BR122017021068B1 - Method for producing a transgenic evergreen woody plant presenting increased growth measured by trunk dry weight as compared to its wild type - Google Patents

Abstract

The present invention relates to a novel and extensive analytical platform for selecting genes with a possible commercial phenotype from a large group of candidate genes using bioinformatics tools, est sequencing data, and DNA array. One aspect of the invention provides methods for producing a transgenic plant having increased growth compared to its wild type. The method comprises altering in the plant the level of a gene product of at least one gene specifically expressed during the different stages of wood formation. This can be done with transgenic methods or by specific breeding methods. other aspects of the invention provide a plant and wood and plant progeny cell having modulated gene expression according to the invention. other aspects relate to a DNA construct comprising a nucleotide sequence of the invention and a plant cell or plant progeny comprising the DNA construct.

Description

METHOD TO PRODUCE A TRANSGENIC PERENNIAL WOOL PLANT PRESENTING AN INCREASED GROWTH MEASURED BY THE DRY WEIGHT OF THE STEM IN COMPARISON WITH ITS WILD TYPE.

[001] Divided from PI0821492-1, deposited on 12/18/2008. Technical Field of the Invention [002] The present invention refers, in general, to the field of molecular biology and refers to a method to improve the growth characteristics of plants. More specifically, the invention relates to methods for phenotypically modifying plants, transgenic plants and plants obtained by a specific crossing method that have altered expression of a gene, resulting in a modified growth phenotype. The invention also provides constructs useful in the method of the invention. Furthermore, the invention relates to a plant cell or progeny of plants and wood produced by the plants according to the invention.

Background of the Invention [003] Currently, the primary objectives of forestry engineering and molecular reproduction are to improve the quality and yield of wood. The global demand for wood products grows around 1.7% per year, and this increase in wood consumption is occurring although the maximum sustainability rate for harvesting the world's forests has already been reached or exceeded. Therefore, there is a need to increase the production of planted wood in the world. Forest plantations may also have advantages such as harvesting carbon sequestration as a response to increased atmospheric CO2. Similarly, increased biomass production from non-woody plants is desirable to, for example, meet the demand for raw materials for energy production. The modification of specific processes during development

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2/362 to cellular in superior species is, therefore, of great commercial interest, not only when it comes to improving the properties of trees, but also those of other plants.

[004] The growth of vegetables through apical meristems results in the development of sets of primary tissues and in the elongation of the trunk and roots. In addition to this primary growth, tree species undergo secondary growth and produce secondary wood from exchange. Secondary growth increases the circumference of the trunk and roots.

[005] Perennial plants like long-lived trees have a lifestyle considerably different from annual plants like Arabidopsis, due to the fact that perennial plants like trees have an indeterminate growth, while plants like Arabidopsis have terminal growth when the plant blooms. The final size of an Arabidopsis plant is, in many ways, dependent on the program of development from germination to flowering and seed placement. An example is that any change in the timing of these events can drastically change the size of the plant.

[006] Perennial plants also have cycles of active growth and dormancy. During active growth, the leaves perform photosynthesis to capture energy, which is then used to trigger the various cellular processes. The fixed carbon that is converted into sucrose is transferred to the tissues of the trunk and apical bud, where it is stored during the dormant state initially as starch and later as sucrose. As growth resumes after dormancy, sucrose is shifted to tissues that are actively growing as initial reactivation steps occur before photosynthesis begins. Similar to nitrogen, amino acids

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3/362 are also displaced to the apical and trunk tissues and stored as storage proteins during dormancy and broken down as growth begins. Thus, the life cycle of long-lived trees differs significantly from annual trees, which often shift carbon and nitrogen to the seeds. Because of these differences between perennials such as trees and annual crops, the determinants of yield and the ability to measure them are likely to be very different. In fact, in several instances, a model system like Populus tremula x tremuloides is much better for reliably finding genes that can be used to increase biomass production. For example, for annual harvests, seed size / yield has been proposed to be a measure of plant size and productivity, but this is not likely to be true, as perennial plants such as trees take several years to flower and, thus, seed generation is the only indicator, if any, of the growing conditions that prevail during the year that the plant bloomed. Thus, direct translation of surveys and annual crop findings are unlikely to be useful for trees.

[007] A very important part of tree biomass is present in the tissues of the trunk. This accumulation of biomass is a result of leaf photosynthesis and nitrogen acquisition and redistribution of nutrients to various cellular processes. Thus, leaf size, leaf photosynthesis, and the size of the capacity to acquire nitrogen from the root system can be important actors in determining plant productivity and biomass production. However, none of these alone can be responsible for all biomass production. For example, leaf size is not always related to biomass as a large variation in leaf size can be found. Furthermore, the ability to

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4/362 adapting to stress is an important determinant of biomass production. Thus, there are several factors that need to be changed in order to improve the production of biomass in trees.

[008] Furthermore, the density of wood is an important feature in the improved production of biomass, a higher density of wood gives less volume to be transported and contains more energy per volume. Thus, an increased density is of interest even if the total biomass is not greater. Density is also important to show that increased metric growth in height and diameter is not coupled with a decrease in wood density.

[009] One way to increase growth is to learn more about the function of the gene and use that information to increase growth and biomass production. Such knowledge of the function of genes and the ways to use the knowledge are described in this patent.

[0010] Most genes have already been identified in several plants such as Arabidopsis thaliana (Arabidopsis Genome Initiative 2000) and Populus tremula x tremuloides (Sterky et al. 2004) and Populus tríchocarpa (Tuskan et al. 2006).

[0011] Hertzberg et al. 2001 and Schrader et al. 2005 used the transcriptional profile to reveal a transcriptional hierarchy for thousands of genes during xylem development, as well as to provide expression data. Such data can facilitate the further elucidation of many genes with unknown function (White et al. 1999; Aharoni et al. 2000).

[0012] One problem that remains is how to identify the genes that are potentially most important involved in regulating cell division and other growth-related processes. In the present invention, we examined several transcription factors paPetição 870170074044, of 29/09/2017, p. 16/378

5/362 r use that resulted in an unexpected increase in growth when overexpressed. The reason for selecting transcription factors for analysis is known that they are part of the regulators of many, if not most, processes in living organisms including plants. It can be predicted that Arabidopsis thaliana contains 1500 different transcription factors that can be divided into about 30 subclasses based on sequence homologies (Riechmann et al. 2000). The function that a certain transcription factor has in a plant is strongly linked to which gene it regulates, for example, although transcription factors in a subgroup of transcription factors such as those in the MYB class are similar, it is known that they regulate several processes different in vegetables. Transcription factors are proteins that regulate gene transcription by suppressing or activating the initiation of transcription of specific genes or regions of the genome that contain different genes.

[0013] Specific labeling of transcription factors in plants to find genes that can be used to change the characteristics of plants has already been done. For example, in WO 02/15675, a large number of transcription factors have been analyzed and the possible use for many of them has been mentioned. US2007 / 0039070 describes and lists a large number of Eucalyptus and Pinus radiata transcription factor genes and speculates on the use of such genes. Here, specific transcription factors were presented that have an industrially relevant effect in substantially increasing growth, supported by experimental data.

[0014] While it is obvious that the results of EST programs, genome sequencing and expression studies using DNA array technology can verify when and where a gene is expressed, it is rarely possible to clarify the biological and / or technical function of a gene only from these types of analytical tools.

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In order to analyze and verify the function of the gene, a functional characterization must be carried out, for example, by inactivating the gene and / or overexpressing the gene. However, in order to be able to identify genes with interesting and often unexpected commercial characteristics, candidate genes must be evaluated based on functional analysis and measurement of growth growth with different criteria.

[0015] MYB transcription factors. One of the genes presented here (SEQ ID NO: 12) belongs to the MYB class of transcription factors. The MYB family of transcription factors is expected to have about 180 members in Arabidopsis (Riechmann et al. 2000). Several different functions have been found for MYB genes in vegetables (Jin and Martin 1999). More specifically, according to our knowledge, it has not been shown that genes closely related to SEQ ID NO: 12 are involved in the regulation of growth rates and biomass production. US2003101481 and Katou et al.2005 says that the closely related genes AT2G101060 and AB192880 are involved in biotic stress responses.

[0016] SET domain transcription factors (Ng et al. 2007). One of the genes presented here SEQ ID NO: 11 belongs to the SET domain class of transcription factors. SET domain proteins regulate transcription by modulating the chromatin structure. The Arabidopsis genome is known to contain at least 29 active domain set proteins. As far as is known, it has not been shown that genes closely related to SEQ ID NO: 11 are involved in the regulation of growth rates and biomass production.

[0017] The bHLH class of transcription regulators is a large group of transcription factors in plants, for example, Arabidopsis thaliana is predicted to contain about 139 members (Riechmann

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7/362 et al. 2000). It implies that bHLH proteins are involved in several different processes, see Xiaoxing et al. 2006 for an overview of rice. One of the genes presented here SEQ ID NO: 10 belongs to the bHLH class of transcription factors. As far as is known, it has not been shown that genes closely related to SEQ ID NO: 10 are involved in the regulation of biomass growth and production rates.

[0018] The SEQ ID NO: 9 gene belongs to the Homeobox class. The Arabidopsis thaliana counterpart closest to the overexpressed gene with the TF0013 construct is predicted to be AT1G23380. Overexpression of a Solanum tuberosum related to the overexpressed gene with construct TF0013 decreases growth, length between nodes and leaf size (US 7265263). Overexpression of a related Arabidopsis thaliana homologous to the overexpressed gene with construct TF0013 alters the leaf morphology (US 7265263, US 20070022495 and WO 01036444). The use to increase biomass yield and production by altering the expression level of an overexpressed gene with the TF0013 construct was previously unknown.

[0019] The IAA / AUX group of transcription factors is a small group of transcription factors found mainly in vegetables (26 members predicted in Arabidopsis by Riechmann et al. 2000). The gene that corresponds to SEQ ID NO: 13 belongs to this group and is described by Moyle et al. 2002. As far as is known, it has not been shown that genes closely related to SEQ ID NO: 13 are involved in the regulation of growth rates and biomass production.

[0020] The WRKY gene family group. The WRKY transcription factor family is a large family of genes in vegetables. Rice is predicted to have more than 90 members and Arabi is predicted

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8/362 dopsis has more than 74 members (Ulker and Somssich 2004). One of the functions that have been most associated with the WRKY genes is the signaling of defense against pathogens and injuries, but also signaling coupled with abiotic stress and resistance against abiotic and biotic stresses.

[0021] Eight of the genes presented here belong to the WRKY class of transcription factors. SEQ ID NO: 4 and SEQ ID NO: 7 belong to a subgroup of WRKY genes. As far as is known, it has not been shown that genes closely related to SEQ ID NO: 4 and SEQ ID NO: 7 are involved in the regulation of growth rates and biomass production.

[0022] SEQ ID NO: 1 belongs to another subgroup of WRKY genes. An Arabidopsis thaliana closely related homologous (AT2G23320) to the SEQ ID NO: 1 gene is believed to be involved in C / N sensing (US 20060272060), leaf size change (US 7238860, US 20030226173, US 20040019927 and WO 02015675) and in the alteration of the protein content in the seeds (US 20030226173). AT2G23320 is also believed to be involved in the reaction and adaptation to peroxide stress according to patent application WO 04087952. US 20040019927, US 7238860, US 20030226173 and WO 02015675 mention the AT2G23320 gene in combination with the increase in leaf size and height increase, and they speculate that the overexpression of this gene can be used to increase the growth and production of biomass. It has been shown here that SEQ ID NO: 1 can be used on trees to increase growth to an industrially significant level.

[0023] SEQ ID NO: 6 belongs to a subgroup of WRKY genes that are related to the group to which SEQ ID NO: 1 belongs, but is clearly different from this group of genes. Genes are known to

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9/362 closely related to this gene are negative regulators of basal resistance in Arabidopsis thaliana (Journot-Catalino et al. 2006). The closely related AT4G31550 gene is believed to be related to seed prenyl lipid levels and seed lutein levels (US 20060195944 and US 20070022495 and WO 01035727). Another Arabidopsis thaliana counterpart predicted AT2G24570 to SEQ ID NO: 6 is believed to be involved in C / N sensing (US 20070022495 and US 20060272060). As far as is known, it has not been shown that genes closely related to SEQ ID NO: 6 are involved in the regulation of biomass growth and production rates.

[0024] SEQ ID NO: 2 belongs to another subgroup of WRKY genes. As far as is known, it has not been shown that genes closely related to SEQ ID NO: 2 are involved in the regulation of growth rates and biomass production.

[0025] SEQ ID NO: 3 and SEQ ID NO: 5 refer to a large group of WRKY genes that contain 2 WRKY domains. Some homologues related to SEQ ID NO: 3 and SEQ ID NO: 5 that contain two WRKY domains are believed to be involved in altering the seed generation and the number of flowers of Oryza sativa according to patent application WO 2007003409. The use to increase biomass growth and production by changing expression levels was unknown.

[0026] SEQ ID NO: 8 belongs to another subgroup of WRKY genes. The closely related Arabidopsis thaliana gene AT4G23810 is known to reduce plant size and is involved in altering protein content (US 20030226173). Another related Arabidopsis thaliana counterpart (AT5G24110) is known to be involved in altering the protein content of the seed and inducing premature flowering (US 20030226173). As far as is known,

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10/362 it has not been shown that genes closely related to SEQ ID NO: 8 are involved in the regulation of growth rates and biomass production.

Summary of the Invention [0027] The present invention relates to new genes that can be used to increase growth. Genes are found through the use of an analytical platform that focuses on analyzing growth behavior in several criteria. The invention provides methods for the production of a transgenic plant by changing the expression of one or more genes selected from a group of genes that meets such criteria. Thus, the invention relates to methods for phenotypically modifying transgenic plants and plants and plants obtained by a specific crossing method that have altered expression of a gene, resulting in a modified growth phenotype. The invention also provides constructs useful in the method of the invention. Furthermore, the invention relates to a plant cell or plant progeny of plants and wood produced with unexpectedly good properties by the plants according to the invention. A number of genes analyzed using the analytical platform show commercially interesting and often unexpected characteristics. Thus, an aspect of the present invention provides a method for producing a plant that has unexpectedly high growth compared to its wild type, comprising altering (increasing) the level of a genetic product in at least one gene that belongs to the plant. to one of the sequences of transcription factors SEQ ID NO: 1 - 13, 97 - 115.

[0028] The increase in growth can be observed when comparing a group of transgenic plants grown for 8 weeks in a greenhouse under an 18 hour photoperiod, a temperature of 22 ° C / 15 ° C (day / night) and a Weilbulls weekly fertilization

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Rika S NPK 7-1-5 diluted from 1 to 100 with a group of wild type plants grown under identical conditions.

[0029] Another aspect of the invention provides a plant cell or plant progeny from a transgenic plant or a plant with intentionally modified (increased) levels of one of the SEQ ID NO: 1 - 13, 97-115 genes according to the invention and comprising a recombinant polynucleotide.

[0030] Another aspect of the invention also provides biomass and products produced by a plant that intentionally has the characteristics described above.

[0031] Furthermore, another aspect of the invention provides a DNA construct that comprises at least one sequence as described here.

[0032] Finally, one aspect of the invention provides a plant cell or plant progeny that comprises the construction of DNA according to the invention.

Description of Figure [0033] Figure 1 shows an example of the height growth curve with four different linear regression lines shown, the black linear regression line shows the highest height growth rate.

Detailed Description of the Invention

Definitions [0034] Before discussing the present invention in more detail, the following terms and conventions will be defined:

[0035] The term transgenic plant refers to a plant that contains genetic material not found in a wild type plant of the same species, variety or cultivar. The genetic material can include a transgene, an insertional mutagenesis event (such as T-DNA or transposon insertional mutagenesis), a sequence

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12/362 activation tag, a mutated sequence, a homologous recombination event or a sequence modified by chimeroplasty. Typically, exogenous genetic material was introduced into the plant by human manipulation. The term also refers to plants where the genetic material has been inserted to function as a selection marker. Examples of such selection markers include kanamycin, hygromycin, phosphoinotricin, clorsulfron, methotrexate, gentamicin, spectinomycin, imidazolinones, d-amino acids and glyphosate.

[0036] In the present context, the term growth includes primary growth, including an elongation in the trunk and roots, as well as the secondary growth of a plant, including the production of secondary tissue, wood, from the exchange and increase in the circumference of the trunk and roots. Thus, the term increased growth refers, in the present context, to an increased growth of a transgenic plant in relation to the wild type plant from which the transgenic plant is derived, when grown under the same cultivation conditions. As described below, a transgenic plant is characterized by the fact that it has increased growth if the plant meets at least one of the growth difference selection criteria as defined in the Examples that follow.

[0037] The term phenotype refers, in the present context, to the total physical appearance of an individual plant, such as growth. Examples of different growth phenotypes used in the present context are listed in table 1.2 below.

[0038] The term gene refers, in general, to any segment of DNA associated with a biological function. The genes include coding sequences and / or regulatory sequences required for their expression. Genes also include nucleic acid segments of unexpressed DNA that, for example, form sequences

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13/362 recognition for other proteins (for example, promoter, enhancer or other regulatory regions). Genes can be obtained from a variety of sources, including cloning from a source of interest or synthesis from predicted or known sequence information, and can include sequences designed to have the desired parameters.

[0039] Overexpression refers to the expression of a polypeptide or protein encoded by a DNA of SEQ ID NO: 1-13, 97-115 or similar sequences introduced into a host cell, where such polypeptide or protein is or is not normally present in the host cell, or where such a polypeptide or protein is present in such a host cell at a higher level than that normally expressed from the endogenous gene encoding such a polypeptide or protein.

[0040] Protein overexpression of the present invention can be achieved, first, by constructing a chimeric gene in which the coding region is operably linked to a promoter capable of directing the expression of a gene in the desired tissues at the desired stage of development . The chimeric gene can comprise promoter sequences and leading translation sequences derived from the same genes. Non-3 'coding sequences that encode transcription termination signals can also be provided. The present chimeric gene can also comprise one or more introns in order to facilitate expression of the gene. A suitable promoter can be the CaMV 35 S promoter which can be used with Agrobacterium as a vector.

[0041] The term interference RNA or RNAi generally refers to a process in which a double-stranded RNA molecule or a short RNA clamp that changes the expression of the nucleic acid sequence with which they share homology substantial or

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14/362 total.

[0042] The term RNAi infrarregulation refers to the reduction in the expression of a nucleic acid sequence mediated by one or more RNAi species. The term RNAi species refers to a distinct RNA sequence that generates RNAi.

[0043] The term photoperiod refers to the daily cycle of light and darkness.

[0044] The terms nucleic acid constructs, DNA and vector constructs refer to a genetic sequence used to transform plants or other organisms. Nucleic acid construction or DNA construction may be able to target, in a transformed plant, the expression of a protein or nucleic acid sequence, such as an antisense RNA. Typically, such nucleic acid constructs or DNA constructs comprise at least one region that encodes a desired gene product or a desired nucleic acid product operably linked to 3 'and 5' transcriptional regulatory elements. In some embodiments, such nucleic acid constructs or DNA constructs are chimeric, that is, they consist of a mixture of sequences from different sources. However, non-chimeric nucleic acid or DNA constructs can also be used in the present invention.

[0045] The term recombinant when used with respect to, for example, a cell, nucleotide, vector, protein or polypeptide typically indicates that the cell, nucleotide or vector has been modified by the introduction of a heterologous (or exogenous) nucleic acid or by alteration of a native nucleic acid, or that the protein or nucleic acid has been altered by the introduction of a heterologous amino acid, or that the cell is derived from a cell thus modified. Recombinant cells that express nucleic acid sequences (for example,

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15/362 genes) that are not found in the native (non-recombinant) form of the cell or that express nucleic acid sequences (eg, genes) that would be abnormally expressed, underexpressed or that simply would not be expressed. The term recombinant when used to refer to cells, indicates that the cell replicates a heterologous nucleic acid, or expresses a peptide or protein encoded by a heterologous nucleic acid. Recombinant cells can contain genes that are not found in the cell's native (non-recombinant) form. Recombinant cells can also contain genes found in the native form of the cell in which the genes are modified and reintroduced into the cell by artificial means. The term also encompasses cells that contain a nucleic acid endogenous to the cell that has been modified without removing a nucleic acid from the cell; such modifications include those obtained by gene replacement, site-specific mutation and related techniques.

[0046] The term nucleic acid sequence refers to a polymer of deoxyribonucleotides or ribonucleotides in the form of single-stranded or double-stranded. Unless specifically limited, the term encompasses nucleic acid sequences that contain known analogs of natural nucleotides that have binding properties similar to that of the reference nucleic acid, and that are metabolized in a similar way to naturally occurring nucleotides. Unless otherwise stated, a particular nucleic acid sequence also implicitly encompasses conservatively modified variants of these (e.g., degenerate codon substitutions) and complementary sequences, as well as the sequences explicitly indicated.

A polynucleotide is a sequence of nucleic acids that comprises a plurality of polymerized nucleotide residues, for example, at least about 15 nucleotide residues

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16/362 consecutive polymerized deos, optionally, at least about 30 consecutive nucleotides, at least about 50 consecutive nucleotides. In many cases, a polynucleotide comprises a sequence of nucleotides that encodes a polypeptide (or protein) or a domain or fragment thereof. In addition, the polynucleotide can comprise a promoter, an intron, an enhancer region, a polyadenylation site, a translation initiation site, 3 'or 5' untranslated regions, a reporter gene, a selectable marker and the like. The polynucleotide can be single-stranded or double-stranded DNA or RNA. Optionally, the polynucleotide comprises modified bases or a modified framework. The polynucleotide can be, for example, genomic DNA or RNA, a transcript (such as an mRNA), a cDNA, a PCR product, cloned DNA, synthetic DNA or RNA, or the like. The polynucleotide can comprise a sequence in the sense or antisense orientation.

[0048] The term polypeptide is used, in general, to define linear chains of amino acid residues, including naturally occurring ones and synthetic analogues thereof.

[0049] In the context of the present invention, complementary refers to the ability for precise pairing between two nucleotide sequences with one another. For example, if a nucleotide at a certain position in an oligonucleotide is capable of hydrogen bonding to a nucleotide at the corresponding position in a DNA or RNA molecule, then the oligonucleotide and DNA or RNA are considered complementary to each other in that position. The DNA and RNA strands are considered complementary to each other when a sufficient number of nucleotides in the oligonucleotide are able to form hydrogen bonds with the corresponding nucleotides in the target DNA or RNA to allow the formation of a stable complex.

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17/362 [0050] In the present context the expressions complementary sequence and complement thus also refer to nucleotide sequences that will ring a nucleic acid molecule of the invention under stringent conditions.

[0051] The term stringent conditions refers to general conditions of high, low or weak stringency.

[0052] The term stringency is well known in the art and is used to refer to the conditions (temperature, ionic strength and the presence of other compounds such as organic solvents) under which nucleic acid hybridizations are conducted. With conditions of high stringency, the pairing of nucleic acid bases will only occur between fragments of nucleic acids that have a high frequency of complementary base sequences, when compared to conditions of low or weak stringency. Suitable conditions for the hybridization test involve pre-soaking in 5xSSC and pre-hybridization for 1 hour at about 40 ° C in a 20% formamide solution, 5xDenhardt solution, 50mM sodium phosphate, pH 6.8 and 50 mg of denatured calf thymus DNA, followed by hybridization in the same solution supplemented with 100mM ATP for 18 hours at about 40 ° C, followed by washing the filter three times in 2xSSC, 0.2% SDS at 40 ° C for 30 minutes (low stringency), preferably at 50 ° C (medium stringency), more preferably at 65 ° C (high stringency), even more preferably at about 75 ° C (very high stringency). More details about the hybridization method can be described by Sambrook et al, Molecular Cloning:. A Laboratory Manual, 2nd Edition, Cold Spring Harbor. 1989.

[0053] The terms hybridization and hybridization are used in general to designate the association between complementary or partially complementary nucleic acid sequences, as in a reverse process of the denaturation process by which they are

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18/362 stops. Hybridization occurs by hydrogen bonding, which can be Watson-Crick, Hoogste, reverse Hoogsten hydrogen bonding, etc., between complementary nucleotide or nucleoside bases. The four nucleobases commonly found in DNA are G, A, T and C, where G pairs with C and A pairs with T. In RNA, T is replaced by uracil (U), which then pairs with A. The chemical groups in nucleobases that participate in the standard duplex formation constitute the Watson-Crick face. Hoogsten showed, some years later, that the purine nucleobases (G and A), in addition to their Watson-Crick faces, have a Hoogsten face that can be recognized from outside a duplex, and used to bind pyrimidine oligonucleotides via hydrogen jumper, thus forming a triple helix structure.

[0054] A subsequence or fragment is any part of an entire sequence. Thus, a fragment or a subsequence refers to a sequence of amino acids or nucleic acids that comprise a part of a longer sequence of amino acids (e.g., polypeptide) or nucleic acids (e.g., polynucleotide) respectively.

[0055] In the present context, the term homology indicates similarities between two amino acid sequences or between two nucleotide sequences, and is described by the sequence identity parameter.

[0056] The term sequence identity indicates a quantitative measure of a level of homology between two sequences of amino acids or between two sequences of nucleic acids of equal length. If the two sequences being compared do not have the same length, they must be aligned in order to obtain the best possible fit, allowing the insertion of gaps or, alternatively, truncation at the ends of the polynuPetition sequences 870170074044, from 29/09/2017 , p. 30/378

19/362 cleotides or nucleotide sequences. The sequence identity (N „fN <uf) 100 can be calculated as ^Nr *, where N _dif is the total number of non-identical residues in the two sequences when aligned and where N _ref is the number of residues in one of the sequences . Thus, the AGTCAGTC DNA sequence will have 75% sequence identity with the AATCAATC sequence (N _dif = 2 and N _ref = 8). A gap is counted as a non-identity of the specific residue, that is, the DNA sequence AGTGTC will have 75% sequence identity with the DNA sequence AGTCAGTC (N _dif = 2 and N _ref = 8).

[0057] With respect to all modalities of the invention that refer to nucleotide sequences, the percentage of sequence identity between one or more sequences can also be based on the alignments using the clustalW software (http: /www.ebi.ac .uk / clustalW / index.html) with default settings. For nucleotide sequence alignments, these settings are: Alignment = 3Dfull, Gap Open 10.00, Gap Ext. 0.20, Gap separation Dist. 4, DNA weight matrix: identity (IUB). Alternatively, the sequences can be analyzed using the DNASIS Max program and the comparison of the sequences can be done at www.paraliqn.orq. This service is based on two comparison algorithms called Smith-Waterman (SW) and ParAlign. The first algorithm was published by Smith and Waterman (1981) and is a well-established method that finds the optimal local alignment of two sequences. The other algorithm, ParAlign, is a heuristic method for aligning sequences; the details of the method are published in Rognes (2001). The default settings for the matrix of values and gap penalties, as well as E-values were used.

[0058] The phrase substantially identical or substantial identity in the context of two nucleic acids or polypeptides, refers to

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20/362 two or more sequences or subsequences that have at least about 60%, 70%, 75%, preferably 80% or 85%, more preferably 90%, 91%, 92%, 93%, 94%, 95% , 96%, 97%, 98%, 99%, 99.5% or more of percent amino acid or nucleotide residue identity, respectively, when compared and aligned for maximum match, as measured using one of the following sequence comparison algorithms or by visual inspection. In certain respects, substantial identity exists over a region of amino acid sequences of at least about 50 residues in length, for example, at least about 100, 110, 120, 125, 130, 135, 140, 145, 150 , 155, 160 or 165 amino acid residues. In certain respects, substantial identity exists over a region of the amino acid sequences of at least about 150 amino acid residues, for example, at least about 200, 250, 300, 330, 360, 375, 400, 425, 450 , 460, 480, 500, 600, 700, 800 as at least about 900 nucleotides or as at least about 1 kb, 1.1 kb, 1.2 kb, 1.3 kb, 1.4 kb, 1, 5 kb, 1.6 kb, 1.7 kb, 1.8 kb, 1.9 kb, 2 kb, 2.1 kb, 2.2 kb, 2.3 kb, 2.4 kb, 2.5 kb , 2.6 kb, 2.7 kb, 2.8 kb, 2.9 kb or as at least about 3 kb. In some respects, the amino acid or nucleic acid sequences are substantially identical over the entire length of the polypeptide sequence or the corresponding coding region.

[0059] The term conservative substitutions is in the group of basic amino acids (arginine, lysine and histidine), acidic amino acids (glutamic acid and aspartic acid), polar amino acids (glutamine and asparagine), hydrophobic amino acids (leucine, isoleucine, valine and methionine) , aromatic amino acids (phenylalanine, tryptophan and tyrosine) and small amino acids (glycine, alanine, serine and threonine). Amino acid substitutions that do not normally alter specific activity are known in the art and described by, for example, Neurath

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21/362 and Hill, 1979. The most common occurrence exchanges are Ala / Ser, Val / lle, Asp / Glu, Thr / Ser, Ala / Gly, Ala / Thr, Ser / Asn, Ala / Val, Ser / Gly , Tyr / Phe, Ala / Pro, Lys / Arg, Asp / Asn, Leu / lle, Leu / Val, Ala / Glu, and Asp / Gly as well as those in reverse.

[0060] The term conservatively substituted variant, as used here, refers to a variant of a nucleotide sequence that comprises one or more conservative substitutions.

[0061] In general and in the present context, the term silent substitution refers to a base substitution that does not affect the sense of a codon and, thus, has no effect on the structure of the polypeptide. A technician in the field knows that silent substitutions are possible due to the degeneration of the genetic code.

[0062] The term conserved domain refers to a sequence of amino acids in a polypeptide or a sequence of nucleotides in DNA or RNA that is similar between different species. A known set of conserved sequences is represented by a consensus sequence. Amino acid motifs are often composed of conserved sequences. In addition, the term conserved sequences refers to a base sequence in a nucleic acid molecule or an amino acid sequence in a protein that has remained essentially unchanged throughout evolution. A consensus sequence is defined in terms of an idealized sequence that represents the base most often present at each position in a protein. A consensus sequence is identified by aligning all known examples of a sequence of nucleic acids or a protein in order to maximize their sequence identities. For a sequence to be accepted as a consensus sequence, each particular amino acid or base must be reasonably predominant in its position and most sequences must relate to the consensus sequence for just

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22/362 some substitutions, such as 1 or 2.

[0063] A homolog can also be in the form of an insertion variant of a protein, that is, where one or more amino acid residues are introduced at a predetermined site on a protein. The inserts may comprise N-terminal and / or C-terminal fusions as well as single or multiple amino acid intra-sequence insertions. In general, insertions within the amino acid sequences will be smaller than N- or C-terminal fusions, on the order of about 1 to 10 residues. Examples of C- or N-terminal fusions proteins or peptides include the binding domain or the activation domain of a transcriptional activator as used in the yeast two hybrid system, phage coat proteins, (histidine) marker -6, glutathione S-transferase marker, protein A, maltose-binding protein, dihydrofolate reductase, epitope of marker-100, epitope c-myc, epitope FLAG®, lacZ, CMP (calmodulin binding peptide), epitope HA , epitope of the Ceo epitope VSV protein.

[0064] Homologues in the form of deletion variants of a protein are characterized by the removal of one or more amino acids from the protein.

[0065] Homologues in the form of variants of adding a protein are characterized by the addition of one or more amino acids to the protein, where the addition can be at the end of the sequence.

[0066] Amino acid variants of a protein can be readily made using synthetic peptide techniques well known in the art, such as solid phase peptide synthesis and the like, or by recombinant DNA manipulations. Methods for manipulating DNA sequences to produce variants of substitution, deletion or addition of a protein are well known in the art. For example, techniques for making substitution mutations in

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23/362 predetermined DNA sites are well known to those skilled in the art and include M13 mutagenesis, T7-Gen in vitro mutagenesis (USB, Cleveland, Ohio), QuickChange site-directed mutagenesis (Stratagene, San Diego, California), targeted mutagenesis the site mediated by PCR or other site-directed mutagenesis protocols.

[0067] The terms Orthological and Parallel sequences are also types of homologous sequences as described above. Several different methods are known to those skilled in the art to identify and define these functionally homologous sequences. Three general methods for defining orthologists and similars are described; an orthologist, parallelist or homologue can be identified by one or more of the methods described below.

[0068] Orthologists and parallels are evolutionarily related genes that have similar sequences and similar functions. Orthologists are structurally related genes in different species that are derived by a speciation event. Parallels are structurally related genes within a single species that are derived from a duplication event.

[0069] Within a single plant species, duplication of genes can generate two copies of a particular gene, generating two or more genes with similar sequences and often similar functions known as parallels. Thus, a paragon is a similar gene formed by duplication in the same species. Parallels are usually grouped in the same category (a group of similar genes) when the phylogeny of a family of genes is analyzed using programs like CLUSTAL (Thompson et al. ·, Higgins et al.). Similar gene groups can also be identified with the pair-oriented BLAST analysis (Feng and Doolittle). For example, a set of very similar MADS domain transcription factors from Arabidopsis with

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24/362 shares a common function at the time of flowering (Ratcliffe et al.), And a group of AP2 domain transcription factors very similar to Arabidopsis are involved in plant tolerance to freezing (Gilmour et al.). The analysis of groups of similar genes with similar function that are in the same cycad may yield subsequences that are particular to the cycad. These subsequences, known as consensus sequences, can be used not only to define the sequences within each member, but also to define the functions of these genes; genes in a cyiate may contain parallel sequences or ortholog sequences that share the same function (see also, for example, Mount (2001), in Bioinformatics: Sequence and Genome Analysis Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, page 543) .

[0070] Speciation, the production of new species from parent species, can also generate two or more genes with similar sequences and similar function. These genes, called orthologists, often have an identical function in their host plants and are often interchangeable between species without losing their function. Since plants have common ancestors, many genes in one plant species will have corresponding ortholog genes in another plant species. Once a phylogenetic tree for a family of genes of a species has been built using a program like CLUSTAL, potential ortholog sequences can be placed on the phylogenetic tree and their relationship to the genes of the species of interest can be determined. Orthologous sequences can also be identified by a reciprocal BLAST strategy. Once an orthologous sequence has been identified, the function of the orthologist can be deduced from the identified function of the reference sequence.

[0071] Orthologous genes from different organisms have fun

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25/362 highly conserved tions and, very often, essentially identical functions (Lee et al. And Remm et al.). Parallel genes, which diverged through genetic duplication, may retain functions similar to the encoded proteins. In such cases, the parallels can be used interchangeably with respect to certain embodiments of the present invention (for example, transgenic expression of a coding sequence). An example of such highly related parallels is the CBF family, with three well-defined members in Arabidopsis and at least one orthologist in Brassica napus, all of which control the pathways involved in both freezing and drought stress (Gilmour et al. And Jaglo et al.).

[0072] The following references represent a small sample of the many studies that demonstrate that conserved transcription factor genes from different species are likely to work in a similar way (ie, regulate similar target sequences and control the same characteristics), and that transcription can be transformed into several species in order to give them or improve characteristics.

[0073] (1) The Arabidopsis NPR1 gene regulates acquired systemic resistance (SAR); overexpression of NPR1 leads to greater resistance in Arabidopsis. When either NPR1 of Arabidopsis or the orthologist of NPR1 of rice is overexpressed in rice (which, as a monocot, is distinct from Arabidopsis), the challenge with the malignant bacterial rice pathogen Xanthomas oryzae pv. Oryzae, the transgenic plants showed improved resistance (Chern et al.). NPR1 acts by activating the expression of genes from transcription factors such as TGA2 (Fan and Dong).

[0074] (2) E2F genes are involved in the transcription of plant genes for the proliferation of cellular nuclear antigen (PCNA). Vegetable E2Fs share a high degree of similarity in sequence

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26/362 of amino acids between monocots and dicots, and are even similar to the conserved domains of animal E2Fs. Such conservation indicates a functional similarity between plant and animal E2Fs. E2F transcription factors that regulate meristem development act via cis elements in common, and regulate related genes (PCNA) (Kosugi and Ohashi).

[0075] The term closely related genes is used for genes that are orthologists and parallels.

[0076] The term promoter, as used here, refers to a region of sequence determinants located upstream from the beginning of a gene transcription and which are involved in the recognition and binding of RNA polymerase and other proteins to initiate and modulate the transcript. Useful promoters in vegetables need not be of vegetable origin. A basal promoter is the minimum sequence necessary for the assembly of a transcription complex necessary for initiation of transcription. Baseline promoters often include a TATA box element normally located between 15 and 35 nucleotides above the transcription initiation site. Baseline promoters also sometimes include a CCAAT box element (typically, a CCAAT sequence) and / or a GGGCG sequence, usually located between 40 and 200 nucleotides, preferably between 60 and 120 nucleotides above the transcription start site.

[0077] The promoters that are referred to here as constitutive promoters actively promote transcription over most, but not necessarily all, environmental conditions and developmental states or cell differentiation. Examples of constitutive promoters include the cauliflower mosaic virus (CaMV) 35S transcription initiation region and the T or 2 'promoter derived from Agrobacterium tumefaciens T-DNA, and other regions of

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27/362 initiation of transcription of several plant genes, such as the cornstarch ubiquitin-1 promoter, known to those skilled in the art. Organ-specific promoters may be, for example, a tissue-soaked storage promoter such as seed, potato and fruit tubers, or metabolic-soaked tissues such as meristems, a seed-specific promoter such as a glutelin, prolamine, globulin or rice albumin, a Vicia faba promoter of the B4 vegetable and the unknown Vicia faba seed protein gene, a promoter of a seed body oil protein, the promoter of BrassA napus storage protein, or any other specific seed promoter known in the art as, for example, described in WO 91/14772. In addition, the promoter may be a leaf-specific promoter such as the rbcs rice or tomato promoter, the chlorella virus adenine methyltransferase gene promoter, or the rice aldP gene promoter, or an injury-inducible promoter with the potato pin2.

[0078] An inducible promoter in the context of the present invention refers to a promoter that is regulated under certain conditions, such as light, chemical concentration, protein concentration, conditions in an organism, cell or organelle, etc. An example of an inducible promoter is the HSP promoter and PARSK1, the promoter of the Arabidopsis gene which encodes a serine threonine kinase enzyme and which is induced by dehydration, abscisic acid and sodium chloride. Essentially, expression under the control of an inducible promoter is switched on or increased in response to an applied stimulus. The nature of the stimulus varies among promoters and may include the environmental factors mentioned above. Whatever the level of expression in the absence of a stimulus, expression from any inducible promoter is increased in the presence of the correct stimulus.

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28/362 [0079] As used here, the term fabric specific refers to a characteristic of a particular fabric that is not generally found in all fabrics, or can be found exclusively in a fabric of interest. In the present application, tissue specific is used in reference to a gene regulator element (promoter or more enhancer and / or silencer), the gene it encodes or the polypeptide product of such a gene. In the context of a gene regulatory element or tissue-specific promoter, the term means that the promoter (and also other regulatory elements such as silencing and / or enhancing elements) directs the transcription of a linked sequence in a cell of a lineage, tissue or cell type in particular, but is substantially inactive in cells or tissues that are not of that lineage, tissue or cell type. A tissue-specific promoter useful according to the invention is at least 5 times, 10 times, 25 times, 50 times, 100 times, 500 times or even 1,000 times more active in terms of transcription production in the particular tissue than it is in cells of other tissues or in transformed or malignant cells of the same lineage. In the context of a gene or the polypeptide product of a gene, the term tissue specific means that the polypeptide product of the gene is detectable in cells of that particular tissue or cell type, but is not substantially detectable in other certain cell types. Particularly relevant tissue-specific promoters include the promoter sequences expressed or active in the tissue that forms a plant's xylem. Examples of such promoters are the Lmp1, Lmx2, Lmx3, Lmx4 and Lmx5 promoters, described in WO 2004097024.

[0080] A terminator sequence refers to a section of the genetic sequence that marks the end of the gene or operon in genomic DNA for transcription. Terminating strings are recognized

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29/362 due to protein factors that cotranscriptionally cleave the nascent RNA in a polyadenylation signal, preventing further elongation of the transcript by RNA polymerase. A nucleic acid is operationally linked when it is placed in a functional relationship with another nucleic acid sequence. For example, a promoter or enhancer is operably linked to a coding sequence if it increases the transcription of the coding sequence. Operationally linked means that the DNA sequences being linked are typically contiguous and, when necessary to join two protein coding regions, contiguous and in the same reading frame. However, since enhancers generally work when they are separated from the promoter by several kilobases and the intronic sequences can be of varying length, some polynucleotide elements may be operationally linked, but not contiguous.

[0081] In the context of the present invention, the terms transformation and transformant are used interchangeably and as synonyms for transfecting and transfecting, respectively, to refer to the process of introducing DNA into a cell. DNA constructs, including at least the part of the gene or promoter of interest, can be introduced into host cells, which as stated above, can be individual cells, cells in culture, cells that are part of a host organism, a fertilized oocytic orgametophyte or an embryonic cell. By the term introduction, when used in reference to a host cell, it refers to standard procedures known in the art for introducing the DNA of a recombinant vector into the target host cell. Such procedures include, without limitation, transfection, infection, transformation, natural absorption, electroporation, biolistics and Agrobacterium.

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30/362 [0082] By regenerable cell is meant a plant cell from which an entire plant can be regenerated. It should be understood that a regenerable cell is a cell that has maintained its genetic potential, also known in the art as totipotency. It should also be understood that regenerable cells, when grown, may need the appropriate stimuli to express the total genetic potential of the source plant.

Production method of a transgenic plant [0083] In specific embodiments of the invention, advantageous plant phenotypes are generated by modifying, with respect to the corresponding wild type plant, the level of expression of candidate genes that have been evaluated and selected according to the criteria above . In accordance with these aspects a method is provided, which comprises altering in the plant, the level of a gene product of at least one gene comprising a nucleotide sequence selected from the group consisting of:

a) a nucleotide sequence of SEQ ID NO: 1-13, 97-115;

b) a nucleotide sequence that is at least 60% identical to a nucleotide sequence of SEQ ID NO: 1 - 13, 97 115;

c) a subsequence or fragment of a nucleotide sequence from a) or b).

[0084] This can be effect following the technically modified crossing method which comprises

i) select plant species that express at least one of the selected nucleotide sequences from the group consisting of

a) a nucleotide sequence of SEQ ID NO: 1-13, 97-115;

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b) a nucleotide sequence that is at least 60% identical to a nucleotide sequence of SEQ ID NO: 1 - 13, 97 115;

c) a subsequence or fragment of a nucleotide sequence from a) or b).

ii) cross a plant species selected in i) with the same or another plant species selected in i), iii) select plants with modulated expression of at least one of the selected nucleotide sequences from the group consisting of

d) a nucleotide sequence of SEQ ID NO: 1-13, 97-115;

e) a nucleotide sequence that is at least 60% identical to a nucleotide sequence of SEQ ID NO: 1 - 13, 97 115;

f) a subsequence or fragment of a nucleotide sequence of a) or b) compared to the plant species selected in i) iv) optionally crossing one or more plants obtained in iii) and selecting plants with modulated hair expression least one of the selected nucleotide sequences from the group consisting of

g) a nucleotide sequence of SEQ ID NO: 1-13, 97-115;

h) a nucleotide sequence that is at least 60% identical to a nucleotide sequence of SEQ ID NO: 1 - 13, 97 115;

i) a subsequence or fragment of a nucleotide sequence from a) or b). compared to the plant species selected in i) and / or plants obtained in iii).

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[0085] According to one aspect of the invention, a method is provided that comprises the following steps:

(i) providing an expression vector comprising the nucleotide sequence selected from the group consisting of

a) a nucleotide sequence of SEQ ID NO: 1-13, 97-115; or

b) a nucleotide sequence that is at least 60% identical to a nucleotide sequence of SEQ ID NO: 1 - 13, 97 115; or

c) a subsequence or fragment of a nucleotide sequence of a) or b) and

d) at least one regulatory element operatively linked to the polynucleotide sequence, wherein such at least one regulatory element controls the expression of the polynucleotide sequence in the target plant;

(ii) introducing the expression vector in at least one plant; and (iii) select at least one transgenic plant that has modulated biomass and / or growth compared to the wild type.

[0086] The sequences specified by SEQ ID 1 numbers 13, 97 - 115 represent sequences of the candidate genes as predicted from Populus trichocarpa and SEQ ID numbers 73 - 95 as cloned from hybrid black beech. As one skilled in the art will understand, additional sequences of these 5 'as well as 3' genes to the sequence described in SEQ ID NO: 73 - 95 are readily obtained through conventional cloning techniques, such as those described by Sambrook et al.

[0087] According to a modality, the modulated expression is carried out when introducing a genetic modification, preferably,

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33/362 at the site of a gene encoding a polypeptide comprising SEQ ID NO: 1 - 13, 97 - 115 or a homologue of such a polypeptide.

[0088] The modification can be carried out by one of: TDNA activation, TILLING, homologous recombination, site-directed mutagenesis or targeted reproduction using one or more of SEQ ID NO: 1-13, 97-115 as markers at any stage of the process.

[0089] The effect of modulation can be increased yield in growth and / or biomass.

Nucleic acid constructions [0090] In accordance with more particular embodiments of the invention, the method comprises the step of providing a nucleic acid construct, such as a recombinant DNA construct, comprising the nucleotide sequence selected from the group consisting of:

a) a nucleotide sequence of SEQ ID NO: 1-13, 97-115;

b) a nucleotide sequence complementary to a nucleotide sequence of a);

c) a subsequence or fragment of a nucleotide sequence of b) or c).

d) a nucleic acid sequence that is at least 60% identical to any of the sequences in a), b) and c); and

e) a nucleotide sequence that hybridizes under stringent conditions to a nucleotide sequence of a), b) or c).

[0091] In additional embodiments of the invention, the nucleic acid sequence in c) or g) is at least 65% identical to any of the sequences in a), c), d), e) or f), as well as by at least 70% identical, at least 75% identical, at least 80% identical, at least 85% identical, at least 87% identical, at least 90% identical,

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34/362 at least 95% identical, at least 97% identical, at least 98% identical, at least 99% identical, at least 99.5% identical to any of the sequences in a), c), d), e) or f).

[0092] In preferred embodiments of that aspect of the invention, the nucleotide sequence of a) is selected from the group consisting of SEQ ID NOs: 1, 4, 6, 7, 9, 10, 101, 102, 104, 106 and 107 .

[0093] Several methods exist in the art for the production of nucleic acid sequences and nucleic acid / DNA constructs of the invention. Procedures for identifying and isolating DNA clones are well known to those skilled in the art and are described, for example, by Sambrook et al., In Molecular Cloning-A Laboratory Manual (2nd Edition), Vol. 1-3, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 1989. Alternatively, the nucleic acid sequences of the invention can be produced by various in vitro amplification methods adapted to the present invention by the appropriate selection of specific or degenerate primers. Examples of protocols sufficient to guide technicians in the field by in vitro amplification methods, including polymerase chain reaction (PCR), ligase chain reaction (LCR), Qbeta-replicase amplification and other techniques mediated by RNA polymerase ( for example, NASBA) for, for example, the production of the homologous nucleic acids of the invention are described by Sambrook, mentioned above.

[0094] Alternatively, the nucleic acid constructs of the invention can be assembled from fragments produced from solid phase synthesis methods. Typically, fragments of up to approximately 100 bases are synthesized individually and then are linked enzymatically or chemically to produce a desired sequence, for example, a polynucleotide that encodes all or part of a transcription factor. For example, the synthesis

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35/362 chemistry using the phosphorus-amidite method is well known to a person skilled in the art. According to such methods, the oligonucleotides are synthesized, purified, rescanned to their complementary strands, ligated and then optionally cloned into suitable vectors. The invention also relates to vectors that comprise DNA constructs.

[0095] As mentioned, the sequences described above are hybrid black beech and Populus trichocarpa. As a technician in the field will understand, homologues of the described sequences can be isolated from other species, non-limiting examples of which include acacia, eucalyptus, hornbeam, beech, mahogany, walnut, oak, ash, bitter walnut, birch, chestnut, alder , maple, sycamore, gingko, palm, sweet gum, cypress, Douglas fir, fir, sequoia, hemlock, cedar, juniper, larch, redwood, firs and yew, apple, plum, pear, banana, orange, kiwi , lemon, cherry, vine, fig, cotton, bamboo, razor grass, red canopy and rubber plants. Useful homologues of the described sequences can also be isolated from hardwood vegetables of the Salicaceae family, for example, from the genus Salix and Populus. Members of these genera are known by their common names: willow, poplar and black beech.

[0096] Examples of other plants suitable for use according to any aspect of the invention described here include monocots, dicots, gymnosperms and algae, ferns and mosses. Transgenic higher plants are of particular interest, especially agricultural crops such as cereals and flowers, which have been modified to contain a heterologous nucleic acid construct as described above, including tobacco, cucurbits, carrots, cabbages, melons, peppers, vines , lettuce, strawberry, oilseed cabbage, beet, wheat, barley, corn, rice, sugar cane, soy, peas, sorghum, sunflower, tomato, potato, pepper, chrysanthemumPetition 870170074044, of 29/09/2017, p. 47/378

36/362 I fear, cloves, flaxseeds, hemp and rye.

[0097] In some preferred embodiments, the vegetable is a perennial plant, for example, a perennial woody plant. A perennial woody plant is a plant that has a life cycle longer than two years and involves a long youthful period, in which only vegetative growth occurs. This contrasts with herbaceous or annual vegetables such as Arabidopsis thaliana or Lycopersicon esculentum (tomato), which have a life cycle of one year.

[0098] In particular, the method according to the present invention can comprise the step of providing a nucleic acid construct, such as a recombinant DNA construct, which comprises a nucleotide sequence which, with respect to the particular sequences described, it comprises conservative variations that alter only one, or a few amino acids in the encoded polypeptide, can also be provided and used in accordance with the present invention. Thus, it is included in the scope of the invention to provide and use a recombinant DNA construct, which comprises a nucleotide sequence, which encodes a polypeptide comprising a conservatively substituted variant of a polypeptide of a).

[0099] Sequence changes that do not alter the amino acid sequence encoded by the polynucleotide are called silent substitutions. With the exception of the ATG and TGG codons, which encode methionine and tryptophan, respectively, any of the possible codons for the same amino acid can be replaced by several techniques available in the technique, such as site-directed mutagenesis. Thus, the present invention can also comprise a recombinant nucleic acid construct, wherein the nucleotide sequence comprises a silent substitution in a nucleotide sequence.

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37/362 [00100] In certain other embodiments of the invention, subsequences or fragments have at least 65% sequence identity with respect to a conserved domain of the nucleotide sequence as described above in item a) or d), as at least 70% identical, at least 75% identical, at least 80% identical, at least 85% identical, at least 87% identical, at least 90% identical, at least 95% identical, at least 97% identical, at least 98% identical, at least 99% identical, at least 99.5% identical to a conserved domain of a nucleotide sequence as described above in items a) or d).

[00101] Thus, methods exist to identify a similar or parallel or orthologous or homologous sequence to one or more polynucleotides as noted here, or one or more target polypeptides encoded by the polynucleotides or, otherwise, may include linking or associating a function genetics or a given plant phenotype with a sequence. In the methods, a sequence database is provided (locally, or over the internet or intranet) and a search is performed on this database using the relevant sequences here and associated genetic functions or plant phenotypes.

[00102] Approaches to obtaining a change in the level of a gene product [00103] This invention is used to increase the expression of certain genes; non-limiting examples of how this can be done are presented here. The construction of nucleic acids or construction of recombinant DNA as described above can be used for the identification of plants that have altered growth characteristics compared to the wild type. Such vegetables can, for example, be naturally occurring variants or plants that have been genetically modified to exhibit growth properties.

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38/362 ment amended. For such purposes, the nucleic acid construct or recombinant DNA construct according to the invention can be used as, for example, a probe in conventional hybridization assays or as a primer for specific amplification of nucleic acid fragments.

[00104] Although the main part of this invention is how an overload of gene products gives the desired effect, it also shows that changing the expression of genes presented here can be used to modify the desired properties, this is another way of looking for the data, and an effect of this view is that also decreasing the gene products in the plant is a way of modifying the desired trait. There are different ways to increase the levels of a gene product, these are described below in parallel with the means of suppressing the gene product.

[00105] One of the SEQ ID NO: 1 -13, 97 - 115 genes could also be used as a target for marker assisted reproduction, because changes in the regulatory sequences of the gene can yield changes in expression patterns and changes in coding sequences they can yield changes in the function of the gene, and it has been shown that the manipulation of these genes yields changes in the desired characteristics. This usually refers to the fact that the SEQ ID NO: 1-13, 97-115 genes can be used as candidate genes Brady and Provart 2007, and Varshney et al. 2005.

[00106] A particular way of using this invention is to measure the expression of one or more SEQ ID NO: 1-13, 97-115 genes using, for example, quantitative RT-PCR in natural populations and to select abnormally high expressions of the measured gene and using such vegetables as relatives in a breeding program, this could be repeated for each cycle of breeding. Methods for quantifying gene expression, including real-time PCR, are described by

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Sambrook et al.

[00107] The genes presented here can also be used in gene-based association studies, the result of such studies can then be used in marker assisted reproduction. Burke et al. 2007.

[00108] The overloading or overexpression of a gene can be achieved by placing the entire open reading frame of the gene behind a suitable promoter, which is described elsewhere, and usually by placing a 3 'polyadenylation signal sequence and terminator of the gene to be overexpressed.

[00109] Additionally, the nucleic acid construct or recombinant DNA construct according to the invention can be used for the purpose of gene replacement in order to modify the plant growth phenotype.

[00110] Suppression of expression of endogenous genes can be performed, for example, using a ribozyme. Ribozymes are RNA molecules that have highly specific endoribonuclease activity. The production and use of ribozymes is disclosed in US 4987071 and US 5543508. Although antisense techniques are discussed below, it should be mentioned that synthetic ribozyme sequences including antisense RNAs can be used to impart RNA dividing activity to antisense RNA, so that the endogenous mRNA molecules that hybridize to the antisense RNA are cleaved, which in turn leads to improved antisense inhibition of endogenous gene expression.

[00111] Vectors in which the RNA encoded by a relevant gene homologue is overexpressed can also be used to obtain the co-suppression of a corresponding endogenous gene, for example, in the manner indicated in US 5231020 by Jorgensen. Such cosuppression (also called sense suppression) does not require

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40/362 the entire sequence of the gene is introduced into plant cells, nor does it require that the sequence introduced be exactly identical to the endogenous sequence of interest. However, the suppression efficacy will be improved as the specificity of hybridization is increased, for example, as the introduced sequence is lengthened and / or as the sequence similarity between the introduced sequence and the endogenous transcription factor gene is increased. [00112] Vectors that express an untranslatable form of the gene, for example, sequences that comprise one or more stop codons, or nonsense mutation, can also be used to suppress the expression of an endogenous transcription factor, thereby reducing or eliminating its activity and modifying one or more characteristics. Methods for producing such constructions are described in the US patent. 5,583,021. In particular, such constructs can be made by introducing a premature stop codon into the gene. [00113] One way to carry out the insertion of the target DNA is by using retroviral DNA integration machinery as described in WO 2006078431. This technology is based on the possibility of changing the specificity of the retrovirus and retrotransposon integrase integration site by the operational coupling of integrase to a protein that binds to DNA (tethering protein). Modification of integrase is preferably carried out at the nucleic acid level, by modifying the wild type coding sequence of integrase by PCR. The integrase complex can thus be directed to a desired part or it can be directed away from an unwanted part of the genomic DNA, thus producing a desired integration site characteristic.

[00114] Another technology that can be used to alter and preferably, in this invention, increase gene expression is local lesions induced by screening in genomes, which is a non

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41/362 to alter genetic function in a controlled manner. This approach involves mutating a plant with an opponent, for example, ethyl methanesulfonate (without) and then locating individuals in which a particular desired gene has been modified. The technology is described, for example, by Slade and Knauf, 2005 and Henikoff, et al.

[00115] A method to abolish the expression of a gene is by insertion mutagenesis using the T-DNA of Agrobacterium tumefaciens. After the generation of the insertion mutants, the mutants can be monitored to identify those that contain the insertion in an appropriate gene. Vegetables containing a single transgenic insertion event in the desired gene can be crossed in order to generate homozygous plants for the mutation.

[00116] As a subject will become apparent to a technician, a characteristic of a vegetable can also be modified by the use of a cre-lox system. A plant genome can be modified to include a first and a second lox site which are then contacted with a Cre recombinase. As long as the lox sites are in the same orientation, the intervening DNA sequence between the two sites will be excised. If the lox sites are in opposite directions, the intervening sequence is reversed.

[00117] The polynucleotides and polypeptides of this invention can also be expressed in a plant in the absence of an expression cassette by manipulating the level of expression or activity of the endogenous gene by other means such as, for example, ectopic expression of a gene by tagging of T-DNA activation, Ichikawa et al. (1997); Kakimoto et al. (1996). This method involves transforming a plant with a gene marker that contains several transcription enhancers and, once the marker has been inserted into the genome, the expression of a sequence that encodes a gene

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42/362 flanking becomes unregulated. In another example, the transcription machinery in a plant can be modified to increase the levels of transcription of a polynucleotide of the invention (see, for example, PCT Order Publications WO 96/06166 and WO 98/53057, which describe the modification of the DNA binding specificity of zinc finger proteins by the change of amino acids in particular in the DNA binding motif).

Antisense suppression of expression [00118] However, the recombinant DNA construct, which comprises a nucleotide sequence as described above, is particularly useful for sense and antisense suppression of expression to, for example, to unregulate the expression of a particular gene, so to obtain a plant phenotype with increased growth. That is, the nucleotide sequence of the invention, or subsequences or antisense sequences thereof, can be used to block the expression of naturally occurring homologous nucleic acids. Several traditional sense and antisense technologies are known in the art, such as, for example, revealed by Lichtenstein and Nellen (1997). The aim of the antisense approach is to use a sequence complementary to the target gene to block its expression and create a mutant cell line or organism in which the level of the chosen protein is selectively reduced or abolished.

[00119] For more elaborate descriptions of the antisense regulation of gene expression as applied to plant cells, see US 5107065, the content of which is incorporated herein by reference in its entirety.

Interference RNA [00120] The gene silencing that is induced by double-stranded RNA is often called interference RNA or RNAi. Interference RNA is a molecular mechanism in which fragments

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43/362 double-stranded ribonucleic acid (dsRNA) interferes with the expression of a particular gene that shares a homologous sequence with the dsRNA. The process that is mediated by the same cellular machinery as microRNA processes, known as RNA-induced silencing complex (RISC). The process is initiated by the Dicer ribonuclease protein, which binds and cleaves exogenous double-stranded RNA molecules to produce 20 to 25 base pair double-stranded fragments with a few unpaired protruding bases at each end. The short fragments of double strand produced by Dicer, called small interfering RNAs (siRNAs), are separated and integrated into the active RISC complex. If a portion of the RNA transcript is targeted by an RNAi molecule or construct, the entire transcript is unregulated.

[00121] For more elaborate descriptions of gene suppression by RNAi in plants by transcription of a dsRNA, see US 6506559, US 2002/0168707 and WO 98/53083, WO 99/53050 and WO 99/61631, all of which are incorporated herein in full as a reference. Construction of vectors [00122] In general, technicians in the field are quite capable of constructing the vectors of the present invention and designing protocols for the expression of recombinant genes. For details on the general protocols for the preparation of vectors see: Molecular Cloning: a Laboratory Manual: 2nd edition, Sambrook et al, 1989, Cold Spring Harbor Laboratory Press.. The promoter used for the gene can influence the level, period, tissue, specificity or level of induction of overexpression.

[00123] In general, the overexpression of a gene can be performed using a recombinant DNA construct that has a promoter operationally linked to a DNA element that comprises a sense element of a genomic DNA or cDNA segment

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For example, the segment of the gene should contain enough of the open reading frame to produce a functional protein and, preferably, the entire open reading frame.

[00124] In pertinent embodiments of the invention, the nucleic acid construct or the recombinant DNA construct also comprises a tissue-specific, inducible or constitutive promoter operably linked to such a nucleotide sequence.

[00125] In a currently preferred embodiment of the present invention, the nucleic acid construct or the recombinant DNA construct, comprises the sequence SEQ ID NO: 96, the vector.

[00126] The currently preferred nucleic acid construct for overexpression is a vector called pK2GW7. The vector is described in: Gateway vectors for Agrobacterium-mediated plants transformation, Karimi, 2002.

Transformation of plant cells [00127] According to the present invention, the method comprises the additional step of transforming regenerable cells of a plant with such a nucleic acid construct or recombinant DNA construct and regenerating a transgenic plant from such transformed cell. When introducing the above DNA construct or vector into a plant cell, certain considerations must be taken into account, as known to a person skilled in the art. The nucleic acid to be inserted must be mounted within a construction that contains effective regulatory elements that will direct transcription, as described above. A method must be available to transport the construction into the cell. Once the construction is inside the cell, integration into the endogenous chromosomal material will occur or not.

[00128] Transformation techniques, well known to those skilled in the art, can be used to introduce constructions and vetoes

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45/362 resins of DNA in plant cells to produce transgenic plants, transgenic trees in particular, with improved plant growth.

[00129] A person skilled in the art will understand that a wide variety of host cells can be used as a container for the constructs and DNA vectors according to the invention. Non-limiting examples of host cells include cells in embryonic tissue, callus tissues of type I, II and III, hypocotyls, meristems, root tissues, tissues for expression in phloem.

[00130] As listed above, transformation by Agrobacterium is a method widely used by technicians in the field for the transformation of tree species, in particular hardwood species such as poplar. The production of fertile and stable transgenic plants is now routine in the art. Other methods, such as particle bombardment or microprojectiles, electroporation, microinjection, direct DNA absorption, liposome-mediated DNA absorption or the vortex method can be used where Agrobacterium transformation is inefficient or ineffective as in, for example, some species of gymnosperms.

[00131] Alternatively, a combination of different techniques can be employed to improve the efficiency of the transformation process, for example, bombardment with microparticles coated with Agrobacterium or bombardment with microprojectiles to induce wounds, followed by coculture with Agrobacterium.

[00132] It should be understood that the particular choice of transformation technology will be determined by its effectiveness in transforming certain plant species, as well as by the experience and preference of the person who is practicing the invention with a particular methodology of choice. It will be clear to technicians in the field that the particular choice of a transformation system for introducing

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46/362 zirring nucleic acids within plant cells is not essential or limiting to the invention, nor is it the choice of technique for plant regeneration.

[00133] After transformation, transgenic plants are preferably selected using a selectable marker incorporated into the transformation vector. Typically, such a marker will give herbicidal or antibiotic resistance to the transformed plant and the selection of the transformants can be carried out by exposing the plants to the appropriate concentrations of the antibiotic or herbicide. A new selection marker, which uses the D form of amino acids and based on the fact that plants can only tolerate the L form, offers a fast, efficient and environmentally friendly selection system. An interesting feature of this selection system is that it allows both selection and counter-selection.

[00134] Next, a plant can be regenerated from, for example, single cells, callus tissue or leaf discs, as is standard in the art. Almost any plant can be entirely regenerated from the plant's cells, tissues or organs. Available techniques are examined by Vasil etal., 1984.

[00135] After the transformed plants are selected and grown to maturity, those plants that show an increase in the growth phenotype are identified. In addition, to confirm that the phenotype is due to changes in the levels of expression or activity of the polypeptide or polynucleotide disclosed herein, mRNA expression is analyzed with Northern blots, RT-PCR or microarrays or protein expression using immunoblots or Western blots or displacement with gel.

Plant species [00136] According to the invention, the present method produces a transgenic plant that has increased growth if compared

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47/362 to its wild type from which it is derived. In a modality of the present method, the transgenic plant is a perennial plant, that is, a plant that lives for more than two years. In a specific embodiment, the perennial is a woody plant that can be defined as a vascular plant that has a stem (or more than one stem) that is lignified at a high level.

