuh Héctor, Licastro Susana A. de and Zerba Eduardo, Acta Tropica 97, 1-4 (2006).

Conventional permethrin formulations are prepared by mixing their cis-trans isomers (Pesticide Manual, 11th Edition, CDSTomfin Ed. British Crop Protection Council, UK 1997) and the emulsifiable concentrates of this pyrethroid have also been shown to be effective in treatments. in space treatments on Diptera in general and mosquitoes in particular (Pest and Vector Control, H. van Emden and M. Service, Cambridge University Press, Cambridge, UK, 2004).

A number of compounds have in common their mode of action, which is to interfere with the processes involved in insect growth, hence they are called insect growth regulators, known by their acronym IGR (Insect Growth Regulators). IGR, because of their selectivity and very low mammalian toxicity, is a group of compounds that are widely used today in general insect control (Chemical Pesticides, Mode of Action and Toxicology, J. Stenersen, CRC Press, Boca Raton, USA, 2004)) and of mosquito larvae in particular (Guidelines for Dengue Surveillance and Mosquito Control, World Health Organization, Regional Office for the Western Pacific, Second Edition, Manila, Philippines (2003)).

There are two major types of IGRs, chitin synthesis inhibitors and juveniles, which act as juvenile hormone analogs.

Juvenile IGRs act by interfering with insect maturity in their adult form, as they are mimics of juvenile hormones. (CHEMIC Pesticides, Mode of Action and Toxicology, J. Stenersen, CRC Press, Boca Raton, USA, 2004). This type of compound has been recognized as having the largest larvicidal effect in drinking water treatments for mosquito control (Sixth Edition, WHO / CDS / NTD / Pesticides and their application for control of Public health importance). WHOPES / GCDPP / 2006.1, World Health Organization, 2006). One of these iuvenoidal compounds — 2- (T-methyl-2-4-phenoxyphenoxy> ethoxyUDIDINE) called pyriproxyfen or pyriproxyfen has recently been recommended by the World Health Organization to be used for the control of mosquito larvae in human drinking water. high larvicidal efficacy and safety of use (Pyriproxyfen in drinking water, WHO / SDE / WSH / 07.01 / 10, World Health Organization, 2007).

Insecticide emulsifiable concentrate (EC) or ultra low volume (ULV) formulations, applied spatially as term mists or cold mists, are the most commonly used forms for the control of adult pest insects with larval immature forms and adult flying forms. live in distinct habitat such as mosquitoes (Chavasse DC and Yap HH, Chemical Methods for the Control of Vectors and Pests of Public Health Importance. WHO / CDT / WHOPES / 97.2 pp27, Geneva, Switzerland, 1997). One of the usual criticisms of this type of treatment is its insufficient efficacy on the larvae. (Masuh H., Coto H., Licastro S. and Zerba E. “Control of Aedes aegypti (L.) in Clorinda: A Model for Urban Areas.” Entomol. Vect. 10 (4) 485-494 (2003)) . Likewise, another limitation of this type of space treatment formulations is that when they require diluent aggregate, for maximum effectiveness they must be diluted with organic solvents, particularly de-oil and not water. This requirement responds to the fact that when water is used as a vehicle in place of organic solvents, the effectiveness of insecticide control over pest insects is reduced (Toxicology of Insecticides, F. Matsumura, Plenum Press, London). , 1975). There is a continuing need to prepare novel emulsifiable concentrate-type compositions principally applicable as ultra low volume or thermal mist for the control of insect pests in general and dipterans such as mosquitoes in particular which are ecological. For these asosicons to have a low environmental impact, water should be used as a vehicle without diminishing their adulticidal and larvicidal efficacy by "solvent effect" or by decomposition of assets when applied as thermal mist in aqueous emulsion.

SUMMARY OF THE INVENTION An object of the present invention is a chemically stable, chemically stable, insecticidal and larvicidal emulsifiable concentrate composition for water use as a vehicle for insect pest control which contains as active ingredients a combination of at least one pyrethroid insecticide and juvenile larvicide pyriproxyfen, and whose spatial application in aqueous emulsion has similar or greater efficacy than that obtained with organic diluents.

