BR9902792A - Escherichia coli host / vector system based on antibiotic-free selection by complementing an auxotrophy - Google Patents
Escherichia coli host / vector system based on antibiotic-free selection by complementing an auxotrophyInfo
- Publication number
- BR9902792A BR9902792A BR9902792-5A BR9902792A BR9902792A BR 9902792 A BR9902792 A BR 9902792A BR 9902792 A BR9902792 A BR 9902792A BR 9902792 A BR9902792 A BR 9902792A
- Authority
- BR
- Brazil
- Prior art keywords
- auxotrophy
- antibiotic
- free selection
- escherichia coli
- vector system
- Prior art date
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Patente de Invenção: <B>"SISTEMA DE HOSPEDEIRO/VETOR DE ESCHERICHIA COLI BASEADO SOBRE SELEçãO ISENTA DE ANTIBIóTICO POR MEIO DE COMPLEMENTAçãO DE UMA AUXOTROFIA"<D>. Vetor procariótico mínimo de expressão, o qual não pode ser homologamente recombinado com o genoma dos organismos procarióticos compreendendo: (e) uma origem de replicação, f) um gene identificador de auxotrofia, g) um promotor, o qual é funcionalmente ativo em procarióticas e h) um gene externo a ser expresso, o qual está sob o controle do referido promotor é particularmente adequado para seleção isenta de antibióticos.Invention Patent: <B> "HOSTING / VECTOR SYSTEM OF ESCHERICHIA COLI BASED ON ANTIBIOTIC-FREE SELECTION THROUGH COMPLEMENTATION OF AN AUXOTROPHY" <D>. Minimum prokaryotic expression vector, which cannot be homologously recombined with the genome of prokaryotic organisms comprising: (e) an origin of replication, f) an auxotrophy identifying gene, g) a promoter, which is functionally active in prokaryotic eh ) an external gene to be expressed, which is under the control of said promoter, is particularly suitable for antibiotic-free selection.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP98113156 | 1998-07-15 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| BR9902792A true BR9902792A (en) | 2000-05-09 |
| BR9902792B1 BR9902792B1 (en) | 2010-11-16 |
Family
ID=8232274
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR9902792A BR9902792B1 (en) | 1998-07-15 | 1999-07-14 | minimal prokaryotic expression vector, prokaryotic host cell, process for constructing a prokaryotic expression system, as well as process for producing a heterologous protein. |
Country Status (4)
| Country | Link |
|---|---|
| AR (1) | AR020111A1 (en) |
| BR (1) | BR9902792B1 (en) |
| TR (1) | TR199901650A2 (en) |
| ZA (1) | ZA994552B (en) |
-
1999
- 1999-07-13 AR ARP990103416 patent/AR020111A1/en active IP Right Grant
- 1999-07-14 BR BR9902792A patent/BR9902792B1/en not_active IP Right Cessation
- 1999-07-14 ZA ZA9904552A patent/ZA994552B/en unknown
- 1999-07-14 TR TR1999/01650A patent/TR199901650A2/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| TR199901650A3 (en) | 2000-02-21 |
| TR199901650A2 (en) | 2000-02-21 |
| AR020111A1 (en) | 2002-04-10 |
| ZA994552B (en) | 2000-01-17 |
| BR9902792B1 (en) | 2010-11-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B07A | Technical examination (opinion): publication of technical examination (opinion) | ||
| B09A | Decision: intention to grant | ||
| B16A | Patent or certificate of addition of invention granted |
Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 16/11/2010, OBSERVADAS AS CONDICOES LEGAIS. |
|
| B21A | Expiry acc. art. 78, item i of ipl- expiry of the term of protection |
Free format text: PATENTE EXTINTA EM 16.11.2020 |