[00137] In a preferred embodiment, the woody plant is a hardwood plant, that is, angiosperm or broad-leaved trees, which can be selected from the group consisting of acacia, eucalyptus, hornbeam, beech, mahogany , walnut, oak, ash, bitter walnut, birch, chestnut, alder, maple, sycamore, gingko, palm, sweet gum. Hardwood plants of the Salicaceae family such as willow, poplar and black beech, including variants of these, are of particular interest, as these two groups include fast-growing species of tree or woody shrub that are grown specifically to provide firewood and biofuel for heating. Cellulosic grasses used for bioenergy like razor grass and red birdseed are also of interest.

[00138] In other modalities, the woody plant is a conifer that can be selected from the group consisting of cypress, Douglas fir, fir, sequoia, hemlock, cedar, juniper, larch, brazilwood, fir and yew.

[00139] In useful modalities, the woody plant is a fruitful plant that can be selected from the group consisting of apple, plum, pear, banana, orange, kiwi, lemon, cherry, vine, and fig.

[00140] Other woody plants that can be useful in the present method can also be selected from the group consisting of cotton, bamboo and rubber plants.

DNA construction [00141] According to a second main aspect of the invention,

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48/362 a DNA construct, such as a recombinant DNA construct, is provided comprising at least one sequence as described above. In particular, the recombinant DNA construct can comprise a nucleotide sequence selected from the group consisting of:

a) a nucleotide sequence comprising a sequence selected from SEQ ID NO: 1-13, 97-115;

b) a nucleotide sequence complementary to a nucleotide sequence of a);

c) a subsequence or fragment of a nucleotide sequence of a) or b);

d) a nucleic acid sequence that is at least 60% identical to any of the sequences in a), b) and c); and

e) a nucleotide sequence that hybridizes under stringent conditions to a nucleotide sequence of a), b) or c).

[00142] In selected embodiments of the invention, the nucleic acid sequence in d) is at least 65% identical to any of the sequences in a), b) and c), as well as at least 70% identical, at least 75% identical at least 80% identical, at least 85% identical, at least 87% identical, at least 90% identical, at least 95% identical, at least 97% identical, at least 98% identical, at least 99% identical, at least minus 99.5% identical to any of the sequences in a), b) and c).

[00143] Also, according to the above discussion, the nucleotide sequence encodes a polypeptide comprising a conservatively substituted variant of a polypeptide from (a). In addition, the nucleotide sequence comprises a silent replacement in the nucleotide sequence.

[00144] In additional modalities regarding this aspect of

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49/362 invention, subsequences or fragments have at least 65% sequence identity to a conserved domain of a nucleotide sequence as described in item a), as at least 70% identical, at least 75% identical, at least at least 80% identical, at least 85% identical, at least 87% identical, at least 90% identical, at least 95% identical, at least 97% identical, at least 98% identical, at least 99% identical, or at least 99.5% identical to a conserved domain of a nucleotide sequence as described in a).

[00145] In other embodiments and according to the above description, the recombinant DNA construct also comprises a constitutive, inducible or tissue-specific promoter operably linked to such a nucleotide sequence. In particular, the recombinant DNA construct can also comprise a strong constitutive promoter in front of a transcribed cassette consisting of the entire open reading frame of the gene followed by a terminator sequence. Such a cassette may comprise a nucleotide sequence as defined in cassette 7 and on page 21 and in the paragraph between pages 21 and 22.

[00146] In the presently exemplified embodiments of the invention, the recombinant DNA construct comprises the sequence of SEQ ID NO: 96.

Transgenic plants [00147] A third aspect of the invention provides a transgenic plant that comprises a recombinant polynucleotide (DNA construct) comprising a nucleotide sequence capable of altering in the plant the level of a gene product from at least one of the SEQ ID 1 genes - 13, 97 - 115. Given the increased growth when comparing such a group of transgenic plants grown for 8 weeks in a greenhouse under a photoperiod of 18 hours, temperature

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22 ° C / 15 ° C (day / night) and weekly fertilization with N 84 g / L, PI 2 g / L, K 56 g / L with a group of wild type plants under identical conditions.

[00148] According to a particular embodiment of the invention, the level of a gene product of at least one gene comprising a nucleotide sequence selected from the group consisting of:

a) a nucleotide sequence of SEQ ID NO: 1-13, 97-115;

b) a nucleotide sequence that is at least 60% identical to any of the sequences of SEQ ID NO 1 - 13, 97 - 115;

c) a subsequence or fragment of a nucleotide sequence from a) or b).

[00149] has been changed with respect to the level found in the corresponding corresponding wild type plant.

[00150] Still, according to another embodiment of the invention, the transgenic plant comprises a recombinant polynucleotide (DNA construction) comprising a nucleotide sequence selected from the group consisting of:

a) a nucleotide sequence of SEQ ID NO: 1-13, 97-115;

b) a nucleotide sequence complementary to a nucleotide sequence of a);

c) a subsequence or fragment of a nucleotide sequence from a) or b).

d) a nucleic acid sequence that is at least 60% identical to any of the sequences in a), b) and c); and

e) a nucleotide sequence that hybridizes under stringent conditions to a nucleotide sequence of a), b) or c).

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[00151] In additional embodiments of the invention, the nucleic acid sequence in c) or g) is at least 65% identical to any of the sequences in a), b), c), d) or e), and at least 70% identical, at least 75% identical, at least 80% identical, at least 85% identical, at least 87% identical, at least 90% identical, at least 95% identical, at least 97% identical, at least 98% identical, at least 99% identical, or at least 99.5% identical to any of the sequences in a), b), c), d) or e). The transgenic plant may also comprise a nucleotide sequence that encodes a polypeptide that comprises a conservatively substituted variant of a polypeptide of a) or b). The nucleotide sequence can comprise a silent substitution in a nucleotide sequence. In addition, subsequences or fragments can have at least 65% sequence identity to a conserved domain.

[00152] As mentioned above, a person skilled in the art will understand that several methods exist in the art for the production of nucleic acid sequences and polynucleotide constructions of the invention, such as cloning techniques, assembly of fragments generated by solid phase synthesis . Again, as a technician in the field will understand, homologues of the described sequences can be isolated from other species, non-limiting examples of these include acacia, eucalyptus, hornbeam, beech, mahogany, walnut, oak, ash, bitter walnut, birch, chestnut, alder, maple, sycamore, gingko, palm, sweet gum, cypress, Douglas fir, fir, sequoia, hemlock, cedar, juniper, larch, redwood, firs and yew, apple, plum, pear, banana, orange, kiwi, lemon, cherry, vine, fig, cotton, bamboo, razor grass, red birdseed and rubber plants. Useful counterparts of the described sequences can also be isolated from hardwood vegetables of the Salicace familyPetição 870170074044, of 29/09/2017, p. 63/378

52/362 ae, such as willow, poplar and black beech.

[00153] In particular, the transgenic plant according to the present invention may comprise a recombinant DNA construct that comprises a nucleotide sequence, which, in relation to the particular sequences described, comprises conservative variations altering only one, or a few amino acids in the encoded polypeptide can also be provided and used in accordance with the present invention. Thus, it is within the scope of the invention to provide a transgenic plant comprising a recombinant DNA construct comprising a nucleotide sequence which encodes a polypeptide which comprises a conservatively substituted variant of a polypeptide of a) or d).

[00154] Thus, the present invention can also provide a recombinant DNA construct in which the nucleotide sequence comprises a silent substitution in a nucleotide sequence, i.e., the recombinant DNA construct can comprise a change in the sequence that does not change the amino acid sequence encoded by the polynucleotide.

[00155] In certain other embodiments of the invention, subsequences or fragments have at least 65% sequence identity with respect to a conserved domain of a nucleotide sequence as described above in a) or d), such as at least 70 % identical, at least 75% identical, at least 80% identical, at least 85% identical, at least 87% identical, at least 90% identical, at least 95% identical, at least 97% identical, at least 98% identical , at least 99% identical, or at least 99.5% identical to a conserved domain of a nucleotide sequence as described above in a) or d).

[00156] In the particular modalities by which the present invention is exemplified, the subsequences or fragments in c) comprPetição 870170074044, of 29/09/2017, p. 64/378

53/362 end with the sequences of SEQ ID Nos: 18-34.

[00157] In other embodiments, the transgenic plant provided in accordance with the present invention comprises a recombinant polynucleotide construct that also comprises a constitutive, inducible or tissue-specific promoter operably linked to such a nucleotide sequence.

[00158] Still, in other embodiments, the construction of recombinant polynucleotides also comprises a strong constitutive promoter in front of a transcribed cassette. The cassette may comprise a nucleotide sequence, in which the modulated expression is effected by introducing a genetic modification, preferably at the site of a gene encoding a polypeptide comprising SEQ ID NO: 1 - 13, 97 - 115 or a homolog of such a polypeptide followed by a functional plant intron followed by a nucleotide sequence encoding a polypeptide comprising a conservatively substituted variant of a polypeptide of a) or d) in reverse orientation.

[00159] In a presently preferred embodiment of the invention, the transgenic plant according to the invention comprises a recombinant DNA construct comprising the sequence of SEQ ID NO: 96

Plant species [00160] According to the present invention, the transgenic plant may be a perennial plant that is preferably a woody plant or a woody species. In a useful modality, a woody plant is a hardwood plant, which can be selected from the group consisting of acacia, eucalyptus, hornbeam, beech, mahogany, walnut, oak, ash, bitter walnut, willow, birch, chestnut , alder, maple, sycamore, gingko, palm, and sweet gum. Hardwood plants such as those of the Salicaceae family such as willow, poplar

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54/362 and black beech, including variants of these, are of particular interest, as these two groups include fast-growing species of tree or woody shrub that are grown specifically to provide firewood and biofuel for heating.

[00161] In other modalities, the woody plant is a conifer that can be selected from the group consisting of cypress, Douglas fir, fir, sequoia, hemlock, cedar, juniper, larch, brazilwood, fir and yew.

[00162] In useful modalities, the woody plant is a fruitful plant that can be selected from the group consisting of apple, plum, pear, banana, orange, kiwi, lemon, cherry, vine, and fig.

[00163] Other woody plants that can be useful in the present method can also be selected from the group consisting of cotton, bamboo and rubber plants.

[00164] The present invention extends to any plant cell of the transgenic plants obtained above by the methods described herein, and to all plant parts, including plant parts, seeds and propagules thereof, including parts that can be harvested, and the explant of the plant or plant tissue. The present invention also encompasses a plant, a part thereof, a plant cell or a plant progeny which comprises the construction of DNA according to the invention. The present invention also extends to encompass the progeny of a cell, tissue, organ or whole plant primarily transformed or transfected that was produced by any of the above methods, the only requirement being that the progeny require the same genotypic characteristics and / or phenotypic than those produced in the relative by methods according to the invention.

[00165] It should be noted that the modalities and characteristics described in the context of one aspect of the present invention also

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55/362 apply to other aspects of the invention. Thus, the definitions of a modality are mutatis mutandis related to all other modalities that comprise or are related to that modality. When, for example, definitions are made with respect to DNA construction or sequences, such definitions also relate to, for example, methods for the production of plants, vectors, plant cells, biomass and firewood that comprise the construction of DNA and vice versa. A DNA construct described in relation to a plant also refers to all other modalities.

[00166] All references to patents and non-patents cited in this specification are hereby incorporated by reference, in full.

[00167] The invention will now be described in more detail in the following non-limiting examples

Examples

Introduction [00168] In order to find and elucidate the function of the genes involved in growth, an extensive genetic mining program was carried out, resulting in the identification of genes useful in increasing growth and which have industrial application.

Materials and methods

Selection of genes [00169] The first stage of this genetic mining program was to select a number of genes from a large pool of genes to restrict the genes to be tested for their functions.

[00170] We decided to test transcription factors. The reason for selecting transcription factors for analysis is because they have long been known to regulate part of many, if not most, of living organism processes.

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56/362 [00171] Although the selection of genes, which will have their functions analyzed, is an important part of discovering genes with interesting functions for forest biotechnology in an economical and effective way, it is testing the function of the gene of the genes selected which is the crucial step to find its use in industrial applications. [00172] The genes of the transcription factors were identified by BLAST analysis of the genes present in Populus DB, Sterky et al. 2004, regarding plant genes noted as transcription factors present in databases such as http://plntfdb.bio.unipotsdam.de/v2.0, described by Riano-Pachon et al. 2007 and http://www.ncbi.nlm.nih.gov/. In some examples, the genes were also selected based on having a differential expression pattern during the formation of the wood (the genes that correspond to the TFSTT 019, 035, 047 and 051 constructions).

Cloning of selected genes [00173] The corresponding gene models for the selected genes were extracted from data derived from the genome sequencing of Populus trichocarpa, Tuskan et al. 2006 using the BLAST analysis. The gene models were compared, and in some instances corrected based on published information for homologous genes in Arabidopsis thaliana and other plant species. This was done using databases such as http://www.ncbi.nlm.nih.gov/ and http://www.arabidopsis.org/. Then, the selected genes were cloned into an overexpression vector under the control of the CaMV 35S promoter. For the isolation of cDNA, the total RNA was isolated from the stem, bark tissue and leaves sampled from clones of T89 hybrid black beech plants and reverse transcribed to cDNA using Superscript III First Strand Synthesis System (Invitrogen). The cDNA was then amplified by PCR with specific gene forward and reverse primers, using the polymerase

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High fidelity DNA Phusion (Finnzymes). The PCR primers were selected as follows, the 5 'primer was placed on the start codon and the 3' reverse primer was placed on 3 'of the transcription stop site. The amplified cDNAs were inserted into a Gateway vector input pENTR / D-TOPO (Invitrogen), followed by gene transfer in the expression vector pK2GW7 (SEQ ID NO: 96) using the LR Gateway recombination reaction (Invitrogen). The cloned genes were sequenced for control and compared to the selected genes using standard techniques before subcloning in the plant vector pK2GW7.

[00174] The gene subsequences, polypeptide sequences and PCR primers for the genes presented are listed in Tables A to C.

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Table A - PCR cloning primers

Construction Direct cloning initiator Reverse cloning initiator TF0002 SEQ ID NO: 27 SEQ ID NO: 40 TF0052 SEQ ID NO: 28 SEQ ID NO: 41 TF0065 SEQ ID NO: 29 SEQ ID NO: 42 TF0076 SEQ ID NO: 30 SEQ ID NO: 43 TF0089 SEQ ID NO: 31 SEQ ID NO: 44 TF0109 SEQ ID NO: 32 SEQ ID NO: 45 TF0132 SEQ ID NO: 33 SEQ ID NO: 46 TFSTT051 SEQ ID NO: 34 SEQ ID NO: 47 TF0013 SEQ ID NO: 35 SEQ ID NO: 48 TF0097 SEQ ID NO: 36 SEQ ID NO: 49 TFSTT019 SEQ ID NO: 37 SEQ ID NO: 50 TFSTT035 SEQ ID NO: 38 SEQ ID NO: 51 TFSTT047 SEQ ID NO: 39 SEQ ID NO: 52

Construction Direct cloning initiator Reverse cloning initiator TF0003 SEQ ID NO: 135 SEQ ID NO: 154 TF0011 SEQ ID NO: 136 SEQ ID NO: 155 TF0045 SEQ ID NO: 137 SEQ ID NO: 156 TF0058 SEQ ID NO: 138 SEQ ID NO: 157 TF0096 SEQ ID NO: 139 SEQ ID NO: 158 TF0104 SEQ ID NO: 140 SEQ ID NO: 159 TF0116 SEQ ID NO: 141 SEQ ID NO: 160 TF0146 SEQ ID NO: 142 SEQ ID NO: 161 TF0173 SEQ ID NO: 143 SEQ ID NO: 162 TF0247 SEQ ID NO: 144 SEQ ID NO: 163 TF0405 SEQ ID NO: 145 SEQ ID NO: 164 TFSTT001 SEQ ID NO: 146 SEQ ID NO: 165 TFSTT004 SEQ ID NO: 147 SEQ ID NO: 166 TFSTT013 SEQ ID NO: 148 SEQ ID NO: 167 TFSTT016 SEQ ID NO: 149 SEQ ID NO: 168 TFSTT017 SEQ ID NO: 150 SEQ ID NO: 169 TFSTT036 SEQ ID NO: 151 SEQ ID NO: 170 TFSTT038 SEQ ID NO: 152 SEQ ID NO: 171 TFSTT045 SEQ ID NO: 153 SEQ ID NO: 172

Binary target vector: pK2GW7

SEQ ID NO: 96

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Table B - Polypeptide and gene cDNA sequences overexpressed

Construction Genetic model for the cDNA sequence of the overexpressed gene Predicted overexpressed protein sequence Species TF0002 SEQ ID NO: 1 SEQ ID NO: 14 Populus tríchocarpa TF0052 SEQ ID NO: 2 SEQ ID NO: 15 Populus tríchocarpa TF0065 SEQ ID NO: 3 SEQ ID NO: 16 Populus tríchocarpa TF0076 SEQ ID NO: 4 SEQ ID NO: 17 Populus tríchocarpa TF0089 SEQ ID NO: 5 SEQ ID NO: 18 Populus tríchocarpa TF0109 SEQ ID NO: 6 SEQ ID NO: 19 Populus tríchocarpa TF0132 SEQ ID NO: 7 SEQ ID NO: 20 Populus tríchocarpa TFSTT051 SEQ ID NO: 8 SEQ ID NO: 21 Populus tríchocarpa TF0013 SEQ ID NO: 9 SEQ ID NO: 22 Populus tríchocarpa TF0097 SEQ ID NO: 10 SEQ ID NO: 23 Populus tríchocarpa TFSTT019 SEQ ID NO: 11 SEQ ID NO: 24 Populus tríchocarpa TFSTT035 SEQ ID NO: 12 SEQ ID NO: 25 Populus tríchocarpa TFSTT047 SEQ ID NO: 13 SEQ ID NO: 26 Populus tríchocarpa

TF0003 SEQ ID NO: 97 SEQ ID NO 116 Populus tríchocarpa TF0011 SEQ ID NO: 98 SEQ ID NO 117 Populus tríchocarpa TF0045 SEQ ID NO: 99 SEQ ID NO 118 Populus tríchocarpa TF0058 SEQ ID NO 100 SEQ ID NO 119 Populus tríchocarpa TF0096 SEQ ID NO 101 SEQ ID NO 120 Populus tríchocarpa TF0104 SEQ ID NO 102 SEQ ID NO 121 Populus tríchocarpa TF0116 SEQ ID NO 103 SEQ ID NO 122 Populus tríchocarpa TF0146 SEQ ID NO 104 SEQ ID NO 123 Populus tríchocarpa TF0173 SEQ ID NO 105 SEQ ID NO 124 Populus tríchocarpa TF0247 SEQ ID NO 106 SEQ ID NO 125 Populus tríchocarpa TF0405 SEQ ID NO 107 SEQ ID NO 126 Populus tríchocarpa TFSTT001 SEQ ID NO 108 SEQ ID NO 127 Populus tríchocarpa TFSTT004 SEQ ID NO 109 SEQ ID NO 128 Populus tríchocarpa TFSTT013 SEQ ID NO 110 SEQ ID NO 129 Populus tríchocarpa TFSTT016 SEQ ID NO 111 SEQ ID NO 130 Populus tríchocarpa TFSTT017 SEQ ID NO 112 SEQ ID NO 131 Populus tríchocarpa TFSTT036 SEQ ID NO 113 SEQ ID NO 132 Populus tríchocarpa TFSTT038 SEQ ID NO 114 SEQ ID NO 133 Populus tríchocarpa TFSTT045 SEQ ID NO 115 SEQ ID NO 134 Populus tríchocarpa

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Table C - Cloned cDNA control sequences

Construction Complete control sequence of the cloned cDNA 5 'control sequence of cloned cDNA 3 'cloned cDNA control sequence Species TF0002 SEQ ID NO: 73 Populus tremula x tremuloides TF0052 SEQ ID NO: 74 Populus tremula x tremuloides TF0065 SEQ ID NO: 76 SEQ ID NO: 86 Populus tremula x tremuloides TF0076 SEQ ID NO: 77 SEQ ID NO: 87 Populus tremula x tremuloides TF0089 SEQ ID NO: 78 SEQ ID NO: 88 Populus tremula x tremuloides TF0109 SEQ ID NO: 75 Populus tremula x tremuloides TF0132 SEQ ID NO: 79 SEQ ID NO: 89 Populus tremula x tremuloides TFSTT051 SEQ ID NO: 80 SEQ ID NO: 90 Populus tremula x tremuloides TF0013 SEQ ID NO: 81 SEQ ID NO: 91 Populus tremula x tremuloides TF0097 SEQ ID NO: 82 SEQ ID NO: 92 Populus tremula x tremuloides TFSTT019 SEQ ID NO: 83 SEQ ID NO: 93 Populus tremula x tremuloides TFSTT035 SEQ ID NO: 84 SEQ ID NO: 94 Populus tremula x tremuloides TFSTT047 SEQ ID NO: 85 SEQ ID NO: 95 Populus tremula x tremuloides

TF0003 SEQ ID NO: 173 Populus tremula x tremuloides TF0011 SEQ ID NO: 174 Populus tremula x tremuloides TF0045 SEQ ID NO: 180 SEQ ID NO: 192 Populus tremula x tremuloides TF0058 SEQ ID NO: 175 Populus tremula x tremuloides TF0096 SEQ ID NO: 181 SEQ ID NO: 193 Populus tremula x tremuloides TF0104 SEQ ID NO: 182 SEQ ID NO: 194 Populus tremula x tremuloides TF0116 SEQ ID NO: 183 SEQ ID NO: 195 Populus tremula x tremuloides

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TF0146 SEQ ID NO: 184 SEQ ID NO: 196 Populus tremula x tremuloides TF0173 SEQ ID NO: 176 Populus tremula x tremuloides TF0247 SEQ ID NO: 177 Populus tremula x tremuloides TF0405 SEQ ID NO: 185 SEQ ID NO: 197 Populus tremula x tremuloides TFSTT001 SEQ ID NO: 186 SEQ ID NO: 198 Populus tremula x tremuloides TFSTT004 SEQ ID NO: 187 SEQ ID NO: 199 Populus tremula x tremuloides TFSTT013 SEQ ID NO: 178 Populus tremula x tremuloides TFSTT016 SEQ ID NO: 188 SEQ ID NO: 200 Populus tremula x tremuloides TFSTT017 SEQ ID NO: 189 SEQ ID NO: 201 Populus tremula x tremuloides TFSTT036 SEQ ID NO: 190 SEQ ID NO: 202 Populus tremula x tremuloides TFSTT038 SEQ ID NO: 191 SEQ ID NO: 203 Populus tremula x tremuloides TFSTT045 SEQ ID NO: 179 Populus tremula x tremuloides

Plant transformation [00175] CaMV 35S: Overexpressed DNA constructions were transformed into Agrobacterium and then into hybrid black beech, in which a T89 clone of Populus tremula L. x P. tremuloides Minch, hereinafter called poplar, it was transformed and regenerated essentially as described by Nilsson et al. (1992). Approximately 3 to 8 independent strains were generated for each construction. One such group of transgenic trees produced using such a construction is hereinafter referred to as construction groups, for example, different transgenic trees emanating from a construction. Each transgenic strain within each construction group, for example, TF0555-2B, TF0555-3A and so on, are different transformation events and thus more likely to have the recombinant DNA inserted at different locations in the plant genome. This causes different strains within a group

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62/362 building blocks are partially different. For example, it is known that different transformation events will produce vegetables with different levels of genetic overexpression. Construction groups called, for example, TF0555RP with individuals such as TF055RP-2B are the same as those without the RP part. RP means that this is a replanting of the same construction group as that without the RP part. RP2 means the second replanting, RP3 means the third replanting and so on.

Plant growth [00176] The transgenic poplar strains were grown together with their wild type control trees (wt), in a greenhouse under a 18h photoperiod and at a temperature of 22 ° C / 15 ° C (day / night). The plants were fertilized weekly with Weibulls Rika S NPK 7-1-5 diluted 1 to 100 (final concentrations NO ₃ , 55 g / L; NH ₄ , 29 g / L; P, 12 g / L; K, 56 g / L; Mg 7.2 g / L; S, 7.2 g / L; B, 0.18 g / L; Cu, 0.02 g / L; Fe, 0.84 g / L; Mn, 0, 42 g / L; Mo, 0.03 g / L; Zn, 0.13 g / L). The plants were grown for 8 or 9 weeks before harvest. During this time, its height and diameter were measured once or twice a week. In a growth group, an amount of wild-type trees (typically between 35 and 45 trees) and an amount of transgenic trees comprising several construction groups (typically 6-20 construction groups) were grown in parallel in the greenhouse under the same conditions as above. All comparisons between wild type trees and construction groups are made within each group.

Sampling [00177] Two main types of crops and sampling were performed. A general type was designed for, for example, chemical analysis, analysis of wood morphology, analysis of gene expression, analysis of wood density and metabolomic analysis. The second

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63/362 type was designed for dry weight measurements of bark, leaves and roots.

Selection of construction groups [00178] In the first round of growth for each group of trees with a specific overexpressed gene, that is, a construction group, a number of the following analyzes were performed: measures of growth and, in many cases, of wood density. These data were analyzed in order to highlight the construction groups that showed a phenotypic variation, for example, increased growth compared to wild type control trees.

Replanting and regrowth [00179] Based on growth data in the first round of greenhouse growth, groups of trees with a specific overexpressed gene, that is, a construction group, were selected, replanted and regrowth under the same conditions as in the first round growth. Transgenic poplar strains selected from each construction group were re-grown in triplicates. The number of the replanting round and the numbers of individual replicates of the plant lineage have been added to the names of the constriction groups to keep them unique, for example, TF0555rp1-2B-1, TF0555rp1-2B-2, TF0555rp1-2B-3 , where rp1 means the first replanting round of the construction group TF0555 strain 2B and 1, -2, -3 denote the individual replica of the plant strain. Similarly, rp2 means the second round of replanting. In cases where strains of construction groups not included in the first round of greenhouse growth are planted, a suffix (2nd) is added to the name of the construction group to illustrate the fact.

[00180] Based on the growth data, several factors of growth rates and analyzes were carried out and calculated manually.

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64/362 to select the construction groups and, thus, the genes in which it is possible to use them to alter growth characteristics. The selection criteria and methods are described below.

Example 1

Growth analysis

Maximum height growth rate [00181] A measurement of the height growth rate (here called maximum height growth rate) was defined as the curve of a linear function fitted over four consecutive data points. A value for the height growth rate was calculated for data points 1 to 4, data points 2 to 5, etc. in steps, see Figure 1 for an example. A maximum height growth rate, defined as the maximum value produced by the stepwise linear regression analysis for each plant, was computed. The primary data for the values of the maximum height growth rates of individual transformers in a construction group were checked so that they were not based on bad values. From Figure 1, showing an example of a height growth curve, it can be seen that the height growth rate increases during the first part of the growth, so the plants reach their maximum height growth rate and then the growth rate decreases as the plant gets bigger. As these phases have different periods in different plants and there is some noise added to the measurement of the plants described above, the maximum height growth using the rate method is very useful in calculating the maximum growth speed in these conditions for different individual trees.

Diameter growth rate [00182] Under the growth conditions defined above, the stem width exhibits a comparatively linear increase over time

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65/362 po described by the formula d (t) = c * t + d ₀ , where d ₀ is the initial width and c is the rate of growth of the diameter (curve). Linear regression was used in the diameter data to estimate a diameter growth rate.

Final diameter and height [00183] Final diameters and heights were also used to select construction groups with altered growth characteristics. These values take into account both the growth capacity of the trees and the ability of the trees to start growing when transferred from the tissue culture to the soil and placed in a greenhouse.

Selection parameters [00184] The construction groups that showed a significant or pronounced increase compared to the wild type population in the growth parameters mentioned above, ie growth rate in diameter, maximum growth rate in height, final height and diameter Finally, they were identified as construction groups that have altered growth properties. Thus, the corresponding genes can be used to alter these properties. The selection criteria are revealed below. Two levels of different selection criteria were used, a basic level and one for constructions generating growth phenotypes of extra interest.

Growth difference selection criteria [00185] Table 1.2 lists the abbreviations used for the different growth parameters, when used to describe the phenotypes of the construction groups.

Table 1.2- Abbreviations used for the different phenotypes.

AFH Average final height of the wild type population and each construction group population.

AFD Average final diameter of the wild type population and each

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66/362 construction group population.

AMHGR Average maximum rate of growth in height of the wild type population and each population of the construction group.

ADGR Average growth rate of the diameter of the wild type population and each population of the construction group.

MFH Maximum final height of the wild type population and each construction group population.

MFD Maximum final diameter of the wild type population and each population of the construction group.

MMHGR Maximum maximum growth rate in height of the wild type population and each population of the construction group.

MDC Maximum growth rate in diameter of the wild type population and each construction group population.

[00186] The growth difference selection criteria are as follows:

1. If AFH, MFH, AMHGR and MMHGR of the construction group are at least 5% (or 8% on a more stringent second level) greater than the AFH, MFH, AMHGR and MMHGR of the corresponding wild type group, or

2. If the construction group's AFD, MFD, ADGR and MDC are at least 5% (or 8% on a more stringent second level) greater than the corresponding wild-type group's AFD, MFD, ADGR and MDC, or

3. If the building group's AFH, AFD, AMHGR or ADGR are at least 5% (or 8% on a more stringent second level) greater than the corresponding wild type group's AFH, AFD, AMHGR or ADGR, or

4. If MFH, MFD, MMHGR or MDC of the construction group

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67/362 are at least 5% (or 8% on a more stringent second level) greater than the corresponding wild type group's MFH, MFD, MMHGR or MDC [00187] Running a large-scale functional genome program produces a certain amount variation and uncertainty in the data produced. In this configuration, the variation originates from sources such as: the different strains within a construction group have different levels of overexpression, which results in one of all the strains tested within a construction group being able to present the phenotype; the variation in growth that occurs during the experimental procedure due to small variations in plant status when transferring plants from tissue culture to the greenhouse, and variations based on different positions in the greenhouse at different time points during the growth cycle. These variations must be dealt with when analyzing the data. Based on this, we use two different increase limits, 5% and 18% for the selection of construction groups with increased growth. Selection criteria 1 and 2 use a 5% increase, however this increase must be present in all AFH, MFH, AMHGR and MMHGR phenotypes that correspond to height growth or in all AFD, MFD, ADGR and MDC phenotypes that correspond to to growth in diameter. In cases where the phenotype can only be seen in some or one of the plants and in only one of the types of phenotype, a higher increase of 18% was used to select positive construction groups so as not to select construction groups based on in random variations (selection criteria 3 and 4 select based on average values and maximum individual values respectively).

[00188] Construction groups that meet one or more of these criteria have been selected.

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Overexpression level analysis [00189] Real-time PCR was used to compare the expression levels of the construction genes of the recombinant overexpression construction group with the corresponding wild type group. The level of expression of the 26S S2 proteasome regulatory subunit was used as a reference for which the gene expression construct was normalized. The comparative CT method was used to calculate the relative levels of gene expression construction, in which the ratio between the reference gene expression construction is described by (1 + E _to iv ₀ ) ' ^CTalV0 / (1 ⁺ Ereference ) ^{'CTreferencia} wherein Eaivo and E _{a reference} are the efficiencies of construction and amplification by PCR of the reference gene , respectively , and CT i _V0 θ CT _{a reference} are the limit cycles as calculated for the construction and for gene amplification reference, respectively. The ratios between the construction and the expression levels of the reference gene were then normalized in relation to the average of the ratios of the wild type groups.

[00190] For the total extraction of RNA, samples of the stem (approximately 50 mg) were taken from plants grown in the greenhouse and instantly frozen in liquid nitrogen. The frozen samples were ground in a ball mill (Retsch MM301). The total RNA was extracted using the E-Z 96 plant RNA kit according to the manufacturer's recommendations (Omega Bio-Tek). CDNA synthesis was performed using the iScript cDNA synthesis kit according to the manufacturer's recommendations (Bio-Rad). RNA concentrations were measured and equal amounts were used for cDNA synthesis to ensure equal amounts of cDNA for PCR reactions. The cDNA was diluted 12.5x before real-time PCR.

[00191] Real-time PCR initiators are designed

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69/362 using Beacon Designer 6 (PREMIER Biosoft International), using the included tools to minimize the interference of the secondary model structure in the ringing sites of the initiators.

[00192] For real-time PCR, the cDNA model was mixed with the specific primers of the corresponding construction genes (SEQ ID NO: 53 to 61 and SEQ ID NO: 63 to 71), the specific primers of the reference genes internal (SEQ ID NO: 62 and 72) and SYBR Green Supermix (Bio-Rad). Real-time PCR reactions were performed on a MyiQ PCR thermocycler (Bio-Rad) and analyzed using the included iQ5 software. The reactions were carried out in triplicates, three times using the specific primers of the construction genes and three times using the specific primers of the reference genes for each sample, and the mean limit cycle for each triplicate was then used for the calculation relative levels of expression of the construction genes.

[00193] The 96-well plate was covered with microfilm and configured in the thermal cycler to begin the reaction cycle. For illustrative purposes, the reaction cycle may include the following steps: Initial denaturation at 95 ° C for 3 minutes and 30 seconds, followed by 40 rounds of amplification comprising the following steps, 95 ° C for 10 seconds, 55 ° C for 30 seconds and 72 ° C for 40 seconds.

Table 1.3 - Real-time PCR initiators

Construction Direct real-time PCR initiator Real-time reverse PCR initiator TF0002 SEQ ID NO: 53 SEQ ID NO: 63 TF0052 SEQ ID NO: 54 SEQ ID NO: 64 TF0065 SEQ ID NO: 55 SEQ ID NO: 65 TF0076 SEQ ID NO: 56 SEQ ID NO: 66 TF0089 SEQ ID NO: 57 SEQ ID NO: 67 TF0109 SEQ ID NO: 58 SEQ ID NO: 68 TFSTT051 SEQ ID NO: 59 SEQ ID NO: 69 TF0013 SEQ ID NO: 60 SEQ ID NO: 70 TF0097 SEQ ID NO: 61 SEQ ID NO: 71

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Construction Direct real-time PCR initiator Real-time reverse PCR initiator Real-time PCR reference gene: S2 subunit of the 26S proteasome regulator SEQ ID NO: 62 SEQ ID NO: 72

Results [00194] Raw growth data for the specified construction group and the corresponding wild type group are shown in Tables 1.4 to 1.16. The columns in the tables contain the height and diameter measurements of individuals from the specified construction group (called TF) and the corresponding wild type group (called T89). The measurement time, in the form of number of days in the greenhouse, is shown in the table headings.

[00195] Real-time PCR was used to confirm the overexpression of the constructions. Real-time PCR data tables contain the expression levels of the genes of the construction gene with respect to the expression of the reference gene. All ratios between the expression levels of the construct and the reference gene shown are normalized to the average of the ratios of the wild type group.

Construction Group TF002 [00196] This construction induces increased growth. The final height is 12% higher when comparing the average of the construction group with the control group of the wild type. The maximum rate of growth in height is 31% higher when comparing the average of the construction group with the control group of the wild type. Construction group TF0002 meets the most stringent level of growth difference selection criteria (3), as shown in table 1.4d.

[00197] Tables 1.4a and 1.4b contain growth data for the specified construction group and the wild-type color group

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71/362 respondent. The columns in the tables contain the height and diameter measurements of individuals from the specified construction group and the corresponding wild type group. The measurement time, in the form of number of days in the greenhouse, is shown in the table headings. Table 1.4a height growth data for TF0002

Height (cm)

Days in the greenhouse 18 22 25 29 32 39 46 50 53 TF0002-1B 12 18 23 35 49 82 115 134 150 TF0002-2A 15 21 27 40 55 87 122 142 157 TF0002-2B 9 12 15 23 36 64 95 113 129 TF0002-3A 17 22 29 42 57 92 129 150 166 TF0002-3B 16 21 24 39 52 83 117 136 154 TF0002-4B 14 19 25 38 52 85 121 141 159 T89-01 25 29 34 45 54 79 104 119 132 T89-02 28 33 41 54 64 83 109 125 136 T89-03 26 34 41 51 61 90 125 143 155 T89-04 29 36 42 58 72 98 127 146 159 T89-05 21 25 29 37 45 68 93 108 120 T89-06 25 31 39 49 60 83 109 125 137 T89-07 24 29 34 45 58 83 109 125 138 T89-08 24 32 41 53 67 94 121 138 150 T89-09 24 32 41 54 66 94 120 135 145 T89-10 20 26 30 44 54 79 108 123 135 T89-11 21 26 32 42 54 79 107 123 136 T89-12 25 32 40 55 66 97 125 140 151 T89-13 21 29 35 45 54 75 98 114 124 T89-14 25 31 38 50 60 85 111 125 135 T89-15 25 33 40 53 64 88 112 126 137 T89-16 24 28 33 42 49 68 89 102 112 T89-17 26 33 40 50 60 83 109 127 137 T89-18 24 29 37 47 55 80 108 123 133 T89-19 21 26 32 40 49 65 80 95 107 T89-20 26 32 40 52 65 98 127 146 159 T89-21 24 29 37 45 51 71 100 114 125 T89-22 25 30 39 48 63 83 110 125 139 T89-23 22 28 34 45 56 83 106 120 130 T89-24 24 30 38 49 60 82 106 124 138 T89-25 24 29 36 48 56 80 107 123 132 T89-26 26 35 41 52 68 87 110 123 136 T89-27 22 28 35 47 57 84 111 126 138 T89-28 24 29 36 46 58 87 110 123 136 T89-29 24 32 40 50 63 81 111 128 141 T89-30 26 31 37 46 53 72 95 107 118

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Days in the greenhouse 18 22 25 Height (cm) 29 32 39 46 50 53 T89-31 26 32 41 52 63 87 115 130 143 T89-32 28 33 41 52 63 88 116 130 142 T89-33 20 24 30 41 51 76 102 117 130 T89-34 25 30 39 51 63 90 117 130 138 T89-35 26 31 38 49 59 83 106 119 129 T89-36 25 33 40 52 59 73 91 104 116 T89-37 29 37 45 60 73 100 128 146 156

Table 1.4b - Diameter growth data for TF0002

Days in the greenhouse 29 32 Diameter (mm) 39 46 50 53 TF0002-1B 3.4 4.7 6.0 7.3 7.5 8.1 TF0002-2A 3.5 4.1 5.7 7.0 7.4 7.3 TF0002-2B 2.9 3.3 4.3 5.7 6.2 7.0 TF0002-3A 3.8 4.8 6.2 7.4 8.3 8.8 TF0002-3B 3.5 4.4 5.7 6.7 7.4 8.1 TF0002-4B 3.4 4.8 5.8 7.1 7.5 8.0 T89-01 3.8 4.6 5.9 7.2 7.6 8.1 T89-02 5.0 5.9 7.0 8.4 9.5 8.9 T89-03 4.9 6.4 7.3 8.8 8.8 9.6 T89-04 5.1 6.2 8.1 9.0 8.6 10.2 T89-05 3.8 4.2 5.0 6.1 5.9 6.7 T89-06 5.0 5.9 7.0 7.9 8.9 9.1 T89-07 4.0 5.0 6.4 7.5 8.3 9.3 T89-08 4.6 5.6 7.1 8.1 8.6 9.9 T89-09 5.1 6.2 8.0 9.3 9.7 10.3 T89-10 3.7 5.0 6.2 7.3 8.3 8.7 T89-11 3.8 4.5 6.0 7.4 8.2 8.7 T89-12 4.6 6.3 7.1 8.6 9.4 10.8 T89-13 5.0 5.2 6.0 6.8 7.2 7.7 T89-14 4.3 5.1 6.5 7.3 7.7 8.5 T89-15 5.6 6.0 7.4 8.4 8.9 9.6 T89-16 4.1 5.0 6.1 6.9 7.0 7.8 T89-17 5.5 6.2 7.2 7.9 8.7 9.0 T89-18 4.6 4.9 6.0 7.2 7.9 8.7 T89-19 4.0 5.2 6.5 7.7 7.0 8.4 T89-20 4.6 6.0 7.5 9.8 10.3 8.8 T89-21 4.5 4.7 5.6 6.8 5.8 7.8 T89-22 4.7 5.4 6.1 6.9 6.9 8.1 T89-23 4.0 5.0 6.2 7.7 8.5 9.0 T89-24 4.4 5.0 5.5 6.8 6.9 7.9 T89-25 4.4 5.5 6.1 7.8 8.2 7.3 T89-26 4.4 4.8 6.2 7.6 6.3 8.5 T89-27 4.4 5.1 5.8 7.1 9.0 7.2

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Days in the greenhouse 29 32 Diameter (mm) 39 46 50 53 T89-28 4.0 5.0 6.3 7.4 8.1 8.9 T89-29 4.7 6.0 7.1 8.3 7.8 9.3 T89-30 4.3 4.8 5.5 6.0 6.6 7.1 T89-31 4.6 5.5 6.4 7.8 9.0 9.3 T89-32 4.5 5.2 6.1 7.0 7.9 8.7 T89-33 3.9 4.4 5.6 6.5 5.7 7.7 T89-34 4.5 5.7 6.6 8.3 9.0 9.5 T89-35 4.2 5.4 7.1 8.5 9.3 10.0 T89-36 5.0 5.6 7.0 8.3 7.1 8.9 T89-37 5.1 6.0 6.9 8.1 9.1 9.6 [00198] PCR in time real was used to trust rm to superex-

construction pressure TF0002. Table 1.4c contains the levels of gene expression of the construction gene in relation to expression of the reference gene. All ratios between the expression levels of the construct and the reference gene shown are normalized to the average of the ratios of the wild type groups. All individuals in the TF0002 construction group are overexpressed according to the present RT-PCR data.

Table 1.4c - Real-time PCR data for TF0002

Sample Level of relative expression of the normalized gene in relation to the mean of the wild type TF0002-1B 8.63 TF0002-2A 7.88 TF0002-2B 8.18 TF0002-3A 5.88 TF0002-3B 5.72 TF0002-4B 8.89 T89-06 1.09 T89-26 1.47 T89-29 0.88 T89-31 0.84 T89-32 0.71

[00199] The results of the growth analysis are specified in table 1.4d of overview. The determined growth effects of the specified construction group are presented as reasons

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Maximum growth rate in diameter 0.85 Maximum maximum growth rate 1.13 Maximum final diameter 0.81 Final maximum height 1.04 Average growth rate in diameter 1.06 Average maximum growth rate 1.31 Final average diameter 0.90 Average height Final 1.12 Construction group TF0002

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Construction group TF0052 [00200] This construction induces increased growth. The final height is 24% higher when comparing the average of the construction group with the control group of the wild type. Construction group TF0052 meets the most stringent level of growth difference selection criteria (4), as shown in Table 1.5d.

[00201] Tables 1.5a and 1.5b contain growth data for the specified construction group and the corresponding wild type group. The columns in the tables contain the height and diameter measurements of individuals from the specified construction group and the corresponding wild type group. The measurement time, in the form of number of days in the greenhouse, is shown in the table headings.

Table 1.5a height growth data for TF0052

Days in the greenhouse 19 28 34 40 Height (cm) 44 47 51 54 61 65 TF0052-1A 8 17 26 35 39 42 48 51 58 64 TF0052-1B 18 32 50 65 79 89 99 107 125 135 TF0052-2A 18 40 64 83 97 108 122 131 156 172 TF0052-2B 13 29 47 59 72 80 90 99 120 132 TF0052-3A 18 36 58 71 84 91 102 107 119 124 TF0052-3B 15 33 54 69 82 90 100 107 124 135 TF0052-4A 14 27 46 58 68 76 85 93 110 122 TF0052-4B 19 36 55 68 79 87 96 98 113 121 T89-01 18 30 46 58 69 77 87 96 113 122 T89-02 18 30 49 62 72 77 84 90 102 109 T89-03 15 27 41 54 65 73 82 91 112 123 T89-10 AT AT AT AT AT AT AT AT AT AT T89-11 19 31 49 61 74 83 94 103 124 133 T89-12 17 30 45 58 69 77 90 100 123 134 T89-13 18 27 43 56 68 78 91 100 121 133 T89-14 5 26 42 56 67 74 83 90 109 119 T89-15 10 15 25 33 41 45 52 57 72 82 T89-16 19 33 53 63 73 82 93 102 119 130 T89-17 17 29 46 58 66 72 80 86 96 102 T89-18 16 30 48 59 71 81 93 103 122 132

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Days in the greenhouse 19 28 34 40 Height (cm) 44 47 51 54 61 65 T89-19 18 32 50 65 77 84 94 102 126 139 T89-20 16 33 52 67 79 88 98 105 124 139 T89-21 17 29 44 58 67 73 83 90 110 122 T89-22 14 28 47 61 71 80 89 98 119 133 T89-23 10 33 45 53 63 71 82 90 112 123 T89-24 20 28 43 51 62 69 78 87 106 119 T89-25 14 26 38 52 64 72 82 89 110 122 T89-26 15 28 44 57 69 77 87 96 120 133 T89-27 18 29 47 62 75 82 92 103 125 138 T89-28 22 37 54 67 78 88 97 104 123 133 T89-29 16 33 49 63 76 84 93 100 123 138 T89-30 15 40 46 58 67 74 85 92 113 124

Table 1.5b - Diameter growth data for TF0052

Days in the greenhouse 28 34 40 Diameter (mm) 44 47 51 54 61 65 TF0052-1A 3.5 4.1 4.9 4.6 5.2 5.0 5.6 6.7 6.2 TF0052-1B 3.1 4.4 6.0 6.6 6.9 8.0 8.2 9.3 9.4 TF0052-2A 4.5 6.3 8.1 8.6 9.7 9.8 10.2 11.5 11.5 TF0052-2B 3.5 5.2 6.1 7.5 7.8 8.8 9.5 10.9 11.0 TF0052-3A 3.5 5.4 6.7 7.2 7.8 7.9 8.1 8.7 9.3 TF0052-3B 3.8 5.5 7.1 7.2 8.2 8.4 9.0 9.1 9.6 TF0052-4A 3.4 4.9 6.5 6.7 7.0 7.8 8.0 9.1 9.3 TF0052-4B 3.5 4.9 6.0 7.0 7.2 7.6 7.8 8.1 8.7 T89-01 3.2 4.6 5.4 6.2 6.8 7.9 8.3 9.5 9.3 T89-02 3.4 4.7 5.5 7.3 6.3 6.6 6.9 8.3 7.5 T89-03 3.9 4.4 5.2 6.2 6.4 7.8 7.6 9.7 9.4 T89-10 AT AT AT AT AT AT AT AT AT T89-11 3.4 5.1 6.2 7.6 6.8 6.9 7.7 10.1 9.5 T89-12 2.9 4.7 5.8 6.6 7.8 8.0 8.7 9.1 9.2 T89-13 3.0 4.3 5.4 5.8 6.4 7.6 7.8 8.2 8.8 T89-14 3.0 4.5 5.7 6.3 6.7 7.9 7.8 9.0 8.9 T89-15 AT 2.1 3.0 4.0 4.0 4.5 4.9 5.5 5.6 T89-16 3.5 5.3 6.4 6.6 7.0 7.2 8.3 8.2 9.0 T89-17 3.4 4.6 5.1 5.4 6.0 6.4 6.5 6.8 7.1 T89-18 3.6 5.2 6.0 7.0 7.8 8.2 9.9 10.3 9.7 T89-19 4.2 5.5 6.6 7.7 8.5 8.9 9.5 11.1 12.3

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Diameter (mm)

Days in the greenhouse 28 34 40 44 47 51 54 61 65 T89-20 4.1 5.5 6.6 8.1 9.3 9.6 9.3 10.0 11.1 T89-21 3.1 5.6 5.8 6.7 7.1 7.8 8.4 9.7 10.1 T89-22 3.2 4.4 5.6 6.5 7.5 7.6 7.8 8.9 9.2 T89-23 2.4 4.2 5.1 6.1 6.5 7.5 10.1 9.3 10.2 T89-24 3.2 4.5 5.1 6.3 7.0 7.6 8.1 8.8 9.1 T89-25 3.3 4.3 5.2 5.8 6.5 7.4 7.8 9.3 9.7 T89-26 3.3 4.4 5.5 6.6 7.2 8.1 8.9 9.5 10.5 T89-27 3.3 4.9 6.0 7.8 8.0 8.9 9.7 11.2 11.5 T89-28 4.5 5.7 7.4 7.8 8.5 9.4 9.7 10.2 11.1 T89-29 3.1 4.7 6.3 7.2 7.9 9.2 9.8 11.1 10.7 T89-30 3.0 5.8 6.2 7.7 7.9 8.6 8.2 10.1 10.3

[00202] The results of the growth analysis are specified in general Table 1.5c. The determined growth effects of the specified construction group are presented as ratios between AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC of the construction group and the wild type group.

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Maximum growth rate in diameter 0.91 Maximum maximum growth rate 1.08 Maximum final diameter 0.93 Final maximum height 1.24 Average growth rate in diameter 0.89 Average maximum growth rate 0.95 Final average diameter 0.98 Final average height 1.00 Construction group TF0052

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Construction group TF0065 [00203] This construction induces increased growth. The final height is 8% higher when comparing the average of the construction group with the control group of the wild type. The maximum rate of growth in height is 11% higher. The construction group compared the average of the construction group with the control group of the wild type. TF0065 meets the growth difference selection criterion (1), as shown in Table 1.6c.

[00204] Tables 1.6a and 1.6b contain growth data for the specified construction group and the corresponding wild type group. The columns in the tables contain the height and diameter measurements of individuals from the specified construction group and the corresponding wild type group. The measurement time, in the form of number of days in the greenhouse, is shown in the table headings. Table 1.6a height growth data for TF0065

Height (cm)

Days in the greenhouse 18 22 25 29 32 39 43 46 50 53 57 TF0065-1AA 20 26 33 46 58 88 101 111 123 134 151 TF0065-1AB 23 27 34 48 60 89 106 115 133 146 164 TF0065-1BA 21 27 33 45 56 82 97 107 122 135 153 TF0065-1BB 22 26 32 44 56 84 101 113 130 144 164 TF0065-2B 24 29 37 51 64 96 115 127 145 162 181 TF0065-3A 20 28 33 43 54 79 94 106 124 138 155 TF0065-4B 16 20 28 40 49 73 85 94 108 121 138 T89-01 14 17 22 31 40 64 76 88 102 118 135 T89-02 17 20 25 36 45 69 79 90 104 118 133 T89-03 21 29 35 47 57 81 94 104 119 132 147 T89-04 13 14 17 25 33 45 52 57 70 79 92 T89-05 15 18 24 33 42 69 83 94 106 120 137 T89-06 18 23 29 40 51 78 91 103 120 134 152 T89-07 20 24 30 41 51 74 88 96 109 121 130 T89-08 27 32 40 52 62 88 99 111 124 134 148 T89-09 23 27 34 46 56 83 97 106 122 132 149 T89-10 13 15 20 27 37 59 72 83 98 111 128

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Height (cm)

Days in the greenhouse 18 22 25 29 32 39 43 46 50 53 57 T89-11 AT AT AT AT AT AT AT AT AT AT AT T89-12 26 33 40 54 66 92 107 120 135 149 166 T89-13 21 26 34 45 55 79 94 105 122 134 150 T89-14 18 23 31 42 53 80 97 107 120 128 145 T89-15 26 33 40 53 63 88 103 111 125 138 154 T89-16 27 32 39 51 63 89 104 116 132 146 162 T89-17 17 21 27 38 45 72 87 97 110 124 139 T89-18 23 30 35 46 55 80 94 105 117 131 145 T89-19 23 29 35 45 54 78 91 102 114 125 137 T89-20 22 28 34 49 60 87 100 110 125 137 155 T89-21 27 31 35 42 48 66 77 87 100 110 124 T89-22 13 16 23 32 48 67 81 92 105 118 134 T89-23 23 29 35 48 58 83 97 107 122 136 154 T89-24 23 29 36 48 59 86 101 113 128 141 159 T89-25 16 21 28 40 51 80 97 107 122 137 153 T89-26 27 35 40 54 66 93 107 118 132 144 162 T89-27 26 31 38 49 59 82 96 106 120 131 146 T89-28 25 29 36 49 59 83 97 106 119 131 147 T89-29 26 32 38 51 62 88 103 113 128 142 159 T89-30 24 29 36 48 56 80 93 104 117 131 145 T89-31 24 31 36 47 57 81 94 105 117 131 144 T89-32 26 32 38 49 58 83 98 107 120 134 148 T89-33 24 29 36 48 57 85 100 112 124 134 149 T89-34 21 26 32 43 53 79 90 102 117 131 148 T89-35 21 28 36 46 55 80 94 105 120 133 149 T89-36 16 21 28 39 51 74 88 100 115 128 144 T89-37 28 35 41 52 63 88 101 111 125 137 154 T89-38 27 32 39 51 62 86 101 113 128 140 155 T89-39 21 26 33 46 55 83 101 112 126 139 156 T89-40 21 26 33 45 55 77 88 99 113 124 139 T89-41 13 16 23 32 42 68 85 95 107 118 133 T89-42 24 30 37 49 61 86 101 113 128 143 150 T89-43 25 31 38 51 61 86 102 114 130 144 163 T89-44 23 31 39 51 64 90 105 118 135 151 169 T89-45 26 32 37 49 58 85 100 110 124 137 153 T89-46 20 25 34 43 55 81 97 109 122 133 149

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Table 1.6b - Diameter growth data for TF0065

Diameter (mm)

Days in the greenhouse 29 32 39 43 46 50 53 57 TF0065-1AA 3.9 5.0 6.2 6.7 6.9 7.3 8.5 8.3 TF0065-1AB 4.0 5.1 6.9 8.4 8.0 9.5 9.5 10.6 TF0065-1BA 4.3 4.9 5.9 6.3 6.8 7.5 7.8 8.4 TF0065-1BB 3.8 4.8 6.0 7.1 7.4 8.4 9.3 10.1 TF0065-2B 5.9 4.8 7.1 8.0 8.6 9.5 9.7 10.2 TF0065-3A 4.2 4.9 6.0 6.9 7.1 7.6 8.4 9.2 TF0065-4B 3.3 4.0 4.8 5.5 5.8 AT 6.9 7.9 T89-01 3.2 3.7 5.3 6.2 6.9 7.9 8.3 8.7 T89-02 3.2 3.9 5.1 6.4 7.0 7.6 8.6 8.6 T89-03 4.2 5.3 6.4 6.9 6.9 AT 8.8 9.8 T89-04 2.2 3.0 4.2 4.5 5.4 5.0 5.4 6.1 T89-05 3.0 3.7 4.8 5.9 6.4 7.0 7.6 7.9 T89-06 3.6 4.8 6.5 7.6 8.7 9.1 9.6 10.1 T89-07 3.8 4.9 6.8 7.6 8.0 8.6 9.0 9.7 T89-08 4.4 5.8 6.2 7.3 7.6 8.5 9.4 9.7 T89-09 4.5 5.5 6.5 7.1 7.6 9.1 9.4 9.8 T89-10 3.7 3.9 5.0 6.1 6.8 7.6 8.5 9.2 T89-11 AT AT AT AT AT AT AT AT T89-12 4.6 5.5 7.3 8.6 8.8 9.6 10.2 10.2 T89-13 3.8 4.6 5.7 6.5 6.6 7.4 8.1 8.6 T89-14 3.7 4.2 5.3 5.6 6.1 7.0 7.1 7.8 T89-15 4.6 5.0 6.4 7.0 7.4 8.0 8.4 9.1 T89-16 5.0 5.5 7.0 7.6 8.0 8.6 9.0 9.7 T89-17 3.1 4.3 5.7 7.1 7.4 7.9 8.4 9.2 T89-18 4.5 5.3 7.8 8.0 8.1 9.2 9.9 10.3 T89-19 4.2 5.8 6.7 8.2 8.5 8.5 9.0 9.5 T89-20 4.0 4.7 6.7 8.1 7.6 8.5 8.6 9.4 T89-21 3.5 3.9 5.0 5.7 5.8 7.1 6.7 7.4 T89-22 3.6 4.3 5.5 6.3 6.8 7.9 8.4 8.8 T89-23 4.1 5.2 7.0 7.6 8.2 8.9 9.2 10.0 T89-24 4.1 5.4 6.7 7.5 7.8 8.6 9.6 10.4 T89-25 3.7 4.6 6.0 7.0 7.7 8.3 8.5 9.4 T89-26 4.8 5.6 6.9 7.3 7.8 8.6 9.2 9.5 T89-27 3.8 4.9 6.2 6.7 6.9 7.6 8.4 9.3 T89-28 4.5 5.5 6.8 7.3 7.8 9.1 9.1 9.5

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Diameter (mm)

Days in the greenhouse 29 32 39 43 46 50 53 57 T89-29 4.4 5.3 6.8 6.8 7.4 8.2 9.2 8.9 T89-30 3.7 4.7 5.4 6.6 7.0 7.0 7.7 8.5 T89-31 4.0 4.5 5.5 6.9 7.0 8.0 9.1 9.6 T89-32 3.6 4.5 5.7 7.0 7.2 8.0 9.1 9.4 T89-33 3.9 4.6 6.7 7.3 8.0 8.6 9.4 10.5 T89-34 3.6 4.5 5.9 6.9 7.5 8.2 9.1 9.4 T89-35 3.9 4.5 5.7 7.1 7.5 7.9 8.8 9.6 T89-36 3.6 5.0 5.7 6.5 6.8 7.9 8.2 9.1 T89-37 4.3 5.6 7.1 8.0 8.0 8.9 9.7 10.2 T89-38 4.7 5.8 6.7 7.8 8.0 8.7 9.2 9.7 T89-39 4.2 5.0 6.2 7.3 8.2 8.1 8.7 9.6 T89-40 3.6 4.4 5.2 5.7 6.0 7.2 7.2 8.0 T89-41 3.5 4.3 5.5 6.5 7.0 7.5 7.7 8.4 T89-42 4.4 5.1 7.5 8.4 9.5 9.9 10.0 10.3 T89-43 4.3 5.0 6.5 7.3 7.8 8.3 8.9 9.1 T89-44 4.3 5.8 6.8 8.1 8.6 9.5 9.9 10.5 T89-45 4.2 4.9 6.8 7.5 7.6 8.5 9.4 9.7 T89-46 3.5 4.4 5.7 7.0 7.5 8.6 9.4 9.8

[00205] The results of the growth analysis are specified in general Table 1.6c. The determined growth effects of the specified construction group are presented as ratios between AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC of the construction group and the wild type group.

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Maximum growth rate in diameter 0.97 Maximum maximum growth rate 1.06 Maximum final diameter 1.01 Final maximum height 1.07 Average growth rate in diameter 0.96 Average maximum growth rate 1.11 Final average diameter 0.99 Final average height 1.08 Construction group TF0065

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Construction group TF0065 [00206] This construction induces increased growth. The final height is 10% higher when comparing the average of the construction group with the control group of the wild type. The maximum rate of growth in height is 18% higher when comparing the average of the construction group with the control group of the wild type. The growth rate at maximum height is 13% higher when comparing the average of the construction group with the control group of the wild type. The growth rate at maximum height is 18% higher when comparing the largest individuals in the construction group with the wild type control group. Construction group TF0076 meets the most stringent growth difference selection criteria (1) and the least stringent growth difference selection criterion (4), as shown in table 1.7d.