The formulations of the invention combine a pyrethroid of excellent adulticidal action and pyriproxyfen, a juvenile with a large larvicidal effect, in compositions chemically compatible with the use of water as a carrier while still maintaining their effectiveness in aqueous mist applications. In this way, spatial treatments can be performed using water as a vehicle while still in thermal fog controlling adult insect populations and eliminating living larvae reservoirs with the same or greater effectiveness compared to the use of an organic solvent as medium. for the application. The invention preferably relates to an insecticidal and larvicidal emulsifiable concentrate composition for spatial use in aqueous pest for insect control comprising one or more pyrethroid compounds selected from cis-permethrin, permethrin, cypermethrin, phenothrin, alethrin, resmethrin , cyhalothrin, cyphenothrin, cyfluthrin, deltamethrin, bifenthrin, etofenprox, isomers and mixtures thereof. The present invention is also directed to a ready-made insecticidal and larvicidal composition. Use which comprises an emulsion of an emulsifiable concentrate in a carrier solvent, wherein the emulsifiable concentrate comprises at least one pyrethroid insecticide and the juvenile larvalicide pyriproxifene. Preferably, the carrier solvent is selected from water and organic hydrocarbons such as deodorized kerosene, diesel oil and the like. Even more preferably, the solvent is water.

Also another object of the invention is a method of controlling dengue, yellow fever and malaria mosquito vectors comprising spreading by spraying onto the controllable area of a composition comprising a water emulsion of the concentrate of the invention. to provide a concentration of between 1 and 10 g permethrin containing not less than 80% cis isomer, and between 0.2 and 3 g pyriproxyfen per hectare of surface, most preferably between 1 and 15 g permethrin and between 0.2 and 3 g pyriproxyfen per hectare of surface.

Surprisingly, it has been found that certain combinations of pyrethroid insecticides such as cis-permethrin or permethrin and the insect growth regulator pyriproxyfen can be formulated as stable products, chemically and biologically compatible with the use of water as a vehicle without effectiveness against the alternative use of organic solvents and suitable for that, through spatial applications, even as thermal mists in aqueous environment, cause a great adulticidal and larvicidal efficacy on populations of pest insects in general and dipterous as mosquitoes in particular.

Even more preferably, the invention relates to an insecticidal and larvicidal emulsifiable concentrated composition for spatial use in aqueous medium for the control of pests which comprises as an active ingredient a cis-oermethrin-selected pyrirhoid. prerethrin, cypermethrin, cyhalothrin, deltamethrin, isomers and mixtures thereof; same. The pyrethroid content of the composition is from about 50% (w / v) - preferably from 5 to 30% (w / v). The pyriproxyfen content in the composition is from 0.5 to 20% (w / v), preferably from 2 to 10% (w / v). The emulsifiable concentrate for space use in aqueous medium further comprises at least one surfactant, anti-evaporant, anti-UV protector, antioxidant or defoamer-type excipient dissolved in a suitable solvent.

Among the surfactants that may be employed are, for example, salts of alkylarylsulfonate, ethoxylated polyaryl phenols, ethoxylated castor oil, ethoxylated nonylphenols, mixtures thereof and the like. Preferably, linear or branched calcium (C10 -C13) alkylbenzenesulfonates may be used as salts of alkylarylsulfonate. Preferably, as ethoxylated polyaryl phenols, ethoxylated trystyrylphenol may be used. Preferably, as ethoxylated castor oil, ethoxylated castor oils with 36 to 40 moles of ethoxyl may be used. Preferably, as ethoxylated nonylphenols, ethoxylated nonylphenols with 9 to 14 moles of ethoxyl may be used.

Preferably, surfactants are found in the concentrate of the invention in concentrations ranging from 0.5 to 16% (w / v).

Antievaporants that may be employed include, for example, vegetable oils or esters of their fatty acids or mixtures thereof or polyethylene glycols and the like. Preferably, as vegetable oils corn or soybean oil or mixtures thereof may be used. Preferably, as vegetable fatty acid esters, methyl esters of corn or soybean oil fatty acids or mixtures thereof may be used. Preferably, as polyethylene glycol polyethylene glycol 300 to 600 and 1000 is employed.

