BR9815260A - Binary transgenic viral expression system, method of altering the expression of an endogenous or transgene plant gene in a plant, a method of altering the levels of a protein encoded by a target gene in a plant and a viral replication system. - Google Patents

Binary transgenic viral expression system, method of altering the expression of an endogenous or transgene plant gene in a plant, a method of altering the levels of a protein encoded by a target gene in a plant and a viral replication system.

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Publication number
BR9815260A
BR9815260A BR9815260-2A BR9815260A BR9815260A BR 9815260 A BR9815260 A BR 9815260A BR 9815260 A BR9815260 A BR 9815260A BR 9815260 A BR9815260 A BR 9815260A
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BR
Brazil
Prior art keywords
plant
gene
expression
replication
viral
Prior art date
Application number
BR9815260-2A
Other languages
Portuguese (pt)
Inventor
Narendra S Yadav
Original Assignee
Du Pont
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Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of BR9815260A publication Critical patent/BR9815260A/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8217—Gene switch
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8238—Externally regulated expression systems chemically inducible, e.g. tetracycline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/12011—Geminiviridae
    • C12N2750/12022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

"SISTEMA DE EXPRESSãO VIRAL TRANSGêNICO BINáRIO, MéTODO DE ALTERAçãO DA EXPRESSãO DE GENE VEGETAL ENDóGENO OU TRANSGENE EM UMA PLANTA, MéTODO DE ALTERAçãO DOS NìVEIS DE UMA PROTEìNA CODIFICADA POR UM GENE ALVO EM UMA PLANTA E SISTEMA DE REPLICAçãO VIRAL" A presente invenção refere-se a um sistema de expressão viral em vegetais binária regulada. Ele compreende dois componentes cromossomicamente integrados. Um componente é um proreplicon, o qual contém seq³ências virais de ação cis necessárias para a replicação e contém um gene alvo. O outro componente é um gene de replicação de ação trans que compreende um promotor regulado operacionalmente ligado à região codificadora para uma proteína de replicação viral. O proreplicon não contém o gene de replicação essencial para a replicação de replicon, e dessa maneira não pode se submeter à replicação episomal autónoma. Entretanto, a expressão regulada da proteína de replicação de ação trans nas células de vegetais que também contém o proreplicon vai acionar a liberação do replicon livre do proreplicon integrado, resulta em sua replicação episomal in trans, e resulta na expressão do gene alvo, caso esteja presente, através da amplificação de genes. O sistema de expressão é útil para produção de proteínas estranhas, bem como o silenciamento de genes e transgenes endógenos no tecido vegetal. A expressão específica de tecido é controlada pela escolha do promotor que controla a transcrição do gene de replicação de ação trans."BINARY TRANSGENIC VIRAL EXPRESSION SYSTEM, METHOD OF CHANGING THE EXPRESSION OF ENDOGENOUS VEGETABLE GENE OR TRANSGENE IN A PLANT, METHOD OF CHANGING THE LEVELS OF A PROTEIN ENCODED BY A TARGETED GENE IN A PLANT AND REVIEW OF A PLANT AND REVIEW to a regulated binary vegetable viral expression system. It comprises two chromosomally integrated components. One component is a proreplicon, which contains viral cis-action sequences necessary for replication and contains a target gene. The other component is a trans-acting replication gene that comprises a regulated promoter operably linked to the coding region for a viral replicating protein. Proreplicon does not contain the replication gene essential for replicon replication, and therefore cannot undergo autonomous episomal replication. However, regulated expression of the trans-acting replication protein in plant cells that also contains the proreplicon will trigger the release of the free replicon from the integrated proreplicon, results in its episomal replication in trans, and results in the expression of the target gene, if present. present through gene amplification. The expression system is useful for the production of foreign proteins, as well as the silencing of genes and endogenous transgenes in plant tissue. Tissue-specific expression is controlled by the choice of the promoter that controls the transcription of the trans-acting replication gene.

BR9815260-2A 1997-10-24 1998-10-23 Binary transgenic viral expression system, method of altering the expression of an endogenous or transgene plant gene in a plant, a method of altering the levels of a protein encoded by a target gene in a plant and a viral replication system. BR9815260A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US6350497P 1997-10-24 1997-10-24
US10155898P 1998-09-23 1998-09-23
PCT/US1998/022688 WO1999022003A1 (en) 1997-10-24 1998-10-23 Binary viral expression system in plants

Publications (1)

Publication Number Publication Date
BR9815260A true BR9815260A (en) 2000-11-21

Family

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Family Applications (1)

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BR9815260-2A BR9815260A (en) 1997-10-24 1998-10-23 Binary transgenic viral expression system, method of altering the expression of an endogenous or transgene plant gene in a plant, a method of altering the levels of a protein encoded by a target gene in a plant and a viral replication system.