[00207] Tables 1.7a and 1.7b contain growth data for the specified construction group and the corresponding wild type group. The columns in the tables contain the height and diameter measurements of individuals from the specified construction group and the corresponding wild type group. The measurement time, in the form of number of days in the greenhouse, is shown in the table headings. Table 1.7a Height growth data for TF0076

Days in the greenhouse 18 22 25 29 Height (cm) 32 39 43 46 50 53 57 TF0076-2AA 18 24 30 42 52 76 92 103 118 133 150 TF0076-2AB 18 25 33 44 55 81 94 104 117 128 143 TF0076-3BA 16 20 26 36 47 70 86 97 112 126 142 TF0076-3BB 23 28 35 49 61 90 104 115 130 143 155 TF0076-4B AT AT AT AT AT AT AT AT AT AT AT TF0076-5BA 20 25 33 45 55 87 106 121 140 157 177 TF0076-5BB 24 32 40 57 71 105 127 140 160 180 200 T89-01 14 17 22 31 40 64 76 88 102 118 135 T89-02 17 20 25 36 45 69 79 90 104 118 133

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Days in the greenhouse 18 22 25 29 Height (cm) 32 39 43 46 50 53 57 T89-03 21 29 35 47 57 81 94 104 119 132 147 T89-04 13 14 17 25 33 45 52 57 70 79 92 T89-05 15 18 24 33 42 69 83 94 106 120 137 T89-06 18 23 29 40 51 78 91 103 120 134 152 T89-07 20 24 30 41 51 74 88 96 109 121 130 T89-08 27 32 40 52 62 88 99 111 124 134 148 T89-09 23 27 34 46 56 83 97 106 122 132 149 T89-10 13 15 20 27 37 59 72 83 98 111 128 T89-11 AT AT AT AT AT AT AT AT AT AT AT T89-12 26 33 40 54 66 92 107 120 135 149 166 T89-13 21 26 34 45 55 79 94 105 122 134 150 T89-14 18 23 31 42 53 80 97 107 120 128 145 T89-15 26 33 40 53 63 88 103 111 125 138 154 T89-16 27 32 39 51 63 89 104 116 132 146 162 T89-17 17 21 27 38 45 72 87 97 110 124 139 T89-18 23 30 35 46 55 80 94 105 117 131 145 T89-19 23 29 35 45 54 78 91 102 114 125 137 T89-20 22 28 34 49 60 87 100 110 125 137 155 T89-21 27 31 35 42 48 66 77 87 100 110 124 T89-22 13 16 23 32 48 67 81 92 105 118 134 T89-23 23 29 35 48 58 83 97 107 122 136 154 T89-24 23 29 36 48 59 86 101 113 128 141 159 T89-25 16 21 28 40 51 80 97 107 122 137 153 T89-26 27 35 40 54 66 93 107 118 132 144 162 T89-27 26 31 38 49 59 82 96 106 120 131 146 T89-28 25 29 36 49 59 83 97 106 119 131 147 T89-29 26 32 38 51 62 88 103 113 128 142 159 T89-30 24 29 36 48 56 80 93 104 117 131 145 T89-31 24 31 36 47 57 81 94 105 117 131 144 T89-32 26 32 38 49 58 83 98 107 120 134 148 T89-33 24 29 36 48 57 85 100 112 124 134 149 T89-34 21 26 32 43 53 79 90 102 117 131 148 T89-35 21 28 36 46 55 80 94 105 120 133 149 T89-36 16 21 28 39 51 74 88 100 115 128 144 T89-37 28 35 41 52 63 88 101 111 125 137 154 T89-38 27 32 39 51 62 86 101 113 128 140 155 T89-39 21 26 33 46 55 83 101 112 126 139 156

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Days in the greenhouse 18 22 25 29 Height (cm) 32 39 43 46 50 53 57 T89-40 21 26 33 45 55 77 88 99 113 124 139 T89-41 13 16 23 32 42 68 85 95 107 118 133 T89-42 24 30 37 49 61 86 101 113 128 143 150 T89-43 25 31 38 51 61 86 102 114 130 144 163 T89-44 23 31 39 51 64 90 105 118 135 151 169 T89-45 26 32 37 49 58 85 100 110 124 137 153 T89-46 20 25 34 43 55 81 97 109 122 133 149

Table 1.7b TF0076 diameter growth data

Days in the greenhouse 29 32 39 Diameter (mm) 43 46 50 53 57 TF0076-2AA 3.8 4.5 6.6 7.5 8.0 9.1 9.9 10.4 TF0076-2AB 4.8 5.0 5.6 6.3 6.8 7.3 7.9 8.1 TF0076-3BA 3.1 3.8 5.5 6.6 7.5 7.6 8.8 8.9 TF0076-3BB 3.8 4.7 6.2 7.4 8.5 8.7 9.4 10.0 TF0076-4B AT AT AT AT AT AT AT AT TF0076-5BA 3.7 4.9 6.1 7.0 7.8 9.1 9.7 9.8 TF0076-5BB 4.2 6.0 7.2 8.9 8.9 9.8 10.1 10.6 T89-01 3.2 3.7 5.3 6.2 6.9 7.9 8.3 8.7 T89-02 3.2 3.9 5.1 6.4 7.0 7.6 8.6 8.6 T89-03 4.2 5.3 6.4 6.9 6.9 AT 8.8 9.8 T89-04 2.2 3.0 4.2 4.5 5.4 5.0 5.4 6.1 T89-05 3.0 3.7 4.8 5.9 6.4 7.0 7.6 7.9 T89-06 3.6 4.8 6.5 7.6 8.7 9.1 9.6 10.1 T89-07 3.8 4.9 6.8 7.6 8.0 8.6 9.0 9.7 T89-08 4.4 5.8 6.2 7.3 7.6 8.5 9.4 9.7 T89-09 4.5 5.5 6.5 7.1 7.6 9.1 9.4 9.8 T89-10 3.7 3.9 5.0 6.1 6.8 7.6 8.5 9.2 T89-11 AT AT AT AT AT AT AT AT T89-12 4.6 5.5 7.3 8.6 8.8 9.6 10.2 10.2 T89-13 3.8 4.6 5.7 6.5 6.6 7.4 8.1 8.6 T89-14 3.7 4.2 5.3 5.6 6.1 7.0 7.1 7.8 T89-15 4.6 5.0 6.4 7.0 7.4 8.0 8.4 9.1

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Diameter (mm)

Days in the greenhouse 29 32 39 43 46 50 53 57 T89-16 5.0 5.5 7.0 7.6 8.0 8.6 9.0 9.7 T89-17 3.1 4.3 5.7 7.1 7.4 7.9 8.4 9.2 T89-18 4.5 5.3 7.8 8.0 8.1 9.2 9.9 10.3 T89-19 4.2 5.8 6.7 8.2 8.5 8.5 9.0 9.5 T89-20 4.0 4.7 6.7 8.1 7.6 8.5 8.6 9.4 T89-21 3.5 3.9 5.0 5.7 5.8 7.1 6.7 7.4 T89-22 3.6 4.3 5.5 6.3 6.8 7.9 8.4 8.8 T89-23 4.1 5.2 7.0 7.6 8.2 8.9 9.2 10.0 T89-24 4.1 5.4 6.7 7.5 7.8 8.6 9.6 10.4 T89-25 3.7 4.6 6.0 7.0 7.7 8.3 8.5 9.4 T89-26 4.8 5.6 6.9 7.3 7.8 8.6 9.2 9.5 T89-27 3.8 4.9 6.2 6.7 6.9 7.6 8.4 9.3 T89-28 4.5 5.5 6.8 7.3 7.8 9.1 9.1 9.5 T89-29 4.4 5.3 6.8 6.8 7.4 8.2 9.2 8.9 T89-30 3.7 4.7 5.4 6.6 7.0 7.0 7.7 8.5 T89-31 4.0 4.5 5.5 6.9 7.0 8.0 9.1 9.6 T89-32 3.6 4.5 5.7 7.0 7.2 8.0 9.1 9.4 T89-33 3.9 4.6 6.7 7.3 8.0 8.6 9.4 10.5 T89-34 3.6 4.5 5.9 6.9 7.5 8.2 9.1 9.4 T89-35 3.9 4.5 5.7 7.1 7.5 7.9 8.8 9.6 T89-36 3.6 5.0 5.7 6.5 6.8 7.9 8.2 9.1 T89-37 4.3 5.6 7.1 8.0 8.0 8.9 9.7 10.2 T89-38 4.7 5.8 6.7 7.8 8.0 8.7 9.2 9.7 T89-39 4.2 5.0 6.2 7.3 8.2 8.1 8.7 9.6 T89-40 3.6 4.4 5.2 5.7 6.0 7.2 7.2 8.0 T89-41 3.5 4.3 5.5 6.5 7.0 7.5 7.7 8.4 T89-42 4.4 5.1 7.5 8.4 9.5 9.9 10.0 10.3 T89-43 4.3 5.0 6.5 7.3 7.8 8.3 8.9 9.1 T89-44 4.3 5.8 6.8 8.1 8.6 9.5 9.9 10.5 T89-45 4.2 4.9 6.8 7.5 7.6 8.5 9.4 9.7 T89-46 3.5 4.4 5.7 7.0 7.5 8.6 9.4 9.8

[00208] Real-time PCR was used to confirm superexpetition 870170074044, of 29/09/2017, pg. 100/378

89/362 pressure of construction TF0076. Table 1.7c contains the expression levels of the construction gene in relation to the expression of the reference gene. All ratios between the expression levels of the construct and the reference gene shown are normalized to the average of the ratios of the wild type groups. Four of the six individuals in the TF0076 construction group are overexpressed according to the present RT-PCR data.

Table 1.7c - Real-time PCR data for TF0076

Sample Level of relative expression of the normalized gene in relation to the mean of the wild type TF0076-2AA 0.43 TF0076-2AB 3.25 TF0076-3BA 3.61 TF0076-3BB 0.65 TF0076-5BA 3.70 TF0076-5BB 3.63 T89-03 1.46 T89-36 1.54 T89-37 0.52 T89-38 0.66 T89-39 0.82

[00209] The results of the growth analysis are specified in Table 1.7d overall. The determined growth effects of the specified construction group are presented as ratios between AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC of the construction group and the wild type group.

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Maximum growth rate in diameter 1.02 Maximum maximum growth rate 00 Maximum final diameter 1.01 Final maximum height 00 Average growth rate in diameter 1.09 Average maximum growth rate 1.13 Final average diameter 1.04 Final average height 1.10 Construction group TF0076

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Construction group TF0089 [00210] This construction induces increased growth. The final height is 10% higher when comparing the average of the construction group with the control group of the wild type. The growth rate at maximum height is 17% higher when comparing the average of the construction group with the control group of the wild type. The maximum height growth rate is 12% higher when comparing the largest individuals in the construction group with the wild type control group. The maximum height growth rate is 12% higher when comparing the largest individuals in the construction group with the wild type control group. Construction group TF0089 meets the most stringent growth difference selection criteria (1), as shown in Table 1.8c.

[00211] Tables 1.8a and 1.8b contain growth data for the specified construction group and the corresponding wild type group. The columns in the tables contain the height and diameter measurements of individuals from the specified construction group and the corresponding wild type group. The measurement time, in the form of number of days in the greenhouse, is shown in the table headings. Table 1.8a Height growth data for TF0089

Height (cm)

Days in the greenhouse 18 21 26 32 35 39 43 46 53 60 64 TF0089-1A 4 7 13 25 35 48 60 71 94 122 140 TF0089-1BA 19 22 31 51 61 71 84 92 115 138 152 TF0089-1BB 10 13 19 34 41 52 67 78 103 125 138 TF0089-2AA 23 29 37 52 61 71 86 97 119 144 158 TF0089-2AB 20 25 33 47 56 68 80 90 113 137 148 TF0089-2B 24 30 32 60 70 80 98 111 140 168 184 T89-01 20 27 35 54 61 72 84 93 112 130 141 T89-02 19 25 31 48 56 67 79 90 113 137 149 T89-03 21 26 33 48 55 64 75 83 100 117 128 T89-04 21 26 33 52 59 69 83 96 120 140 152 T89-05 20 25 34 52 59 70 84 120 144 156

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Height (cm)

Days in the greenhouse 18 21 26 32 35 39 43 46 53 60 64 T89-06 20 26 35 52 61 73 87 98 121 143 156 T89-07 17 21 27 45 53 63 74 85 107 129 140 T89-08 18 24 31 48 56 64 75 86 106 128 141 T89-09 24 28 34 48 55 65 78 91 112 136 153 T89-10 18 24 30 44 52 62 75 87 107 129 142 T89-11 11 14 20 32 41 52 62 72 95 118 132 T89-12 17 24 30 44 52 62 74 85 106 129 141 T89-13 21 28 35 48 56 67 78 85 103 122 134 T89-14 20 25 34 51 60 69 80 90 110 135 148 T89-15 19 24 32 45 52 62 74 84 106 129 142 T89-16 20 24 30 45 53 63 75 86 108 131 141 T89-17 18 23 28 42 50 61 74 82 104 129 143 T89-18 17 22 28 44 53 62 75 85 108 130 144 T89-19 19 24 30 43 50 59 72 84 103 125 140 T89-20 18 23 30 43 50 60 71 80 102 123 136 T89-21 19 24 31 45 53 62 76 87 107 134 149 T89-22 15 19 23 30 39 47 58 66 82 99 109 T89-23 19 24 34 50 60 69 83 94 118 141 154 T89-24 23 27 36 51 61 72 85 95 120 145 157 T89-25 19 21 29 44 52 63 74 84 102 124 138 T89-26 20 25 32 45 53 63 75 85 108 128 139 T89-27 22 25 33 48 56 66 80 92 112 134 147 T89-28 21 28 33 47 57 68 80 89 111 137 148 T89-29 18 23 28 43 51 61 73 84 105 125 136 T89-30 19 20 25 38 45 53 63 71 91 114 125 T89-31 15 21 29 48 57 66 80 91 114 134 148 T89-32 20 24 33 50 55 65 77 88 110 134 146 T89-33 20 26 33 49 57 68 79 89 113 135 149 T89-34 19 25 33 51 58 70 84 98 120 140 154 T89-35 19 24 31 46 54 64 78 90 112 135 147 T89-36 21 25 33 49 57 68 80 89 109 130 140 T89-37 17 23 32 49 58 68 78 88 110 133 146 T89-38 18 24 31 46 54 65 79 91 112 138 152 T89-39 20 25 32 50 59 69 82 92 118 138 151 T89-40 22 27 35 49 56 66 77 88 109 131 145 T89-41 20 24 30 44 51 60 73 82 106 129 141 T89-42 21 26 32 50 58 68 82 92 115 134 148

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Days in the greenhouse Height (cm) 18 21 26 32 35 39 43 46 53 60 64 T89-43 17 24 32 47 55 65 79 89 113 139 154 T89-44 18 24 31 47 56 65 78 89 112 135 152 T89-45 20 23 29 45 55 63 75 86 106 129 141 T89-46 21 24 32 45 55 67 78 85 103 122 138 T89-47 19 24 30 46 53 67 74 84 105 131 144

Table 1.8b - Diameter growth data for TF0089

Days in the greenhouse 32 35 39 Diameter (mm) 43 46 53 60 64 TF0089-1A 3.0 3.3 4.2 5.1 6.0 7.5 8.3 8.2 TF0089-1BA 4.9 6.0 6.5 7.8 8.1 9.1 9.5 10.1 TF0089-1BB 4.0 4.2 5.3 6.1 6.8 7.6 8.7 9.2 TF0089-2AA 5.7 6.3 7.3 8.6 8.1 9.6 10.9 11.5 TF0089-2AB 4.8 6.0 6.5 7.5 8.1 8.8 10.4 10.4 TF0089-2B 5.3 6.0 6.8 8.6 9.4 10.0 12.4 12.2 T89-01 5.1 5.8 6.6 7.4 8.5 8.3 9.5 9.7 T89-02 5.1 5.8 6.7 7.5 8.6 9.5 10.9 11.4 T89-03 4.6 5.3 5.8 6.4 7.0 7.5 8.2 8.7 T89-04 5.4 6.2 7.3 8.1 8.6 9.8 10.8 10.9 T89-05 5.1 6.0 7.1 7.7 8.7 9.3 10.7 11.0 T89-06 5.6 5.6 6.8 7.4 8.6 10.3 10.8 11.3 T89-07 4.1 4.8 5.6 6.7 7.3 8.5 9.8 9.9 T89-08 4.8 5.5 6.2 7.5 7.4 8.2 9.0 9.0 T89-09 5.1 5.5 6.5 7.6 7.9 9.7 10.6 10.8 T89-10 5.8 5.8 6.5 7.2 7.5 9.3 10.2 10.9 T89-11 3.8 4.4 5.5 6.1 6.7 8.6 9.9 10.1 T89-12 4.8 5.8 6.2 7.0 6.9 10.0 9.3 9.7 T89-13 5.9 5.8 7.3 8.7 9.0 10.7 11.2 11.6 T89-14 5.4 6.3 6.7 8.5 8.6 10.0 10.8 11.3 T89-15 5.0 5.5 6.6 7.3 8.1 9.7 10.3 10.3 T89-16 4.8 5.3 5.9 6.8 7.6 8.1 9.8 10.0 T89-17 4.0 4.7 6.1 6.6 6.8 9.0 9.6 10.5 T89-18 4.7 5.7 6.5 7.2 7.8 8.8 9.0 9.8 T89-19 4.5 5.4 6.1 6.8 7.2 8.4 9.8 10.1 T89-20 5.4 5.9 7.3 7.9 8.6 9.8 11.2 11.6 T89-21 5.0 5.8 5.7 8.2 8.0 9.3 10.1 11.2 T89-22 3.1 3.6 3.8 4.2 4.1 5.0 5.3 5.5

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Diameter (mm)

Days in the greenhouse 32 35 39 43 46 53 60 64 T89-23 5.2 5.8 6.7 7.8 8.1 10.2 11.5 12.3 T89-24 5.3 6.2 7.0 7.7 8.0 9.2 9.9 10.9 T89-25 4.6 5.0 6.0 6.7 7.0 9.0 8.9 9.3 T89-26 5.2 4.9 5.9 6.3 7.0 7.7 9.5 9.7 T89-27 4.6 5.3 6.0 6.8 7.3 9.0 9.8 10.9 T89-28 4.7 5.7 6.1 6.7 7.8 9.1 10.3 10.6 T89-29 4.5 4.9 5.7 7.4 7.3 7.6 8.5 9.4 T89-30 3.7 4.9 5.3 6.0 6.3 7.6 8.5 9.3 T89-31 5.2 6.1 7.1 8.3 8.2 9.4 10.2 10.7 T89-32 5.1 5.9 7.0 7.8 8.5 9.7 10.6 12.1 T89-33 4.9 5.6 6.7 7.8 8.6 10.0 10.3 11.1 T89-34 7.0 6.7 7.9 9.1 9.6 11.0 11.3 12.1 T89-35 4.9 5.4 6.8 7.5 8.5 11.1 10.3 10.9 T89-36 5.3 6.3 6.5 7.2 7.3 8.1 9.5 9.8 T89-37 5.0 5.7 6.4 6.9 6.9 7.7 9.2 9.1 T89-38 4.4 4.9 6.2 7.2 7.6 9.0 10.1 11.3 T89-39 4.8 5.1 6.2 6.6 7.5 8.5 9.7 10.2 T89-40 5.1 5.8 6.9 7.4 7.8 9.0 10.3 10.7 T89-41 4.2 5.3 6.1 7.2 8.0 8.9 10.4 10.5 T89-42 4.9 6.1 6.2 7.2 8.0 9.2 10.2 10.7 T89-43 4.6 5.8 6.4 7.8 7.8 9.6 11.1 11.5 T89-44 5.0 5.6 6.3 7.0 7.4 9.0 10.9 10.6 T89-45 5.0 5.1 5.4 6.8 6.2 7.9 8.7 9.3 T89-46 5.2 5.8 6.7 7.6 8.2 9.4 10.8 11.4 T89-47 4.6 5.8 6.3 7.3 8.2 9.1 9.8 10.3

[00212] The results of the growth analysis are specified in general Table 1.8c. The determined growth effects of the specified construction group are presented as ratios between AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC of the construction group and the wild type group.

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Maximum growth rate in diameter 0.96 Maximum maximum growth rate 1.17 Maximum final diameter 0.99 Final maximum height 1.17 Average growth rate in diameter 1.02 Average maximum growth rate 1.12 Final average diameter 0.98 Final average height 1.07 Construction group TF0089

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Construction group TF0109 [00213] This construction induces increased growth. The final height is 24% higher when comparing the average of the construction group with the control group of the wild type. The final height is 39% higher when comparing the tallest individuals in the construction group and the wild type control group. The growth rate at maximum height is 27% higher when comparing the average of the construction group with the control group of the wild type. The maximum height growth rate is 44% higher when comparing the largest individuals in the construction group with the wild type control group. Construction group TF0089 meets the most stringent growth difference selection criteria (1), (3) and (4), as shown in Table 1.9d.

[00214] Tables 1.9a and 1.9b contain growth data for the specified construction group and the corresponding wild type group. The columns in the tables contain the height and diameter measurements of individuals from the specified construction group and the corresponding wild type group. The measurement time, in the form of number of days in the greenhouse, is shown in the table headings.

Table 1.9a Height growth data for TF0109

Greenhouse days 17 20 27 Height (cm) 34 41 48 56 TF0109-1B-1 17 20 34 56 81 108 138 TF0109-1B-2 19 23 42 62 88 113 142 TF0109-2A 16 21 39 59 87 118 150 TF0109-2B 19 25 45 75 121 157 185 TF0109-3B 19 25 46 70 96 123 159 TF0109-4A 15 20 36 56 81 105 133 TF0109-4B 20 28 51 84 122 162 210 T89-01 18 20 29 46 65 87 111 T89-02 20 23 38 54 75 98 122

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Greenhouse days 17 20 27 Height (cm) 34 41 48 56 T89-03 16 19 32 49 72 95 124 T89-04 19 24 40 57 81 103 133 T89-05 18 22 35 52 71 95 124 T89-06 16 21 33 53 77 100 132 T89-07 18 22 37 57 82 107 138 T89-08 12 14 25 42 64 87 117 T89-09 12 16 33 52 75 101 128 T89-10 16 20 31 51 75 100 133 T89-11 12 17 31 52 75 98 122 T89-12 17 22 39 43 AT AT AT T89-13 18 23 39 59 83 110 145 T89-14 19 23 40 61 81 100 127 T89-15 19 21 33 51 77 99 127 T89-16 21 24 40 57 82 107 140 T89-17 20 24 37 56 77 103 136 T89-18 19 24 39 58 83 110 140 T89-19 20 24 35 51 76 102 132 T89-20 19 23 39 59 82 111 146 T89-21 21 24 37 57 81 107 136 T89-22 20 24 39 61 85 110 142 T89-23 19 22 37 54 77 102 128 T89-24 17 22 35 55 80 107 140 T89-25 19 23 38 57 78 101 131 T89-26 18 20 35 53 71 99 128 T89-27 23 27 42 59 85 106 129 T89-28 19 25 37 55 76 96 126 T89-29 12 13 19 29 46 66 91 T89-30 19 23 36 58 81 107 136 T89-31 19 21 33 48 71 95 124 T89-32 16 20 31 48 71 97 126 T89-33 19 22 36 56 78 103 132

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Greenhouse days 17 20 27 Height (cm) 34 41 48 56 T89-34 18 21 36 52 74 97 120 T89-35 20 22 36 54 76 97 125 T89-36 21 28 43 65 87 115 151 T89-37 16 19 32 50 73 97 126 T89-38 17 21 35 52 74 92 113 T89-39 17 19 28 45 60 89 116 T89-40 21 26 38 55 79 104 130

Table 1.9b TF0109 diameter growth data

Greenhouse days 27 34 Diameter (mm) 41 48 56 TF0109-1B-1 3.9 5.2 6.9 9.0 10.5 TF0109-1B-2 4.3 5.7 7.2 8.8 9.8 TF0109-2A 4.1 5.8 7.9 9.9 11.1 TF0109-2B 4.0 5.8 7.2 8.8 9.4 TF0109-3B 4.4 6.0 7.9 9.6 10.2 TF0109-4A 4.2 5.4 7.5 8.4 10.0 TF0109-4B 4.2 5.5 7.1 8.6 10.0 T89-01 3.5 4.3 5.4 7.0 8.1 T89-02 4.2 6.0 7.6 8.0 9.7 T89-03 3.6 5.9 7.0 8.0 9.9 T89-04 4.0 6.0 7.7 8.9 10.7 T89-05 4.0 5.6 7.4 9.0 10.4 T89-06 4.7 6.2 7.8 9.9 11.2 T89-07 5.3 5.8 7.0 8.4 10.0 T89-08 4.0 4.6 6.4 7.8 9.4 T89-09 4.2 4.9 7.0 9.4 10.5 T89-10 3.9 5.0 7.0 9.0 9.7 T89-11 3.8 5.0 6.6 7.7 9.1 T89-12 4.8 AT AT AT AT T89-13 4.0 5.5 7.2 8.9 10.1 T89-14 4.4 5.8 6.9 8.2 8.0

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Greenhouse days 27 34 Diameter (mm) 41 48 56 T89-15 3.6 4.6 6.5 7.4 8.7 T89-16 4.0 5.3 6.4 8.8 9.3 T89-17 3.8 6.4 6.7 8.5 9.6 T89-18 4.3 5.8 6.9 8.1 9.7 T89-19 3.7 5.0 6.9 7.9 9.3 T89-20 3.9 5.2 7.1 8.4 9.8 T89-21 4.3 5.4 7.0 8.5 11.0 T89-22 4.3 5.6 7.0 9.0 10.0 T89-23 4.0 5.2 7.3 8.6 10.8 T89-24 4.6 5.8 7.5 8.5 10.0 T89-25 4.0 5.9 6.7 7.3 8.8 T89-26 4.2 5.4 6.4 8.0 8.4 T89-27 4.2 5.1 6.5 7.5 7.7 T89-28 3.6 4.5 6.4 7.5 8.9 T89-29 AT 2.5 3.4 4.9 5.9 T89-30 4.2 5.2 6.6 8.0 9.4 T89-31 3.8 4.9 6.5 8.3 9.7 T89-32 3.9 4.8 6.0 7.5 9.2 T89-33 3.7 5.0 7.7 7.8 9.4 T89-34 3.9 5.1 6.2 7.5 7.5 T89-35 3.6 5.5 6.7 7.6 8.9 T89-36 4.1 5.6 7.3 8.8 10.4 T89-37 3.8 4.9 6.9 7.8 9.5 T89-38 3.4 4.6 5.4 5.9 6.5 T89-39 2.8 4.0 5.4 6.2 7.2 T89-40 3.7 5.8 6.4 8.3 9.2

[00215] Real-time PCR was used to confirm overexpression of construction TF0109. Table 1.9c contains the expression levels of the construction gene in relation to the expression of the reference gene. All ratios between the expression levels of the construct and the reference gene shown are normalized to the

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Average 100/362 of the ratios of the wild type groups. Four of the seven individuals in the TF0109 construction group are overexpressed according to the present RT-PCR data. Two of the seven individuals in the TF0109 construction group are downregulated according to the present RT-PCR data. Individuals with higher levels of expression of the TF109 construct grow faster and are taller correlatively, whereas individuals with lower levels of expression of the TF0109 construct are shorter.

Table 1.9c Real-time PCR data for TF0109

Sample Level of relative expression of the normalized gene in relation to the mean of the wild type TF0109-1B-1 0.39 TF0109-1B-2 0.55 TF0109-2A 2.16 TF0109-2B 9.21 TF0109-3B 1.80 TF0109-4A 0.95 TF0109-4B 6.24 T89-03 1.08 T89-07 1.17 T89-08 1.11 T89-10 0.79 T89-11 0.85

[00216] The results of the growth analysis are specified in Table 1.9d overall. The determined growth effects of the specified construction group are presented as ratios between AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC of the construction group and the wild type group.

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Maximum growth rate in diameter 1.02 Maximum maximum growth rate Maximum final diameter 1.00 Final maximum height 1.39 Average growth rate in diameter 1.12 Average maximum growth rate 1.27 Final average diameter 1.09 Final average height 1.24 Construction group TF0109

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Construction group TF0132 [00217] This construction induces increased growth. The final height is 13% higher when comparing the average of the construction group with the control group of the wild type. The final height is 26% higher when comparing the tallest individuals in the construction group and the wild type control group. The growth rate at maximum height is 18% higher when comparing the average of the construction group with the control group of the wild type. The growth rate at maximum height is 18% higher when comparing the largest individuals in the construction group with the wild type control group. Construction group TF0132 meets the growth difference selection criteria at the most stringent level of criteria (1) and (4) growth difference selection as shown in Table 10.c;

[00218] Tables 1.10a and 1.10b contain growth data for the specified construction group and the corresponding wild type group. The columns in the tables contain the height and diameter measurements of individuals from the specified construction group and the corresponding wild type group. The measurement time, in the form of number of days in the greenhouse, is shown in the table headings. Table 1.10a Height growth data for TF0132

Height (cm)

Greenhouse days 20 28 34 37 41 48 51 55 59 TF0132-3A-1 23 38 53 60 70 89 96 107 119 TF0132-3A-2 15 28 42 49 58 75 81 93 100 TF0132-3A-3 26 41 54 61 72 89 96 107 119 TF0132-3B-1 27 47 63 73 84 113 125 142 156 TF0132-3B-2 30 51 71 80 94 AT 134 148 160 TF0132-3BB 32 58 87 96 112 141 155 171 182 TF0132-4AA 20 39 59 67 78 102 112 129 143 TF0132-4AB 21 43 63 74 87 114 126 142 156 TF0132-4AC 27 48 67 76 89 117 129 146 156

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Height (cm)

Greenhouse days 20 28 34 37 41 48 51 55 59 T89-02 27 40 51 58 66 86 94 105 115 T89-03 27 44 58 67 77 97 104 114 126 T89-04 27 39 56 65 74 92 100 109 118 T89-07 28 47 66 77 88 107 116 130 140 T89-08 19 37 51 59 69 90 99 109 119 T89-09 25 43 58 67 78 100 109 121 131 T89-11 29 45 60 69 81 104 111 121 132 T89-12 26 41 58 65 77 105 114 127 136 T89-13 31 47 70 78 90 110 119 131 143 T89-14 26 46 70 78 88 108 116 127 137 T89-18 22 41 55 65 74 95 104 115 126 T89-19 27 43 60 70 81 102 110 121 131 T89-20 25 40 57 AT 74 94 103 118 130 T89-21 27 45 61 72 84 108 115 126 137 T89-22 25 38 55 65 77 95 104 115 125 T89-23 23 37 50 57 65 82 92 106 115 T89-24 25 41 54 60 69 87 94 102 110 T89-25 25 38 55 66 80 100 109 121 133 T89-26 24 38 53 61 69 81 87 95 104 T89-27 26 39 54 62 70 90 97 109 120 T89-28 27 46 67 75 86 107 114 126 136 T89-29 24 39 57 64 74 91 98 108 128 T89-31 27 43 57 65 73 91 97 105 113 T89-32 25 41 55 63 75 96 107 121 135 T89-35 25 38 53 62 73 88 96 103 112 T89-36 27 45 67 78 90 113 122 134 145 T89-37 20 43 59 68 79 101 112 124 137 T89-39 25 45 61 69 79 99 108 120 132 T89-40 23 32 43 49 60 76 84 94 104 T89-41 27 45 66 75 87 112 120 133 144 T89-43 26 38 54 61 70 93 100 109 120 T89-46 26 45 66 76 88 109 114 125 133

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Table 1.10b Growth data in diameter for TF0132

Days in the greenhouse 34 41 Diameter (mm) 48 55 59 TF0132-3A-1 6.1 7.3 7.8 9.0 8.9 TF0132-3A-2 5.8 7.3 7.8 9.0 9.0 TF0132-3A-3 6.6 8.5 8.6 9.2 9.7 TF0132-3B-1 6.2 7.0 8.6 9.2 9.7 TF0132-3B-2 5.7 7.3 8.8 9.8 9.2 TF0132-3BB 6.4 7.3 8.0 9.1 9.2 TF0132-4AA 5.3 6.0 7.4 8.8 9.1 TF0132-4AB 5.6 7.0 7.0 8.4 8.4 TF0132-4AC 6.2 7.2 9.1 9.6 10.0 T89-02 5.6 6.4 7.8 9.0 8.4 T89-03 6.6 7.7 8.5 8.9 9.6 T89-04 7.2 7.8 9.1 9.8 9.7 T89-07 7.3 7.9 9.5 9.9 10.1 T89-08 5.5 7.8 8.3 10.0 9.8 T89-09 6.7 7.8 9.3 9.7 9.8 T89-11 6.4 8.1 9.2 9.4 9.7 T89-12 7.0 8.0 9.0 10.1 9.9 T89-13 6.8 8.5 9.1 10.7 10.8 T89-14 6.1 6.9 7.7 8.4 8.8 T89-18 5.5 6.5 7.9 9.5 9.4 T89-19 7.5 8.3 9.0 9.5 10.4 T89-20 7.3 7.1 8.7 9.3 9.7 T89-21 7.0 8.6 9.3 10.3 10.3 T89-22 7.0 7.8 8.4 10.0 9.5 T89-23 5.8 6.8 7.9 9.9 9.7 T89-24 6.1 6.8 8.1 9.0 9.4 T89-25 6.5 8.8 9.5 10.0 10.9 T89-26 5.8 6.7 7.0 7.5 7.7 T89-27 6.3 7.4 8.6 9.0 9.5 T89-28 7.0 8.1 8.8 9.7 10.5

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Days in the greenhouse 34 41 Diameter (mm) 48 55 59 T89-29 7.5 7.4 8.5 10.7 10.0 T89-31 5.6 6.3 7.2 7.6 8.6 T89-32 6.0 7.0 8.2 8.7 9.3 T89-35 5.4 6.4 6.8 7.9 8.0 T89-36 6.7 8.2 8.9 9.6 9.4 T89-37 7.4 8.5 8.6 9.3 10.8 T89-39 7.1 7.9 9.6 10.2 10.2 T89-40 3.9 5.2 5.7 6.8 6.6 T89-41 7.2 8.7 AT 9.7 10.3 T89-43 6.9 7.5 8.6 9.7 10.4 T89-46 6.4 7.2 8.2 8.7 9.7

[00219] The results of the growth analysis are specified in general Table 1.10c. The determined growth effects of the specified construction group are presented as ratios between AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC of the construction group and the wild type group.

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Maximum growth rate in diameter 0.99 Maximum maximum growth rate 00 Maximum final diameter 0.92 Final maximum height 1.26 Average growth rate in diameter 1.04 Average maximum growth rate 00 Final average diameter 0.97 Final average height 1.13 Construction group TF0132

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Construction group TFSTT051 [00220] This construction induces increased growth. The final height is 7% higher when comparing the average of the construction group with the control group of the wild type. The final height is 11% higher when comparing the tallest individuals in the construction group and the wild type control group. The growth rate at maximum height is 5% higher when comparing the average of the construction group with the control group of the wild type. The maximum height growth rate is 10% higher when comparing the largest individuals in the construction group with the wild type control group. Construction group TFSTT051 meets criterion (1) for selecting growth difference, as shown in table 1.11d.

[00221] Tables 1.11a and 1.11b contain growth data for the specified construction group and the corresponding wild type group. The columns in the tables contain the height and diameter measurements of individuals from the specified construction group and the corresponding wild type group. The measurement time, in the form of number of days in the greenhouse, is shown in the table headings. Table 1.11a - Height growth data for TFSTT051

Days in the greenhouse 19 28 34 40 Height (created 44 47 51 54 61 65 TFSTT051-1B 13 24 43 57 69 76 83 89 104 112 TFSTT051-2A 18 33 48 61 72 81 93 103 120 131 TFSTT051-2B 17 28 44 56 67 76 88 96 117 129 TFSTT051-3A 19 31 46 59 70 77 87 96 120 130 TFSTT051-3B 16 33 52 67 79 90 101 112 134 145 TFSTT051-4A 18 36 57 71 86 98 111 119 140 154 TFSTT051-4B- 1 TFSTT051-4B- 18 34 52 67 81 87 96 103 123 136 17 31 51 66 78 85 95 105 124 135

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Days in the greenhouse 19 28 34 40 Height (cm) 54 61 65 44 47 51 2 T89-01 18 30 46 58 69 77 87 96 113 122 T89-02 18 30 49 62 72 77 84 90 102 109 T89-03 15 27 41 54 65 73 82 91 112 123 T89-10 AT AT AT AT AT AT AT AT AT AT T89-11 19 31 49 61 74 83 94 103 124 133 T89-12 17 30 45 58 69 77 90 100 123 134 T89-13 18 27 43 56 68 78 91 100 121 133 T89-14 5 26 42 56 67 74 83 90 109 119 T89-15 10 15 25 33 41 45 52 57 72 82 T89-16 19 33 53 63 73 82 93 102 119 130 T89-17 17 29 46 58 66 72 80 86 96 102 T89-18 16 30 48 59 71 81 93 103 122 132 T89-19 18 32 50 65 77 84 94 102 126 139 T89-20 16 33 52 67 79 88 98 105 124 139 T89-21 17 29 44 58 67 73 83 90 110 122 T89-22 14 28 47 61 71 80 89 98 119 133 T89-23 10 33 45 53 63 71 82 90 112 123 T89-24 20 28 43 51 62 69 78 87 106 119 T89-25 14 26 38 52 64 72 82 89 110 122 T89-26 15 28 44 57 69 77 87 96 120 133 T89-27 18 29 47 62 75 82 92 103 125 138 T89-28 22 37 54 67 78 88 97 104 123 133 T89-29 16 33 49 63 76 84 93 100 123 138 T89-30 15 40 46 58 67 74 85 92 113 124

Table 1.11b Diameter growth data for TFSTT051

Days in the greenhouse 28 Diameter (mm) 65 34 40 44 47 51 54 61 TFSTT051-1B 3.2 4.6 5.6 6.4 6.6 7.3 7.2 7.8 8.0 TFSTT051-2A 3.2 4.5 5.7 6.7 7.4 8.6 9.3 8.8 8.9 TFSTT051-2B 3.1 4.4 6.0 6.0 6.8 8.0 8.2 9.0 9.2

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Days in the greenhouse

TFSTT051-3A

TFSTT051-3B

TFSTT051-4A

TFSTT051-4B1

TFSTT051-4B2

T89-01

T89-02

T89-03

T89-10

T89-11

T89-12

T89-13

T89-14

T89-15

T89-16

T89-17

T89-18

T89-19

T89-20

T89-21

T89-22

T89-23

T89-24

T89-25

T89-26

T89-27

T89-28

T89-29

T89-30 [00222] PCR

28 34 40 Diameter (mm) 44 47 51 54 61 65 3.5 5.0 6.1 6.6 7.1 8.2 8.7 9.8 10.5 4.2 5.6 6.8 8.0 8.5 9.4 9.8 10.2 11.1 4.5 5.9 7.1 8.8 8.7 9.4 10.1 11.1 11.1 4.1 5.6 6.8 7.9 8.1 8.6 9.7 10.7 11.6 3.9 5.5 6.3 7.6 8.2 9.4 8.8 9.6 10.3 3.2 4.6 5.4 6.2 6.8 7.9 8.3 9.5 9.3 3.4 4.7 5.5 7.3 6.3 6.6 6.9 8.3 7.5 3.9 4.4 5.2 6.2 6.4 7.8 7.6 9.7 9.4 AT AT AT AT AT AT AT AT AT 3.4 5.1 6.2 7.6 6.8 6.9 7.7 10.1 9.5 2.9 4.7 5.8 6.6 7.8 8.0 8.7 9.1 9.2 3.0 4.3 5.4 5.8 6.4 7.6 7.8 8.2 8.8 3.0 4.5 5.7 6.3 6.7 7.9 7.8 9.0 8.9 AT 2.1 3.0 4.0 4.0 4.5 4.9 5.5 5.6 3.5 5.3 6.4 6.6 7.0 7.2 8.3 8.2 9.0 3.4 4.6 5.1 5.4 6.0 6.4 6.5 6.8 7.1 3.6 5.2 6.0 7.0 7.8 8.2 9.9 10.3 9.7 4.2 5.5 6.6 7.7 8.5 8.9 9.5 11.1 12.3 4.1 5.5 6.6 8.1 9.3 9.6 9.3 10.0 11.1 3.1 5.6 5.8 6.7 7.1 7.8 8.4 9.7 10.1 3.2 4.4 5.6 6.5 7.5 7.6 7.8 8.9 9.2 2.4 4.2 5.1 6.1 6.5 7.5 10.1 9.3 10.2 3.2 4.5 5.1 6.3 7.0 7.6 8.1 8.8 9.1 3.3 4.3 5.2 5.8 6.5 7.4 7.8 9.3 9.7 3.3 4.4 5.5 6.6 7.2 8.1 8.9 9.5 10.5 3.3 4.9 6.0 7.8 8.0 8.9 9.7 11.2 11.5 4.5 5.7 7.4 7.8 8.5 9.4 9.7 10.2 11.1 3.1 4.7 6.3 7.2 7.9 9.2 9.8 11.1 10.7 3.0 5.8 6.2 7.7 7.9 8.6 8.2 10.1 10.3 in te mpo real was usac it to con secure su | aerex-

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110/362 construction pressure TFSTT051. Table 1.11c contains the expression levels of the construction gene in relation to the expression of the reference gene. All ratios between the expression levels of the construct and the reference gene shown are normalized to the average of the ratios of the wild type groups. One of the eight individuals in the TFSTT051 construction group is overexpressed according to the present RT-PCR data.

Table 1.11c Real-time PCR data for TFSTT051

Sample Level of relative expression of the normalized gene in relation to the mean of the wild type TFSTT051-1B 1.18 TFSTT051-2A 7.42 TFSTT051-2B 1.13 TFSTT051-3A 0.30 TFSTT051-3B 1.06 TFSTT051-4A 0.65 TFSTT051-4B-1 1.34 TFSTT051-4B-2 0.76 T89-11 0.42 T89-12 1.65 T89-24 1.27 T89-25 0.66

[00223] The results of the growth analysis are specified in Table 1.11d in general. The determined growth effects of the specified construction group are presented as ratios between AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC of the construction group and the wild type group.

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Maximum growth rate in diameter 0.90 Maximum maximum growth rate 1.10 Maximum final diameter 0.94 Final maximum height 1.11 Average growth rate in diameter 1.01 Average maximum growth rate 1.05 Final average diameter 1.06 Final average height 1.07 Construction group TFSTT051

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Construction group TF0013 [00224] This construction induces increased growth. The final height is 12% higher when comparing the average of the construction group with the control group of the wild type. The final height is 6% higher when comparing the tallest individuals in the construction group and the wild type control group. The growth rate at maximum height is 20% higher when comparing the average of the construction group with the control group of the wild type. The growth rate at maximum height is 33% higher when comparing the largest individuals in the construction group with the wild type control group. Construction group TF0013 meets the most stringent growth difference selection criteria (4), and the least stringent growth criteria selection criteria (1) and (3), as shown in table 1.10c.

[00225] Tables 1.12a and 1.12b contain growth data for the specified construction group and the corresponding wild type group. The columns in the tables contain the height and diameter measurements of individuals from the specified construction group and the corresponding wild type group. The measurement time, in the form of number of days in the greenhouse, is shown in the table headings. Table 1.12a Height growth data for TF0013

Days in the greenhouse 18 22 25 29 Height (cm) 32 39 43 46 50 53 57 TF0013-1A-1 19 24 31 43 53 81 98 111 128 144 161 TF0013-1A-2 12 15 19 27 37 62 77 90 108 125 159 TF0013-2A AT AT AT AT AT AT AT AT AT AT AT TF0013-2B 20 25 32 45 57 85 102 112 128 142 157 TF0013-3A 19 25 33 47 58 88 103 116 134 151 170 TF0013-3BA 24 30 37 52 64 94 112 125 144 159 179 TF0013-3BB 19 26 31 43 53 84 100 114 130 144 164

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Days in the greenhouse 18 22 Height (cm) 53 57 25 29 32 39 43 46 50 TF0013-4BA 25 31 40 52 63 89 107 117 130 141 155 TF0013-4BB 20 25 31 45 57 83 100 111 128 145 162 T89-01 14 17 22 31 40 64 76 88 102 118 135 T89-02 17 20 25 36 45 69 79 90 104 118 133 T89-03 21 29 35 47 57 81 94 104 119 132 147 T89-04 13 14 17 25 33 45 52 57 70 79 92 T89-05 15 18 24 33 42 69 83 94 106 120 137 T89-06 18 23 29 40 51 78 91 103 120 134 152 T89-07 20 24 30 41 51 74 88 96 109 121 130 T89-08 27 32 40 52 62 88 99 111 124 134 148 T89-09 23 27 34 46 56 83 97 106 122 132 149 T89-10 13 15 20 27 37 59 72 83 98 111 128 T89-11 AT AT AT AT AT AT AT AT AT AT AT T89-12 26 33 40 54 66 92 107 120 135 149 166 T89-13 21 26 34 45 55 79 94 105 122 134 150 T89-14 18 23 31 42 53 80 97 107 120 128 145 T89-15 26 33 40 53 63 88 103 111 125 138 154 T89-16 27 32 39 51 63 89 104 116 132 146 162 T89-17 17 21 27 38 45 72 87 97 110 124 139 T89-18 23 30 35 46 55 80 94 105 117 131 145 T89-19 23 29 35 45 54 78 91 102 114 125 137 T89-20 22 28 34 49 60 87 100 110 125 137 155 T89-21 27 31 35 42 48 66 77 87 100 110 124 T89-22 13 16 23 32 48 67 81 92 105 118 134 T89-23 23 29 35 48 58 83 97 107 122 136 154 T89-24 23 29 36 48 59 86 101 113 128 141 159 T89-25 16 21 28 40 51 80 97 107 122 137 153 T89-26 27 35 40 54 66 93 107 118 132 144 162 T89-27 26 31 38 49 59 82 96 106 120 131 146 T89-28 25 29 36 49 59 83 97 106 119 131 147

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Height (cm)

Days in the greenhouse 18 22 25 29 32 39 43 46 50 53 57 T89-29 26 32 38 51 62 88 103 113 128 142 159 T89-30 24 29 36 48 56 80 93 104 117 131 145 T89-31 24 31 36 47 57 81 94 105 117 131 144 T89-32 26 32 38 49 58 83 98 107 120 134 148 T89-33 24 29 36 48 57 85 100 112 124 134 149 T89-34 21 26 32 43 53 79 90 102 117 131 148 T89-35 21 28 36 46 55 80 94 105 120 133 149 T89-36 16 21 28 39 51 74 88 100 115 128 144 T89-37 28 35 41 52 63 88 101 111 125 137 154 T89-38 27 32 39 51 62 86 101 113 128 140 155 T89-39 21 26 33 46 55 83 101 112 126 139 156 T89-40 21 26 33 45 55 77 88 99 113 124 139 T89-41 13 16 23 32 42 68 85 95 107 118 133 T89-42 24 30 37 49 61 86 101 113 128 143 150 T89-43 25 31 38 51 61 86 102 114 130 144 163 T89-44 23 31 39 51 64 90 105 118 135 151 169 T89-45 26 32 37 49 58 85 100 110 124 137 153 T89-46 20 25 34 43 55 81 97 109 122 133 149

Table 1.12b - Diameter growth data for TF0013

Diameter (mm)

Days in the greenhouse 29 32 39 43 46 50 53 57 TF0013-1A-1 3.9 5.1 6.6 7.6 8.6 9.8 9.9 10.5 TF0013-1A-2 3.0 2.8 5.5 6.5 7.2 8.3 8.6 8.6 TF0013-2A AT AT AT AT AT AT AT AT TF0013-2B 4.3 5.0 6.4 7.4 8.0 8.5 9.2 10.2 TF0013-3A 4.6 5.4 7.1 8.2 8.7 9.7 10.3 10.7 TF0013-3BA 4.4 5.0 7.2 7.5 9.1 9.1 9.8 10.6 TF0013-3BB 3.8 5.2 6.7 7.5 7.5 8.4 9.0 9.8 TF0013-4BA 4.7 5.7 6.1 6.8 7.4 8.8 9.0 9.6 TF0013-4BB 3.8 4.9 AT 8.4 8.3 9.0 9.6 9.9

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Diameter (mm)

Days in the greenhouse 29 32 39 43 46 50 53 57 T89-01 3.2 3.7 5.3 6.2 6.9 7.9 8.3 8.7 T89-02 3.2 3.9 5.1 6.4 7.0 7.6 8.6 8.6 T89-03 4.2 5.3 6.4 6.9 6.9 AT 8.8 9.8 T89-04 2.2 3.0 4.2 4.5 5.4 5.0 5.4 6.1 T89-05 3.0 3.7 4.8 5.9 6.4 7.0 7.6 7.9 T89-06 3.6 4.8 6.5 7.6 8.7 9.1 9.6 10.1 T89-07 3.8 4.9 6.8 7.6 8.0 8.6 9.0 9.7 T89-08 4.4 5.8 6.2 7.3 7.6 8.5 9.4 9.7 T89-09 4.5 5.5 6.5 7.1 7.6 9.1 9.4 9.8 T89-10 3.7 3.9 5.0 6.1 6.8 7.6 8.5 9.2 T89-11 AT AT AT AT AT AT AT AT T89-12 4.6 5.5 7.3 8.6 8.8 9.6 10.2 10.2 T89-13 3.8 4.6 5.7 6.5 6.6 7.4 8.1 8.6 T89-14 3.7 4.2 5.3 5.6 6.1 7.0 7.1 7.8 T89-15 4.6 5.0 6.4 7.0 7.4 8.0 8.4 9.1 T89-16 5.0 5.5 7.0 7.6 8.0 8.6 9.0 9.7 T89-17 3.1 4.3 5.7 7.1 7.4 7.9 8.4 9.2 T89-18 4.5 5.3 7.8 8.0 8.1 9.2 9.9 10.3 T89-19 4.2 5.8 6.7 8.2 8.5 8.5 9.0 9.5 T89-20 4.0 4.7 6.7 8.1 7.6 8.5 8.6 9.4 T89-21 3.5 3.9 5.0 5.7 5.8 7.1 6.7 7.4 T89-22 3.6 4.3 5.5 6.3 6.8 7.9 8.4 8.8 T89-23 4.1 5.2 7.0 7.6 8.2 8.9 9.2 10.0 T89-24 4.1 5.4 6.7 7.5 7.8 8.6 9.6 10.4 T89-25 3.7 4.6 6.0 7.0 7.7 8.3 8.5 9.4 T89-26 4.8 5.6 6.9 7.3 7.8 8.6 9.2 9.5 T89-27 3.8 4.9 6.2 6.7 6.9 7.6 8.4 9.3 T89-28 4.5 5.5 6.8 7.3 7.8 9.1 9.1 9.5 T89-29 4.4 5.3 6.8 6.8 7.4 8.2 9.2 8.9 T89-30 3.7 4.7 5.4 6.6 7.0 7.0 7.7 8.5 T89-31 4.0 4.5 5.5 6.9 7.0 8.0 9.1 9.6

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Diameter (mm)

Days in the greenhouse 29 32 39 43 46 50 53 57 T89-32 3.6 4.5 5.7 7.0 7.2 8.0 9.1 9.4 T89-33 3.9 4.6 6.7 7.3 8.0 8.6 9.4 10.5 T89-34 3.6 4.5 5.9 6.9 7.5 8.2 9.1 9.4 T89-35 3.9 4.5 5.7 7.1 7.5 7.9 8.8 9.6 T89-36 3.6 5.0 5.7 6.5 6.8 7.9 8.2 9.1 T89-37 4.3 5.6 7.1 8.0 8.0 8.9 9.7 10.2 T89-38 4.7 5.8 6.7 7.8 8.0 8.7 9.2 9.7 T89-39 4.2 5.0 6.2 7.3 8.2 8.1 8.7 9.6 T89-40 3.6 4.4 5.2 5.7 6.0 7.2 7.2 8.0 T89-41 3.5 4.3 5.5 6.5 7.0 7.5 7.7 8.4 T89-42 4.4 5.1 7.5 8.4 9.5 9.9 10.0 10.3 T89-43 4.3 5.0 6.5 7.3 7.8 8.3 8.9 9.1 T89-44 4.3 5.8 6.8 8.1 8.6 9.5 9.9 10.5 T89-45 4.2 4.9 6.8 7.5 7.6 8.5 9.4 9.7 T89-46 3.5 4.4 5.7 7 7.5 8.6 9.4 9.8

[00226] Real-time PCR was used to confirm overexpression of construction TF0013. Table 1.12c contains the expression levels of the construction gene in relation to the expression of the reference gene. All ratios between the expression levels of the construct and the reference gene shown are normalized to the average of the ratios of the wild type groups. Three of the eight individuals in the TF0013 construction group are overexpressed according to the present RT-PCR data.

Table 1.12c- Real-time PCR data for TF0013

Sample Level of relative expression of the normalized gene in relation to the mean of the wild type TF0013-1A-1 0.59 TF0013-1A-2 1.02 TF0013-2B 2.32 TF0013-3A 0.90

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Sample Level of relative expression of the normalized gene in relation to the mean of the wild type TF0013-3BA 0.86 TF0013-3BB 0.80 TF0013-4BA 1.38 TF0013-4BB 1.65 T89-03 1.22 T89-36 1.04 T89-37 0.90 T89-38 0.85

[00227] The results of the growth analysis are specified in Table 1.12d overall. The determined growth effects of the specified construction group are presented as ratios between AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC of the construction group and the wild type group.

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Maximum growth rate in diameter 1.01 Maximum maximum growth rate 1.33 Maximum final diameter 1.02 Final maximum height 1.06 Average growth rate in diameter 1.12 Average maximum growth rate 1.20 Final average diameter 1.07 Final average height 1.12 Construction group TF0013

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1.3.10 Construction group TF0097 [00228] This construction induces increased growth. The final height is 10% higher when comparing the average of the construction group with the control group of the wild type. The final height is 16% higher when comparing the tallest individuals in the construction group and the wild type control group. The growth rate at maximum height is 15% higher when comparing the average of the construction group with the control group of the wild type. The maximum height growth rate is 15% higher when comparing the largest individuals in the construction group with the wild type control group. The growth rate in diameter is 7% higher compared to the average of the construction group and the control group of the wild type. The growth rate in diameter is 21% higher when comparing the larger individuals of the construction group and the control group of the wild type. Construction group TF0097 meets the most stringent level of growth difference selection criterion (1), and the least stringent level of growth difference selection criterion (4), as shown in Table 1.13d.

[00229] Tables 1.13a and 1.13b contain growth data for the specified construction group and the corresponding wild type group. The columns in the tables contain the height and diameter measurements of individuals from the specified construction group and the corresponding wild type group. The measurement time, in the form of number of days in the greenhouse, is shown in the table headings. Table 1.13a Height growth data for TF0097

Days in the greenhouse Height (cm) 16 20 29 34 41 48 TF0097-1A 28 35 71 93 133 176 TF0097-1B 26 36 72 100 137 171 TF0097-2A 19 22 42 57 79 101 TF0097-2B 31 40 79 105 138 175

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Height (cm)

Days in the greenhouse 16 20 29 34 41 48 TF0097-3A-1 28 36 73 96 130 166 TF0097-3A-2 30 38 72 100 141 178 TF0097-4A-1 27 32 48 68 103 140 TF0097-4A-2 28 34 65 85 118 141 TF0097-4B 25 10 23 37 65 92 T89-01 28 35 66 89 122 150 T89-02 25 35 62 80 103 128 T89-03 24 33 61 83 113 142 T89-04 27 34 65 87 120 152 T89-05 27 32 59 80 107 138 T89-06 25 32 61 77 106 135 T89-07 22 28 50 67 92 120 T89-08 24 29 56 75 103 122 T89-09 20 24 45 61 91 115 T89-10 22 28 52 70 99 124 T89-11 26 32 63 80 110 142 T89-12 27 32 60 80 109 132 T89-13 23 30 59 79 107 133 T89-14 26 31 60 79 106 131 T89-15 26 32 58 79 110 133 T89-16 29 32 65 87 121 151 T89-17 25 32 59 79 105 133 T89-18 29 37 67 86 114 136 T89-19 29 35 66 84 111 129 T89-20 27 38 64 83 112 122 T89-21 28 33 58 79 108 132 T89-22 25 33 54 83 113 140 T89-23 24 31 56 75 104 134 T89-24 25 33 58 78 106 136 T89-25 26 33 62 80 111 142 T89-26 28 35 64 81 113 146 T89-27 26 31 60 75 107 140

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Height (cm)

Days in the greenhouse 16 20 29 34 41 48 T89-28 28 34 61 83 116 147 T89-29 19 24 49 70 101 132 T89-30 26 33 57 77 110 140 T89-31 25 35 63 81 115 140 T89-32 28 36 67 89 121 151 T89-33 26 33 63 85 110 126 T89-34 28 35 59 77 107 135 T89-35 26 34 59 77 107 132 T89-36 26 32 61 79 107 136 T89-37 22 23 39 54 78 106 T89-38 27 35 58 74 98 124 T89-39 24 29 53 73 103 128 T89-40 22 27 52 70 101 121 T89-41 27 34 64 81 111 143 T89-42 25 33 63 79 111 142 T89-43 26 33 62 84 117 148 T89-44 26 32 64 82 115 149 T89-45 24 33 60 79 107 134 T89-46 27 34 64 88 121 147 T89-47 24 32 61 78 101 121 T89-48 24 31 62 79 108 140 T89-49 27 35 63 80 109 138 T89-50 26 33 61 80 108 135 T89-51 20 25 52 70 98 116 T89-52 24 28 53 73 99 128 T89-53 21 31 58 75 100 115 T89-54 22 30 54 72 105 133 T89-55 21 27 53 73 105 129 T89-56 29 36 67 84 115 147 T89-57 27 32 60 80 111 139 T89-58 29 38 67 86 119 150 T89-59 25 32 63 86 120 149

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Days in the greenhouse 16 Height (cm) 48 20 29 34 41 T89-60 27 33 62 84 119 154 T89-61 26 35 65 89 124 152 T89-62 24 30 57 79 107 134 T89-63 25 33 62 81 111 134 T89-64 21 29 58 79 110 139 T89-65 23 31 55 70 96 122 T89-66 25 32 61 82 113 142 T89-67 25 30 50 75 105 131 T89-68 22 31 61 80 110 138 T89-69 25 33 56 74 101 128 T89-70 21 28 59 77 105 135 T89-71 20 26 57 73 101 129 T89-72 26 33 62 82 115 144

Table 1.13b Growth data in diameter for TF0097

Days in the greenhouse 29 Diameter (mm) 34 41 48 TF0097-1A 4.7 5.0 6.9 8.2 TF0097-1B 4.2 5.1 6.9 8.2 TF0097-2A 3.0 3.6 4.1 7.9 TF0097-2B 5.9 6.6 7.1 8.6 TF0097-3A-1 4.9 5.9 7.0 8.1 TF0097-3A-2 5.2 6.4 8.1 9.3 TF0097-4A-1 3.9 5.7 5.0 6.2 TF0097-4A-2 4.7 4.8 5.6 6.4 TF0097-4B 2.5 2.9 4.0 4.8 T89-01 5.3 6.3 8.0 8.8 T89-02 4.8 5.3 6.7 7.1 T89-03 5.3 6.4 8.2 8.7 T89-04 5.3 6.5 7.9 9.3 T89-05 5.4 6.5 7.6 8.1 T89-06 5.3 6.0 7.2 8.4 T89-07 4.0 4.8 7.2 7.7 T89-08 4.5 5.4 6.3 7.1 T89-09 3.7 4.7 6.1 7.5 T89-10 4.4 5.5 6.9 7.6

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Diameter (mm)

Days in the greenhouse 29 34 41 48 T89-11 5.4 6.4 7.1 8.0 T89-12 5.5 5.7 6.8 8.1 T89-13 5.2 6.0 6.7 7.4 T89-14 5.3 6.4 6.7 7.9 T89-15 5.1 6.4 6.9 7.8 T89-16 5.2 6.4 7.6 9.2 T89-17 5.0 5.7 6.5 7.9 T89-18 5.8 6.4 7.1 7.7 T89-19 5.4 6.4 7.5 8.0 T89-20 5.6 6.6 8.3 8.6 T89-21 5.0 5.8 6.9 7.8 T89-22 5.4 6.5 8.2 8.9 T89-23 4.7 5.3 7.1 8.0 T89-24 4.8 6.3 6.9 8.4 T89-25 5.0 6.3 6.9 7.8 T89-26 5.6 6.6 7.5 8.5 T89-27 5.5 6.0 7.5 8.8 T89-28 6.0 7.1 7.7 8.8 T89-29 4.2 5.3 6.8 8.5 T89-30 5.5 6.4 8.0 9.3 T89-31 5.7 5.7 8.4 8.9 T89-32 5.4 6.5 7.8 9.0 T89-33 5.6 6.3 6.8 7.4 T89-34 5.1 6.1 7.2 8.1 T89-35 5.6 6.7 7.7 8.2 T89-36 5.4 6.2 7.7 9.4 T89-37 2.8 3.3 4.7 5.5 T89-38 5.1 5.5 7.1 8.4 T89-39 4.9 5.5 6.5 7.4 T89-40 4.9 6.4 7.6 7.6 T89-41 5.6 7 7.3 9.1 T89-42 5.9 6.4 7.7 8.4 T89-43 5.4 6.7 8.2 9.1 T89-44 6.4 6.4 8.1 9.3 T89-45 5.6 6.4 7.8 8.0 T89-46 6.1 6.4 7.9 9.5 T89-47 5.2 5.9 6.5 6.9 T89-48 5.1 6.1 7.3 8.8

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Diameter (mm)

Days in the greenhouse 29 34 41 48 T89-49 5.3 5.9 7.3 7.7 T89-50 5.3 6.8 7.7 8.2 T89-51 4.7 5.7 7 7.5 T89-52 4.9 5.5 6.9 8.2 T89-53 5.1 6.1 6.8 7.5 T89-54 4.7 5.9 6.7 7.7 T89-55 4.8 5.9 7.1 7.9 T89-56 5.4 6.9 7.7 9.1 T89-57 5.3 6.5 7.5 9.2 T89-58 5.5 6.5 8 8.8 T89-59 5.8 6.4 7.7 8.4 T89-60 5.6 6.2 7.9 9.6 T89-61 5.6 7 8.3 9.4 T89-62 5.1 6.3 7.2 7.7 T89-63 5.5 7.1 8 8.4 T89-64 4.7 6.2 8.4 8.1 T89-65 5.3 6.1 6.9 8.2 T89-66 5.2 6 7.2 8.4 T89-67 5.8 6.5 7.7 8.9 T89-68 5 6.1 7.5 8.2 T89-69 5.1 6.4 7.2 9.0 T89-70 5 6.1 6.6 7.4 T89-71 4.9 6.4 7.2 8.7 T89-72 5.8 6.8 7.9 9.1

[00230] Real-time PCR was used to confirm the overexpression of the TF0097 construct. Table 1.13c contains the expression levels of the construction gene in relation to the expression of the reference gene. All ratios between the expression levels of the construct and the reference gene shown are normalized to the average of the ratios of the wild type groups. Two of the nine individuals in the TF0097 construction group are overexpressed according to the present RT-PCR data.

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Table 1.13c Real-time PCR data for TF0097

Sample Level of relative expression of the normalized gene in relation to the mean of the wild type TF0097-1A 1.85 TF0097-1B 1.66 TF0097-2A 1.45 TF0097-2B 1.57 TF0097-3A-1 1.09 TF0097-3A-2 1.71 TF0097-4A-1 0.79 TF0097-4A-2 4.64 TF0097-4B 3.20 T89-03 1.98 T89-17 0.38 T89-19 0.64 [00231] The results growth analysis results are specified

in Table 1.13d overall. The determined growth effects of the specified construction group are presented as ratios between AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC of the construction group and the wild type group.

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Maximum growth rate in diameter 1.21 Maximum maximum growth rate 1.15 Maximum final diameter 0.97 Final maximum height 1.16 Average growth rate in diameter 1.07 Average maximum growth rate 1.15 Final average diameter 0.91 Final average height 1.10 Construction group TF0097

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1.3.11 Construction group TFSTT019 [00232] This construction induces increased growth. The final diameter is 11% comparing the average of the construction group and the control group of the wild type. The final diameter is 8% higher when comparing the larger individuals of the construction group and the control group of the wild type. The growth rate in diameter is 18% higher when comparing the average of the construction group and the control group of the wild type. Construction group TFSTT019 meets the level of criterion (2) for selecting growth differences, as shown in Table 1.14c.