Preferably, the anti-evaporants are found in the concentrate of the invention in concentrations ranging from 2 to 50% (w / v).

Among the UV protectors what? may be employed in the concentrate of the invention are found, for example, benzophenone, its derivatives or mixtures thereof and the like.

Preferably, the UV protector employed is benzophenone.

Preferably, UV protectors are found in the concentrate of the invention at concentrations ranging from 0.1 to 5% (w / v).

Among the antioxidants that may be employed in the concentrate of the invention are, for example, alkyl gallates, butyl hydroxy toluene, butyl hydroxy anisole, nordihydroguaiaretic acid, tocopherols, thiodipropionic acid or mixtures thereof and the like.

Preferably, the antioxidants are found in the concentrate of the invention in concentrations ranging from 0.1 to 4% (w / v).

Defoamers which may be employed in the concentrate of the invention are, for example, of the type of silicones, fatty alcohols or mixtures thereof, such as synthetic silicones, octanol, nonanol and mixtures thereof, and the like.

Preferably, the defoamers are found in the concentrate of the invention in concentrations ranging from 0.01 to 1% (w / v).

The solvents that may be employed in the concentrate of the invention are. Pain, for example. of oranoic TiDO as aromatic hydrocarbons such as toluene, xylene etc., ketones such as cyclohexanone, N-methyl-2-pyrrolidone, 2-pyrrolidone, etc. or mixtures thereof and the like. Solvents in the concentrate of the invention are employed to complete the 100% formulation.

Another object of the present invention is a procedure for preparing the emulsifiable concentrate described above. The procedure for preparing an emulsifiable pyrethroid insecticide and juvenile larvicide concentrate of the invention comprises adding a pyrethroid insecticide and a juvenile larvicide, and at least one excipient selected from a surfactant, an anti-evaporant, an anti-UV protector, an antioxidant and a defoamer. in a suitable solvent, and stir until dissolved. The procedure may be performed by the addition of a determined volume of suitable solvent, from 1.25 to 55% of a pyrethroid insecticide and from 1 to 25% of a juvenile larvicide. One continuation incorporates at least one excipient selected from a surfactant, an anti-evaporant, an anti-UV protector, an antioxidant and a defoamer.

Among the surfactants which may be used in the procedure of the invention are mentioned, by way of example, those of the alkylaryl sulfonate salts type, ethoxylated polyaryl phenols, ethoxylated castor oil, ethoxylated nonylphenols or mixtures thereof and the like. Preferably, the surfactants are added to the starting mixture at concentrations ranging from 0.5 to 16% (w / v).

Among the anti-evaporants which may be employed in the procedure of the invention are mentioned, by way of example, those of the type of vegetable oils, esters of their fatty acids or mixtures thereof or polyethylene glycols and the like. Antievaporants are added to the composition of the invention at concentrations ranging from 2 to 50% (w / v).

Among the UV protectors that may be employed in the procedure of the invention is mentioned by way of example benzophenone, its derivatives or mixtures thereof and the like. Preferably, the UV protectors are added to the composition of the invention at concentrations ranging from 0.1 to 5% (w / v).

Among the antioxidants which may be employed in the procedure of the invention are mentioned by way of example alkyl gallates, butyl hydroxy toluene, butyl hydroxy anisole, nordihydroguaiaretic acid, tocopherols, thiodipropionic acid or mixtures thereof and the like. Preferably, the antioxidants are added to the composition of the invention at concentrations ranging from 0.1 to 4% (w / v).

The defoamers used in the procedure of the invention may be, for example, of the type of silicones, fatty alcohols or the like and mixtures thereof. Preferably, the defoamers are aggregated at concentrations ranging from 0.01 to 1.0% (w / v).

Solvents which may be employed in the procedure of the invention are of the organic type as aromatic hydrocarbons such as toluene, xylene etc., ketones such as cyclohexanone, N-methyl-2-pyrrolidone, 2-pyrrolidone, etc., and the like, or the like. mixtures in sufficient quantity to adjust the final content of the active compounds as mentioned above.

