BR112017008873A2 - expression cassette for eukaryotic cell transformation, process for eukaryotic cell transformation, genetically modified microorganism, biofuel and / or biochemical production process and biofuel and / or biochemical production thus produced - Google Patents

expression cassette for eukaryotic cell transformation, process for eukaryotic cell transformation, genetically modified microorganism, biofuel and / or biochemical production process and biofuel and / or biochemical production thus produced

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Publication number
BR112017008873A2
BR112017008873A2 BR112017008873A BR112017008873A BR112017008873A2 BR 112017008873 A2 BR112017008873 A2 BR 112017008873A2 BR 112017008873 A BR112017008873 A BR 112017008873A BR 112017008873 A BR112017008873 A BR 112017008873A BR 112017008873 A2 BR112017008873 A2 BR 112017008873A2
Authority
BR
Brazil
Prior art keywords
biofuel
eukaryotic cell
cell transformation
biochemical production
expression cassette
Prior art date
Application number
BR112017008873A
Other languages
Portuguese (pt)
Inventor
Luzia Drezza Angela
Amarante Guimarães Pereira Gonçalo
Vieira Dos Santos Leandro
Carolina De Barros Grassi Maria
Original Assignee
Biocelere Agroindustrial Ltda
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biocelere Agroindustrial Ltda filed Critical Biocelere Agroindustrial Ltda
Publication of BR112017008873A2 publication Critical patent/BR112017008873A2/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/22—Processes using, or culture media containing, cellulose or hydrolysates thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

a presente invenção descreve um cassete de expressão para transformar célula eucariótica que compreende a sequência de nucleotídeos (seq id no: 2) codificadora de peptídeo com característica xilose isomerase (seq id no:1), opcionalmente compreendendo também outros genes da via das pentoses fosfato. a invenção descreve também micro-organismo geneticamente modificado pela inserção do cassete de expressão descrito, capaz de consumir xilose e converter etanol quando comparado ao seu correspondente sem as ditas modificações. descreve-se também processo para produção de biocombustíveis e bioquímicos, preferencialmente etanol, a partir de açúcares abundantes na porção lignocelulósica da biomassa vegetal, principalmente pentoses e, entre as pentoses, principalmente xilose. são descritos também biocombustíveis, preferencialmente etanol, e bioquímicos produzidos pelo processo da invenção.The present invention describes an expression cassette for transforming eukaryotic cells comprising the nucleotide sequence (sequence id: 2) encoding peptide with characteristic xylose isomerase (sequence id: 1), optionally also comprising other phosphate pathway genes. . The invention also describes genetically modified microorganism by inserting the described expression cassette capable of consuming xylose and converting ethanol as compared to its counterpart without said modifications. A process for producing biofuels and biochemicals, preferably ethanol, is also described from sugars abundant in the lignocellulosic portion of the plant biomass, mainly pentoses and, among the pentoses, mainly xylose. Also described are biofuels, preferably ethanol, and biochemicals produced by the process of the invention.

BR112017008873A 2014-10-30 2015-10-30 expression cassette for eukaryotic cell transformation, process for eukaryotic cell transformation, genetically modified microorganism, biofuel and / or biochemical production process and biofuel and / or biochemical production thus produced BR112017008873A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
BR102014027233A BR102014027233A2 (en) 2014-10-30 2014-10-30 expression cassette, genetically modified microorganism for xylose isomerase expression, process for production of biofuels and / or biochemicals and biofuel and / or biochemicals produced
PCT/BR2015/050198 WO2016065453A1 (en) 2014-10-30 2015-10-30 Expression cassette for eukaryotic cell transformation, method for transforming eukaryotic cells, genetically modified microorganism, method for producing biofuels and/or biochemicals, and biofuel and/or biochemical thereby produced

Publications (1)

Publication Number Publication Date
BR112017008873A2 true BR112017008873A2 (en) 2018-01-16

Family

ID=55856300

Family Applications (2)

Application Number Title Priority Date Filing Date
BR102014027233A BR102014027233A2 (en) 2014-10-30 2014-10-30 expression cassette, genetically modified microorganism for xylose isomerase expression, process for production of biofuels and / or biochemicals and biofuel and / or biochemicals produced
BR112017008873A BR112017008873A2 (en) 2014-10-30 2015-10-30 expression cassette for eukaryotic cell transformation, process for eukaryotic cell transformation, genetically modified microorganism, biofuel and / or biochemical production process and biofuel and / or biochemical production thus produced

Family Applications Before (1)

Application Number Title Priority Date Filing Date
BR102014027233A BR102014027233A2 (en) 2014-10-30 2014-10-30 expression cassette, genetically modified microorganism for xylose isomerase expression, process for production of biofuels and / or biochemicals and biofuel and / or biochemicals produced

Country Status (2)

Country Link
BR (2) BR102014027233A2 (en)
WO (1) WO2016065453A1 (en)

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60325457D1 (en) * 2002-01-23 2009-02-05 Royal Nedalco B V FERMENTATION OF PENTOSE SUGAR
PL1626979T3 (en) * 2003-05-02 2012-09-28 Cargill Inc Genetically modified yeast species and fermentation processes using genetically modified yeast
SE0302421D0 (en) * 2003-09-11 2003-09-11 Forskarpatent I Syd Ab Construction of new xylose utilizing Saccharomyces cerevisiae strain
CA2573483C (en) * 2004-07-16 2014-10-28 Technische Universiteit Delft Metabolic engineering of xylose-fermenting eukaryotic cells
US9334488B2 (en) * 2008-12-24 2016-05-10 Dsm Ip Assets B.V. Xylose isomerase genes and their use in fermentation of pentose sugars
CN102174549B (en) * 2011-02-22 2012-10-10 山东大学 Nucleic acid molecules for coding xylose isomerase and xylose isomerase coded by same
US9090889B2 (en) * 2012-07-24 2015-07-28 Bp Corporation North America Inc. Xylose isomerases and their uses
US9441249B2 (en) * 2012-08-24 2016-09-13 National University Corporation Kobe University Method for producing ethanol from biomass
BR112015011466A8 (en) * 2012-11-20 2018-05-02 Codexis Inc RECOMBINANT FUNGAL HOST CELL, RECOMBINANT NUCLEIC ACID CONSTRUCT, ISOLATED POLYPEPTIDE SEQUENCE, AND, METHODS FOR PRODUCING A FERMENTATION PRODUCT AND AT LEAST ONE FINAL PRODUCT FROM AT LEAST ONE CELLULOSIC SUBSTRATE
US8669076B1 (en) * 2013-03-11 2014-03-11 E I Du Pont De Nemours And Company Cow rumen xylose isomerases active in yeast cells

Also Published As

Publication number Publication date
WO2016065453A1 (en) 2016-05-06
BR102014027233A2 (en) 2016-05-24
WO2016065453A9 (en) 2016-12-15

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