[00233] Tables 1.14a and 1.14b contain growth data for the specified construction group and the corresponding wild type group. The columns in the tables contain the height and diameter measurements of individuals from the specified construction group and the corresponding wild type group. The measurement time, in the form of number of days in the greenhouse, is shown in the table headings. Table 1.14a Height growth data for TFSTT019

Days in the greenhouse 18 22 25 29 Height (cm) 32 39 43 46 50 53 57 TFSTT019-1A AT AT AT AT AT AT AT AT AT AT AT TFSTT019-1BA 18 22 29 39 49 73 84 91 103 115 129 TFSTT019-1BB 21 26 34 46 55 82 99 107 120 132 145 TFSTT019-2A 24 30 37 51 61 88 104 114 128 140 157 TFSTT019-2B 11 14 18 28 37 60 73 86 102 115 132 TFSTT019-3A 23 31 37 50 57 81 94 106 119 129 144 TFSTT019-4BA 27 32 39 49 58 84 99 110 124 137 152 TFSTT019-4BB 21 27 35 47 57 84 99 110 124 137 151 T89-01 14 17 22 31 40 64 76 88 102 118 135 T89-02 17 20 25 36 45 69 79 90 104 118 133 T89-03 21 29 35 47 57 81 94 104 119 132 147 T89-04 13 14 17 25 33 45 52 57 70 79 92 T89-05 15 18 24 33 42 69 83 94 106 120 137 T89-06 18 23 29 40 51 78 91 103 120 134 152 T89-07 20 24 30 41 51 74 88 96 109 121 130

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Days in the greenhouse 18 22 25 29 Height (cm) 32 39 43 46 50 53 57 T89-08 27 32 40 52 62 88 99 111 124 134 148 T89-09 23 27 34 46 56 83 97 106 122 132 149 T89-10 13 15 20 27 37 59 72 83 98 111 128 T89-11 AT AT AT AT AT AT AT AT AT AT AT T89-12 26 33 40 54 66 92 107 120 135 149 166 T89-13 21 26 34 45 55 79 94 105 122 134 150 T89-14 18 23 31 42 53 80 97 107 120 128 145 T89-15 26 33 40 53 63 88 103 111 125 138 154 T89-16 27 32 39 51 63 89 104 116 132 146 162 T89-17 17 21 27 38 45 72 87 97 110 124 139 T89-18 23 30 35 46 55 80 94 105 117 131 145 T89-19 23 29 35 45 54 78 91 102 114 125 137 T89-20 22 28 34 49 60 87 100 110 125 137 155 T89-21 27 31 35 42 48 66 77 87 100 110 124 T89-22 13 16 23 32 48 67 81 92 105 118 134 T89-23 23 29 35 48 58 83 97 107 122 136 154 T89-24 23 29 36 48 59 86 101 113 128 141 159 T89-25 16 21 28 40 51 80 97 107 122 137 153 T89-26 27 35 40 54 66 93 107 118 132 144 162 T89-27 26 31 38 49 59 82 96 106 120 131 146 T89-28 25 29 36 49 59 83 97 106 119 131 147 T89-29 26 32 38 51 62 88 103 113 128 142 159 T89-30 24 29 36 48 56 80 93 104 117 131 145 T89-31 24 31 36 47 57 81 94 105 117 131 144 T89-32 26 32 38 49 58 83 98 107 120 134 148 T89-33 24 29 36 48 57 85 100 112 124 134 149 T89-34 21 26 32 43 53 79 90 102 117 131 148 T89-35 21 28 36 46 55 80 94 105 120 133 149 T89-36 16 21 28 39 51 74 88 100 115 128 144 T89-37 28 35 41 52 63 88 101 111 125 137 154 T89-38 27 32 39 51 62 86 101 113 128 140 155 T89-39 21 26 33 46 55 83 101 112 126 139 156 T89-40 21 26 33 45 55 77 88 99 113 124 139 T89-41 13 16 23 32 42 68 85 95 107 118 133 T89-42 24 30 37 49 61 86 101 113 128 143 150 T89-43 25 31 38 51 61 86 102 114 130 144 163 T89-44 23 31 39 51 64 90 105 118 135 151 169

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Days in the greenhouse 18 22 Height (cm) 53 57 25 29 32 39 43 46 50 T89-45 26 32 37 49 58 85 100 110 124 137 153 T89-46 20 25 34 43 55 81 97 109 122 133 149

Table 1.14b Growth data in diameter for TFSTT019

Days in the greenhouse 29 32 39 Diameter (mm) 43 46 50 53 57 TFSTT019-1A AT AT AT AT AT AT AT AT TFSTT019-1BA 3.7 5.0 7.1 7.9 8.4 8.6 9.7 10.2 TFSTT019-1BB 4.0 4.7 6.4 7.3 8.0 8.7 9.2 9.4 TFSTT019-2A 4.5 5.1 7.2 7.8 9.0 9.8 10.2 10.7 TFSTT019-2B 3.5 4.2 6.0 7.1 7.6 8.5 9.0 9.1 TFSTT019-3A 4.1 5.1 6.9 8.4 9.0 10.5 10.8 11.1 TFSTT019-4BA 4.4 5.5 7.3 8.4 9.2 9.5 10.1 11.3 TFSTT019-4BB 4.5 5.7 7.1 8.1 8.4 9.5 10.2 10.6 T89-01 3.2 3.7 5.3 6.2 6.9 7.9 8.3 8.7 T89-02 3.2 3.9 5.1 6.4 7.0 7.6 8.6 8.6 T89-03 4.2 5.3 6.4 6.9 6.9 AT 8.8 9.8 T89-04 2.2 3.0 4.2 4.5 5.4 5.0 5.4 6.1 T89-05 3.0 3.7 4.8 5.9 6.4 7.0 7.6 7.9 T89-06 3.6 4.8 6.5 7.6 8.7 9.1 9.6 10.1 T89-07 3.8 4.9 6.8 7.6 8.0 8.6 9.0 9.7 T89-08 4.4 5.8 6.2 7.3 7.6 8.5 9.4 9.7 T89-09 4.5 5.5 6.5 7.1 7.6 9.1 9.4 9.8 T89-10 3.7 3.9 5.0 6.1 6.8 7.6 8.5 9.2 T89-11 AT AT AT AT AT AT AT AT T89-12 4.6 5.5 7.3 8.6 8.8 9.6 10.2 10.2 T89-13 3.8 4.6 5.7 6.5 6.6 7.4 8.1 8.6 T89-14 3.7 4.2 5.3 5.6 6.1 7.0 7.1 7.8 T89-15 4.6 5.0 6.4 7.0 7.4 8.0 8.4 9.1 T89-16 5.0 5.5 7.0 7.6 8.0 8.6 9.0 9.7 T89-17 3.1 4.3 5.7 7.1 7.4 7.9 8.4 9.2 T89-18 4.5 5.3 7.8 8.0 8.1 9.2 9.9 10.3 T89-19 4.2 5.8 6.7 8.2 8.5 8.5 9.0 9.5

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T89-20 4.0 4.7 6.7 8.1 7.6 8.5 8.6 9.4 T89-21 3.5 3.9 5.0 5.7 5.8 7.1 6.7 7.4 T89-22 3.6 4.3 5.5 6.3 6.8 7.9 8.4 8.8 T89-23 4.1 5.2 7.0 7.6 8.2 8.9 9.2 10.0 T89-24 4.1 5.4 6.7 7.5 7.8 8.6 9.6 10.4 T89-25 3.7 4.6 6.0 7.0 7.7 8.3 8.5 9.4 T89-26 4.8 5.6 6.9 7.3 7.8 8.6 9.2 9.5 T89-27 3.8 4.9 6.2 6.7 6.9 7.6 8.4 9.3 T89-28 4.5 5.5 6.8 7.3 7.8 9.1 9.1 9.5 T89-29 4.4 5.3 6.8 6.8 7.4 8.2 9.2 8.9 T89-30 3.7 4.7 5.4 6.6 7.0 7.0 7.7 8.5 T89-31 4.0 4.5 5.5 6.9 7.0 8.0 9.1 9.6 T89-32 3.6 4.5 5.7 7.0 7.2 8.0 9.1 9.4 T89-33 3.9 4.6 6.7 7.3 8.0 8.6 9.4 10.5 T89-34 3.6 4.5 5.9 6.9 7.5 8.2 9.1 9.4 T89-35 3.9 4.5 5.7 7.1 7.5 7.9 8.8 9.6 T89-36 3.6 5.0 5.7 6.5 6.8 7.9 8.2 9.1 T89-37 4.3 5.6 7.1 8.0 8.0 8.9 9.7 10.2 T89-38 4.7 5.8 6.7 7.8 8.0 8.7 9.2 9.7 T89-39 4.2 5.0 6.2 7.3 8.2 8.1 8.7 9.6 T89-40 3.6 4.4 5.2 5.7 6.0 7.2 7.2 8.0 T89-41 3.5 4.3 5.5 6.5 7.0 7.5 7.7 8.4 T89-42 4.4 5.1 7.5 8.4 9.5 9.9 10.0 10.3 T89-43 4.3 5.0 6.5 7.3 7.8 8.3 8.9 9.1 T89-44 4.3 5.8 6.8 8.1 8.6 9.5 9.9 10.5 T89-45 4.2 4.9 6.8 7.5 7.6 8.5 9.4 9.7 T89-46 3.5 4.4 5.7 7 7.5 8.6 9.4 9.8

[00234] The results of the growth analysis are specified in general Table 1.14c. The determined growth effects of the specified construction group are presented as ratios between AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC of the construction group and the wild type group.

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Maximum growth rate in diameter 1.09 Maximum maximum growth rate 0.89 Maximum final diameter 1.08 Final maximum height 0.93 Average growth rate in diameter CO Average maximum growth rate 0.96 Final average diameter 1.11 Final average height 0.99 Construction group TFSTT019

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1.3.12 Construction group TFSTT035 [00235] This construction induces increased growth. The final diameter is 8% larger when compared to the average of the construction group and the control group of the wild type. The final diameter is 11% larger when comparing the larger individuals of the construction group and the control group of the wild type. The growth rate in diameter is 12% higher when comparing the average of the construction group and the control group of the wild type. The growth rate in diameter is 8% higher when comparing the largest individuals in the construction group and the wild type control group. Construction group TFSTT035 meets level (2) of the growth difference selection criterion, as shown in table 1.15c.

[00236] Tables 1.15a and 1.15b contain growth data for the specified construction group and the corresponding wild type group. The columns in the tables contain the height and diameter measurements of individuals from the specified construction group and the corresponding wild type group. The measurement time, in the form of number of days in the greenhouse, is shown in the table headings. Table 1.15a - Height growth data for TFSTT035

Height (cm)

Days in the greenhouse 18 22 25 29 32 39 43 46 50 53 57 TFSTT035-1A 23 30 36 49 60 88 101 112 127 141 158 TFSTT035-1BA 21 27 34 47 58 83 97 109 125 138 150 TFSTT035-1BB 23 28 35 47 56 80 94 105 118 129 143 TFSTT035-2AA 22 28 36 49 59 84 98 107 122 135 150 TFSTT035-2AB 20 25 31 41 49 70 83 95 109 121 137 TFSTT035-2B 18 23 29 40 51 75 90 100 112 125 138 TFSTT035-3B 20 26 31 42 52 77 93 103 116 129 145 TFSTT035-4B 14 19 25 35 44 73 88 98 113 127 141 T89-01 14 17 22 31 40 64 76 88 102 118 135 T89-02 17 20 25 36 45 69 79 90 104 118 133 T89-03 21 29 35 47 57 81 94 104 119 132 147 T89-04 13 14 17 25 33 45 52 57 70 79 92

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T89-05 15 18 24 33 42 69 83 94 106 120 137 T89-06 18 23 29 40 51 78 91 103 120 134 152 T89-07 20 24 30 41 51 74 88 96 109 121 130 T89-08 27 32 40 52 62 88 99 111 124 134 148 T89-09 23 27 34 46 56 83 97 106 122 132 149 T89-10 13 15 20 27 37 59 72 83 98 111 128 T89-11 AT AT AT AT AT AT AT AT AT AT AT T89-12 26 33 40 54 66 92 107 120 135 149 166 T89-13 21 26 34 45 55 79 94 105 122 134 150 T89-14 18 23 31 42 53 80 97 107 120 128 145 T89-15 26 33 40 53 63 88 103 111 125 138 154 T89-16 27 32 39 51 63 89 104 116 132 146 162 T89-17 17 21 27 38 45 72 87 97 110 124 139 T89-18 23 30 35 46 55 80 94 105 117 131 145 T89-19 23 29 35 45 54 78 91 102 114 125 137 T89-20 22 28 34 49 60 87 100 110 125 137 155 T89-21 27 31 35 42 48 66 77 87 100 110 124 T89-22 13 16 23 32 48 67 81 92 105 118 134 T89-23 23 29 35 48 58 83 97 107 122 136 154 T89-24 23 29 36 48 59 86 101 113 128 141 159 T89-25 16 21 28 40 51 80 97 107 122 137 153 T89-26 27 35 40 54 66 93 107 118 132 144 162 T89-27 26 31 38 49 59 82 96 106 120 131 146 T89-28 25 29 36 49 59 83 97 106 119 131 147 T89-29 26 32 38 51 62 88 103 113 128 142 159 T89-30 24 29 36 48 56 80 93 104 117 131 145 T89-31 24 31 36 47 57 81 94 105 117 131 144 T89-32 26 32 38 49 58 83 98 107 120 134 148 T89-33 24 29 36 48 57 85 100 112 124 134 149 T89-34 21 26 32 43 53 79 90 102 117 131 148 T89-35 21 28 36 46 55 80 94 105 120 133 149 T89-36 16 21 28 39 51 74 88 100 115 128 144 T89-37 28 35 41 52 63 88 101 111 125 137 154 T89-38 27 32 39 51 62 86 101 113 128 140 155 T89-39 21 26 33 46 55 83 101 112 126 139 156 T89-40 21 26 33 45 55 77 88 99 113 124 139 T89-41 13 16 23 32 42 68 85 95 107 118 133 T89-42 24 30 37 49 61 86 101 113 128 143 150 T89-43 25 31 38 51 61 86 102 114 130 144 163

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T89-44 23 31 39 51 64 90 105 118 135 151 169 T89-45 26 32 37 49 58 85 100 110 124 137 153 T89-46 20 25 34 43 55 81 97 109 122 133 149

Table 1.15b - TFSTT035 diameter growth data

Days in the greenhouse 29 32 Diameter (mm) 39 43 46 50 53 57 TFSTT035-1A 4.6 5.6 7.0 8.3 8.7 8.8 9.1 10.4 TFSTT035-1BA 4.5 5.7 7.3 8.8 9.0 10.0 11.0 11.7 TFSTT035-1BB 4.1 5.3 6.5 7.1 7.5 8.3 9.0 9.5 TFSTT035-2AA 4.1 5.0 7.0 8.0 8.7 9.2 10.0 10.3 TFSTT035-2AB 4.0 4.9 6.5 7.5 8.0 8.6 9.2 9.8 TFSTT035-2B 3.6 5.0 6.6 7.3 8.0 8.3 8.9 9.5 TFSTT035-3B 3.5 4.2 5.8 6.8 7.2 8.0 8.9 9.5 TFSTT035-4B 3.4 4.5 5.5 7.0 7.4 7.7 9.0 9.5 T89-01 3.2 3.7 5.3 6.2 6.9 7.9 8.3 8.7 T89-02 3.2 3.9 5.1 6.4 7.0 7.6 8.6 8.6 T89-03 4.2 5.3 6.4 6.9 6.9 AT 8.8 9.8 T89-04 2.2 3.0 4.2 4.5 5.4 5.0 5.4 6.1 T89-05 3.0 3.7 4.8 5.9 6.4 7.0 7.6 7.9 T89-06 3.6 4.8 6.5 7.6 8.7 9.1 9.6 10.1 T89-07 3.8 4.9 6.8 7.6 8.0 8.6 9.0 9.7 T89-08 4.4 5.8 6.2 7.3 7.6 8.5 9.4 9.7 T89-09 4.5 5.5 6.5 7.1 7.6 9.1 9.4 9.8 T89-10 3.7 3.9 5.0 6.1 6.8 7.6 8.5 9.2 T89-11 AT AT AT AT AT AT AT AT T89-12 4.6 5.5 7.3 8.6 8.8 9.6 10.2 10.2 T89-13 3.8 4.6 5.7 6.5 6.6 7.4 8.1 8.6 T89-14 3.7 4.2 5.3 5.6 6.1 7.0 7.1 7.8 T89-15 4.6 5.0 6.4 7.0 7.4 8.0 8.4 9.1 T89-16 5.0 5.5 7.0 7.6 8.0 8.6 9.0 9.7 T89-17 3.1 4.3 5.7 7.1 7.4 7.9 8.4 9.2 T89-18 4.5 5.3 7.8 8.0 8.1 9.2 9.9 10.3 T89-19 4.2 5.8 6.7 8.2 8.5 8.5 9.0 9.5 T89-20 4.0 4.7 6.7 8.1 7.6 8.5 8.6 9.4

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T89-21 3.5 3.9 5.0 5.7 5.8 7.1 6.7 7.4 T89-22 3.6 4.3 5.5 6.3 6.8 7.9 8.4 8.8 T89-23 4.1 5.2 7.0 7.6 8.2 8.9 9.2 10.0 T89-24 4.1 5.4 6.7 7.5 7.8 8.6 9.6 10.4 T89-25 3.7 4.6 6.0 7.0 7.7 8.3 8.5 9.4 T89-26 4.8 5.6 6.9 7.3 7.8 8.6 9.2 9.5 T89-27 3.8 4.9 6.2 6.7 6.9 7.6 8.4 9.3 T89-28 4.5 5.5 6.8 7.3 7.8 9.1 9.1 9.5 T89-29 4.4 5.3 6.8 6.8 7.4 8.2 9.2 8.9 T89-30 3.7 4.7 5.4 6.6 7.0 7.0 7.7 8.5 T89-31 4.0 4.5 5.5 6.9 7.0 8.0 9.1 9.6 T89-32 3.6 4.5 5.7 7.0 7.2 8.0 9.1 9.4 T89-33 3.9 4.6 6.7 7.3 8.0 8.6 9.4 10.5 T89-34 3.6 4.5 5.9 6.9 7.5 8.2 9.1 9.4 T89-35 3.9 4.5 5.7 7.1 7.5 7.9 8.8 9.6 T89-36 3.6 5.0 5.7 6.5 6.8 7.9 8.2 9.1 T89-37 4.3 5.6 7.1 8.0 8.0 8.9 9.7 10.2 T89-38 4.7 5.8 6.7 7.8 8.0 8.7 9.2 9.7 T89-39 4.2 5.0 6.2 7.3 8.2 8.1 8.7 9.6 T89-40 3.6 4.4 5.2 5.7 6.0 7.2 7.2 8.0 T89-41 3.5 4.3 5.5 6.5 7.0 7.5 7.7 8.4 T89-42 4.4 5.1 7.5 8.4 9.5 9.9 10.0 10.3 T89-43 4.3 5.0 6.5 7.3 7.8 8.3 8.9 9.1 T89-44 4.3 5.8 6.8 8.1 8.6 9.5 9.9 10.5 T89-45 4.2 4.9 6.8 7.5 7.6 8.5 9.4 9.7 T89-46 3.5 4.4 5.7 7 7.5 8.6 9.4 9.8

[00237] The results of the growth analysis are specified in general Table 1.15c. The determined growth effects of the specified construction group are presented as ratios between AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC of the construction group and the wild type group.

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Maximum growth rate in diameter 1.08 Maximum maximum growth rate 0.90 Maximum final diameter 1.11 Final maximum height 0.93 Average growth rate in diameter 1.12 Average maximum growth rate 0.98 Final average diameter 1.08 Final average height 0.99 Construction group TFSTT035

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1.3.13 Construction group TFSTT047 [00238] This construction induces increased growth. The final diameter is 8% larger when compared to the average of the construction group and the control group of the wild type. The final diameter is 11% larger when comparing the larger individuals of the construction group and the control group of the wild type. The growth rate in diameter is 12% higher when comparing the average of the construction group and the control group of the wild type. The growth rate in diameter is 8% higher when comparing the largest individuals in the construction group and the wild type control group. Construction group TFSTT047 meets the level of criterion (3) for selecting growth differences, as shown in Table 1.16c.

[00239] Tables 1.16a and 1.16b contain growth data for the specified construction group and the corresponding wild type group. The columns in the tables contain the height and diameter measurements of individuals from the specified construction group and the corresponding wild type group. The measurement time, in the form of number of days in the greenhouse, is shown in the table headings. Table 1.16a - Height growth data for TFSTT047

Height (cm)

Greenhouse days 20 28 34 37 41 48 51 55 59 TFSTT047-1B 29 44 56 64 73 91 99 111 121 TFSTT047-2A 29 47 67 76 87 114 122 135 147 TFSTT047-2B 26 44 67 76 90 116 125 139 150 TFSTT047-3B 25 43 65 74 87 109 118 131 143 TFSTT047-4A 25 45 68 75 87 108 115 128 138 T89-02 27 40 51 58 66 86 94 105 115 T89-03 27 44 58 67 77 97 104 114 126 T89-04 27 39 56 65 74 92 100 109 118 T89-07 28 47 66 77 88 107 116 130 140 T89-08 19 37 51 59 69 90 99 109 119

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T89-09 25 43 58 67 78 100 109 121 131 T89-11 29 45 60 69 81 104 111 121 132 T89-12 26 41 58 65 77 105 114 127 136 T89-13 31 47 70 78 90 110 119 131 143 T89-14 26 46 70 78 88 108 116 127 137 T89-18 22 41 55 65 74 95 104 115 126 T89-19 27 43 60 70 81 102 110 121 131 T89-20 25 40 57 AT 74 94 103 118 130 T89-21 27 45 61 72 84 108 115 126 137 T89-22 25 38 55 65 77 95 104 115 125 T89-23 23 37 50 57 65 82 92 106 115 T89-24 25 41 54 60 69 87 94 102 110 T89-25 25 38 55 66 80 100 109 121 133 T89-26 24 38 53 61 69 81 87 95 104 T89-27 26 39 54 62 70 90 97 109 120 T89-28 27 46 67 75 86 107 114 126 136 T89-29 24 39 57 64 74 91 98 108 128 T89-31 27 43 57 65 73 91 97 105 113 T89-32 25 41 55 63 75 96 107 121 135 T89-35 25 38 53 62 73 88 96 103 112 T89-36 27 45 67 78 90 113 122 134 145 T89-37 20 43 59 68 79 101 112 124 137 T89-39 25 45 61 69 79 99 108 120 132 T89-40 23 32 43 49 60 76 84 94 104 T89-41 27 45 66 75 87 112 120 133 144 T89-43 26 38 54 61 70 93 100 109 120 T89-46 26 45 66 76 88 109 114 125 133

Table 1.16b - Growth data in diameter for TFSTT047

Days in the greenhouse 34 41 Diameter (mm) 48 55 59 TFSTT047-1B 6.1 7.0 8.3 9.3 9.4 TFSTT047-2A 6.7 7.6 8.7 10.0 10.4 TFSTT047-2B 6.9 7.5 AT 9.5 10.6

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TFSTT047-3B 6.8 7.8 8.9 9.9 10.1 TFSTT047-4A 5.7 6.9 8.2 9.1 9.8 T89-02 5.6 6.4 7.8 9.0 8.4 T89-03 6.6 7.7 8.5 8.9 9.6 T89-04 7.2 7.8 9.1 9.8 9.7 T89-07 7.3 7.9 9.5 9.9 10.1 T89-08 5.5 7.8 8.3 10.0 9.8 T89-09 6.7 7.8 9.3 9.7 9.8 T89-11 6.4 8.1 9.2 9.4 9.7 T89-12 7.0 8.0 9.0 10.1 9.9 T89-13 6.8 8.5 9.1 10.7 10.8 T89-14 6.1 6.9 7.7 8.4 8.8 T89-18 5.5 6.5 7.9 9.5 9.4 T89-19 7.5 8.3 9.0 9.5 10.4 T89-20 7.3 7.1 8.7 9.3 9.7 T89-21 7.0 8.6 9.3 10.3 10.3 T89-22 7.0 7.8 8.4 10.0 9.5 T89-23 5.8 6.8 7.9 9.9 9.7 T89-24 6.1 6.8 8.1 9.0 9.4 T89-25 6.5 8.8 9.5 10.0 10.9 T89-26 5.8 6.7 7.0 7.5 7.7 T89-27 6.3 7.4 8.6 9.0 9.5 T89-28 7.0 8.1 8.8 9.7 10.5 T89-29 7.5 7.4 8.5 10.7 10.0 T89-31 5.6 6.3 7.2 7.6 8.6 T89-32 6.0 7.0 8.2 8.7 9.3 T89-35 5.4 6.4 6.8 7.9 8.0 T89-36 6.7 8.2 8.9 9.6 9.4 T89-37 7.4 8.5 8.6 9.3 10.8 T89-39 7.1 7.9 9.6 10.2 10.2 T89-40 3.9 5.2 5.7 6.8 6.6 T89-41 7.2 8.7 AT 9.7 10.3 T89-43 6.9 7.5 8.6 9.7 10.4

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Τ89-46 | 6.4 7.2 8.2 8.7 9.7 [00240] The results of the growth analysis are specified in general Table 1.16c. The determined growth effects of the specified construction group are presented as ratios between AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC of the construction group and the wild type group.

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Maximum growth rate in diameter 1.00 Maximum maximum growth rate 0.99 Maximum final diameter 0.97 Final maximum height 1.03 Average growth rate in diameter 1.20 Average maximum growth rate 1.08 Final average diameter 1.05 Final average height 1.10 Construction group TFSTT047

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Example 2

Construction group TF0002Rp2 [00241] This construction induces increased growth. The final height is 29% higher when comparing the average of the construction group and the control group of the wild type. The final height is 27% higher when comparing the largest individuals in the construction group and the control group in the wild type. The growth rate at maximum height is 36% higher when comparing the average of the construction group and the control group of the wild type. The growth rate at maximum height is 38% higher when comparing the largest individuals in the construction group and the control group in the wild type. The growth rate in diameter is 10% higher when comparing the average of the construction group and the control group of the wild type. The growth rate in diameter is 9% higher when comparing the largest individuals in the construction group and the wild type control group. Construction group TF0002Rp2 meets the most stringent levels of criteria (1), (3) and (4) for selecting growth differences, as shown in Table 2.3.

[00242] Tables 2.1 and 2.2 contain growth data for the specified construction group and the corresponding wild type group. The columns in the tables contain the height and diameter measurements of individuals from the specified construction group and the corresponding wild type group. The measurement time, in the form of number of days in the greenhouse, is shown in the table headings.

Table 2.1 Height growth data for TF0002Rp2

Days in the greenhouse 20 25 Height (cm) 57 60 29 35 41 46 50 53 TF0002rp2-1B-1 18 25 33 50 72 91 106 120 132 143 TF0002rp2-1 B-2 21 31 42 62 85 106 120 132 146 155 TF0002rp2-1 B-3 19 26 36 54 77 100 113 125 140 153

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Height (cm)

Days in the greenhouse 20 25 29 35 41 46 50 53 57 60 TF0002rp2-2A-1 19 28 38 52 72 91 103 115 127 138 TF0002rp2-2A-2 20 23 28 36 53 73 83 94 106 116 TF0002rp2-2A-3 22 31 40 57 73 94 101 109 116 124 TF0002rp2-3B-1 17 18 23 36 52 70 81 92 106 118 TF0002rp2-3B-2 19 28 37 56 78 103 115 125 139 150 TF0002rp2-3B-3 19 29 41 60 85 111 126 137 150 161 T89-01 17 26 31 45 61 76 87 95 107 116 T89-02 16 21 26 38 53 67 76 83 AT AT T89-03 18 24 32 47 64 78 87 96 106 115 T89-04 18 26 35 50 66 80 87 96 107 115 T89-05 17 26 33 44 57 73 81 91 100 107 T89-06 16 21 28 40 55 74 79 89 99 106 T89-07 17 23 31 43 57 71 80 88 98 107 T89-08 15 20 26 37 51 65 75 84 94 100 T89-09 18 25 32 46 61 74 85 93 103 112 T89-10 19 25 32 46 60 75 86 95 104 112 T89-11 20 27 36 51 68 86 98 107 120 127 T89-12 19 27 36 50 67 80 89 97 108 118 T89-13 18 25 32 45 59 75 83 92 101 108 T89-14 18 23 26 36 51 65 72 79 89 96 T89-15 17 21 28 39 53 70 78 87 97 105 T89-16 19 25 32 43 57 71 81 90 101 109 T89-17 16 20 29 41 54 68 78 84 93 101 T89-18 16 23 30 45 63 78 87 94 103 110 T89-19 16 22 28 42 56 69 79 89 100 107 T89-20 18 25 32 47 63 78 89 99 109 115 T89-21 19 27 34 50 67 82 92 102 111 120 T89-22 19 25 32 44 58 76 84 93 102 109 T89-23 18 26 33 47 63 79 88 99 108 116 T89-24 16 24 28 42 53 70 78 85 94 103 T89-25 16 25 32 45 61 76 85 95 107 114

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Days in the greenhouse 20 25 29 Height (cm) 35 41 46 50 53 57 60 T89-26 16 20 26 38 52 68 78 86 97 103 T89-27 16 21 25 35 48 61 70 78 AT 98 T89-28 14 18 25 38 51 64 73 81 90 96 T89-29 7 13 18 30 45 60 69 77 90 98 T89-30 15 22 29 42 55 69 80 88 97 106 T89-31 21 29 37 50 65 80 88 98 108 116 T89-32 19 24 32 42 55 70 78 87 96 105

Table 2.2 - Diameter growth data for TF0002Rp2

Days in the greenhouse 35 Diameter (mm) 60 Days in the greenhouse 35 Diameter (mm) 41 46 53 41 46 53 60 TF0002rp21B-1 4.6 5.4 6.3 8.0 8.2 T89-12 4.7 5.9 7.3 7.4 7.6 TF0002rp21B-2 4.5 5.4 6.5 7.7 8.7 T89-13 5.9 5.9 6.7 7.5 8.3 TF0002rp21B-3 4.9 5.9 6.4 7.2 8.0 T89-14 4.5 5.2 5.8 6.1 7.0 TF0002rp22A-1 4.3 4.9 6.0 7.4 8.0 T89-15 4.7 AT 6.0 6.4 7.3 TF0002rp22A-2 4.1 5.3 6.3 6.5 7.6 T89-16 4.5 5.6 7.7 7.5 8.6 TF0002rp22A-3 4.7 6.3 6.2 7.7 9.8 T89-17 4.1 5.4 6.0 6.8 7.5 TF0002rp23B-1 3.4 4.4 5.1 6.9 7.1 T89-18 4.4 5.8 6.0 7.7 8.3 TF0002rp23B-2 4.9 6.5 6.8 8.5 9.3 T89-19 4.7 5.8 7.1 8.5 9.4 TF0002rp23B-3 4.7 6.3 6.9 8.5 8.7 T89-20 4.7 5.8 6.6 7.4 8.3 T89-01 4.7 6.0 6.5 6.9 8.3 T89-21 4.7 6.2 6.3 7.7 8.5 T89-02 4.4 5.7 6.6 7.6 7.8 T89-22 4.2 5.1 6.3 6.9 8.0 T89-03 4.7 6.3 6.7 7.7 8.4 T89-23 4.6 5.8 7.1 7.5 8.3 T89-04 4.7 6.0 7.1 8.0 8.5 T89-24 4.4 5.8 6.9 7.7 8.8 T89-05 4.0 5.3 6.3 7.8 8.1 T89-25 4.5 5.4 6.2 8.0 8.7 T89-06 4.6 5.9 6.3 7.7 8.2 T89-26 4.1 5.4 6.0 7.2 8.0 T89-07 4.6 6.1 6.5 7.9 8.5 T89-27 4.5 5.7 6.5 7.9 9.2

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Days in the greenhouse Diameter (mm) Days in the greenhouse Diameter (mm) 35 41 46 53 60 35 41 46 53 60 T89-08 4.0 5.2 5.8 6.9 7.1 T89-28 4.3 5.1 6.0 6.6 7.7 T89-09 4.4 5.8 6.4 7.6 8.2 T89-29 3.5 4.7 5.5 6.3 7.1 T89-09 4.4 5.8 6.4 7.6 8.2 T89-30 4.1 5.4 5.8 7.2 7.8 T89-10 4.9 6.0 6.6 7.4 8.5 T89-31 5.0 5.8 6.8 7.3 8.2 T89-11 4.7 5.8 6.4 7.9 8.3 T89-32 4.4 6.4 6.6 8.0 8.4

[00243] The results of the growth analysis are specified in general Table 2.3. The determined growth effects of the specified construction group are presented as ratios between AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC of the construction group and the wild type group.

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Maximum growth rate in diameter 1.09 Maximum growth rate at maximum height 1.38 Maximum final diameter 1.05 Final maximum height 1.27 Average growth rate in diameter 1.10 Maximum growth rate at average height 1.36 Final average diameter 1.03 Final average height 1.29 Construction group TF0002Rp2

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Construction group TF0003 [00244] Tables 2.4 and 2.5 contain growth data for the specified construction group and the corresponding wild type group. The columns in the tables contain the height and diameter measurements of individuals from the specified construction group and the corresponding wild type group. The measurement time, in the form of number of days in the greenhouse, is shown in the table headings. Table 2.4 Height growth data for TF0003

Height (cm)

Days in the greenhouse 18 25 35 39 46 49 53 TF0003-1A 32 55 94 113 145 156 168 TF0003-1B 37 58 104 120 143 155 170 TF0003-2A 30 49 86 107 142 159 178 TF0003-3A 38 59 100 114 142 152 167 TF0003-3B 29 51 98 117 147 162 183 TF0003-4A 30 52 97 116 149 162 176 TF0003-4B 32 52 91 112 146 162 179 T89-01 33 57 108 128 161 173 192 T89-02 32 55 94 112 145 160 180 T89-03 31 53 95 114 147 160 173 T89-04 27 48 91 110 143 157 179 T89-05 26 43 86 107 141 155 171 T89-06 28 47 86 106 142 157 175 T89-07 34 56 101 120 153 168 184 T89-08 26 44 83 101 133 148 166 T89-09 29 51 88 106 141 154 171 T89-10 25 45 90 108 141 156 178 T89-11 26 46 80 96 128 143 166 T89-12 27 45 84 102 132 143 156 T89-13 29 50 96 115 149 163 185 T89-14 28 49 89 109 147 163 185 T89-15 25 43 81 99 134 146 161 T89-16 27 46 88 106 146 162 185 T89-17 31 52 90 107 142 158 179 T89-18 28 45 82 101 140 158 180 T89-19 27 42 82 101 132 146 163 T89-20 28 42 81 101 135 150 169

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T89-21 26 36 67 76 100 113 128 T89-22 31 51 97 115 150 165 181 T89-23 29 49 83 101 132 144 163 T89-24 30 48 89 107 144 159 177 T89-25 26 45 89 103 136 152 175 T89-26 28 48 87 105 140 156 177 T89-27 28 46 86 103 138 153 170 T89-28 29 47 86 103 135 152 173 T89-29 26 43 81 101 139 156 179 T89-30 32 53 94 114 150 166 187 T89-31 30 48 85 103 134 148 169 T89-32 31 51 91 109 140 154 173 T89-33 31 52 94 113 151 167 186 T89-34 23 40 84 105 140 153 171 T89-35 29 48 88 106 137 150 165 T89-36 29 49 88 107 143 159 176 T89-37 32 49 91 105 141 154 168 T89-38 29 50 88 106 144 158 172 T89-39 31 48 89 106 139 152 172 T89-40 32 49 97 117 149 163 185 T89-41 24 46 86 104 138 155 176 T89-42 26 41 80 98 133 151 172 T89-43 29 48 92 110 145 163 188 T89-44 28 49 89 109 146 163 186 T89-45 28 48 83 97 119 129 145 T89-46 32 52 95 115 149 165 185 T89-47 26 42 83 101 133 147 166 T89-48 26 41 82 101 135 152 173 T89-49 33 52 103 123 153 166 180

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Table 2.5 Growth data in diameter for TF0003

Diameter (mm)

Days in the greenhouse 35 39 46 53 TF0003-1A 5.3 6.7 7.5 8.8 TF0003-1B 6.0 6.6 7.3 7.7 TF0003-2A 6.3 6.9 8.8 9.7 TF0003-3A 6.3 7.1 7.9 9.3 TF0003-3B 6.0 6.9 8.5 9.2 TF0003-4A 6.6 7.5 7.9 9.0 TF0003-4B 6.0 7.0 8.6 10.4 T89-01 7.2 8.0 9.6 10.7 T89-02 6.2 7.1 8.0 9.1 T89-03 6.3 7.3 8.3 9.3 T89-04 5.5 6.7 8.0 9.0 T89-05 5.3 6.5 7.4 8.3 T89-06 5.8 6.8 8.0 9.1 T89-07 6.3 7.3 9.1 10.9 T89-08 5.7 6.3 7.1 7.6 T89-09 6.0 6.6 8.1 9.1 T89-10 5.6 6.5 7.7 9.1 T89-11 5.8 6.6 7.7 8.6 T89-12 6.2 6.8 7.3 8.1 T89-13 5.7 6.6 7.7 8.8 T89-14 6.3 7.0 8.5 10.0 T89-15 5.2 6.0 6.9 7.6 T89-16 6.3 7.2 8.4 9.3 T89-17 5.9 6.8 8.0 9.1 T89-18 6.2 6.8 8.2 9.6 T89-19 6.1 6.8 7.9 9.1 T89-20 5.3 6.3 7.4 9.0 T89-21 3.5 4.2 5.0 9.2 T89-22 5.9 6.6 7.7 8.4 T89-23 6.1 7.0 8.4 9.7

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T89-24 5.7 6.7 8.0 9.4 T89-25 5.9 6.8 8.1 8.7 T89-26 6.4 7.2 8.4 9.7 T89-27 5.7 6.9 8.8 10.1 T89-28 5.7 6.6 7.7 8.7 T89-29 5.1 6.4 7.7 9.1 T89-30 6.0 6.9 8.1 10.1 T89-31 5.8 6.7 8.2 9.3 T89-32 6.2 6.9 7.8 8.8 T89-33 6.3 7.1 8.5 10.2 T89-34 5.8 6.8 8.1 9.8 T89-35 6.1 7.2 7.7 8.9 T89-36 6.3 6.9 8.7 10.0 T89-37 5.9 7.0 8.4 9.2 T89-38 6.0 6.6 7.5 8.2 T89-39 7.1 7.6 8.4 9.7 T89-40 6.1 7.0 8.3 9.7 T89-41 5.5 6.4 6.9 7.9 T89-42 5.4 6.2 7.8 9.1 T89-43 6.0 6.9 8.3 9.5 T89-44 5.9 7.2 9.0 10.5 T89-45 5.0 5.7 6.2 7.8 T89-46 6.2 7.5 8.6 9.8 T89-47 5.3 6.1 7.8 8.8 T89-48 5.4 6.2 7.6 8.2 T89-49 7.0 8.2 9.4 10.3

[00245] The results of the growth analysis are specified in general Table 2.6. The determined growth effects of the specified construction group are presented as ratios between AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC of the construction group and the wild type group.

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Maximum growth rate in diameter 0.73 Maximum growth rate at maximum height 0.91 Maximum final diameter 0.96 Final maximum height 0.95 Average growth rate in diameter 0.93 Maximum growth rate at average height 0.93 Final average diameter 1.00 Final average height 1.00 Construction group TF0003

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Construction group TF0011 [00246] Tables 2.7 and 2.8 contain growth data for the specified construction group and the corresponding wild type group. The columns in the tables contain the height and diameter measurements of individuals from the specified construction group and the corresponding wild type group. The measurement time, in the form of number of days in the greenhouse, is shown in the table headings.

Table 2.7 Height growth data for TF0011

Days in the greenhouse 19 25 32 Height (cm) 39 48 54 56 TF0011-1A-1 17 28 48 73 107 133 141 TF0011-1A-2 23 35 58 80 101 114 118 TF0011-1B 17 27 45 66 94 111 117 TF0011-2A-1 23 32 56 81 110 125 131 TF0011-2A-2 18 33 55 76 105 127 134 TF0011-3A-1 19 31 53 75 105 125 131 TF0011-3A-2 17 27 47 67 95 116 121 TF0011-3B-1 17 29 47 72 100 121 127 TF0011-3B-2 14 24 45 67 101 123 130 TF0011-4A 19 33 57 81 114 135 143 T89-01 18 28 49 70 100 126 133 T89-02 19 29 51 76 105 128 136 T89-03 11 18 36 47 76 93 98 T89-04 16 25 48 74 102 126 133 T89-05 17 29 49 73 106 128 135 T89-06 16 28 51 74 105 127 132 T89-07 19 28 51 73 104 125 136 T89-08 19 31 52 77 110 130 137 T89-09 17 26 44 67 93 114 122 T89-10 16 25 44 63 89 108 115 T89-11 20 31 47 68 87 114 118 T89-12 17 28 48 70 101 117 124

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T89-13 18 30 52 73 105 125 130 T89-14 19 29 44 67 96 116 123 T89-15 17 29 49 72 102 124 131 T89-16 18 29 50 74 108 129 135 T89-17 14 23 43 65 93 116 123 T89-18 15 26 48 72 103 128 136 T89-19 11 19 38 63 93 115 122 T89-20 19 30 52 75 104 126 133 T89-21 17 28 48 72 104 128 135 T89-22 19 29 49 73 105 123 129 T89-23 21 30 54 79 112 135 138 T89-24 19 24 37 54 77 100 103 T89-25 15 27 46 69 98 117 123 T89-26 19 29 50 73 103 127 135 T89-27 15 27 47 70 106 129 135 T89-28 20 33 55 80 109 130 137 T89-29 24 38 62 88 122 143 151 T89-30 21 33 56 78 113 132 138 T89-31 21 31 52 76 105 126 133 T89-32 18 29 50 73 105 129 138 T89-33 23 37 61 89 121 143 150 T89-34 19 30 52 78 108 130 138 T89-35 19 31 55 79 111 134 142 T89-36 18 29 52 75 105 127 134 T89-37 22 32 52 73 103 124 130 T89-38 18 29 51 74 105 128 134 T89-39 21 31 51 72 102 119 124 T89-40 20 32 51 74 99 121 128 T89-41 19 29 50 73 108 130 135 T89-42 15 26 46 68 95 113 119 T89-43 16 28 48 67 93 113 119 T89-44 21 31 50 70 97 116 122 T89-45 16 26 48 72 101 123 131

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T89-46 21 29 50 75 106 129 138 T89-47 9 16 34 55 82 106 114 T89-48 5 8 17 24 42 52 56 T89-49 20 30 50 71 96 117 125 T89-50 16 28 50 74 105 128 136 T89-51 19 29 51 75 107 128 136 T89-52 20 29 50 75 104 124 131 T89-53 21 32 50 72 101 118 125 T89-54 21 32 55 81 112 133 142 T89-55 21 32 56 83 112 133 141

Table 2.8 - Diameter growth data for TF0011

Days in the greenhouse Diameter (mm) Days in the greenhouse Diameter (mm) 39 48 54 56 39 48 54 56 TF0011-1A-1 5.7 6.9 7.6 7.9 T89-24 3.1 4.6 5.0 4.5 TF0011-1A-2 5.0 5.6 5.6 6.0 T89-25 5.9 7.7 8.5 8.9 TF0011-1B 5.2 7.1 7.7 7.6 T89-26 6.2 7.8 8.6 9.5 TF0011-2A-1 5.8 7.9 8.1 8.0 T89-27 6.1 7.6 8.2 8.6 TF0011-2A-2 6.2 7.9 9.2 9.3 T89-28 6.9 9.0 10.7 10.5 TF0011-3A-1 6.0 8.4 8.4 8.8 T89-29 7.8 8.4 10.3 10.2 TF0011-3A-2 6.0 7.5 8.0 8.5 T89-30 6.4 8.5 9.4 9.9 TF0011-3B-1 6.3 8.5 9.5 9.7 T89-31 6.8 8.6 8.8 9.5 TF0011-3B-2 7.7 8.4 9.3 9.5 T89-32 6.7 8.4 10.2 10.9 TF0011-4A 6.7 8.1 9.2 9.6 T89-33 7.1 8.7 9.4 11.4 T89-01 6.6 8.4 8.5 9.2 T89-34 7.1 8.5 11.1 10.9 T89-02 6.8 8.3 9.4 10.1 T89-35 6.7 9.0 8.9 11.4 T89-03 4.6 4.9 6.1 6.1 T89-36 6.2 8.0 8.5 8.5 T89-04 6.9 8.7 10.0 9.4 T89-37 5.6 7.1 7.8 8.7 T89-05 5.6 7.6 8.2 8.8 T89-38 5.8 7.8 8.8 8.7 T89-06 6.9 8.2 9.6 9.3 T89-39 6.2 8.1 9.2 9.3 T89-07 6.6 7.9 9.5 9.5 T89-40 6.2 7.9 9.6 9.5 T89-08 6.9 8.9 9.6 9.8 T89-41 5.9 8.2 8.9 9.5 T89-09 6.1 7.6 8.6 8.7 T89-42 6.8 8.4 9.1 10.4 T89-10 5.5 7.5 8.6 8.6 T89-43 6.7 8.5 9.7 9.3 T89-11 7.4 8.4 9.3 8.7 T89-44 7.1 9.1 9.2 9.4 T89-12 5.9 7.4 8.7 9.5 T89-45 6.6 7.8 9.2 9.2 T89-13 6.2 7.7 9.2 9.5 T89-46 6.8 9.3 10.2 10.0 T89-14 5.9 7.8 9.2 8.7 T89-47 5.3 7.0 7.9 8.4

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T89-15 6.8 8.1 9.4 9.7 T89-48 2.3 3.0 3.2 3.5 T89-16 7.3 9.2 10.6 10.3 T89-49 7.5 8.7 9.1 9.8 T89-17 6.4 8.0 8.9 9.1 T89-50 6.8 8.6 9.3 8.9 T89-18 7.0 7.5 8.1 8.8 T89-51 7.2 8.3 9.2 9.1 T89-19 5.6 7.4 8.6 8.9 T89-52 7.5 8.8 9.4 9.7 T89-20 6.5 8.3 8.6 9.5 T89-53 7.3 9.3 9.5 10.2 T89-21 6.2 8.1 8.0 8.7 T89-54 7.0 9.0 9.2 9.6 T89-22 6.2 8.5 9.3 10.1 T89-55 7.0 8.9 9.2 9.8 T89-23 6.2 8.1 9.2 9.5

[00247] The results of the growth analysis are specified in Table 2.9 general of the effects. The determined growth effects of the specified construction group are presented as ratios between AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC of the construction group and the wild type group.

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Maximum growth rate in diameter O Maximum growth rate at maximum height 1.04 Maximum final diameter 0.85 Final maximum height 0.95 Average growth rate in diameter co co o Maximum growth rate at average height 1.00 Final average diameter 0.92 Final average height 1.00 Construction group o o LL H

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Construction group TF0013rp2 [00248] Tables 2.10 and 2.11 contain growth data for the specified construction group and the corresponding wild type group. The columns in the tables contain the height and diameter measurements of individuals from the specified construction group and the corresponding wild type group. The measurement time, in the form of number of days in the greenhouse, is shown in the table headings. Table 2.10 Height growth data for TF0013rp2

Height (cm)

Days in the greenhouse 15 19 26 33 37 40 47 51 54 TF0013rp2-1A-1 20 25 39 48 59 68 89 100 111 TF0013rp2-1A-2 18 22 34 52 64 74 101 115 131 TF0013rp2-1A-3 21 25 38 54 67 75 104 116 133 TF0013rp2-3BA1 TF0013rp2-3BA- 2 21 26 39 57 73 84 113 125 137 20 23 34 52 65 75 101 113 128 TF0013rp2-3BA- 20 24 36 55 71 83 115 131 145 T89-20 21 26 39 58 73 82 111 130 134 T89-21 21 24 34 51 64 72 97 103 125 T89-22 19 23 32 48 60 70 96 111 123 T89-23 21 24 34 51 63 70 94 112 122 T89-24 19 22 30 46 59 68 90 103 115 T89-25 22 30 39 57 72 81 106 112 130 T89-26 21 24 35 48 60 70 98 111 130 T89-27 21 24 34 48 60 70 93 106 120 T89-28 20 22 32 48 60 70 93 105 116

Table 2.11 Diameter growth data for TF0013rp2

Days in the greenhouse

TF0013rp2-1A-1

TF0013rp2-1A-2

Diameter (mm)

Days in the greenhouse 33 40 47 54 T89-21 3.8 5.5 6.0 6.9 T89-22 4.5 6.2 6.6 7.5

Diameter (mm)

40 47 54

3.8 5.0 6.3 6.8

5.4 5.7 7.3 8.1

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TF0013rp2-1A-3 4.4 5.4 6.6 7.6 T89-23 3.8 4.8 5.6 6.2 TF0013rp2-3BA-1 5.0 6.3 7.1 8.5 T89-24 3.8 4.9 5.8 7.2 TF0013rp2-3BA-2 4.2 5.4 7.0 8.5 T89-25 4.8 5.9 7.4 8.9 TF0013rp2-3BA-3 5.0 6.5 7.3 8.1 T89-26 4.4 6.2 7.0 7.8 T89-20 4.8 6.2 6.3 6.9 T89-27 4.9 6.4 7.3 8.3 T89-21 3.8 5.5 6.0 6.9 T89-28 4.5 4.8 5.8 7.2 T89-22 4.5 6.2 6.6 7.5

[00249] The results of the growth analysis are specified in general Table 2.12. The determined growth effects of the specified construction group are presented as ratios between AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC of the construction group and the wild type group.

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Maximum growth rate in diameter 0.96 Maximum growth rate at maximum height 1.06 Maximum final diameter 0.91 Final maximum height 1.08 Average growth rate in diameter 1.06 Maximum growth rate at average height 1.08 Final average diameter 1.03 Final average height 1.04 Construction group TF0013rp2

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Construction group TF0045 [00250] This construction induces increased growth. The final height is 6% higher when comparing the average of the construction group and the control group of the wild type. The final height is 11% higher when comparing the largest individuals in the construction group and the control group in the wild type. The growth rate at maximum height is 9% higher when comparing the average of the construction group and the control group of the wild type. The growth rate at maximum height is 12% higher when comparing the largest individuals in the construction group and the wild type control group. The final diameter is 10% larger when comparing the average of the construction group and the control group of the wild type. The final diameter is 9% larger when comparing the largest individuals in the construction group and the control group in the wild type. Construction group TF0045 meets the levels of the growth difference selection criterion (1), as shown in Table 2.15.

[00251] Tables 2.13 and 2.14 contain growth data for the specified construction group and the corresponding wild type group. The columns in the tables contain the height and diameter measurements of individuals from the specified construction group and the corresponding wild type group. The measurement time, in the form of number of days in the greenhouse, is shown in the table headings. Table 2.13 Height growth data for TF0045

Height (cm)

Days in the greenhouse 18 25 28 32 36 39 46 53 55 TF0045-1A-1 25 39 53 66 79 87 106 125 128 TF0045-1A-2 15 25 34 44 57 66 89 114 121 TF0045-1A-3 18 34 45 55 68 79 104 133 141 TF0045-1B-1 24 43 57 68 84 95 126 150 156 TF0045-1B-2 24 43 57 70 85 95 120 147 154

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Height (cm)

Days in the greenhouse 18 25 28 32 36 39 46 53 55 TF0045-1B-3 25 30 34 43 54 64 84 107 115 TF0045-2B-1 21 33 45 53 64 76 103 128 136 TF0045-2B-3 19 32 42 51 64 72 96 120 128 T89-01 18 32 43 54 66 76 98 124 133 T89-02 20 33 43 53 67 76 101 124 129 T89-03 17 31 43 53 68 76 102 128 135 T89-04 19 33 44 54 67 74 97 122 129 T89-05 20 33 44 54 67 76 100 121 129 T89-06 18 31 42 53 67 76 97 121 128 T89-07 16 28 41 53 65 74 96 121 129 T89-08 19 34 43 53 65 75 98 123 131 T89-09 20 30 40 52 64 73 97 119 125 T89-10 21 33 41 49 61 70 92 114 121 T89-11 18 31 40 49 61 71 94 117 123 T89-12 18 31 40 AT 60 68 90 107 115 T89-13 19 35 47 58 70 78 103 128 135 T89-14 19 31 40 50 61 72 93 118 124 T89-15 20 32 41 50 62 70 91 114 120 T89-16 18 30 39 49 62 71 92 114 122 T89-17 17 33 45 55 70 78 104 129 136 T89-18 19 32 44 56 71 82 106 130 137 T89-19 16 26 36 46 51 69 89 112 119 T89-20 16 31 41 49 60 71 91 111 118 T89-21 20 31 44 54 68 76 99 122 127 T89-22 14 26 37 46 61 72 96 120 127 T89-23 19 32 40 50 63 73 100 121 127 T89-24 20 31 41 47 58 68 90 111 117 T89-25 20 36 45 57 69 78 101 127 134 T89-26 20 37 49 58 71 80 107 131 140 T89-27 19 34 44 55 71 81 107 131 138 T89-28 17 35 44 56 68 76 99 126 132

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Height (cm)

Days in the greenhouse 18 25 28 32 36 39 46 53 55 T89-29 17 32 45 55 68 78 101 125 132 T89-30 18 31 41 50 63 72 95 119 125 T89-31 17 27 35 45 58 67 87 108 116 T89-32 19 32 44 52 65 74 98 121 127 T89-33 20 33 43 53 64 74 96 119 126 T89-34 19 34 44 55 68 78 100 124 130 T89-35 17 30 40 51 64 74 95 116 122 T89-36 18 30 40 49 62 71 91 114 121 T89-82 21 33 43 54 65 75 98 118 125 T89-83 22 35 47 55 71 80 105 130 137 T89-85 21 36 46 56 67 76 98 123 131

Table 2.14 Diameter growth data for TF0045

Days in the greenhouse 32 39 Diameter (mm) 46 53 55 TF0045-1A-1 5.8 6.5 6.9 7.5 7.6 TF0045-1A-2 4.3 6.8 8.4 8.7 9.2 TF0045-1A-3 5.6 6.7 7.9 9.2 9.2 TF0045-1B-1 5.9 7.1 8.8 9.7 10.0 TF0045-1B-2 6.8 7.8 8.4 10.1 9.9 TF0045-1B-3 4.3 4.4 4.6 5.9 5.9 TF0045-2B-1 5.3 7.1 7.4 8.5 8.8 TF0045-2B-3 5.4 6.2 8.0 8.9 8.8 T89-01 5.2 6.0 6.8 8.2 7.6 T89-02 4.5 5.8 7.1 8.6 8.3 T89-03 4.4 5.7 6.8 7.9 8.1 T89-04 4.4 6.0 6.9 8.6 8.5 T89-05 4.6 5.8 6.7 7.9 8.0 T89-06 4.7 5.9 7.2 8.1 9.2 T89-07 4.8 5.5 6.2 6.9 7.1 T89-08 4.5 5.4 6.0 6.9 7.1 T89-09 4.7 5.6 6.7 8.8 8.0

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Days in the greenhouse 32 39 Diameter (mm) 46 53 55 T89-10 4.1 4.9 5.6 7.1 7.0 T89-11 3.8 5.3 7.3 8.0 7.6 T89-12 AT 5.1 6.2 7.3 6.9 T89-13 4.8 5.7 6.5 7.6 7.6 T89-14 4.2 5.5 6.6 7.6 6.5 T89-15 5.0 6.1 7.2 7.9 8.3 T89-16 4.4 5.6 6.2 7.3 7.9 T89-17 4.8 6.7 7.0 8.3 8.4 T89-18 4.2 5.5 6.7 7.3 7.4 T89-19 4.7 5.5 6.8 7.2 7.8 T89-20 4.5 5.6 6.5 7.0 7.3 T89-21 4.9 5.6 6.4 7.4 7.6 T89-22 4.3 5.4 6.5 7.3 7.6 T89-23 3.9 5.5 6.7 7.7 7.0 T89-24 4.3 5.9 6.8 8.1 7.9 T89-25 4.7 6.5 7.5 9.2 9.1 T89-26 5.4 5.9 7.7 8.6 8.8 T89-27 4.7 5.6 7.4 7.9 8.0 T89-28 4.7 5.7 6.3 7.2 7.4 T89-29 4.8 5.6 6.6 7.7 8.1 T89-30 4.6 5.5 6.7 7.3 7.3 T89-31 4.3 5.8 6.3 7.5 7.6 T89-32 4.6 5.9 7.6 9.3 9.1 T89-33 4.4 5.3 6.2 7.1 7.1 T89-34 4.6 5.6 6.8 8.1 8.7 T89-35 5.3 6.0 7.5 8.8 8.9 T89-36 4.4 6.1 6.9 8.1 8.6 T89-82 4.7 5.3 6.5 7.5 7.4 T89-83 4.7 6.1 7.1 8.3 8.3 T89-85 5.1 6.3 7.3 7.7 8.2

[00252] The results of the growth analysis are specified

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164/362 in general Table 2.15. The determined growth effects of the specified construction group are presented as ratios between AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC of the construction group and the wild type group.

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Maximum growth rate in diameter 0.94 Maximum growth rate at maximum height 1.12 Maximum final diameter 1.09 Final maximum height 1.11 Average growth rate in diameter 0.98 Maximum growth rate at average height 1.09 Final average diameter 1.10 Final average height 1.06 Construction group TF0045

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Construction group TF0052Rp1 [00253] Tables 2.16 and 2.17 contain growth data for the specified construction group and the corresponding wild type group. The columns in the tables contain the height and diameter measurements of individuals from the specified construction group and the corresponding wild type group. The measurement time, in the form of number of days in the greenhouse, is shown in the table headings. Table 2.16 Height growth data for TF0052Rp1

Height

Days in the greenhouse 20 25 29 35 41 46 50 53 57 60 TF0052rp1-2A-1 21 29 38 53 74 90 103 115 127 138 TF0052rp1-2A-2 19 28 37 52 68 83 97 105 116 126 TF0052rp1-2A-3 15 23 33 50 69 85 97 108 117 129 TF0052rp1-2B-1 18 26 32 47 59 75 82 91 100 109 TF0052rp1-2B-2 18 23 27 35 49 67 75 84 94 101 TF0052rp1-2B-3 18 21 28 38 50 63 68 75 83 89 T89-01 17 26 31 45 61 76 87 95 107 116 T89-02 16 21 26 38 53 67 76 83 AT AT T89-03 18 24 32 47 64 78 87 96 106 115 T89-04 18 26 35 50 66 80 87 96 107 115 T89-05 17 26 33 44 57 73 81 91 100 107 T89-06 16 21 28 40 55 74 79 89 99 106 T89-07 17 23 31 43 57 71 80 88 98 107 T89-08 15 20 26 37 51 65 75 84 94 100 T89-09 18 25 32 46 61 74 85 93 103 112 T89-10 19 25 32 46 60 75 86 95 104 112 T89-11 20 27 36 51 68 86 98 107 120 127 T89-12 19 27 36 50 67 80 89 97 108 118 T89-13 18 25 32 45 59 75 83 92 101 108 T89-14 18 23 26 36 51 65 72 79 89 96 T89-15 17 21 28 39 53 70 78 87 97 105 T89-16 19 25 32 43 57 71 81 90 101 109

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Height

Days in the greenhouse 20 25 29 35 41 46 50 53 57 60 T89-17 16 20 29 41 54 68 78 84 93 101 T89-18 16 23 30 45 63 78 87 94 103 110 T89-19 16 22 28 42 56 69 79 89 100 107 T89-20 18 25 32 47 63 78 89 99 109 115 T89-21 19 27 34 50 67 82 92 102 111 120 T89-22 19 25 32 44 58 76 84 93 102 109 T89-23 18 26 33 47 63 79 88 99 108 116 T89-24 16 24 28 42 53 70 78 85 94 103 T89-25 16 25 32 45 61 76 85 95 107 114 T89-26 16 20 26 38 52 68 78 86 97 103 T89-27 16 21 25 35 48 61 70 78 AT 98 T89-28 14 18 25 38 51 64 73 81 90 96 T89-29 7 13 18 30 45 60 69 77 90 98 T89-30 15 22 29 42 55 69 80 88 97 106 T89-31 21 29 37 50 65 80 88 98 108 116 T89-32 19 24 32 42 55 70 78 87 96 105

Table 2.17 Diameter growth data for TF0052Rp1

Days in the greenhouse 35 Diameter (mm) 41 46 53 60 Days in the greenhouse 35 Diameter (mm) 41 46 53 60 TF0052rp1-2A-1 5.4 6.5 7.2 8.6 9.4 T89-14 4.5 5.2 5.8 6.1 7.0 TF0052rp1-2A-2 4.9 6.6 6.9 7.3 8.3 T89-15 4.7 AT 6.0 6.4 7.3 TF0052rp1-2A-3 5.3 6.7 7.5 8.4 9.5 T89-16 4.5 5.6 7.7 7.5 8.6 TF0052rp1-2B-1 5.0 6.2 6.9 9.0 8.0 T89-17 4.1 5.4 6.0 6.8 7.5 TF0052rp1-2B-2 4.9 5.9 6.6 6.8 8.1 T89-18 4.4 5.8 6.0 7.7 8.3 TF0052rp1-2B-3 3.3 4.2 4.4 5.1 5.7 T89-19 4.7 5.8 7.1 8.5 9.4 T89-01 4.7 6.0 6.5 6.9 8.3 T89-20 4.7 5.8 6.6 7.4 8.3 T89-02 4.4 5.7 6.6 7.6 7.8 T89-21 4.7 6.2 6.3 7.7 8.5 T89-03 4.7 6.3 6.7 7.7 8.4 T89-22 4.2 5.1 6.3 6.9 8.0 T89-04 4.7 6.0 7.1 8.0 8.5 T89-23 4.6 5.8 7.1 7.5 8.3 T89-05 4.0 5.3 6.3 7.8 8.1 T89-24 4.4 5.8 6.9 7.7 8.8 T89-06 4.6 5.9 6.3 7.7 8.2 T89-25 4.5 5.4 6.2 8.0 8.7 T89-07 4.6 6.1 6.5 7.9 8.5 T89-26 4.1 5.4 6.0 7.2 8.0 T89-08 4.0 5.2 5.8 6.9 7.1 T89-27 4.5 5.7 6.5 7.9 9.2

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Τ89-09

Τ89-10 Τ89-11

Τ89-12

-1389-13

4.4 5.8 6.4 7.6

4.9 6.0 6.6 7.4

4.7 5.8 6.4 7.9

4.7 5.9 7.3 7.4

5.9 5.9 6.7 7.5

8.2 T89-28 4.3 8.5 T89-29 3.5 8.3 T89-30 4.1 7.6 T89-31 5.0 8.3 T89-32 4.4

5.1 6.0 6.6 7.7

4.7 5.5 6.3 7.1

5.4 5.8 7.2 7.8

5.8 6.8 7.3 8.2

6.4 6.6 8.0 8.4 [00254] The results of the growth analysis are specified in Table 2.18 overall. The determined growth effects of the specified construction group are presented as ratios between

AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC of the construction group and the wild type group.

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Maximum growth rate in diameter CD CO o Maximum growth rate at maximum height 1.06 Maximum final diameter 1.02 Final maximum height 1.09 Average growth rate in diameter 0.91 Maximum growth rate at average height 1.04 Final average diameter 1.00 Final average height 1.06 Construction group TF0052Rp1

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Construction group TF0052Rp2 [00255] This construction induces increased growth. The final height is 13% higher when comparing the average of the construction group and the control group of the wild type. The final height is 13% higher when comparing the largest individuals in the construction group and the control group in the wild type. The growth rate at maximum height is 13% higher when comparing the average of the construction group and the control group of the wild type. The growth rate at maximum height is 18% higher when comparing the largest individuals in the construction group and the wild type control group. Construction group TF0076Rp2 meets the most stringent level of criterion (1) for selecting growth differences, as shown in Table 2.21.

