BR112017002752A2 - dispositivo para selecionar células eucarióticas (sp), uso do dispositivo para selecionar células eucarióticas (sp), procedimento para selecionar in vitro células eucarióticas (sp) e sêmen sexado - Google Patents

dispositivo para selecionar células eucarióticas (sp), uso do dispositivo para selecionar células eucarióticas (sp), procedimento para selecionar in vitro células eucarióticas (sp) e sêmen sexado

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Publication number
BR112017002752A2
BR112017002752A2 BR112017002752-6A BR112017002752A BR112017002752A2 BR 112017002752 A2 BR112017002752 A2 BR 112017002752A2 BR 112017002752 A BR112017002752 A BR 112017002752A BR 112017002752 A2 BR112017002752 A2 BR 112017002752A2
Authority
BR
Brazil
Prior art keywords
eukaryotic cell
cell selection
selection device
transport channel
electromagnetic radiation
Prior art date
Application number
BR112017002752-6A
Other languages
English (en)
Inventor
Liegeois Luc
Courtade Emmanuel
Damart Hervé
Pesez Jean
Thommen Quentin
Treizebre Anthony
Original Assignee
Genes Diffusion
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genes Diffusion filed Critical Genes Diffusion
Publication of BR112017002752A2 publication Critical patent/BR112017002752A2/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/0612—Germ cells sorting of gametes, e.g. according to sex or motility
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434—Optical arrangements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1484—Optical investigation techniques, e.g. flow cytometry microstructural devices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/42—Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Engineering & Computer Science (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

o dispositivo pode ser usado para a seleção in vitro de células eucarióticas, particularmente espermatozoides (sp). ele compreende um canal de transporte (100), de preferência um canal de transporte de tr (100), no qual uma solução contendo tais células eucarióticas pode circular (sp), uma primeira passagem transversal (104) desembocando em tal canal de transporte (100), uma fonte de radiação eletromagnética (60), acoplada à primeira extremidade de uma primeira fibra óptica (61), com a outra extremidade de emissão (61b) da primeira fibra óptica inserida em tal primeira passagem transversal (104), sem projetar no canal de transporte (100). o dispositivo compreende ainda meios de controle eletrônicos (7) que possibilitam o controle automático de tal fonte de radiação eletromagnética (60) de modo a alterar seletivamente as células eucarióticas (sp) circulando no canal de transporte (100) através da radiação eletromagnética de alteração (r) emitida pela fonte de radiação eletromagnética (60).
BR112017002752-6A 2014-08-11 2015-08-04 dispositivo para selecionar células eucarióticas (sp), uso do dispositivo para selecionar células eucarióticas (sp), procedimento para selecionar in vitro células eucarióticas (sp) e sêmen sexado BR112017002752A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR1457734A FR3024738B1 (fr) 2014-08-11 2014-08-11 Dispostif et procede de selection de cellules eucaryotes dans un canal de transport par alteration des cellules eucaryotes au moyen d'un rayonnemement electromagnetique
FR1457734 2014-08-11
PCT/FR2015/052154 WO2016024061A1 (fr) 2014-08-11 2015-08-04 Dispostif et procede de selection de cellules eucaryotes dans un canal de transport par alteration des cellules eucaryotes au moyen d'un rayonnemement electromagnetique

Publications (1)

Publication Number Publication Date
BR112017002752A2 true BR112017002752A2 (pt) 2018-01-30

Family

ID=51830526

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112017002752-6A BR112017002752A2 (pt) 2014-08-11 2015-08-04 dispositivo para selecionar células eucarióticas (sp), uso do dispositivo para selecionar células eucarióticas (sp), procedimento para selecionar in vitro células eucarióticas (sp) e sêmen sexado

Country Status (9)

Country Link
US (1) US20170355955A1 (pt)
EP (1) EP3180417B1 (pt)
CN (1) CN106796171A (pt)
BR (1) BR112017002752A2 (pt)
CA (1) CA2957350A1 (pt)
FR (1) FR3024738B1 (pt)
PL (1) PL3180417T3 (pt)
RU (1) RU2017106668A (pt)
WO (1) WO2016024061A1 (pt)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3845885A1 (en) * 2012-07-27 2021-07-07 Engender Technologies Limited Method and system for microfluidic particle orientation and/or sorting
FR3061910A1 (fr) * 2017-01-18 2018-07-20 Genes Diffusion Dispostif et procede de discrimination de spermatozoides
US11738344B2 (en) * 2017-09-19 2023-08-29 Hifibio Sas Particle sorting in a microfluidic system
EP4063835B1 (en) * 2021-03-22 2024-05-29 Rise Research Institutes Of Sweden Ab Fiber arrangement for illumination and detection

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX345105B (es) * 2003-03-28 2017-01-16 Inguran Llc * Método de fotodaño para separar partículas.
NZ530972A (en) * 2004-02-05 2005-04-29 Embrionics Ltd A method and apparatus for orientating and selecting cells
WO2007022641A1 (en) * 2005-08-25 2007-03-01 Institut National D'optique Flow cytometry analysis across optical fiber
WO2009151624A1 (en) * 2008-06-13 2009-12-17 Xy, Inc. Lubricious microfluidic flow path system
PL2304020T3 (pl) * 2008-06-30 2019-06-28 Microbix Biosystems Inc. Sposób i urządzenie do sortowania komórek
HU227875B1 (en) * 2008-10-13 2012-05-29 Diatron Medicinai Instr Laboratoriumi Diagnosztikai Fejlesztoe Gyarto Zartkoerueen Muekoedoe Reszven Optical circulation cytometer furthermore testing method
US20100220315A1 (en) * 2009-02-27 2010-09-02 Beckman Coulter, Inc. Stabilized Optical System for Flow Cytometry
US8891084B2 (en) * 2009-07-07 2014-11-18 Sony Corporation Microfluidic device
EP2671065B1 (en) * 2011-02-04 2019-07-10 Cytonome/ST, LLC Particle sorting apparatus and method
CN103013811A (zh) * 2011-09-20 2013-04-03 北京富通华投资有限公司 精子分选仪

Also Published As

Publication number Publication date
EP3180417B1 (fr) 2019-10-02
FR3024738A1 (fr) 2016-02-12
RU2017106668A (ru) 2018-09-13
EP3180417A1 (fr) 2017-06-21
CA2957350A1 (fr) 2016-02-18
US20170355955A1 (en) 2017-12-14
WO2016024061A1 (fr) 2016-02-18
CN106796171A (zh) 2017-05-31
FR3024738B1 (fr) 2018-06-15
PL3180417T3 (pl) 2020-03-31

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Legal Events

Date Code Title Description
B11A Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing
B11Y Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette]