BR112021024923A2 - Methods of preparing a cell line, producing a pharmaceutical product, obtaining a cell line and producing a protein, producing and hybrid cell lines, system for producing high level pharmaceutical products, and, pharmaceutical product - Google Patents
Methods of preparing a cell line, producing a pharmaceutical product, obtaining a cell line and producing a protein, producing and hybrid cell lines, system for producing high level pharmaceutical products, and, pharmaceutical productInfo
- Publication number
- BR112021024923A2 BR112021024923A2 BR112021024923A BR112021024923A BR112021024923A2 BR 112021024923 A2 BR112021024923 A2 BR 112021024923A2 BR 112021024923 A BR112021024923 A BR 112021024923A BR 112021024923 A BR112021024923 A BR 112021024923A BR 112021024923 A2 BR112021024923 A2 BR 112021024923A2
- Authority
- BR
- Brazil
- Prior art keywords
- producing
- protein
- cell lines
- cell line
- preparing
- Prior art date
Links
- 210000004027 cell Anatomy 0.000 title abstract 14
- 108090000623 proteins and genes Proteins 0.000 title abstract 7
- 102000004169 proteins and genes Human genes 0.000 title abstract 6
- 239000000825 pharmaceutical preparation Substances 0.000 title abstract 4
- 229940127557 pharmaceutical product Drugs 0.000 title abstract 4
- 210000004754 hybrid cell Anatomy 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- -1 and Substances 0.000 title 1
- 238000004519 manufacturing process Methods 0.000 abstract 3
- 239000000203 mixture Substances 0.000 abstract 2
- 229960000074 biopharmaceutical Drugs 0.000 abstract 1
- 239000012531 culture fluid Substances 0.000 abstract 1
- 239000003814 drug Substances 0.000 abstract 1
- 210000002472 endoplasmic reticulum Anatomy 0.000 abstract 1
- 230000004927 fusion Effects 0.000 abstract 1
- 210000002288 golgi apparatus Anatomy 0.000 abstract 1
- 239000008177 pharmaceutical agent Substances 0.000 abstract 1
- 230000001225 therapeutic effect Effects 0.000 abstract 1
- 230000014616 translation Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
- C12N5/12—Fused cells, e.g. hybridomas
- C12N5/16—Animal cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/02—Cells for production
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Gastroenterology & Hepatology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
métodos de preparação de uma linhagem de células produtoras, de obtenção e preparação de uma linha celular e de produção de um produto farmacêutico e de uma proteína para composição como um biofármaco, linhas de células produtoras e híbridas, população de células produtoras, sistema para produção de produtos, e, produto farmacêutico.esta descrição provê linhas celulares melhoradas para a fabricação de agentes farmacêuticos à base de proteínas, reduzindo consideravelmente o custo de produção comercial. as linhas celulares são obtidas selecionando células de uma população mista para uma ou mais características que suportam a produção de proteínas em uma base não específica, como o nível de retículo endoplasmático, aparelho de golgi e/ou outras características fenotípicas desejadas, em comparação com outras células na mistura inicial. as linhas celulares produtoras particularmente eficazes podem ser obtidas preparando as células para seleção funcional, fazendo híbridos de células. um gene que codifica uma proteína terapêutica de interesse pode ser transfectado nas células antes ou depois de um ou mais ciclos de fusão e seleção. dependendo do produto proteico a ser expresso, podem ser obtidas linhas celulares que produzem oito gramas ou mais de proteína por litro de fluido de cultura.methods of preparing a producer cell line, obtaining and preparing a cell line and producing a pharmaceutical product and a protein for composition as a biopharmaceutical, producer and hybrid cell lines, population of producer cells, system for production of products, and pharmaceuticals. This description provides improved cell lines for the manufacture of protein-based pharmaceutical agents, considerably reducing the cost of commercial production. cell lines are obtained by selecting cells from a mixed population for one or more traits that support protein production on a non-specific basis, such as the level of endoplasmic reticulum, golgi apparatus, and/or other desired phenotypic traits, compared to others cells in the initial mixture. particularly effective producer cell lines can be obtained by preparing the cells for functional selection by making cell hybrids. a gene encoding a therapeutic protein of interest can be transfected into cells before or after one or more rounds of fusion and selection. depending on the protein product to be expressed, cell lines that produce eight grams or more of protein per liter of culture fluid can be obtained.