BR9510679A - Expansion of bone marrow stromal cells. - Google Patents
Expansion of bone marrow stromal cells.Info
- Publication number
- BR9510679A BR9510679A BR9510679-0A BR9510679A BR9510679A BR 9510679 A BR9510679 A BR 9510679A BR 9510679 A BR9510679 A BR 9510679A BR 9510679 A BR9510679 A BR 9510679A
- Authority
- BR
- Brazil
- Prior art keywords
- stromal cells
- bone marrow
- marrow stromal
- expansion
- culture medium
- Prior art date
Links
Abstract
<B>EXPANSãO DE CéLULAS ESTROMAIS DE MEDULA óSSEA<D>. A invenção apresenta um processo para a seleção e expansão de células estromais de medula óssea. O processo inclui as etapas de obter células estromais de medula óssea, introduzir as células estromais em um vaso pré-revestido em uma superfície interna com uma gelatina, e conter uma meio de cultura incluindo um polipeptídeo de fator de crescimento de fibroblasto acídico ("aFGF"), e expandir as células estromais no meio de cultura sob condições e por um tempo suficiente para obter um número aumentado de células estromais de medula óssea. O meio de cultura pode incluir, adicionalmente, heparina, e o vaso pode ser adicionalmente pré-revestido com soro bovino fetal.<B> BONE MARROW STROMAL CELL EXPANSION <D>. The invention presents a process for the selection and expansion of bone marrow stromal cells. The process includes the steps of obtaining bone marrow stromal cells, introducing the stromal cells into a pre-coated vessel on an inner surface with a gelatin, and containing a culture medium including an acidic fibroblast growth factor polypeptide ("aFGF "), and expand stromal cells in the culture medium under conditions and long enough to obtain an increased number of bone marrow stromal cells. The culture medium can additionally include heparin, and the vessel can be additionally pre-coated with fetal bovine serum.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
BR9510679-0A BR9510679A (en) | 1995-12-29 | 1995-12-29 | Expansion of bone marrow stromal cells. |
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN95198024A CN1209836A (en) | 1995-12-29 | 1995-12-29 | Expansion of bone marrow stromal cells |
BR9510679-0A BR9510679A (en) | 1995-12-29 | 1995-12-29 | Expansion of bone marrow stromal cells. |
PCT/US1995/017073 WO1997024434A1 (en) | 1995-12-29 | 1995-12-29 | Expansion of bone marrow stromal cells |
CA002240790A CA2240790C (en) | 1995-12-29 | 1995-12-29 | Expansion of bone marrow stromal cells |
US08/581,059 US5766950A (en) | 1995-12-29 | 1995-12-29 | Expansion of bone marrow stromal cells |
Publications (1)
Publication Number | Publication Date |
---|---|
BR9510679A true BR9510679A (en) | 1999-11-30 |
Family
ID=27507847
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR9510679-0A BR9510679A (en) | 1995-12-29 | 1995-12-29 | Expansion of bone marrow stromal cells. |
Country Status (1)
Country | Link |
---|---|
BR (1) | BR9510679A (en) |
-
1995
- 1995-12-29 BR BR9510679-0A patent/BR9510679A/en not_active Application Discontinuation
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FB34 | Technical and formal requirements: requirement - article 34 of industrial property law | ||
FA14 | Dismissal: dismissal - article 34 of industrial property law | ||
B11T | Dismissal of application maintained [chapter 11.20 patent gazette] |