BR9510679A - Expansion of bone marrow stromal cells. - Google Patents

Expansion of bone marrow stromal cells.

Info

Publication number
BR9510679A
BR9510679A BR9510679-0A BR9510679A BR9510679A BR 9510679 A BR9510679 A BR 9510679A BR 9510679 A BR9510679 A BR 9510679A BR 9510679 A BR9510679 A BR 9510679A
Authority
BR
Brazil
Prior art keywords
stromal cells
bone marrow
marrow stromal
expansion
culture medium
Prior art date
Application number
BR9510679-0A
Other languages
Portuguese (pt)
Inventor
Joel S Greenberger
David R Hurwitz
Original Assignee
Alg Company
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alg Company filed Critical Alg Company
Priority to BR9510679-0A priority Critical patent/BR9510679A/en
Priority claimed from CN95198024A external-priority patent/CN1209836A/en
Priority claimed from CA002240790A external-priority patent/CA2240790C/en
Publication of BR9510679A publication Critical patent/BR9510679A/en

Links

Abstract

<B>EXPANSãO DE CéLULAS ESTROMAIS DE MEDULA óSSEA<D>. A invenção apresenta um processo para a seleção e expansão de células estromais de medula óssea. O processo inclui as etapas de obter células estromais de medula óssea, introduzir as células estromais em um vaso pré-revestido em uma superfície interna com uma gelatina, e conter uma meio de cultura incluindo um polipeptídeo de fator de crescimento de fibroblasto acídico ("aFGF"), e expandir as células estromais no meio de cultura sob condições e por um tempo suficiente para obter um número aumentado de células estromais de medula óssea. O meio de cultura pode incluir, adicionalmente, heparina, e o vaso pode ser adicionalmente pré-revestido com soro bovino fetal.<B> BONE MARROW STROMAL CELL EXPANSION <D>. The invention presents a process for the selection and expansion of bone marrow stromal cells. The process includes the steps of obtaining bone marrow stromal cells, introducing the stromal cells into a pre-coated vessel on an inner surface with a gelatin, and containing a culture medium including an acidic fibroblast growth factor polypeptide ("aFGF "), and expand stromal cells in the culture medium under conditions and long enough to obtain an increased number of bone marrow stromal cells. The culture medium can additionally include heparin, and the vessel can be additionally pre-coated with fetal bovine serum.

BR9510679-0A 1995-12-29 1995-12-29 Expansion of bone marrow stromal cells. BR9510679A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
BR9510679-0A BR9510679A (en) 1995-12-29 1995-12-29 Expansion of bone marrow stromal cells.

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
CN95198024A CN1209836A (en) 1995-12-29 1995-12-29 Expansion of bone marrow stromal cells
BR9510679-0A BR9510679A (en) 1995-12-29 1995-12-29 Expansion of bone marrow stromal cells.
PCT/US1995/017073 WO1997024434A1 (en) 1995-12-29 1995-12-29 Expansion of bone marrow stromal cells
CA002240790A CA2240790C (en) 1995-12-29 1995-12-29 Expansion of bone marrow stromal cells
US08/581,059 US5766950A (en) 1995-12-29 1995-12-29 Expansion of bone marrow stromal cells

Publications (1)

Publication Number Publication Date
BR9510679A true BR9510679A (en) 1999-11-30

Family

ID=27507847

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9510679-0A BR9510679A (en) 1995-12-29 1995-12-29 Expansion of bone marrow stromal cells.

Country Status (1)

Country Link
BR (1) BR9510679A (en)

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Legal Events

Date Code Title Description
FB34 Technical and formal requirements: requirement - article 34 of industrial property law
FA14 Dismissal: dismissal - article 34 of industrial property law
B11T Dismissal of application maintained [chapter 11.20 patent gazette]