BRPI0516329A - multipurpose RNA molecules comprising at least one aptamer for the application of double-stranded RNA to pest organisms - Google Patents

multipurpose RNA molecules comprising at least one aptamer for the application of double-stranded RNA to pest organisms

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Publication number
BRPI0516329A
BRPI0516329A BRPI0516329-3A BRPI0516329A BRPI0516329A BR PI0516329 A BRPI0516329 A BR PI0516329A BR PI0516329 A BRPI0516329 A BR PI0516329A BR PI0516329 A BRPI0516329 A BR PI0516329A
Authority
BR
Brazil
Prior art keywords
rna
application
nucleotide sequence
double
aptamer
Prior art date
Application number
BRPI0516329-3A
Other languages
Portuguese (pt)
Inventor
Thierry Andre Olivier Bogaert
Pascale Feldmann
Geert Plaetinck
Original Assignee
Devgen Nv
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Filing date
Publication date
Application filed by Devgen Nv filed Critical Devgen Nv
Publication of BRPI0516329A publication Critical patent/BRPI0516329A/en

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8285—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for nematode resistance
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3519—Fusion with another nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2320/00—Applications; Uses
    • C12N2320/30—Special therapeutic applications
    • C12N2320/32—Special delivery means, e.g. tissue-specific
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Abstract

MOLéCULAS DE RNA DE MULTIDOMìNIO QUE COMPREENDEM PELO MENOS UM APTáMERO PARA A APLICAçãO DE RNA DE CORDãO DUPLO A ORGANISMOS DE PESTES, particularmente a A presente invenção refere-se a métodos e a construtos para a aplicação do RNA de cordão duplo (dsRNA) aos organismos de peste. Mais especificamente, a invenção refere-se a uma molécula de RNA de multidomínio que consiste em uma seqüência de nucleotídeo que compreende (i) pelo menos um aptâmero e (ii) pelo menos uma seqüência de nucleotídeo de interesse que forma o RNA de cordão duplo, sendo que o dito RNA de cordão duplo compreende os cordões complementares reanelados, em que cada um deles compreende uma seqüência de nucleotídeo que é complementar a pelo menos parte de uma seqüência de nucleotídeo alvo da peste. A invenção refere-se adicionalmente aos ácidos nucléicos que codificam as moléculas de RNA de multidomínio e a vários usos das moléculas de RNA de multidomínio na agricultura.MULTIDOMAIN RNA MOLECULES UNDERSTANDING AT LEAST ONE APPLICATION FOR DOUBLE CORD RNA APPLICATION TO PEST ORGANISMS, particularly The present invention relates to methods and constructs for the application of double stranded RNA (dsRNA) to organisms. of plague. More specifically, the invention relates to a multi-domain RNA molecule consisting of a nucleotide sequence comprising (i) at least one aptamer and (ii) at least one nucleotide sequence of interest forming double stranded RNA. wherein said double stranded RNA comprises the complementary stranded strands, each of which comprises a nucleotide sequence that is complementary to at least part of a plague target nucleotide sequence. The invention further relates to nucleic acids encoding multi-domain RNA molecules and various uses of multi-domain RNA molecules in agriculture.

BRPI0516329-3A 2004-10-25 2005-10-25 multipurpose RNA molecules comprising at least one aptamer for the application of double-stranded RNA to pest organisms BRPI0516329A (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US62180104P 2004-10-25 2004-10-25
EP04447235 2004-10-25
US62902704P 2004-11-18 2004-11-18
EP04447251 2004-11-18
PCT/EP2005/011439 WO2006045590A2 (en) 2004-10-25 2005-10-25 Multidomain rna molecules comprising at least one aptamer for delivering double stranded rna to pest organisms

Publications (1)

Publication Number Publication Date
BRPI0516329A true BRPI0516329A (en) 2008-03-11

Family

ID=56290744

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0516329-3A BRPI0516329A (en) 2004-10-25 2005-10-25 multipurpose RNA molecules comprising at least one aptamer for the application of double-stranded RNA to pest organisms

Country Status (6)

Country Link
EP (1) EP1805308A2 (en)
AU (1) AU2005298827B2 (en)
BR (1) BRPI0516329A (en)
CA (1) CA2583334A1 (en)
MX (1) MX2007004686A (en)
WO (1) WO2006045590A2 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7612194B2 (en) 2001-07-24 2009-11-03 Monsanto Technology Llc Nucleic acid sequences from Diabrotica virgifera virgifera LeConte and uses thereof
US20050256071A1 (en) * 2003-07-15 2005-11-17 California Institute Of Technology Inhibitor nucleic acids
US20060021087A1 (en) 2004-04-09 2006-01-26 Baum James A Compositions and methods for control of insect infestations in plants
CA2622671C (en) * 2005-09-16 2020-12-22 Devgen Nv Methods for controlling pests using rnai
PL2431473T3 (en) 2005-09-16 2017-05-31 Monsanto Technology Llc Methods for genetic control of insect infestations in plants and compositions thereof
JP2009538626A (en) * 2006-06-01 2009-11-12 デューク ユニバーシティ Delivery method
US8809625B2 (en) 2008-01-17 2014-08-19 Pioneer Hi-Bred International, Inc. Compositions and methods for the suppression of target polynucleotides from Lygus
US8367895B2 (en) 2008-01-17 2013-02-05 Pioneer Hi-Bred International, Inc. Compositions and methods for the suppression of target polynucleotides from the family aphididae
US8847013B2 (en) 2008-01-17 2014-09-30 Pioneer Hi Bred International Inc Compositions and methods for the suppression of target polynucleotides from lepidoptera
WO2011153418A2 (en) 2010-06-04 2011-12-08 Broglie Karen E Compositions and methods for insecticidal control of stinkbugs
ES2751497T3 (en) 2013-12-20 2020-03-31 Univ Queensland Plant protection iRNA compositions comprising plant protection double-stranded RNA adsorbed on double layered hydroxide particles
WO2016201523A1 (en) 2015-06-19 2016-12-22 The University Of Queensland Composition
CN112899301B (en) * 2021-01-29 2023-04-11 中国热带农业科学院热带生物技术研究所 Cassava common mosaic virus induced gene silencing system and application thereof

Also Published As

Publication number Publication date
AU2005298827A1 (en) 2006-05-04
EP1805308A2 (en) 2007-07-11
WO2006045590A3 (en) 2006-06-15
AU2005298827B2 (en) 2011-09-08
MX2007004686A (en) 2007-06-14
WO2006045590A2 (en) 2006-05-04
CA2583334A1 (en) 2006-05-04

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