Country Status (7)

Country Link
EP (1) EP1025234A1 (en)
AU (1) AU731330B2 (en)
BR (1) BR9815260A (en)
CA (1) CA2304984A1 (en)
HU (1) HUP0004222A3 (en)
PL (1) PL340297A1 (en)
WO (1) WO1999022003A1 (en)

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Publication number Priority date Publication date Assignee Title
US6759571B1 (en) 1998-04-01 2004-07-06 North Carolina State University Method of suppressing gene expression in plants
EP1068340B1 (en) * 1998-04-01 2009-09-23 North Carolina State University Method of suppressing gene expression in plants
US7468475B2 (en) 2000-06-16 2008-12-23 Schmuelling Thomas Method for modifying plant morphology, biochemistry and physiology
US6512165B1 (en) 2000-07-10 2003-01-28 Pioneer Hi-Bred International, Inc. Methods for enhancing plant transformation frequencies
MXPA06003715A (en) 2003-11-10 2006-06-23 Icon Genetics Ag Rna virus-derived plant expression system.
ES2346224T4 (en) 2003-11-10 2011-06-01 Icon Genetics Gmbh PLANT EXPRESSION SYSTEM DERIVED FROM ARN VIRUSES.
EP1564295A1 (en) 2004-01-23 2005-08-17 Icon Genetics AG RNA virus-derived plant expression system
EP1616959A1 (en) 2004-07-07 2006-01-18 Icon Genetics AG Biological safe transient protein expression in plants
CA2667396C (en) 2006-09-25 2017-03-28 Freie Universitaet Berlin Transcriptional repressors of cytokinin signaling and their use
ATE550350T1 (en) 2009-07-10 2012-04-15 Univ Berlin Freie ROCK2 AND ROCK3, TWO NEW GAIN-OF-FUNCTION VARIANTS OF THE CYTOKIN RECEPTORS AHK2 AND AHK3
EP2418283A1 (en) 2010-08-07 2012-02-15 Nomad Bioscience GmbH Process of transfecting plants
EP2584042A1 (en) 2011-10-17 2013-04-24 Nomad Bioscience GmbH Production, storage and use of cell wall-degrading enzymes
EP2647715A1 (en) 2012-04-03 2013-10-09 Nomad Bioscience GmbH Agrobacterium for transient transfection of whole plants
AU2014270707B2 (en) 2013-05-23 2019-12-12 Nomad Bioscience Gmbh Process of providing plants with abiotic stress resistance
EP3097783B1 (en) 2015-05-26 2019-11-13 Nomad Bioscience GmbH Colicin m for the control of ehec
EP3372091A1 (en) 2017-03-07 2018-09-12 Nomad Bioscience GmbH Method of reducing contamination of an object with clostridium
EP3378485A1 (en) 2017-03-24 2018-09-26 Nomad Bioscience GmbH Bacteriocins for control of salmonella enterica
JP2021514391A (en) 2018-02-15 2021-06-10 アイコン ジェネティクス ゲーエムベーハー Immunogenic compositions and vaccines to generate an immune response against norovirus
CA3133030A1 (en) 2019-03-12 2020-09-17 Icon Genetics Gmbh Norovirus-like particles with improved stability
JP2022536068A (en) 2019-06-06 2022-08-12 ノマド バイオサイエンス ゲゼルシャフト ミット ベシュレンクテル ハフツング Klevicin for control of Klebsiella
WO2022012926A1 (en) 2020-07-16 2022-01-20 Nomad Bioscience Gmbh Products for oral consumption with reduced sugar content
EP4248987A1 (en) 2022-03-21 2023-09-27 Nomad Bioscience GmbH Chimeric bacteriocins and method for the control of pseudomonas

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0221044B1 (en) * 1985-10-25 1992-09-02 Monsanto Company Novel plant vectors
NL9001711A (en) * 1989-10-03 1991-05-01 Clovis Matton N V GENETIC MANIPULATIONS WITH RECOMBINANT DNA INCLUDING SEQUENCES DERIVED FROM RNA VIRUS.
PT686197E (en) * 1993-02-26 2000-11-30 Calgene Llc GEMINIVIRUS-BASED GENE EXPRESSION SYSTEM
US6077992A (en) * 1997-10-24 2000-06-20 E. I. Du Pont De Nemours And Company Binary viral expression system in plants

Also Published As

Publication number Publication date
PL340297A1 (en) 2001-01-29
AU731330B2 (en) 2001-03-29
EP1025234A1 (en) 2000-08-09
WO1999022003A1 (en) 1999-05-06
CA2304984A1 (en) 1999-05-06
AU1122599A (en) 1999-05-17
HUP0004222A2 (en) 2001-03-28
HUP0004222A3 (en) 2002-11-28

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