[00256] Tables 2.19 and 2.20 contain growth data for the specified construction group and the corresponding wild type group. The columns in the tables contain the height and diameter measurements of individuals from the specified construction group and the corresponding wild type group. The measurement time, in the form of number of days in the greenhouse, is shown in the table headings. Table 2.19 Height growth data for TF0076Rp2

Height (cm)

Days in the greenhouse 20 25 29 35 41 46 50 53 57 60 TF0076rp2-3BB-1 15 22 29 43 59 72 84 93 103 111 TF0076rp2-3BB-2 20 28 39 53 70 85 95 104 115 120 TF0076rp2-3BB-3 17 25 33 48 67 81 92 101 112 122 TF0076rp2-4B-1 15 26 35 55 76 97 111 122 134 143 TF0076rp2-4B-2 19 28 37 53 72 89 103 111 124 135 TF0076rp2-4B-3 20 28 40 53 74 93 104 114 124 134 TF0076rp2-5BA-1 18 24 30 39 49 65 74 84 96 103 TF0076rp2-5BA-2 17 26 36 49 63 82 92 100 112 121 TF0076rp2-5BA-3 16 24 31 46 65 84 93 103 116 126 TF0076rp2-5BB-1 17 24 30 45 61 76 86 97 109 119 TF0076rp2-5BB-2 15 22 29 42 58 73 86 95 106 118 TF0076rp2-5BB-3 20 27 36 50 69 AT 94 100 109 118

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T89-01 17 26 31 45 61 76 87 95 107 116 T89-02 16 21 26 38 53 67 76 83 AT AT T89-03 18 24 32 47 64 78 87 96 106 115 T89-04 18 26 35 50 66 80 87 96 107 115 T89-05 17 26 33 44 57 73 81 91 100 107 T89-06 16 21 28 40 55 74 79 89 99 106 T89-07 17 23 31 43 57 71 80 88 98 107 T89-08 15 20 26 37 51 65 75 84 94 100 T89-09 18 25 32 46 61 74 85 93 103 112 T89-10 19 25 32 46 60 75 86 95 104 112 T89-11 20 27 36 51 68 86 98 107 120 127 T89-12 19 27 36 50 67 80 89 97 108 118 T89-13 18 25 32 45 59 75 83 92 101 108 T89-14 18 23 26 36 51 65 72 79 89 96 T89-15 17 21 28 39 53 70 78 87 97 105 T89-16 19 25 32 43 57 71 81 90 101 109 T89-17 16 20 29 41 54 68 78 84 93 101 T89-18 16 23 30 45 63 78 87 94 103 110 T89-19 16 22 28 42 56 69 79 89 100 107 T89-20 18 25 32 47 63 78 89 99 109 115 T89-21 19 27 34 50 67 82 92 102 111 120 T89-22 19 25 32 44 58 76 84 93 102 109 T89-23 18 26 33 47 63 79 88 99 108 116 T89-24 16 24 28 42 53 70 78 85 94 103 T89-25 16 25 32 45 61 76 85 95 107 114 T89-26 16 20 26 38 52 68 78 86 97 103 T89-27 16 21 25 35 48 61 70 78 AT 98 T89-28 14 18 25 38 51 64 73 81 90 96 T89-29 7 13 18 30 45 60 69 77 90 98 T89-30 15 22 29 42 55 69 80 88 97 106 T89-31 21 29 37 50 65 80 88 98 108 116 T89-32 19 24 32 42 55 70 78 87 96 105

Table 2.20 Diameter growth data for TF0076Rp2

Diânr etro mm) Days in the greenhouse 35 41 46 53 TF0076rp2-3BB-1 4.1 5.3 6.4 8.3 TF0076rp2-3BB-2 4.6 5.5 6.9 7.4 TF0076rp2-3BB-3 5.3 6.6 7.3 8.5

Diameter (mm)

60 Days in the greenhouse 35 41 46 53 60 8.2 T89-11 4.7 5.8 6.4 7.9 8.3 8.4 T89-12 4.7 5.9 7.3 7.4 7.6 9.1 T89-13 5.9 5.9 6.7 7.5 8.3

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TF0076rp2-4B-1 4.4 5.8 6.8 7.5 8.0 T89-14 4.5 5.2 5.8 6.1 7.0 TF0076rp2-4B-2 4.4 5.7 6.5 7.0 7.9 T89-15 4.7 AT 6.0 6.4 7.3 TF0076rp2-4B-3 4.9 6.2 7.5 8.2 9.1 T89-16 4.5 5.6 7.7 7.5 8.6 TF0076rp2-5BA-1 4.7 5.5 6.5 6.8 7.3 T89-17 4.1 5.4 6.0 6.8 7.5 TF0076rp2-5BA-2 4.4 6.2 6.4 7.4 8.1 T89-18 4.4 5.8 6.0 7.7 8.3 TF0076rp2-5BA-3 4.4 5.5 6.8 7.8 8.6 T89-19 4.7 5.8 7.1 8.5 9.4 TF0076rp2-5BB-1 4.5 5.8 6.1 7.3 8.0 T89-20 4.7 5.8 6.6 7.4 8.3 TF0076rp2-5BB-2 4.1 5.9 6.5 7.1 8.2 T89-21 4.7 6.2 6.3 7.7 8.5 TF0076rp2-5BB-3 4.1 6.1 6.1 6.4 8.1 T89-22 4.2 5.1 6.3 6.9 8.0 T89-01 4.7 6.0 6.5 6.9 8.3 T89-23 4.6 5.8 7.1 7.5 8.3 T89-02 4.4 5.7 6.6 7.6 7.8 T89-24 4.4 5.8 6.9 7.7 8.8 T89-03 4.7 6.3 6.7 7.7 8.4 T89-25 4.5 5.4 6.2 8.0 8.7 T89-04 4.7 6.0 7.1 8.0 8.5 T89-26 4.1 5.4 6.0 7.2 8.0 T89-05 4.0 5.3 6.3 7.8 8.1 T89-27 4.5 5.7 6.5 7.9 9.2 T89-06 4.6 5.9 6.3 7.7 8.2 T89-28 4.3 5.1 6.0 6.6 7.7 T89-07 4.6 6.1 6.5 7.9 8.5 T89-29 3.5 4.7 5.5 6.3 7.1 T89-08 4.0 5.2 5.8 6.9 7.1 T89-30 4.1 5.4 5.8 7.2 7.8 T89-09 4.4 5.8 6.4 7.6 8.2 T89-31 5.0 5.8 6.8 7.3 8.2 T89-10 4.9 6.0 6.6 7.4 8.5 T89-32 4.4 6.4 6.6 8.0 8.4

[00257] The results of the growth analysis are specified in general Table 2.21. The determined growth effects of the specified construction group are presented as ratios between AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC of the construction group and the wild type group.

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Maximum growth rate in diameter 1 ^ CO o Maximum growth rate at maximum height 00 Maximum final diameter 0.97 Final maximum height 1.13 Average growth rate in diameter 1.01 Maximum growth rate at average height 1.13 Final average diameter 1.01 Final average height 1.13 Construction group TF0076Rp2

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Construction group TF0096 [00258] This construction induces increased growth. The final height is 11% higher when comparing the average of the construction group and the control group of the wild type. The final height is 8% higher when comparing the largest individuals in the construction group and the wild type control group. The growth rate at maximum height is 18% higher when comparing the average of the construction group and the control group of the wild type. The growth rate at maximum height is 14% higher when comparing the largest individuals in the construction group and the wild type control group. The final diameter is 15% larger when compared to the average of the construction group and the control group of the wild type. The final diameter is 8% larger when comparing the larger individuals of the construction group and the control group of the wild type. The growth rate in diameter is 27% higher compared to the average of the construction group and the control group of the wild type. The growth rate in diameter is 14% higher when comparing the largest individuals in the construction group and the wild type control group. Construction group TF0096 meets the most stringent level of growth difference selection criteria (3) and the least stringent levels of growth difference selection criteria (1) and (2), as shown in Table 2.24.

[00259] Tables 2.22 and 2.23 contain growth data for the specified construction group and the corresponding wild type group. The columns in the tables contain the height and diameter measurements of individuals from the specified construction group and the corresponding wild type group. The measurement time, in the form of number of days in the greenhouse, is shown in the table headings.

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Table 2.22 Height growth data for TF0096

Height (cm)

Days in the greenhouse 18 25 28 32 36 39 46 53 55 TF0096-2A 14 27 41 51 68 80 109 138 148 TF0096-2B 13 24 36 47 62 72 101 126 131 TF0096-3A 12 26 38 50 65 77 106 133 141 TF0096-3B 16 29 39 50 62 72 101 127 134 TF0096-4A 24 42 54 67 82 93 120 AT 151 T89-01 18 32 43 54 66 76 98 124 133 T89-02 20 33 43 53 67 76 101 124 129 T89-03 17 31 43 53 68 76 102 128 135 T89-04 19 33 44 54 67 74 97 122 129 T89-05 20 33 44 54 67 76 100 121 129 T89-06 18 31 42 53 67 76 97 121 128 T89-07 16 28 41 53 65 74 96 121 129 T89-08 19 34 43 53 65 75 98 123 131 T89-09 20 30 40 52 64 73 97 119 125 T89-10 21 33 41 49 61 70 92 114 121 T89-11 18 31 40 49 61 71 94 117 123 T89-12 18 31 40 AT 60 68 90 107 115 T89-13 19 35 47 58 70 78 103 128 135 T89-14 19 31 40 50 61 72 93 118 124 T89-15 20 32 41 50 62 70 91 114 120 T89-16 18 30 39 49 62 71 92 114 122 T89-17 17 33 45 55 70 78 104 129 136 T89-18 19 32 44 56 71 82 106 130 137 T89-19 16 26 36 46 51 69 89 112 119 T89-20 16 31 41 49 60 71 91 111 118 T89-21 20 31 44 54 68 76 99 122 127 T89-22 14 26 37 46 61 72 96 120 127 T89-23 19 32 40 50 63 73 100 121 127 T89-24 20 31 41 47 58 68 90 111 117 T89-25 20 36 45 57 69 78 101 127 134

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T89-26 20 37 49 58 71 80 107 131 140 T89-27 19 34 44 55 71 81 107 131 138 T89-28 17 35 44 56 68 76 99 126 132 T89-29 17 32 45 55 68 78 101 125 132 T89-30 18 31 41 50 63 72 95 119 125 T89-31 17 27 35 45 58 67 87 108 116 T89-32 19 32 44 52 65 74 98 121 127 T89-33 20 33 43 53 64 74 96 119 126 T89-34 19 34 44 55 68 78 100 124 130 T89-35 17 30 40 51 64 74 95 116 122 T89-36 18 30 40 49 62 71 91 114 121 T89-82 21 33 43 54 65 75 98 118 125 T89-83 22 35 47 55 71 80 105 130 137 T89-85 21 36 46 56 67 76 98 123 131

Table 2.23 TF0096 diameter growth data

Days in the greenhouse 32 39 Diameter (mm) 46 53 55 TF0096-2A 4.8 6.0 7.3 8.4 8.6 TF0096-2B 4.4 6.2 8.2 9.2 9.9 TF0096-3A 5.5 6.8 7.4 9.2 9.2 TF0096-3B 3.9 5.5 7.3 8.2 8.4 TF0096-4A 5.5 6.9 7.6 9.2 9.1 T89-01 5.2 6.0 6.8 8.2 7.6 T89-02 4.5 5.8 7.1 8.6 8.3 T89-03 4.4 5.7 6.8 7.9 8.1 T89-04 4.4 6.0 6.9 8.6 8.5 T89-05 4.6 5.8 6.7 7.9 8.0 T89-06 4.7 5.9 7.2 8.1 9.2 T89-07 4.8 5.5 6.2 6.9 7.1 T89-08 4.5 5.4 6.0 6.9 7.1 T89-09 4.7 5.6 6.7 8.8 8.0 T89-10 4.1 4.9 5.6 7.1 7.0 T89-11 3.8 5.3 7.3 8.0 7.6

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T89-12 AT 5.1 6.2 7.3 6.9 T89-13 4.8 5.7 6.5 7.6 7.6 T89-14 4.2 5.5 6.6 7.6 6.5 T89-15 5.0 6.1 7.2 7.9 8.3 T89-16 4.4 5.6 6.2 7.3 7.9 T89-17 4.8 6.7 7.0 8.3 8.4 T89-18 4.2 5.5 6.7 7.3 7.4 T89-19 4.7 5.5 6.8 7.2 7.8 T89-20 4.5 5.6 6.5 7.0 7.3 T89-21 4.9 5.6 6.4 7.4 7.6 T89-22 4.3 5.4 6.5 7.3 7.6 T89-23 3.9 5.5 6.7 7.7 7.0 T89-24 4.3 5.9 6.8 8.1 7.9 T89-25 4.7 6.5 7.5 9.2 9.1 T89-26 5.4 5.9 7.7 8.6 8.8 T89-27 4.7 5.6 7.4 7.9 8.0 T89-28 4.7 5.7 6.3 7.2 7.4 T89-29 4.8 5.6 6.6 7.7 8.1 T89-30 4.6 5.5 6.7 7.3 7.3 T89-31 4.3 5.8 6.3 7.5 7.6 T89-32 4.6 5.9 7.6 9.3 9.1 T89-33 4.4 5.3 6.2 7.1 7.1 T89-34 4.6 5.6 6.8 8.1 8.7 T89-35 5.3 6.0 7.5 8.8 8.9 T89-36 4.4 6.1 6.9 8.1 8.6 T89-82 4.7 5.3 6.5 7.5 7.4 T89-83 4.7 6.1 7.1 8.3 8.3 T89-85 5.1 6.3 7.3 7.7 8.2

[00260] The results of the growth analysis are specified in Table 2.24 in general. The determined growth effects of the specified construction group are presented as ratios between AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC of the construction group and the wild type group.

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Maximum growth rate in diameter 1.14 Maximum growth rate at maximum height 1.14 Maximum final diameter 1.08 Final maximum height 1.08 Average growth rate in diameter 1.27 Maximum growth rate at average height CO Final average diameter 1.15 Final average height 1.11 Construction group TF0096

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Construction group TF0097Rp1 [00261] This construction induces increased growth. The final height is 33% higher when comparing the average of the construction group and the control group of the wild type. The final height is 43% higher when comparing the largest individuals in the construction group and the wild type control group. The growth rate at maximum height is 32% higher when comparing the average of the construction group and the control group of the wild type. The maximum height growth rate is 41% higher when comparing the largest individuals in the construction group and the wild type control group. The final diameter is 11% larger when compared to the average of the construction group and the control group of the wild type. The final diameter is 13% larger when comparing the larger individuals of the construction group and the control group of the wild type. The growth rate in diameter is 20% higher when comparing the average of the construction group and the control group of the wild type. The growth rate in diameter is 26% higher when comparing the largest individuals in the construction group and the wild type control group. Construction group TF0097Rp1 meets the most stringent levels of criteria (1), (2), (3) and (4) for selecting growth differences, as shown in Table 2.27.

[00262] Tables 2.25 and 2.26 contain growth data for the specified construction group and the corresponding wild type group. The columns in the tables contain the height and diameter measurements of individuals from the specified construction group and the corresponding wild type group. The measurement time, in the form of number of days in the greenhouse, is shown in the table headings.

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Table 2.25 Height growth data for TF0097Rp1

Height (cm)

Days in the greenhouse 20 25 29 35 41 46 50 53 57 60 TF0097rp1-1A-1 25 32 41 61 86 107 123 137 153 166 TF0097rp1-1A-2 29 40 52 69 91 113 129 140 155 170 TF0097rp1-1A-3 27 35 40 57 81 99 115 126 140 151 TF0097rp1-2A-1 23 32 43 61 75 92 102 111 123 132 TF0097rp1-2A-2 19 31 41 57 75 92 102 111 122 132 TF0097rp1-2A-3 19 29 38 52 68 86 94 103 112 121 TF0097rp1-2B-1 26 33 43 61 80 93 102 110 118 124 TF0097rp1-2B-2 26 34 45 62 83 102 114 125 138 147 TF0097rp1-2B-3 22 32 44 63 86 101 117 127 144 155 TF0097rp1-3A-1 27 40 49 72 96 115 137 150 165 181 TF0097rp1-3A-2 13 23 34 50 73 93 108 119 132 143 TF0097rp1-3A-3 19 31 44 63 88 109 125 137 151 162 TF0097rp1-4A-1 24 35 42 48 62 81 91 99 108 116 TF0097rp1-4A-2 14 22 28 39 55 80 90 103 115 126 TF0097rp1-4A-3 19 25 29 46 67 80 99 109 125 136 T89-01 17 26 31 45 61 76 87 95 107 116 T89-02 16 21 26 38 53 67 76 83 AT AT T89-03 18 24 32 47 64 78 87 96 106 115 T89-04 18 26 35 50 66 80 87 96 107 115 T89-05 17 26 33 44 57 73 81 91 100 107 T89-06 16 21 28 40 55 74 79 89 99 106 T89-07 17 23 31 43 57 71 80 88 98 107 T89-08 15 20 26 37 51 65 75 84 94 100 T89-09 18 25 32 46 61 74 85 93 103 112 T89-10 19 25 32 46 60 75 86 95 104 112 T89-11 20 27 36 51 68 86 98 107 120 127 T89-12 19 27 36 50 67 80 89 97 108 118 T89-13 18 25 32 45 59 75 83 92 101 108 T89-14 18 23 26 36 51 65 72 79 89 96 T89-15 17 21 28 39 53 70 78 87 97 105

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T89-16 19 25 32 43 57 71 81 90 101 109 T89-17 16 20 29 41 54 68 78 84 93 101 T89-18 16 23 30 45 63 78 87 94 103 110 T89-19 16 22 28 42 56 69 79 89 100 107 T89-20 18 25 32 47 63 78 89 99 109 115 T89-21 19 27 34 50 67 82 92 102 111 120 T89-22 19 25 32 44 58 76 84 93 102 109 T89-23 18 26 33 47 63 79 88 99 108 116 T89-24 16 24 28 42 53 70 78 85 94 103 T89-25 16 25 32 45 61 76 85 95 107 114 T89-26 16 20 26 38 52 68 78 86 97 103 T89-27 16 21 25 35 48 61 70 78 AT 98 T89-28 14 18 25 38 51 64 73 81 90 96 T89-29 7 13 18 30 45 60 69 77 90 98 T89-30 15 22 29 42 55 69 80 88 97 106 T89-31 21 29 37 50 65 80 88 98 108 116 T89-32 19 24 32 42 55 70 78 87 96 105

Table 2.26 Diameter growth data for TF0097Rp1

Days in the greenhouse 35 41 Diameter (mm) 46 53 60 TF0097rp1-1A-1 4.3 5.3 6.2 8.0 9.5 TF0097rp1-1A-2 4.5 5.3 6.0 7.4 8.8 TF0097rp1-1A-3 4.4 5.2 5.3 6.4 8.1 TF0097rp1-2A-1 5.7 6.9 7.8 9.6 9.9 TF0097rp1-2A-2 5.3 6.7 7.5 7.9 9.3 TF0097rp1-2A-3 5.1 6.5 7.5 7.8 9.2 TF0097rp1-2B-1 4.6 5.5 6.2 6.8 7.5 TF0097rp1-2B-2 4.9 6.0 7.1 7.6 10.2 TF0097rp1-2B-3 5.3 7.7 8.2 9.9 10.6 TF0097rp1-3A-1 5.7 7.0 7.7 9.4 9.8 TF0097rp1-3A-2 4.7 6.2 7.0 7.3 9.5

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TF0097rp1-3A-3 5.0 6.5 7.1 9.0 10.2 TF0097rp1-4A-1 4.4 5.0 6.0 7.3 6.7 TF0097rp1-4A-2 4.1 5.4 5.9 6.3 7.2 TF0097rp1-4A-3 3.7 4.9 6.0 8.6 9.4 T89-01 4.7 6.0 6.5 6.9 8.3 T89-02 4.4 5.7 6.6 7.6 7.8 T89-03 4.7 6.3 6.7 7.7 8.4 T89-04 4.7 6.0 7.1 8.0 8.5 T89-05 4.0 5.3 6.3 7.8 8.1 T89-06 4.6 5.9 6.3 7.7 8.2 T89-07 4.6 6.1 6.5 7.9 8.5 T89-08 4.0 5.2 5.8 6.9 7.1 T89-09 4.4 5.8 6.4 7.6 8.2 T89-10 4.9 6.0 6.6 7.4 8.5 T89-11 4.7 5.8 6.4 7.9 8.3 T89-12 4.7 5.9 7.3 7.4 7.6 T89-13 5.9 5.9 6.7 7.5 8.3 T89-14 4.5 5.2 5.8 6.1 7.0 T89-15 4.7 AT 6.0 6.4 7.3 T89-16 4.5 5.6 7.7 7.5 8.6 T89-17 4.1 5.4 6.0 6.8 7.5 T89-18 4.4 5.8 6.0 7.7 8.3 T89-19 4.7 5.8 7.1 8.5 9.4 T89-20 4.7 5.8 6.6 7.4 8.3 T89-21 4.7 6.2 6.3 7.7 8.5 T89-22 4.2 5.1 6.3 6.9 8.0 T89-23 4.6 5.8 7.1 7.5 8.3 T89-24 4.4 5.8 6.9 7.7 8.8 T89-25 4.5 5.4 6.2 8.0 8.7 T89-26 4.1 5.4 6.0 7.2 8.0

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T89-27 4.5 5.7 6.5 7.9 9.2 T89-28 4.3 5.1 6.0 6.6 7.7 T89-29 3.5 4.7 5.5 6.3 7.1 T89-30 4.1 5.4 5.8 7.2 7.8 T89-31 5.0 5.8 6.8 7.3 8.2 T89-32 4.4 6.4 6.6 8.0 8.4

[00263] The results of the growth analysis are specified in Table 2.27 overall. The determined growth effects of the specified construction group are presented as ratios between AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC of the construction group and the wild type group.

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Maximum growth rate in diameter 1.26 Maximum growth rate at maximum height 1.41 Maximum final diameter 1.13 Final maximum height 1.43 Average growth rate in diameter 1.20 Maximum growth rate at average height 1.32 Final average diameter 1.11 Final average height 1.33 Construction group TF0097Rp1

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Construction group TF0104 [00264] This construction induces increased growth. The final height is 15% higher when comparing the average of the construction group and the control group of the wild type. The final height is 12% higher when comparing the largest individuals in the construction group and the control group in the wild type. The growth rate at maximum height is 16% higher when comparing the average of the construction group and the control group of the wild type. The growth rate at maximum height is 14% higher when comparing the largest individuals in the construction group and the wild type control group. The final diameter is 23% bigger when comparing the average of the construction group and the control group of the wild type. The final diameter is 20% larger when comparing the larger individuals of the construction group and the control group of the wild type. The growth rate in diameter is 20% higher when comparing the average of the construction group and the control group of the wild type. The growth rate in diameter is 10% higher when comparing the largest individuals in the construction group and the wild type control group. Construction group TF0104 meets the most stringent levels of growth differences selection criteria (1), (2) and (3) and the least difference level of growth differences selection criterion (4), as shown in the table 2.30.

[00265] Tables 2.28 and 2.29 contain growth data for the specified construction group and the corresponding wild type group. The columns in the tables contain the height and diameter measurements of individuals from the specified construction group and the corresponding wild type group. The measurement time, in the form of number of days in the greenhouse, is shown in the table headings.

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Table 2.28 Height growth data for TF0104

Height (cm)

Days in the greenhouse 18 25 28 32 36 39 46 53 55 TF0104-1A 20 35 48 60 77 90 117 149 157 TF0104-1B 23 37 50 63 78 90 120 148 155 TF0104-2A 21 37 50 62 75 85 114 140 147 TF0104-3A 20 37 50 61 74 85 102 115 119 TF0104-3B 20 35 50 62 78 89 118 145 152 T89-01 18 32 43 54 66 76 98 124 133 T89-02 20 33 43 53 67 76 101 124 129 T89-03 17 31 43 53 68 76 102 128 135 T89-04 19 33 44 54 67 74 97 122 129 T89-05 20 33 44 54 67 76 100 121 129 T89-06 18 31 42 53 67 76 97 121 128 T89-07 16 28 41 53 65 74 96 121 129 T89-08 19 34 43 53 65 75 98 123 131 T89-09 20 30 40 52 64 73 97 119 125 T89-10 21 33 41 49 61 70 92 114 121 T89-11 18 31 40 49 61 71 94 117 123 T89-12 18 31 40 AT 60 68 90 107 115 T89-13 19 35 47 58 70 78 103 128 135 T89-14 19 31 40 50 61 72 93 118 124 T89-15 20 32 41 50 62 70 91 114 120 T89-16 18 30 39 49 62 71 92 114 122 T89-17 17 33 45 55 70 78 104 129 136 T89-18 19 32 44 56 71 82 106 130 137 T89-19 16 26 36 46 51 69 89 112 119 T89-20 16 31 41 49 60 71 91 111 118 T89-21 20 31 44 54 68 76 99 122 127 T89-22 14 26 37 46 61 72 96 120 127 T89-23 19 32 40 50 63 73 100 121 127 T89-24 20 31 41 47 58 68 90 111 117 T89-25 20 36 45 57 69 78 101 127 134

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T89-26 20 37 49 58 71 80 107 131 140 T89-27 19 34 44 55 71 81 107 131 138 T89-28 17 35 44 56 68 76 99 126 132 T89-29 17 32 45 55 68 78 101 125 132 T89-30 18 31 41 50 63 72 95 119 125 T89-31 17 27 35 45 58 67 87 108 116 T89-32 19 32 44 52 65 74 98 121 127 T89-33 20 33 43 53 64 74 96 119 126 T89-34 19 34 44 55 68 78 100 124 130 T89-35 17 30 40 51 64 74 95 116 122 T89-36 18 30 40 49 62 71 91 114 121 T89-82 21 33 43 54 65 75 98 118 125 T89-83 22 35 47 55 71 80 105 130 137 T89-85 21 36 46 56 67 76 98 123 131

Table 2.29 TF104 diameter growth data

Greenhouse days 32 39 Diameter (mm) 46 53 55 TF0104-1A 6.0 7.2 8.7 10.4 11.0 TF0104-1B 5.7 7.9 8.8 10.0 10.6 TF0104-2A 5.7 8.0 9.0 10.4 10.2 TF0104-3A 5.3 6.3 6.7 7.5 7.1 TF0104-3B 5.7 6.9 8.2 9.8 9.5 T89-01 5.2 6.0 6.8 8.2 7.6 T89-02 4.5 5.8 7.1 8.6 8.3 T89-03 4.4 5.7 6.8 7.9 8.1 T89-04 4.4 6.0 6.9 8.6 8.5 T89-05 4.6 5.8 6.7 7.9 8.0 T89-06 4.7 5.9 7.2 8.1 9.2 T89-07 4.8 5.5 6.2 6.9 7.1 T89-08 4.5 5.4 6.0 6.9 7.1 T89-09 4.7 5.6 6.7 8.8 8.0 T89-10 4.1 4.9 5.6 7.1 7.0 T89-11 3.8 5.3 7.3 8.0 7.6

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T89-12 AT 5.1 6.2 7.3 6.9 T89-13 4.8 5.7 6.5 7.6 7.6 T89-14 4.2 5.5 6.6 7.6 6.5 T89-15 5.0 6.1 7.2 7.9 8.3 T89-16 4.4 5.6 6.2 7.3 7.9 T89-17 4.8 6.7 7.0 8.3 8.4 T89-18 4.2 5.5 6.7 7.3 7.4 T89-19 4.7 5.5 6.8 7.2 7.8 T89-20 4.5 5.6 6.5 7.0 7.3 T89-21 4.9 5.6 6.4 7.4 7.6 T89-22 4.3 5.4 6.5 7.3 7.6 T89-23 3.9 5.5 6.7 7.7 7.0 T89-24 4.3 5.9 6.8 8.1 7.9 T89-25 4.7 6.5 7.5 9.2 9.1 T89-26 5.4 5.9 7.7 8.6 8.8 T89-27 4.7 5.6 7.4 7.9 8.0 T89-28 4.7 5.7 6.3 7.2 7.4 T89-29 4.8 5.6 6.6 7.7 8.1 T89-30 4.6 5.5 6.7 7.3 7.3 T89-31 4.3 5.8 6.3 7.5 7.6 T89-32 4.6 5.9 7.6 9.3 9.1 T89-33 4.4 5.3 6.2 7.1 7.1 T89-34 4.6 5.6 6.8 8.1 8.7 T89-35 5.3 6.0 7.5 8.8 8.9 T89-36 4.4 6.1 6.9 8.1 8.6 T89-82 4.7 5.3 6.5 7.5 7.4 T89-83 4.7 6.1 7.1 8.3 8.3 T89-85 5.1 6.3 7.3 7.7 8.2

[00266] The results of the growth analysis are specified in general Table 2.30. The determined growth effects of the specified construction group are presented as ratios between AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC of the construction group and the wild type group.

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Maximum growth rate in diameter 1.10 Maximum growth rate at maximum height 1.14 Maximum final diameter 1.20 Final maximum height 1.12 Average growth rate in diameter 1.20 Maximum growth rate at average height 1.16 Final average diameter 1.23 Final average height 1.15 Construction group the o LL H

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Construction group TF0109Rp1 [00267] This construction induces increased growth. The final height is 22% higher when comparing the average of the construction group and the control group of the wild type. The final height is 32% higher when comparing the largest individuals in the construction group and the control group in the wild type. The growth rate at maximum height is 26% higher when comparing the average of the construction group and the control group of the wild type. The maximum height growth rate is 40% higher when comparing the largest individuals in the construction group and the wild type control group. The final diameter is 15% larger when compared to the average of the construction group and the control group of the wild type. The final diameter is 14% larger when comparing the larger individuals of the construction group and the control group of the wild type. The growth rate in diameter is 25% higher compared to the average of the construction group and the control group of the wild type. The growth rate in diameter is 27% higher when comparing the largest individuals in the construction group and the wild type control group. Construction group TF0109Rp1 meets the most stringent levels of criteria (1), (2), (3) and (4) for selection of growth differences, as shown in table 2.30.

[00268] Tables 2.31 and 2.32 contain growth data for the specified construction group and the corresponding wild type group. The columns in the tables contain the height and diameter measurements of individuals from the specified construction group and the corresponding wild type group. The measurement time, in the form of number of days in the greenhouse, is shown in the table headings.

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Table 2.31 Height growth data for TF0109Rp1

Height (cm)

Days in the greenhouse 20 25 29 35 41 46 50 53 57 60 TF0109rp1-2A-1 21 31 39 55 73 89 100 109 122 130 TF0109rp1-2A-2 18 25 33 47 65 79 91 101 111 120 TF0109rp1-2A-3 17 23 31 47 63 77 87 96 109 118 TF0109rp1-2B-1 19 26 35 54 78 103 117 129 141 149 TF0109rp1-2B-2 21 31 39 57 82 105 124 136 151 168 TF0109rp1-2B-3 16 27 40 51 80 102 117 122 137 150 TF0109rp1-3B-1 18 29 39 59 77 94 104 114 126 136 TF0109rp1-3B-2 18 28 37 54 70 88 98 108 118 125 TF0109rp1-3B-3 17 28 37 51 65 87 94 103 115 120 TF0109rp1-4A-1 16 25 32 46 64 79 94 104 116 126 TF0109rp1-4A-2 17 24 32 46 65 83 94 106 118 126 TF0109rp1-4A-3 17 19 26 38 57 73 85 96 110 121 T89-01 17 26 31 45 61 76 87 95 107 116 T89-02 16 21 26 38 53 67 76 83 AT AT T89-03 18 24 32 47 64 78 87 96 106 115 T89-04 18 26 35 50 66 80 87 96 107 115 T89-05 17 26 33 44 57 73 81 91 100 107 T89-06 16 21 28 40 55 74 79 89 99 106 T89-07 17 23 31 43 57 71 80 88 98 107 T89-08 15 20 26 37 51 65 75 84 94 100 T89-09 18 25 32 46 61 74 85 93 103 112 T89-10 19 25 32 46 60 75 86 95 104 112 T89-11 20 27 36 51 68 86 98 107 120 127 T89-12 19 27 36 50 67 80 89 97 108 118 T89-13 18 25 32 45 59 75 83 92 101 108 T89-14 18 23 26 36 51 65 72 79 89 96 T89-15 17 21 28 39 53 70 78 87 97 105 T89-16 19 25 32 43 57 71 81 90 101 109 T89-17 16 20 29 41 54 68 78 84 93 101 T89-18 16 23 30 45 63 78 87 94 103 110

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T89-19 16 22 28 42 56 69 79 89 100 107 T89-20 18 25 32 47 63 78 89 99 109 115 T89-21 19 27 34 50 67 82 92 102 111 120 T89-22 19 25 32 44 58 76 84 93 102 109 T89-23 18 26 33 47 63 79 88 99 108 116 T89-24 16 24 28 42 53 70 78 85 94 103 T89-25 16 25 32 45 61 76 85 95 107 114 T89-26 16 20 26 38 52 68 78 86 97 103 T89-27 16 21 25 35 48 61 70 78 AT 98 T89-28 14 18 25 38 51 64 73 81 90 96 T89-29 7 13 18 30 45 60 69 77 90 98 T89-30 15 22 29 42 55 69 80 88 97 106 T89-31 21 29 37 50 65 80 88 98 108 116 T89-32 19 24 32 42 55 70 78 87 96 105

Table 2.32 Diameter growth data for TF109Rp1

Days in the greenhouse 35 41 Diameter (mm) 46 53 60 TF0109rp1-2A-1 5.7 7.3 8.1 9.1 9.9 TF0109rp1-2A-2 4.7 5.7 6.7 7.8 8.8 TF0109rp1-2A-3 4.6 6.2 6.5 8.1 9.3 TF0109rp1-2B-1 3.8 5.2 5.6 6.7 8.0 TF0109rp1-2B-2 4.3 5.4 6.3 6.9 7.9 TF0109rp1-2B-3 5.1 6.2 7.3 8.2 9.3 TF0109rp1-3B-1 5.1 7.0 7.3 7.7 9.1 TF0109rp1-3B-2 5.3 6.1 7.5 7.7 8.7 TF0109rp1-3B-3 4.9 5.3 6.7 8.1 9.3 TF0109rp1-4A-1 5.2 6.5 7.5 8.7 10.5 TF0109rp1-4A-2 5.4 6.5 7.8 9.2 10.7 TF0109rp1-4A-3 4.8 6.2 7.3 9.1 10.7 T89-01 4.7 6.0 6.5 6.9 8.3 T89-02 4.4 5.7 6.6 7.6 7.8 T89-03 4.7 6.3 6.7 7.7 8.4 T89-04 4.7 6.0 7.1 8.0 8.5

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T89-05 4.0 5.3 6.3 7.8 8.1 T89-06 4.6 5.9 6.3 7.7 8.2 T89-07 4.6 6.1 6.5 7.9 8.5 T89-08 4.0 5.2 5.8 6.9 7.1 T89-09 4.4 5.8 6.4 7.6 8.2 T89-10 4.9 6.0 6.6 7.4 8.5 T89-11 4.7 5.8 6.4 7.9 8.3 T89-12 4.7 5.9 7.3 7.4 7.6 T89-13 5.9 5.9 6.7 7.5 8.3 T89-14 4.5 5.2 5.8 6.1 7.0 T89-15 4.7 AT 6.0 6.4 7.3 T89-16 4.5 5.6 7.7 7.5 8.6 T89-17 4.1 5.4 6.0 6.8 7.5 T89-18 4.4 5.8 6.0 7.7 8.3 T89-19 4.7 5.8 7.1 8.5 9.4 T89-20 4.7 5.8 6.6 7.4 8.3 T89-21 4.7 6.2 6.3 7.7 8.5 T89-22 4.2 5.1 6.3 6.9 8.0 T89-23 4.6 5.8 7.1 7.5 8.3 T89-24 4.4 5.8 6.9 7.7 8.8 T89-25 4.5 5.4 6.2 8.0 8.7 T89-26 4.1 5.4 6.0 7.2 8.0 T89-27 4.5 5.7 6.5 7.9 9.2 T89-28 4.3 5.1 6.0 6.6 7.7 T89-29 3.5 4.7 5.5 6.3 7.1 T89-30 4.1 5.4 5.8 7.2 7.8 T89-31 5.0 5.8 6.8 7.3 8.2 T89-32 4.4 6.4 6.6 8.0 8.4

[00269] The results of the growth analysis are specified in general Table 2.33. The determined growth effects of the specified construction group are presented as ratios between AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC of the construction group and the wild type group.

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Maximum growth rate in diameter 1.27 Maximum growth rate at maximum height 1.40 Maximum final diameter 1.14 Final maximum height 1.32 Average growth rate in diameter 1.25 Maximum growth rate at average height 1.26 Final average diameter 1.15 Final average height 1.22 Construction group TF0109Rp1

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Construction group TF0116 [00270] Tables 2.34 and 2.35 contain growth data for the specified construction group and the corresponding wild type group. The columns in the tables contain the height and diameter measurements of individuals from the specified construction group and the corresponding wild type group. The measurement time, in the form of number of days in the greenhouse, is shown in the table headings. Table 2.34 Height growth data for TF0116

Days in the greenhouse 19 28 34 40 Height (cm) 54 61 65 44 47 51 TF0116-1B AT AT AT AT AT AT AT AT AT AT TF0116-2A 18 37 51 67 77 86 98 106 123 133 TF0116-2B-1 18 34 50 65 76 84 98 106 126 139 TF0116-2B-2 17 31 48 61 75 82 93 101 120 133 TF0116-4A 16 33 53 68 81 91 103 113 133 142 TF0116-5B 21 40 56 70 78 88 98 105 124 136 TF0116-6A 21 36 55 71 83 90 101 110 131 143 TF0116-6B 17 29 45 59 70 80 92 102 125 138 T89-01 18 30 46 58 69 77 87 96 113 122 T89-02 18 30 49 62 72 77 84 90 102 109 T89-03 15 27 41 54 65 73 82 91 112 123 T89-10 AT AT AT AT AT AT AT AT AT AT T89-11 19 31 49 61 74 83 94 103 124 133 T89-12 17 30 45 58 69 77 90 100 123 134 T89-13 18 27 43 56 68 78 91 100 121 133 T89-14 5 26 42 56 67 74 83 90 109 119 T89-15 10 15 25 33 41 45 52 57 72 82 T89-16 19 33 53 63 73 82 93 102 119 130 T89-17 17 29 46 58 66 72 80 86 96 102 T89-18 16 30 48 59 71 81 93 103 122 132 T89-19 18 32 50 65 77 84 94 102 126 139 T89-20 16 33 52 67 79 88 98 105 124 139

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T89-21 17 29 44 58 67 73 83 90 110 122 T89-22 14 28 47 61 71 80 89 98 119 133 T89-23 10 33 45 53 63 71 82 90 112 123 T89-24 20 28 43 51 62 69 78 87 106 119 T89-25 14 26 38 52 64 72 82 89 110 122 T89-26 15 28 44 57 69 77 87 96 120 133 T89-27 18 29 47 62 75 82 92 103 125 138 T89-28 22 37 54 67 78 88 97 104 123 133 T89-29 16 33 49 63 76 84 93 100 123 138 T89-30 15 40 46 58 67 74 85 92 113 124

Table 2.35 - Diameter growth data for TF0116

Greenhouse days 28 34 40 Diameter (mm) 44 47 51 54 61 65 TF0116-1B AT AT AT AT AT AT AT AT AT TF0116-2A 3.7 5.9 7.3 8.2 8.6 9.2 10.0 10.5 10.9 TF0116-2B-1 3.5 5.3 6.2 6.9 8.0 8.4 9.0 10.0 10.2 TF0116-2B-2 3.4 5.0 5.8 6.6 7.3 8.4 8.4 10.7 10.3 TF0116-4A 3.8 5.8 6.5 7.2 8.0 8.7 9.4 10.2 10.9 TF0116-5B 3.9 5.0 6.3 6.9 7.6 8.2 8.4 10.3 10.4 TF0116-6A 3.9 4.9 6.7 7.5 7.9 9.0 9.4 10.7 11.1 TF0116-6B 3.7 4.7 5.9 7.2 7.7 9.2 9.7 10.5 10.8 T89-01 3.2 4.6 5.4 6.2 6.8 7.9 8.3 9.5 9.3 T89-02 3.4 4.7 5.5 7.3 6.3 6.6 6.9 8.3 7.5 T89-03 3.9 4.4 5.2 6.2 6.4 7.8 7.6 9.7 9.4 T89-10 AT AT AT AT AT AT AT AT AT T89-11 3.4 5.1 6.2 7.6 6.8 6.9 7.7 10.1 9.5 T89-12 2.9 4.7 5.8 6.6 7.8 8.0 8.7 9.1 9.2 T89-13 3.0 4.3 5.4 5.8 6.4 7.6 7.8 8.2 8.8 T89-14 3.0 4.5 5.7 6.3 6.7 7.9 7.8 9.0 8.9 T89-15 AT 2.1 3.0 4.0 4.0 4.5 4.9 5.5 5.6 T89-16 3.5 5.3 6.4 6.6 7.0 7.2 8.3 8.2 9.0 T89-17 3.4 4.6 5.1 5.4 6.0 6.4 6.5 6.8 7.1 T89-18 3.6 5.2 6.0 7.0 7.8 8.2 9.9 10.3 9.7

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T89-19 4.2 5.5 6.6 7.7 8.5 8.9 9.5 11.1 12.3 T89-20 4.1 5.5 6.6 8.1 9.3 9.6 9.3 10.0 11.1 T89-21 3.1 5.6 5.8 6.7 7.1 7.8 8.4 9.7 10.1 T89-22 3.2 4.4 5.6 6.5 7.5 7.6 7.8 8.9 9.2 T89-23 2.4 4.2 5.1 6.1 6.5 7.5 10.1 9.3 10.2 T89-24 3.2 4.5 5.1 6.3 7.0 7.6 8.1 8.8 9.1 T89-25 3.3 4.3 5.2 5.8 6.5 7.4 7.8 9.3 9.7 T89-26 3.3 4.4 5.5 6.6 7.2 8.1 8.9 9.5 10.5 T89-27 3.3 4.9 6.0 7.8 8.0 8.9 9.7 11.2 11.5 T89-28 4.5 5.7 7.4 7.8 8.5 9.4 9.7 10.2 11.1 T89-29 3.1 4.7 6.3 7.2 7.9 9.2 9.8 11.1 10.7 T89-30 3.0 5.8 6.2 7.7 7.9 8.6 8.2 10.1 10.3

[00271] The results of the growth analysis are specified in general Table 2.36. The determined growth effects of the specified construction group are presented as ratios between AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC of the construction group and the wild type group.

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Maximum growth rate in diameter 0.90 Maximum growth rate at maximum height 0.99 Maximum final diameter 0.90 Final maximum height 1.03 Average growth rate in diameter 1.13 Maximum growth rate at average height 1.03 Final average diameter 1.12 Final average height 1.10 Construction group TF0116

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Construction group TF0132.2nd [00272] This construction induces increased growth. The final height is 27% higher when comparing the average of the construction group and the control group of the wild type. The final height is 32% higher when comparing the largest individuals in the construction group and the control group in the wild type. The growth rate at maximum height is 38% higher when comparing the average of the construction group and the control group of the wild type. The maximum height growth rate is 41% higher when comparing the largest individuals in the construction group and the wild type control group. The final diameter is 12% larger when comparing the average of the construction group and the control group of the wild type. The final diameter is 9% larger when comparing the larger individuals of the construction group and the control group of the wild type. The growth rate in diameter is 15% higher compared to the average of the construction group and the control group of the wild type. The growth rate in diameter is 8% higher when comparing the largest individuals in the construction group and the wild type control group. Construction group TF0132.2nd meets the most stringent level of growth differences selection criteria (1), (3) and (4) and the least difference level of growth differences selection criterion (2), as shown in Table 2.39.

[00273] Tables 2.37 and 2.38 contain growth data for the specified construction group and the corresponding wild type group. The columns in the tables contain the height and diameter measurements of individuals from the specified construction group and the corresponding wild type group. The measurement time, in the form of number of days in the greenhouse, is shown in the table headings.

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Table 2.37 Height growth data for TF0132.2nd

Height (cm)

Days in the greenhouse 18 25 28 32 36 39 46 53 55 TF0132.2nd-1A 17 30 40 51 66 78 103 132 139 TF0132.2nd-1B 19 34 48 63 82 96 132 168 178 TF0132.2nd-2A 19 32 40 52 67 77 93 108 112 TF0132.2nd-4B 22 41 57 73 93 106 138 175 185 TF0132.2nd-5A 19 33 47 62 73 84 110 143 153 TF0132.2nd-5B 19 35 49 63 84 101 136 168 177 TF0132.2nd-6B 21 37 51 64 83 98 133 170 180 TF0132.2nd-7A 20 34 47 59 77 92 125 160 175 T89-01 18 32 43 54 66 76 98 124 133 T89-02 20 33 43 53 67 76 101 124 129 T89-03 17 31 43 53 68 76 102 128 135 T89-04 19 33 44 54 67 74 97 122 129 T89-05 20 33 44 54 67 76 100 121 129 T89-06 18 31 42 53 67 76 97 121 128 T89-07 16 28 41 53 65 74 96 121 129 T89-08 19 34 43 53 65 75 98 123 131 T89-09 20 30 40 52 64 73 97 119 125 T89-10 21 33 41 49 61 70 92 114 121 T89-11 18 31 40 49 61 71 94 117 123 T89-12 18 31 40 AT 60 68 90 107 115 T89-13 19 35 47 58 70 78 103 128 135 T89-14 19 31 40 50 61 72 93 118 124 T89-15 20 32 41 50 62 70 91 114 120 T89-16 18 30 39 49 62 71 92 114 122 T89-17 17 33 45 55 70 78 104 129 136 T89-18 19 32 44 56 71 82 106 130 137 T89-19 16 26 36 46 51 69 89 112 119 T89-20 16 31 41 49 60 71 91 111 118 T89-21 20 31 44 54 68 76 99 122 127 T89-22 14 26 37 46 61 72 96 120 127

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T89-23 19 32 40 50 63 73 100 121 127 T89-24 20 31 41 47 58 68 90 111 117 T89-25 20 36 45 57 69 78 101 127 134 T89-26 20 37 49 58 71 80 107 131 140 T89-27 19 34 44 55 71 81 107 131 138 T89-28 17 35 44 56 68 76 99 126 132 T89-29 17 32 45 55 68 78 101 125 132 T89-30 18 31 41 50 63 72 95 119 125 T89-31 17 27 35 45 58 67 87 108 116 T89-32 19 32 44 52 65 74 98 121 127 T89-33 20 33 43 53 64 74 96 119 126 T89-34 19 34 44 55 68 78 100 124 130 T89-35 17 30 40 51 64 74 95 116 122 T89-36 18 30 40 49 62 71 91 114 121 T89-82 21 33 43 54 65 75 98 118 125 T89-83 22 35 47 55 71 80 105 130 137 T89-85 21 36 46 56 67 76 98 123 131

Table 2.38 Data for diameter growth for TF0132.2nd

Days in the greenhouse 32 39 Diameter (mm) 46 53 55 TF0132.2nd-1A 4.7 6.1 7.5 8.8 9.3 TF0132.2nd-1B 5.3 6.3 7.6 10.2 10.0 TF0132.2nd-2A 5.0 6.0 6.5 7.2 6.7 TF0132.2nd-4B 5.8 6.5 7.5 9.6 9.6 TF0132.2nd-5A 5.1 6.0 6.4 7.7 8.0 TF0132.2nd-5B 5.2 6.4 7.2 8.4 8.3 TF0132.2nd-6B 4.9 6.3 7.6 9.1 9.3 TF0132.2nd-7A 4.9 6.7 8.0 9.1 9.5 T89-01 5.2 6.0 6.8 8.2 7.6 T89-02 4.5 5.8 7.1 8.6 8.3 T89-03 4.4 5.7 6.8 7.9 8.1 T89-04 4.4 6.0 6.9 8.6 8.5 T89-05 4.6 5.8 6.7 7.9 8.0

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T89-06 4.7 5.9 7.2 8.1 9.2 T89-07 4.8 5.5 6.2 6.9 7.1 T89-08 4.5 5.4 6.0 6.9 7.1 T89-09 4.7 5.6 6.7 8.8 8.0 T89-10 4.1 4.9 5.6 7.1 7.0 T89-11 3.8 5.3 7.3 8.0 7.6 T89-12 AT 5.1 6.2 7.3 6.9 T89-13 4.8 5.7 6.5 7.6 7.6 T89-14 4.2 5.5 6.6 7.6 6.5 T89-15 5.0 6.1 7.2 7.9 8.3 T89-16 4.4 5.6 6.2 7.3 7.9 T89-17 4.8 6.7 7.0 8.3 8.4 T89-18 4.2 5.5 6.7 7.3 7.4 T89-19 4.7 5.5 6.8 7.2 7.8 T89-20 4.5 5.6 6.5 7.0 7.3 T89-21 4.9 5.6 6.4 7.4 7.6 T89-22 4.3 5.4 6.5 7.3 7.6 T89-23 3.9 5.5 6.7 7.7 7.0 T89-24 4.3 5.9 6.8 8.1 7.9 T89-25 4.7 6.5 7.5 9.2 9.1 T89-26 5.4 5.9 7.7 8.6 8.8 T89-27 4.7 5.6 7.4 7.9 8.0 T89-28 4.7 5.7 6.3 7.2 7.4 T89-29 4.8 5.6 6.6 7.7 8.1 T89-30 4.6 5.5 6.7 7.3 7.3 T89-31 4.3 5.8 6.3 7.5 7.6 T89-32 4.6 5.9 7.6 9.3 9.1 T89-33 4.4 5.3 6.2 7.1 7.1 T89-34 4.6 5.6 6.8 8.1 8.7 T89-35 5.3 6.0 7.5 8.8 8.9 T89-36 4.4 6.1 6.9 8.1 8.6 T89-82 4.7 5.3 6.5 7.5 7.4 T89-83 4.7 6.1 7.1 8.3 8.3

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Τ89-85 | 5.1 6.3 7.3 7.7 8.2 [00274] The results of the growth analysis are specified in general Table 2.39. The determined growth effects of the specified construction group are presented as ratios between AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC of the construction group and the wild type group.

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Maximum growth rate in diameter 1.08 Maximum growth rate at maximum height 1.41 Maximum final diameter 1.09 Final maximum height 1.32 Average growth rate in diameter 1.15 Maximum growth rate at average height 1.38 Final average diameter 1.12 Final average height 1.27 Construction group TF0132.2n d

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Construction Group TF0132rp1 [00275] This construction induces increased growth. The final height is 29% higher compared to the average of the construction group and the control group of the wild type. The final height is 28% higher compared to the larger individuals in the construction group and the wild control group. The maximum height growth rate is 31% higher compared to the average for the construction group and the wild-type control group. The maximum height growth rate is 23% higher than that of the largest individuals in the construction group and in the wild control group.

[00276] Construction group TF0132p1 satisfies the most severe level of the selection criteria for growth differences (1), (3) and (4) as shown in table 2.42.

[00277] Tables 2.40 and 2.41 contain growth data for the specified construction group and the corresponding wild type group. The columns in the Tables contain height and diameter measurements of the individuals in the specified construction group and the corresponding wild type group. The measurement time as a number of days in the greenhouse is shown in the table headings.

Table 2.40 Growth height data for TF0132rp1

Height (cm)

Days in the greenhouse 15 19 26 33 37 40 47 51 54 TF0132rp1-1B-1 25 30 48 70 88 103 137 153 170 TF0132rp1-1B-2 23 26 38 63 80 96 133 151 168 TF0132rp1-1B-3 23 27 42 66 86 98 131 151 167 TF0132rp1-3BB-1 21 25 36 58 76 90 124 140 157 TF0132rp1-3BB-2 21 28 46 68 88 101 135 147 171 TF0132rp1-3BB-3 18 22 38 66 81 94 129 144 161 TF0132rp1-4AC-1 22 27 42 65 83 95 127 142 159 TF0132rp1-4AC-2 20 25 41 65 81 94 125 140 156 TF0132rp1-4AC-3 21 24 38 61 77 88 122 139 154

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TF0132rp1-4B-1 26 30 45 70 87 100 133 144 162 TF0132rp1-4B-2 20 26 43 64 77 90 121 135 152 TF0132rp1-4B-3 20 24 41 67 85 100 132 147 163 TF0132rp1-6B-1 23 26 41 65 83 95 127 141 157 TF0132rp1-6B-2 23 28 41 64 82 96 132 152 165 TF0132rp1-6B-3 20 26 45 73 93 107 140 156 169 T89-20 21 26 39 58 73 82 111 130 134 T89-21 21 24 34 51 64 72 97 103 125 T89-22 19 23 32 48 60 70 96 111 123 T89-23 21 24 34 51 63 70 94 112 122 T89-24 19 22 30 46 59 68 90 103 115 T89-25 22 30 39 57 72 81 106 112 130 T89-26 21 24 35 48 60 70 98 111 130 T89-27 21 24 34 48 60 70 93 106 120 T89-28 20 22 32 48 60 70 93 105 116

Table 2.41 Diameter growth data for TF0132rp1

Days in the greenhouse 33 Diameter (mm) 40 47 54 TF0132rp1-1B-1 5.1 6.3 7.4 9.1 TF0132rp1-1B-2 4.8 5.8 6.9 8.1 TF0132rp1-1B-3 4.2 5.8 7.0 7.6 TF0132rp1-3BB-1 4.0 5.5 6.7 7.4 TF0132rp1-3BB-2 5.1 6.1 6.8 8.2 TF0132rp1-3BB-3 4.9 5.7 6.8 8.4 TF0132rp1-4AC-1 4.3 6.2 8.0 9.1 TF0132rp1-4AC-2 5.0 6.6 8.5 9.4 TF0132rp1-4AC-3 4.8 6.5 7.5 8.5 TF0132rp1-4B-1 4.8 5.1 6.5 7.9 TF0132rp1-4B-2 4.8 5.3 6.3 7.2 TF0132rp1-4B-3 4.5 5.6 7.7 8.4 TF0132rp1-6B-1 4.2 5.3 6.6 6.9 TF0132rp1-6B-2 4.5 5.8 7.6 8.2 TF0132rp1-6B-3 6.5 6.7 7.3 8.4

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T89-20 4.8 6.2 6.3 6.9 T89-21 3.8 5.5 6.0 6.9 T89-22 4.5 6.2 6.6 7.5 T89-23 3.8 4.8 5.6 6.2 T89-24 3.8 4.9 5.8 7.2 T89-25 4.8 5.9 7.4 8.9 T89-26 4.4 6.2 7.0 7.8 T89-27 4.9 6.4 7.3 8.3 T89-28 4.5 4.8 5.8 7.2

[00278] The results of the growth analysis are specified in the overview of Table 2.42. The growth effects determined from the specified construction group are presented as ratios between AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC of the construction group and the wild type.

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Growth Rate of Maximum Diameter 1.02 Maximum Height Growth Rate Maximum 1.23 Diameter Maximum End 1.01 Maximum Final Height 1.28 Average Diameter Growth Rate 1.10 Maximum Height Growth Rate 1.31 Diameter Medium Fi- nal 1.07 Final Average Height 1.29 Construction group TF0132rp1

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Construction Group TF0146 [00279] This construction induces increased growth. The final height is 13% higher compared to the average of the construction group and the wild type control group. The final height is 16% higher compared to the largest individuals in the construction group and the wild type control group. The maximum growth rate is 18% higher compared to the average of the construction group and the wild type control group. The maximum height growth rate is 25% higher compared to the largest individuals in the construction group and the wild type control group. The final diameter is 8% larger compared to the average for the construction group and the wild-type control group. The diameter is 8% larger compared to the largest individuals in the construction group and the wild type control group. Construction group TF0146 satisfies the most stringent level of criteria for selecting growth differences (1) and (4) shown in Table 2.45.

[00280] Tables 2.43 and 2.44 contain growth data for the specified construction group and the corresponding wild type group. The columns in the table contain diameter measurements of individuals from the specified construction group and the corresponding wild type group. The measurement time as a number of days in the greenhouse is shown in the table headings.

Table 2.43 Height growth data for TF0146

Height (cm)

Days in the greenhouse 18 25 28 32 36 39 46 53 55 TF0146-1A 25 43 51 60 80 94 125 152 158 TF0146-1B 16 32 42 55 68 82 113 140 148 TF0146-2A 18 31 41 51 64 74 98 124 132 TF0146-2B 22 42 55 64 81 95 125 156 162

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TF0146-3A 24 38 51 63 77 89 116 139 144 TF0146-3B 18 30 38 49 64 74 95 114 122 TF0146-4A 20 34 47 58 72 84 107 124 132 TF0146-4B 24 39 52 63 79 91 120 149 158 T89-01 18 32 43 54 66 76 98 124 133 T89-02 20 33 43 53 67 76 101 124 129 T89-03 17 31 43 53 68 76 102 128 135 T89-04 19 33 44 54 67 74 97 122 129 T89-05 20 33 44 54 67 76 100 121 129 T89-06 18 31 42 53 67 76 97 121 128 T89-07 16 28 41 53 65 74 96 121 129 T89-08 19 34 43 53 65 75 98 123 131 T89-09 20 30 40 52 64 73 97 119 125 T89-10 21 33 41 49 61 70 92 114 121 T89-11 18 31 40 49 61 71 94 117 123 T89-12 18 31 40 AT 60 68 90 107 115 T89-13 19 35 47 58 70 78 103 128 135 T89-14 19 31 40 50 61 72 93 118 124 T89-15 20 32 41 50 62 70 91 114 120 T89-16 18 30 39 49 62 71 92 114 122 T89-17 17 33 45 55 70 78 104 129 136 T89-18 19 32 44 56 71 82 106 130 137 T89-19 16 26 36 46 51 69 89 112 119 T89-20 16 31 41 49 60 71 91 111 118 T89-21 20 31 44 54 68 76 99 122 127 T89-22 14 26 37 46 61 72 96 120 127 T89-23 19 32 40 50 63 73 100 121 127 T89-24 20 31 41 47 58 68 90 111 117 T89-25 20 36 45 57 69 78 101 127 134 T89-26 20 37 49 58 71 80 107 131 140 T89-27 19 34 44 55 71 81 107 131 138 T89-28 17 35 44 56 68 76 99 126 132 T89-29 17 32 45 55 68 78 101 125 132

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T89-30 18 31 41 50 63 72 95 119 125 T89-31 17 27 35 45 58 67 87 108 116 T89-32 19 32 44 52 65 74 98 121 127 T89-33 20 33 43 53 64 74 96 119 126 T89-34 19 34 44 55 68 78 100 124 130 T89-35 17 30 40 51 64 74 95 116 122 T89-36 18 30 40 49 62 71 91 114 121 T89-82 21 33 43 54 65 75 98 118 125 T89-83 22 35 47 55 71 80 105 130 137 T89-85 21 36 46 56 67 76 98 123 131

Table 2.44 Diameter growth data for TF0146

Days in the greenhouse 32 39 Diameter (mm) 46 53 55 TF0146-1A 5.4 6.2 7.2 6.9 7.5 TF0146-1B 4.2 5.7 7.0 8.3 8.6 TF0146-2A 6.1 6.4 7.4 8.7 9.4 TF0146-2B 5.5 7.0 8.5 9.9 9.9 TF0146-3A 6.0 7.1 8.1 8.3 8.3 TF0146-3B 4.2 5.6 6.8 8.5 8.4 TF0146-4A 4.8 5.1 6.9 7.2 7.2 TF0146-4B 5.5 8.8 8.5 10.0 9.1 T89-01 5.2 6.0 6.8 8.2 7.6 T89-02 4.5 5.8 7.1 8.6 8.3 T89-03 4.4 5.7 6.8 7.9 8.1 T89-04 4.4 6.0 6.9 8.6 8.5 T89-05 4.6 5.8 6.7 7.9 8.0 T89-06 4.7 5.9 7.2 8.1 9.2 T89-07 4.8 5.5 6.2 6.9 7.1 T89-08 4.5 5.4 6.0 6.9 7.1 T89-09 4.7 5.6 6.7 8.8 8.0 T89-10 4.1 4.9 5.6 7.1 7.0 T89-11 3.8 5.3 7.3 8.0 7.6 T89-12 AT 5.1 6.2 7.3 6.9

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T89-13 4.8 5.7 6.5 7.6 7.6 T89-14 4.2 5.5 6.6 7.6 6.5 T89-15 5.0 6.1 7.2 7.9 8.3 T89-16 4.4 5.6 6.2 7.3 7.9 T89-17 4.8 6.7 7.0 8.3 8.4 T89-18 4.2 5.5 6.7 7.3 7.4 T89-19 4.7 5.5 6.8 7.2 7.8 T89-20 4.5 5.6 6.5 7.0 7.3 T89-21 4.9 5.6 6.4 7.4 7.6 T89-22 4.3 5.4 6.5 7.3 7.6 T89-23 3.9 5.5 6.7 7.7 7.0 T89-24 4.3 5.9 6.8 8.1 7.9 T89-25 4.7 6.5 7.5 9.2 9.1 T89-26 5.4 5.9 7.7 8.6 8.8 T89-27 4.7 5.6 7.4 7.9 8.0 T89-28 4.7 5.7 6.3 7.2 7.4 T89-29 4.8 5.6 6.6 7.7 8.1 T89-30 4.6 5.5 6.7 7.3 7.3 T89-31 4.3 5.8 6.3 7.5 7.6 T89-32 4.6 5.9 7.6 9.3 9.1 T89-33 4.4 5.3 6.2 7.1 7.1 T89-34 4.6 5.6 6.8 8.1 8.7 T89-35 5.3 6.0 7.5 8.8 8.9 T89-36 4.4 6.1 6.9 8.1 8.6 T89-82 4.7 5.3 6.5 7.5 7.4 T89-83 4.7 6.1 7.1 8.3 8.3 T89-85 5.1 6.3 7.3 7.7 8.2

[00281] The results from the growth analysis are specified in the general Table 2.45. The determined growth effects of the specified construction group are presented as ratios between AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC of the construction group and the wild type.

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Growth Rate of Maximum Diameter 1.02 Maximum Height Growth Rate Maximum 0.95 Diameter Maximum End 1.25 Maximum Final Height 1.16 Average Diameter Growth Rate 0.99 Maximum Height Growth Rate CO Final Average Diameter 1.08 Final Average Height 1.13 Construction group TF0146

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Construction group TF0173 [00282] This construction induces increased growth. The growth rate of increased diameter is 19% higher compared to the average of the construction group and the wild control group. Construction group TF0173 satisfies the growth difference selection criterion (3) as shown in Table 2.48.

[00283] Tables 2.46 and 2.47 contain growth data for the specified construction group and the corresponding wild type group. The columns in the table contain height and diameter measurements of individuals from the specified construction group and the corresponding wild type group. The measurement time as a number of days in the greenhouse is shown in the table headings.

Table 2.46 Height growth data for TF0173

Height (cm)

Greenhouse days 22 27 31 34 38 41 45 48 52 56 TF0173-3A-1 28 39 51 60 73 81 93 104 120 134 TF0173-3A-2 24 34 44 53 65 74 87 98 111 123 TF0173-3B-1 30 42 57 67 81 94 108 120 136 155 TF0173-3B-2 26 37 50 62 76 88 103 114 131 147 TF0173-4A-1 26 37 51 60 76 87 101 112 125 141 TF0173-4A-2 28 40 54 64 77 88 102 111 125 142 TF0173-4B-1 30 41 57 65 80 91 105 115 AT 136 TF0173-4B-2 30 38 49 58 71 82 95 105 116 140 T89-01 27 37 47 58 69 79 93 100 114 129 T89-02 28 38 48 57 69 75 84 91 97 104 T89-03 26 38 49 59 69 82 94 106 122 139 T89-04 27 37 49 58 70 82 99 110 124 140 T89-05 29 40 52 60 74 83 96 106 120 134 T89-06 29 39 51 59 70 85 97 105 118 133 T89-07 24 32 43 52 61 71 81 90 96 102 T89-08 27 35 45 54 67 76 88 99 114 129 T89-09 30 40 53 64 75 86 99 110 123 136

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T89-10 26 37 48 59 73 80 92 101 115 129 T89-12 32 43 56 68 80 93 108 117 131 144 T89-13 29 41 50 61 72 82 93 104 118 136 T89-14 28 36 47 58 69 77 89 100 115 132 T89-15 24 38 48 57 68 78 93 106 120 135 T89-16 29 40 53 63 74 85 AT 109 122 137 T89-18 30 40 52 61 73 81 93 104 115 124 T89-19 30 43 56 67 80 92 108 117 132 149 T89-20 31 42 56 65 78 89 102 113 129 145 T89-21 31 42 55 64 78 89 102 116 132 147 T89-23 32 41 52 62 74 85 97 107 120 135 T89-24 25 36 47 55 69 78 92 101 113 128 T89-25 30 40 52 61 73 84 101 112 125 139 T89-26 28 40 50 60 72 85 97 109 122 136 T89-27 27 36 46 53 65 74 85 96 109 123 T89-31 30 41 51 61 73 84 98 109 123 138 T89-32 28 38 52 62 75 85 98 107 121 135 T89-35 29 40 54 64 76 88 101 110 124 139 T89-36 24 34 43 52 63 72 84 95 108 120 T89-37 27 40 52 63 73 84 97 105 122 137 T89-38 13 14 18 22 39 46 55 62 72 81 T89-39 28 39 51 60 73 85 98 106 120 134 T89-40 23 42 45 54 68 75 86 97 108 122 T89-41 29 40 53 63 75 85 99 107 117 131 T89-42 33 43 58 69 83 97 111 121 136 150 T89-45 23 36 48 58 69 78 93 103 119 AT T89-46 25 34 44 51 60 68 77 87 99 113 T89-69 28 38 49 61 75 86 100 110 126 142

Table 2.47 Diameter growth data for TF0173

Days in the greenhouse Diameter (mm) 34 45 48 56 Days in the greenhouse Diameter (mm) 34 45 48 56 TF0173-3A-1 5.6 6.4 7.3 8.0 T89-18 7.2 7.7 8.4 8.3

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TF0173-3A-2 6.5 6.8 7.3 9.2 T89-19 6.1 8.1 8.7 9.4 TF0173-3B-1 6.0 8.9 9.0 9.8 T89-20 6.8 8.0 8.2 9.7 TF0173-3B-2 5.7 8.2 8.7 9.5 T89-21 6.3 7.3 8.3 9.6 TF0173-4A-1 6.0 7.9 8.5 10.1 T89-23 5.7 7.9 7.8 9.6 TF0173-4A-2 5.6 8.0 8.5 10.7 T89-24 5.7 6.9 7.1 8.4 TF0173-4B-1 6.3 8.1 9.0 11.5 T89-25 5.7 8.0 9.2 10.3 TF0173-4B-2 5.2 6.8 8.7 9.3 T89-26 6.5 8.7 9.5 10.3 T89-01 7.0 AT 9.0 10.2 T89-27 6.4 7.7 8.6 10.1 T89-02 5.9 6.0 6.4 6.8 T89-31 5.6 8.6 7.7 9.2 T89-03 6.2 7.9 10.3 9.8 T89-32 7.2 9.0 9.2 10.6 T89-04 6.2 9.0 8.2 9.5 T89-35 6.9 8.1 8.6 9.6 T89-05 6.2 8.0 8.4 9.8 T89-36 5.3 7.4 8.2 9.7 T89-06 6.6 8.9 9.7 9.9 T89-37 5.5 8.0 8.7 9.2 T89-07 5.2 6.3 6.7 7.0 T89-38 5.4 AT 6.1 7.0 T89-08 5.7 6.9 7.9 9.1 T89-39 6.0 8.3 8.8 10.4 T89-09 4.1 7.6 9.0 9.8 T89-40 5.3 7.0 7.7 8.7 T89-10 6.3 8.5 8.4 9.9 T89-41 5.4 7.4 7.8 9.3 T89-12 5.8 7.8 8.3 9.7 T89-42 6.2 8.5 9.1 10.5 T89-13 5.7 7.8 8.0 9.3 T89-45 5.4 8.5 8.7 9.9 T89-14 6.5 6.8 7.1 8.8 T89-46 7.3 7.4 8.0 9.6 T89-15 6.0 7.6 8.3 9.5 T89-69 5.7 8.0 8.7 8.9 T89-16 6.3 8.6 7.8 9.3

[00284] The results of the growth analysis are specified in General Table 2.48. The determined growth effects of the specified construction group are shown as ratios between AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC of the construction group and the wild type.