For its application, the concentrate of the invention is preferably emulsified in a liquid carrier in a concentrate to carrier volume ratio of from approximately 1: 1000 to approximately 1: 2. Preferably, the concentrate: carrier volume ratio is from about 1: 500 to about 1: 3, more preferably from about 1: 300 to about 1: 4, even more preferably from about 1: 100 to about 1: 5. . The liquid carrier is selected from water and organic solvents such as désodorized quérdsène, diesel oil, biodiesel, etc. Preferably, the liquid carrier is water. The composition obtained from the concentrate of the invention is especially effective for combating pest insects in general, and for spatial control of diptera such as flies and mosquitoes in particular.

The emulsifiable formulations of the invention are particularly suitable for use as an emulsion in water for ultra low volume (cold mist) or thermal mist (smoke) space treatments without the solvent effect or thermal decomposition losing effectiveness with solvent dilutions. in the control of dipterous insects in general and dengue vector mosquitoes (Aedes aegypti) in particular. Suitable doses for environmental treatments of 1 to 25 g of total active compounds per hectare are considered. The composition obtained from the concentrate of the present invention is particularly suitable for application by spraying devices such as thermo-vaporizers, cold mist generators, high-pressure stationary sprayers with motor pumps, mobile pump backpack sprayers, plunger or diaphragm pump, stirrup pump open container sprayers, hand-held intermittent or compression sprayers, trigger sprayers, and other devices generally known in the art. The emulsifiable concentrate of pyrethroid insecticide and juvenile larvicide pyriproxifene based on readily accessible components is formulated through its ability to be applied as cold mist or thermal mist in aqueous medium with the same or greater efficacy than the organic solvent on adult larvae and insects. , improves and simplifies strategies for controlling insect populations in general and dipterans such as mosquitoes in particular with lower environmental impact and greater safety of use. As a general description of the invention, it will be better understood in relation to the following specific examples, which include only the effect of illustrating the invention and not being construed as limiting thereof.

Examples Example 1 Permethrin 10% (w / v) (98% cis isomer), 2% pyriproxyfen, 10% (w / v) of a mixture of calcium dodecyl benzenesulfonate and ethoxylated triestyrylphenol, 10% (wt) are added. / v) soybean oil and 0.1% (w / v) synthetic silicone to an amount of xylene sufficient to achieve 100%. The components were stirred vigorously until dissolved.

The concentration of permethrin (cis 98%) in the formulated prepared by HPLC was verified in a 25 cm long 4.6 cm diameter RP-18 column with 75:25 acetonitrile water at a constant flow of 1, 5 ml / min using ultraviolet detector at 234 nm. To confirm the concentration of pyriproxyfen in the prepared formulation a 25 cm column, MICROSORV PHENYL and acetonitrile / water were used as the eluting solvent in a 65:35 ratio; flow rate 1 ml / min. Detection was performed by ultraviolet detector at 234 nm.

The larvicidal and adulticidal activity on Aedes aegypti of the formulation described in a laboratory scale assay in a 0.35 m3 glass cubic chamber was evaluated. For this purpose an environmental spray (0.85 ml of formulated in 50 ml of water) of the formulated through a hand-capped hole in the front face of the chamber was sprayed by hand spray to achieve a concentration 5 mg / m3 cis-permethrin and 1 mg / m3 pyriproxyfen in aqueous emulsion within the chamber. Inside, 4 disposable containers were placed at the lower angles containing 250 ml of water with 20 Aedes aegypti larvae III. Similarly, 3 wire mesh cages were placed with 10 adult mosquitoes of the same species, each suspended from the upper chamber.

The aqueous dilution of the described formulation was sprayed and the chamber was tightly closed for 1 h. The chamber was opened, ventilated and the adult cages and the larvae pots were removed. Adult mortality in the cages at 2 hours of removal from the cage and non-emergence of adults shortly after the emergence of all adults in the control (13 to 15 days) were evaluated as a parameter indicating the larvicidal effect by interruption of larval development.

The results obtained were 100% adult mortality and 0% adult incubation, which indicates the total mortality of exposed larvae. A test control was conducted with a formulation without active compounds, and the emergence of adults was 100%.

