BR112021023101A2 - Method for expanding adult stem cells from whole blood - Google Patents

Method for expanding adult stem cells from whole blood

Info

Publication number
BR112021023101A2
BR112021023101A2 BR112021023101A BR112021023101A BR112021023101A2 BR 112021023101 A2 BR112021023101 A2 BR 112021023101A2 BR 112021023101 A BR112021023101 A BR 112021023101A BR 112021023101 A BR112021023101 A BR 112021023101A BR 112021023101 A2 BR112021023101 A2 BR 112021023101A2
Authority
BR
Brazil
Prior art keywords
stem cells
adult stem
whole blood
blood
expanding
Prior art date
Application number
BR112021023101A
Other languages
Portuguese (pt)
Inventor
Marco Polettini
Original Assignee
Thankstem S R L
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Thankstem S R L filed Critical Thankstem S R L
Publication of BR112021023101A2 publication Critical patent/BR112021023101A2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/14—Blood; Artificial blood
    • A61K35/16—Blood plasma; Blood serum
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0644—Platelets; Megakaryocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/02—Atmosphere, e.g. low oxygen conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/22—Colony stimulating factors (G-CSF, GM-CSF)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

método para expansão de células-tronco adultas do sangue total. método para expandir células-tronco adultas a partir do sangue, compreendendo o crescimento e a desprogramação das células-tronco adultas de uma amostra de sangue, por meio de tratamento in vitro da amostra de sangue com um produto derivado de sangue à base de prp que inclui mcsf autólogo obtido por lise de plaquetas em uma porção da mesma amostra de sangue autóloga.method for expanding adult stem cells from whole blood. method for expanding adult stem cells from blood, comprising growing and deprogramming adult stem cells from a blood sample, by in vitro treatment of the blood sample with a prp-based blood product that includes autologous mcsf obtained by lysis of platelets in a portion of the same autologous blood sample.

BR112021023101A 2019-05-22 2020-05-19 Method for expanding adult stem cells from whole blood BR112021023101A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IT201900007076 2019-05-22
PCT/IT2020/050126 WO2020234910A1 (en) 2019-05-22 2020-05-19 Method for expanding adult stem cells from whole blood

Publications (1)

Publication Number Publication Date
BR112021023101A2 true BR112021023101A2 (en) 2022-01-04

Family

ID=67875993

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112021023101A BR112021023101A2 (en) 2019-05-22 2020-05-19 Method for expanding adult stem cells from whole blood

Country Status (6)

Country Link
EP (1) EP3973051A1 (en)
CN (1) CN113924361A (en)
AU (1) AU2020277774A1 (en)
BR (1) BR112021023101A2 (en)
MX (1) MX2021014118A (en)
WO (1) WO2020234910A1 (en)

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005065419A2 (en) * 2003-12-29 2005-07-21 Am Biosolutions Method of culturing cells
ITRM20060498A1 (en) * 2006-09-20 2008-03-21 Alessandra Gambacurta METHOD OF EXPANSION OF ADULT STEM CELLS FROM PERIPHERAL BLOOD AND RELATED USES IN MEDICAL FIELD
IT1392897B1 (en) * 2008-07-18 2012-04-02 Fond Ircss Ca' Granda - Ospedale Maggiore Policlinico PIASTRINIC FRACTION DERIVING FROM PLACENTED BLOOD
CA2840568C (en) * 2011-06-27 2021-05-04 Emory University Compositions, uses, and preparation of platelet lysates
ITRM20110500A1 (en) * 2011-09-23 2013-03-24 Futura Stem Cells Sa LYSATE PIASTRINICO, USE OF IT AND METHOD FOR ITS PREPARATION
CN102367435B (en) * 2011-11-04 2013-01-16 四川新生命干细胞科技股份有限公司 Preparation of human platelet-rich plasma and application of same in isolation and culture of human mesenchymal stem cells
CN102533641B (en) * 2012-02-20 2016-08-17 光丽生医股份有限公司 External serum-free adult stem cell amplifies the method and nutrient solution thereof cultivated
US20140356893A1 (en) * 2013-06-04 2014-12-04 Allan Mishra Compositions and methods for using platelet-rich plasma for drug discovery, cell nuclear reprogramming, proliferation or differentiation
HUE040661T2 (en) * 2014-05-09 2019-03-28 Thankstem S R L Method for expanding adult stem cells from whole blood
CA3033750A1 (en) * 2016-08-29 2018-03-08 Hackensack University Medical Center Compositions and methods for programming adult cells with platelet rich fraction of blood containing platelet-like cells

Also Published As

Publication number Publication date
WO2020234910A1 (en) 2020-11-26
CN113924361A (en) 2022-01-11
MX2021014118A (en) 2021-12-10
EP3973051A1 (en) 2022-03-30
AU2020277774A1 (en) 2021-12-09

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