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Φ 0

£ o £ Φ

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Growth Rate of Maximum Diameter 0.99 Maximum Height Growth Rate Maximum 1.04 Diameter Maximum End 1.09 Maximum Final Height 1.03 Average Diameter Growth Rate 1.19 Maximum Height Growth Rate 1.07 Final Average Diameter 1.04 Final Average Height 1.06 Construction group TF0173

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Construction group TF0247 [00285] This construction induces increased growth. The final height is 7% higher compared to the average of the construction group and the control group of the wild type. The final height is 10% higher compared to the larger individuals in the construction group and the wild control group. The maximum height growth rate is 5% higher compared to the average for the construction group and the wild-type control group. The maximum height growth rate is 7% higher compared to the largest individuals in the construction group and the wild control group. The final diameter is 18% larger compared to the average of the construction group and the wild group. The final diameter is 9% larger compared to the larger individuals in the construction group and the wild type control group. The growth rate in diameter is 22% higher compared to the average of the construction group and the control group of the wild type. The diameter growth rate is 18% higher compared to the largest individuals in the construction group and the wild type control group. Construction group TF0247 satisfies the most stringent level of growth difference selection criteria (2) and the least stringent level of growth difference selection criteria (1), (3) and (4) shown in Table 2.51.

[00286] Tables 2.49 and 2.50 contain growth data for the specified construction group and the corresponding wild type group. The columns in the Tables contain measurements of height and diameter of individuals from the specified construction group and the corresponding wild type group. The measurement time as a number of days in the greenhouse is shown in the table headings.

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Table 2.49 Height growth data for TF0247

Height (cm)

Days in the greenhouse 18 25 28 32 36 39 46 53 55 TF0247-1A 18 29 40 52 67 76 98 123 131 TF0247-3A 22 38 51 63 78 90 118 145 154 TF0247-3B 23 37 50 61 76 87 114 139 145 TF0247-4A 17 30 41 53 68 77 103 127 134 TF0247-6B 25 40 52 63 73 83 97 111 116 T89-01 18 32 43 54 66 76 98 124 133 T89-02 20 33 43 53 67 76 101 124 129 T89-03 17 31 43 53 68 76 102 128 135 T89-04 19 33 44 54 67 74 97 122 129 T89-05 20 33 44 54 67 76 100 121 129 T89-06 18 31 42 53 67 76 97 121 128 T89-07 16 28 41 53 65 74 96 121 129 T89-08 19 34 43 53 65 75 98 123 131 T89-09 20 30 40 52 64 73 97 119 125 T89-10 21 33 41 49 61 70 92 114 121 T89-11 18 31 40 49 61 71 94 117 123 T89-12 18 31 40 AT 60 68 90 107 115 T89-13 19 35 47 58 70 78 103 128 135 T89-14 19 31 40 50 61 72 93 118 124 T89-15 20 32 41 50 62 70 91 114 120 T89-16 18 30 39 49 62 71 92 114 122 T89-17 17 33 45 55 70 78 104 129 136 T89-18 19 32 44 56 71 82 106 130 137 T89-19 16 26 36 46 51 69 89 112 119 T89-20 16 31 41 49 60 71 91 111 118 T89-21 20 31 44 54 68 76 99 122 127 T89-22 14 26 37 46 61 72 96 120 127 T89-23 19 32 40 50 63 73 100 121 127 T89-24 20 31 41 47 58 68 90 111 117 T89-25 20 36 45 57 69 78 101 127 134

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T89-26 20 37 49 58 71 80 107 131 140 T89-27 19 34 44 55 71 81 107 131 138 T89-28 17 35 44 56 68 76 99 126 132 T89-29 17 32 45 55 68 78 101 125 132 T89-30 18 31 41 50 63 72 95 119 125 T89-31 17 27 35 45 58 67 87 108 116 T89-32 19 32 44 52 65 74 98 121 127 T89-33 20 33 43 53 64 74 96 119 126 T89-34 19 34 44 55 68 78 100 124 130 T89-35 17 30 40 51 64 74 95 116 122 T89-36 18 30 40 49 62 71 91 114 121 T89-82 21 33 43 54 65 75 98 118 125 T89-83 22 35 47 55 71 80 105 130 137 T89-85 21 36 46 56 67 76 98 123 131

Table 2.50 Diameter growth data for TF0247

Days in the greenhouse 32 39 Diameter (mm) 46 53 55 TF0247-1A 4.9 6.0 7.4 8.5 9.7 TF0247-3A 5.8 6.8 7.6 8.7 9.1 TF0247-3B 5.4 7.0 8.1 9.5 10.0 TF0247-4A 4.6 5.7 7.4 10.3 9.7 TF0247-6B 6.0 6.8 7.0 7.3 7.9 T89-01 5.2 6.0 6.8 8.2 7.6 T89-02 4.5 5.8 7.1 8.6 8.3 T89-03 4.4 5.7 6.8 7.9 8.1 T89-04 4.4 6.0 6.9 8.6 8.5 T89-05 4.6 5.8 6.7 7.9 8.0 T89-06 4.7 5.9 7.2 8.1 9.2 T89-07 4.8 5.5 6.2 6.9 7.1 T89-08 4.5 5.4 6.0 6.9 7.1 T89-09 4.7 5.6 6.7 8.8 8.0 T89-10 4.1 4.9 5.6 7.1 7.0 T89-11 3.8 5.3 7.3 8.0 7.6

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T89-12 AT 5.1 6.2 7.3 6.9 T89-13 4.8 5.7 6.5 7.6 7.6 T89-14 4.2 5.5 6.6 7.6 6.5 T89-15 5.0 6.1 7.2 7.9 8.3 T89-16 4.4 5.6 6.2 7.3 7.9 T89-17 4.8 6.7 7.0 8.3 8.4 T89-18 4.2 5.5 6.7 7.3 7.4 T89-19 4.7 5.5 6.8 7.2 7.8 T89-20 4.5 5.6 6.5 7.0 7.3 T89-21 4.9 5.6 6.4 7.4 7.6 T89-22 4.3 5.4 6.5 7.3 7.6 T89-23 3.9 5.5 6.7 7.7 7.0 T89-24 4.3 5.9 6.8 8.1 7.9 T89-25 4.7 6.5 7.5 9.2 9.1 T89-26 5.4 5.9 7.7 8.6 8.8 T89-27 4.7 5.6 7.4 7.9 8.0 T89-28 4.7 5.7 6.3 7.2 7.4 T89-29 4.8 5.6 6.6 7.7 8.1 T89-30 4.6 5.5 6.7 7.3 7.3 T89-31 4.3 5.8 6.3 7.5 7.6 T89-32 4.6 5.9 7.6 9.3 9.1 T89-33 4.4 5.3 6.2 7.1 7.1 T89-34 4.6 5.6 6.8 8.1 8.7 T89-35 5.3 6.0 7.5 8.8 8.9 T89-36 4.4 6.1 6.9 8.1 8.6 T89-82 4.7 5.3 6.5 7.5 7.4 T89-83 4.7 6.1 7.1 8.3 8.3 T89-85 5.1 6.3 7.3 7.7 8.2

[00287] The results of the growth analysis are specified in General Table 2.51. The determined growth effects of the specified construction group are shown as ratios between AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC of the construction group and the wild type.

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Growth Rate of Maximum Diameter CO Maximum Height Growth Rate Maximum 1.07 Diameter Maximum End 1.09 Maximum Final Height 1.10 Average Diameter Growth Rate 1.22 Maximum Height Growth Rate 1.05 Final Average Diameter CO Final Average Height 1.07 Construction group TF0247

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Construction group TF0405 [00288] This construction induces increased growth. The final height is 10% higher compared to the average of the construction group and the control group of the wild type. The final height is 9% higher compared to the larger individuals in the construction group and the wild control group. The maximum height growth rate is 13% higher compared to the average for the construction group and the wild-type control group. The maximum height growth rate is 15% higher compared to the largest individuals in the building group and the wild control group. The final diameter is 15% larger compared to the average of the construction group and the wild group. The final diameter is 10% larger compared to the larger individuals in the construction group and the wild type control group. The diameter growth rate is 22% higher compared to the average of the construction group and the wild type control group. The diameter growth rate is 19% higher compared to the largest individuals in the construction group and the wild type control group. Construction group TF0405 satisfies the most stringent level of growth difference selection criteria (2) and the least stringent level of growth difference selection criteria (1) and (2) and the least stringent level of difference criteria (3) and (4) as shown in Table 2.54.

[00289] Tables 2.52 and 2.53 contain growth data for the specified construction group and the corresponding wild type group. The columns in the Tables contain measurements of height and diameter of individuals from the specified construction group and the corresponding wild type group. The measurement time as a number of days in the greenhouse is shown in the table headings.

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Table 2.52 Height growth data for TF0405

Height (cm)

Days in the greenhouse 18 25 28 32 36 39 46 53 55 TF0405-2A-1 7 17 25 36 46 57 83 109 117 TF0405-2A-2 21 36 50 60 74 87 118 146 153 TF0405-2B-2 19 34 47 58 73 84 108 130 136 TF0405-3A-1 24 41 54 63 77 88 117 144 150 TF0405-3A-2 25 38 52 64 79 91 117 142 150 TF0405-3B-1 19 33 44 54 65 75 98 123 130 TF0405-3B-2 22 36 49 59 72 86 112 136 145 T89-01 18 32 43 54 66 76 98 124 133 T89-02 20 33 43 53 67 76 101 124 129 T89-03 17 31 43 53 68 76 102 128 135 T89-04 19 33 44 54 67 74 97 122 129 T89-05 20 33 44 54 67 76 100 121 129 T89-06 18 31 42 53 67 76 97 121 128 T89-07 16 28 41 53 65 74 96 121 129 T89-08 19 34 43 53 65 75 98 123 131 T89-09 20 30 40 52 64 73 97 119 125 T89-10 21 33 41 49 61 70 92 114 121 T89-11 18 31 40 49 61 71 94 117 123 T89-12 18 31 40 AT 60 68 90 107 115 T89-13 19 35 47 58 70 78 103 128 135 T89-14 19 31 40 50 61 72 93 118 124 T89-15 20 32 41 50 62 70 91 114 120 T89-16 18 30 39 49 62 71 92 114 122 T89-17 17 33 45 55 70 78 104 129 136 T89-18 19 32 44 56 71 82 106 130 137 T89-19 16 26 36 46 51 69 89 112 119 T89-20 16 31 41 49 60 71 91 111 118 T89-21 20 31 44 54 68 76 99 122 127 T89-22 14 26 37 46 61 72 96 120 127 T89-23 19 32 40 50 63 73 100 121 127 T89-24 20 31 41 47 58 68 90 111 117 T89-25 20 36 45 57 69 78 101 127 134 T89-26 20 37 49 58 71 80 107 131 140 T89-27 19 34 44 55 71 81 107 131 138 T89-28 17 35 44 56 68 76 99 126 132 T89-29 17 32 45 55 68 78 101 125 132

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T89-30 18 31 41 50 63 72 95 119 125 T89-31 17 27 35 45 58 67 87 108 116 T89-32 19 32 44 52 65 74 98 121 127 T89-33 20 33 43 53 64 74 96 119 126 T89-34 19 34 44 55 68 78 100 124 130 T89-35 17 30 40 51 64 74 95 116 122 T89-36 18 30 40 49 62 71 91 114 121 T89-82 21 33 43 54 65 75 98 118 125 T89-83 22 35 47 55 71 80 105 130 137 T89-85 21 36 46 56 67 76 98 123 131

Table 2.53 Diameter growth data for TF0405

Days in the greenhouse 32 39 Diameter (mm) 46 53 55 TF0405-2A-1 4.0 5.1 6.8 9.1 9.2 TF0405-2A-2 5.3 6.9 8.3 10.2 10.1 TF0405-2B-2 5.3 7.0 7.7 8.9 9.0 TF0405-3A-1 5.2 6.9 8.1 9.1 8.9 TF0405-3A-2 5.4 6.7 8.0 9.2 9.0 TF0405-3B-1 5.1 5.8 7.3 7.8 7.9 TF0405-3B-2 5.3 6.3 7.8 9.2 9.1 T89-01 5.2 6.0 6.8 8.2 7.6 T89-02 4.5 5.8 7.1 8.6 8.3 T89-03 4.4 5.7 6.8 7.9 8.1 T89-04 4.4 6.0 6.9 8.6 8.5 T89-05 4.6 5.8 6.7 7.9 8.0 T89-06 4.7 5.9 7.2 8.1 9.2 T89-07 4.8 5.5 6.2 6.9 7.1 T89-08 4.5 5.4 6.0 6.9 7.1 T89-09 4.7 5.6 6.7 8.8 8.0 T89-10 4.1 4.9 5.6 7.1 7.0 T89-11 3.8 5.3 7.3 8.0 7.6 T89-12 AT 5.1 6.2 7.3 6.9 T89-13 4.8 5.7 6.5 7.6 7.6 T89-14 4.2 5.5 6.6 7.6 6.5 T89-15 5.0 6.1 7.2 7.9 8.3 T89-16 4.4 5.6 6.2 7.3 7.9 T89-17 4.8 6.7 7.0 8.3 8.4 T89-18 4.2 5.5 6.7 7.3 7.4 T89-19 4.7 5.5 6.8 7.2 7.8

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T89-20 4.5 5.6 6.5 7.0 7.3 T89-21 4.9 5.6 6.4 7.4 7.6 T89-22 4.3 5.4 6.5 7.3 7.6 T89-23 3.9 5.5 6.7 7.7 7.0 T89-24 4.3 5.9 6.8 8.1 7.9 T89-25 4.7 6.5 7.5 9.2 9.1 T89-26 5.4 5.9 7.7 8.6 8.8 T89-27 4.7 5.6 7.4 7.9 8.0 T89-28 4.7 5.7 6.3 7.2 7.4 T89-29 4.8 5.6 6.6 7.7 8.1 T89-30 4.6 5.5 6.7 7.3 7.3 T89-31 4.3 5.8 6.3 7.5 7.6 T89-32 4.6 5.9 7.6 9.3 9.1 T89-33 4.4 5.3 6.2 7.1 7.1 T89-34 4.6 5.6 6.8 8.1 8.7 T89-35 5.3 6.0 7.5 8.8 8.9 T89-36 4.4 6.1 6.9 8.1 8.6 T89-82 4.7 5.3 6.5 7.5 7.4 T89-83 4.7 6.1 7.1 8.3 8.3 T89-85 5.1 6.3 7.3 7.7 8.2

[00290] The results of the growth analysis are specified in General Table 2.51. The determined growth effects of the specified construction group are shown as ratios between AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC of the construction group and the wild type.

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Growth Rate of Maximum Diameter 1.19 Maximum Height Growth Rate Maximum 1.15 Diameter Maximum End 1.10 Maximum Final Height 1.09 Average Diameter Growth Rate 1.22 Maximum Height Growth Rate 1.13 Final Average Diameter 1.15 Final Average Height 1.10 Construction group TF0405

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Construction group TFSTT004 [00291] Tables 2.55 and 2.56 contain growth data for the specified construction group and the corresponding wild type group. The columns in the tables contain height and diameter measurements of the individuals in the specified construction group and the corresponding wild type group. The measurement time as a number of days in the greenhouse is shown in the table headings.

Table 2.55 Height growth data for TFSTT004

Days in the greenhouse 19 28 34 40 Height (cm) 54 61 65 44 47 51 TFSTT004-1A 18 35 55 71 84 95 105 114 135 145 TFSTT004-2A-1 16 33 54 67 82 89 100 108 130 141 TFSTT004-2A-2 19 39 57 71 84 94 106 112 130 140 TFSTT004-2B-1 20 41 61 78 90 99 110 119 139 150 TFSTT004-2B-2 20 38 59 74 85 93 103 113 135 146 TFSTT004-3B 19 35 53 66 77 87 98 107 124 134 TFSTT004-4B-1 18 33 51 63 76 86 98 108 129 142 TFSTT004-4B-2 18 35 57 74 86 96 108 116 141 152 T89-01 18 30 46 58 69 77 87 96 113 122 T89-02 18 30 49 62 72 77 84 90 102 109 T89-03 15 27 41 54 65 73 82 91 112 123 T89-10 AT AT AT AT AT AT AT AT AT AT T89-11 19 31 49 61 74 83 94 103 124 133 T89-12 17 30 45 58 69 77 90 100 123 134 T89-13 18 27 43 56 68 78 91 100 121 133 T89-14 5 26 42 56 67 74 83 90 109 119 T89-15 10 15 25 33 41 45 52 57 72 82 T89-16 19 33 53 63 73 82 93 102 119 130 T89-17 17 29 46 58 66 72 80 86 96 102 T89-18 16 30 48 59 71 81 93 103 122 132 T89-19 18 32 50 65 77 84 94 102 126 139 T89-20 16 33 52 67 79 88 98 105 124 139

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T89-21 17 29 44 58 67 73 83 90 110 122 T89-22 14 28 47 61 71 80 89 98 119 133 T89-23 10 33 45 53 63 71 82 90 112 123 T89-24 20 28 43 51 62 69 78 87 106 119 T89-25 14 26 38 52 64 72 82 89 110 122 T89-26 15 28 44 57 69 77 87 96 120 133 T89-27 18 29 47 62 75 82 92 103 125 138 T89-28 22 37 54 67 78 88 97 104 123 133 T89-29 16 33 49 63 76 84 93 100 123 138 T89-30 15 40 46 58 67 74 85 92 113 124

Table 2.56 Diameter growth data for TFSTT004

Days in the greenhouse Diameter (mm) 28 34 40 44 47 51 54 61 65 TFSTT004-1A 4.6 6.0 8.5 7.9 8.6 9.0 10.3 11.2 11.3 TFSTT004-2A-1 4.6 5.7 6.5 7.4 8.8 9.1 9.8 10.6 10.7 TFSTT004-2A-2 4.2 5.9 6.9 7.5 8.5 8.9 9.6 10.2 10.3 TFSTT004-2B-1 5.0 6.5 7.8 8.9 9.6 10.9 10.9 12.1 12.6 TFSTT004-2B-2 4.5 6.2 7.3 8.3 8.3 9.3 8.9 10.7 11.0 TFSTT004-3B 4.1 5.6 6.8 7.5 8.2 9.0 9.9 10.6 10.7 TFSTT004-4B-1 3.6 5.0 6.0 7.0 7.5 9.3 9.2 10.6 11.0 TFSTT004-4B-2 4.2 5.7 7.5 8.6 9.2 9.5 10.4 11.1 11.7 T89-01 3.2 4.6 5.4 6.2 6.8 7.9 8.3 9.5 9.3 T89-02 3.4 4.7 5.5 7.3 6.3 6.6 6.9 8.3 7.5 T89-03 3.9 4.4 5.2 6.2 6.4 7.8 7.6 9.7 9.4 T89-10 AT AT AT AT AT AT AT AT AT T89-11 3.4 5.1 6.2 7.6 6.8 6.9 7.7 10.1 9.5 T89-12 2.9 4.7 5.8 6.6 7.8 8.0 8.7 9.1 9.2 T89-13 3.0 4.3 5.4 5.8 6.4 7.6 7.8 8.2 8.8 T89-14 3.0 4.5 5.7 6.3 6.7 7.9 7.8 9.0 8.9 T89-15 AT 2.1 3.0 4.0 4.0 4.5 4.9 5.5 5.6 T89-16 3.5 5.3 6.4 6.6 7.0 7.2 8.3 8.2 9.0

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T89-17 3.4 4.6 5.1 5.4 6.0 6.4 6.5 6.8 7.1 T89-18 3.6 5.2 6.0 7.0 7.8 8.2 9.9 10.3 9.7 T89-19 4.2 5.5 6.6 7.7 8.5 8.9 9.5 11.1 12.3 T89-20 4.1 5.5 6.6 8.1 9.3 9.6 9.3 10.0 11.1 T89-21 3.1 5.6 5.8 6.7 7.1 7.8 8.4 9.7 10.1 T89-22 3.2 4.4 5.6 6.5 7.5 7.6 7.8 8.9 9.2 T89-23 2.4 4.2 5.1 6.1 6.5 7.5 10.1 9.3 10.2 T89-24 3.2 4.5 5.1 6.3 7.0 7.6 8.1 8.8 9.1 T89-25 3.3 4.3 5.2 5.8 6.5 7.4 7.8 9.3 9.7 T89-26 3.3 4.4 5.5 6.6 7.2 8.1 8.9 9.5 10.5 T89-27 3.3 4.9 6.0 7.8 8.0 8.9 9.7 11.2 11.5 T89-28 4.5 5.7 7.4 7.8 8.5 9.4 9.7 10.2 11.1 T89-29 3.1 4.7 6.3 7.2 7.9 9.2 9.8 11.1 10.7 T89-30 3.0 5.8 6.2 7.7 7.9 8.6 8.2 10.1 10.3

[00292] The results of the growth analysis are specified in General Table 2.57. The determined growth effects of the specified construction group are shown as ratios between AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC of the construction group and the wild type.

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Growth Rate of Maximum Diameter 0.91 Maximum Height Growth Rate Maximum 0.97 Diameter Maximum End 1.02 Maximum Final Height 1.09 Average Diameter Growth Rate 1.09 Maximum Height Growth Rate 1.06 Final Average Diameter 1.17 Final Average Height 1.15 Construction group TFSTT004

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Construction Group TFSTT013 [00293] The overexpressed gene with the TFSTT013 construct generates the same top hits as the overexpressed gene with the TFSTT038 construct, when using the BLAST search against the Jamboree Gene Model database of P. tríchocarpa on the web page from the Joint Genome Institute (http://genome.jgi-psf.org/cgi- bin / runAlignment? db = Poptr1 1), indicating high homology between the two genes.

[00294] Tables 2.58 and 2.59 contain growth data for the specified construction group and the corresponding wild type group. The columns in the tables contain height and diameter measurements of the individuals in the specified construction group and the corresponding wild type group. The measurement time as a number of days in the greenhouse is shown in the table headings.

Table 2.58 Height growth data for TFSTT013

Days in the greenhouse 19 25 32 Height (cm) 39 48 54 56 TFSTT013-1A 18 30 54 81 113 137 140 TFSTT013-1B 19 31 54 75 108 124 127 TFSTT013-2B 16 28 48 67 98 115 121 TFSTT013-3A 17 29 48 70 89 103 110 TFSTT013-3B 20 32 53 75 106 128 136 TFSTT013-4A 23 37 59 83 116 141 149 TFSTT013-4B 21 33 57 85 120 140 147 TFSTT013-5B 18 30 52 77 109 126 133 T89-01 18 28 49 70 100 126 133 T89-02 19 29 51 76 105 128 136 T89-03 11 18 36 47 76 93 98 T89-04 16 25 48 74 102 126 133 T89-05 17 29 49 73 106 128 135 T89-06 16 28 51 74 105 127 132

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T89-07 19 28 51 73 104 125 136 T89-08 19 31 52 77 110 130 137 T89-09 17 26 44 67 93 114 122 T89-10 16 25 44 63 89 108 115 T89-11 20 31 47 68 87 114 118 T89-12 17 28 48 70 101 117 124 T89-13 18 30 52 73 105 125 130 T89-14 19 29 44 67 96 116 123 T89-15 17 29 49 72 102 124 131 T89-16 18 29 50 74 108 129 135 T89-17 14 23 43 65 93 116 123 T89-18 15 26 48 72 103 128 136 T89-19 11 19 38 63 93 115 122 T89-20 19 30 52 75 104 126 133 T89-21 17 28 48 72 104 128 135 T89-22 19 29 49 73 105 123 129 T89-23 21 30 54 79 112 135 138 T89-24 19 24 37 54 77 100 103 T89-25 15 27 46 69 98 117 123 T89-26 19 29 50 73 103 127 135 T89-27 15 27 47 70 106 129 135 T89-28 20 33 55 80 109 130 137 T89-29 24 38 62 88 122 143 151 T89-30 21 33 56 78 113 132 138 T89-31 21 31 52 76 105 126 133 T89-32 18 29 50 73 105 129 138 T89-33 23 37 61 89 121 143 150 T89-34 19 30 52 78 108 130 138 T89-35 19 31 55 79 111 134 142 T89-36 18 29 52 75 105 127 134 T89-37 22 32 52 73 103 124 130 T89-38 18 29 51 74 105 128 134 T89-39 21 31 51 72 102 119 124

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T89-40 20 32 51 74 99 121 128 T89-41 19 29 50 73 108 130 135 T89-42 15 26 46 68 95 113 119 T89-43 16 28 48 67 93 113 119 T89-44 21 31 50 70 97 116 122 T89-45 16 26 48 72 101 123 131 T89-46 21 29 50 75 106 129 138 T89-47 9 16 34 55 82 106 114 T89-48 5 8 17 24 42 52 56 T89-49 20 30 50 71 96 117 125 T89-50 16 28 50 74 105 128 136 T89-51 19 29 51 75 107 128 136 T89-52 20 29 50 75 104 124 131 T89-53 21 32 50 72 101 118 125 T89-54 21 32 55 81 112 133 142 T89-55 21 32 56 83 112 133 141

Table 2.59 Diameter growth data for TFSTT013

Days in the greenhouse Diameter (mm) 56 Days in greenhouse Diameter (mm) 39 48 54 39 48 54 56 TFSTT013-1A 5.9 7.9 8.6 9.4 T89-24 3.1 4.6 5.0 4.5 TFSTT013-1B 5.9 8.4 8.5 8.6 T89-25 5.9 7.7 8.5 8.9 TFSTT013-2B 5.7 5.6 7.8 8.1 T89-26 6.2 7.8 8.6 9.5 TFSTT013-3A 6.4 6.9 7.5 7.8 T89-27 6.1 7.6 8.2 8.6 TFSTT013-3B 5.9 7.9 8.8 9.8 T89-28 6.9 9.0 10.7 10.5 TFSTT013-4A 6.8 8.3 9.0 9.9 T89-29 7.8 8.4 10.3 10.2 TFSTT013-4B 7.2 9.1 10.4 10.1 T89-30 6.4 8.5 9.4 9.9 TFSTT013-5B 7.5 9.0 10.3 9.5 T89-31 6.8 8.6 8.8 9.5 T89-01 6.6 8.4 8.5 9.2 T89-32 6.7 8.4 10.2 10.9 T89-02 6.8 8.3 9.4 10.1 T89-33 7.1 8.7 9.4 11.4 T89-03 4.6 4.9 6.1 6.1 T89-34 7.1 8.5 11.1 10.9 T89-04 6.9 8.7 10.0 9.4 T89-35 6.7 9.0 8.9 11.4 T89-05 5.6 7.6 8.2 8.8 T89-36 6.2 8.0 8.5 8.5 T89-06 6.9 8.2 9.6 9.3 T89-37 5.6 7.1 7.8 8.7

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T89-07 6.6 7.9 9.5 9.5 T89-38 5.8 7.8 8.8 8.7 T89-08 6.9 8.9 9.6 9.8 T89-39 6.2 8.1 9.2 9.3 T89-09 6.1 7.6 8.6 8.7 T89-40 6.2 7.9 9.6 9.5 T89-10 5.5 7.5 8.6 8.6 T89-41 5.9 8.2 8.9 9.5 T89-11 7.4 8.4 9.3 8.7 T89-42 6.8 8.4 9.1 10.4 T89-12 5.9 7.4 8.7 9.5 T89-43 6.7 8.5 9.7 9.3 T89-13 6.2 7.7 9.2 9.5 T89-44 7.1 9.1 9.2 9.4 T89-14 5.9 7.8 9.2 8.7 T89-45 6.6 7.8 9.2 9.2 T89-15 6.8 8.1 9.4 9.7 T89-46 6.8 9.3 10.2 10.0 T89-16 7.3 9.2 10.6 10.3 T89-47 5.3 7.0 7.9 8.4 T89-17 6.4 8.0 8.9 9.1 T89-48 2.3 3.0 3.2 3.5 T89-18 7.0 7.5 8.1 8.8 T89-49 7.5 8.7 9.1 9.8 T89-19 5.6 7.4 8.6 8.9 T89-50 6.8 8.6 9.3 8.9 T89-20 6.5 8.3 8.6 9.5 T89-51 7.2 8.3 9.2 9.1 T89-21 6.2 8.1 8.0 8.7 T89-52 7.5 8.8 9.4 9.7 T89-22 6.2 8.5 9.3 10.1 T89-53 7.3 9.3 9.5 10.2 T89-23 6.2 8.1 9.2 9.5 T89-54 7.0 9.0 9.2 9.6 T89-24 3.1 4.6 5.0 4.5 T89-55 7.0 8.9 9.2 9.8

[00295] The results of the growth analysis are specified in General Table 2.60. The determined growth effects of the specified construction group are shown as ratios between AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC of the construction group and the wild type.

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Growth Rate of Maximum Diameter CO o Maximum Height Growth Rate Maximum 1.01 Diameter Maximum End 00 00 o Maximum Final Height 0.99 Average Diameter Growth Rate 0.96 Maximum Height Growth Rate 1.01 Final Average Diameter 0.99 Final Average Height 1.03 Construction group TFSTT013

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Construction Group TFSTT016 [00296] Tables 2.61 and 2.62 contain growth data for the specified construction group and the corresponding wild type group. The columns in the tables contain height and diameter measurements of individuals from the specified construction group and the corresponding wild type group. The measurement time as the number of days in the greenhouse is shown in the table headings.

Table 2.61 Height growth data for TFSTT016

Days in the greenhouse 19 28 34 40 Height (cm) 54 61 65 44 47 51 TFSTT016-1A 19 37 57 71 83 91 101 111 127 137 TFSTT016-1B 19 36 54 67 78 87 98 107 126 138 TFSTT016-2A 18 34 51 67 76 84 93 101 118 130 TFSTT016-2B 18 34 55 70 80 87 97 105 125 137 TFSTT016-3A-1 19 34 51 65 76 84 95 106 125 137 TFSTT016-3A-2 17 33 50 64 76 85 96 107 129 143 TFSTT016-4A 16 33 52 69 83 91 103 112 136 151 T89-01 18 30 46 58 69 77 87 96 113 122 T89-02 18 30 49 62 72 77 84 90 102 109 T89-03 15 27 41 54 65 73 82 91 112 123 T89-10 AT AT AT AT AT AT AT AT AT AT T89-11 19 31 49 61 74 83 94 103 124 133 T89-12 17 30 45 58 69 77 90 100 123 134 T89-13 18 27 43 56 68 78 91 100 121 133 T89-14 5 26 42 56 67 74 83 90 109 119 T89-15 10 15 25 33 41 45 52 57 72 82 T89-16 19 33 53 63 73 82 93 102 119 130 T89-17 17 29 46 58 66 72 80 86 96 102 T89-18 16 30 48 59 71 81 93 103 122 132 T89-19 18 32 50 65 77 84 94 102 126 139 T89-20 16 33 52 67 79 88 98 105 124 139 T89-21 17 29 44 58 67 73 83 90 110 122

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T89-22 14 28 47 61 71 80 89 98 119 133 T89-23 10 33 45 53 63 71 82 90 112 123 T89-24 20 28 43 51 62 69 78 87 106 119 T89-25 14 26 38 52 64 72 82 89 110 122 T89-26 15 28 44 57 69 77 87 96 120 133 T89-27 18 29 47 62 75 82 92 103 125 138 T89-28 22 37 54 67 78 88 97 104 123 133 T89-29 16 33 49 63 76 84 93 100 123 138 T89-30 15 40 46 58 67 74 85 92 113 124

Table 2.62 Diameter growth data for TFSTT016

Days in the greenhouse 28 34 40 Diameter (mm) 44 47 51 54 61 65 TFSTT016-1A 3.9 5.8 7.3 8.5 8.1 9.4 9.6 9.9 10.7 TFSTT016-1B 4.1 5.7 6.4 7.2 8.3 9.1 9.3 10.9 11.1 TFSTT016-2A 3.6 5.5 6.4 7.2 8.3 8.6 9.2 10.0 10.5 TFSTT016-2B 4.3 5.7 7.0 7.5 7.8 7.9 8.6 8.9 9.1 TFSTT016-3A-1 3.8 5.6 6.7 7.8 8.9 9.9 10.7 11.4 11.7 TFSTT016-3A-2 3.6 5.0 6.5 7.7 7.8 9.3 9.1 10.4 10.9 TFSTT016-4A 4.2 5.2 6.6 8.0 8.6 9.7 9.7 11.3 11.5 T89-01 3.2 4.6 5.4 6.2 6.8 7.9 8.3 9.5 9.3 T89-02 3.4 4.7 5.5 7.3 6.3 6.6 6.9 8.3 7.5 T89-03 3.9 4.4 5.2 6.2 6.4 7.8 7.6 9.7 9.4 T89-10 AT AT AT AT AT AT AT AT AT T89-11 3.4 5.1 6.2 7.6 6.8 6.9 7.7 10.1 9.5 T89-12 2.9 4.7 5.8 6.6 7.8 8.0 8.7 9.1 9.2 T89-13 3.0 4.3 5.4 5.8 6.4 7.6 7.8 8.2 8.8 T89-14 3.0 4.5 5.7 6.3 6.7 7.9 7.8 9.0 8.9 T89-15 AT 2.1 3.0 4.0 4.0 4.5 4.9 5.5 5.6 T89-16 3.5 5.3 6.4 6.6 7.0 7.2 8.3 8.2 9.0 T89-17 3.4 4.6 5.1 5.4 6.0 6.4 6.5 6.8 7.1 T89-18 3.6 5.2 6.0 7.0 7.8 8.2 9.9 10.3 9.7 T89-19 4.2 5.5 6.6 7.7 8.5 8.9 9.5 11.1 12.3 T89-20 4.1 5.5 6.6 8.1 9.3 9.6 9.3 10.0 11.1

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T89-21 3.1 5.6 5.8 6.7 7.1 7.8 8.4 9.7 10.1 T89-22 3.2 4.4 5.6 6.5 7.5 7.6 7.8 8.9 9.2 T89-23 2.4 4.2 5.1 6.1 6.5 7.5 10.1 9.3 10.2 T89-24 3.2 4.5 5.1 6.3 7.0 7.6 8.1 8.8 9.1 T89-25 3.3 4.3 5.2 5.8 6.5 7.4 7.8 9.3 9.7 T89-26 3.3 4.4 5.5 6.6 7.2 8.1 8.9 9.5 10.5 T89-27 3.3 4.9 6.0 7.8 8.0 8.9 9.7 11.2 11.5 T89-28 4.5 5.7 7.4 7.8 8.5 9.4 9.7 10.2 11.1 T89-29 3.1 4.7 6.3 7.2 7.9 9.2 9.8 11.1 10.7 T89-30 3.0 5.8 6.2 7.7 7.9 8.6 8.2 10.1 10.3

[00297] The results of the growth analysis are specified in General Table 2.63. The determined growth effects of the specified construction group are shown as ratios between AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC of the construction group and the wild type.

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Growth Rate of Maximum Diameter 0.96 Maximum Height Growth Rate Maximum 1.04 Diameter Maximum End 0.95 Maximum Final Height 1.09 Average Diameter Growth Rate 1.09 Maximum Height Growth Rate 1.03 Final Average Diameter 1.13 Final Average Height 1.11 Construction group TFSTT016

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Construction Group TFSTT019Rp1 [00298] Tables 2.64 and 2.65 contain growth data for the specified construction group and the corresponding wild type group. The columns in the tables contain height and diameter measurements of individuals from the specified construction group and the corresponding wild type group. The measurement time as a number of days in the greenhouse is shown in the table headings.

Table 2.64 Height growth data for TFSTT019Rp1

Days in the greenhouse 20 25 29 35 Height (cm) 41 46 50 53 57 60 TFSTT019rp1-2A-1 17 24 32 45 58 71 81 88 98 104 TFSTT019rp1-2A-2 15 23 30 42 57 72 80 88 99 108 TFSTT019rp1-2A-3 16 22 28 40 55 67 78 87 97 105 TFSTT019rp1-3A-1 16 24 32 47 62 79 87 96 104 111 TFSTT019rp1-3A-2 14 20 24 28 38 54 61 69 79 85 TFSTT019rp1-3A-3 18 30 35 47 62 79 89 97 107 116 TFSTT019rp1-4BA-1 18 23 29 40 54 66 78 86 96 104 TFSTT019rp1-4BA-2 19 27 35 48 65 79 92 101 112 121 TFSTT019rp1-4BA-3 AT AT AT AT AT AT AT AT AT AT TFSTT019rp1-4BB-1 18 27 35 50 65 80 90 99 111 117 TFSTT019rp1-4BB-2 17 23 31 43 57 70 80 86 96 104 TFSTT019rp1-4BB-3 17 23 30 42 60 74 82 90 98 107 T89-01 17 26 31 45 61 76 87 95 107 116 T89-02 16 21 26 38 53 67 76 83 AT AT T89-03 18 24 32 47 64 78 87 96 106 115 T89-04 18 26 35 50 66 80 87 96 107 115 T89-05 17 26 33 44 57 73 81 91 100 107 T89-06 16 21 28 40 55 74 79 89 99 106 T89-07 17 23 31 43 57 71 80 88 98 107 T89-08 15 20 26 37 51 65 75 84 94 100 T89-09 18 25 32 46 61 74 85 93 103 112 T89-10 19 25 32 46 60 75 86 95 104 112 T89-11 20 27 36 51 68 86 98 107 120 127 T89-12 19 27 36 50 67 80 89 97 108 118 T89-13 18 25 32 45 59 75 83 92 101 108 T89-14 18 23 26 36 51 65 72 79 89 96

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T89-15 17 21 28 39 53 70 78 87 97 105 T89-16 19 25 32 43 57 71 81 90 101 109 T89-17 16 20 29 41 54 68 78 84 93 101 T89-18 16 23 30 45 63 78 87 94 103 110 T89-19 16 22 28 42 56 69 79 89 100 107 T89-20 18 25 32 47 63 78 89 99 109 115 T89-21 19 27 34 50 67 82 92 102 111 120 T89-22 19 25 32 44 58 76 84 93 102 109 T89-23 18 26 33 47 63 79 88 99 108 116 T89-24 16 24 28 42 53 70 78 85 94 103 T89-25 16 25 32 45 61 76 85 95 107 114 T89-26 16 20 26 38 52 68 78 86 97 103 T89-27 16 21 25 35 48 61 70 78 AT 98 T89-28 14 18 25 38 51 64 73 81 90 96 T89-29 7 13 18 30 45 60 69 77 90 98 T89-30 15 22 29 42 55 69 80 88 97 106 T89-31 21 29 37 50 65 80 88 98 108 116 T89-32 19 24 32 42 55 70 78 87 96 105

Table 2.65 Diameter growth data for TFSTT019Rp1

Days in the greenhouse 35 41 Diameter (mm) 46 53 60 TFSTT019rp1-2A-1 5.4 6.1 7.3 8.2 8.5 TFSTT019rp1-2A-2 5.2 6.6 8.0 8.9 9.3 TFSTT019rp1-2A-3 5.0 6.4 7.1 8.9 9.0 TFSTT019rp1-3A-1 5.5 6.5 7.4 8.2 8.9 TFSTT019rp1-3A-2 4.2 5.8 5.9 6.0 6.9 TFSTT019rp1-3A-3 5.1 6.6 7.8 8.9 9.4 TFSTT019rp1-4BA-1 4.8 6.5 8.5 7.9 9.1 TFSTT019rp1-4BA-2 5.0 6.4 6.9 8.3 9.2 TFSTT019rp1-4BA-3 AT AT AT AT AT TFSTT019rp1-4BB-1 5.2 6.6 7.5 8.1 8.8 TFSTT019rp1-4BB-2 4.7 AT 7.1 8.2 8.6 TFSTT019rp1-4BB-3 5.5 6.9 7.5 8.1 8.8 T89-01 4.7 6.0 6.5 6.9 8.3 T89-02 4.4 5.7 6.6 7.6 7.8 T89-03 4.7 6.3 6.7 7.7 8.4 T89-04 4.7 6.0 7.1 8.0 8.5 T89-05 4.0 5.3 6.3 7.8 8.1

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T89-06 4.6 5.9 6.3 7.7 8.2 T89-07 4.6 6.1 6.5 7.9 8.5 T89-08 4.0 5.2 5.8 6.9 7.1 T89-09 4.4 5.8 6.4 7.6 8.2 T89-10 4.9 6.0 6.6 7.4 8.5 T89-11 4.7 5.8 6.4 7.9 8.3 T89-12 4.7 5.9 7.3 7.4 7.6 T89-13 5.9 5.9 6.7 7.5 8.3 T89-14 4.5 5.2 5.8 6.1 7.0 T89-15 4.7 AT 6.0 6.4 7.3 T89-16 4.5 5.6 7.7 7.5 8.6 T89-17 4.1 5.4 6.0 6.8 7.5 T89-18 4.4 5.8 6.0 7.7 8.3 T89-19 4.7 5.8 7.1 8.5 9.4 T89-20 4.7 5.8 6.6 7.4 8.3 T89-21 4.7 6.2 6.3 7.7 8.5 T89-22 4.2 5.1 6.3 6.9 8.0 T89-23 4.6 5.8 7.1 7.5 8.3 T89-24 4.4 5.8 6.9 7.7 8.8 T89-25 4.5 5.4 6.2 8.0 8.7 T89-26 4.1 5.4 6.0 7.2 8.0 T89-27 4.5 5.7 6.5 7.9 9.2 T89-28 4.3 5.1 6.0 6.6 7.7 T89-29 3.5 4.7 5.5 6.3 7.1 T89-30 4.1 5.4 5.8 7.2 7.8 T89-31 5.0 5.8 6.8 7.3 8.2 T89-32 4.4 6.4 6.6 8.0 8.4

[00299] The results of the growth analysis are specified in General Table 2.66. The determined growth effects of the specified construction group are shown as ratios between AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC of the construction group and the wild type.

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Growth Rate of Maximum Diameter CO 00 O Maximum Height Growth Rate Maximum 0.93 Maximum Final Diameter 1.00 Maximum Final Height 0.95 Average Diameter Growth Rate 0.99 Maximum Height Growth Rate 0.98 Final Average Diameter 1.08 Height Average Final 0.99 Construction group Q. CÉ O) O H ιω LL H

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Construction group TFSTT036 [00300] This construction induces increased growth. The final height is 10% higher compared to the average of the construction group and the control group of the wild type. The final height is 8% higher compared to the larger individuals in the construction group and the wild type control group. The maximum height growth rate is 14% higher compared to the average for the construction group and the wild-type control group. The maximum height growth rate is 12% higher compared to the largest individuals in the construction group and the wild control group. The final diameter is 7% larger compared to the average for the construction group and the wild-type control group. The final diameter is 14% larger compared to the larger individuals in the construction group and the wild type control group. Construction group TFSTT036 meets the criteria for selecting growth differences (1) as shown in Table 2.69.

[00301] Tables 2.67 and 2.68 contain growth data for the specified construction group and the corresponding wild type group. The columns in the Tables contain measurements of height and diameter of individuals from the specified construction group and the corresponding wild type group. The measurement time as a number of days in the greenhouse is shown in the table headings. Table 2.67 Height growth data for TFSTT036

Height (cm)

Days in the greenhouse 18 25 28 32 36 39 46 53 55 TFSTT036-1B 23 37 46 56 68 79 103 126 132 TFSTT036-2A 18 36 46 57 72 83 108 126 132 TFSTT036-2B 21 33 45 54 67 80 111 135 143 TFSTT036-3A 21 36 48 59 77 90 113 133 140 TFSTT036-4A 25 39 51 61 76 88 117 141 151

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TFSTT036-4B 23 38 53 67 83 92 119 144 148 TFSTT036-5B 21 33 44 55 69 81 106 130 139 T89-01 18 32 43 54 66 76 98 124 133 T89-02 20 33 43 53 67 76 101 124 129 T89-03 17 31 43 53 68 76 102 128 135 T89-04 19 33 44 54 67 74 97 122 129 T89-05 20 33 44 54 67 76 100 121 129 T89-06 18 31 42 53 67 76 97 121 128 T89-07 16 28 41 53 65 74 96 121 129 T89-08 19 34 43 53 65 75 98 123 131 T89-09 20 30 40 52 64 73 97 119 125 T89-10 21 33 41 49 61 70 92 114 121 T89-11 18 31 40 49 61 71 94 117 123 T89-12 18 31 40 AT 60 68 90 107 115 T89-13 19 35 47 58 70 78 103 128 135 T89-14 19 31 40 50 61 72 93 118 124 T89-15 20 32 41 50 62 70 91 114 120 T89-16 18 30 39 49 62 71 92 114 122 T89-17 17 33 45 55 70 78 104 129 136 T89-18 19 32 44 56 71 82 106 130 137 T89-19 16 26 36 46 51 69 89 112 119 T89-20 16 31 41 49 60 71 91 111 118 T89-21 20 31 44 54 68 76 99 122 127 T89-22 14 26 37 46 61 72 96 120 127 T89-23 19 32 40 50 63 73 100 121 127 T89-24 20 31 41 47 58 68 90 111 117 T89-25 20 36 45 57 69 78 101 127 134 T89-26 20 37 49 58 71 80 107 131 140 T89-27 19 34 44 55 71 81 107 131 138 T89-28 17 35 44 56 68 76 99 126 132 T89-29 17 32 45 55 68 78 101 125 132 T89-30 18 31 41 50 63 72 95 119 125 T89-31 17 27 35 45 58 67 87 108 116

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T89-32 19 32 44 52 65 74 98 121 127 T89-33 20 33 43 53 64 74 96 119 126 T89-34 19 34 44 55 68 78 100 124 130 T89-35 17 30 40 51 64 74 95 116 122 T89-36 18 30 40 49 62 71 91 114 121 T89-82 21 33 43 54 65 75 98 118 125 T89-83 22 35 47 55 71 80 105 130 137 T89-85 21 36 46 56 67 76 98 123 131

Table 2.68 Diameter growth data for TFSTT036

Diameter (mm) Diameter (mm)

Days in the greenhouse 32 39 46 53 55 Days in the greenhouse 32 39 46 53 55 TFSTT036-1B 5.2 6.6 7.4 8.1 8.5 T89-17 4.8 6.7 7.0 8.3 8.4 TFSTT036-2A 5.8 6.8 8.0 9.5 8.7 T89-18 4.2 5.5 6.7 7.3 7.4 TFSTT036-2B 3.9 5.1 6.2 7.5 7.5 T89-19 4.7 5.5 6.8 7.2 7.8 TFSTT036-3A 4.2 4.2 5.1 6.2 6.4 T89-20 4.5 5.6 6.5 7.0 7.3 TFSTT036-4A 6.3 6.9 8.9 10.1 10.5 T89-21 4.9 5.6 6.4 7.4 7.6 TFSTT036-4B 5.5 6.8 8.2 8.7 8.4 T89-22 4.3 5.4 6.5 7.3 7.6 TFSTT036-5B 5.7 6.8 7.6 8.7 8.9 T89-23 3.9 5.5 6.7 7.7 7.0 T89-01 5.2 6.0 6.8 8.2 7.6 T89-24 4.3 5.9 6.8 8.1 7.9 T89-02 4.5 5.8 7.1 8.6 8.3 T89-25 4.7 6.5 7.5 9.2 9.1 T89-03 4.4 5.7 6.8 7.9 8.1 T89-26 5.4 5.9 7.7 8.6 8.8 T89-04 4.4 6.0 6.9 8.6 8.5 T89-27 4.7 5.6 7.4 7.9 8.0 T89-05 4.6 5.8 6.7 7.9 8.0 T89-28 4.7 5.7 6.3 7.2 7.4 T89-06 4.7 5.9 7.2 8.1 9.2 T89-29 4.8 5.6 6.6 7.7 8.1 T89-07 4.8 5.5 6.2 6.9 7.1 T89-30 4.6 5.5 6.7 7.3 7.3 T89-08 4.5 5.4 6.0 6.9 7.1 T89-31 4.3 5.8 6.3 7.5 7.6 T89-09 4.7 5.6 6.7 8.8 8.0 T89-32 4.6 5.9 7.6 9.3 9.1 T89-10 4.1 4.9 5.6 7.1 7.0 T89-33 4.4 5.3 6.2 7.1 7.1 T89-11 3.8 5.3 7.3 8.0 7.6 T89-34 4.6 5.6 6.8 8.1 8.7 T89-12 AT 5.1 6.2 7.3 6.9 T89-35 5.3 6.0 7.5 8.8 8.9 T89-13 4.8 5.7 6.5 7.6 7.6 T89-36 4.4 6.1 6.9 8.1 8.6 T89-14 4.2 5.5 6.6 7.6 6.5 T89-82 4.7 5.3 6.5 7.5 7.4 T89-15 5.0 6.1 7.2 7.9 8.3 T89-83 4.7 6.1 7.1 8.3 8.3 T89-16 4.4 5.6 6.2 7.3 7.9 T89-85 5.1 6.3 7.3 7.7 8.2 T89-17 4.8 6.7 7.0 8.3 8.4

[00302] The results of the growth analysis are specified in Petition 870170074044, of 29/09/2017, p. 259/378

248/362 of those in General Table 2.69. The determined growth effects of the specified construction group are shown as ratios between AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC of the construction group and the wild type.

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Growth Rate of Maximum Diameter 0.95 Maximum Height Growth Rate Maximum 1.12 Diameter Maximum End 1.14 Maximum Final Height 1.08 Average Diameter Growth Rate 0.99 Maximum Height Growth Rate 1.14 Final Average Diameter 1.07 Final Average Height 1.10 Construction group TFSTT036

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Construction Group TFSTT038 [00303] The overexpressed gene with the TFSTT038 construct generates the same top hits as the overexpressed gene with the TFSTT013 construct, when using the BLAST search against the Jamboree Gene Model database of P. tríchocarpa on the web page from the Joint Genome Institute (http://qenome.iqi-psf.org/cqi- bin / runAliqnment? db = Poptr1 1), indicating high homology between the two genes.

[00304] Tables 2.70 and 2.71 contain growth data for the specified construction group and the corresponding wild type group. The columns in the tables contain height and diameter measurements of the individuals in the specified construction group and the corresponding wild type group. The measurement time as a number of days in the greenhouse is shown in the table headings.

Table 2.70 Height growth data for TFSTT038

Days in the greenhouse 19 28 34 40 Height (cm) 44 47 51 54 61 65 TFSTT038-1A-1 23 42 62 82 92 100 110 118 138 153 TFSTT038-1A-2 19 38 58 70 83 90 102 110 130 142 TFSTT038-1B 18 33 48 62 73 83 93 99 117 128 TFSTT038-2A 21 38 58 71 85 96 108 119 143 157 TFSTT038-2B 14 29 46 60 70 78 87 96 118 130 TFSTT038-3A 18 38 57 70 83 92 105 116 133 142 TFSTT038-3B 21 38 57 72 84 92 102 110 122 126 TFSTT038-4B 17 34 52 67 78 87 96 104 123 133 T89-01 18 30 46 58 69 77 87 96 113 122 T89-02 18 30 49 62 72 77 84 90 102 109 T89-03 15 27 41 54 65 73 82 91 112 123 T89-10 AT AT AT AT AT AT AT AT AT AT T89-11 19 31 49 61 74 83 94 103 124 133 T89-12 17 30 45 58 69 77 90 100 123 134

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T89-13 18 27 43 56 68 78 91 100 121 133 T89-14 5 26 42 56 67 74 83 90 109 119 T89-15 10 15 25 33 41 45 52 57 72 82 T89-16 19 33 53 63 73 82 93 102 119 130 T89-17 17 29 46 58 66 72 80 86 96 102 T89-18 16 30 48 59 71 81 93 103 122 132 T89-19 18 32 50 65 77 84 94 102 126 139 T89-20 16 33 52 67 79 88 98 105 124 139 T89-21 17 29 44 58 67 73 83 90 110 122 T89-22 14 28 47 61 71 80 89 98 119 133 T89-23 10 33 45 53 63 71 82 90 112 123 T89-24 20 28 43 51 62 69 78 87 106 119 T89-25 14 26 38 52 64 72 82 89 110 122 T89-26 15 28 44 57 69 77 87 96 120 133 T89-27 18 29 47 62 75 82 92 103 125 138 T89-28 22 37 54 67 78 88 97 104 123 133 T89-29 16 33 49 63 76 84 93 100 123 138 T89-30 15 40 46 58 67 74 85 92 113 124

Table 2.71 Diameter growth data for TFSTT038

Days in the greenhouse 28 34 40 Diameter (mm) 44 47 51 54 61 65 TFSTT038-1A-1 4.4 6.1 7.4 8.4 9.3 9.8 10.5 11.3 11.8 TFSTT038-1A-2 3.8 5.5 6.6 7.7 7.9 8.9 9.4 10.6 11.2 TFSTT038-1B 3.4 5.1 6.5 7.2 7.8 8.3 10.1 9.3 9.5 TFSTT038-2A 3.8 5.4 6.7 7.2 8.7 9.6 9.8 11.5 11.3 TFSTT038-2B 3.8 4.4 5.4 6.4 7.2 7.8 8.5 10.1 10.7 TFSTT038-3A 3.9 5.7 7.4 7.8 8.4 9.3 9.8 10.6 11.1 TFSTT038-3B 4.3 5.9 6.8 7.8 8.0 8.0 8.4 9.0 9.9 TFSTT038-4B 4.2 4.5 6.3 6.8 7.3 8.2 8.6 9.0 9.1 T89-01 3.2 4.6 5.4 6.2 6.8 7.9 8.3 9.5 9.3 T89-02 3.4 4.7 5.5 7.3 6.3 6.6 6.9 8.3 7.5 T89-03 3.9 4.4 5.2 6.2 6.4 7.8 7.6 9.7 9.4 T89-10 AT AT AT AT AT AT AT AT AT

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T89-11 3.4 5.1 6.2 7.6 6.8 6.9 7.7 10.1 9.5 T89-12 2.9 4.7 5.8 6.6 7.8 8.0 8.7 9.1 9.2 T89-13 3.0 4.3 5.4 5.8 6.4 7.6 7.8 8.2 8.8 T89-14 3.0 4.5 5.7 6.3 6.7 7.9 7.8 9.0 8.9 T89-15 AT 2.1 3.0 4.0 4.0 4.5 4.9 5.5 5.6 T89-16 3.5 5.3 6.4 6.6 7.0 7.2 8.3 8.2 9.0 T89-17 3.4 4.6 5.1 5.4 6.0 6.4 6.5 6.8 7.1 T89-18 3.6 5.2 6.0 7.0 7.8 8.2 9.9 10.3 9.7 T89-19 4.2 5.5 6.6 7.7 8.5 8.9 9.5 11.1 12.3 T89-20 4.1 5.5 6.6 8.1 9.3 9.6 9.3 10.0 11.1 T89-21 3.1 5.6 5.8 6.7 7.1 7.8 8.4 9.7 10.1 T89-22 3.2 4.4 5.6 6.5 7.5 7.6 7.8 8.9 9.2 T89-23 2.4 4.2 5.1 6.1 6.5 7.5 10.1 9.3 10.2 T89-24 3.2 4.5 5.1 6.3 7.0 7.6 8.1 8.8 9.1 T89-25 3.3 4.3 5.2 5.8 6.5 7.4 7.8 9.3 9.7 T89-26 3.3 4.4 5.5 6.6 7.2 8.1 8.9 9.5 10.5 T89-27 3.3 4.9 6.0 7.8 8.0 8.9 9.7 11.2 11.5 T89-28 4.5 5.7 7.4 7.8 8.5 9.4 9.7 10.2 11.1 T89-29 3.1 4.7 6.3 7.2 7.9 9.2 9.8 11.1 10.7 T89-30 3.0 5.8 6.2 7.7 7.9 8.6 8.2 10.1 10.3

[00305] The results of the growth analysis are specified in General table 2.72. The determined growth effects of the specified construction group are shown as ratios between AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC of the construction group and the wild type.

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Growth Rate of Maximum Diameter 0.92 Maximum Height Growth Rate Maximum 1.05 Diameter Maximum End 0.96 Maximum Final Height 1.13 Average Diameter Growth Rate 1.06 Maximum Height Growth Rate 1.04 Final Average Diameter 1.11 Final Average Height 1.11 Construction group TFSTT038

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Construction group TFSTT045 [00306] Tables 2.73 and 2.74 contain growth data for the specified construction group and the corresponding wild type group. The columns in the tables contain height and diameter measurements of the individuals in the specified construction group and the corresponding wild type group. The measurement time as a number of days in the greenhouse is shown in the table headings. Table 2.73 Height growth data for TFSTT045

Height (cm)

Days in the greenhouse 18 25 28 32 36 39 46 53 55 TFSTT045-1B 19 33 43 52 64 75 94 116 123 TFSTT045-2B 24 37 49 58 71 80 105 127 133 TFSTT045-3A 20 35 48 60 76 87 113 143 153 TFSTT045-3B 16 28 37 47 57 65 86 111 118 TFSTT045-4A 23 34 45 56 70 82 105 133 139 TFSTT045-4B 25 38 51 60 75 87 109 119 120 TFSTT045-7B 23 35 46 60 70 80 AT 124 130 T89-01 18 32 43 54 66 76 98 124 133 T89-02 20 33 43 53 67 76 101 124 129 T89-03 17 31 43 53 68 76 102 128 135 T89-04 19 33 44 54 67 74 97 122 129 T89-05 20 33 44 54 67 76 100 121 129 T89-06 18 31 42 53 67 76 97 121 128 T89-07 16 28 41 53 65 74 96 121 129 T89-08 19 34 43 53 65 75 98 123 131 T89-09 20 30 40 52 64 73 97 119 125 T89-10 21 33 41 49 61 70 92 114 121 T89-11 18 31 40 49 61 71 94 117 123 T89-12 18 31 40 AT 60 68 90 107 115 T89-13 19 35 47 58 70 78 103 128 135 T89-14 19 31 40 50 61 72 93 118 124 T89-15 20 32 41 50 62 70 91 114 120

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T89-16 18 30 39 49 62 71 92 114 122 T89-17 17 33 45 55 70 78 104 129 136 T89-18 19 32 44 56 71 82 106 130 137 T89-19 16 26 36 46 51 69 89 112 119 T89-20 16 31 41 49 60 71 91 111 118 T89-21 20 31 44 54 68 76 99 122 127 T89-22 14 26 37 46 61 72 96 120 127 T89-23 19 32 40 50 63 73 100 121 127 T89-24 20 31 41 47 58 68 90 111 117 T89-25 20 36 45 57 69 78 101 127 134 T89-26 20 37 49 58 71 80 107 131 140 T89-27 19 34 44 55 71 81 107 131 138 T89-28 17 35 44 56 68 76 99 126 132 T89-29 17 32 45 55 68 78 101 125 132 T89-30 18 31 41 50 63 72 95 119 125 T89-31 17 27 35 45 58 67 87 108 116 T89-32 19 32 44 52 65 74 98 121 127 T89-33 20 33 43 53 64 74 96 119 126 T89-34 19 34 44 55 68 78 100 124 130 T89-35 17 30 40 51 64 74 95 116 122 T89-36 18 30 40 49 62 71 91 114 121 T89-82 21 33 43 54 65 75 98 118 125 T89-83 22 35 47 55 71 80 105 130 137 T89-85 21 36 46 56 67 76 98 123 131

Table 2.74 Diameter growth data for TFSTT045

Days in the greenhouse Diameter (mm) Days in the greenhouse Diameter (mm) 32 39 46 53 55 32 39 46 53 55 TFSTT045-1B 5.2 5.7 7.0 8.1 8.6 T89-17 4.8 6.7 7.0 8.3 8.4 TFSTT045-2B 5.5 6.7 7.8 8.8 9.1 T89-18 4.2 5.5 6.7 7.3 7.4 TFSTT045-3A 6.5 7.2 8.7 10.4 10.6 T89-19 4.7 5.5 6.8 7.2 7.8 TFSTT045-3B 4.8 5.6 7.4 8.0 8.6 T89-20 4.5 5.6 6.5 7.0 7.3 TFSTT045-4A 5.1 6.3 7.0 7.9 8.2 T89-21 4.9 5.6 6.4 7.4 7.6 TFSTT045-4B 5.5 6.1 7.3 8.0 8.8 T89-22 4.3 5.4 6.5 7.3 7.6 TFSTT045-7B 5.1 6.9 8.3 9.0 9.6 T89-23 3.9 5.5 6.7 7.7 7.0

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T89-01 5.2 6.0 6.8 8.2 7.6 T89-24 4.3 5.9 6.8 8.1 7.9 T89-02 4.5 5.8 7.1 8.6 8.3 T89-25 4.7 6.5 7.5 9.2 9.1 T89-03 4.4 5.7 6.8 7.9 8.1 T89-26 5.4 5.9 7.7 8.6 8.8 T89-04 4.4 6.0 6.9 8.6 8.5 T89-27 4.7 5.6 7.4 7.9 8.0 T89-05 4.6 5.8 6.7 7.9 8.0 T89-28 4.7 5.7 6.3 7.2 7.4 T89-06 4.7 5.9 7.2 8.1 9.2 T89-29 4.8 5.6 6.6 7.7 8.1 T89-07 4.8 5.5 6.2 6.9 7.1 T89-30 4.6 5.5 6.7 7.3 7.3 T89-08 4.5 5.4 6.0 6.9 7.1 T89-31 4.3 5.8 6.3 7.5 7.6 T89-09 4.7 5.6 6.7 8.8 8.0 T89-32 4.6 5.9 7.6 9.3 9.1 T89-10 4.1 4.9 5.6 7.1 7.0 T89-33 4.4 5.3 6.2 7.1 7.1 T89-11 3.8 5.3 7.3 8.0 7.6 T89-34 4.6 5.6 6.8 8.1 8.7 T89-12 AT 5.1 6.2 7.3 6.9 T89-35 5.3 6.0 7.5 8.8 8.9 T89-13 4.8 5.7 6.5 7.6 7.6 T89-36 4.4 6.1 6.9 8.1 8.6 T89-13 4.8 5.7 6.5 7.6 7.6 T89-82 4.7 5.3 6.5 7.5 7.4 T89-14 4.2 5.5 6.6 7.6 6.5 T89-83 4.7 6.1 7.1 8.3 8.3 T89-15 5.0 6.1 7.2 7.9 8.3 T89-85 5.1 6.3 7.3 7.7 8.2 T89-16 4.4 5.6 6.2 7.3 7.9

[00307] The results of the growth analysis are specified in General Table 2.75. The determined growth effects of the specified construction group are shown as ratios between AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC of the construction group and the wild type.

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Growth Rate of Maximum Diameter 0.94 Maximum Height Growth Rate Maximum 1.11 Maximum Final Diameter 1.16 Maximum Final Height 1.09 Average Diameter Growth Rate 1.14 Maximum Height Growth Rate 1.03 Final Average Diameter 1.15 Final Average Height 1.03 Construction group TFSTT045

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Construction groups TFSTT051Rp1 [00308] Tables 2.76 and 2.77 contain growth data for the specified construction group and the corresponding wild type group. The columns in the tables contain height and diameter measurements of the individuals in the specified construction group and the corresponding wild type group. The measurement time as a number of days in the greenhouse is shown in the table headings. Table 2.76 Height growth data for TFSTT051 Rp1

Height (cm)

Days in the greenhouse 20 25 29 35 41 46 50 53 57 60 TFSTT051rp1-3B-1 19 25 29 44 60 70 76 83 90 97 TFSTT051rp1-3B-2 17 24 30 43 56 68 75 82 89 97 TFSTT051rp1-3B-3 18 24 28 37 49 63 69 75 83 90 TFSTT051rp1-4A-1 18 23 30 43 58 71 81 90 101 110 TFSTT051rp1-4A-2 16 25 33 45 62 78 88 97 109 119 TFSTT051rp1-4A-3 20 28 38 54 73 90 101 110 122 132 T89-01 17 26 31 45 61 76 87 95 107 116 T89-02 16 21 26 38 53 67 76 83 AT AT T89-03 18 24 32 47 64 78 87 96 106 115 T89-04 18 26 35 50 66 80 87 96 107 115 T89-05 17 26 33 44 57 73 81 91 100 107 T89-06 16 21 28 40 55 74 79 89 99 106 T89-07 17 23 31 43 57 71 80 88 98 107 T89-08 15 20 26 37 51 65 75 84 94 100 T89-09 18 25 32 46 61 74 85 93 103 112 T89-10 19 25 32 46 60 75 86 95 104 112 T89-11 20 27 36 51 68 86 98 107 120 127 T89-12 19 27 36 50 67 80 89 97 108 118 T89-13 18 25 32 45 59 75 83 92 101 108 T89-14 18 23 26 36 51 65 72 79 89 96 T89-15 17 21 28 39 53 70 78 87 97 105 T89-16 19 25 32 43 57 71 81 90 101 109 T89-17 16 20 29 41 54 68 78 84 93 101 T89-18 16 23 30 45 63 78 87 94 103 110 T89-19 16 22 28 42 56 69 79 89 100 107 T89-20 18 25 32 47 63 78 89 99 109 115 T89-21 19 27 34 50 67 82 92 102 111 120 T89-22 19 25 32 44 58 76 84 93 102 109 T89-23 18 26 33 47 63 79 88 99 108 116 T89-24 16 24 28 42 53 70 78 85 94 103 T89-25 16 25 32 45 61 76 85 95 107 114

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T89-26 16 20 26 38 52 68 78 86 97 103 T89-27 16 21 25 35 48 61 70 78 AT 98 T89-28 14 18 25 38 51 64 73 81 90 96 T89-29 7 13 18 30 45 60 69 77 90 98 T89-30 15 22 29 42 55 69 80 88 97 106 T89-31 21 29 37 50 65 80 88 98 108 116 T89-32 19 24 32 42 55 70 78 87 96 105

Table 2.77 Diameter growth data for TFSTT051Rp1

Days in the greenhouse 35 Diameter (mm) 41 46 53 60 Days in the greenhouse 35 Diameter (mm ' 41 46 53 60 TFSTT051rp1-3B-1 4.6 5.9 6.4 7.2 7.7 T89-14 4.5 5.2 5.8 6.1 7.0 TFSTT051rp1-3B-2 4.3 5.6 6.0 6.1 7.3 T89-15 4.7 AT 6.0 6.4 7.3 TFSTT051rp1-3B-3 4.2 5.4 6.8 6.9 8.3 T89-16 4.5 5.6 7.7 7.5 8.6 TFSTT051rp1-4A-1 4.5 5.9 6.6 7.8 9.1 T89-17 4.1 5.4 6.0 6.8 7.5 TFSTT051rp1-4A-2 4.5 5.9 6.6 7.4 8.1 T89-18 4.4 5.8 6.0 7.7 8.3 TFSTT051rp1-4A-3 5.2 6.4 7.1 7.9 8.3 T89-19 4.7 5.8 7.1 8.5 9.4 T89-01 4.7 6.0 6.5 6.9 8.3 T89-20 4.7 5.8 6.6 7.4 8.3 T89-02 4.4 5.7 6.6 7.6 7.8 T89-21 4.7 6.2 6.3 7.7 8.5 T89-03 4.7 6.3 6.7 7.7 8.4 T89-22 4.2 5.1 6.3 6.9 8.0 T89-04 4.7 6.0 7.1 8.0 8.5 T89-23 4.6 5.8 7.1 7.5 8.3 T89-05 4.0 5.3 6.3 7.8 8.1 T89-24 4.4 5.8 6.9 7.7 8.8 T89-06 4.6 5.9 6.3 7.7 8.2 T89-25 4.5 5.4 6.2 8.0 8.7 T89-07 4.6 6.1 6.5 7.9 8.5 T89-26 4.1 5.4 6.0 7.2 8.0 T89-08 4.0 5.2 5.8 6.9 7.1 T89-27 4.5 5.7 6.5 7.9 9.2 T89-09 4.4 5.8 6.4 7.6 8.2 T89-28 4.3 5.1 6.0 6.6 7.7 T89-10 4.9 6.0 6.6 7.4 8.5 T89-29 3.5 4.7 5.5 6.3 7.1 T89-11 4.7 5.8 6.4 7.9 8.3 T89-30 4.1 5.4 5.8 7.2 7.8 T89-12 4.7 5.9 7.3 7.4 7.6 T89-31 5.0 5.8 6.8 7.3 8.2 T89-13 5.9 5.9 6.7 7.5 8.3 T89-32 4.4 6.4 6.6 8.0 8.4

[00309] The results of the growth analysis are specified in General Table 2.78. The determined growth effects of the specified construction group are shown as ratios between AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC of the construction group and the wild type.

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Growth Rate of Maximum Diameter 0.95 Maximum Height Growth Rate Maximum 0.98 Maximum Final Diameter 0.97 Maximum Final Height 1.04 Average Diameter Growth Rate 0.95 Maximum Height Growth Rate 0.96 Final Average Diameter 1.00 Height Average Final 0.99 Construction group Q. HEAVEN m o I— ιω LL H

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Example 3

Calculation of volumetric growth [00310] The trunk volume of each individual plant is approximated from the final height and final diameter measurements using a cone volume.

[00311] Approximation of trunk volume _ π * Κ * τ ² ~ 3 [00312] where [00313] V = Volume [00314] h = height (Final height) [00315] r = radius (Final diameter / 2) [00316] The average final volumes of each construction group population and the corresponding wild type population are calculated subsequently. The volumetric growth selection criterion is applied, in which a construction group is considered to have a significant or pronounced increase in volume compared to the wild type population if the average final volume of the construction group is at least 25% (or 50% at the second most stringent level) greater than the average final volume of the corresponding wild type group.

[00317] The results from the volume approximation are specified in Table 3.1. The determined growth effects are presented as ratios between the mean final volumes (LFA) of the wild type group.

[00318] The following construction groups satisfy the criterion of volumetric growth. The TF0002Rp2 construction group has an average final volume increase of 36%; the TF0013 group has an average final volume increase of 27%; construction group TF0045 has an average final volume increase of 33%; the group of

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262/362 construction TF0096 has an average final volume increase of 44%; construction group TF0109 has an average final volume increase of 44%; construction group TF0116 has an average final volume increase of 31%; the TF0132rp1 construction group has an average final volume increase of 46%, whereas the TF0132rp1-4AC construction group strain has an average final volume increase of 70% (± 20%); construction group TF0146 has an average final volume increase of 34%; the construction group TF0247 has an average final volume increase of 49%; construction group TF045 has an average final volume increase of 45%; the construction group TFSTT016 has an average final volume increase of 36%; the construction group TFSTT036 has an average final volume increase of 28%; the construction group TFSTT038 has an average final volume increase of 32%; the construction group TFSTT045 has an average final volume increase of 38%.

[00319] The following groups satisfy the most stringent level of the volumetric growth criterion (5) as shown in Table 3.1. Construction group TF0097Rp1 has an increase in average final volume of 68%, whereas the lineage of construction group TF0097Rp1-3A has an increase in average final volume of 116% (± 37%); construction group TF0104 has an average final volume increase of 79%; the construction group TF109Rp1 has an increase in average final volume of 58%, whereas the lineage of construction group TF019Rp1-4A has an increase in average final volume of 92% (± 5%); the construction group TF0132.2nd has an average final volume increase of 65%; the construction group TFSTT004 has an average final volume increase of 51%. These construction groups satisfy the most stringent level of the volumetric growth criterion (5) as shown in Table 3.1.

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Table 3.1

Construction group Average Final Volume TF0002Rp2 1.36 TF0013 1.27 TF0045 1.33 TF0096 1.44 TF0097Rp1 1.68 TF0104 1.79 TF0109 1.44 TF0109Rp1 1.58 TF0116 1.31 TF0132.2nd 1.65

Example 4

Dry weight measurements

Construction group Average final volume TF0132rp1 1.46 TF0146 1.34 TF0247 1.49 TF0405 1.45 TFSTT004 1.51 TFSTT016 1.36 TFSTT036 1.28 TFSTT038 1.32 TFSTT045 1.38

[00320] Dry weight measurements were performed in replanted construction groups. The plants were harvested according to standard procedure: trunk, bark, five fully developed leaves, leaf remnants and roots were collected as separate samples. The leaf areas were measured from the five fully developed leaves and the length of the 20 fully developed internodes was measured. The plant material samples were placed in a dryer oven for more than 48 hours. Dry weights were measured and analyzed according to the differences compared with the corresponding wild type groups. Abbreviations and parameters used for dry weight analysis are shown in Table 4.1.

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Table 4.1 Abbreviations and parameters associated with dry weight

Trunk (g) Dry weight of the trunk of an individual plant Shell (g) Dry bark weight of an individual plant Root (g) Dry root weight of an individual plant Sheet (g) Dry weight of all leaves of an individual plant TOTAL (g) Total dry weight of the trunk, bark and leaves of an individual plant SLA (m ² / kg) Specific leaf area, square meter per kg dry leaf weight, calculated from five fully developed leaves of an individual plant Internode (cm) Average internode length, calculated from 20 internodes of an individual plant Medium Trunk Average dry weight of the trunk of the wild type population and each population of the construction group Medium Bark Average dry bark weight of the wild type population and each population of the construction group Mean root Average dry weight of the root of the wild type population and each population of the construction group Medium Sheet Average dry leaf weight and each population of the construction group TOTAL average Dry weight Average total wild-type population and each population in the construction group Average SLA Average SLA of the wild type population and each population of the construction group Medium internode Average internode of the wild type population and each population of the group of each construction Maximum trunk Maximum dry weight of the trunk of the wild type population and each population of the construction group Maximum Bark Maximum dry shell weight of the wild type population and each population of the construction group Maximum Root Average dry weight of the root of the wild type population and each strain of the construction group Maximum Sheet Maximum dry leaf weight of the wild type population and each population of the construction group TOTAL Maximum Dry weight Total maximum of the six-type population

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and each population in the construction group Medium Lineage Trunk Average dry weight of the trunk of the wild type population and each strain of the construction group Average Lineage Bark Average dry bark weight of the wild type population and each strain of the construction group Average Lineage Root Average dry weight of the root of the wild type population and each strain of the construction group Average Lineage Leaf Average dry leaf weight of the wild type population and each strain of the construction group TOTAL average of the lineage Average total dry weight of the wild type population and each strain of the construction group Lineage Average SLA Average SLA of the wild type population and each strain of the construction group Medium Lineage Internode Average internode length of the wild type population and each strain of the construction group

Results of dry weight experiments

Construction group TF0013 [00321] This construction induces an increase in biomass production. Dry weight measurements from the construction group show a 20% increase in the average trunk, 14% increase in the average bark, 14% increase in the average leaf and 16% increase in the average TOTAL compared to the corresponding wild type group. One of the strains in the construction group shows a 48% increase in the average trunk, 37% increase in the average bark, 31% increase in the average leaf and 36% of the average TOTAL compared to the corresponding wild type group.

[00322] Table 4.2 contains dry weight data for the specified construction group and corresponding wild type group

Table 4.2

Individual Construction Group Trunk (g) Bark (g) Root (g) leaf (g) TOTAL (g) SLA (m ² / kg) Internode (cm) TF0013rp2-1A-1 4.4 2.1 15.1 21.6 33.7 3.0 TF0013rp2-1A-2 6.5 2.7 15.0 24.2 41.2 3.6 TF0013rp2-1A-3 5.9 2.8 14.4 23.0 33.6 3.5

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Individual Construction Group Trunk (g) Bark (g) Root (g) leaf (g) TOTAL (g) SLA (m ² / kg) Internode (cm) TF0013rp2-3BA-1 8.7 3.7 19.5 31.9 34.4 3.8 TF0013rp2-3BA-2 9.1 3.9 20.6 33.6 32.8 3.7 TF0013rp2-3BA-3 8.7 3.7 19.8 32.2 34.2 4.1 T89-20 5.8 2.7 6.4 14.0 22.5 35.4 3.7 T89-21 5.9 3.0 8.5 16.9 25.8 30.3 3.2 T89-22 6.0 2.7 15.4 24.1 34.4 3.7 T89-23 4.3 2.0 4.6 12.1 18.4 48.9 3.3 T89-24 5.5 2.5 6.5 14.3 22.2 32.8 3.6 T89-25 7.6 3.4 8.0 18.8 29.8 31.2 3.3 T89-26 6.6 3.0 17.2 26.8 33.5 3.7 T89-27 6.9 3.1 16.0 26.0 31.4 3.8 T89-28 5.2 2.6 12.8 20.5 32.7 3.4

[00323] Table 4.3 contains the dry weight ratios of the specified construction group relative to the corresponding wild type group of the medium trunk, medium bark, medium root, medium leaf and average TOTAL. Table 4.3 also shows average SLA ratios and average internode length for the specified construction group relative to the corresponding wild type group.

Table 4.3

Group of Construction Medium Trunk Medium Bark Average Root Medium Sheet TOTAL Medium Average SLA Medium internode TF0013r p2 1.20 1.14 1.14 1.16 1.01 1.03

[00324] Tabe a 4.4 contains the dry weight ratios for the specified construction group relative to the corresponding wild type group for maximum trunk, maximum bark, maximum root, maximum leaf and maximum TOTAL

Table 4.4

Group of Trunk Bark Root leaf TOTAL

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Construction Maximum Maximum Maximum Maximum maximum TF0013rp2 1.20 1.16 1.09 1.13

[00325] Table 4.5 contains dry weight ratios for the specified building group strain relative to the corresponding wild type group of the medium trunk, medium bark, medium root, medium leaf and average TOTAL. Table 4.5 also shows average SLA ratios and average internode length of the lineage of the specified construction group relative to the corresponding wild type group.

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Medium Lineage Internode 0.96 1.10 Lineage Average SLA 1.05 0.98 TOTAL Average Lineage 0.95 1.36 Average Lineage Leaf 0.97 1.31 Average Lineage Root Average Lineage Bark 0.91 1.37 Medium Lineage Trunk 0.93 co Lineage of Group of construction TF0013rp2-1A TF0013rp2-3BA

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Construction Group TF0132 [00326] This construction induces an increase in biomass production. Dry weight measurements from the construction group show 83% increase in the average trunk, 58% increase in the average bark, 34% increase in the average leaf and 49% increase in the average TOTAL compared to the corresponding wild type group. One of the strains in the construction group shows 119% increase in the average trunk, 82% increase in the average bark, 53% increase in the average leaf and 73% of the average TOTAL compared to the corresponding wild type group. For strains in which the dry root weights were measured, an increase in the sprout-root ratio was observed.

[00327] Table 4.6 contains dry weight data for the specified construction group and the corresponding wild type group.

Table 4.6

Individual Construction Group Trunk (g) Bark (g) Root (g) leaf (g) TOTAL (g) SLA (m ² / kg) Internode (cm) TF0132rp1-1B-2 11.7 4.6 10.3 18.1 34.5 33.3 TF0132rp1-1B-3 9.1 3.5 6.0 15.7 28.4 33.3 4.2 TF0132rp1-3BB-1 9.8 4.1 10.7 18.7 32.6 33.7 4.2 TF0132rp1-3BB-2 11.4 4.9 9.8 24.8 41.2 30.8 4.3 TF0132rp1-3BB-3 10.8 4.3 10.7 20.4 35.5 34.1 4.3 TF0132rp1-4AC-1 13.3 5.1 20.7 39.1 32.8 3.6 TF0132rp1-4AC-2 14.5 5.5 26.7 46.7 34.2 3.9 TF0132rp1-4AC-3 11.4 4.5 22.5 38.4 34.6 3.7 TF0132rp1-4B-1 10.7 4.3 18.2 33.2 32.4 4.1 TF0132rp1-4B-2 6.7 2.9 16.9 26.4 34.3 4.0 TF0132rp1-4B-3 10.2 3.8 17.7 31.7 38.9 4.1 TF0132rp1-6B-1 7.5 3.6 21.3 32.5 32.5 4.0 TF0132rp1-6B-2 11.9 4.6 20.7 37.2 34.0 4.3 TF0132rp1-6B-3 11.1 4.6 22.5 38.2 31.4 4.3 T89-20 5.8 2.7 6.4 14.0 22.5 35.4 3.7 T89-21 5.9 3.0 8.5 16.9 25.8 30.3 3.2 T89-22 6.0 2.7 15.4 24.1 34.4 3.7

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T89-23 4.3 2.0 4.6 12.1 18.4 48.9 3.3 T89-24 5.5 2.5 6.5 14.3 22.2 32.8 3.6 T89-25 7.6 3.4 8.0 18.8 29.8 31.2 3.3 T89-26 6.6 3.0 17.2 26.8 33.5 3.7 T89-27 6.9 3.1 16.0 26.0 31.4 3.8 T89-28 5.2 2.6 12.8 20.5 32.7 3.4

[00328] Table 4.7 contains the dry weight ratios of the specified construction group relative to the corresponding wild type group of medium trunk, medium bark, medium root, medium leaf and average TOTAL. Table 4.7 also shows average SLA ratios and average internode length for the specified construction group relative to the corresponding wild type group.

Table 4.7

Construction Group Medium Trunk Medium Bark Average Root leaf Average TOTAL Average Average SLA Medium internode TF0132rp1 1.83 1.58 1.58 1.34 1.49 0.96 1.17

[00329] Table 4.8 contains the dry weight ratios of the specified construction group relative to the corresponding wild type group for maximum trunk, maximum bark, maximum root, maximum leaf and maximum TOTAL.

Table 4.8

Construction Group Maximum trunk Maximum Bark Maximum Root Maximum Sheet TOTAL maximum TF0132rp 1 1.92 1.62 1.99 1.42 1.57

[00330] Table 4.9 contains the dry weight ratios for the specified construction group relative to the corresponding wild type group of medium trunk, medium bark, medium root, medium leaf and average TOTAL. Table 4.9 also shows average SLA ratios and average internode length of the lineage of the specified construction group with respect to the corresponding wild type group.

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Medium Lineage Internode 1.25 1.22 1.07 1.15 1.20 Lineage Average SLA 0.92 0.95 0.98 1.02 0.95 TOTAL Average Lineage m 1.52 1.73 1.27 1.50 Average Lineage Leaf 1.24 1.40 1.53 1.15 1.41 Average Lineage Root 1.63 1.53 Average Lineage Bark 1.61 1.59 1.82 1.32 1.53 Medium Lineage Trunk 1.93 1.79 2.19 1.54 1.70 Lineage of Construction Group TF0132rp1-1B TF0132rp1-3BB TF0132rp1-4AC TF0132rp1-4B TF0132rp1-6B

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Construction Group TF0002 [00331] This construction induces an increase in biomass production. Dry weight measurements from the construction group show 50% increase in the average trunk, 52% increase in the average bark, 6% increase in the average leaf and 20% increase in the average TOTAL compared to the corresponding wild type group. One of the strains in the construction group shows a 72% increase in the average trunk, 61% increase in the average bark, 20% increase in the average leaf and 35% increase in the average TOTAL compared to the corresponding wild type group. For the strain where the dry root weight was measured, an increase in the sprout-root ratio was observed.

[00332] Table 4.10 contains dry weight data for the specified construction group and the corresponding wild type group.

Table 4.10

Individual Construction Group Trunk (g) Bark (g) Root (g) leaf (g) TOTAL (g) SLA (m ² / kg) Internode (cm) TF0002rp2-1B-1 8.5 3.4 8.1 17.4 29.4 33.4 3.5 TF0002rp2-1B-2 10.7 4.7 12.6 20.5 35.8 32.2 3.2 TF0002rp2-1B-3 10.0 3.7 8.0 19.2 32.9 36.2 3.3 TF0002rp2-2A-1 8.0 3.4 14.5 25.9 34.3 3.3 TF0002rp2-2A-2 5.0 2.4 10.1 17.4 35.9 3.0 TF0002rp2-2A-3 6.4 3.3 12.2 22.0 25.3 3.1 TF0002rp2-3B-1 4.7 2.3 12.0 19.0 34.5 3.0 TF0002rp2-3B-2 10.7 4.7 20.7 36.1 32.1 3.3 TF0002rp2-3B-3 12.3 5.8 25.3 43.3 27.4 2.5 T89-01 5.1 2.2 13.1 20.4 39.9 2.7 T89-02 13.8 28.4 36.3 2.6 T89-03 6.8 2.7 17.1 26.6 37.8 2.6 T89-04 6.9 2.7 17.3 27.0 37.1 2.4 T89-05 5.7 2.5 16.6 24.9 31.8 2.6 T89-06 6.1 2.7 8.2 18.4 27.1 31.7 2.7 T89-07 5.8 2.5 9.2 15.9 24.2 31.7 2.6 T89-08 3.9 2.1 16.1 22.0 29.2 2.6

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T89-09 6.9 3.1 20.7 30.7 30.3 2.5 T89-10 5.5 2.5 10.3 16.6 24.6 36.9 2.6 T89-11 6.9 3.0 17.9 27.8 34.0 3.0 T89-12 6.6 3.0 15.2 24.8 31.9 2.8 T89-13 5.9 2.2 15.0 23.1 39.6 2.4 T89-14 3.4 1.7 13.2 18.3 32.7 2.5 T89-15 4.6 2.0 14.9 21.6 33.4 2.7 T89-16 6.1 2.7 17.7 26.4 31.8 2.6 T89-17 4.4 2.1 15.9 22.4 31.5 2.6 T89-18 5.6 2.6 15.0 23.3 32.7 2.7 T89-19 6.2 2.7 18.4 27.3 33.6 2.5 T89-20 5.9 2.7 8.4 16.5 25.2 35.9 2.7 T89-21 6.9 2.8 17.8 27.5 39.0 2.6 T89-22 5.4 2.2 13.7 21.3 37.9 2.7 T89-23 7.0 2.9 17.7 27.5 33.3 2.9 T89-24 6.6 2.7 17.3 26.6 33.8 2.6 T89-25 6.5 2.8 7.4 17.2 26.5 32.4 2.8 T89-26 4.7 2.2 6.2 14.3 21.2 33.0 2.8 T89-27 5.4 2.4 16.1 23.9 31.3 2.5 T89-28 3.6 1.5 11.7 16.7 39.5 2.5 T89-29 3.1 1.3 9.9 14.4 36.3 3.0 T89-30 5.0 2.1 13.7 20.8 36.2 2.9 T89-31 7.0 2.9 17.2 27.2 32.7 2.7 T89-32 6.0 2.5 16.7 25.2 32.3 2.5

[00333] Table 4.11 contains the dry weight ratios of the specified construction group relative to the corresponding wild type group of medium trunk, medium bark, medium root, medium leaf and average TOTAL. Table 4.11 also shows average SLA ratios and average internode length for the specified construction group relative to the corresponding wild type group.

Table 4.11

Construction Group Medium Trunk Medium Bark Average Root Medium Sheet TOTAL Average Average SLA Medium internode TF0002rp2 1.50 1.52 1.16 1.06 1.20 0.94 1.19

[00334] Table 4.12 contains the dry weight ratios for the group

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274/362 of construction specified for the corresponding wild type group of maximum trunk, maximum bark, maximum root, maximum leaf and maximum TOTAL.

Table 4.12

Construction Group Maximum trunk Maximum Bark Maximum Root Maximum Sheet TOTAL maximum TF0002rp2 1.76 1.85 1.23 1.22 1.41

[00335] Table 4.13 contains the dry weight ratios for the lineage of the specified construction group relative to the corresponding wild type group of medium trunk, medium bark, medium root, medium leaf and average TOTAL. Table 4.13 also shows average SLA ratios and average internode length for the specified construction group relative to the corresponding wild type group.

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Medium Lineage Internode 1.27 00 1.12 Lineage Average SLA 0.99 0.93 0.91 Average TOTAL Lineage 1.35 0.90 1.36 Average Lineage Leaf 1.20 O 1.22 Average Lineage Root 1.16 Average Lineage Bark 1.61 1.23 1.73 Medium Lineage Trunk 1.72 1.15 1.63 Lineage of Group of Construction TF0002rp2-1B TF0002rp2-2A TF0002rp2-3B

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Construction Group TF0052 [00336] This construction induces an increase in biomass production. Dry weight measurements of one of the strains in the construction group show a 49% increase in the average trunk, 64% increase in the average bark, 32% increase in the average leaf and 38% increase in the average TOTAL compared to the group in the corresponding wild type.

[00337] Table 4.14 contains dry weight data for the specified construction group and the corresponding wild type group.

Table 4.14

Construction Group Individuals Trunk (9) Shell (g) Root (g) leaf (g) TOTAL (g) SLA (m ² / kg) Internode (cm) TF0052rp1-2A-1 9.5 4.5 23.8 37.8 30.0 3.0 TF0052rp1-2A-2 7.3 3.6 18.4 29.2 33.7 3.0 TF0052rp1-2A-3 8.5 4.0 21.0 33.4 37.8 3.0 TF0052rp1-2B-1 4.7 2.2 14.1 21.0 39.8 2.6 TF0052rp1-2B-2 4.0 2.0 13.5 19.6 32.3 2.6 TF0052rp1-2B-3 2.4 1.3 7.3 11.0 31.0 2.5 T89-01 5.1 2.2 13.1 20.4 39.9 2.7 T89-02 13.8 28.4 36.3 2.6 T89-03 6.8 2.7 17.1 26.6 37.8 2.6 T89-04 6.9 2.7 17.3 27.0 37.1 2.4 T89-05 5.7 2.5 16.6 24.9 31.8 2.6 T89-06 6.1 2.7 8.2 18.4 27.1 31.7 2.7 T89-07 5.8 2.5 9.2 15.9 24.2 31.7 2.6 T89-08 3.9 2.1 16.1 22.0 29.2 2.6 T89-09 6.9 3.1 20.7 30.7 30.3 2.5 T89-10 5.5 2.5 10.3 16.6 24.6 36.9 2.6 T89-11 6.9 3.0 17.9 27.8 34.0 3.0 T89-12 6.6 3.0 15.2 24.8 31.9 2.8 T89-13 5.9 2.2 15.0 23.1 39.6 2.4

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T89-14 3.4 1.7 13.2 18.3 32.7 2.5 T89-15 4.6 2.0 14.9 21.6 33.4 2.7 T89-16 6.1 2.7 17.7 26.4 31.8 2.6 T89-17 4.4 2.1 15.9 22.4 31.5 2.6 T89-18 5.6 2.6 15.0 23.3 32.7 2.7 T89-19 6.2 2.7 18.4 27.3 33.6 2.5 T89-20 5.9 2.7 8.4 16.5 25.2 35.9 2.7 T89-21 6.9 2.8 17.8 27.5 39.0 2.6 T89-22 5.4 2.2 13.7 21.3 37.9 2.7 T89-23 7.0 2.9 17.7 27.5 33.3 2.9 T89-24 6.6 2.7 17.3 26.6 33.8 2.6 T89-25 6.5 2.8 7.4 17.2 26.5 32.4 2.8 T89-26 4.7 2.2 6.2 14.3 21.2 33.0 2.8 T89-27 5.4 2.4 16.1 23.9 31.3 2.5 T89-28 3.6 1.5 11.7 16.7 39.5 2.5 T89-29 3.1 1.3 9.9 14.4 36.3 3.0 T89-30 5.0 2.1 13.7 20.8 36.2 2.9 T89-31 7.0 2.9 17.2 27.2 32.7 2.7 T89-32 6.0 2.5 16.7 25.2 32.3 2.5

[00338] Tabe a 4.15 contains the dry weight ratios for the specified construction group relative to the corresponding wild type group of medium trunk, medium bark, medium root, medium leaf and average TOTAL. Table 4.15 also shows average SLA ratios and average internode length for the specified construction group relative to the corresponding wild type group.

Table 4.15

Construction Group Medium Trunk Medium Bark Average Root leaf Average TOTAL Medium Average SLA Medium internode TF0052rp1 1.07 1.20 1.03 1.05 0.99 1.05 [00339] Table 4 k16 con have dry weight ratios for the group

specified construction related to the wild type group correspondsPetition 870170074044, of 29/09/2017, p. 289/378

278/362 maximum trunk weight, maximum bark, maximum root, maximum leaf and maximum TOTAL.

Table 4.16

Construction Group Maximum trunk Maximum Bark Maximum Root Maximum Sheet TOTAL Maximum TF0052rp1 1.36 1.43 1.15 1.23

[00340] Table 4.17 contains the dry weight ratios for the lineage of the specified construction group relative to the corresponding wild type group of medium trunk, medium bark, medium root, medium leaf and average TOTAL. Table 4.17 also shows average SLA ratios and average internode length for the specified construction group relative to the corresponding wild type group.

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Medium Lineage Internode 1.12 0.97 Lineage Average SLA 0.99 1.00 TOTAL Line Average- gem 1.38 0.71 Average Lineage Leaf 1.32 0.73 Average Lineage Root Average Lineage Bark 1.64 0.76 Medium Lineage Trunk 1.49 0.65 Lineage of Group of construction TF0052rp1-2A TF0052rp1-2B

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Construction Group TF0076 [00341] This construction induces an increase in biomass production. Dry weight measurements from the construction group show a 16% increase in the average trunk, 11% increase in the average bark, 4% increase in the average leaf and 7% increase in the average TOTAL compared to the corresponding wild type group. One of the strains in the construction group shows a 42% increase in the average trunk, 29% increase in the average bark, 16% increase in the average leaf and 23% increase in the average TOTAL compared to the corresponding wild type group. For the strain where the dry root weight was measured, an increase in the sprout-root ratio was observed.

[00342] Table 4.18 contains dry weight data for the specified construction group and the corresponding wild type group.

Table 4.18

Construction Group Individual Trunk (9) Shell (g) Root (g) leaf (g) TOTAL (g) SLA (m ² / kg) Internode (cm) TF0076rp2-3BB-1 5.8 2.7 15.7 24.2 32.9 3.0 TF0076rp2-3BB-2 6.9 3.1 17.0 27.1 29.3 2.7 TF0076rp2-3BB-3 8.0 3.5 23.1 34.6 33.0 2.8 TF0076rp2-4B-1 7.9 3.2 7.8 19.6 30.6 33.1 2.6 TF0076rp2-4B-2 7.5 3.2 7.7 18.0 28.7 35.4 2.7 TF0076rp2-4B-3 8.7 3.1 8.1 17.9 29.6 37.3 2.3 TF0076rp2-5BA-1 3.9 1.5 9.0 14.4 43.8 2.3 TF0076rp2-5BA-2 6.6 2.7 14.7 23.9 36.8 2.8 TF0076rp2-5BA-3 7.6 2.8 18.1 28.5 36.2 2.9 TF0076rp2-5BB-1 5.4 2.2 14.9 22.4 39.2 3.0 TF0076rp2-5BB-2 5.5 2.6 17.2 25.3 32.6 2.9 TF0076rp2-5BB-3 5.4 2.1 12.8 20.2 28.5 2.5 T89-01 5.1 2.2 13.1 20.4 39.9 2.7 T89-02 13.8 28.4 36.3 2.6

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T89-03 6.8 2.7 17.1 26.6 37.8 2.6 T89-04 6.9 2.7 17.3 27.0 37.1 2.4 T89-05 5.7 2.5 16.6 24.9 31.8 2.6 T89-06 6.1 2.7 8.2 18.4 27.1 31.7 2.7 T89-07 5.8 2.5 9.2 15.9 24.2 31.7 2.6 T89-08 3.9 2.1 16.1 22.0 29.2 2.6 T89-09 6.9 3.1 20.7 30.7 30.3 2.5 T89-10 5.5 2.5 10.3 16.6 24.6 36.9 2.6 T89-11 6.9 3.0 17.9 27.8 34.0 3.0 T89-12 6.6 3.0 15.2 24.8 31.9 2.8 T89-13 5.9 2.2 15.0 23.1 39.6 2.4 T89-14 3.4 1.7 13.2 18.3 32.7 2.5 T89-15 4.6 2.0 14.9 21.6 33.4 2.7 T89-16 6.1 2.7 17.7 26.4 31.8 2.6 T89-17 4.4 2.1 15.9 22.4 31.5 2.6 T89-18 5.6 2.6 15.0 23.3 32.7 2.7 T89-19 6.2 2.7 18.4 27.3 33.6 2.5 T89-20 5.9 2.7 8.4 16.5 25.2 35.9 2.7 T89-21 6.9 2.8 17.8 27.5 39.0 2.6 T89-22 5.4 2.2 13.7 21.3 37.9 2.7 T89-23 7.0 2.9 17.7 27.5 33.3 2.9 T89-24 6.6 2.7 17.3 26.6 33.8 2.6 T89-25 6.5 2.8 7.4 17.2 26.5 32.4 2.8 T89-26 4.7 2.2 6.2 14.3 21.2 33.0 2.8 T89-27 5.4 2.4 16.1 23.9 31.3 2.5 T89-28 3.6 1.5 11.7 16.7 39.5 2.5 T89-29 3.1 1.3 9.9 14.4 36.3 3.0 T89-30 5.0 2.1 13.7 20.8 36.2 2.9 T89-31 7.0 2.9 17.2 27.2 32.7 2.7 T89-32 6.0 2.5 16.7 25.2 32.3 2.5

[00343] Table 4.19 contains the dry weight ratios of the specified construction group with respect to the corresponding wild type group of medium trunk, medium bark, medium root, medium leaf and

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TOTAL average. Table 4.19 also shows average SLA ratios and average internode length for the specified construction group relative to the corresponding wild type group.

Table 4.19

Construction Group Medium Trunk Medium Bark Average Root leaf Average TOTAL Medium Average SLA Medium internode TF0076 rp2 1.16 1.11 0.95 1.04 1.07 1.02 1.02 [00344] Table 4 L20 con have dry weight ratios for the group

specified construction type for the corresponding wild type group of maximum trunk, maximum bark, maximum root, maximum leaf and maximum TOTAL.

Table 4.20

Construction Group Maximum trunk Maximum Bark Maximum Root Maximum Sheet TOTAL Maximum TF0076rp2 1.24 1.13 0.79 1.12 1.13

[00345] Table 4.21 contains the dry weight ratios for the lineage of the specified construction group with respect to the corresponding wild type group of medium trunk, medium bark, medium root, medium leaf and average TOTAL. Table 4.21 also shows average SLA ratios and average internode length for the specified construction group relative to the corresponding wild type group.

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Medium Lineage Internode 1.06 0.96 1.00 1.06 Lineage Average SLA 0.92 1.03 1.14 0.98 TOTAL Average Lineage 00 1.23 0.92 0.94 Average Lineage Leaf 1.17 1.16 00 00 o 0.94 Average Lineage Root 0.95 Average Lineage Bark 1.27 1.29 0.94 0.94 Medium Lineage Trunk 1.22 1.42 1.06 0.95 Lineage of Group of construction TF0076rp2-3BB TF0076rp2-4B TF0076rp2-5BA TF0076rp2-5BB

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Construction Group TF0097 [00346] This construction induces an increase in biomass production. Dry weight measurements from the construction group show 74% increase in the average trunk, 82% increase in the average bark, 28% increase in the average leaf and 43% increase in the average TOTAL compared to the corresponding wild type group. One of the strains in the construction group shows 136% increase in the average trunk, 141% increase in the average bark, 63% increase in the average leaf and 87% increase in the average TOTAL compared to the corresponding wild type group. For the strain where the dry root weight was measured, an increase in the sprout-root ratio was observed.

[00347] Table 4.22 contains dry weight data for the specified construction group and the corresponding wild type group.

Table 4.22

Construction Group Individual Trunk (9) Bark (g) Root (g) leaf (g) TOTAL (g) SLA (m ² / kg) Internode (cm) TF0097rp1-1A-1 12.2 5.5 21.8 39.4 27.1 3.6 TF0097rp1-1A-2 11.1 5.3 18.3 34.7 28.6 3.8 TF0097rp1-1A-3 8.3 4.1 16.6 29.0 28.2 3.6 TF0097rp1-2A-1 9.1 3.3 21.9 34.3 35.5 2.6 TF0097rp1-2A-2 9.4 3.8 23.8 37.0 33.6 2.7 TF0097rp1-2A-3 8.2 3.2 21.0 32.4 30.6 2.7 TF0097rp1-2B-1 5.7 3.8 13.4 22.8 20.5 3.1 TF0097rp1-2B-2 10.5 5.3 21.6 37.4 25.2 3.8 TF0097rp1-2B-3 14.2 6.1 27.9 48.1 25.1 3.3 TF0097rp1-3A-1 15.3 7.2 28.9 51.4 28.5 3.6 TF0097rp1-3A-2 10.1 4.0 6.3 20.7 34.7 33.2 3.7 TF0097rp1-3A-3 14.6 6.6 11.3 28.2 49.4 27.0 3.6 TF0097rp1-4A-1 4.3 2.2 8.9 15.4 27.9 2.8 TF0097rp1-4A-2 5.1 2.5 13.1 20.6 28.7 3.5 TF0097rp1-4A-3 9.4 4.3 18.6 32.4 26.5 3.6 T89-01 5.1 2.2 13.1 20.4 39.9 2.7 T89-02 13.8 28.4 36.3 2.6

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T89-03 6.8 2.7 17.1 26.6 37.8 2.6 T89-04 6.9 2.7 17.3 27.0 37.1 2.4 T89-05 5.7 2.5 16.6 24.9 31.8 2.6 T89-06 6.1 2.7 8.2 18.4 27.1 31.7 2.7 T89-07 5.8 2.5 9.2 15.9 24.2 31.7 2.6 T89-08 3.9 2.1 16.1 22.0 29.2 2.6 T89-09 6.9 3.1 20.7 30.7 30.3 2.5 T89-10 5.5 2.5 10.3 16.6 24.6 36.9 2.6 T89-11 6.9 3.0 17.9 27.8 34.0 3.0 T89-12 6.6 3.0 15.2 24.8 31.9 2.8 T89-13 5.9 2.2 15.0 23.1 39.6 2.4 T89-14 3.4 1.7 13.2 18.3 32.7 2.5 T89-15 4.6 2.0 14.9 21.6 33.4 2.7 T89-16 6.1 2.7 17.7 26.4 31.8 2.6 T89-17 4.4 2.1 15.9 22.4 31.5 2.6 T89-18 5.6 2.6 15.0 23.3 32.7 2.7 T89-19 6.2 2.7 18.4 27.3 33.6 2.5 T89-20 5.9 2.7 8.4 16.5 25.2 35.9 2.7 T89-21 6.9 2.8 17.8 27.5 39.0 2.6 T89-22 5.4 2.2 13.7 21.3 37.9 2.7 T89-23 7.0 2.9 17.7 27.5 33.3 2.9 T89-24 6.6 2.7 17.3 26.6 33.8 2.6 T89-25 6.5 2.8 7.4 17.2 26.5 32.4 2.8 T89-26 4.7 2.2 6.2 14.3 21.2 33.0 2.8 T89-27 5.4 2.4 16.1 23.9 31.3 2.5 T89-28 3.6 1.5 11.7 16.7 39.5 2.5 T89-29 3.1 1.3 9.9 14.4 36.3 3.0 T89-30 5.0 2.1 13.7 20.8 36.2 2.9 T89-31 7.0 2.9 17.2 27.2 32.7 2.7 T89-32 6.0 2.5 16.7 25.2 32.3 2.5

[00348] Table 4.23 contains the dry weight ratios for the specified construction group relative to the corresponding wild type group of medium trunk, medium bark, medium root, medium leaf and average TOTAL. Table 4.23 also shows average SLA ratios and average internode length for the specified construction group relative to the corresponding wild type group.

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Table 4.23

Construction Group Medium Trunk Medium Bark Average Root Medium Sheet TOTAL Average Average SLA Medium internode TF0097rp1 1.74 1.82 1.07 1.28 1.43 0.83 1.35

[00349] Table 4.24 contains the dry weight ratios for the specified construction group relative to the corresponding wild type group of maximum trunk, maximum bark, maximum root, maximum leaf and maximum TOTAL.

Table 4.24

Construction Group Maximum trunk Maximum Bark Maximum Root Maximum Sheet TOTAL Maximum TF0097rp1 2.19 2.31 1.10 1.40 1.68

[00350] Table 4.25 contains the dry weight ratios for the lineage of the specified construction group relative to the corresponding wild type group of medium trunk, medium bark, medium root, medium leaf and average TOTAL. Table 4.25 also shows average SLA ratios and average internode length for the specified construction group relative to the corresponding wild type group.

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Medium Lineage Internode 1.37 1.00 1.29 1.36 1.25 Lineage Average SLA 0.82 0.97 0.69 CD CO o 00 o TOTAL Line Average- gem 1.42 1.43 1.49 1.87 0.94 Average Lineage Leaf 1.19 1.40 1.32 1.63 0.85 Average Lineage Root 1.07 Medium Lineage Trunk 2.02 1.41 2.06 2.41 1.22 Medium Lineage Trunk 1.86 1.58 1.79 2.36 1.11 Lineage of Group of Construction TF0097rp1-1A TF0097rp1-2A TF0097rp1-2B TF0097rp1-3A TF0097rp1-4A

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Construction Group TF0109 [00351] This construction induces an increase in biomass production. Dry weight measurements from the construction group show a 57% increase in the average trunk, 56% increase in the average bark, 34% increase in the average leaf and 40% increase in the average TOTAL compared to the corresponding wild type group. One of the strains in the construction group shows 82% increase in the average trunk, 62% increase in the average bark, 10% increase in the average leaf and 31% increase in the average TOTAL compared to the corresponding wild type group. For the strain where the dry root weight was measured, an increase in the sprout-root ratio was observed.

[00352] Table 4.26 contains dry weight data for the specified construction group and the corresponding wild type group.

Table 4.26

Construction Group Individual Trunk (9) Shell (g) Root (g) leaf (g) TOTAL (g) SLA (m ² / kg) Internode (cm) TF0109rp1-2A-1 10.5 4.2 25.5 40.2 29.0 2.8 TF0109rp1-2A-2 7.5 3.2 19.0 29.7 31.9 3.0 TF0109rp1-2A-3 7.7 3.3 21.9 32.9 30.4 2.7 TF0109rp1-2B-1 8.6 4.1 6.2 16.3 29.1 29.9 4.0 TF0109rp1-2B-2 11.4 4.3 6.5 19.8 35.5 32.6 3.9 TF0109rp1-2B-3 10.9 3.5 7.5 16.3 30.7 36.2 3.1 TF0109rp1-3B-1 9.0 3.9 21.4 34.3 38.5 2.8 TF0109rp1-3B-2 8.1 3.6 19.8 31.5 34.1 2.8 TF0109rp1-3B-3 8.6 3.6 22.0 34.1 31.6 2.9 TF0109rp1-4A-1 8.0 3.9 24.1 36.0 27.5 3.3 TF0109rp1-4A-2 8.3 4.0 24.6 37.0 33.3 3.1 TF0109rp1-4A-3 7.8 4.3 24.2 36.3 27.3 3.1 T89-01 5.1 2.2 13.1 20.4 39.9 2.7 T89-02 13.8 28.4 36.3 2.6

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T89-03 6.8 2.7 17.1 26.6 37.8 2.6 T89-04 6.9 2.7 17.3 27.0 37.1 2.4 T89-05 5.7 2.5 16.6 24.9 31.8 2.6 T89-06 6.1 2.7 8.2 18.4 27.1 31.7 2.7 T89-07 5.8 2.5 9.2 15.9 24.2 31.7 2.6 T89-08 3.9 2.1 16.1 22.0 29.2 2.6 T89-09 6.9 3.1 20.7 30.7 30.3 2.5 T89-10 5.5 2.5 10.3 16.6 24.6 36.9 2.6 T89-11 6.9 3.0 17.9 27.8 34.0 3.0 T89-12 6.6 3.0 15.2 24.8 31.9 2.8 T89-13 5.9 2.2 15.0 23.1 39.6 2.4 T89-14 3.4 1.7 13.2 18.3 32.7 2.5 T89-15 4.6 2.0 14.9 21.6 33.4 2.7 T89-16 6.1 2.7 17.7 26.4 31.8 2.6 T89-17 4.4 2.1 15.9 22.4 31.5 2.6 T89-18 5.6 2.6 15.0 23.3 32.7 2.7 T89-19 6.2 2.7 18.4 27.3 33.6 2.5 T89-20 5.9 2.7 8.4 16.5 25.2 35.9 2.7 T89-21 6.9 2.8 17.8 27.5 39.0 2.6 T89-22 5.4 2.2 13.7 21.3 37.9 2.7 T89-23 7.0 2.9 17.7 27.5 33.3 2.9 T89-24 6.6 2.7 17.3 26.6 33.8 2.6 T89-25 6.5 2.8 7.4 17.2 26.5 32.4 2.8 T89-26 4.7 2.2 6.2 14.3 21.2 33.0 2.8 T89-27 5.4 2.4 16.1 23.9 31.3 2.5 T89-28 3.6 1.5 11.7 16.7 39.5 2.5 T89-29 3.1 1.3 9.9 14.4 36.3 3.0 T89-30 5.0 2.1 13.7 20.8 36.2 2.9 T89-31 7.0 2.9 17.2 27.2 32.7 2.7 T89-32 6.0 2.5 16.7 25.2 32.3 2.5

[00353] Tabe a 4.27 contains the dry weight ratios for the specified construction group relative to the corresponding wild type group of medium trunk, medium bark, medium root, medium leaf and

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TOTAL average. Table 4.27 also shows average SLA ratios and average internode length for the specified construction group relative to the corresponding wild type group.

Table 4.27

Construction Group Medium Trunk Medium Bark Average Root leaf Average TOTAL Medium Average SLA Medium internode TF0109rp1 1.57 1.56 0.81 1.34 1.40 0.93 1.18 [00354] Table 4 L28 con have dry weight ratios for the group

specified construction type for the corresponding wild type group of maximum trunk, maximum bark, maximum root, maximum leaf and maximum TOTAL.

Table 4.28

Construction Group Maximum trunk Maximum Bark Maximum Root Maximum Sheet TOTAL Maximum TF0109rp 1 1.63 1.39 0.74 1.23 1.31

[00355] Table 4.29 contains the dry weight ratios for the specified building group strain relative to the corresponding wild type group of medium trunk, medium bark, medium root, medium leaf and average TOTAL. Table 4.29 also shows average SLA ratios and average internode length for the specified construction group relative to the corresponding wild type group.

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Medium Line Line 1.06 1.39 1.07 1.19 Lineage Average SLA 0.89 0.96 1.01 CD 00 o TOTAL Line Average- gem 1.41 1.31 1.38 1.50 Average Lineage Leaf 1.39 1.10 1.32 1.53 Average Lineage Root CO o Average Lineage Bark m 1.62 1.51 1.66 Medium Lineage Trunk 1.51 1.82 1.51 1.42 Lineage of Group of Construction TF0109rp1-2A TF0109rp1-2B TF0109rp1-3B TF0109rp1-4A

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Construction Group TFSTT019 [00356] This construction induces an increase in biomass production. Measurements of dry weight in the construction group show 19% increase in the average trunk, 12% increase in the average bark, 10% increase in the average leaf and 11% increase in the average TOTAL compared to the corresponding wild type group. This gene also leads to an increased SLA in many strains, which in many cases are coupled for effective growth.

[00357] Table 4.30 contains dry weight data for the specified construction group and the corresponding wild type group.

Table 4.30

Construction Group Individual Trunk (9) Shell (g) Root (g) leaf (g) TOTAL (g) SLA (m ² / kg) Internode (cm) TFSTT019rp1-2A-1 5.1 2.1 15.0 22.2 39.0 2.5 TFSTT019rp1-2A-2 18.7 18.7 35.4 2.7 TFSTT019rp1-2A-3 5.8 2.5 16.9 25.1 40.0 2.6 TFSTT019rp1-3A-1 6.3 2.6 18.6 27.5 41.1 2.6 TFSTT019rp1-3A-2 2.2 1.2 11.2 14.5 30.9 2.1 TFSTT019rp1-3A-3 9.0 3.4 22.9 35.3 33.1 2.7 TFSTT019rp1-4BA-1 5.9 2.4 15.7 24.0 35.5 2.6 TFSTT019rp1-4BA-2 7.6 3.1 19.3 29.9 35.8 2.6 TFSTT019rp1-4BA-3 TFSTT019rp1-4BB-1 6.0 2.5 15.9 24.4 40.4 2.6 TFSTT019rp1-4BB-2 5.7 2.4 17.6 25.6 40.0 2.2 TFSTT019rp1-4BB-3 6.0 2.3 16.4 24.7 39.9 2.5 T89-01 5.1 2.2 13.1 20.4 39.9 2.7 T89-02 13.8 28.4 36.3 2.6 T89-03 6.8 2.7 17.1 26.6 37.8 2.6 T89-04 6.9 2.7 17.3 27.0 37.1 2.4 T89-05 5.7 2.5 16.6 24.9 31.8 2.6 T89-06 6.1 2.7 8.2 18.4 27.1 31.7 2.7

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T89-07 5.8 2.5 9.2 15.9 24.2 31.7 2.6 T89-08 3.9 2.1 16.1 22.0 29.2 2.6 T89-09 6.9 3.1 20.7 30.7 30.3 2.5 T89-10 5.5 2.5 10.3 16.6 24.6 36.9 2.6 T89-11 6.9 3.0 17.9 27.8 34.0 3.0 T89-12 6.6 3.0 15.2 24.8 31.9 2.8 T89-13 5.9 2.2 15.0 23.1 39.6 2.4 T89-14 3.4 1.7 13.2 18.3 32.7 2.5 T89-15 4.6 2.0 14.9 21.6 33.4 2.7 T89-16 6.1 2.7 17.7 26.4 31.8 2.6 T89-17 4.4 2.1 15.9 22.4 31.5 2.6 T89-18 5.6 2.6 15.0 23.3 32.7 2.7 T89-19 6.2 2.7 18.4 27.3 33.6 2.5 T89-20 5.9 2.7 8.4 16.5 25.2 35.9 2.7 T89-21 6.9 2.8 17.8 27.5 39.0 2.6 T89-22 5.4 2.2 13.7 21.3 37.9 2.7 T89-23 7.0 2.9 17.7 27.5 33.3 2.9 T89-24 6.6 2.7 17.3 26.6 33.8 2.6 T89-25 6.5 2.8 7.4 17.2 26.5 32.4 2.8 T89-26 4.7 2.2 6.2 14.3 21.2 33.0 2.8 T89-27 5.4 2.4 16.1 23.9 31.3 2.5 T89-28 3.6 1.5 11.7 16.7 39.5 2.5 T89-29 3.1 1.3 9.9 14.4 36.3 3.0 T89-30 5.0 2.1 13.7 20.8 36.2 2.9 T89-31 7.0 2.9 17.2 27.2 32.7 2.7 T89-32 6.0 2.5 16.7 25.2 32.3 2.5

[00358] Table 4.31 contains the dry weight ratios of the specified construction group in relation to the corresponding wild type group of medium trunk, medium bark, medium root, medium leaf and average TOTAL. Table 4.31 also shows average SLA ratios and average internode length for the specified construction group relative to the corresponding wild type group.

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Table 4.31

Construction Group Medium Trunk Medium Bark Average Root leaf Average TOTAL Average Average SLA Medium internode TFSTT019r P1 1.05 1.00 1.08 1.02 1.09 0.95

[00359] Table 4.32 contains the dry weight ratios for the specified construction group with respect to the corresponding wild type group for maximum trunk, maximum bark, maximum leaf and maximum TOTAL.

Table 4.32

Construction Group Maximum trunk Maximum Bark Maximum Root Maximum Sheet TOTAL Maximum TFSTT019r P1 1.28 1.10 1.11 1.15

[00360] Table 4.33 contains the dry weight ratios for the lineage of the specified construction group relative to the corresponding wild type group of medium trunk, medium bark, medium root, medium leaf and average TOTAL. Table 4.33 also shows average SLA ratios and average internode length for the specified construction group relative to the corresponding wild type group.

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Medium Lineage Internode 0.98 0.93 0.97 0.91 Lineage Average SLA 1.11 1.02 1.04 1.17 TOTAL Average Lineage 0.91 1.06 1.11 1.03 Average Lineage Leaf 1.06 1.10 1.10 1.05 Average Lineage Root Average Lineage Bark 0.93 0.98 1.12 0.98 Medium Lineage Trunk 0.97 1.03 1.19 1.04 Construction Group Lineage TFSTT019rp1-2A TFSTT019rp1-3A TFSTT019rp1-4BA TFSTT019rp1-4BB

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Construction Group TFSTT051 [00361] This construction induces an increase in biomass production. Measurements of dry weight of the construction group of one of the lines of the construction group show 22% increase in the average trunk, 30% increase in the average bark, 29% increase in the average leaf and 26% increase in the average TOTAL in comparison with the corresponding wild type group. Table 4.34 contains dry weight data for the specified construction group and the corresponding wild type group.

Table 4.34

Construction Group Individual Trunk (9) Shell (g) Root (g) leaf (g) TOTAL (g) SLA (m ² / kg) Internode (cm) TFSTT051rp1-3B-1 4.6 2.4 12.4 19.4 24.6 2.1 TFSTT051rp1-3B-2 4.0 2.3 11.4 17.6 24.1 2.0 TFSTT051rp1-3B-3 4.6 2.6 13.1 20.3 22.5 2.0 TFSTT051rp1-4A-1 6.7 2.9 20.1 29.7 30.5 2.9 TFSTT051rp1-4A-2 6.1 2.9 18.5 27.4 33.3 2.8 TFSTT051rp1-4A-3 8.0 3.7 22.9 34.6 31.8 2.5 T89-01 5.1 2.2 13.1 20.4 39.9 2.7 T89-02 13.8 28.4 36.3 2.6 T89-03 6.8 2.7 17.1 26.6 37.8 2.6 T89-04 6.9 2.7 17.3 27.0 37.1 2.4 T89-05 5.7 2.5 16.6 24.9 31.8 2.6 T89-06 6.1 2.7 8.2 18.4 27.1 31.7 2.7 T89-07 5.8 2.5 9.2 15.9 24.2 31.7 2.6 T89-08 3.9 2.1 16.1 22.0 29.2 2.6 T89-09 6.9 3.1 20.7 30.7 30.3 2.5 T89-10 5.5 2.5 10.3 16.6 24.6 36.9 2.6 T89-11 6.9 3.0 17.9 27.8 34.0 3.0 T89-12 6.6 3.0 15.2 24.8 31.9 2.8 T89-13 5.9 2.2 15.0 23.1 39.6 2.4

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T89-14 3.4 1.7 13.2 18.3 32.7 2.5 T89-15 4.6 2.0 14.9 21.6 33.4 2.7 T89-16 6.1 2.7 17.7 26.4 31.8 2.6 T89-17 4.4 2.1 15.9 22.4 31.5 2.6 T89-18 5.6 2.6 15.0 23.3 32.7 2.7 T89-19 6.2 2.7 18.4 27.3 33.6 2.5 T89-20 5.9 2.7 8.4 16.5 25.2 35.9 2.7 T89-21 6.9 2.8 17.8 27.5 39.0 2.6 T89-22 5.4 2.2 13.7 21.3 37.9 2.7 T89-23 7.0 2.9 17.7 27.5 33.3 2.9 T89-24 6.6 2.7 17.3 26.6 33.8 2.6 T89-25 6.5 2.8 7.4 17.2 26.5 32.4 2.8 T89-26 4.7 2.2 6.2 14.3 21.2 33.0 2.8 T89-27 5.4 2.4 16.1 23.9 31.3 2.5 T89-28 3.6 1.5 11.7 16.7 39.5 2.5 T89-29 3.1 1.3 9.9 14.4 36.3 3.0 T89-30 5.0 2.1 13.7 20.8 36.2 2.9 T89-31 7.0 2.9 17.2 27.2 32.7 2.7 T89-32 6.0 2.5 16.7 25.2 32.3 2.5

[00362] Table 4.35 contains the dry weight ratios of the specified construction group relative to the corresponding wild type group of medium trunk, medium bark, medium root, medium leaf and average TOTAL. Table 4.35 also shows average SLA ratios and average internode length for the specified construction group relative to the corresponding wild type group.

Table 4.35

Construction Group Medium Trunk Medium Bark Average Root leaf Average TOTAL Medium Average SLA Medium internode TFSTT05rp 1 1.00 1.15 1.03 1.03 0.81 0.90 [00363] A Ί fabela 4.36 contains the reasons for and dry weight for the group

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298/362 of construction specified for the corresponding wild type group for maximum trunk, maximum bark, maximum root, maximum leaf and maximum TOTAL.

Table 4.36

Construction Group Maximum trunk Maximum Bark Maximum Root Maximum Sheet TOTAL Maximum TFSTT051rp1 1.15 1.20 1.11 1.13

[00364] Table 4.36 contains the dry weight ratios for the lineage of the specified construction group with respect to the corresponding wild type group of medium trunk, medium bark, medium leaf and average TOTAL. Table 4.37 also shows average SLA ratios and average intern length of the lineage of the specified construction group relative to the corresponding wild type group.

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Medium Lineage Internode 0.76 1.04 Lineage Average SLA 0.69 0.93 TOTAL Average Lineage 0.79 1.26 Average Lineage Leaf 1 ^ 1 ^ O 1.29 Average Lineage Root Average Lineage Bark 1.00 1.30 Medium Lineage Trunk 1 ^ 1 ^ O 1.22 Lineage of Group of construction TFSTT051rp1-3B TFSTT051rp1-4A

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Example 5

Density Measurement [00365] A 5 cm long section of the trunk (the segment between 36 cm and 41 cm from the soil) of each plant was stored in a freezer (-20 ° C) after harvest. The samples subjected to the density measurement were first thawed and peeled and then the central core was removed. Weight (w) was measured using a scale and volume (v) was determined using Archimedes' principle, the wood samples were pushed (using a needle) into a beaker (placed on a scale) with water and the weight increase is equivalent to the weight of the water pushed away by the wood sample and since the water density is (1 g / cm ³ ), it is equivalent to the volume of the wood samples. The samples were then dried in the oven for> 48 hours at 45 ° C. Dry weight (dw) was measured and density (d) was calculated according to (1).

D = dw / v [00366] The samples for each construction are compared to samples of the wild type (T89) from the same cultivation cycle. Each building must fulfill two criteria to be seen as a building group with altered density.

[00367] Significant differences in mean density according to a t test (p-value <0.01). The t test is on both sides and assuming uneven variance.

[00368] Two or more individuals outside (on the same side) of a 95% confidence interval around the wild type population.

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Summary of Density Table

Average KR Average T89 Mean (KR) / Mean to (T89) T test 99% Samples Above 95% conf int Change Means TF0002Rp2 0.335 0.284 1,179 Signal 4 YES TF0003 0.291 0.255 1,140 Signal 2 YES TF0011 0.314 0.284 1.106 Signal 2 YES TF0013 0.252 0.260 0.970 No signal 0 NO TF0045 0.306 0.274 1,117 Signal 2 YES TF0052 0.302 0.275 1,099 No signal 2 NO TF0065 0.273 0.260 1,051 No signal 1 NO TF0076 0.267 0.260 1,027 No signal 0 NO TF0076Rp2 0.285 0.284 1.002 No signal 1 NO TF0096 0.307 0.274 1,121 No signal 1 NO TF0097Rp1 0.314 0.284 1.107 Signal 7 YES TF0104 0.284 0.274 1,036 No signal 0 NO TF0109Rp1 0.286 0.284 1.007 No signal 3 NO* TF0116 0.277 0.275 1.008 No signal 0 NO TF0132.2nd 0.294 0.274 1,071 No signal 1 NO TF0132rp1 0.272 0.255 1,068 Signal 4 YES TF0146 0.318 0.274 1,161 Signal 3 YES TF0173 0.256 0.254 1.008 No signal 0 NO TF0247 0.294 0.274 1,073 No signal 1 NO TF0405 0.287 0.274 1,045 No signal 0 NO TFSTT004 0.284 0.275 1,033 No signal 0 NO TFSTT013 0.319 0.284 1,123 Signal 2 YES TFSTT016 0.270 0.275 0.980 No signal 0 NO TFSTT019 0.257 0.260 0.989 No signal 0 NO TFSTT035 0.249 0.260 0.957 No signal 0 NO TFSTT036 0.306 0.274 1,117 Signal 2 YES TFSTT038 0.295 0.275 1,073 No signal 0 NO TFSTT045 0.274 0.274 1,000 No signal 1 NO TFSTT051 0.278 0.275 1,013 No signal 0 NO

*) The construction group TF109 (Replante 1) does not meet the criteria for altered density, but the strains of the construction group; TF0109Rp1-2B (+ 18%) and TF109Rp1-4A (-16%) fill.

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Explanation of the summary of the density tables of the construction group.

[00369] All densities are given in g / cm ³

TFXXX TFXXX Average: Construction group average Maximum TFXXX: Construction group maximum Minimum TFXXX: Construction Group Minimum TFXXX number: Sample number for group building Number of TFXXX lines: Number of lines (only when the number of lines differs from the number of samples)

T89 Average: Average wild type Maximum T89: Wild-type maxim Minimum T89: Minimum wild type T89 Number: Number of wild type samples Confidence interval (95%) (Wild-type mean) +/- (t _ta beia (295% ta // ed) * Standard deviation for wild type samples)

T test T test TFXXX number> upper limit of Cl Number of TFXXX samples outside the upper limit of the Confidence Interval TFXXX number <lower limit Cl Number of TFXXX samples outside the lower limit of the Confidence Interval Average (TFXXX) / Average (T89) Construction group average / Wild type average Maximum (TFXXX) / Maximum (T89) Construction group maximum / Wild type maximum

[00370] The following construction groups did not generate any

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303/362 data from TF0089, TF0097, TF0109, TF0132 and TFSTT047.