Example 2 10% (w / v) permethrin, 2% pyriproxyfen, 10% (w / v) of a mixture of calcium dodecyl benzene sulfonate and ethoxylated triestyrylphenol, 10% (w / v) soybean oil and 0.1% (w / v) synthetic silicone to an amount of xylene sufficient to 100%. The components were stirred vigorously until dissolved. Asset content was analyzed as described in example 1.

With this composition pre-field tests were carried out by spray-spraying an aqueous dilution of the described formulation of permethrin and pyriproxyfen in a 420 m3 shed performing the application from one end of the shed. used - a dilution of 17.5 ml of formulated in 5 liters of water. Additionally, two similar assays were performed using dilutions of the emulsifiable formulation of permethrin and pyriproxyfen prepared as described in two organic solvents: diesel oil and biodiese. for comparative purposes. In each experiment 330 ml of the dilution (sprayed for 1 minute) were distributed in a house.

Adult mosquitoes (Aedes aegypti) were confined in net-covered disposable plastic cages and III larvae in disposable plastic containers containing 250 ml of water. Mosquito containers were placed on shelves at a height of 1.5 m from the floor and at 3, 6 and 9 m from the spraying machine.

A portable Motan brand machine was used to spray ULV droplet size into the shed described above. Adult mortality was counted shortly after 24 hours of treatment. Larval growth and mortality were followed until full (100%) adult incubation in controls, and at this time, non-emergence of adults in the treated recipients was determined as a parameter indicating the larvicidal effect by stopping larval development.

The results obtained from effect on adult mosquitoes and larvae are summarized in table 1.

Table 1: Mortality of adult mosquitoes and larvae at distinct FORMULA APPLICATION DISTANCES * Measured by non-incubation of adults. Adult incubation in controls was 100%. The results of the pre-field experiment summarized in Table 1 demonstrate that the application of the permethrin + pyriproxyfen emulsifiable concentrate formulation of the present invention, when spatially applied as an aqueous emulsion, has an adulticidal effect on Aedes aegypti mosquitoes similar to that obtained. same formulation formulated in organic solvents. On the other hand, surprisingly, the application of a formulation of the invention to an aqueous emulsion demonstrated a greater larvicidal effect than that obtained from the formulation formulated in organic solvents.

Example 3 15% (w / v) permethrin, 3% pyriproxyfen, 12% (w / v) of a mixture of ethoxylated calcium dodecyl benzene sulfonate, 10% sunflower oil and 0.1% were added. (w / v) synthetic silicone to a sufficient amount of xylene to 100%. The components were stirred vigorously until dissolved. The concentration of permethrin and pyriproxyfen in the emulsifiable formulation was confirmed by HPLC as described in example 1. In the case of permethrin as indicated for c / s-permethrin. The adulticidal and larvicidal effect of the described formulation was measured under laboratory conditions in the 0.35 m3 chamber with final active concentrations of 7.5 mg / m3 permethrin and 1.5 mg / m3 pyriproxyfen within the chamber and with the methodology described in example 1.

The results obtained were 100 *% adult mortality and 0% adult incubation (in the adult emergency control trial it was .100%).

Example 4 A comparative field trial was conducted at the locations of Puerto Libertad and Wanda located in the province of Misiones, Argentina (25 ° 57'51.85 ”S and 54 ° 35'22.90” W).

To perform the test an emulsifiable concentrate formulation of 10% permethrin and 2% pyriproxyfen prepared according to Example 1 was used. Separate dilutions in water and diesel oil were tested separately in various treatment schemes. Four completely separate zones were determined, each with approximately 200 houses. In each of these zones the following treatments were performed: Zone 1: Application of hot mist using solvent water.

Zone 2: Hot mist application using diesel oil as solvent.

Zone 3: Application of cold mist using water as solvent.

Zone 4: Control Zone, untreated. Evaluation of insecticide efficacy was performed on larvae and adults of Aedes aegypti (Diptera: Culicidae).

To evaluate the adulticidal effect, 10 Aedes aegypti adults from 36 to 48 hours of life were transferred to the laboratory. The bioassay cages were transported to the field one hour before treatment and were located between three and five meters. of the line of application. 10 cages were placed per zone.