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/US2019/036379 WO2020251537A1 (en) | 2019-06-10 | 2019-06-10 | Cell lines for high level production of protein-based pharmaceuticals |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112021024923A2 true BR112021024923A2 (en) | 2022-02-15 |
Family
ID=73780999
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112021024923A BR112021024923A2 (en) | 2019-06-10 | 2019-06-10 | Methods of preparing a cell line, producing a pharmaceutical product, obtaining a cell line and producing a protein, producing and hybrid cell lines, system for producing high level pharmaceutical products, and, pharmaceutical product |
Country Status (9)
| Country | Link |
|---|---|
| EP (1) | EP3980524A4 (en) |
| JP (2) | JP2022543522A (en) |
| KR (1) | KR102648479B1 (en) |
| CN (1) | CN114096659A (en) |
| AU (1) | AU2019450349B2 (en) |
| BR (1) | BR112021024923A2 (en) |
| CA (1) | CA3143154A1 (en) |
| IL (1) | IL288877B2 (en) |
| WO (1) | WO2020251537A1 (en) |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4912040A (en) * | 1986-11-14 | 1990-03-27 | Genetics Institute, Inc. | Eucaryotic expression system |
| US6136599A (en) * | 1998-12-10 | 2000-10-24 | Bayer Corporation | Human hybrid host cell for mammalian gene expression |
| EP1196566B1 (en) * | 1999-07-12 | 2006-02-01 | Genentech, Inc. | Expression vectors and methods |
| US7033781B1 (en) * | 1999-09-29 | 2006-04-25 | Diversa Corporation | Whole cell engineering by mutagenizing a substantial portion of a starting genome, combining mutations, and optionally repeating |
| WO2006027202A1 (en) * | 2004-09-06 | 2006-03-16 | Unite De Recherche En Biotherapie Et Oncologie (Rubio) | Generation of multiparent cell hybrids |
| US7351582B2 (en) * | 2005-06-17 | 2008-04-01 | Avigenics, Inc. | Cell lines |
| WO2009103753A1 (en) * | 2008-02-20 | 2009-08-27 | Ablynx Nv | Methods for identifying and/or sorting cells by secreted molecule and kits for performing such methods |
| US20120015841A1 (en) * | 2009-02-02 | 2012-01-19 | Chromocell Corporation | Novel cell lines and methods |
| DK2440653T3 (en) * | 2009-06-10 | 2019-03-18 | Bts Res International Pty Ltd | Methods for generating hybrid / chimeric cells and their applications |
| US10329594B1 (en) * | 2015-09-03 | 2019-06-25 | CHO Plus, Inc. | Cell lines for high level production of protein-based pharmaceuticals |
| CA3067972A1 (en) * | 2017-06-16 | 2018-12-20 | Lonza Ltd | Universal self-regulating mammalian cell line platform for the production of biologics |
-
2019
- 2019-06-10 EP EP19933147.1A patent/EP3980524A4/en active Pending
- 2019-06-10 JP JP2021573362A patent/JP2022543522A/en active Pending
- 2019-06-10 IL IL288877A patent/IL288877B2/en unknown
- 2019-06-10 WO PCT/US2019/036379 patent/WO2020251537A1/en active Search and Examination
- 2019-06-10 BR BR112021024923A patent/BR112021024923A2/en unknown
- 2019-06-10 KR KR1020227000886A patent/KR102648479B1/en active IP Right Grant
- 2019-06-10 CA CA3143154A patent/CA3143154A1/en active Pending
- 2019-06-10 AU AU2019450349A patent/AU2019450349B2/en active Active
- 2019-06-10 CN CN201980097383.XA patent/CN114096659A/en active Pending
-
2024
- 2024-04-05 JP JP2024061743A patent/JP2024091682A/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP3980524A1 (en) | 2022-04-13 |
| AU2019450349B2 (en) | 2022-05-26 |
| CN114096659A (en) | 2022-02-25 |
| KR102648479B1 (en) | 2024-03-15 |
| IL288877B1 (en) | 2024-01-01 |
| WO2020251537A1 (en) | 2020-12-17 |
| IL288877A (en) | 2022-07-01 |
| CA3143154A1 (en) | 2020-12-17 |
| IL288877B2 (en) | 2024-05-01 |
| AU2019450349A1 (en) | 2022-01-27 |
| EP3980524A4 (en) | 2023-01-11 |
| JP2022543522A (en) | 2022-10-13 |
| JP2024091682A (en) | 2024-07-05 |
| KR20220024478A (en) | 2022-03-03 |
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