BR112015031269A2 - Sistema de células integrais para a biocatálise de mono-oxigenases de citocromo p450 - Google Patents

Sistema de células integrais para a biocatálise de mono-oxigenases de citocromo p450

Info

Publication number
BR112015031269A2
BR112015031269A2 BR112015031269A BR112015031269A BR112015031269A2 BR 112015031269 A2 BR112015031269 A2 BR 112015031269A2 BR 112015031269 A BR112015031269 A BR 112015031269A BR 112015031269 A BR112015031269 A BR 112015031269A BR 112015031269 A2 BR112015031269 A2 BR 112015031269A2
Authority
BR
Brazil
Prior art keywords
cytochrome
whole cell
oxygenases
biocatalysis
mono
Prior art date
Application number
BR112015031269A
Other languages
English (en)
Inventor
Gerber Adrian
Hannemann Frank
Bleif Sabrina
Kleser Michael
Bernhardt Rita
Original Assignee
Sanofi Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sanofi Sa filed Critical Sanofi Sa
Publication of BR112015031269A2 publication Critical patent/BR112015031269A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/12—Acting on D ring
    • C12P33/16—Acting at 17 position
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
    • C12N9/0081—Cholesterol monooxygenase (cytochrome P 450scc)(1.14.15.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0095—Oxidoreductases (1.) acting on iron-sulfur proteins as donor (1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C12P7/625—Polyesters of hydroxy carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/15—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen (1.14.15)
    • C12Y114/15006—Cholesterol monooxygenase (side-chain-cleaving) (1.14.15.6), i.e. cytochrome P450scc
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)

Abstract

SISTEMA DE CÉLULAS INTEGRAIS PARA A BIOCATÁLISE DE MONO-OXIGENASES DE CITOCROMO P450. A presente invenção refere-se a um processo de catálise de células integrais para converter substratos de mono-oxigenases de citocromo P450 de origem eucariótica em produtos biotecnológicos valiosos. O objeto da presente invenção também se refere a micro-organismos projetados geneticamente para obter essas biotransformações com razões elevadas e processos para a preparação dessas cepas de micro-organismos.
BR112015031269A 2013-06-17 2014-06-17 Sistema de células integrais para a biocatálise de mono-oxigenases de citocromo p450 BR112015031269A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP13305814 2013-06-17
PCT/EP2014/062749 WO2014202627A2 (en) 2013-06-17 2014-06-17 Whole-cell system for cytochrome p450 monooxygenases biocatalysis

Publications (1)

Publication Number Publication Date
BR112015031269A2 true BR112015031269A2 (pt) 2017-09-19

Family

ID=48699702

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112015031269A BR112015031269A2 (pt) 2013-06-17 2014-06-17 Sistema de células integrais para a biocatálise de mono-oxigenases de citocromo p450

Country Status (13)

Country Link
US (1) US10308972B2 (pt)
EP (1) EP3011045B1 (pt)
JP (1) JP6635917B2 (pt)
KR (1) KR20160019451A (pt)
CN (2) CN105473730A (pt)
AU (1) AU2014283301A1 (pt)
BR (1) BR112015031269A2 (pt)
CA (1) CA2914894A1 (pt)
MX (1) MX2015017531A (pt)
RU (1) RU2684714C2 (pt)
SG (1) SG11201509965YA (pt)
TW (1) TWI654300B (pt)
WO (1) WO2014202627A2 (pt)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11932878B2 (en) 2016-07-07 2024-03-19 University Of Florida Research Foundation, Inc. Metabolically competent cells, methods of making, and uses thereof
WO2018009869A1 (en) * 2016-07-07 2018-01-11 University Of Florida Research Foundation, Inc. Metabolically competent cells, methods of making, and uses thereof
CN107034150B (zh) * 2017-04-13 2021-06-29 天津大学 一种重组耶氏解脂酵母菌株及其构建方法与应用
CN107312787B (zh) * 2017-07-18 2020-04-14 浙江大学宁波理工学院 融合蛋白基因、工程菌及其在制备9α-羟基-雄烯二酮中的应用

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU1090716A1 (ru) 1982-06-02 1984-05-07 Институт биоорганической химии АН БССР Способ получени @ 4- @ или @ прегненолона
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US20030108982A1 (en) * 1988-05-06 2003-06-12 Aventis Pharma S.A. Process for oxidation of steroids and genetically engineered cells used therein
IL90207A (en) * 1988-05-06 1994-07-31 Roussel Uclaf Biochemical oxidation of steroids and genetically engineered cells to be used therefor
EP0477961B1 (en) 1990-09-26 1996-09-11 Sumitomo Chemical Company, Limited Mitochondrial P450
IL162476A (en) 1995-02-15 2005-12-18 Aventis Pharma Sa Arabidopsis thaliana proteins having delta -5,7- sterol - delta -7- reductase activity
US6804725B1 (en) * 1996-08-30 2004-10-12 Texas Instruments Incorporated IC with state machine controlled linking module
GB9704737D0 (en) * 1997-03-07 1997-04-23 Optel Instr Limited Biological measurement system
ATE354600T1 (de) 1997-05-12 2007-03-15 Metabolix Inc Polyhydroxyalkanoate für in vivo anwendungen
MXPA01001750A (es) 1998-08-18 2002-04-08 Metabolix Inc Productos microbianos transgenicos de polihidroxialcanoato.
FR2820145B1 (fr) * 2001-01-31 2004-01-23 Aventis Pharma Sa Souche de levure produisant des steroides de facon autonome
CA2655764A1 (en) 2006-07-11 2008-01-17 Basf Se Protein targeting to lipid bodies
WO2010079594A1 (ja) * 2009-01-07 2010-07-15 三菱化学株式会社 ステロール側鎖切断酵素蛋白質およびその利用

Also Published As

Publication number Publication date
RU2015153538A3 (pt) 2018-03-21
TWI654300B (zh) 2019-03-21
MX2015017531A (es) 2016-04-15
EP3011045A2 (en) 2016-04-27
CA2914894A1 (en) 2014-12-24
RU2684714C2 (ru) 2019-04-11
SG11201509965YA (en) 2016-01-28
EP3011045B1 (en) 2019-10-16
AU2014283301A1 (en) 2016-01-07
CN118109544A (zh) 2024-05-31
WO2014202627A2 (en) 2014-12-24
US20160376625A1 (en) 2016-12-29
RU2015153538A (ru) 2017-07-20
JP2016521578A (ja) 2016-07-25
KR20160019451A (ko) 2016-02-19
TW201522626A (zh) 2015-06-16
CN105473730A (zh) 2016-04-06
US10308972B2 (en) 2019-06-04
JP6635917B2 (ja) 2020-01-29
WO2014202627A3 (en) 2015-03-12

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Legal Events

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B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements