BR112022002647A2 - Método para ajustar a concentração de glicose a um valor alvo durante o cultivo de células de mamífero - Google Patents

Método para ajustar a concentração de glicose a um valor alvo durante o cultivo de células de mamífero

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Publication number
BR112022002647A2
BR112022002647A2 BR112022002647A BR112022002647A BR112022002647A2 BR 112022002647 A2 BR112022002647 A2 BR 112022002647A2 BR 112022002647 A BR112022002647 A BR 112022002647A BR 112022002647 A BR112022002647 A BR 112022002647A BR 112022002647 A2 BR112022002647 A2 BR 112022002647A2
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Prior art keywords
cell culture
mammalian cell
target value
value during
glucose concentration
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BR112022002647A
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English (en)
Inventor
Daniel Stefke
Kristina Erhard
Sriram Venkateswaran
Tobias Grosskopf
Wolfgang Paul
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Hoffmann La Roche
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Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of BR112022002647A2 publication Critical patent/BR112022002647A2/pt

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    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
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    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • G—PHYSICS
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    • G06N—COMPUTING ARRANGEMENTS BASED ON SPECIFIC COMPUTATIONAL MODELS
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    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

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  • Chemical & Material Sciences (AREA)
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Abstract

método para ajustar a concentração de glicose a um valor alvo durante o cultivo de células de mamífero. sistemas de cultivo de alto rendimento são usados em pesquisa e desenvolvimento farmacêutico. nesta conexão, amostras são coletadas e analisadas para parâmetros importantes usando análise externa. os resultados da análise servem para avaliar o processo de cultivo e fornecer informações importantes sobre o processo. especialmente com cultivos realizados em paralelo, o esforço manual de preparo das amostras é grande e pode levar a erros. a fim de evitar a necessidade de amostragem e, assim, minimizar os erros, um método é descrito no presente pedido de patente que torna os parâmetros alvo desejados acessíveis na forma de sensores suaves por meio de variáveis de processo previamente registradas. aqui é descrito um método para determinar parâmetros relevantes para o processo em processos cho (ovário de hamster chinês) em cultivos de alto rendimento, em particular glicose, lactato e a densidade de células vivas ou o volume de células vivas.
BR112022002647A 2019-08-14 2020-08-12 Método para ajustar a concentração de glicose a um valor alvo durante o cultivo de células de mamífero BR112022002647A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP19191807 2019-08-14
PCT/EP2020/072560 WO2021028453A1 (en) 2019-08-14 2020-08-12 Method for determining process variables in cell cultivation processes

Publications (1)

Publication Number Publication Date
BR112022002647A2 true BR112022002647A2 (pt) 2022-05-03

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Country Status (11)

Country Link
US (1) US20220306979A1 (pt)
EP (1) EP4013848A1 (pt)
JP (2) JP7410273B2 (pt)
KR (1) KR20220032599A (pt)
CN (1) CN114223034A (pt)
AU (1) AU2020330701B2 (pt)
BR (1) BR112022002647A2 (pt)
CA (1) CA3145252A1 (pt)
IL (1) IL290500A (pt)
MX (1) MX2022001822A (pt)
WO (1) WO2021028453A1 (pt)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113406251B (zh) * 2021-06-29 2022-05-17 江南大学 预测白酒储存年份的方法
KR20230060189A (ko) * 2021-10-27 2023-05-04 프레스티지바이오로직스 주식회사 인공지능을 이용하여 세포 배양조건을 결정하기 위한 장치 및 장치의 동작 방법
WO2024055008A1 (en) * 2022-09-09 2024-03-14 Genentech, Inc. Prediction of viability of cell culture during a biomolecule manufacturing process
CN116926241A (zh) * 2023-06-30 2023-10-24 广东美赛尔细胞生物科技有限公司 一种免疫细胞培养控制方法及系统

Also Published As

Publication number Publication date
WO2021028453A1 (en) 2021-02-18
AU2020330701B2 (en) 2023-06-29
MX2022001822A (es) 2022-03-17
JP7410273B2 (ja) 2024-01-09
AU2020330701A1 (en) 2022-02-10
EP4013848A1 (en) 2022-06-22
US20220306979A1 (en) 2022-09-29
CA3145252A1 (en) 2021-02-18
JP2024038006A (ja) 2024-03-19
CN114223034A (zh) 2022-03-22
KR20220032599A (ko) 2022-03-15
IL290500A (en) 2022-04-01
JP2022544928A (ja) 2022-10-24

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