To evaluate larvicidal activity, 10 250 ml opaque white plastic containers with portable water per zone were placed. They were placed between three and five meters from the application line and within the houses. Fifteen Aedes aegypti larvae III / IV were placed by recipieritè. Ultra low volume (ULV) formulations were applied with a heavy cold mist generating machine ^ P ^ -ULV, Maxi-Pro ™ (Curtis Dyna-Fog. Ltd. Westfieid. Indiana: USA) and heavy mist generating machine hot SWingfog SN 101 (Motan Swingtec GMBH, Isny, Germany) each mounted on the back of a pickup truck.

Three insecticidal compositions were prepared for application in each zone, in addition to the control composition (no active compounds). - Composition for cold mist: 6.6 liters of insecticide formulated, 1.25 liters of polyethylene glycol 1000 and 17.15 liters of water. - Composition for hot mist: 5.2 liters of insecticide formulated, 1 liter of polyethylene glycol 1000 and 14 liters of water. - Composition for hot mist: 5.2 liters of insecticide formulated, 1 liter of polyethylene glycol 1000 and 14 liters of diesel oil.

Through the cold fog machine 379 cm3 of insecticide mixture was released every 36 seconds at a vehicle speed of 10 km per hour. The hot fog machine released 390 cm3 of insecticide mixture every 36 seconds at a vehicle speed of 10 km per hour.

To evaluate the effectiveness of each type of application, a single application dose of 10 grams permethrin / hectare and 2 grams pyriproxyfen / hectare was used.

Approximately one hour after treatment was completed, adult cages and 250 ml containers were taken to the laboratory to determine mortality, which was performed shortly after 24 hours of treatment completion, and emergency adult inhibition on the larvae exposed to spraying.

The following results were obtained: Table 2: Comparative Mortality Values of Adult Mosquitoes and LARVES WITH DISTINCT TREATMENTS * Measured by no adult incubation. Adult Incubation in 100% Controls The results of the field experiment summarized in table 2 demonstrate that the application of the permethrin + pyriproxyfen emulsifiable concentrate formulation of the present invention when applied spatially as an aqueous emulsion has an adulticidal and larvicidal effect on mosquitoes of the species. Aedes aegypti superior to what the same formulated in an organic solvent demonstrates.

Claims (29)

