BRPI0108436B8 - method for producing primate embryo bodies from primate embryo stem cell colonies that are adherent to a substrate - Google Patents

method for producing primate embryo bodies from primate embryo stem cell colonies that are adherent to a substrate

Info

Publication number
BRPI0108436B8
BRPI0108436B8 BRPI0108436A BRPI0108436B8 BR PI0108436 B8 BRPI0108436 B8 BR PI0108436B8 BR PI0108436 A BRPI0108436 A BR PI0108436A BR PI0108436 B8 BRPI0108436 B8 BR PI0108436B8
Authority
BR
Brazil
Prior art keywords
primate
primate embryo
bodies
adherent
cells
Prior art date
Application number
Other languages
Portuguese (pt)
Inventor
A Thomson James
J Swiergiel Jennifer
S Marshall Vivienne
Original Assignee
Wisconsin Alumni Res Found
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wisconsin Alumni Res Found filed Critical Wisconsin Alumni Res Found
Publication of BR0108436A publication Critical patent/BR0108436A/en
Publication of BRPI0108436B1 publication Critical patent/BRPI0108436B1/en
Publication of BRPI0108436B8 publication Critical patent/BRPI0108436B8/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

método para a produção de corpos embrióides a partir de células tronco embriônicas de primatas. corpos embriônicos primatas são formados a partir de células es primatas. as células es formam agrupamentos. em seguida se remove os agrupamentos como grupos, e permite a incubação sob condições não-aderentes. o desenvolvimento dos corpos embrióides a partir de células es primatas é dependente da manutenção da agregação das células, na medida em que as células individualizadas irão rapidamente morrer.method for producing embryoid bodies from primate embryonic stem cells. Primate embryonic bodies are formed from primate cells. cells and form clusters. then remove the clusters as groups, and allow incubation under non-adherent conditions. the development of embryoid bodies from primate cells is dependent on maintaining cell aggregation, as individualized cells will rapidly die.

BRPI0108436-4 2000-02-21 2001-02-20 method for producing primate embryo bodies from primate embryo stem cell colonies that are adherent to a substrate BRPI0108436B8 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/510,444 US6602711B1 (en) 2000-02-21 2000-02-21 Method of making embryoid bodies from primate embryonic stem cells
US09/510,444 2000-02-21
PCT/US2001/005252 WO2001062899A2 (en) 2000-02-21 2001-02-20 Method of making embryoid bodies from primate embryonic stem cells

Publications (3)

Publication Number Publication Date
BR0108436A BR0108436A (en) 2003-03-25
BRPI0108436B1 BRPI0108436B1 (en) 2021-02-23
BRPI0108436B8 true BRPI0108436B8 (en) 2021-05-25

Family

ID=24030756

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0108436-4 BRPI0108436B8 (en) 2000-02-21 2001-02-20 method for producing primate embryo bodies from primate embryo stem cell colonies that are adherent to a substrate

Country Status (15)

Country Link
US (2) US6602711B1 (en)
EP (1) EP1257634A2 (en)
JP (1) JP5339661B2 (en)
KR (1) KR100812856B1 (en)
CN (1) CN1404526A (en)
AU (3) AU3849101A (en)
BR (1) BRPI0108436B8 (en)
CA (1) CA2400158C (en)
HK (1) HK1052951A1 (en)
IL (2) IL151176A0 (en)
IS (1) IS6514A (en)
MX (1) MXPA02008054A (en)
NO (1) NO20023949D0 (en)
NZ (1) NZ520700A (en)
WO (1) WO2001062899A2 (en)

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Also Published As

Publication number Publication date
CA2400158A1 (en) 2001-08-30
WO2001062899A3 (en) 2002-02-21
IL208748A (en) 2015-01-29
HK1052951A1 (en) 2003-10-03
AU3849101A (en) 2001-09-03
JP5339661B2 (en) 2013-11-13
US7220584B2 (en) 2007-05-22
NZ520700A (en) 2004-04-30
WO2001062899A2 (en) 2001-08-30
IL151176A0 (en) 2003-04-10
JP2003523766A (en) 2003-08-12
CN1404526A (en) 2003-03-19
AU2006203588A1 (en) 2006-09-07
IS6514A (en) 2002-08-20
KR20030032921A (en) 2003-04-26
BR0108436A (en) 2003-03-25
US20040023376A1 (en) 2004-02-05
IL208748A0 (en) 2010-12-30
EP1257634A2 (en) 2002-11-20
CA2400158C (en) 2006-04-04
US6602711B1 (en) 2003-08-05
KR100812856B1 (en) 2008-03-11
AU2001238491B2 (en) 2006-05-18
NO20023949L (en) 2002-08-20
NO20023949D0 (en) 2002-08-20
BRPI0108436B1 (en) 2021-02-23
MXPA02008054A (en) 2003-01-28

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