Construction group TF002 (replant 2)

TF002Rp2 density raw data

TF0002Rp2 Density Name of individual: (g / cm ³ ) TF0002Rp2-1B-1 0.317 TF0002Rp2-1 B-2 0.360 TF0002Rp2-1B-3 0.323 TF0002Rp2-2A-1 0.322 TF0002Rp2-2A-2 0.323 TF0002Rp2-2A-3 0.366 TF0002Rp2-3B-1 0.330 TF0002Rp2-3B-2 0.321 TF0002Rp2-3B-3 0.352

Summary of TF002Rp2 density

TF0002Rp2 TF0002Rp2 Average: 0.335 TF0002Rp2 Maximum: 0.366 TF0002Rp2 Minimum: 0.317 TF0002Rp2 Number: 9 Number of TF0002Rp2 strains: 3

T89 Average: 0.284 Maximum T89: 0.338 Minimum T89: 0.252 T89 Number: 32 Confidence interval (95%) 0.284 +/- 0.041

T test 4.066E-08 TF0002Rp2 number> 0.325 4 TF0002Rp2 number <0.243 0 Average (TF0002Rp2) / Average (T89) 1,179 Maximum (TF0002Rp2) / Maximum (T89) 1,083 [00371] TF0002Rp2 has density greater significant (according to

with criteria 1 and 2) (+ 18% on average) that the T89 group corresponds to Petition 870170074044, of 29/09/2017, p. 315/378

304/362 tooth. The density change (compared to T89) for the 3 strains in the TF0002Rp2 construction group (3 individuals of each strain), TF0002Rp2-1B (+ 17% on average), TF0002Rp2-2A (+ 19% on average) and TF0002Rp2-3B (+ 18% on average). The TF0002Rp2-3B strain itself fulfills criteria 1 and 2.

Construction group TF0003

TF0003 raw density data

TF0003 Density Name of individual: (g / cm ³ ) TF0003-1A 0.318 TF0003-1B 0.323 TF0003-2A 0.242 TF0003-3A TF0003-3B TF0003-4A TF0003-4B 0.282

Summary of TF0003 density

TF0003 TF0003 Average: 0.291 TF0003 Maximum: 0.323 TF0003 Minimum: 0.242 TF0003 Number: 4 Number of TF0003 strains:

T89 Average: 0.255 Maximum T89: 0.313 Minimum T89: 0.221 T89 Number: 39 Confidence interval (95%) 0.255 +/- 0.045

T test 6,712E-03 TF0003 number> 0.301 2 TF0003 number <0.21 0 Average (TF0003) / Average (T89) 1,140 Maximum (TF0003) / Maximum (T89) 1,032

[00372] The TF0003 construction group has significant density. Petition 870170074044, of 29/09/2017, p. 316/378

305/362 higher (according to criteria 1 and 2) (+ 14% on average) than the corresponding T89 group.

Construction group TF0011

TF0011 raw density data

TF0011 Density Name of individual: (g / cm ³ ) TF0011-1A-1 0.308 TF0011-1A-2 0.360 TF0011-1B 0.264 TF0011-2A-1 0.337 TF0011-2A-2 0.298 TF0011-3A-1 0.326 TF0011-3A-2 0.350 TF0011-3B-1 0.333 TF0011-3B-2 0.303 TF0011-4 ³ 0.261

Summary of density of TF0011

TF0011 TF0011 Average: 0.314 TF0011 Maximum: 0.360 TF0011 Minimum: 0.261 TF0011 Number: 10 Number of TF0011 strains:

T89 Average: 0.284 Maximum T89: 0.361 Minimum T89: 0.222 T89 Number: 41 Confidence interval (95%) 0.284 +/- 0.06

T test 7,377E-03 TF0011 number> 0.344 2 TF0011 number <0.224 0 Average (TF0011) / Average (T89) 1.106 Maximum (TF0011) / Maximum (T89) 0.997

[00373] The TF0011 construction group has significantly higher density (according to criteria 1 and 2) (+ 11% on average) than the group

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Τ89 corresponding.

Construction group TF0013

TF0013 raw density data

TF0013 Density Name of individual: (g / cm ³ ) TF0013-1A-1 0.246 TF0013-1A-2 0.236 TF0013-2A TF0013-2B 0.265 TF0013-3A 0.258 TF0013-3BA 0.254 TF0013-3BB 0.245 TF0013-4BA 0.258 TF0013-4BB 0.253

Summary of density of TF0013

TF0013 TF0013 Average: 0.252 TF0013 Maximum: 0.265 TF0013 Minimum: 0.236 TF0013 Number: 8 Number of TF0013 strains:

T89 Average: 0.260 Maximum T89: 0.358 Minimum T89: 0.218 T89 Number: 45 Confidence interval (95%) 0.26 +/- 0.049

T test 3.760E-01 TF0013 number> 0.309 0 TF0013 number <0.211 0 Average (TF0013) / Average (T89) 0.970 Maximum (TF0013) / Maximum (T89) 0.740

[00374] The TF0013 construction group has no significant density difference (according to criteria 1 and 2) compared to the corresponding T89 group.

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Construction group TF0045

Raw density data of TF0045

TF0045 Density Name of individual: (g / cm ³ ) TF0045-1A-1 0.344 TF0045-1A-2 TF0045-1A-3 0.270 TF0045-1B-1 0.320 TF0045-1B-2 0.323 TF0045-1B-3 0.328 TF0045-2B-1 0.303 TF0045-2B-3 0.255

Summary of density of TF0045

TF0045 TF0045 Average: 0.306 TF0045 Maximum: 0.344 TF0045 Minimum: 0.255 TF0045 Number: 7 Number of lines TF0045: 3

T89 Average: 0.274 Maximum T89: 0.354 Minimum T89: 0.226 T89 Number: 36 Confidence interval (95%) 0.274 +/- 0.052

T test 5,760E-03 TF0045 number> 0.326 2 TF0045 number <0.222 0 Average (TF0045) / Average (T89) 1,117 Maximum (TF0045) / Maximum (T89) 0.972

[00375] The TF0045 construction group has significant density. Petition 870170074044, of 29/09/2017, p. 319/378

308/362 significantly higher (according to criteria 1 and 2) (+ 12% on average) than the corresponding T89 group. The density change (compared to T89) for the 3 strains in the TF0045 construction group, TF0045-1A (+ 12% on average (2 individuals measured)), TF0045-1B (+ 18% on average (3 individuals) and TF0045-2B (+ 2% on average (2 individuals)).

Construction group TF0052

TF0052 raw density data

TF0052 Density Name of individual: (g / cm ³ ) TF0052-1A 0.241 TF0052-1B 0.346 TF0052-2A 0.323 TF0052-2B 0.251 TF0052-3A 0.393 TF0052-3B 0.278 TF0052-4A 0.261 TF0052-4B 0.322

Summary of density of TF0052

TF0052 TF0052 Average: 0.302 TF0052 Maximum: 0.393 TF0052 Minimum: 0.241 TF0052 Number: 8 Number of TF0052 strains:

T89 Average: 0.275 Maximum T89: 0.345 Minimum T89: 0.223 T89 Number: 23 Confidence interval (95%) 0.275 +/- 0.069

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T test 9,876E-02 TF0052 number> 0.343 2 TF0052 number <0.206 0 Average (TF0052) / Average (T89) 1,099 Maximum (TF0052) / Maximum (T89) 1,139

[00376] The TF0052 construction group has no significant density difference (according to criterion 1) compared to the corresponding T89 group. Although TF0052 has an increased density (+ 10%) on average and satisfies criterion 2.

Construction group TF0065

TF0065 raw density data

TF0065 Density Name of individual: (g / cm ³ ) TF0065-1AA 0.333 TF0065-1AB 0.245 TF0065-1BA 0.267 TF0065-1BB 0.253 TF0065-2B 0.278 TF0065-3A 0.262 TF0065-4B 0.272

Summary of density of TF0065

TF0065 TF0065 Average: 0.273 TF0065 Maximum: 0.333 TF0065 Minimum: 0.245 TF0065 Number: 7 Number of TF0065 strains:

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T89 Average: 0.260 Maximum T89: 0.358 Minimum T89: 0.218 T89 Number: 45 Confidence interval (95%) 0.26 +/- 0.049

T test 2.012E-01 TF0065 number> 0.309 1 TF0065 number <0.211 0 Average (TF0065) / Average (T89) 1,051 Maximum (TF0065) / Maximum (T89) 0.931

[00377] The TF0065 construction group has no significant density difference (according to criteria 1 and 2) compared to the corresponding T89 group.

Construction group TF0076

TF0076 raw density data

TF0076 Density Name of Individual: (g / cm ³ ) TF0076-2AA 0.250 TF0076-2AB 0.253 TF0076-3BA 0.252 TF0076-3BB 0.257 TF0076-4B TF0076-5BA 0.288 TF0076-5BB 0.300

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Summary of density of TF0065

TF0076 TF0076 Average: 0.267 TF0076 Maximum: 0.300 TF0076 Minimum: 0.250 TF0076 Number: 6 Number of TF0076 lines:

T89 Average: 0.260 Maximum T89: 0.358 Minimum T89: 0.218 T89 Number: 45 Confidence interval (95%) 0.26 +/- 0.049

T test 5,054E-01 TF0076 number> 0.309 0 TF0076 number <0.211 0 Average (TF0076) / Average (T89) 1,027 Maximum (TF0076) / Maximum (T89) 0.839

[00378] The TF0076 construction group has no significant density difference (according to criteria 1 and 2) compared to the corresponding T89 group.

Construction group TF20076 (Replante 2)

Raw density data of TF0076Rp2

TF0076Rp2 Density Name of Individual: (g / cm ³ ) TF0076Rp2-3BB-1 0.237 TF0076Rp2-3BB-2 0.311 TF0076Rp2-3BB-3 0.274 TF0076Rp2-4B-1 0.304 TF0076Rp2-4B-2 0.293 TF0076Rp2-4B-3 0.301

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TF0076Rp2-5BA-1 0.262 TF0076Rp2-5BA-2 0.288 TF0076Rp2-5BA-3 0.259 TF0076Rp2-5BB-1 0.267 TF0076Rp2-5BB-2 0.263 TF0076Rp2-5BB-3 0.356

Summary of density of TF0076Rp2

TF0076Rp2 TF0076Rp2 Average: 0.285 TF0076Rp2 Maximum: 0.356 TF0076Rp2 Minimum: 0.237 TF0076Rp2 Number: 12 Number of TF0076Rp2 strains: 4

T89 Average: 0.284 Maximum T89: 0.338 Minimum T89: 0.252 T89 Number: 32 Confidence interval (95%) 0.284 +/- 0.041

T test 9.407E-01 TF0076Rp2 number> 0.325 1 TF0076Rp2 number <0.243 1 Average (TF0076Rp2) / Average (T89) 1.002 Maximum (TF0076Rp2) / Maximum (T89) 1,054

[00379] The TF0076Rp2 construction group has no significant difference in density (according to criteria 1 and 2) compared to the corresponding T89 group.

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Construction group T0096

TF0096 raw density data

TF0096 Density Name of individual: (g / cm ³ ) TF0096-2A 0.296 TF0096-2B 0.249 TF0096-3A 0.321 TF0096-3B 0.308 TF0096-4A 0.363

Summary of density of TF0096

TF0096 TF0096 Average: 0.307 TF0096 Maximum: 0.363 TF0096 Minimum: 0.249 TF0096 Number: 5 Number of TF0096 strains:

T89 Average: 0.274 Maximum T89: 0.354 Minimum T89: 0.226 T89 Number: 36 Confidence interval (95%) 0.274 +/- 0.052

T test 1,562E-02 TF0096 number> 0.326 1 TF0096 number <0.222 0 Average (TF0096) / Average (T89) 1,121 Maximum (TF0096) / Maximum (T89) 1,024

[00380] The TF0096 construction group has no significant difference in density (according to criteria 1 and 2) compared to the corresponding T89 group.

Construction group TF0097 (replant 1)

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Raw density data of TF0097Rp1

TF0097Rp1 Density TF0097Rp1 Density Name of individual: (g / cm ³ ) Name of individual: (g / cm ³ ) TF0097Rp1-1A-1 0.327 TF0097Rp1-2B-2 0.351 TF0097Rp1-1A-2 0.335 TF0097Rp1-2B-3 0.309 TF0097Rp1-1A-3 0.344 TF0097Rp1-3A-1 0.309 TF0097Rp1-2A-1 0.275 TF0097Rp1-3A-2 0.268 TF0097Rp1-2A-2 0.284 TF0097Rp1-3A-3 0.297 TF0097Rp1-2A-3 0.308 TF0097Rp1-4A-1 0.333 TF0097Rp1-2B-1 0.328 TF0097Rp1-4A-2 0.325 TF0097Rp1-4A-3 0.319

Summary of density of TF0097Rp1

TF0097Rp1 TF0097Rp1 Average: 0.314 TF0097Rp1 Maximum: 0.351 TF0097Rp1 Minimum: 0.268 TF0097Rp1 Number: 15 Number of TF097Rp1 strains: 5

T89 Average: 0.284 Maximum T89: 0.338 Minimum T89: 0.252 T89 Number: 32 Confidence interval (95%) 0.284 +/- 0.041

T test 4.607E-05 TF0097Rp1 number> 0.325 7 TF0097Rp1 number <0.243 0 Average (TF0097Rp1) / Average (T89) 1.107 Maximum (TF0097Rp1) / Maximum (T89) 1,038

[00381] TF0097Rp1 has a significantly higher density (according to criteria 1 and 2) (+ 11% on average) than the T89 group corresponds to Petition 870170074044, of 29/09/2017, p. 326/378

Pondering 315/362. The density change (compared to T89) for the 5 strains in the TF0097Rp1 construction group (3 individuals of each strain), TF00097Rp1-1A (+ 18% on average), TF00097Rp1-2A (+ 2% on average), TF00097Rp1-2B (+ 16% on average), TF00097Rp1-3A (+ 3% on average) and TF00097Rp1-4A (+ 15% on average). The strains TF00097Rp1-1A, TF00097Rp1-2B and TF009Rp1-2B and TF00097Rp1-4A themselves meet criteria 1 and 2.

Construction group TF0104

TF0104 raw density data

TF0104 Density Name of individual: (g / cm ³ ) TF0104-1A 0.282 TF0104-1B 0.282 TF0104-2A 0.297 TF0104-3A 0.298 TF0104-3B 0.261

Summary of density of TF0104

TF0104 TF0104 Average: 0.284 TF0104 Maximum: 0.298 TF0104 Minimum: 0.261 TF0104 Number: 5 Number of TF0104 strains:

T89 Average: 0.274 Maximum T89: 0.354 Minimum T89: 0.226 T89 Number: 36 Confidence interval (95%) 0.274 +/- 0.052

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T test 4,063E-01 TF0104 number> 0.326 0 TF0104 number <0.222 0 Average (TF0104) / Average (T89) 1,036 Maximum (TF0104) / Maximum (T89) 0.842

[00382] The TF0104 construction group has no significant difference in density (according to criteria 1 and 2) compared to the corresponding T89 group.

Construction group TF0109 (Replant 1)

Raw TF0109Rp1 density data

TF0109Rp1 Density TF0109Rp1 Density Name of individual: (g / cm ³ ) Name of individual: (g / cm ³ ) TF0109Rp1-2A-1 0.301 TF0109Rp1-3B-1 0.279 TF0109Rp1-2A-2 0.294 TF0109Rp1-3B-2 0.279 TF0109Rp1-2A-3 0.284 TF0109Rp1-3B-3 0.271 TF0109Rp1-2B-1 0.329 TF0109Rp1-4A-1 0.253 TF0109Rp1-2B-2 0.342 TF0109Rp1-4A-2 0.238 TF0109Rp1-2B-3 0.336 TF0109Rp1-4A-3 0.222

Summary of density of TF0109Rp1

TF0109Rp1 TF0109Rp1 Average: 0.286 TF0109Rp1 Maximum: 0.342 TF0109Rp1 Minimum: 0.222 TF0109Rp1 Number: 12 Number of strains TF0109Rp1: 4

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T89 Average: 0.284 Maximum T89: 0.338 Minimum T89: 0.252 T89 Number: 32 Confidence interval (95%) 0.284 +/- 0.041

T test 8.314E-01 TF0109Rp1 number> 0.325 3 TF0109Rp1 number <0.243 2 Average (TF0109Rp1) / Average (T89) 1.007 Maximum (TF0109Rp1) / Maximum (T89) 1,014

[00383] The TF0109Rp1 construction group has no significant difference in density (according to criteria 1 and 2) compared to the corresponding T89 group. The density change (compared to T89) for the 4 lines of the TF0109Rp1 construction group (3 individuals of each line), TF010Rp1-2A (+ 3% on average), TF0109Rp1-2B (+ 18% on average), TF0109Rp1-3B (-3% on average and TF0109Rp1-4A (-16 on average). The TF0109Rp1-2B and TF0109Rp1-4A strains fulfill criteria 1 and 2 themselves.

Construction group TF0116

TF0116 raw density data

TF0116 Density Name of individual: (g / cm ³ ) TF0116-1B TF0116-2A 0.306 TF0116-2B-1 0.293 TF0116-2B-2 0.263 TF0116-4A 0.292

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TF0116-5B 0.247

TF0116-6A 0.271 TF0116-6B 0.268 Summary of density of TF0116 TF0116 TF0116 Average: 0.277 TF0116 Maximum: 0.306 TF0116 Minimum: 0.247 TF0116 number: 7 Number of TF0116 strains:

T89 Average: 0.275 Maximum T89: 0.345 Minimum T89: 0.223 T89 Number: 23 Confidence interval (95%) 0.275 +/- 0.069

T test 8,725E-01 TF0116 number> 0.343 0 TF0116 number <0.206 0 Average (TF0116) / Average (T89) 1.008 Maximum (TF0116) / Maximum (T89) 0.887

[00384] The TF0116 construction group has no significant difference in density (according to criteria 1 and 2) compared to the corresponding T89 group.

Construction group TF0132 (2nd set of lineages of construction groups)

TF0132.2nd raw density data

TF0132.2nd Density Name of individual: (g / cm ³ ) TF0132.2nd-1A 0.263 TF0132.2nd-1B 0.284

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TF0132.2nd-2A 0.350 TF0132.2nd-4B 0.315 TF0132.2nd-5A 0.271 TF0132.2nd-5B 0.286 TF0132.2nd-6B 0.285 TF0132.2nd-7A 0.294

Summary of density of TF0132.2nd

TF0132.2nd TF0132.2nd Average: 0.294 TF0132.2nd Maximum: 0.350 TF0132.2nd Maximum: 0.263 TF0132.2nd number: 8 Number of lines TF0132.2nd:

T89 Average: 0.274 Maximum T89: 0.354 Minimum T89: 0.226 T89 Number: 36 Confidence interval (95%) 0.274 +/- 0.052

T test 6,257E-02 TF0132.2nd number> 0.326 1 TF0132.2nd number <0.222 0 Average (TF0132.2nd) / Average (T89) 1,071 Maximum (TF0132.2nd) / Maximum (T89) 0.988

[00385] The TF032.2nd construction group has no significant difference in density (according to criteria 1 and 2) compared to the corresponding T89 group.

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Construction group TF0132 (Replant 1)

TF0132rp1 density raw data

TF0132rp1 Density TF0132rp1 Density Name of individual: (g / cm ³ ) Name of individual: (g / cm ³ ) TF0132Rp1-1B-1 0.274 TF0132Rp1-4AC-3 0.278 TF0132Rp1-1B-2 0.265 TF0132Rp1-4B-1 0.291 TF0132Rp1-1B-3 0.265 TF0132Rp1-4B-2 0.260 TF0132Rp1-3BB-1 0.260 TF0132Rp1-4B-3 0.282 TF0132Rp1-3BB-2 0.265 TF0132Rp1-6B-1 0.270 TF0132Rp1-3BB-3 0.267 TF0132Rp1-6B-2 0.281 TF0132Rp1-4AC-1 0.283 TF0132Rp1-6B-3 0.269 TF0132Rp1-4AC-2 0.275 Summary of density ede TF0132Rp1

TF0132rp1 TF0132rp1 Average: 0.272 TF0132rp1 Maximum: 0.291 TF0132rp1 Minimum: 0.260 TF0132rp1 Number: 15 Number of lines TF0132rp1: 5

T89 Average: 0.255 Maximum T89: 0.275 Minimum T89: 0.232 T89 Number: 27 Confidence interval (95%) 0.255 +/- 0.025

T test 2.603E-05 TF0132rp1 number> 0.280 4 TF0132rp1 number <0.230 0 Average (TF0132rp1) / Average (T89) 1,068 Maximum (TF0132rp1) / Maximum (T89) 1,059

[00386] The TF0132Rp1 construction group has a significant density 870170074044, of 29/09/2017, p. 332/378

321/362 sing higher (according to criteria 1 and 2) (+ 7% on average) than the corresponding T89 group. The density change (compared to T89) for the 5 strains in the TF0132Rp1 construction group (3 individuals of each strain), TF0132Rp1-1B (+ 5% on average), TF0132Rp1-3BB (+ 4% on average), TF0132Rp1-4AC (+ 9% on average), TF0132Rp1-4B (+ 9% on average) and TF0132Rp1-6B (+ 7% on average). The TF0132Rp1-4B strain itself meets criteria 1 and 2. Construction group TF0146

TF0146 raw density data

TF0146 Density Name of individual: (g / cm ³ ) TF0146-1A 0.341 TF0146-1B 0.314 TF0146-2A 0.303 TF0146-2B 0.300 TF0146-3A 0.333 TF0146-3B 0.313 TF0146-4A 0.374 TF0146-4B 0.269

Summary of density of TF0146

TF0146 TF0146 Average: 0.318 TF0146 Maximum: 0.374 TF0146 Minimum: 0.269 TF0146 number: 8 Number of TF0146 strains:

T89 Average: 0.274 Maximum T89: 0.354 T89 Min: 0.226 T89 Number: 36

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Confidence interval (95%) 0.274 +/- 0.052

T test 1,127E-04 TF0146 number> 0.326 3 TF0146 number <0.222 0 Average (TF0146) / Average (T89) 1,161 Maximum (TF0146) / Maximum (T89) 1,054

[00387] The TF0146 construction group has a significantly higher density (according to criteria 1 and 2) (+ 16% on average) than the corresponding T89 group.

Construction group TF0173

TF0173 raw density data

TF0173 Density Name of individual: (g / cm ³ ) TF0173-3A-1 0.232 TF0173-3A-2 0.291 TF0173-3B-1 0.239 TF0173-3B-2 0.242 TF0173-4A-1 0.251 TF0173-4A-2 0.221 TF0173-4B-1 0.256 TF0173-4B-2 0.305

Summary of density of TF0173

TF0173 TF0173 Average: 0.256 TF0173 Maximum: 0.305 TF0173 Minimum: 0.221 TF0173 Number: 8 Number of TF0173 strains:

T89 Average: 0.254 Maximum T89: 0.345

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Τ89 Minimum: 0.221

T89 Number: 34 Confidence interval (95%) 0.254 +/- 0.056

T test 8,478E-01 TF0173 number> 0.31 0 TF0173 number <0.198 0 Average (TF0173) / Average (T89) 1.008 Maximum (TF0173) / Maximum (T89) 0.884

[00388] The TF0173 construction group has no significant difference in density (according to criteria 1 and 2) compared to the corresponding T89 group.

Construction group TF0247

TF0247 density raw data

TF0247 Density Name of Individual: (g / cm ³ ) TF0247-1A 0.257 TF0247-3A 0.250 TF0247-3B 0.309 TF0247-4A 0.288 TF0247-6B 0.366

Summary of density of TF0247

TF0247 TF0247 Average: 0.294 TF0247 Maximum: 0.366 TF0247 Minimum: 0.250 TF0247 Number: 5 Number of TF0247 strains:

T89 Average: 0.274 Maximum T89: 0.354 Minimum T89: 0.226

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T89 Number: 36 Confidence interval (95%) 0.274 +/- 0.052

T test 1,498E-01 TF0247 number> 0.326 1 TF0247 number <0.222 0 Average (TF0247) / Average (T89) 1,073 Maximum (TF0247) / Maximum (T89) 1,032

[00389] The TF0247 construction group has no significant difference in density (according to criteria 1 and 2) compared to the corresponding T89 group.

Construction group TF0405

TF0405 raw density data

TF0405 Density Name of individual: (g / cm ³ ) TF0405-2A-1 0.271 TF0405-2A-2 0.291 TF0405-2B-2 TF0405-3A-1 0.275 TF0405-3A-2 0.309 TF0405-3B-1 0.295 TF0405-3B-2 0.279

Summary of density of TF0405

TF0405 TF0405 Average: 0.287 TF0405 Maximum: 0.309 TF0405 Minimum: 0.271 TF0405 Number: 6 Number of TF0405 strains:

T89 Average: 0.274 Maximum T89: 0.354

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Τ89 Minimum: 0.226

T89 Number: 36 Confidence interval (95%) 0.274 +/- 0.052

T test 2,560E-01 TF0405 number> 0.326 0 TF0405 number <0.222 0 Average (TF0405) / Average (T89) 1,045 Maximum (TF0405) / Maximum (T89) 0.871

[00390] The TF0405 construction group has no significant difference in density (according to criteria 1 and 2) compared to the corresponding T89 group.

Construction group TFSTT004

TFSTT004 raw density data

TFSTT004 Density Name of Individual: (g / cm ³ ) TFSTT004-1 ³ 0.300 TFSTT004-2A-1 0.285 TFSTT004-2A-2 0.283 TFSTT004-2B-1 0.272 TFSTT004-2B-2 0.262 TFSTT004-3B 0.329 TFSTT004-4B-1 0.266 TFSTT004-4B-2 0.274 Summary of TFSTT0C density 4

TFSTT004 TFSTT004 Average: 0.284 TFSTT004 Maximum: 0.329 TFSTT004 Minimum: 0.262 TFSTT004 Number: 8 Number of lines TFSTT004:

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T89 Average: 0.275 Maximum T89: 0.345 Minimum T89: 0.223 T89 Number: 23 Confidence interval (95%) 0.275 +/- 0.069

T test 4,777E-01 TFSTT004 number> 0.343 0 TFSTT004 number <0.206 0 Average (TFSTT004) / Average (T89) 1,033 Maximum (TFSTT004) / Maximum (T89) 0.955

[00391] The TFSTT004 construction group has no significant difference in density (according to criteria 1 and 2) compared to the corresponding T89 group.

Construction group TFSTT013

TFSTT013 raw density data

TFSTT013 Density Name of individual: (g / cm ³ ) TFSTT013-1A 0.323 TFSTT013-1B 0.328 TFSTT013-2B 0.288 TFSTT013-3 ³ 0.345 TFSTT013-3B 0.291 TFSTT013-4 ³ 0.304 TFSTT013-4B 0.324 TFSTT013-5B 0.346

Summary of TFSTT013 density

TFSTT013 TFSTT013 Average: 0.319 TFSTT013 Maximum: 0.346

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TFSTT013 Minimum: 0.288

TFSTT013 Number: 8 Number of strains TFSTT013:

T89 Average: 0.284 Maximum T89: 0.361 Minimum T89: 0.222 T89 Number: 41 Confidence interval (95%) 0.284 +/- 0.06

T test 3.006E-03 TFSTT013 number> 0.344 2 TFSTT013 number <0,224 0 Average (TFSTT013) / Average (T89) 1,123 Maximum (TFSTT013) / Maximum (T89) 0.958

[00392] The TFSTT013 construction group has significantly higher density (according to criteria 1 and 2) (+ 12% on average) than the corresponding T89 group.

Construction group TFSTT016

TFSTT016 raw density data

TFSTT016 Density Name of individual: (g / cm ³ ) TFSTT016-1A 0.306 TFSTT016-1B 0.254 TFSTT016-2A 0.249 TFSTT016-2B 0.279 TFSTT016-3A-1 0.272 TFSTT016-3A-2 0.274 TFSTT016-4A 0.252

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Summary of density of TFSTT016

TFSTT016 TFSTT016 Average: 0.270 TFSTT016 Maximum: 0.306 TFSTT016 Minimum: 0.249 TFSTT016 Number: 7 Number of strains TFSTT016:

T89 Average: 0.275 Maximum T89: 0.345 Minimum T89: 0.223 T89 Number: 23 Confidence interval (95%) 0.275 +/- 0.069

T test 6,884E-01 TFSTT016 number> 0.343 0 TFSTT016 number <0.206 0 Average (TFSTT016) / Average (T89) 0.980 Maximum (TFSTT016) / Maximum (T89) 0.886

[00393] The TFSTT016 construction group has no significant difference in density (according to criteria 1 and 2) compared to the corresponding T89 group.

Construction group TFSTT019

TFSTT019 raw density data

TFSTT019 Density Name of individual: (g / cm ³ ) TFSTT019-1 ³ TFSTT019-1BA 0.216 TFSTT019-1BB 0.283 TFSTT019-2 ³ 0.265 TFSTT019-2B 0.221

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TFSTT019-3 ³ 0.277

TFSTT019-4BA 0.244 TFSTT019-4BB 0.282

Summary of TFSTT019 density

TFSTT019 TFSTT019 Average: 0.257 TFSTT019 Maximum: 0.283 TFSTT019 Minimum: 0.216 TFSTT019 Number: 7 Number of TFSTT019 strains:

T89 Average: 0.260 Maximum T89: 0.358 Minimum T89: 0.218 T89 Number: 45 Trust number (95%) 0.26 +/- 0.049

T test 7.862E-01 TFSTT019 Number> 0.309 0 TFSTT019 number <0.211 0 Average (TFSTT019) / Average (T89) 0.989 Maximum (TFSTT019) / Maximum (T89) 0.789

[00394] The TFSTT019 construction group has no significant difference in density (according to criteria 1 and 2) compared to the corresponding T89 group.

Construction group TFSTT035

TFSTT035 raw density data

TFSTT035 Density Name of individual: (g / cm ³ ) TFSTT035-1A 0.236 TFSTT035-1BA 0.260

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TFSTT035-1BB 0.265 TFSTT035-2AA 0.247 TFSTT035-2AB 0.257 TFSTT035-2B 0.217 TFSTT035-3B 0.240 TFSTT035-4B 0.267

Summary of density of TF0104

TFSTT035 TFSTT035 Average: 0.249 TFSTT035 Maximum: 0.267 TFSTT035 Minimum: 0.217 TFSTT035 Number: 8 Number of lines TFSTT035:

T89 Average: 0.260 Maximum T89: 0.358 Minimum T89: 0.218 T89 Number: 45 Confidence interval (95%) 0.26 +/- 0.049

T test 2,247E-01 TFSTT035 number> 0.309 0 TFSTT035 number <0.211 0 Average (TFSTT035) / Average (T89) 0.957 Maximum (TFSTT035) / Maximum (T89) 0.745

[00395] The TFSTT035 construction group has no significant difference in density (according to criteria 1 and 2) compared to the corresponding T89 group.

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Construction group TFSTT036

TFSTT036 raw density data

TFSTT036 Density Name of individual: (g / cm ³ ) TFSTT036-1B 0.291 TFSTT036-2A 0.306 TFSTT036-2B 0.365 TFSTT036-3A 0.322 TFSTT036-4A 0.274 TFSTT036-4B 0.331 TFSTT036-5B 0.254 Summary of TFSTT03 density 6

TFSTT036 TFSTT036 Average: 0.306 TFSTT036 Maximum: 0.365 TFSTT036 Minimum: 0.254 TFSTT036 Number: 7 Number of lines TFSTT036:

T89 Average: 0.274 Maximum T89: 0.354 Minimum T89: 0.226 T89 Number: 36 Confidence interval (95%) 0.274 +/- 0.052

T test 7,640E-03 TFSTT036 number> 0.326 2 TFSTT036 number <0.222 0 Average (TFSTT036) / Average (T89) 1,117 Maximum (TFSTT036) / Maximum (T89) 1,030

[00396] The TFSTT036 construction group has significant density 870170074044, of 29/09/2017, p. 343/378

332/362 sing higher (according to criteria 1 and 2) (+ 12% on average) than the corresponding T89 group.

Construction group TFSTT038

TFSTT038 raw density data

TFSTT038 Density Name of individual: (g / cm ³ ) TFSTT038-1A-1 0.256 TFSTT038-1A-2 0.292 TFSTT038-1B 0.312 TFSTT038-2A 0.308 TFSTT038-2B 0.264 TFSTT038-3A 0.301 TFSTT038-3B 0.343 TFSTT038-4B 0.284 Summary of TFSTT03 density 8

TFSTT038 TFSTT038 Average: 0.295 TFSTT038 Maximum: 0.343 TFSTT038 Minimum: 0.256 TFSTT038 Number: 8 Number of lines TFSTT038:

T89 Average: 0.275 Maximum T89: 0.345 Minimum T89: 0.223 T89 Number: 23 Confidence interval (95%) 0.275 +/- 0.069

T test 1,343E-01 TFSTT038 number> 0.343 0 TFSTT038 number <0.206 0 Average (TFSTT038) / Average (T89) 1,073

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Maximum 0.995 (TFSTT038) / Maximum (T89)

[00397] The TFSTT038 construction group has no significant difference in density (according to criteria 1 and 2) compared to the corresponding T89 group.

Construction group TFSTT045

TFSTT045 raw density data

TFSTT045 Density Name of individual: (g / cm ³ ) TFSTT045-1B 0.221 TFSTT045-2B 0.243 TFSTT045-3A 0.258 TFSTT045-3B 0.257 TFSTT045-4A 0.258 TFSTT045-4B 0.349 TFSTT045-7B 0.324

Summary of TFSTT045 density

TFSTT045 TFSTT045 Average: 0.274 TFSTT045 Maximum: 0.349 TFSTT045 Minimum: 0.221 TFSTT045 Number: 7 Number of lines TFSTT045:

T89 Average: 0.274 Maximum T89: 0.354 Minimum T89: 0.226 T89 Number: 36 Confidence interval (95%) 0.274 +/- 0.052

T test 9,934E-01 TFSTT045 number> 0.326 1

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TFSTT045 number <0.222 0

Average (TFSTT045) / Average (T89) 1,000 Maximum (TFSTT045) / Maximum (T89) 0.984

[00398] The TFSTT045 construction group has no significant difference in density (according to criteria 1 and 2) compared to the corresponding T89 group.

Construction group TFSTT051

TFSTT051 raw density data

TFSTT051 Density Name of individual: (g / cm ³ ) TFSTT051-1B 0.315 TFSTT051-2A 0.318 TFSTT051-2B 0.292 TFSTT051-3A 0.289 TFSTT051-3B 0.270 TFSTT051-4A 0.240 TFSTT051-4B-1 0.269 TFSTT051-4B-2 0.234

Summary of density of TFSTT051

TFSTT051 TFSTT051 Average: 0.278 TFSTT051 Maximum: 0.318 TFSTT051 Minimum: 0.234 TFSTT051 Number: 8 Number of lines TFSTT051:

T89 Average: 0.275 Maximum T89: 0.345 Minimum T89: 0.223 T89 Number: 23 Confidence interval (95%) 0.275 +/- 0.069

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T test 7,969E-01 TFSTT051 number> 0.343 0 TFSTT051 number <0.206 0 Average (TFSTT051) / Average (T89) 1,013 Maximum (TFSTT051) / Maximum (T89) 0.921

[00399] The TFSTT051 construction group has no significant difference in density (according to criteria 1 and 2) compared to the corresponding T89 group.

Example 6

Fiber measurements [00400] Fiber measurements were made on samples at 33 to 36 cm in height of the trunk. A piece of pure wood, about 1.5 mm x 1.5 mm x 15 mm, was cut from the trunk piece. A maceration preparation (Franklin et al. 1945) was carried out to obtain a macerate of simple fibers from the small piece of wood. The sample was then measured using Metso Automation's KajaaniFibreLab®, giving the average fiber length, average fiber width and an estimate of the cell's cell wall thickness. The supplied computer software calculates these numbers using the formulas below according to the manufacturer.

Fiber length [00401] Average fiber length, L (n), using the actual fiber length, measured along the center line:

V (n * L)

Σ ^η >

[mm] [00402] [00403] [00404] where rij = number of class i fibers, / = 1 ... 152,

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336/362 [00405] h = (0.05 * i) -0.025, [00406] Ij = length of class i.

[00407] Fiber width [00408] Average fiber width, W; based on cross-sectional measurements:

[ _M m] [00409] where [00410] / ?, = number of fibers in class /, [00411] /=1...100, [00412] = kw * (i-0,5), [00413 ] Wj = class width /, [00414] kw = width calibration factor

Cell wall thickness [pm] [00415] where [00416] / ?, = number of fibers in class i, [00417] /=1...100, [00418] CI / l / 7} = kt * ( i-0.5), [00419] CWTí = class i cell wall thickness, [00420] kt = wall thickness calibration factor.

[00421] Construction Groups with fibers with at least a 10% increase or a 15% increase in length or width of the fibers were selected as being carried out on genes useful for modifying the fiber size according to the selection criteria below .

[00422] Criteria for selecting fiber parameters [00423] In Table 6.1, abbreviations used for the phenotypes used in the fiber selection criteria are listed.

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AFL average fiber length of the wild type population and each population of the Construction Group

AFW average fiber width of the wild type population and each population of the MaxFL Construction Group maximum length of the wild type population and each population of the MaxFW Construction Group maxFW maximum fiber width of the wild type population and each population of the MinFL Construction Group minimum fiber length of the wild type population and each population of the MinFW Construction Group Minimum fiber width of the wild type population and each population of the construction group [00424] The construction groups that showed difference compared to the type population wild in any of the fiber parameters mentioned above were classified as construction groups that are altered in their properties and, therefore, the corresponding genes can be used to alter these properties.

[00425] As a 10% increase or a 15% increase in fiber dimensions is of interest to the industry, the selection criteria below have been used to select genes that can be used for altered fiber dimensions.

[00426] The criteria for selecting fiber parameters are as shown below:

[00427] If the AFL of the construction group is at least 10% greater than the AFL of the corresponding wild type group, or [00428] If the AFW of the construction group is at least 10% greater than the AFW of the type group corresponding wild, or [00429] If the maxFL of the construction group is at least 10% greater than the maxFL of the corresponding wild type group, or

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338/362 [00430] If the maxFW of the construction group is at least 10% greater than the maxFw of the wild type group, or [00431] If the AFL length of the construction group is at least 15% less than the AFL of the group of the corresponding wild type, or [00432] If the AFW of the construction group is at least 15% less than the minFL of the corresponding wild group, or [00433] If the minFW of the construction group is at least 15% less than the minFL of the group corresponding wild.

[00434] If the minFW of the construction group is at least 15% lower than the minFW of the corresponding wild group.

[00435] Construction groups meeting one or more of these criteria were selected.

[00436] The results below are presented according to the construction group.

Construction group TF0002 [00437] This construction induces changes in the fiber parameters. The maximum fiber width is 16% greater than the corresponding maximum wild type. Construction group TF0002 fulfills criterion (4) for selecting the corresponding fiber parameters.

[00438] Table 6.2 contains fiber measurement data for the specified construction group and the corresponding wild type group.

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M 3.87 3.5 3.3 3.63 3.63 3.71 3.39 3.93 3.46 3.36 3.8 Fiber Width (pm) 16.03 14.89 14.11 15.44 15.34 15.56 14.43 16.4 14.68 14.25 15.94 Length of Fiber (mm) 0.37 0.36 0.37 co co o 0.37 0.37 0.36 0.37 0.37 0.33 00 co o Construction Group Individual T89-27 T89-3 T89-30 T89-31 T89-32 T89-34 T89-36 T89-4 T89-6 T89-7 T89-9

M 4.32 4.8 't co 3.9 3.51 3.85 3.37 3.23 3.37 3.34 Width of Fiber (pm) 17.73 CD 14.57 16.23 14.71 15.86 14.2 14.1 14.57 14.39 Fiber Length (mm) 0.37 0.39 0.34 0.36 0.34 0.36 0.33 0.35 0.35 0.36 Construction Group Individual TF0002-1B TF0002-2A TF0002-2B TF0002-3A TF0002-3B TF0002-4B T89-1 T89-18 T89-20 T89-24

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340/362 [00439] The results of the fiber measurements are shown in Table 6.3 as ratios of the average fiber length (AFL), average fiber width (AFW), maximum fiber length (maxFL), maximum fiber width (maxFW ), minimum fiber length (minFL), minimum fiber width (minFW) of the specified building group with respect to the corresponding wild group.

Table 6.3

Group of Construction Average Fiber Length Average Fiber Width Average Fiber Width Maximum Fiber Width Minimum Fiber Length Minimum Fiber Width TF0002 1.00 1.09 1.03 1.16 1.03 1.03

Construction group TF0052 [00440] This construction induces changes in fiber parameters. The maximum fiber width is 13% greater than the corresponding wild type group. The maximum fiber width is 22% greater than the corresponding maximum wild type. Construction group TF0002 fulfills criteria (2) and (4) for selecting fiber parameters.

[00441] Table 6.4 contains fiber measurement data for the specified construction group and the corresponding wild type group.

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3.28 3.17 3.58 3.14 3.39 3.58 3.31 3.12 3.13 3.18 2.91 Fiber Width (pm) 14.25 13.82 15.11 13.42 14.54 15.2 14.12 13.52 13.41 13.3 12.52 Fiber Length (mm) 0.34 0.33 0.36 0.34 0.33 0.37 0.35 0.33 0.33 0.3 0.32 Construction Group Individual T89-18 T89-19 T89-20 T89-21 T89-24 T89-25 T89-26 T89-27 T89-28 T89-3 T89-30

'and Ξ2. H% O 3.11 3.46 3.86 4.49 4.71 3.49 3.9 3.76 3.59 't CO 2.83 3.14 Fiber Width (pm) 12.77 14.4 15.9 18.22 18.54 14.52 15.94 15.33 15.15 14.79 12.52 13.67 Fiber Length (mm) 0.3 0.33 0.34 0.36 0.34 0.34 0.35 0.32 0.35 0.34 0.33 0.34 Construction Group Individual TF0052-1A TF0052-1B TF0052-2A TF0052-2B TF0052-3A TF0052-3B TF0052-4A TF0052-4B T89-1 T89-12 T89-14 T89-16

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342/362 [00442] The results of the fiber measurements are shown in Table 6.3 as ratios of the average fiber length (AFL), average fiber width (AFW), maximum fiber length (maxFL), maximum fiber width (maxFW ), minimum fiber length (minFL), minimum fiber width (minFW) of the specified construction group with respect to the corresponding wild group.

Table 6.5

Group of Construction Average Fiber Length Average Fiber Width Average Fiber Width Maximum Fiber Width Minimum Fiber Length Minimum Fiber Width TF0052 0.99 1.13 0.97 1.22 1.00 1.02

Construction group TF0058 [00443] This construction induces changes in the fiber parameters. The maximum fiber width is 16% greater than the corresponding wild type group. The maximum fiber width is 23% greater than the corresponding maximum wild type. Construction group TF0058 fulfills criteria (2) and (4) for selecting fiber parameters.

[00444] Table 6.6 contains fiber measurement data for the specified construction group and the corresponding wild type group.

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M 3.28 3.17 3.58 3.14 3.39 3.58 3.31 3.12 3.13 3.18 2.91 Fiber Width (pm) 14.25 13.82 15.11 13.42 14.54 15.2 14.12 13.52 13.41 13.3 12.52 Fiber Length (mm) 0.34 0.33 0.36 0.34 0.33 0.37 0.35 0.33 0.33 0.3 0.32 Construction Group Individual T89-18 T89-19 T89-20 T89-21 T89-24 T89-25 T89-26 T89-27 T89-28 T89-3 T89-30

'and Ξ2. H% O 3.74 3.66 4.21 3.89 3.67 3.77 4.14 4.57 3.59 't CO 2.83 3.14 Width of Fiber (pm) 15.29 15.07 16.98 15.89 15.28 15.62 16.97 18.7 15.15 14.79 12.52 13.67 Length of Fiber (mm) 0.34 0.31 0.36 0.34 0.36 0.36 0.37 0.39 0.35 0.34 0.33 0.34 Construction Group Individual < 1 WITH LL H TF0058-1B TF0058-2A TF0058-2B TF0058-3A TF0058-3B < 1 with the LL H TF0058-4B T89-1 T89-12 T89-14 T89-16

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344/362 [00445] The results of the fiber measurements are shown in Table 6.7 as ratios of the average fiber length (AFL), average fiber width (AFW), maximum fiber length (maxFL), maximum fiber width (maxFW ), minimum fiber length (minFL), minimum fiber width (minFW) of the specified construction group with respect to the corresponding wild group.

Table 6.7

Group of Construction Average Fiber Length Average Fiber Width Average Fiber Width Maximum Fiber Width Minimum Fiber Length Minimum Fiber Width TF0058 1.05 1.16 1.05 1.23 1.03 1.20

Construction Group TF0097 [00446] This construction induces changes in the fiber parameters. The maximum fiber width is 13% greater than the corresponding maximum wild type. Construction group TF0002 fulfills criterion (4) for selecting the corresponding fiber parameters.

[00447] Table 6.8 contains fiber measurement data for the specified construction group and the corresponding wild type group.

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M 3.67 2.7 3.63 CO 3.17 2.96 3.12 3.52 3.24 3.26 3.35 2.99 Width of Fiber (pm) 15.14 12.06 15.19 14.51 13.73 12.93 13.28 14.69 13.64 13.96 14.09 12.49 Length of Fiber (mm) 0.32 0.32 0.36 0.36 0.35 0.33 0.32 0.34 0.32 0.34 0.34 0.29 Group Individual T89-19 T89-26 T89-29 T89-31 T89-32 T89-34 T89-35 T89-38 T89-44 T89-46 T89-61 T89-68

'and Ξ2. H% O 4.07 3.53 2.92 3.8 4.13 3.37 2.79 2.98 2.9 2.7 3.25 3.2 3.15 Width of Fiber (pm) 16.6 14.84 12.65 15.92 17.11 14.05 12.27 13.03 12.34 11.82 14.1 13.66 13.26 Length of Fiber (mm) 00 co O 0.36 0.33 0.37 00 co O 0.33 0.34 0.33 0.3 0.3 0.36 0.35 0.31 Construction Group Individual TF0097-1A TF0097-1B TF0097-2A TF0097-2B TF0097-3A-1 TF0097-3A-2 TF0097-4A-1 TF0097-4A-2 TF0097-4B T89-02 T89-07 T89-11 T89-17

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346/362 [00448] The results of fiber measurements are shown in Table 6.9 as ratios of average fiber length (AFL), average fiber width (AFW), maximum fiber length (maxFL), maximum fiber width (maxFW ), minimum fiber length (minFL), minimum fiber width (minFW) of the specified construction group with respect to the corresponding wild group.

Table 6.9

Group of Construction Average Fiber Length Average Fiber Width Average Fiber Width Maximum Fiber Width Minimum Fiber Length Minimum Fiber Width TF0097 1.04 1.05 1.06 1.13 1.03 1.04

Construction Group TF0109 [00449] This construction induces changes in the fiber parameters. The average fiber length is 11% greater than the corresponding wild type group. The maximum fiber length is 25% greater than the corresponding maximum wild type. The maximum fiber width is 23% greater than the corresponding maximum wild type. Construction group TF0109 fulfills criteria (1), (3) and (4) for selecting fiber parameters.

[00450] Table 6.10 contains fiber measurement data for the specified construction group and the corresponding wild type group.

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M CN 't CO 3.46 3.73 3.61 3.54 3.77 4.23 4.02 3.23 4.44 3.55 Width of Fiber (pm) 14.43 14.52 15.48 14.93 14.68 15.84 16.92 15.95 13.64 17.48 14.5 Length of Fiber (mm) 0.33 0.34 0.35 0.32 0.33 0.36 0.31 0.31 0.3 0.33 0.3 Construction Group Individual T89-20 T89-24 T89-25 T89-26 T89-34 T89-35 T89-36 T89-37 T89-38 T89-4 T89-6

M 3.54 3.8 3.69 3.75 3.75 5.12 poo 3.82 3.79 3.9 3.25 Fiber Width (pm) 15.06 15.83 15.4 15.7 15.73 21.49 16.82 15.93 15.56 15.87 13.88 Length of Fiber (mm) 0.35 0.35 0.34 0.35 0.36 m o 0.37 0.35 0.34 0.34 0.33 Construction Group Individual TF0109-1B-1 TF0109-1B-2 TF0109-2A TF0109-2B TF0109-3B TF0109-4A TF0109-4B T89-16 T89-17 T89-19 T89-2

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348/362 [00451] The results of the fiber measurements are shown in Table 6.11 as ratios of the average fiber length (AFL), average fiber width (AFW), maximum fiber length (maxFL), maximum fiber width (maxFW ), minimum fiber length (minFL), minimum fiber width (minFW) of the specified construction group with respect to the corresponding wild group.

Table 6.11

Group of Construction Average Fiber Length Average Fiber Width Average Fiber Width Maximum Fiber Width Minimum Fiber Length Minimum Fiber Width TF0109 1.11 1.08 1.25 1.23 1.13 1.10

Construction Group TF0116 [00452] This construction induces changes in the fiber parameters. The average fiber width is 11% greater than the corresponding wild type group. The maximum fiber width is 20% greater than the corresponding maximum wild type. Construction group TF0116 meets criteria (2) and (4) for selecting fiber parameters.

[00453] Table 6.12 contains fiber measurement data for the specified construction group and the corresponding wild type group.

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3.28 3.17 3.58 3.14 3.39 3.58 3.31 3.12 3.13 3.18 2.91 Fiber Width (pm) 14.25 13.82 15.11 13.42 14.54 15.2 14.12 13.52 13.41 13.3 12.52 Length of Fiber (mm) 0.34 0.33 0.36 0.34 0.33 0.37 0.35 0.33 0.33 0.3 0.32 Construction Group Individual T89-18 T89-19 T89-20 T89-21 T89-24 T89-25 T89-26 T89-27 T89-28 T89-3 T89-30

M 3.55 4.52 CO 3.76 2.82 3.69 3.94 3.59 't CO 2.83 3.14 Fiber Width (pm) 14.84 18.24 15.66 15.85 12.15 15.2 16.5 15.15 14.79 12.52 13.67 Length of Fiber (mm) 0.33 0.34 0.34 0.37 0.33 0.33 0.37 0.35 0.34 0.33 0.34 Construction Group Individual TF0116-2A TF0116-2B-1 TF0116-2B-2 < 1 CD The LL H TF0116-5B TF0116-6A TF0116-6B T89-1 T89-12 T89-14 T89-16

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350/362 [00454] The results of the fiber measurements are shown in Table 6.13 as ratios of the average fiber length (AFL), average fiber width (AFW), maximum fiber length (maxFL), maximum fiber width (maxFW ), minimum fiber length (minFL), minimum fiber width (minFW) of the specified construction group with respect to the corresponding wild group.

Table 6.13

Group of Construction Average Fiber Length Average Fiber Width Average Fiber Width Maximum Fiber Width Minimum Fiber Length Minimum Fiber Width TF0116 1.02 1.11 1.00 1.20 1.10 0.97

Construction Group TFST001 [00455] This construction induces changes in the fiber parameters. The average fiber width is 14% smaller than the corresponding wild type group. The minimum fiber width is 17% smaller than the corresponding minimum wild type. The minimum fiber width is 30% less than the corresponding minimum wild type. Construction group TFSTT001 fulfills criteria (7) and (8) for selecting fiber parameters.

[00456] Table 6.14 contains fiber measurement data for the specified construction group and the corresponding wild type group.

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'and Ξ2. H% O CN 't CO 3.46 3.73 3.61 3.54 3.77 4.23 4.02 3.23 4.44 3.55 Width of Fiber (pm) 14.43 14.52 15.48 14.93 14.68 15.84 16.92 15.95 13.64 17.48 14.5 Fiber Length (mm) 0.33 0.34 0.35 0.32 0.33 0.36 0.31 0.31 0.3 0.33 0.3 Construction Group Individual T89-20 T89-24 T89-25 T89-26 T89-34 T89-35 T89-36 T89-37 T89-38 T89-4 T89-6

'and Ξ2. H% O 2.7 2.83 2.51 3.06 3.65 3.63 3.77 3.82 3.79 3.9 3.25 Width of Fiber (pm) 11.68 12.11 9.57 12.87 15.23 15.26 15.66 15.93 15.56 15.87 13.88 Length of Fiber (mm) 0.31 0.3 0.25 0.28 0.36 0.35 0.35 0.35 0.34 0.34 0.33 Construction Group Individual TFSTT001-1BX-2 TFSTT001-1BY-1 TFSTT001-1BY-2 TFSTT001-2A-1 TFSTT001-2A-2 TFSTT001-3B-1 TFSTT001-3B-2 T89-16 T89-17 T89-19 T89-2

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352/362 [00457] The results of the fiber measurements are shown in Table 6.15 as ratios of the average fiber length (AFL), average fiber width (AFW), maximum fiber length (maxFL), maximum fiber width (maxFW ), minimum fiber length (minFL), minimum fiber width (minFW) of the specified construction group with respect to the corresponding wild group.

Table 6.15

Group of Construction Average Fiber Length Average Fiber Width Average Fiber Width Maximum Fiber Width Minimum Fiber Length Minimum Fiber Width TFSTT0 010002 0.95 0.86 1.00 0.90 0.83 0.70

Construction Group TFSTT004 [00458] This construction induces changes in the fiber parameters. The average fiber width is 15% greater than the corresponding wild type group. The maximum fiber width is 29% greater than the corresponding maximum wild type. Construction group TFSTT004 fulfills criteria (2) and (4) for selecting fiber parameters.

[00459] Table 6.16 contains fiber measurement data for the specified construction group and the corresponding wild type group.

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'and Ξ2. H% O 3.28 3.17 3.58 3.14 3.39 3.58 3.31 3.12 3.13 3.18 2.91 Width of Fiber (pm) 14.25 13.82 15.11 13.42 14.54 15.2 14.12 13.52 13.41 13.3 12.52 Length of Fiber (mm) 0.34 0.33 0.36 0.34 0.33 0.37 0.35 0.33 0.33 0.3 0.32 Construction Group Individual T89-18 T89-19 T89-20 T89-21 T89-24 T89-25 T89-26 T89-27 T89-28 T89-3 T89-30

'and Ξ2. H% O 3.63 3.84 4.92 3.86 3.53 4.13 4.1 3.88 3.59 't CO 2.83 3.14 Width of Fiber (One) 14.81 16.07 19.54 CD 14.67 14.73 16.72 16.21 15.15 14.79 12.52 13.67 Length of Fiber (mm) 0.31 0.35 0.37 0.37 0.35 0.3 0.35 0.37 0.35 0.34 0.33 0.34 Individual of Group of construction TFSTT004-1A TFSTT004-2A-1 TFSTT004-2A-2 TFSTT004-2B-1 TFSTT004-2B-2 TFSTT004-3B TFSTT004-4B-1 TFSTT004-4B-2 T89-1 T89-12 T89-14 T89-16

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354/362 [00460] The results of the fiber measurements are shown in Table 6.3 as ratios of the average fiber length (AFL), average fiber width (AFW), maximum fiber length (maxFL), maximum fiber width (maxFW ), minimum fiber length (minFL), minimum fiber width (minFW) of the specified construction group with respect to the corresponding wild group.

Table 6.17

Group of Construction Average Fiber Length Average Fiber Width Average Fiber Width Maximum Fiber Width Minimum Fiber Length Minimum Fiber Width TFSTT0 04 1.03 1.15 1.00 1.29 1.00 1.17

Construction Group TFSTT017 [00461] This construction induces changes in the fiber parameters. The minimum fiber width is 17% smaller than the corresponding minimum wild type. Construction group TFSTT017 fulfills criterion (7) for selecting the corresponding fiber parameters.

[00462] Table 6.18 contains fiber measurement data for the specified construction group and the corresponding wild type group.

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M 3.28 3.17 3.58 3.14 3.39 3.58 3.31 3.12 3.13 3.18 2.91 Fiber Width (pm) 14.25 13.82 15.11 13.42 14.54 15.2 14.12 13.52 13.41 13.3 12.52 Fiber Length (mm) 0.34 0.33 0.36 0.34 0.33 0.37 0.35 0.33 0.33 0.3 0.32 Construction Group Individual T89-18 T89-19 T89-20 T89-21 T89-24 T89-25 T89-26 T89-27 T89-28 T89-3 T89-30

'and Ξ2. H% O 3.63 3.84 4.92 3.86 3.53 4.13 4.1 3.88 3.59 't CO 2.83 3.14 Width of Fiber (pm) 14.81 16.07 19.54 CD 14.67 14.73 16.72 16.21 15.15 14.79 12.52 13.67 Length of Fiber (mm) 0.31 0.35 0.37 0.37 0.35 0.3 0.35 0.37 0.35 0.34 0.33 0.34 Individual of Group of construction TFSTT004-1A TFSTT004-2A-1 TFSTT004-2A-2 TFSTT004-2B-1 TFSTT004-2B-2 TFSTT004-3B TFSTT004-4B-1 TFSTT004-4B-2 T89-1 T89-12 T89-14 T89-16

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356/362 [00463] The results of the fiber measurements are shown in Table 6.3 as ratios of the average fiber length (AFL), average fiber width (AFW), maximum fiber length (maxFL), maximum fiber width (maxFW ), minimum fiber length (minFL), minimum fiber width (minFW) of the specified construction group with respect to the corresponding wild group.

Table 6.19

Group of Construction Average Fiber Length Average Fiber Width Average Fiber Width Maximum Fiber Width Minimum Fiber Length Minimum Fiber Width TFSTT0 7 0.93 0.98 1.03 1.05 0.83 0.95

Construction Group TFSTT038 [00464] This construction induces changes in the fiber parameters. The average fiber width is 16% greater than the corresponding wild type group. The maximum fiber width is 21% greater than the corresponding maximum wild type. Construction group TFSTT038 fulfills criteria (2) and (4) for selecting the corresponding fiber parameters.

[00465] Table 6.20 contains fiber measurement data for the specified construction group and the corresponding wild type group.

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'and 0. H% O 3.28 3.17 3.58 3.14 3.39 3.58 3.31 3.12 3.13 3.18 2.91 Fiber Width (pm) 14.25 13.82 15.11 13.42 14.54 15.2 14.12 13.52 13.41 13.3 12.52 Fiber Length (mm) 0.34 0.33 0.36 0.34 0.33 0.37 0.35 0.33 0.33 0.3 0.32 Construction Group Individual T89-18 T89-19 T89-20 T89-21 T89-24 T89-25 T89-26 T89-27 T89-28 T89-3 T89-30

'and 0. H% O 4.1 4.27 4.55 4.04 3.93 3.56 2.98 3.59 't C * 3 2.83 5 Ê and the CO 00 00 1 ^ ID ID CD ID CD CXI 00 CO 't CD CD 00 T— 1 ^ ID D)> -Q CD CD 1 ^ 00 CD ID 't CXI ID 't CXI O GJ A c The g -Q 'and ID ID CD 1 ^ 't CXI CD CXI ID 't CO LL CO CO CO CO CO CO CO CO CO CO CO Ui rω Yeah GJ AND, O O O O O O O O O O O Q. 0  ° (V THE CXI A 0Q < 0Q < 0Q 0Q 0 0 < < T— CXI CXI CO CO 't o * = 1 1 CO 00 00 00 00 CO CXI 0 w CO 00 CO CO CO CO CO CO CO CO O O O O O O CD 1 O) 00 | _ 1 O) 00 | _ 2nd O O H H H H H H 00 D ° 1- 1- H H H H H H H > Φ 0 H H ω ω ω ω ω ω (/) (/) LL LL LL LL LL LL 1 to LL H LL H H H H H H H

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358/362 [00466] The results of the fiber measurements are shown in Table 6.21 as ratios of the average fiber length (AFL), average fiber width (AFW), maximum fiber length (maxFL), maximum fiber width (maxFW ), minimum fiber length (minFL), minimum fiber width (minFW) of the specified construction group with respect to the corresponding wild group.

Table 6.21

Group of Construction Average Fiber Length Average Fiber Width Average Fiber Width Maximum Fiber Width Minimum Fiber Length Minimum Fiber Width TFSTT0 38 1.03 1.16 1.00 1.21 1.07 1.03

Example 7

Selected buildings used for processing tobacco

Tobacco transformation [00467] Based on the growth data from experiments with black beech, a selected set of constructions, that is, CaMV 35S: overexpression of DNA constructions TF0097, TF0132 and TFSTT019, was used for transformation into tobacco ( Nicotiana tabacum cv. SR1). The plants were transformed and regenerated essentially as described by Nilsson et al. (1992), but using leaf disc explants.

[00468] Approximately 10-15 independent strains were generated for each construction. One of each group of transgenic plants produced using a construction will hereinafter be called a construction group, for example, different transgenic plants that emanate from a construction. Each transgenic strain within each construction group, for example, TF05555-02, TF0555-03 and so on are different transformation events and therefore have the recombinant DNA inserted in the different locations

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359/362 in the plant genome. This makes different strains within a building group partly different. For example, it is known that different transformation events will produce plants with different levels of genetic overexpression.

Plant Growth [00469] Transgenic tobacco plants, comprising three construction groups, each of which has 9 to 15 independent strains, were grown together with 14 wild-type control plants, in a greenhouse under an 18-hour photoperiod and a temperature of 22 ° C / 18 ° C (day / night). The plants were fertilized with NPK 71-5, Weibulls Rika S, diluted from 1 to 10 (final concentrations were: NO ₃ , 55 g / L; NH ₄ , 29 g / L; P, 12 g / L; K, 56 g / L; Mg 7.2 g / L; S, 7.2 g / L; B, 0.18 g / L; Cu, 0.02 g / L; Fe, 0.84 g / L; Mn, 0.42 g / L; Mo, 0.03 g / L; Zn, 0.13 g / L). The height and diameter of the plant were measured regularly during growth in the greenhouse.

[00470] The growth effects observed in tobacco transformants included faster regeneration of tobacco plants transformed with the TF0132 construct, where regenerated seedlings had markedly wider leaves during the early tissue culture phases. Also, in plants transformed with any of the selected constructions (ie TF0097, TF0132, or TFSTT019), a long period of vegetative growth and, later, in flowering, was observed than wild type SR1 plants.

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Claims (4)

1. Method for producing a transgenic perennial woody plant showing increased growth measured by the punch weight of the trunk compared to its wild type, characterized by the fact that it comprises overexpressing in the transgenic perennial woody plant a gene product of a gene comprising a sequence of heterologous nucleotides according to SEQ ID NO: 10, said method comprising the step of providing a recombinant DNA construct comprising a heterologous nucleotide sequence according to SEQ ID NO: 10, the steps of said method comprising: (i) providing an expression vector comprising a heterologous nucleotide sequence according to SEQ ID NO: 10, and a regulatory element operably linked to the polynucleotide sequence, wherein said regulatory element controls the expression of the heterologous polynucleotide sequence on a target perennial woody plant; (ii) introducing the expression vector into a perennial woody plant; and (iii) selecting a transgenic perennial woody plant that has increased growth as measured by the dry weight of the trunk compared to its wild type; or (i) selecting a transgenic perennial woody plant species that expresses a heterologous nucleotide sequence according to SEQ ID NO: 10, (ii) crossing a transgenic perennial woody plant species selected in (i) with the same or another transgenic perennial woody plant species selected in (i), (iii) select transgenic perennial woody plants with increased expression of a heterologous nucleotide sequence from Petition 870180166238, of 12/21/2018, p. 8/15 2/2 according to SEQ ID NO: 10, compared to the transgenic perennial woody plant species selected in (i); (iv) backcross, optionally, one or more times the transgenic perennial woody plants obtained in (iii) and select transgenic perennial woody plants with increased expression of a heterologous nucleotide sequence according to SEQ ID NO: 10, in comparison with any one of the species of transgenic perennial woody plants used in (i) and / or transgenic perennial woody plants obtained in (iii). Method according to claim 1, characterized in that the construction of recombinant DNA further comprises a constitutive, inducible, or tissue-specific promoter operably linked to said heterologous nucleotide sequence. Method according to claim 2, characterized in that the recombinant DNA construct comprises the constitutive promoter CaMV 35S operably linked to said heterologous nucleotide sequence. 4. Method according to claim 2 or 3, characterized in that the method comprises the additional step of transforming regenerable cells from a perennial woody plant with said recombinant DNA construction and regenerating a transgenic perennial woody plant from said transformed cell.