1. Insecticide and larval emulsifiable concentrate for water-treated space-treated insect control comprising a combination of one or more pyrethroid insecticidal compounds and the appropriate juvenile insect hormone analogue piriproxifen and excipients selected from minus a surfactant, an anti-evaporant, an anti-UV protector, an antioxidant, a defoamer and a suitable organic solvent. Insecticide and larval emulsifiable concentrate according to claim 1, characterized in that said insecticides, pyrethroids are selected from: c / s-permethrin, permethrin, pretrethin, phenothrin, alethrin, resmetrin, cyhalothrin, eifenotrin , cyfluthrin, deltamethrin, bifentrin, etofenprox, their isomers and combinations thereof. The insecticidal and larval emulsifiable concentrate according to claim 1, characterized in that the pyrethroid insecticide is c / s-permethrin, permethrin, cypermethrin, cyhalothrin, deltamethrin, or isomers thereof or a combination thereof. The emulsifiable and insoluble concentrate according to claim 1, characterized in that the pyrethroid insecticide is permethrin or its cis isomer. Insecticide and larval emulsifiable concentrate according to any one of the preceding claims, characterized in that the pyrethroid insecticide content is between 1 and 50% w / v. Insecticide and larval emulsifiable concentrate according to Claim 5, characterized in that the pyrethroid insecticide content is between 5 and 30% w / v. Insecticide and larval emulsifier concentrate according to claim 6, characterized in that the pyrethroid insecticide content is 10% w / v. Insecticide and larval emulsifier concentrate according to Claim 1, characterized in that the juvenile larval killer pyriproxyfen is present in a ratio of 0.5 to 20% w / v. The insecticidal and larval emulsifiable concentrate according to claim 8, characterized in that the juvenile piriproxifen larvicide is present in a proportion of from 2 to 10% w / v. Insecticide-emulsifiable concentrate according to claim 1, characterized in that the pyrethroid is c / s-permethrin. The emulsifiable and insoluble concentrate according to claim 1, characterized in that the pyrethroid is permethrin. An emulsifiable and leached emulsifier according to claim 1, characterized in that it contains a surfactant selected from salts of alkylarylsulfonate, ethoxylated polyaryl phenols, ethoxylated castor oil, ethoxylated nonylphenols and combinations thereof. An emulsifiable and leached emulsifier according to claim 12, characterized in that the surfactant is selected from linear or branched calcium (C10-C13) -benzenesulfonate, ethoxylated castor oil, 36-ethoxylated castor oil. to 40 moles of ethoxyl, ethoxylated nonylphenol with 9 to 14 moles of ethoxyl and combinations thereof. Insecticide and larval emulsifier concentrate according to Claim 1, characterized in that it contains between 2 and 50.0% (w / v) of an antievaporant selected from vegetable oils or esters of their fatty acids or mixtures thereof. or polyethylethylene glycols, and combinations thereof. Insecticide and larval emulsifier concentrate according to claim 1, characterized in that it contains between 0.1 and 5% (w / v) of an anti-UV protector selected from benzophenone, its derivatives and combinations thereof. An emulsifiable and leaching emulsifier according to claim 1, characterized in that it contains from 0.1 to 4% (w / v) of an antioxidant selected from alkyl gallates, butyl hydroxy toluene, butyl hydroxy anisole, nordihydroguaiaretic acid, tocopherols, thiodipropionic acid and combinations thereof. 17. The emulsifiable and insoluble emulsion according to claim 1, characterized in that it contains between 0.01 and 1% (w / v) of a defoamer selected from silicones, fatty alcohols and combinations thereof. 4 An emulsifiable and leached emulsifier according to claim 1, characterized in that the solvent is selected from aromatic hydrocarbons, ketones and combinations thereof. The emulsifiable and insoluble emulsified concentrate according to claim 18, characterized in that the solvent is selected from toluene, xylene, cyclohexanone, N-methyl-2-pyrrolidone and combinations thereof. Method for the preparation of an insecticeable and larval emulsifiable concentrate according to claim 1, characterized in that it comprises permethrin, pyriproxyfen and at least one excipient selected from a surfactant, an antievaporant, an anti-UV protector; an antioxidant and a defoamer in a sufficient amount of suitable organic solvent and dissolve upon stirring. A ready-to-use insecticide and larval composition comprising an emulsifiable emulsion concentrate according to any one of claims 1 to 19, in a liquid carrier, wherein the concentrate: carrier ratio is approximately 1: 1000 to approximately 1: 2. READY FOR USE INSECTICIDE AND LARVICIDE COMPOSITION according to claim 21, characterized in that the concentrate: carrier ratio is from approximately 1: 300 to approximately 1: 4. READY-USE INSECTICIDE AND LARVICIDE COMPOSITION according to claim 21, characterized in that the ratio of concentrate: carrier is from approximately 1: 100 to approximately 1: 5. ? INSECTICIDE AND LARVICIDE COMPOSITION READY FOR USE according to any one of claims 21 to 23, characterized in that the carrier is selected from water and a solvent. INSECTICIDE AND LARVICIDE COMPOSITION READY FOR USE according to any one of claims 21 to 23, characterized in that the carrier is water. Use of an insecticide and larval composition according to claim 21 for the control of mosquitoes. Use of an insecticide and larval composition according to claim 26 for the control of the Aedes aegypti mosquito vector of dengue fever and yellow fever. Method of controlling mosquitoes, dengue vectors, yellow fever and malaria, characterized in that a composition according to any one of claims 21 to 25 is spread by spraying onto the susceptible area to control. provide a concentration of between 1 and 10 g permethrin containing at least 80% cis isomer, and between 0.2 and 3 g pyriproxyfen per hectare of surface. Method of controlling mosquitoes, dengue vectors, yellow fever and malaria, characterized in that a composition according to any one of claims 21 to 25 is spread by spraying onto the susceptible area to provide control. a concentration between 1 and 15 g permethrin and between 0.2 and 3 g pyriproxyfen per hectare of surface.